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Summary: In the current work, the effect of pumpkin parts on the oil content, total phenol, flavonoid, carotenoid quantities, antioxidant capacity, phenolic components, fatty acid profiles and mineral profiles of pumpkin parts was investigated. Total carotenoid and phenolic amounts of pumpkin parts were recorded to be from 36.84 (seed) and 29.92 μg g−1 (pulp) to 28.75 (pulp) and 67.68 mgGAE/100 g (peel) respectively. Also, total flavonoid quantities of the parts of pumpkin were assigned between 21.07 mg/100 g (pulp) and 220.36 mg/100 g (seed), while antioxidant activities of pumpkin samples range from 0.04 mmol kg−1 (pulp) to 0.82 mmol kg−1 (seed). Catechin and caffeic acid amounts of pumpkin parts varied from 13.25 (seed) and 77.31 mg/100 g (peel) to 4.70 (seed) and 7.60 mg/100 g (peel) respectively. The amount of catechin detected in the pumpkin peel was approximately two and five times higher than the pulp part. Resveratrol content was lowest in the pulp part of the pumpkin. Oleic and linoleic acid values of the oils ranged from 19.23% (peel) to 29.16% (seed) and from 18.15% (peel) to 51.06% (seed) respectively. Stearic, oleic and linoleic acids of pumpkin pulp oil were higher than pumpkin peel oil. Pumpkin seed oils are rich in oleic and linoleic acids. P, K, Mg, S and Fe were the dominant biogenic minerals of pumpkin parts. The most of total carotenoids and total phenols were accumulated in the peel part of the pumpkin fruit. The total flavonoid quantity of pumpkin seeds was approximately three times higher than the pumpkin peel part and ten times higher than the pulp part. [ABSTRACT FROM AUTHOR]

This study demonstrates a facile solvothermal approach to synthesizing green fluorescent carbon dots (G‐CDs) using baby pumpkin as a natural precursor. The G‐CDs exhibit strong fluorescence emission at 547 nm under excitation at 467 nm, and the highest quantum yield reaching 17.5 % in acetone, making them suitable for a range of applications including sensing, bioimaging, catalysis, and drug delivery. The G‐CDs demonstrate excellent stability under various conditions, such as prolonged light exposure, high salt concentration, extended periods, and elevated temperatures. Furthermore, the G‐CDs show a good linear relationship with Cd2+ within the concentration range of 0 to 16.5 μmol/L, with a linear correlation coefficient of 0.9920. The detection limit (LOD) was calculated to be 0.68 μmol/L. The recovery rate ranges between 85.66% to 97.43%, indicating their potential for use in environmental metal ion detection. This study provides a convenient and economical method for the synthesis of G‐CDs using baby pumpkin as a precursor, offering a sustainable alternative for various applications in sensing, imaging, catalysis, and drug delivery. [ABSTRACT FROM AUTHOR]

Simple Summary: Pumpkins (Cucurbita moschata), valued for their nutritional, medicinal, and economic contributions, are threatened by root-knot nematodes, notably Meloidogyne incognita. This research explores the impact of M. incognita on the growth and comprehensive physiological responses of pumpkins. The findings reveal that infection leads to significant growth impairment, as indicated by reduced plant height and biomass along with the development of nematode-induced galls. In addition, there is an observable oxidative stress response characterized by elevated levels of hydrogen peroxide and an increase in antioxidant defense mechanisms such as crucial antioxidative enzymes (superoxide dismutase, glutathione reductase, and catalase) and the accumulation of glutathione. These responses demonstrate a dynamic interplay between the plant and the nematode, where pumpkins mobilize robust antioxidant defenses to counteract the stress induced by nematode infection. Despite these defense mechanisms, pumpkin's ability to combat M. incognita raises concerns about the agricultural production challenges posed by this pest in Cucurbita crops. The insights gained from this study improve our understanding of plant–nematode interactions, paving the way for strategies aimed at increasing resistance against these pests, thus promoting sustainable agricultural practices. Pumpkins (Cucurbita moschata), valued for their nutritional, medicinal, and economic significance, face threats from Meloidogyne incognita, a critical plant-parasitic nematode. This study extensively examines the impact of M. incognita on the growth, physiological, and biochemical responses of C. moschata. We demonstrate that M. incognita infection leads to significant growth impairment in C. moschata, evidenced by reduced plant height and biomass, along with the significant development of nematode-induced galls. Concurrently, a pronounced oxidative stress response was observed, characterized by elevated levels of hydrogen peroxide and a significant increase in antioxidant defense mechanisms, including the upregulation of key antioxidative enzymes (superoxide dismutase, glutathione reductase, catalase, and peroxidase) and the accumulation of glutathione. These responses highlight a dynamic interaction between the plant and the nematode, wherein C. moschata activates a robust antioxidant defense to mitigate the oxidative stress induced by nematode infection. Despite these defenses, the persistence of growth impairment underscores the challenge posed by M. incognita to the agricultural production of C. moschata. Our findings contribute to the understanding of plant–nematode interactions, paving the way for the development of strategies aimed at enhancing resistance in Cucurbitaceae crops against nematode pests, thus supporting sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Pumpkin (Cucurbita maxima) is an important vegetable crop of the Cucurbitaceae plant family. The fruits of pumpkin are often used as directly edible food or raw material for a number of processed foods. In nature, mature pumpkin fruits differ in size, shape, and color. The Atlantic Giant (AG) cultivar has the world's largest fruits and is described as the giant pumpkin. AG is well-known for its large and bright-colored fruits with high ornamental and economic value. At present, there are insufficient studies that have focused on the formation factors of the AG cultivar. To address these knowledge gaps, we performed comparative transcriptome, proteome, and metabolome analysis of fruits from the AG cultivar and a pumpkin with relatively small fruit (Hubbard). The results indicate that up-regulation of gene-encoded expansins contributed to fruit cell expansion, and the increased presence of photoassimilates (stachyose and D-glucose) and jasmonic acid (JA) accumulation worked together in terms of the formation of large fruit in the AG cultivar. Notably, perhaps due to the rapid transport of photoassimilates, abundant stachyose that was not converted into glucose in time was detected in giant pumpkin fruits, implying that a unique mode of assimilate unloading is in existence in the AG cultivar. The potential molecular regulatory network of photoassimilate metabolism closely related to pumpkin fruit expansion was also investigated, finding that three MYB transcription factors, namely CmaCh02G015900, CmaCh01G018100, and CmaCh06G011110, may be involved in metabolic regulation. In addition, neoxanthin (a type of carotenoid) exhibited decreased accumulation that was attributed to the down-regulation of carotenoid biosynthesis genes in AG fruits, which may lead to pigmentation differences between the two pumpkin cultivars. Our current work will provide new insights into the potential formation factors of giant pumpkins for further systematic elucidation. [ABSTRACT FROM AUTHOR]

Gluten‐free bakery products are the definitive solution for people with celiac disease and gluten sensitivity. In this study, the production of gluten‐free sponge cake was optimized using a mixture design methodology. Effects of the amount of ingredients, including quinoa (Chenopodium quinoa) (6–10 g), oleaster (Eleagnus Angustifolia) (1–2 g), and pumpkin powder (Cucurbita moschata) (1–4 g) on the physicochemical characteristics and sensory qualities (color, flavor, cutability, texture, appearance, and overall acceptability) of cakes were investigated. Significant regression models that explained the effects of different amounts of flour on all response variables were determined. The proposed model in this study had high R2 and R2 (adj). Therefore, the model was approved for fitting information. Finally, a gluten‐free sponge cake recipe was formulated using 1 g of oleaster flour, 1 g of pumpkin, and 10 g of quinoa flour to achieve the desired sensory quality. [ABSTRACT FROM AUTHOR]

Flower colour, as an important morphological marker, plays an essential role in improving the identification efficiency of the purity seed in hybrid production. However, the molecular mechanism of white‐flower trait has not been reported in pumpkin (Cucurbita moschata D.). In this study, we obtained a white‐flower mutant (wf) through the ethyl methane sulfonate (EMS) mutagenesis of inbred line N87 (yellow flower). F2 populations were then constructed by crossing wf mutant and N87 plant to fine map the genes controlling white‐flower trait in pumpkin. Phenotypic identification revealed that carotenoid content significantly decreased in the petals of wf mutants compared with N87 plants. Genetic analysis indicated that the white flower mutant trait was controlled by a single recessive gene, Cmowf. Using bulked segregant analysis and KASP phenotyping, Cmowf was mapped to a 762 kb region on chromosome 14 containing three annotated genes. Among them, a nonsynonymous single‐nucleotide polymorphisms mutation was identified only in CmoCh14G005820 gene, which encoded a DUF1997 family protein. Compared with CmoDUF1997 amino acid sequences between the wf mutants and N87 plants, the critical amino acid mutations (early termination of amino acids) occurred in wf mutants, so CmoCh14G005820 was predicted as a potential candidate for controlling the white‐flower trait. RNA‐sequencing analysis revealed that the expression of CmoCh14G005820 and most genes involved in carotenoid biosynthesis was significantly downregulated in wf mutants, whereas the expression of several genes responsible for carotenoid degradation was upregulated in wf mutants. This finding suggested that carotenoid metabolism may participate in the formation of flower colour in pumpkin. Overall, our results provided a theoretical basis for understanding the genetic mechanisms underlying white‐flower formation in pumpkin. [ABSTRACT FROM AUTHOR]

Soil and water salinity is an important limiting factor affecting yield and production levels in arid and semi-arid areas. Salt tolerance during germination is an important parameter that also affects the other plant development stages. In this respect, this study was designed to determine the responses of pumpkin seed varieties (Develi, Ürgüp, Hybrid) to different NaCl salinities. The study was carried out in 2022 in the laboratory of Biosystems Engineering Department of Erciyes University in randomized plots design with 3 replications. Experiments were conducted with 5 different water salinity. Germination percentage (GP), germination index (GI), mean germination time (MGT), seedling vigor index (SVI), ion leakage (Il), radicula length (RL) and plumule length (PL), root and shoot fresh and dry weights and some mineral composition (Na, K, Ca) were examined. Proline, antioxidant capacity, total phenolic and DPPH content were significantly affected by salinity. In scatter plot correlation analysis SVI a positive correlation was observed between GP (r2 = 0.774), GI (r2 = 0.745), RL (r2 = 0.929), FRW (r2 = 0.837), FSW (r2 = 0.836), DRW (r2 = 0.894), AC (r2 = 0.747), TP (r2 = 0.640) and DPPH (r2 = 0.635). It was determined that there were negative correlations between SVI and MGT (r2 = − 0.902), II (r2 = − 0.588), DSW (r2 = − 0.682) and PR (r2 = − 0.344). Present findings revealed that investigated parameters were significantly affected by increasing salinity levels. While Hybrid cultivar was the most affected by salinity, Develi cultivar was found to be resistant to saline conditions. [ABSTRACT FROM AUTHOR]

Boris Timofeevich Matienko (1929-2004) was a respected plant anatomist and physiologist in Soviet Moldova. Matienko's research was primarily on the growth, development, and senescence of cucurbits, as well as apples, plums, and other vegetable crops. His studies provided a wealth of information to those interested in the anatomic structure of pumpkins, squash, gourds, watermelon, and minor Cucurbitaceae. Using insightful and detailed descriptions, he depicted cellular changes of the fruit during development, aging, and senescence. Practical experiments on storage corroborated his detailed microscope studies. Matienko worked during a time when the problems of the agriculture sector in Moldova were immense and varied, and he faced structural and societal challenges that underscore his scientific accomplishments. The quality of cucurbits grown in Moldova and the region improved as a result of this research. He was widely respected by colleagues and the scientific community, and his work continues to provide insight to researchers of these important crops. [ABSTRACT FROM AUTHOR]

The recent release of 2,4-D- and dicamba-tolerant soybean traits has increased the risk of off-target herbicide injury and yield loss for specialty crop growers in the midwestern United States. Most dicotyledonous plants, including many specialty crops like pumpkin (Cucurbita pepo), are susceptible to synthetic auxin herbicides; however, the relationship between off-target herbicide rate, visible crop injury, and eventual yield loss is not well documented. The objective of this 2-year field study in 2019 and 2020 was to determine the effect of sublethal herbicide rates of 2,4-D and dicamba on visible injury and crop yield loss in pumpkin when applied at the vegetative and flowering growth stages. Herbicides included 2,4-D choline salt (1066 g ae·ha21 labeled rate) and dicamba diglycolamine salt (560 g ae·ha21 labeled rate) ranging from 1/500 to 1/4 of the labeled rate. Visible injury ratings were recorded every 7 d after application and pumpkins were harvested and weighed when ripe. Injury and yield data were fit to a four-parameter log-logistic regression model to estimate effective doses (ED) required for 5% to 50% visible injury or yield loss. Pumpkin treated with the 1/10 and 1/4 rates of 2,4-D at both growth stages had visible injury (± 1 SE) ranging from 8% (± 3%) to 55% (± 3%), but injury did not always result in yield loss. Maximum yield loss from 2,4-D was 32% (± 2%), observed following the 1/4 rate at the vegetative growth stage in 2020 (estimated ED for 20% yield loss was ~1/50). Pumpkin treated at the vegetative growth stage with the 1/10 and 1/4 rates of dicamba resulted in 65% (± 6%) to 82% (± 1%) visible injury and 33% (± 2%) to 86% (± 14%) yield loss (estimated ED for 20% yield loss was ~1/10 in 2019 and ~1/15 in 2020). At the flowering stage, dicamba rates of 1/10 and 1/4 caused visible injury of 31% (± 2%) to 74% (± 5%) and yield loss of 26% (± 10%) to 60% (± 14%) (estimated ED for 20% yield loss was ~1/20 in 2019 and ~1/5 in 2020). Susceptibility of pumpkin to 2,4-D and dicamba suggests herbicide applicators and pumpkin growers should consider strategies that mitigate off-target movement, including using nozzles that increase droplet size, shielded sprayers, thorough tank cleanout, buffer zones, and programs that facilitate communication between applicators and growers. [ABSTRACT FROM AUTHOR]

Background: Photoperiod, or the length of the day, has a significant impact on the flowering and sex differentiation of photoperiod-sensitive crops. The "miben" pumpkin (the main type of Cucurbita moschata Duch.) is well-known for its high yield and strong disease resistance. However, its cultivation has been limited due to its sensitivity to photoperiod. This sensitivity imposes challenges on its widespread cultivation and may result in suboptimal yields in regions with specific daylength conditions. As a consequence, efforts are being made to explore potential strategies or breeding techniques to enhance its adaptability to a broader range of photoperiods, thus unlocking its full cultivation potential and further promoting its valuable traits in agriculture. Results: This study aimed to identify photoperiod-insensitive germplasm exhibiting no difference in sex differentiation under different day-length conditions. The investigation involved a phenotypic analysis of photoperiod-sensitive (PPS) and photoperiod-insensitive (PPIS) pumpkin materials exposed to different day lengths, including long days (LDs) and short days (SDs). The results revealed that female flower differentiation was significantly inhibited in PPS_LD, while no differences were observed in the other three groups (PPS_SD, PPIS_LD, and PPIS_SD). Transcriptome analysis was carried out for these four groups to explore the main-effect genes of sex differentiation responsive to photoperiod. The main-effect gene subclusters were identified based on the principal component and hierarchical cluster analyses. Further, functional annotations and enrichment analysis revealed significant upregulation of photoreceptors (CmCRY1, F-box/kelch-repeat protein), circadian rhythm-related genes (CmGI, CmPRR9, etc.), and CONSTANS (CO) in PPS_LD. Conversely, a significant downregulation was observed in most Nuclear Factor Y (NF-Y) transcription factors. Regarding the gibberellic acid (GA) signal transduction pathway, positive regulators of GA signaling (CmSCL3, CmSCL13, and so forth) displayed higher expression levels, while the negative regulators of GA signaling, CmGAI, exhibited lower expression levels in PPS_LD. Notably, this effect was not observed in the synthetic pathway genes. Furthermore, genes associated with ethylene synthesis and signal transduction (CmACO3, CmACO1, CmERF118, CmERF118-like1,2, CmWIN1-like, and CmRAP2-7-like) showed significant downregulation. Conclusions: This study offered a crucial theoretical and genetic basis for understanding how photoperiod influences the mechanism of female flower differentiation in pumpkins. [ABSTRACT FROM AUTHOR]

This study aimed to analyze the mineral profiles, physicochemical and sensory properties of pumpkin dough chips (PDC), made from pumpkin pulp (Cucurbita moschata) and a combination of hazelnut and grain meals fried by oven (OF), microwave (CMWF) and deep frying (DF). The experiment was established by 36 samples, including formulations (F = 4), frying techniques (FT = 3) and replications (R = 3). The effect of F, FT and F × FT factors on the moisture, ash, protein, oil, hydroxymethylfurfural, phenolics, DPPH-RSA, TEAC, β-carotene, Hunter L*, a*, b*, browning index, hardness, fracturability and minerals of PDC were found significant statistically (P < 0.05). As a result of ICP-MS analysis of PDCs, K, Na, Ca, Mg, and Si minerals were higher than other minerals. While the OF chips had most appreciated, panellists found the DF chips oily. Also, there was a harmony between the pumpkin in the PDC and the cereal flours and hazelnuts. [ABSTRACT FROM AUTHOR]

Root and crown rot incited by an oomycete, Phytophthora melonis, causes significant yield losses in commercial pumpkin (Cucurbita pepo) production worldwide. Currently, resistant cultivars and knowledge of molecular mechanism of C. pepo against P. melonis are scarce. Here, we analysed the quantitative gene expression changes of 10 candidate gene markers (bHLH87, ERF014, HSF, MYB, PR-1, WRKY21, CPI, POD, PSK, SGT) in pumpkin roots and leaves at three time points (h post-inoculation, hpi) following inoculation with P. melonis in two resistant (Ghelyani and Tanbal), and two susceptible (Marmari and Khoreshti) varieties of pumpkin. Gene expression using quantitative real time PCR along a time course revealed the strongest transcriptomic response at 48 and 72 hpi in resistant genotypes, 1.1-2.7-fold in roots and leaves, respectively, with a high significant correlation (r = 0.857**-0.974**). We also found that CPI, PSK, SGT1 and POD act as a dual regulator that similarly modulate immunity not only against P. melonis, but also against other diseases such as early blight (Alternaria cucumerina), powdery mildew (Podosphaera xanthii), downy mildews (Pseudoperonospora cubensis), and pathogenic plant nematodes (Meloidogyne javanica). Furthermore, significantly higher activities of the ROS scavenging defence enzymes, catalase (1.6-fold increase) and peroxidase (6-fold increase) were observed in the roots of resistant cultivars at different hpi compared with non-inoculated controls. In addition, the biomass growth parameters including leaf and root length, stem and root diameter, root fresh weight and volume were significantly different among studied genotypes. Cumulatively, the transcriptome data provide novel insights into the response of pumpkins for improving pumpkin breeding to P. melonis. [ABSTRACT FROM AUTHOR]

Mass, size, and shape attributes are important for the design of planters, breeding studies, and quality assessment. In recent years, machinery design and system development studies have taken these factors into consideration. The aim of this study is to explore classification models for four pumpkin seed varieties according to their physical characteristics by machine learning. Binary classification is important because it ensures that the quality characteristics of the seeds are very similar to each other. The pumpkin seed varieties of Develi, Sena Hanım, Türkmen, and Mertbey were discriminated in pairs. Five machine learning algorithms (Naïve Bayes, NB; support vector machine, SVM; random forest, RF; multilayer perceptron, MLP; and kNN, k-nearest neighbors) were applied to assess the classification performance. In all pairs, the pumpkin seed varieties of Develi and Mertbey were discriminated with the highest accuracies of 85.00% for the MLP model and 84.50% for the SVM model and 83.50% for the RF. In the MLP algorithm, TP rate reached to 0.790 for Develi and 0.910 for Mertbey, Precision to 0.898 for Develi and 0.813 for Mertbey, F-measure to 0.840 for Develi and 0.858 for Mertbey, PRC area to 0.894 for Develi and 0.896 for Mertbey, and ROC area to 0.907 for both varieties. Variety of pairs was followed by Sena Hanım and Türkmen (84.50%, MLP) and Türkmen and Mertbey (82.50%, SVM). For the selected input attributes, the highest mass (0.23 g), length (22.08 for Mertbey, 21.43 for Sena Hanım), and geometric mean diameter (8.79 mm) values were obtained from Sena Hanım variety, while shape index (3.40) from Mertbey variety. Multivariate statistical results showed that differences in attributes were significant (p < 0.01). Wilks' lambda statistics found that the portion of the unexplained difference between groups was 46.60%. Develi and Sena Hanım varieties with the lowest Mahalanobis distance values had similar characteristics. Present results revealed that SVM and MLP may be used effectively and objectively for the classification of pumpkin seed varieties. [ABSTRACT FROM AUTHOR]

Late flowering is a serious bottleneck in pumpkin (Cucurbita moschata Duch.) agriculture production. Although key genes governing flowering time have been reported in many species, the regulatory network of flowering in pumpkin remains largely obscure, thereby impeding the resolution of industry‐wide challenges associated with delayed fruit ripening in pumpkin cultivation. Here, we report an early flowering pumpkin germplasm accession (LXX‐4). Using LXX‐4 and a late flowering germplasm accession (HYM‐9), we constructed an F2 segregation population. A significant difference in FLOWERING LOCUS T‐LIKE 2 (FTL2) expression level was identified to be the causal factor of the flowering time trait discrepancy in LXX‐4 and HYM‐9. Moreover, we have shown that a 21 bp InDel in the FTL2 promoter was the key reason for the waxing and waning of its transcript level. The 21 bp deletion excluded a repressor‐AGL19 and recruited activators‐BBX7, WRKY40 and SVP to the FTL2 promoter in LXX‐4. Together, our data add a useful element to our knowledge which could be used to simplify breeding efforts for early‐maturing pumpkin. Summary statement: An InDel in FTL2 promoter exclude repressor AGL19 and recruit activators BBX7, WRKY40 and SVP to the promoter, leading to increase of FTL2 transcript level and early floral initiation under short‐day conditions. [ABSTRACT FROM AUTHOR]

This paper presents research findings on the pelleting process of pine sawdust using bakery waste in a pelletizer. The addition of bakery waste (white wheat–rye bread, whole-grain rye bread, and pumpkin bread) to pine sawdust had a beneficial effect on the kinetic strength of the pellets obtained, an increase of up to approximately three percentage points. The density of pellets with the addition of bakery waste also increased, while the bulk density of the pellets decreased. The addition of bakery waste also had a positive effect on the power demand of the pelletizer. It was reduced from 3.08% (at a 10% addition of white wheat–rye bread) to 22.18% (at a 20% addition of pumpkin bread), compared to the process of compacting pure pine sawdust. In addition, all the pellets containing bakery waste had a lower energy yield (EY) determined based on lower heating value and energy inputs. This index was lower by 53 Wh·kg−1 for pine sawdust pellets with a 10% addition of pumpkin bread. The greatest reduction, on the other hand, was by 173 Wh·kg−1 for pellets, with a 20% addition of white wheat–rye bread. In each case, an increase in the share of bakery additives resulted in a decrease in the energy yield from the pellets obtained. The smallest reduction in EY was found when pumpkin bread was used as an additive (from 53 to 133 Wh·kg−1). Considering all the parameters analyzed characterizing the pellets obtained, it was concluded that the addition of bakery residues to pelletized pine sawdust should not exceed 10%. Further increases in the proportion of bakery waste did not yield relative benefits, due to the deterioration of the energy characteristics of the pellets obtained. [ABSTRACT FROM AUTHOR]

In this study, the characteristic properties of pumpkin pulp flour (PPF) obtained from the waste of the pumpkin seed production were determined. The pulp parts of the de-seeded and peeled pumpkins were used for this purpose. Proximate composition, total dietary fiber (TDF), insoluble dietary fiber (IDF), soluble dietary fiber (SDF), mineral content, amino acid content, ß-carotene content, pH, color, glass transition temperature (Tg), water holding capacity (WHC), oil holding capacity (OHC), swelling capacity (SC), emulsion stability (ES) and emulsion activity (EA) values of PPF were determined. PPF had a low lipid content (0.72±0.06%) and a high ash content (8.34±0.19%). The pH, Tg, TDF, IDF, SDF, WHC, OHC and SC values are 5.61±0.01, 19.19±1.86°C, 26.13±0.17%, 19.82±0%, 6.31%±0.35, 12.91±0.40 g/g, 3.74±0.10 g/g, 12.48±0.57 mL/g, respectively. PPF were a rich source of glutamic acid, glycine, and aspartic acid, and contains high levels of potassium among major elements, and iron among minor elements. [ABSTRACT FROM AUTHOR]

This study intends to explore the effects of Cucurbitacin B (CuB) and KIF20A on esophageal carcinoma (ESCA). Data were downloaded from the Cancer Genome Atlas (TCGA) database. The expression properties of KIF20A have been confirmed by GEPIA and ualcan from TCGA. The expression of KIF20A was determined using western blotting in ECA109 and KYSE150 cells after transfection with KIF20A, KIF20A siRNA, or numerical control siRNA (si-NC). Then, different concentrations of CuB were used to treat ECA109 and KYSE150 cells. CCK-8 and colony formation assays were used to measure cell viability, and a Transwell assay was utilized to assess cell migration and invasion ability. N-cadherin, E-cadherin, snail, p-Janus kinase 2 (JAK2), JAK2, p-signal transducer and activator of transcription 3 (STAT3), and STAT3 expression levels were evaluated using western blot. KIF20A was higher expressed in ESCA than in normal cells, and its overexpression was associated with squamous cell carcinoma, TNM stage, and lymph nodal metastasis of ESCA patients. In ECA109 and KYSE150 cells, increased KIF20A facilitated cell proliferation, migration, and invasion, whereas the knockdown of KIF20A can reverse these effects with N-cadherin. Snail expression diminished and E-cadherin increased. Similarly, CuB treatment could inhibit cell proliferation, migration, and invasion concentration dependently. Furthermore, KIF20A accelerated the expression of p-JAK2 and p-STAT3, while the application of CuB inhibited KIF20A expression and attenuated the activation of the JAK/STAT3 pathway. These findings revealed that CuB could inhibit the growth, migration, and invasion of ESCA through downregulating the KIF20A/JAK/STAT3 signaling pathway, and CuB could serve as an essential medicine for therapeutic intervention. [ABSTRACT FROM AUTHOR]

The effects of storage temperature on bioactive compounds, decay, and chilling injury development of pumpkins during cold storage and shelf-life periods were examined. In the present study, fruits were stored at 3°C, 8°C, and ambient temperature (control) for 180 days and, after that, fruits were stored at 20°C for 7 days to determine shelf-life performance. The lowest weight loss was found in the fruit stored at 3°C during cold storage while the highest flesh firmness was found on the fruit stored at 8°C. Total soluble solids, L-ascorbic acid and glucose contents were found to be higher in the fruit stored at 3°C compared to other storage temperatures. The highest total carotenoid content and the lowest decay incidence were recorded by the fruit stored at 3 and 8°C. Fruit stored at 3°C showed better quality parameters as compared to other temperatures under study. Although 3°C was better to protect the biochemical composition of pumpkin fruits, decay rate increased at this temperature due to chilling injury. It is concluded that the storage temperature of 8 °C was optimum to maintain fruit quality and biochemical contents and to inhibit decay during long-term storage of pumpkins. [ABSTRACT FROM AUTHOR]

Genetic engineering is commonly used to improve the agronomic traits of crops. However, genetic transformation in pumpkin remains a challenge. Conducting transformation trials, we accidentally created transgenic L1 periclinal chimeras in pumpkins.Using our modified Agrobacterium‐mediated transformation, we generated transgenic L1 periclinal chimeras which have high value in research on development of the meristem.Fluorescence observations of transformed L1 cells enabled us to reveal cell fates. These L1 cells can develop into stomata, epidermal hairs, seed coat, and epidermis of the root, stem, leaf, flower, and fruit. These periclinal chimeras can be propagated vegetatively with minimal risk of transgene flow.This study offers new perspectives on development of the meristem and a promising technique for creating transgenic periclinal chimeras in plants. [ABSTRACT FROM AUTHOR]

Pumpkins (Cucurbita sp.) are currently sold in retail commercial bins categorized based on fruit size. There are no standards for these fruit sizes, thus creating discrepancies across the industry. Furthermore, there is not a published partial budget analysis for pumpkin fruit yield based on plant area. An observational study was conducted to quantify and standardize the fruit sizes of pumpkins packed into commercial bins. These proposed standardized fruit sizes were then correlated to the expected fruit size and quantity of different plant areas to estimate the total commercial bin yield. Additionally, a partial budget analysis was conducted to calculate the greatest profit per hectare with the varying plant areas. Pumpkins from bins labeled medium, large, extra-large, and jumbo were hand-measured to determine the diameter, length, and weight. Based on a discriminate analysis, 20% of pumpkins were incorrectly sorted based on current practices. The proposed standard fruit diameters for each bin size are as follows: medium, 23.5 to 26.8 cm; large, 26.9 to 29.9 cm; extra-large, 30.0 to 33.6 cm; and jumbo, 33.7 to 35.5 cm. The results of a partial budget analysis showed that the most profitable plant spacing area is 0.9 m² with a 1.5-m row width, which will earn $37,163/ha. Profit for pumpkin production is contingent on both fruit quantity and fruit size because these factors dictate the quantity and category of commercial bins. Growers should consider bothmetrics to optimize their operation. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of replacing of rice flour with different ratios (0, 10, 20, and 20% w/w) of pumpkin powder on the rheology of batter and textural characteristics of gluten free rice cake. A factorial experiment with a completely randomized design with three replications was used for data analysis. The use of pumpkin powder in rice cake formulations significantly improved rheology of batter and textural characteristics of the samples (p< 0.05). By increasing the replacement level of rice flour with pumpkin powder to 30%, viscosity (from 682.66 to 3518.44 cp), hardness (from 21.11 to 53.97 g), cohesiveness (from 0.83 to 0.94), and springiness (from 9.74 to 13.49 mm) of batter cake improved significantly, but the adhesiveness (3.15 mJ) of batter increased compared to control (1.08 mJ) (p <0.05). However, the incorporation of pumpkin powder in the sample formulations up to 30%, increased the hardness (from 456.17 to 800.11 g), cohesiveness (from 0.56 to 0.67), springiness (from 8.68 to 11.43 mm), and chewiness (from 2231 to 6148 g) of the final cakes. Addition of pumpkin powder (up to 30%) in gluten free rice cake formulation caused the significant increasing of the total color differences (ΔE), browning index, and saturation index (chroma) in the cakes compared to control and moreover, increased the hue angle to 90owhich indicated an increase in the yellowness of the samples containing higher the replacement level of rice flour with pumpkin powder. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to clarify the effects of different degradation films on the growth of pumpkin and the characteristics of the changes in the degradation performance and mechanical properties of the films.【Method】We investigated the trends of mechanical indexes such as film elongation and breaking force values of black PE film (CK), white degradable film (T1), black degradable film (T2) and black carbon dioxide-based degradable film (T3) and their effects on the growth of pumpkin plants during the reproductive period of pumpkin through the field mulching test.【Result】With the increase of coating time, it was found that the mechanical properties of different membranes showed a decreasing trend through the mechanical properties testing and degradation degree analysis during the important fertility period, and the values of the mechanical indexes of the three degradable membranes showed the most obvious decreasing magnitude. Among them, the initial values of all mechanical indexes of T1 treated membranes were higher than those of T2 and T3, and the degradation degree was weaker than that of T2 and T3 during the reproductive period of pumpkin; the values of all mechanical characteristics of T2 and T3 were relatively similar, and the degradation degree of the membranes was of the same grade. Through correlation analysis, it was concluded that the mechanical characteristics of the degraded film were negatively correlated with the degree of degradation, and the degree of degradation increased with the decrease of the mechanical characteristic values; the SPAD value, chlorophyll a and b contents of the leaves of CK mulching treated plants were higher than those of the three degraded films, and they had stronger photosynthetic capacity, so the growth and development in the number of leaves, area and length of the vine showed better performance. Black degradation film (T2) and black carbon dioxide-based degradation film (T3) treated pumpkins had increased soluble solids and Vc contents, which had an enhancement effect on the overall quality of pumpkin, and the pumpkin yields of the two converged and were not significantly different from those of CK. 【Conclusion】 Degradation of the mechanical properties of the degradable film will accelerate the rate of degradation and affect the performance of film-assisted production, covering the black degradable film and black carbon dioxide-based degradable film can be made to improve the quality of pumpkin quality, and pumpkin yield is not significantly different from the covering of black PE film. In view of the environmentally friendly nature of degradable films in agricultural production, it is feasible to replace ordinary PE films in crop and vegetable production. [ABSTRACT FROM AUTHOR]

Frugivorous insects utilize both olfactory and visual cues to locate their host plants. Although volatiles have been extensively studied for detecting infested fruits, the role of visual cues in oviposition site selection remains mostly unknown among frugivorous insects. To investigate physiological changes in a host plant, we measured the quantum yield and reflectance of three wavelengths of light (350, 450 and 520 nm) after puncturing the surfaces of commercially grown pumpkins using insect pins during two different seasons outdoors. Quantum yields did not show significant differences between undamaged and simulated oviposition sites. Two wavelengths within the visual spectrum were similar between the two treatments. However, photon counts at 350 nm, in the ultraviolet range, were 1.76 fold higher in simulated oviposition sites than in undamaged sites, which was consistently observed across three field seasons. Considering that frugivorous insects and other phytophagous insects recognize the 350 nm wavelength for host identification and oviposition, we conclude that the disparities in ultraviolet reflectance can serve as a baseline for assessing the actual oviposition response of fruit flies. This finding contributes to the role of visual cues in the oviposition behavior of frugivorous insects and the development of a nondestructive detection method for pumpkin fruit flies. [ABSTRACT FROM AUTHOR]

The processing methods, especially cooking, can cause quality losses, particularly in the nutritional value of the fresh product. This study investigated the effects of preprocessing methods on the nutritional properties of pumpkin and the physicochemical and sensory properties of pumpkin yogurt. Two different pumpkin varieties (Cucurbita pepo and Cucurbita maxima) were subjected to three different preprocessing methods (freeze‐drying, boiling, and baking). Boiling significantly increased antioxidant activity (p ≤.05), followed by baking. C. maxima had higher TDF and TPC than C. pepo, but in both pumpkin varieties, TDF did not change with heat treatment (boiling and baking), while TPC decreased. Mineral contents remained the same or decreased with heat treatment, except for Mn and Fe. In particular, the addition of C. maxima significantly affected the color parameters (L*, a*, b*) of yogurt and improved WHC (from 68.9% to 91.6%) and hardness (from 58.0 to 193.5 g; p ≤.05). The sensory evaluation concluded that heat‐treated (boiled and baked) samples were preferred more than freeze‐dried raw ones. In conclusion, the results revealed that adding boiled and baked pumpkins, especially the preference for C. maxima instead of C. pepo, improved the quality parameters of yogurt. [ABSTRACT FROM AUTHOR]

Pumpkins are increasingly regarded as functional foods since they contain valuable nutrients and bioactive substances that have positive health effects. The number of bioactive components depends on the origins and parts of the pumpkin, which define its antioxidant capacity. This study evaluated the total phenolic, total flavonoid, and ascorbic acid content and antioxidant activities of three parts of pumpkin from four different sample origins and investigated the correlation between them. The content of bioactive components in pumpkin from Dukem, Ethiopia, was highest in the flesh, peel, and seeds compared to the other sample areas. The Pearson correlation result indicated a strong relationship between the total phenolic, flavonoid, and ascorbic acid contents of pumpkin and their respective antioxidant activities. This study found that the total phenolic, total flavonoid, and ascorbic acid contents and antioxidant activity varied across the pumpkin sample sources and pumpkin parts. [ABSTRACT FROM AUTHOR]

This study aimed to assess the nutritional properties of dietary lipids obtained through the modification of aqueous enzymatically extracted pumpkin seed (Cucurbita pepo. L) oil. The optimal growth conditions for producing pectinase using strain Aspergillus sp. 391 were determined, and partial characterization of pectinase and commercial cellulase was conducted. The enzymatic extraction was performed at pH 4.0, 50 °C, for 24 h, using a combination of pectinase and cellulase for optimum effectiveness. The crude oil obtained was analyzed for acid, peroxide, and fatty acid composition. The study found a high amount of unsaturated fatty acids, mainly linoleic acid (C18:2), and a 59% oil recovery rate. Subsequently, this oil was subjected to enzymatic acidolysis with capric acid in solvent-free media, catalyzed by lipase Lipozyme RM IM®, resulting in a product with a higher incorporation degree (48.39 ± 0.5 mol%), observed after 24 h at 60 °C using molar ratio oil:acid capric of 1:9 (run 4). The nutritional properties of this oil were improved. [ABSTRACT FROM AUTHOR]

Summary: Jaggery, a natural unrefined sweetener rich in phytonutrients, could be used as a healthier substitute to develop functional foods. The present study intended to utilise liquid jaggery in place of refined sugars to develop a functional fruit snack bar. Five different formulations were prepared by varying levels (0%–100%) of liquid jaggery in conventional fruit bar containing pumpkin pulp as base material. The addition of jaggery significantly enhanced bioactive constituents and minerals, which were also confirmed by ICAP analysis. Based on sensory characteristics, pumpkin bars prepared by replacing sugar with 50% liquid jaggery were selected for shelf study evaluation. Bars were packed in laminated foil and butter paper and stored under room (25 ± 1 °C) and refrigerated (4 ± 1 °C) conditions. Irrespective of storage temperature and packaging materials, significant variation was noticed in moisture content and textural characteristics of stored fruit bars. Total and reducing sugars and titratable acidity increased while bioactive constituents and colour values remarkably decreased with progression of storage period. Except moisture, the rate of loss of quality attributes was rapid at room temperature. Stored jaggery‐based pumpkin bars packed in both packagings showed satisfactory microbial and organoleptic quality for 6 months. [ABSTRACT FROM AUTHOR]

Pumpkins (Cucurbita sp.) grown in North Carolina are a nascent specialty crop that has only risen to a national production level in the past 10 years. There are only general cultural management guidelines for this region, resulting in variation in plant density and inefficient production. Production field studies of the cultivar Kratos were conducted to investigate the impact of plant density and row width on marketable yield and individual fruit size for large carving pumpkins. Plant densities of 2691, 3588, 5382, and 10,764 plants per hectare with row widths of 1.5 and 3.0 m were grown in 2020 and 2021 in North Carolina. Data regarding fruit size, fruit size variance, and yield per area were collected. Fruit size in terms of weight, length, and diameter increased as plant density decreased. There was no difference in fruit size variation between plant densities and row widths. The fruit number per hectare and fruit weight per hectare increased as plant density increased, with the highest production at 10,764 plants per hectare. For years combined, reducing the row width from 3.0 to 1.5 m increased the fruit weight and diameter, but not the length. Additionally, the 1.5-m row width produced more fruit weight per hectare than the 3.0-m row width for both years. Growers can optimize fruit weight per area and fruit number per area by using a density of 10,764 plants per hectare. Overall, using a row width distance that is more equidistant to the in-row spacing promotes higher fruit yield and larger fruit size. [ABSTRACT FROM AUTHOR]

The significant quantities of food waste that require disposal have a high environmental impact, and the depletion of non-renewable fuel sources has heightened the need to investigate sustainable and efficient methods of biomass conversion into energy. This research focuses on utilising pumpkin peel as a feedstock for energy recovery through microwave pyrolysis under different operating conditions. The study demonstrated that a higher biochar yield (11 wt%) was achieved at 0.9 kW. However, results revealed that superior quality biochar was obtained at 1.2 kW, characterized by high carbon content (70.33%), low oxygen content (23%), and significant pore formation in the carbon surface area. Optimal operating conditions, such as 1.2 kW, resulted in superior quality biochar and higher bio-oil generation. The pumpkin peel demonstrated the potential for CO2 (carbon dioxide) sequestration, with a rate of 14.29 g CO2 eq/kg. The research findings contribute to the exploration of sustainable solutions for biomass conversion and emphasize the importance of utilizing food waste for energy production while mitigating environmental impacts. [ABSTRACT FROM AUTHOR]

Vegetables are crucial for a healthy diet; however, when not washed properly, they can act as carriers of protozoa cysts and helminth eggs/larvae, posing a risk of transmission. The study aimed to determine the prevalence of parasitic contamination of fluted pumpkin promoted by inadequate handling conditions in Delta State, Nigeria. Samples of Telfairia occidentalis (fluted pumpkin vegetables) were collected from Effurun town and Otokutu local markets in washed and unwashed conditions and examined for parasitic contamination. An interviewer-administered questionnaire was used to obtain information on the socio-economic factors associated with parasitic contaminations on vegetables. The vegetable samples were examined using the simple sedimentation concentration method. The educational status of the vendors was ascertained, and 75% of the vendors had secondary school education, while 25% had primary school education. Four hundred seventyeight parasitic contaminants were recovered from the 48 vegetables samples. The species and stages of parasites detected were Trichuris spp, Moniezia spp, Enterobius vermicularis, Ascaris lumbricoides, Strongyloides spp, Hookworm eggs, Strongyle eggs Strongyloides stercoralis larvae, Schistostoma spp, Platynosomum fastosum and Eimeria spp. Fifty per cent (50%) of the produce was not washed, while 50% of the produce was washed before displaying by the retailers. The result from the paired t-test revealed a significant difference (p<0.05) in total parasitic contamination between washed and unwashed vegetable samples from both Effurun and Otokutu markets. This study showed that handling conditions of washing and the environment where vegetables are sold can influence the amount of parasitic load. Therefore, adequate washing and proper handling of raw edible vegetables before consumption are recommended. The government must create awareness and educate vegetable vendors about the importance of proper handling and hygiene, as they can play a major role in mitigating public health issues. [ABSTRACT FROM AUTHOR]

To solve the delamination & precipitation phenomena and to improve the quality of the spray-dried pumpkin powder (PP), the effects of adding mulberry leaf powder (MLP) and oat bran powder (OBP) on the PP quality were investigated. Results showed that POBP (PP + OBP, w:w = 2:1) had lower hygroscopicity (≤42.96 %), which was more homogeneous and stickiness after being brewed, effectively alleviating delamination & precipitation phenomena. Besides, PMLP (PP + MLP, w:w = 2:1) had the highest total phenolic content and total flavonoid content, with the values of 96.92 and 90.50 mg/100 g, respectively. PMLP also exhibited about ten times the antioxidant activity of PP. Furthermore, nine regression equations predicted the antioxidant properties of powders based on soluble solid values and bitterness. Overall, this study indicated that the addition of OBP and MLP improved the stability and nutritional quality of PP, respectively. [ABSTRACT FROM AUTHOR]

China is the leading country for pumpkin production in the world. As other cucurbits, diseases caused by viruses are among the serious threats to pumpkin production, but our knowledge on the virus species infecting pumpkin plants is fragmentary. To understand the occurrence of viral diseases on pumpkin, we determined the geographical distribution characteristics, relative abundance and evolutionary relationship of pumpkin infected viruses by meta-transcriptome sequencing (RNA-seq) and viromic analysis of 159 samples exhibited typical viral symptoms collected across China in this study. Totally, 11 known and 3 new viruses were identified. Interestingly, 3 new viruses identified in this study should be positive-sense single-stranded RNA virus whose hosts are prokaryotes. The viruses identified in different sampling locations exhibit significant variations in term of virus species and relative abundance. Here, the results provide valuable information for understanding the virus species and their diversity in cultivated pumpkin across major growing regions of China. • Significant variations through virus species and relative abundance from different regions. • Mixed viral infection are common in almost tested samples. • Expect the known viruses, 3 new viruses infecting prokaryotes were identified in them. [ABSTRACT FROM AUTHOR]

This study aims to analyze the effect of pumpkin flour substitution on consumer acceptance in making putu bamboo cake. This research was conducted at the Food Processing Laboratory, Catering Study Program, Faculty of Engineering, State University of Jakarta. The method used in this research is an experiment by substituting pumpkin flour in making putu bamboo cake by 15 grams, 20 grams and 25 grams against consumer acceptance. This study was assessed using the hedonic test to 30 moderately trained panelists consisting of students of the Catering Education Study Program who have passed the traditional cake course. The assessment included several aspects, namely color, taste, pumpkin aroma, pandan aroma, soft texture and porous texture. In processing the data from the panelists' assessment results using the Friedman test at a significant level α = 0.05. The results of hypothesis testing with the Friedman test showed that there was no significant effect on the substitution of pumpkin flour in making putu bamboo cake on consumer acceptance. Based on the results of the research conducted, pumpkin flour substitution of 20 grams is recommended in making putu bamboo cake. [ABSTRACT FROM AUTHOR]

Pumpkin, a member of the Cucurbitaceae plant family is an underutilized crop in Nigeria. This plant requires minimal agronomic input to produce optimal yield and it is drought tolerant. However, due to preservation, the fruit does not remain biochemically the same. What happens to squash stored for months after harvesting at room temperature needs to be examined from nutritional point of view. Therefore, this study was performed to evaluate the effects of storage at ambient temperature and relative humidity on selected vitamins (A, C, and E), and the proximate (crude protein, crude fiber, crude ash, and carbohydrate) in the green and orange morphotypes of pumpkin fruit. Pumpkins harvested in 2015 were stored for 4 months (January to April) and nutritional parameters evaluated monthly using standard assays. The results showed that the vitamin A content did not change significantly (P<0.05) over the storage period and morphotypes while the vitamin C and vitamin E contents decreased by 15% and 18%, respectively, when comparing the 1st and 4th months of storage. Crude protein and fat content decreased significantly over the storage period. However, crude fiber and ash increased in storage time. Except for crude fiber, the two morphotypes behaved similarly over the storage period. Remarkably, vitamin A concentrations in squash did not change after harvest, and some of the nutrients studied were not significantly affected during the first 4 months of storage. This information is especially important for people in rural areas limited in modern ways of storing fruit vegetable after harvest. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the levels of major (Na, Mg, K, Ca) and heavy (Cr, Mn, Fe, Ni, Cu, Zn, Cd) metals and health risk assessment of the peel, flesh and seed of pumpkins cultivated in Ethiopia, using microwave plasma-atomic emission spectrometry. Dried powdered samples (0.5 g) were wet digested with 69% HNO3 and 30% H2O2 in microwave digester. The percent recovery for the three parts of pumpkin samples were 90-110%. The mean concentration of metals in pumpkin peel, flesh, seed samples, respectively, were K (27806±9767, 29531±2452, 10667±1133), Ca (5267±2092, 3190±900, 2919±397), Mg (4716±469, 848±349, 4668±413), Fe (158±29, 111±46, 225±56), Na (167±116, 63.1±15, 171±123), Mn (28.9±1.4, 9.61±3.2, 33.4±4.9), Zn (24.2±4.4, 10.6±4.0, 67.5±12.3), Cu (8.14±1.1, 15.1±9.3, 10.8±0.9), Ni (3.79±2.4, 2.14±1.3, 3.44±2.8), Cr (0.28±0.1, 0.36±0.2, 0.69±0.1), Cd (0.24±0.1, 0.46±0.1, 1.39±0.2) mg/kg. The hazard index (HI) of pumpkin flesh and seed indicated ingestion of pumpkin is safe from non-carcinogenic risk. The total cancer risk (CR) due to consumption of pumpkin flesh and seed through Cr and Cd from the three sampling sites are in the range 1.1 x 10-4 -1.8 x 10-4, indicating a non-risk of exposure to cancer due to the consumption of pumpkin grown in the sampling areas. [ABSTRACT FROM AUTHOR]

Pumpkin (Cucurbita maxima) seed oil was extracted using petroleum ether and analyzed for fatty acid compositions and physicochemical parameters of the oil. The seeds were found to contain 43.6% (%w/w) oil. Linoleic (50.7%), oleic (18.8%), palmitic (17.9%) and stearic (12.4%) were found as the primary fatty acids in pumpkin seed oil. Physicochemical parameters of pumpkin seed oil were found to be acid value (1.32 mg KOH/g oil), saponification value (191 mg KOH/g oil), iodine value (114 g I2/100 g), peroxide value (3.6 meq/kg), specific gravity (0.91 g/mL), refractive index (1.47) and viscosity (24.7). According to the study's findings, 50% of the oil contains the essential fatty acid omega-6 (linoleic acid). The high concentration of essential linoleic acid in pumpkin seed oil implies that it is a nutrient-dense food. The physicochemical study of the pumpkin seed oil samples also showed that they were suitable as industrial ingredients for making soap, cosmetics, medications, and food additives, among other things. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of replacing of rice flour with different ratio (0, 10, 20, and 20 % w/w) of pumpkin powder on the physicochemical and sensory characteristics of gluten free rice cake. Wheat cake were made using the same method used to make the rice flour with different ratio (0, 10, 20, and 20 % w/w) of pumpkin powder, as the control samples. A factorial experiment with a completely randomized design with three replications was used for data analysis. The use of pumpkin powder in rice and wheat cake formulations significantly improved the technological characteristics and sensory attributes of the samples (p< 0.05). As the replacement level of rice and wheat flour with pumpkin powder increased (p <0.05), the viscosity of rice cake and wheat cake batter increased from 682.66 cp (control sample) to 3518.44 cp (% 30 pumpkin powder) and from 699.50 cp (control sample) to 4036.92 cp (% 30 pumpkin powder), respectively (p< 0.05). As the replacement level of rice and wheat flour with pumpkin powder increased, the volume and porosity of the cake were decreased and the hardness, fiber, ash, and the lightness of them were increased (p <0.05). The rice and wheat cakes containing the % 20 of pumpkin powder received the highest sensory scores (color, taste, odor, texture, and overall), as compared to control (p <0.05). Addition of pumpkin powder in gluten free rice cake formulation compared with wheat cake formulation improved the lightness and decreased the redness and yellowness of the samples that was acceptable to customers. These achievements will pave the way for using the combination technologies involving the mixing the pumpkin powder and rice flour for improving physicochemical and sensory characteristics of gluten free cakes. [ABSTRACT FROM AUTHOR]