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Delayed sexual maturation through gonadotropin receptor vaccination in the rainbow trout Oncorhynchus mykiss.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2009 Nov-Dec; Vol. 164 (2-3), pp. 107-16. Date of Electronic Publication: 2009 May 23. - Publication Year :
- 2009
-
Abstract
- In fish, gonadotropin hormones FSH-GTH1 and LH-GTH2 are less specific for their cognate receptors than in mammals. The respective reproductive functions of fish LH and FSH are thus difficult to establish. We aimed to study the effect of specific antagonists of the two gonadotropin receptors on trout sexual maturation in both sexes by targeting specific regions of LH and FSH receptors, Lhr and Fshr. Filamentous phages displaying Lhr specific or Fshr specific decapeptides from the extracellular hormone binding domain were engineered. Recombinant phages were used as receptor-specific antagonistic vaccines. Male and female trouts were immunized with anti-LHR, anti-FSHR, anti-FSHR+LHR or adjuvant alone, through multiple injections over 8-24 weeks, starting at different stages of sexual maturation. The consequences of immunization on gonadal development were evaluated by determining gonad growth, by histological analysis of testis and ovaries at the end of the vaccination period and by measuring blood plasma sex steroids using radioimmunoassay. We show for the first time in fish that the anti-receptor vaccinations could have specific antagonistic effects on the development of the reproductive functions; while the anti-FSHR affected the sexual maturation of prepubertal males and delayed sperm production, the anti-LHR blocked vitellogenesis in females. In maturing males, the combined anti-FSHR+LHR vaccine inhibited spermatogenesis and affected steroidogenesis. In that case, the effects of the vaccine on spermatogenesis were transient and reversible when immunization was stopped. Such an immunological strategy to specifically and transiently inhibit a receptor provides a promising approach for discovering their specific functions; it could also lead to a new technology for controlling the onset of puberty in aquaculture species.
- Subjects :
- Amino Acid Sequence
Animals
Body Weight drug effects
Body Weight immunology
Contraception, Immunologic methods
Contraception, Immunologic veterinary
Female
Immunity, Humoral
Male
Molecular Sequence Data
Oncorhynchus mykiss growth & development
Receptors, Gonadotropin antagonists & inhibitors
Sequence Homology, Amino Acid
Sexual Maturation immunology
Time Factors
Vaccination methods
Vaccines, Contraceptive pharmacology
Antibodies pharmacology
Oncorhynchus mykiss immunology
Oncorhynchus mykiss physiology
Receptors, Gonadotropin immunology
Sexual Maturation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 164
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 19467235
- Full Text :
- https://doi.org/10.1016/j.ygcen.2009.05.012