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24 results on '"Ou, Dexin"'

20. Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus.

Author

Ou, Dexin, Ling, Na, Tong, Liaowang, Zhang, Danfeng, Shen, Yizhong, and Ye, Yingwang

Subjects
*CRONOBACTER, *PHENOTYPIC plasticity, *PROTEOMICS, *TRANSCRIPTION factors, *FOOD pathogens, *EFFECT of salt on plants
Abstract

Cronobacter malonaticus is a highly hyperosmotic‐resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum‐sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and ΔluxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe‐S cluster proteins and oxidoreductases, suggesting a potential cross‐tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS‐mediated osmotic tolerance to controlling C. malonaticus contamination. [ABSTRACT FROM AUTHOR]

Published
2023
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24. Transcriptomic and biochemical analyses reveal the antifungal mechanism of cell-free supernatant from Aspergillus luchuensisYZ-1 against Aspergillus flavus

Author

Zhang, Xiyan, Jiao, Rui, Ren, Yuwei, Wang, Yang, Li, Hui, Ou, Dexin, Ling, Na, and Ye, Yingwang

Abstract

Aspergillus flavus(A. flavus) and its metabolites, aflatoxins, are widespread in crops and foods, threatening public health. Using chemical fungicides to control A. flavuscan pose safety hazards and cause environmental pollution. Therefore, there is an urgent need for safe and effective antifungal agents to control A. flavus. In this study, we isolated Aspergillus luchuensisYZ-1 cell-free supernatant (CFS), volatiles, and mycelial extracts to investigate their inhibitory effects on A. flavus. Results indicate that the CFS exhibited greater efficacy in impeding the growth and synthesis of aflatoxin B1 (AFB1) in A. flavus. Transcriptomic analysis revealed a significant downregulation of lipid metabolism, biosynthesis of other secondary metabolites, and glycan metabolism in A. flavustreated with CFS. Approximately 94% of the genes in the aflatoxin synthesis pathway were significantly downregulated. Furthermore, the CFS treatment significantly increased reactive oxygen species (ROS) production in A. flavus, affected its energy metabolism, and upregulated methane metabolism and the pentose phosphate pathway. We then investigated the inhibitory mechanism of YZ-1 against A. flavusby biochemical analysis. It was further confirmed that CFS had destructive effects in the cell wall and cell membrane of A. flavus, as indicated by leakage of nucleic acids, proteins, alkaline phosphatase (AKP), as well as decreased chitin and ergosterol content. In summary, this study provides novel insights into the mechanisms of CFS against A. flavus, suggesting its potential as a biocontrol agent in food and agriculture.

Published
2024
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