1. Inhibition of Norovirus GII.4 binding to HBGAs by Sargassum fusiforme polysaccharide.
- Author
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Sun Y, Liang M, Wu M, and Su L
- Subjects
- Animals, Humans, Gastroenteritis virology, Gastroenteritis drug therapy, Antiviral Agents pharmacology, Antiviral Agents chemistry, Ostreidae virology, Virion metabolism, Virion ultrastructure, Virion drug effects, Edible Seaweeds, Norovirus drug effects, Sargassum chemistry, Polysaccharides pharmacology, Polysaccharides chemistry, Polysaccharides metabolism, Blood Group Antigens metabolism, Caliciviridae Infections virology, Caliciviridae Infections drug therapy
- Abstract
Norovirus (NoV) is the main pathogen that causes acute gastroenteritis and brings a heavy socio-economic burden worldwide. In this study, five polysaccharide fractions, labeled pSFP-1-5, were isolated and purified from Sargassum fusiforme (S. fusiforme). In vitro experiments demonstrated that pSFP-5 significantly prevented the binding of type A, B and H histo-blood group antigens (HBGAs) to NoV GII.4 virus-like particles (NoV GII.4 VLPs). In addition, in vivo experiments revealed that pSFP-5 was effective in reducing the accumulation of NoV in oysters, indicating that pSFP-5 could reduce the risk of NoV infection from oyster consumption. The results of transmission electron microscopy showed that the appearance of NoV GII.4 VLPs changed after pSFP-5 treatment, indicating that pSFP-5 may achieve antiviral ability by altering the morphological structure of the viral particles so that they could not bind to HBGAs. The results of the present study indicate that pSFP-5 may be an effective anti-NoV substance and can be used as a potential anti-NoV drug component., (© 2024 The Author(s).)
- Published
- 2024
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