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This paper outlines the design and fabrication of a portable optomechatronic system based on the theta-2theta configuration, which explores various optical characterization techniques for transparent materials and dielectric thin films. These techniques include Brewster angle and Abelès-Brewster angle measurements, critical angle measurements, and the surface plasmon resonance technique. The system consists of a mechanical assembly of rotating stages, a semiconductor laser, a photodiode connected to a data acquisition card, and a user interface for controlling the stepper motor rotation stages. Utilizing a BK7 substrate, the motorized stage achieved a resolution of 0.010. The Brewster angle measured was 56.550, with a refractive index (n) of 1.5137. The relative error obtained was Δn/n = 3.82 × 10−4, with a sensitivity of 164.27 RIU/degree and an accuracy of 0.37/degree. Furthermore, the discrepancies between theoretical and experimental refractive indices for different prisms at 639 nm ranged from ±7 × 10−4 to ±73 × 10−4. After testing various samples, the system demonstrated its capability to perform fast, precise, and non-invasive measurements. Its portability allows for use in diverse environments and applications.

Methylene blue is a cationic organic dye commonly found in wastewater, groundwater, and surface water due to industrial discharge into the environment. This emerging pollutant is notably persistent and can pose risks to both human health and the environment. In this study, we developed a Surface Plasmon Resonance Biosensor employing a BK7 prism coated with 3 nm chromium and 50 nm of gold in the Kretschmann configuration, specifically for the detection of methylene blue. For the first time, laccases immobilized on a gold surface were utilized as bio-receptors for this organic dye. The enzyme was immobilized using carbodiimide bonds with EDC/NHS crosslinkers, allowing for the analysis of samples with minimal preparation. The method demonstrated validation with a limit of detection (LOD) of 4.61 mg L−1 and a limit of quantification (LOQ) of 15.37 mg L−1, a working range of 0–100 mg L−1, and an R2 value of 0.9614 during real-time analysis. A rainwater sample spiked with methylene blue yielded a recovery rate of 122.46 ± 4.41%. The biosensor maintained a stable signal over 17 cycles and remained effective for 30 days at room temperature.

The dynamic range of optical fiber refractive index sensors is mainly determined by the refractive index (RI) of the sensor surface in contact with the sample under test. In case of the refractive index sensor based on the hybrid fiber Fabry–Perot interferometer (HFFPI), the largest measurable refractive index value is equal to that of the fiber core. In this work, we demonstrate that it is possible to extend the refractive index dynamic range of a HFFPI by simply adjusting the optical path length (OPL) of the air and solid cavity to be equal or differ by just a small amount. Two isometric versions of the HFFPI (i-HFFPI) with a total length of 100 and 172 μm, where the OPL of the air and solid cavity are very similar, were fabricated and tested. The interferometers were immersed in different samples with a refractive index ranging from 1.000 to 1.733. The response of the interferometers was analyzed in the Fourier domain, and it was possible to establish a one-to-one relationship between the refractive index of the liquid sample and the amplitude of one of the peaks in the Fourier spectra. The amplitude of this peak experienced a linear increment when the RI of the surrounding medium was increased. Tracking the amplitude changes of a Fourier spectrum peak is straightforward which simplifies the online monitoring of the sensor. These features make this compact refractive index fiber sensor very appealing for biosensing applications.