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The objective of the present research was to diagnose and determine the frequency of protozoan and helminth species that affect the population of Lagoa do Vigário located in Campos dos Goytacazes, State of Rio de Janeiro, and to evaluate the physical-chemical and microbiological parameters of the waters of the lagoon. For that, 70 fecal samples were collected with the CoproPlus® collectors and the coproparasitological exams were performed according to the manufacturer's specifications. With the aid of a GPS device, water samples were collected from five points and physical-chemical and microbiological parameters were analyzed. Of the 70 people who participated in the study, 35% were infected by some parasite specie. The protozoa Endolimax nana, Giardia duodenalis, Entamoeba histolytica/dispar, Blastocystis hominis, Entamoeba coli and Iodamoeba butschlii were diagnosed, in addition to helminths such as Enterobius vermiculares and Strongyloides stercolaris. All water collection points showed contamination for total and thermotolerant coliforms with values greater than 2419.6 NMP / 100 mL. It is concluded that the results of this study demonstrate that the parasitic profile of the people who live around the lagoon is closely related to the precarious conditions of hygiene and education of individuals. The discharge of domestic effluent renders the water unsuitable for primary and secondary contact activities at some points analyzed. However, it is extremely important to apply Environmental Education projects to raise awareness and change the health habits of the population, guiding them in the search for a better quality of life. El objetivo de la presente investigación fue diagnosticar y determinar la frecuencia de especies de protozoos y helmintos que afectan a la población de la Lagoa do Vigário ubicada en Campos dos Goytacazes, Estado de Río de Janeiro, y evaluar los parámetros físico-químicos y microbiológicos de la aguas de la laguna. . Para ello se recolectaron 70 muestras de heces con colectores CoproPlus® y se realizaron los exámenes coproparasitológicos de acuerdo a las especificaciones del fabricante. Con la ayuda de un dispositivo GPS se tomaron muestras de agua de cinco puntos y se analizaron parámetros fisicoquímicos y microbiológicos. De las 70 personas que participaron en el estudio, el 35% estaba infectado por algún tipo de parásito. Se diagnosticaron los protozoos Endolimax nana, Giardia duodenalis, Entamoeba histolytica/dispar, Blastocystis hominis, Entamoeba coli e Iodamoeba butschlii, así como helmintos como Enterobius vermicularis y Strongyloides stercolaris. Todos los puntos de captación de agua presentaron contaminación por coliformes totales y termotolerantes con valores superiores a 2419,6 NMP/100 mL. Se concluye que los resultados de este estudio demuestran que el perfil parasitario de las personas que viven alrededor del lago está estrechamente relacionado con la precariedad de las condiciones de higiene y educación de los individuos. La descarga de efluentes domésticos hace que el agua no sea apta para actividades de contacto primario y secundario en algunos puntos analizados. Sin embargo, es de suma importancia implementar proyectos de Educación Ambiental para sensibilizar y cambiar los hábitos sanitarios de la población, orientándola en la búsqueda de una mejor calidad de vida. O objetivo da presente pesquisa foi diagnosticar e determinar a frequência de espécies de protozoários e helmintos que acometem a população da Lagoa do Vigário localizada em Campos dos Goytacazes, Estado do Rio de Janeiro, e avaliar os parâmetros físico-químicos e microbiológicos das águas da lagoa. Para isso, foram coletadas 70 amostras fecais com os coletores CoproPlus® e os exames coproparasitológicos foram realizados de acordo com as especificações do fabricante. Com o auxílio de um aparelho GPS as amostras de água foram coletadas de cinco pontos e foram analisados parâmetros físico-químicos e microbiológicos. Das 70 pessoas que participaram do estudo 35% estavam infectadas por alguma espécie de parasito. Foram diagnosticados os protozoários Endolimax nana, Giardia duodenalis, Entamoeba histolytica/dispar, Blastocystis hominis, Entamoeba coli e Iodamoeba butschlii, além de helmintos como Enterobius vermiculares e Strongyloides stercolaris. Todos os pontos de coleta de água apresentaram contaminação para coliformes totais e termotolerantes com valores maiores que 2419,6 NMP/100 mL. Conclui-se que os resultados deste estudo demonstram que o perfil parasitário das pessoas que moram ao redor da lagoa tem estreita relação com a precariedade das condições de higiene e educação dos indivíduos. O despejo de efluente doméstico torna a água imprópria para as atividades de contato primário e secundário em alguns pontos analisados. Contudo, é de suma importância a aplicação de projetos de Educação Ambiental para sensibilização e mudanças de hábitos sanitários da população, orientando-os na busca por melhor qualidade de vida.

Anaplasma phagocytophilum is an emergent hemoparasite in regions where ticks are regularly found. In order to investigate the rate of anti-A. phagocytophilum antibody in horses of the Mounted Police of Rio de Janeiro state and therefore the presence of this agent, 41 horses from the Cavalry Squadron (CS) and 50 from the Regiment of Mounted Police (RMP) were selected. For the serologic diagnosis the Indirect Fluorescent Antibody test was performed. In the present work, among 91 equine sera samples anti-A. phagocytophilum antibodies, with titer ≥ 1:80, were detected in 11 animals (12%), being five (5%) from males and six (7%) females, with no statistical difference. The adult animals with ages varying between five and 14 years presented the highest rate of positive reaction, although antibodies were detected in animals of all ages. We did not observe statistic differences in relation to the presence of anti-A. phagocytophilum antibodies among the animals bred in the CS and RMP. The presence of anti-A.phagocytophilim antibodies in horses of the Mounted Police with no clinical signs is indicative that the parasite is present in the enzootic form among the horses of the urban area. The circulation of the parasite among the animals is not dependent of the presence of tick infestations.

Anaplasma phagocytophilum is an emergent hemoparasite in regions where ticks are regularly found. In order to investigate the rate of anti-A. phagocytophilum antibody in horses of the Mounted Police of Rio de Janeiro state and therefore the presence of this agent, 41 horses from the Cavalry Squadron (CS) and 50 from the Regiment of Mounted Police (RMP) were selected. For the serologic diagnosis the Indirect Fluorescent Antibody test was performed. In the present work, among 91 equine sera samples anti-A. phagocytophilum antibodies, with titer ≥ 1:80, were detected in 11 animals (12%), being five (5%) from males and six (7%) females, with no statistical difference. The adult animals with ages varying between five and 14 years presented the highest rate of positive reaction, although antibodies were detected in animals of all ages. We did not observe statistic differences in relation to the presence of anti-A. phagocytophilum antibodies among the animals bred in the CS and RMP. The presence of anti-A.phagocytophilim antibodies in horses of the Mounted Police with no clinical signs is indicative that the parasite is present in the enzootic form among the horses of the urban area. The circulation of the parasite among the animals is not dependent of the presence of tick infestations. ResumoAnaplasma phagocytophilum é uma hemoparasitose emergente em regiões onde existem carrapatos. Com o objetivo de investigar a frequência de anticorpos anti-A. phagocytophilum em cavalos da Policia Montada do Estado do Rio de Janeiro e, consequentemente, os indícios da presença desse agente em áreas urbanas, foram selecionados 41 cavalos do Esquadrão de Cavalaria (EC) e 50 do Regimento de Polícia Montada (RPM). Para o diagnóstico sorológico, foi utilizado o método de Imunofluorescência Indireta. No presente trabalho, dentre 91 amostras de soro de equinos, foram detectados anticorpos anti-A. phagocytophilum com título ≥ 1:80 em 11 animais (12%), sendo que cinco (5%) eram machos e seis (7%) fêmeas, sem diferenças estatísticas. Os animais adultos com faixa etária entre cinco e 14 anos foram os que apresentaram maior frequência de reação positiva; no entanto, foram detectados anticorpos em animais de todas as faixas etárias. Não foram observadas diferenças estatísticas quanto à presença de anticorpos anti-A. phagocytophilum nos animais criados no EC e no RPM. A presença de anticorpos anti-A. phagocytophilum em cavalos da Policia Montada sem sinais clínicos aparentes é indicativo que A. phagocytophilum está presente de forma enzóotica na região urbana e a circulação do parasita entres os animais independe da presença de infestações aparentes de carrapatos nos animais.

The ratite group is composed of ostriches, rheas, emus, cassowaries and kiwis. Little research has been done on parasitism in these birds. The aim of this study was to determine the distribution of infections by gastrointestinal nematodes in ostriches in the state of Rio de Janeiro. For this, fecal samples were collected from 192 on 13 farms. From each sample, four grams of feces were used to determine the eggs per gram of feces (EPG) count, by means of the McMaster technique. Part of the feces sample was used for fecal cultures, to identify 100 larvae per sample. The results were subjected to descriptive central trend and dispersion analysis, using confidence intervals at the 5% error probability level in accordance with the Student t distribution, and Tukey’s test with a 95% confidence interval. The mean EPG in the state was 1,557, and the municipality of Três Rios had the lowest average (62). The city of Campos dos Goytacazes presented the highest mean EPG of all the municipalities analyzed. The northern region presented the highest mean EPG, followed by the southern, metropolitan, coastal lowland and central regions. Libyostrongylus species were observed on all the farms: L. douglassii predominated, followed by L. dentatus and Codiostomum struthionis. O grupo das ratitas é composto pelas avestruzes, emas, emús, cassuares e kiwi. São poucas as pesquisas sobre as parasitoses nessas aves. O objetivo deste estudo foi determinar a distribuição de infecções por nematóides gastrintestinais em avestruzes no Estado do Rio de Janeiro. Para tanto, foram coletadas amostras fecais de 192 avestruzes de 13 propriedades. De cada amostra, quatro gramas foram utilizados para a contagem de ovos por grama de fezes (OPG), pela da técnica de McMaster. Parte das fezes foi utilizada para cultivos fecais para identificação de 100 larvas por amostra. Os resultados foram submetidos à análise descritiva de tendência central e de dispersão, utilizando-se o intervalo de confiança ao nível de 5% de probabilidade de erro de acordo com a distribuição t de Student e teste de Tukey com intervalo de confiança de 95%. A média de OPG no Estado foi de 1.557, e o município de Três Rios obteve a menor média (62). A cidade de Campos dos Goytacazes teve a maior média de OPG de todos os municípios analisados. A Região Norte teve a maior média de OPG, seguida das regiões Sul, Metropolitana, Baixada Litorânea e Central. As espécies de Libyostrongylus foram observadas em todas as propriedades, predominando L. douglassii, seguido de L. dentatus e Codiostomum struthionis.

We aimed to evaluate the risk factor of serum reactive dogs to antileptospire agglutinin and their spatial distribution in an urban area. We collected 1,000 blood samples from dogs at 20 immunization centers, homogeneously distributed in the urban area (32 km²) of Botucatu, Sao Paulo, Brazil. Diagnosis was made by microscopic serum agglutination using 24 serovars of Leptospira spp. Statistical analysis was performed by Goodman's test with 5% of significance. Spatial clusters were tested by spatial scan statistic using SaTScan. We found that 17.9% dogs reacted to Leptospira spp. (p

Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2,0 x 10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. As alterações clínicas surgidas a partir do 3º dia após inoculação (DAI) foram: hipertermia, taquicardia, taquipnéia, anorexia, prostração, corrimento nasal e lacrimejamento. Estes sinais foram mais evidentes nos taurinos, espécie que apresentou, ainda, diarréia, fotofobia e conjuntivite. Foi possível isolar T. gondii da corrente sangüínea em todas as espécies. Nos taurinos, a partir do 5º DAI até o final do experimento, o parasito foi isolado de todas as amostras de sangue colhidas semanalmente, com exceção do 14º, 35º e 63º DAI. Os bubalinos apresentaram parasitemia no 7º, 14º, 35º e 70º DAI e os zebuínos apenas no 7º e 28º DAI, correspondendo aos picos de temperatura, em todas as espécies, sendo mais evidente em taurinos. Os parâmetros clínico-laboratoriais demonstraram que os taurinos foram mais sensíveis ao T. gondii do que os zebuínos e estes não diferiram significativamente dos bubalinos, que tiveram aparente normalidade clínico-laboratorial. From each of the host species (Bos indicus, Bos taurus and Bubalus bubalis) three individual animals were orally inoculated with 2.0 x 10(5) sporulated Toxoplasma gondii oocysts. Six other animals, being two from each of the host species, were kept as controls. Clinical abnormalities arising after the 3rd day post-infection were hyperthermia, tachycardia, tachypnea, anorexia, prostration, nasal flowing and lacrimation. These signs were conspicuous in the taurine hosts, which also presented diarrhea, photophobia and conjunctivitis. It was possible to isolate T. gondii from blood current from all species. In taurine, from 5th day after inoculation up to the final of the experiment, the parasite was isolated from all the samples of blood obtained weekly, with exception of 14th, 35th and 6rd day after inoculation. The buffalo cattle showed parasitemia at 7th, 14th, 35th and 70th day after inoculation and the zebu cattle only at 7th and 28th day after inoculation, corresponding to the peak of temperature, in all species, but more evident in taurines. The clinical-laboratorial parameters showed that the taurine was more sensible to T. gondii than the zebu cattle and these did not differ significatively from the buffalo cattle that had apparently clinical-laboratorial normality.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-29T13:53:42Z No. of bitstreams: 1 2016 - Lidiane Maria da Silva.pdf: 11158526 bytes, checksum: 92a272f4f09e476cee2262d286c61963 (MD5) Made available in DSpace on 2017-05-29T13:53:43Z (GMT). No. of bitstreams: 1 2016 - Lidiane Maria da Silva.pdf: 11158526 bytes, checksum: 92a272f4f09e476cee2262d286c61963 (MD5) Previous issue date: 2016-02-18 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES The thaminophilid passerines, just as other families of Passeriformes, can be parasitized by different species of coccidia, especially the genera Isospora Schneider, 1881 and Eimeria Schneider, 1875. In this context, this study aimed to identify, characterize and quantify coccidian species from Thamnophilidae in the Itatiaia National Park. Seven expeditions were performed at Itatiaia National Park, of which five were in conserved areas and two in areas around the park. A total of 184 species of birds were captured with mist nets, being 26 thaminophilid passerines. After fecal sampling and processing were observed coccidia of the genera Isospora and Eimeria. The species Isospora parnaitatiaiensis Silva, Rodrigues, Lopes, Berto, Luz, Ferreira, Lopes, 2015 was identified in two different hosts, Pyriglena leucoptera (Vieillot, 1818) and Dysithamnus mentalis (Temminck, 1823), and their oocysts were characterized as polymorphic, since the oocysts from P. leucoptera were more ellipsoidal and the oocysts from D. mentalis were more sub-spherical, which may be the result of speciation process/adaptation to these hosts. The intensities of infection in different hosts were relatively low, since that P. leucoptera and D. mentalis shed together an OoPD of 316 oocysts of I. parnaitatiaiensis, which can be explained by the conserved environment in the Itatiaia National Park and the insectivore feeding habit. Finally, the specificity occurred at the family level, because P. leucoptera and D. mentalis, both of Thamnophilidae family, have been reported as hosts of I. parnaitatiaiensis Os taminofil?deos, da mesma forma que outras fam?lias de Passeriformes, podem ser parasitados por diversas esp?cies de cocc?dios, principalmente dos g?neros Isospora Schneider, 1881 e Eimeria Schneider, 1875. Neste contexto, este trabalho teve por objetivo identificar, caracterizar e quantificar esp?cies de cocc?dios parasitos de Thamnophilidae do PNI. Foram realizadas sete expedi??es no Parque Nacional do Itatiaia, das quais cinco foram em ?reas mais preservadas e duas em ?reas no entorno do parque, as aves foram capturadas com o auxilio de redes de neblina, ao todos foram capturados 184 esp?cimes de aves sendo 26 taminofil?deos, ap?s o processamento das amostras observou ser cocc?dios do g?nero Isospora e Eimeria. A esp?cie Isospora parnaitatiaiensis Silva, Rodrigues, Lopes, Berto, Luz, Ferreira, Lopes, 2015 foi identificada em dois diferentes hospedeiros da fam?lia Thamnophilidae, Pyriglena leucoptera (Vieillot, 1818) e Dysithamnus mentalis (Temminck, 1823), sendo que seus oocistos foram caracterizados como polim?rficos, j? que os oocistos de P. leucoptera s?o mais elips?ides em rela??o aos oocistos de D. mentalis que tendem ao ser mais sub-esf?ricos, o que pode ser consequ?ncia do processo de especia??o/adapta??o ao hospedeiro. A intensidade de infec??o nos diferentes hospedeiros taminofil?deos positivos foram relativamente baixas, P. leucoptera e D. mentalis tiveram juntos um OoPD de 316 para os oocistos de I. parnaitatiaiensis, o que pode ser justificado pelo ambiente conservado do PNI e pelo h?bito alimentar inset?voro. Finalmente, a especificidade ocorreu em n?vel de fam?lia, pelo fato de P. leucoptera e D. mentalis, ambos da fam?lia Thamnophilidae, ter sido relatados como hospedeiros de I. parnaitatiaiensis

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2020-02-07T15:14:08Z No. of bitstreams: 1 2014 - Gide?o da Silva Galv?o.pdf: 13814819 bytes, checksum: faa3b01e9bd8382de994358015c1c535 (MD5) Made available in DSpace on 2020-02-07T15:14:08Z (GMT). No. of bitstreams: 1 2014 - Gide?o da Silva Galv?o.pdf: 13814819 bytes, checksum: faa3b01e9bd8382de994358015c1c535 (MD5) Previous issue date: 2014-02-27 CAPES This study aimed to determine the presence of antibodies against Neospora caninum seropositivity and the distribution among the different age groups in buffalo herds located in the regions that comprise the state of Rio de Janeiro, Brazil. Serum samples from 270 buffaloes categorized into three ages: ? 2 years, 2 to ? 4 years, >5 years were tested by use of an immunofluorescent antibody test with a cutoff of 1:200. The Fisher exact test was used with a significance level of 5 %. The frequency of seropositive animals was 29.76% (86/289). Antibodies against N. caninum was detected in 14 (87.5 %) of 16 properties, with positive ranging from 3.57 to 100%. In the present study we observed differences in seropositivity according to different ages (p = 0.243), and animals aged more than five years had 2.62 greater chance of exposure. The results show the exposure of buffaloes to the agent in the regions studied, one should include the parasite in the differential diagnosis of possible cases of buffalo abortion, and highlights the need to adopt health measures for the control of infection in herds reviews. The final logistic regression model showed the influence of the age variable and the presence of cats across the seropositivity of water buffaloes which suggests that horizontal transmission is the main route of transmission in the properties analyzed. Este estudo teve como objetivo verificar a presen?a de anticorpos anti-Neospora caninum e a distribui??o entre a soropositividade nas diferentes faixas et?rias em rebanhos bubalinos localizados nas mesorregi?es que comp?em o estado do Rio de Janeiro, Brasil. A freq??ncia de animais soropositivos foi de 29,76% (86/289). Anticorpos contra-N. caninum foram detectados em 14 (87,5%) das 16 propriedades, com positividade variando de 3,57 a 100%. Foram analisados 289 animais, destes, 270 bubalinos puderam ser categorizados em tr?s estratos: ? 2 anos , 2 a ?4 anos, >5 anos e foram submetidas ? t?cnica de rea??o de imunofluoresc?ncia indireta com ponto de corte de 1:200, apresentando assim 27,41% de animais sororreagentes (74/270). O teste exato de Fisher foi utilizado com n?vel de signific?ncia de 5%. No presente estudo observaram-se diferen?as em rela??o ? soropositividade em fun??o das diferentes idades (p=0, 0243), e animais com idade superior a cinco anos apresentaram 2,62 mais chances de exposi??o ao agente. Os resultados obtidos evidenciam a exposi??o de bubalinos ao agente nas mesorregi?es estudadas, devendo-se incluir o parasito no diagn?stico diferencial nos poss?veis casos de abortamento bubalino, bem como evidencia a necessidade de ado??o de medidas sanit?rias para o controle da infec??o nos rebanhos avaliados. O modelo final de regress?o log?stica demonstrou a influ?ncia da vari?vel faixa et?ria e da presen?a de gatos frente ? soropositividade dos bubalinos o que sugere que a transmiss?o horizontal seja a principal rota de transmiss?o nas propriedades analisadas.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-11-05T18:24:22Z No. of bitstreams: 1 2014 - Ulisses Jorge Pereira Stelmann.pdf: 2898011 bytes, checksum: 3008e74e285fcb83a0777dc7615797d0 (MD5) Made available in DSpace on 2019-11-05T18:24:22Z (GMT). No. of bitstreams: 1 2014 - Ulisses Jorge Pereira Stelmann.pdf: 2898011 bytes, checksum: 3008e74e285fcb83a0777dc7615797d0 (MD5) Previous issue date: 2014-02-21 CAPES Among the neurological diseases that affect horses, the protozoal myeloencephalitis (EPM) takes an outstanding position, which is characterized by infection of the central nervous system (CNS). The disease was initially attributed to Toxoplasma gondii, however, nowadays it is known that Sarcocystis neurona is the major causing agent of this disease. In spite of this, studies have shown the occurrence of clinical signs similar to those observed in the EPM by S. neurona in the neosporosis associated with the neurological form in horses, which is caused by the species Neospora caninum and Neospora hughesi. The aim of this study was to determine the seroprevalence of S. neurona, Neospora spp. and T. gondii in horses from Serrana microregion of the state of Rio de Janeiro, Brazil. This region is formed by the cities of Petr?polis, Sao Jos? do Vale do Rio Preto and Teres?polis. In addition, were verified the probable factors associated with the presence of these properties in the etiologic agents that participated in this cross sectional study. By convenience, were selected agricultural establishments with equine breeding, regardless of age, sex and race. The choice of animals was non random. Participated in the study 23 agricultural establishments with horse breeding, of which 19 belonged to Petr?polis, three from Teres?polis, and one from municipality of S?o Jos? do Vale do Rio Preto. Blood samples were collected from 375 horses, which were analyzed by indirect immunofluorescence assay (IFA) for antibodies against coccidia of interest in the study. There was a small number of seropositive subjects at three equine protozoal studied, 8.27% (31/375) of horses positive for S. neurona, 1.87% (7/375) on the Neospora spp. and 2.13% (8/375) on T. gondii. In conclusion, S. neurona infection was the most prevalent and presented a greater number of statistically significant results among the variables studied. Infection with Neospora spp. was related to properties with a history of abortion and those where the cats had access to horse?s food. In our study, none of the evaluated variables was statistically significant for T. gondii infection. Even in the presence of favorable conditions for the occurrence of definitive hosts of S. neurona, Neospora spp. and T. gondii, the sanitary conditions of animals and agricultural establishments analyzed contributed to a low occurrence of seropositive horses for the three etiological agents surveyed in the Serrana microregion of the state of Rio de Janeiro, Brazil. Dentre as enfermidades neurol?gicas que acometem equinos, a mieloencefalite por protozo?rio (EPM) assume posi??o de destaque, sendo caracterizada pela infec??o do sistema nervoso central (SNC). Inicialmente atribu?da a Toxoplasma gondii, por?m, sabe se hoje que Sarcocystis neurona ? o principal agente etiol?gico da enfermidade. Todavia, trabalhos demonstraram a ocorr?ncia de sinais cl?nicos, semelhantes ?queles observados na EPM por S. neurona, associados ? forma neurol?gica da neosporose em equinos, que ? causada pelas esp?cies, Neospora caninum e Neospora hughesi. O objetivo deste estudo foi determinar a soropreval?ncia para S. neurona, Neospora spp. e T. gondii, em equinos da microrregi?o Serrana do estado do Rio de Janeiro, constitu?da pelos munic?pios de Petr?polis, S?o Jos? do Vale do Rio Preto e Teres?polis, al?m de verificar os prov?veis fatores associados ? presen?a desses agentes etiol?gicos nas propriedades que participaram deste estudo transversal. Foram selecionados, por conveni?ncia, estabelecimentos agropecu?rios com cria??o de equinos, independentemente da idade, sexo e ra?a. A escolha dos animais foi realizada de forma n?o aleat?ria. Participaram desse estudo 23 estabelecimentos agropecu?rios com cria??o de equinos, dos quais 19 pertenciam a Petr?polis, tr?s a Teres?polis e um ao munic?pio de S?o Jos? do Vale do Rio Preto. Foram coletadas amostras de sangue de 375 equinos, as quais foram analisadas pela rea??o de imunofluoresc?ncia indireta (RIFI) para detec??o de anticorpos contra os cocc?dios de interesse do estudo. Observou se um baixo n?mero de equinos sororreagentes aos tr?s protozo?rios estudados, sendo 8,27% (31/375) dos equinos positivos para S. neurona, 1, 87% (7/375) para o Neospora spp. e 2,13% (8/375) para T. gondii. A infec??o por S. neurona foi a mais prevalente e a que apresentou um n?mero maior de resultados com signific?ncia estat?stica dentre as vari?veis pesquisadas. A infec??o por Neospora spp. esteve relacionada ? propriedades com hist?rico de abortamento e acesso de gatos aos alimentos dos equinos. Nas condi??es deste estudo, nenhuma das vari?veis avaliadas mostrou se estatisticamente significativa para a infec??o por T. gondii. Mesmo em presen?a de condi??es favor?veis para a ocorr?ncia dos hospedeiros definitivos de S. neurona, Neospora spp. e T. gondii, as condi??es higi?nico sanit?rias dos animais e dos estabelecimentos agropecu?rios analisados contribu?ram para uma baixa ocorr?ncia de equinos sororreagentes aos tr?s agentes etiol?gicos pesquisados na microrregi?o Serrana do estado do Rio de Janeiro.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-07-06T14:18:42Z No. of bitstreams: 1 2013 - Thais Ferreira Fagundes.pdf: 1535297 bytes, checksum: c84429e252f88d8630e925d1fb287899 (MD5) Made available in DSpace on 2020-07-06T14:18:42Z (GMT). No. of bitstreams: 1 2013 - Thais Ferreira Fagundes.pdf: 1535297 bytes, checksum: c84429e252f88d8630e925d1fb287899 (MD5) Previous issue date: 2013-03-08 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES Funda??o de Amparo ? Pesquisa do Estado do Rio de Janeiro - FAPERJ This study aimed to perform a descriptive analysis of diarrhea and parasites infection ; the association among diarrhea and risk factors such as infection by Cryptosporidium, Giardia, Eimeria and helminths, age, failure of passive immunity transference (FPT), type of fertilization (in vitro embryo production - IVP, artificial insemination - AI and natural mount), dam (heifer or cow) and birth weight. Moreover, assessing the influence of diarrhea on the weekly weight gain of dairy heifer calves reared in individual calf pens under paddock in a farm of dairy production, Pira?, Rio de Janeiro. From September 2009 to June 2011, feces collection, weighing and data about the calves, were obtained weekly. Blood samples were collected only once after ingestion of colostrum. The calves in this study were followed up from birth to one hundred day old until the end of the study period / or death / or release. Feces were considered normal (firm, brown, tail and perineum clean and dry) or diarrhea (pasty or aqueous, tail and/or perineum soiled with feces). Logistic regression models by generalizes estimated equations were used to study relationship among diarrhea and its determinants factors, the relative risks (RR) were estimated. Gaussian regression models by generalizes estimated equations were used to obtained the relationship between weekly weigh gain and diarrhea. For both models, intervals with 90% confidence were estimated. One hundred and eighteen calves were followed up, in a total of 1457 fecal samples. The incidence rates of diarrhea, infection by Cryptosporidium, G. intestinalis, Eimeria and helminths were 25.7, 13.2, 16.6, 26.9 and 7.3 new cases per 100 calves week, respectively. In calves up to 30 dayold, the type of fertilization IVP [RR=0.33 (CI 90% 0.14 - 0.75)] is a factor protection and Cryptosporidium spp. infection [RR=1.95 (CI 90% 1.22 - 3.12)] risk factor.On the other hand, for calves with 31 to 100 days of age, IVP [RR=0.50 (CI 90% 0.28 -0.89)] and birth weight ? 40 kg [RR= 0.65 (CI 90% 0.47 - 0.89)] are protective factors. , Greater was the weight gain, in those calves more old (p

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-05-06T12:24:38Z No. of bitstreams: 1 2013 - Gilberto Flausino.pdf: 19088218 bytes, checksum: db58c42aa5c4f7760fa43119bf48e49e (MD5) Made available in DSpace on 2020-05-06T12:24:38Z (GMT). No. of bitstreams: 1 2013 - Gilberto Flausino.pdf: 19088218 bytes, checksum: db58c42aa5c4f7760fa43119bf48e49e (MD5) Previous issue date: 2013-02-28 Four creations of guinea pigs from rustic breeders located at the Municipality of Serop?dica in the State of Rio de Janeiro, Brazil, stool samples from 15 animals were collected randomly, where six (46.6%) of them were positive for Cyniclomyces guttulatus. From the same creations, only one was found positive for unsporulated oocysts of Eimeria caviae. Six animals positive for C. guttulatus were posted to isolate vegetative forms of this acomycete. Likewise a single animal positive for C. guttulatus and infected orally as 106 sporulated oocysts of E. caviae was also euthanized with CO2 in chamber to serve as a donor of both forms of endogenous phases of C. guttulatus as well as E. caviae found in scrapings of the cecum and colon mucosa. Smears were stained with Giemsa and Ziehl-Nielsen. The project was submitted from ethics committee of UFRRJ the number getting was 009759. Cyniclomyces guttulatus can be found in the digestive tract and feces of C. porcells as a participant in their microbiota without causing notable injuries. Isolates of C. guttulatus of guinea pigs have phenotypic and genotypic characteristics identical to strains of this species found in rabbits. The strain of E. caviae also isolated from guinea pigs of rustic breedings had similar morphotypes, in spited of has three types of oocysts, small, measuring (18.07 x 16.39 ?m), medium, measuring (20.87 x 17.87 ?m) and large size, measuring (22.80 x 17.92 ?m). Polymorphism among sporulated oocysts can be observed on the histogram class and its regression line. Although the values of DM are significant, the morphology of the sporulated oocysts was not different. The pre-patent and patent were five and seven days, respectively, characterizing the species as the short period. In experimental infection at a dose of 106 sporulated oocysts of E. Caviae, eimeriosis was not observed, because there was no development of clinical signs in animals. The shapes of endogenous C. guttulatus and E. caviae, characterized as vegetative cells and merozoites respectively, were to be examined carefully to avoid any confusion in the differential diagnosis between the two etiological agents in the same vertebrate host. The genotypic characteristics of both, C. Guttulatus and E. caviae from Cavia procellus in Brazil were determined and deposited in GenBank. De quatro cria??es de porquinhos-da-?ndia, localizadas no munic?pio de Serop?dica, Estado do Rio de Janeiro, Brasil, foram coletadas amostras de fezes de 15 animais de maneira aleat?ria onde seis (46,6%) deles foram positivos para Cyniclomyces guttulatus. Dessas col?nias, somente em uma estavam presentes oocistos n?o esporulados de Eimeria caviae nas fezes de um animal. Os seis animais positivos para C. guttulatus foram eutanasiados para proceder ao isolamento de formas vegetativas deste ascomiceto. Da mesma forma, um ?nico animal positivo para C. guttulatus e infectado por via oral com 106 oocistos esporulados foi tamb?m eutanasiado em c?mara de CO2 para servir de doador das formas end?genas tanto de C. guttulatus como de E. caviae encontradas nos raspados de mucosa do ceco e intestino grosso corados pelas t?cnicas de Giemsa e Ziehl-Nielsen. O projeto foi submetido ? comiss?o de ?tica da UFRRJ sob o n?mero 009759. Cyniclomyces guttulatus pode ser encontrado no trato digest?rio e nas fezes de C. porcellus como participante de sua microbiota sem causar les?es dignas de nota. Os isolados de C. guttulatus de cobaios possuem caracter?sticas fenot?pica e genot?pica id?nticas aos isolados desta esp?cie encontrados em coelhos. A cepa de E. caviae isolada de cobaios de cria??es r?sticas tem como morfotipos tr?s tipos de oocistos esporulados, pequenos, medindo em m?dia (18,07 x 16,39 ?m), m?dios, medindo em m?dia (20,87 x 17,87?m) e grandes, medindo em m?dia (22,80 x 17,92?m), onde o polimorfismo pode ser observado atrav?s dos intervalos de classes nos histogramas e de sua regress?o linear. Apesar dos valores, de di?metro maior, serem significativos ? morfologia dos oocistos esporulados n?o foi diferente. Os per?odos pr?-patente e patente foram de cinco e sete dias, respectivamente, caracterizando a esp?cie como de per?odo curto. Em infec??o experimental, na dose de 106 oocistos esporulados de E. caviae n?o causou eimeriose, pois n?o houve desenvolvimento de sinais cl?nicos nos animais. As formas end?genas de C. guttulatus e de E. caviae, caracterizadas como c?lulas vegetativas e merozo?tos respectivamente, foram as que devem ser analisadas criteriosamente para que n?o haja equ?voco no diagn?stico diferencial entre os dois agentes etiol?gicos em um mesmo hospedeiro vertebrado. As caracter?sticas genot?picas, tanto de C. guttulatus, quando de E. caviae encontradas em Cavia procellus no Brasil foram determinadas e depositadas no GenBank

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-10-20T22:39:34Z No. of bitstreams: 1 2011 - Lianna Maria de Carvalho Balthazar.pdf: 7490971 bytes, checksum: c85cf92a1e158d4a77ed4dcf409c92c5 (MD5) Made available in DSpace on 2020-10-20T22:39:34Z (GMT). No. of bitstreams: 1 2011 - Lianna Maria de Carvalho Balthazar.pdf: 7490971 bytes, checksum: c85cf92a1e158d4a77ed4dcf409c92c5 (MD5) Previous issue date: 2011-02-23 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. Coccidiosis as being limiting in nature, often asymptomatic, is observed in most of these cage birds, causing health problems and economic losses for both small and for large breeders.The principal objective of this research was to describe coccidiosis in cage birds located in the City of Teres?polis, from the mountainous region of the State of the Rio de Janeiro. The cage birds are kept as pets for their beauty as for their singing and mostly belong to the order Passeriformes In this study, oocysts recovered from feces of cage birds were species of the genus Isospora Schneider, 1881 identified by the phenotypic and morphometric characteristics. It could be observed that coccidiosis in cage birds in this study did not represent a limiting factor in this system of breeding in spite of the presence of coccidian oocysts in the feces of studied birds. A coccidiose por ser de car?ter limitante, muitas vezes assintom?tica, ? observada na maioria das aves de gaiola, causando problemas de sa?de e preju?zos econ?micos tanto para pequenos quanto para grandes criadores. Este trabalho teve como objetivo principal verificar a infec??o de cocc?dios em p?ssaros de gaiola do munic?pio de Teres?polis, localizado na Regi?o Serrana . As aves de gaiola s?o mantidas como mascotes tanto pela sua beleza como pelo seu canto, e em sua maioria pertencem ? ordem Passeriformes. Neste trabalho, os oocistos observados nas fezes desses p?ssaros de gaiola, foram identificados atrav?s das caracter?sticas fenot?picas e an?lise morfom?trica como esp?cies do g?nero Isospora Schneider, 1881. Observou-se que a coccidiose em p?ssaros de gaiola no presente estudo n?o foi fator limitante no sistema de cria??o, apesar da presen?a de oocistos de cocc?dios nas fezes das aves estudadas.