1. Differential expression of genes related to HFE and iron status in mouse duodenal epithelium
- Author
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Annabelle Monnier, Bertrand Toutain, Marc Aubry, Stéphanie Mottier, Patricia Fergelot, Emmanuelle Abgueguen, Hélène Bédrine, Seiamak Bahram, Céline Chicault, Hélène Jouan, Jean Mosser, Magali Orhant, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Ouest Genopole, Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), and Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1)
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Iron Overload ,Duodenum ,Iron ,Biology ,digestive system ,Gene product ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology ,Gene expression ,Genetics ,medicine ,Animals ,Intestinal Mucosa ,Hemochromatosis Protein ,Gene ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,Transferrin saturation ,Gene Expression Profiling ,Histocompatibility Antigens Class I ,digestive, oral, and skin physiology ,Membrane Proteins ,nutritional and metabolic diseases ,Molecular biology ,Epithelium ,medicine.anatomical_structure ,Liver ,030220 oncology & carcinogenesis ,Hereditary hemochromatosis ,Hemochromatosis - Abstract
Iron absorption, distribution, use, and storage are thought to be tightly regulated since altered iron stores may lead to cellular damage and disease. HFE, the hereditary hemochromatosis gene product, is expressed in the crypts of the duodenum, but the molecular mechanism by which it contributes to the inhibition of iron absorption is still unknown. In this study we aimed to identify transcriptional profiles in the duodenal epithelium of Hfe −/− mice. We used dedicated microarrays to compare gene expression among the duodenum of Hfe −/− mice, induced iron overload mice, and control mice. We found 151 differentially expressed genes and unknown sequences between Hfe −/− mice and normal littermates. Gene profiling revealed a gene subset more specific for Hfe inactivation. The functional annotation of upregulated genes highlighted that mucus production and cell maintenance may account for the influence of Hfe on epithelium integrity and luminal iron uptake.
- Published
- 2006