1. FXR agonist obeticholic acid induces liver growth but exacerbates biliary injury in rats with obstructive cholestasis
- Author
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Michal Heger, Lindy K. Alles, Dirk R. de Waart, Megan J. Reiniers, Bulent Ergin, Adrie Maas, Thomas M. van Gulik, Pim B. Olthof, Steven W.M. Olde Damink, Frank G. Schaap, Joanne Verheij, Rowan F. van Golen, Lianne R. de Haan, Daniël A. Lionarons, Peter L.M. Jansen, Zehra Uz, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Graduate School, Surgery, Tytgat Institute for Liver and Intestinal Research, ACS - Atherosclerosis & ischemic syndromes, AGEM - Endocrinology, metabolism and nutrition, AGEM - Re-generation and cancer of the digestive system, ACS - Microcirculation, Translational Physiology, Medical Biology, Pathology, RS: FHML MaCSBio, RS: NUTRIM - R2 - Liver and digestive health, MUMC+: MA Maag Darm Lever (9), and RS: NUTRIM - R2 - Gut-liver homeostasis
- Subjects
0301 basic medicine ,Male ,DRAINAGE ,Administration, Oral ,lcsh:Medicine ,PROTECTS ,chemistry.chemical_compound ,0302 clinical medicine ,Cyclin D1 ,lcsh:Science ,ATP Binding Cassette Transporter, Subfamily B, Member 11 ,Liver injury ,Multidisciplinary ,Cholestasis ,Bile acid ,PROLIFERATION ,PARTIAL-HEPATECTOMY ,Obeticholic acid ,Organ Size ,G protein-coupled bile acid receptor ,Liver regeneration ,HEPATIC REGENERATION ,030211 gastroenterology & hepatology ,FARNESOID-X-RECEPTOR ,EXPRESSION ,medicine.medical_specialty ,medicine.drug_class ,Chenodeoxycholic Acid ,Article ,Biliary injury ,03 medical and health sciences ,Internal medicine ,medicine ,STEATOSIS ,Animals ,cdc25 Phosphatases ,business.industry ,lcsh:R ,medicine.disease ,eye diseases ,Liver Regeneration ,Rats ,Fibroblast Growth Factors ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,chemistry ,Gene Expression Regulation ,PORTAL-VEIN EMBOLIZATION ,Farnesoid X receptor ,lcsh:Q ,business ,BILE-ACID - Abstract
Cholestasis impairs liver regeneration following partial liver resection (PHx). Bile acid receptor farnesoid X-receptor (FXR) is a key mediator of liver regeneration. The effects of FXR agonist obeticholic acid (OCA) on liver (re)growth were therefore studied in cholestatic rats. Animals underwent sham surgery or reversible bile duct ligation (rBDL). PHx with concurrent internal biliary drainage was performed 7 days after rBDL. Animals were untreated or received OCA (10 mg/kg/day) per oral gavage from rBDL until sacrifice. After 7 days of OCA treatment, dry liver weight increased in the rBDL + OCA group, indicating OCA-mediated liver growth. Enhanced proliferation in the rBDL + OCA group prior to PHx concurred with a rise in Ki67-positive hepatocytes, elevated hepatic Ccnd1 and Cdc25b expression, and an induction of intestinal fibroblast growth factor 15 expression. Liver regrowth after PHx was initially stagnant in the rBDL + OCA group, possibly due to hepatomegaly prior to PHx. OCA increased hepatobiliary injury markers during BDL, which was accompanied by upregulation of the bile salt export pump. There were no differences in histological liver injury. In conclusion, OCA induces liver growth in cholestatic rats prior to PHx but exacerbates biliary injury during cholestasis, likely by forced pumping of bile acids into an obstructed biliary tree.
- Published
- 2018