37 results on '"Nikitin SV"'
Search Results
2. Highly energetic N -cyano-substituted CL-20 analogues: challenging the stability limits of polynitro hexaazaisowurtzitanes.
- Author
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Parakhin VV, Pokhvisneva GV, Shlykova NI, Samigullina AI, Nikitin SV, Smirnov GA, Gordeev PB, Kon'kova TS, Lempert DB, and Pivkina AN
- Abstract
To design high-energy-density materials of a new level, it is necessary to develop methods for the functionalization of energetic scaffolds, which will make it possible to tune their physicochemical and energetic properties. For this reason, we have elaborated an approach for synthesizing a new series of energetic cage compounds with advanced properties by introducing the N -cyano group into the polynitro hexaazaisowurtzitane framework. The structures of the obtained substances were fully characterized with a combination of methods, including multinuclear (
1 H,13 C{1 H},14 N, and15 N{1 H}) NMR and IR spectroscopy, high-resolution mass spectrometry, X-ray diffraction analysis, electron microscopy and quantum chemical calculations. For the resulting compounds, thermal stability and safety tests were carried out, calorimetric and pycnometric measurements were performed, and the energetic potential was determined by high-temperature chemical equilibrium thermodynamic calculations. The new cyano derivatives have an acceptable density (up to 1.92 g cm-3 ) and a high enthalpy of formation (up to 2 MJ kg-1 ), which is 2 times that of the benchmark CL-20. The resistance of the target compounds to friction (up to 220 N) is the highest compared to CL-20 and its known analogues. 4,10-Dicyano-2,6,8,12-tetranitro-2,4,6,8,10,12-hexaazaisowurtzitane of the new series is the most thermally stable (a Tdec of 238 °C) among the known energetic polynitro hexaazaisowurtzitanes and is the first derivative of this family to surpass CL-20 in heat resistance. Moreover, the specific impulse for the novel materials showed an improvement of 6.5-13 s over CL-20.- Published
- 2024
- Full Text
- View/download PDF
3. [Correction of serum prooncogenic cytokines and metastases by 5-hydroxypyrimidine derivatives and doxorubicin after removal of a primary tumor node in mice with the Lewis lung epidermoid carcinoma].
- Author
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Kovalenko LP, Korzhova KV, Nikitin SV, Ivanova EA, and Zhurikov RV
- Subjects
- Animals, Mice, Cytokines, Interleukin-4, Interleukin-6, Doxorubicin, Lung, Carcinoma, Lewis Lung, Carcinoma, Non-Small-Cell Lung, Carcinoma, Squamous Cell, Lung Neoplasms
- Abstract
The effect of a single injection of doxorubicin, 8-day administration of two 5-hydroxypyrimidine derivatives, SNK-411 (2-Isobutyl-4,6-dimethyl-5-hydroxypyrimidine) and SNK-578 (hydrochloride of 2-isobutyl-4,6-dimethyl-5-hydroxypyrimidine), on metastases, lifespan and serum cytokines has been investigated in С57ВL/6 mice after removal of a primary tumor node of Lewis lung carcinoma (LLC). LLC cells (1×106) were injected in the footpad of right hind feet of mice in control and experimental groups; after 14 days of tumor development the hind feet with the tumor were amputated at the ankle level. One hour before the amputation mice received a single injection of doxorubicin (4 mg/kg) and 8-day therapy with the 5-hydroxypyrimidine derivatives started. SNK-578 monotherapy was performed at a dose of 10 mg/kg administered intraperitoneally (i.p.). SNK-411 was administered per os at a dose of 25 mg/kg. In the case of combined therapy mice also received a single injection of doxorubicin (4 mg/kg; i.p.). The metastasis inhibition index in mice-treated with SNK-411 and SNK-578 were 53.3% as compared with control mice (with removed tumor). The mice-treated with SNK-411, doxorubicin, and the combination SNK-578 + doxorubicin had lifespan increased by 60.2%, 53.9%, and 42.9%, respectively. A single injection of doxorubicin, the course administration of the 5-hydroxypyrimidine derivative alone and in combination with single injection of doxorubicin completely decreased serum levels of the prooncogenic Th2-cytokines IL-4, and IL-6 and significantly decreased the level of the Th2-cytokine IL-5. Administration of doxorubicin, SNK-411 and SNK-578 did not influence serum concentration of Th1-cytokine interferon gamma (IFN-γ). These data confirm our previous findings that administration of the compounds studied decreased concentrations of prooncogenic IL-4 and IL-6 in tumor-bearing mice with LLC and had no effect on concentrations of the Th1-cytokine IFN-γ.
- Published
- 2023
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4. Unusual congenital polydactyly in mini-pigs from the breeding group of the Institute of Cytology and Genetics (Novosibirsk, Russia).
- Author
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Nikitin SV, Knyazev SP, Trifonov VA, Proskuryakova АА, Shmidt YD, Shatokhin KS, Zaporozhets VI, Bashur DS, Korshunova EV, and Ermolaev VI
- Abstract
The article describes a new phenomenon in the breeding group of mini-pigs at the Institute of Cytology and Genetics (ICG, Novosibirsk): polydactyly (extra digits), which is unusual because the additional digits are situated at the lateral surface of legs or at the lateral and medial ones. This anomaly was f irst found here in 2017 in adult animals intended for culling due to incorrect positioning of the legs caused by f lexor tendon laxity and resulting in weight-bearing on the palmar surface of the proximal phalanges ("bear's paw"). Therefore, the polydactyly of mini-pigs has a pronounced negative selection effect. A visual survey of the livestock was conducted, and a description of the detected anomaly was compiled. The polydactyly in mini-pigs is a stand-alone trait and is not part of any syndromes. Individuals with polydactyly may have extra digits either on pectoral or on pectoral and pelvic limbs. On thoracic limbs, there may be either one lateral digit or a lateral digit and a medially located rudimentary hoof let. On pelvic limbs, only lateral extra digits can occur. Anatomical and morphological analyses showed that the lateral extra digit is an anatomically complete ("mature") structure, whereas the medial rudimentary digit consists of only a hoof let without other structures characteristic of normal digits. Cytological examination revealed no specif ic karyotypic features, except for Robertsonian translocation Rb 16;17 previously reported for the mini-pigs of the same livestock. Cytological f indings indicated that the polydactyly and Robertsonian translocation are not linked genetically. Genealogical analysis and results of crosses are consistent with a working hypothesis of recessive inheritance of the trait. Overall, the study shows that this type of polydactyly is anatomically and morphologically unique and not typical of Sus scrofa. In this species, only polydactyly types with medial accessory toes have been described and are usually inherited as a dominant trait with incomplete penetrance. In our case, the results of test crosses indicate recessive inheritance of the trait with varying expression and incomplete penetrance, because of which poorly expressed phenotypes are not visually detectable., (Copyright © AUTHORS.)
- Published
- 2021
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5. [Effect of 5-hydroxypyrimidine derivatives on tumor growth and cytokine concentration in blood serum of female CBA mice with cervical cancer (RSHM-5)].
- Author
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Kovalenko LP, Korzhova KV, Zainullina LF, Nikitin SV, Ivanova EA, and Zhurikov RV
- Subjects
- Animals, Female, Humans, Injections, Intraperitoneal, Mice, Mice, Inbred CBA, Serum, Cytokines, Uterine Cervical Neoplasms
- Abstract
The effects of intraperitoneal administration of SNK-411 (2-isobutyl-4,6-dimethyl-5-hydroxypyrimidine) in a dose of 25 mg/kg (the total dose of 350 mg/kg) and SNK-578 (hydrochloride of 2-isobutyl-4,6-dimethyl-5- hydroxypyrimidine) in a dose of 10 mg/kg (the total dose of 140 mg/kg) on tumor growth and concentration of cytokines in the blood serum were studied in female CBA mice. Substances were administrated from the 2nd to 15th days of tumor development. Tumor growth inhibition (TGI) and serum cytokine level were studied on the 7th day after the end of compounds administration (21st day of tumor growth). In intact control group (n=10) median tumor mass was 1255 mg. TGI in the group of animals treated with SNK-411 was 47%; in the group of mice treated with SNK-578 TGI was 87%, tumor mass demonstrated 7.4-fold reduction. Serum concentrations of cytokines (IL-6, IL-10, IL-17A and IFN-γ) in tumor-bearing group of mice were higher versus the intact control group by 229%, 40%, 60% and 81%, respectively. Highly active SNK-578 decreased concentrations of prooncogenic IL-10, IL-17A and proinflammatory IL-6, by 61%, 70% and 29% as compared to tumor-bearing control group. SNK-411 decreased concentrations of prooncogenic IL-10 and IL-17A by 48% and 60%, respectively, and did not affect concentration of IL-6. Taking into consideration that IL-6 participates in autoimmune reactions, we can assume that the immune control is one of the crucial mechanisms of antitumor effect of SNK-578. All results are statistically significant.
- Published
- 2021
- Full Text
- View/download PDF
6. Effects of 5-Hydroxypyrimidine Derivatives on Tumor Growth and Lifespan of C57BL/6 Mice with Epidermoid Lung Carcinoma.
- Author
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Korzhova KV, Kovalenko LP, Ivanova EA, Nikitin SV, and Durnev AD
- Subjects
- Animals, Doxorubicin therapeutic use, Longevity drug effects, Male, Mice, Mice, Inbred C57BL, Pyrimidines therapeutic use, Tumor Burden drug effects, Antineoplastic Agents chemistry, Antineoplastic Agents therapeutic use, Carcinoma, Lewis Lung drug therapy, Lung Neoplasms drug therapy, Pyrimidines chemistry
- Abstract
The effects of 5-hydroxypyrimidine derivatives SNK-411 (2-isobutyl-4,6-dimethyl-5-hydroxypyrimidine) and SNK-578 (2-isobutyl-4,6-dimethyl-5-hydroxypyrimidine chlorohydrate) on the tumor growth and survival of male C57BL/6 mice with transplanted Lewis lung epidermoid carcinoma (LLC) were studied in animals receiving intraperitoneal treatment on days 2-15 of tumor development. Compound SNK-578 in a dose of 10 mg/kg significantly inhibited tumor growth (by 3.6 times; 72.2%) in 7 days after the treatment was discontinued, while compound SNK-411 in a dose of 25 mg/kg only negligibly reduced tumor volume (by 41.7%). A combination of course of SNK-411 (25 mg/kg) and single intraperitoneal dose of doxorubicin (4 mg/kg) significantly inhibited the tumor growth (by 2.2 times; 55.2%), while the combination of SNK-578 (10 mg/kg) with doxorubicin (4 mg/kg) was in fact ineffective. The median survival of animals with untreated LLC was 28 days. Median survival of mice injected with SNK-578 (10 mg/kg) was 43 days, hence, the lifespan of mice with LLC was by 38.6% longer after the treatment. Two of ten mice in this group developed no tumors.
- Published
- 2020
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7. Effect of 2-Isobutyl-4,6-dimethyl-5-hydroxypyrimidine on the Growth of Lewis Lung Carcinoma and Survival of Mice.
- Author
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Kovakenko LP, Kuznetsova OS, Tallerova AV, Nikitin SV, and Durnev AD
- Subjects
- Animals, Carcinoma, Lewis Lung pathology, Carcinoma, Squamous Cell pathology, Doxorubicin administration & dosage, Drug Screening Assays, Antitumor, Injections, Intraperitoneal, Male, Mice, Inbred C57BL, Neoplasm Transplantation, Survival Analysis, Tegafur administration & dosage, Tumor Burden drug effects, Antineoplastic Agents administration & dosage, Carcinoma, Lewis Lung drug therapy, Carcinoma, Squamous Cell drug therapy, Pyrimidines administration & dosage
- Abstract
Experiments on male C57Bl/6 mice with transplantable Lewis lung epidermoid carcinoma were performed to evaluate the effect of early or delayed administration of compound SNK-411 (2-isobutyl-4,6-dimethyl-5-hydroxypyrimidine) on tumor growth and animal survival. Intraperitoneal injection of SNK-411 in doses of 25 and 50 mg/kg on days 2-8 of tumor growth was followed by significant inhibition of tumor growth (by 1.8 and 2.2 times, respectively, in comparison with the untreated control). Administration of this compound on days 8-15 of tumor development had little effect on tumor growth. SNK-411 in doses of 25 and 50 mg/kg (both dosing regimens) significantly increased survival rate and lifespan of animals with tumors. The drugs for verification of this model (Fluorofur, 400 mg/kg; and doxorubicin hydrochloride, 4 mg/kg) were injected intraperitoneally on days 2-4 and 8-10 of tumor development. Administration of Fluorofur and doxorubicin hydrochloride at the late stage was accompanied by significant inhibition of tumor growth (by 1.6 and 1.4 times, respectively). The increase in the survival rate of animals receiving the cytostatic according to standard dosing regimens was less significant than in experiments with SNK-411.
- Published
- 2016
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8. Effects of 5-Pyrimidinol Derivative SNK-41 on Cytokine Profile of Mice with Lewis Lung Carcinoma.
- Author
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Kuznetsova OS, Tallerova AV, Nikitin SV, and Kovalenko LP
- Subjects
- Animals, Carcinoma, Lewis Lung drug therapy, Carcinoma, Lewis Lung pathology, Disease Models, Animal, Mice, Mice, Inbred C57BL, Antineoplastic Agents pharmacology, Interleukin-2 blood, Interleukin-4 blood, Interleukin-6 blood, Pyrimidines pharmacology
- Abstract
We studied the effects of SNK-411, a new 5-pyrimidinol derivative, on serum cytokine profile of C57Bl/6 mice with Lewis lung carcinoma. The compound was injected intraperitoneally in doses of 25 and 50 mg/kg. A significant (by 3.5 times) increase in serum IL-4 content was detected in mice with tumors on day 9 of tumor development. In mice receiving SNK-411 in doses of 25 and 50 mg/kg, IL-4 content significantly decreased (by 4.0 and 3.6 times) on days 2-8 of carcinoma development; IL-2 content decreased by 1.4 and 1.2 times and IL-6 content decreased by 2.7 and 1.6 times, respectively, in comparison with control mice with tumors. Injections of SNK-411 in the same doses on days 8-15 of carcinoma development led to a significant decrease in IL-4 levels (by 2.2 and 4.5 times, respectively, in comparison with control mice with tumors) and did not affect serum levels of other cytokines.
- Published
- 2016
- Full Text
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9. Application of 3-D imaging sensor for tracking minipigs in the open field test.
- Author
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Kulikov VA, Khotskin NV, Nikitin SV, Lankin VS, Kulikov AV, and Trapezov OV
- Subjects
- Actigraphy instrumentation, Algorithms, Animals, Female, Imaging, Three-Dimensional instrumentation, Male, Pigmentation, Sex Characteristics, Swine, Swine, Miniature, Actigraphy methods, Exploratory Behavior, Imaging, Three-Dimensional methods, Motor Activity, Neuropsychological Tests, Pattern Recognition, Automated methods
- Abstract
Background: The minipig is a promising model in neurobiology and psychopharmacology. However, automated tracking of minipig behavior is still unresolved problem., New Method: The study was carried out on white, agouti and black (or spotted) minipiglets (n=108) bred in the Institute of Cytology and Genetics. New method of automated tracking of minipig behavior is based on Microsoft Kinect 3-D image sensor and the 3-D image reconstruction with EthoStudio software. The algorithms of distance run and time in the center evaluation were adapted for 3-D image data and new algorithm of vertical activity quantification was developed., Results: The 3-D imaging system successfully detects white, black, spotted and agouti pigs in the open field test (OFT). No effect of sex or color on horizontal (distance run), vertical activities and time in the center was shown. Agouti pigs explored the arena more intensive than white or black animals, respectively. The OFT behavioral traits were compared with the fear reaction to experimenter. Time in the center of the OFT was positively correlated with fear reaction rank (ρ=0.21, p<0.05). Black pigs were significantly more fearful compared with white or agouti animals., Comparison With Existing Method: The 3-D imaging system has three advantages over existing automated tracking systems: it avoids perspective distortion, distinguishes animals any color from any background and automatically evaluates vertical activity., Conclusion: The 3-D imaging system can be successfully applied for automated measurement of minipig behavior in neurobiological and psychopharmacological experiments., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
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10. [Genetic components and the uncertainty of the phenotypic realization of the mass of newborns in domestic pigs Sus scrofa L].
- Author
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Nikitin SV, Kniazev SP, and Ermolaev VI
- Subjects
- Alleles, Animals, Birth Weight, Genotype, Humans, Infant, Newborn, Sus scrofa growth & development, Models, Genetic, Quantitative Trait Loci genetics, Sus scrofa genetics
- Abstract
In this article, we discuss the features of the genetic determination of a continuous quantitative trait, the mass of newborn offspring in populations of the domestic pig. We defined several components that determine the phenotypic trait, such as the maternal effect, complete dominance, interaction of the parental alleles in the genotype of the offspring, and the uncertainty of phenotypic realization of genotype. We found that a phenotypic trait of high genetic determinacy can also have a maximum range in phenotypic realization, in which case each genotype encountered in the population can realize within the entire range of possible phenotypes.
- Published
- 2014
11. [Model of genetic control of the number and location of nipples in domestic pig].
- Author
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Nikitin SV, Kniazev SP, and Ermolaev VI
- Subjects
- Animals, Female, Male, Genetic Variation, Mammary Glands, Animal, Models, Genetic, Nipples, Quantitative Trait Loci, Sus scrofa genetics
- Abstract
In domestic pig, the number and location of nipples is considered a single quantitative trait, which has a complex spatial structure that includes bilaterality and a linear sequence of repetitive elements. According to this hypothesis, six inadherent biallelic loci are sufficient for a variety of traits observed in domestic pig populations. Verification of representative samplings from five domestic pig populations of different origin (about 20 000 individuals) confirmed the theses of the hypothesis, this fact allows considering it as a model of the studied bilateral trait. Using the model, the intraclass pheno- and genotypic diversity was examined and the relationship of the trait manifestation asymmetry with heterozygosity of loci that control it was shown.
- Published
- 2012
12. [Expression of estrogen and progesterone receptors in the endometrium of fertile and infertile women].
- Author
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Kostiuchek IN, Vitkina OA, Nikitin SV, Kuchina PV, Balashova NN, Kleshchev MA, Tolibova GKh, Miliutina IuP, and Kvetnoĭ IM
- Subjects
- Adolescent, Adult, Biopsy, Endometrium pathology, Female, Humans, Infertility, Female pathology, Middle Aged, Endometrium metabolism, Gene Expression Regulation, Infertility, Female metabolism, Menstrual Cycle, Receptors, Estrogen biosynthesis, Receptors, Progesterone biosynthesis
- Abstract
The aim of the research is investigation of the endometrial hormonal status during menstrual cycle infertile and infertile women. 68 endometrial biopsies from 18-45 years old women have been studied. Some changes of ER and PR expression during menstrual cycle both in fertile and infertile women has been founded. However the ER expression in endometrial stroma and PR in the glands in a proliferative stage of cycle of fertile women are higher than in a secretory stage. In infertile women the PR expression in endometrial glands is opposite.
- Published
- 2011
13. [Standardized selection and its consequences for genetic population structure (examplified by Sus scrofa L.)].
- Author
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Kniazev SP and Nikitin SV
- Subjects
- Animals, Breeding, Female, Phenotype, Reproduction, Selection, Genetic, Sus scrofa anatomy & histology, Sus scrofa physiology, Sus scrofa genetics
- Abstract
Type of selection for total fitness, specific for domestic animals and termed by us standardizing selection, is discussed. Consequences of such selection for the population structure genetic are considered taking a population of domestic pigs as an example. Examples of effective and ineffective standardizing selection are presented. Specific features of genetic determination often quantitative traits detected in analysis of standardizing selection are discussed.
- Published
- 2011
14. [Ocular and orbital blood flow in apparently healthy adults].
- Author
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Leliuk VG, Golovin DA, Leliuk SE, Nikitin SV, and Dziova FS
- Subjects
- Adolescent, Adult, Brachiocephalic Trunk diagnostic imaging, Brachiocephalic Trunk physiology, Carotid Arteries diagnostic imaging, Carotid Arteries physiology, Eye ultrastructure, Female, Humans, Middle Aged, Ophthalmic Artery diagnostic imaging, Orbit diagnostic imaging, ROC Curve, Reference Values, Retinal Artery diagnostic imaging, Retinal Vein diagnostic imaging, Ultrasonography, Doppler, Color methods, Young Adult, Blood Flow Velocity physiology, Eye blood supply, Ophthalmic Artery physiology, Orbit blood supply, Regional Blood Flow physiology, Retinal Artery physiology, Retinal Vein physiology
- Abstract
Doppler measurements of ocular and orbital blood flow at rest were studied using duplex ultrasonography in 30 apparently healthy adult volunteers. The following linear and calculated Doppler measurements were estimated at rest: blood flow in ophthalmic artery (OA), central retinal artery (CRA), posterior ciliary arteries, central retinal vein and superior ophthalmic vein (SOV). In 10 patients Doppler measurements of blood flow in OA, CRA SOV were estimated 5 times by 2 observers with different level of skills in ultrasonographic ophthalmologic examination. Statistic analysis showed strong correlation of linear and calculated measurements in OA, CRA and venous blood flow velocity. A number of Doppler measurements with the most significant inter- and intraobserver variation were revealed. Measurements with high reproducibility were determined. Thus, a unique technique should be performed in estimation of Doppler measurements of blood flow in ocular and orbital vessels. The velocities in the main arteries (OA, CRA) and veins (SOV) were showed to have significant strong interobserver correlation. The highest reproducibility and accuracy was revealed for maximal systolic velocity in OA and CRA and maximal velocity for cardiac cycle in SOV.
- Published
- 2011
15. [Dynamics of heterozygosity and its correlation with fitness in a population of domestic pigs].
- Author
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Nikitin SV, Kniazev SP, Shvebel' TI, and Goncharenko GM
- Subjects
- Animals, Species Specificity, Alleles, Blood Group Antigens genetics, Heterozygote, Quantitative Trait Loci genetics, Sus scrofa genetics
- Abstract
Variation of heterozygosity at 11 loci for blood group systems (erythrocyte antigens), occurred during 14 years in the domestic pig population of Kemerovskaya breed is described. The two estimates computed for the population examined were represented by expected population heterozygosity, as a measure resistant to stochastic fluctuations, and individual heterozygosity, as a measure with the features of a quantitative trait. Our results showed that relative fitness of genotypic classes, formed by the alleles of erythrocyte antigen loci, was different. It was demonstrated that the population examined carried the alleles responsible for fitness decrease, as well as the alleles with stable and unstable equilibrium points (with increased and decreased relative fitness of heterozygotes). Suggestions based on these results, could be applied not only to the population examined, but also to the domesticated form of Sus scrofa as a whole.
- Published
- 2009
16. [Frequency of chromosomes carrying endogenous retroviruses in the populations of domestic pig and wild boar].
- Author
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Nikitin SV, Iudin NS, Kniazev SP, Aĭtnazarov RB, Kobzev VF, Bekenev VA, Savvina MA, and Ermolaev VI
- Subjects
- Animals, Genetics, Population methods, Haplotypes, Species Specificity, Chromosomes, Mammalian genetics, Retroviridae genetics, Swine genetics
- Abstract
Statistical analysis of the frequency of chromosomes carrying three types (A, B, and C) of porcine endogenous retroviruses (PERV) was based on molecular-genetic testing of populations of domestic pigs and wild boars. Domestic pigs were shown to have higher frequency of PERV and haplotypes containing two and three types of PERV than wild boars.
- Published
- 2008
17. [Sex-linked erythrocyte antigens of the domestic pig].
- Author
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Nikitin SV, Khiazev SP, Goncharenko GM, and Bekenev VA
- Subjects
- Animals, Blood Group Antigens immunology, Haplotypes immunology, Humans, Sex Chromosomes immunology, Sus scrofa immunology, Blood Group Antigens genetics, Erythrocytes immunology, Haplotypes genetics, Quantitative Trait Loci immunology, Sex Chromosomes genetics, Sus scrofa genetics
- Abstract
It has been demonstrated that the domestic pig has a blood group systems whose haplotypes (complex alleles) are formed by at least three closely linked loci that are located, like the MIC2 locus of human blood group systems, in the homologous region of sex chromosomes.
- Published
- 2007
18. [Modeling the structure of supergenes controlling some polyallelic blood group systems in the pig Sus scrofa and horse Equus caballus].
- Author
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Kniazev SP and Nikitin SV
- Subjects
- Alleles, Amino Acid Sequence, Animals, Molecular Sequence Data, Mutation, Phylogeny, Polymorphism, Genetic, Recombination, Genetic, Blood Group Antigens classification, Blood Group Antigens genetics, Horses genetics, Models, Genetic, Sus scrofa genetics
- Abstract
Two polymorphic blood group systems (E and M) of the pig Sus scrofa L. and one blood group system (D) of the horse Equus caballus L. have been studied. On the basis of phenogroup analysis, models describing the formation of the complex allele spectra of these systems and reflecting the contributions of mutations and recombinations have been constructed. The complementary relationships between the antigens determined by the variants of supergenes within the systems, as ell as the probable number and relative positions of the subloci encoding individual groups of antigens in them, have been determined.
- Published
- 2007
19. [Organization of sanitary and epidemiological measures to protect the Omsk region involved in epizootia of avian flu].
- Author
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Erofeev IuV, Uskov PA, Kriga AS, Nikitin SV, Vaĭtovich MA, Burashnikova IP, Anpilova NG, Ukrainets NV, and Turchaninov DV
- Subjects
- Animal Migration, Animals, Antibodies, Viral blood, Diagnosis, Differential, Disease Outbreaks prevention & control, Humans, Influenza A Virus, H5N1 Subtype immunology, Influenza in Birds epidemiology, Influenza, Human blood, Mortality, Risk Factors, Siberia epidemiology, Zoonoses epidemiology, Disease Outbreaks veterinary, Disease Reservoirs veterinary, Influenza in Birds prevention & control, Influenza, Human diagnosis, Poultry
- Abstract
In article organizational aspects of carrying out preventive and antiepidemic actions at a regional level are considered at the bird's flu epizootia. Epizootic researches have shown, that infection of poultry has taken place as a result of contact to a wild bird. Serological inspection 521 persons on presence of antibodies to a influenza A (H5N1) has not revealed seropositive persons. The conclusion, that the forecast of development of a situation for spring of 2006 is made in view of a direction of migration of a wild waterfowl is unfavourable.
- Published
- 2006
20. [Diversity of wild and domestic pig populations estimated by a set of serum allotypes].
- Author
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Nikitin SV, Kniazev SP, Nikolaev AG, Volokh AM, Kirichenko AV, Savina MA, Ermolaev VI, Iudina OP, Bekenev VA, and Aĭtnazarov RB
- Subjects
- Animals, Genetic Markers, Immunoglobulin Allotypes blood, Phenotype, Sus scrofa genetics, Swine genetics, Genetic Variation, Immunoglobulin Allotypes genetics, Sus scrofa classification, Swine classification
- Abstract
Variation of serum protein allotypes serving as genetic markers of the blood has been analyzed in 29 populations of the domestic pig and subspecies of the wild boar. The population biodiversity and genetic structure have been estimated by two methods: by the frequencies of allotype combinations and with the use of a map constructed in the space of two principal components. The results obtained are the basis for determining the characteristics of the microevolution of wild boars and formation of the breeds of domestic pigs.
- Published
- 2006
21. [Hygienic assessment of water supply in a rural area].
- Author
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Nikitin SV and Mubarakshin RR
- Subjects
- Environmental Illness epidemiology, Environmental Illness prevention & control, Follow-Up Studies, Humans, Incidence, Retrospective Studies, Russia epidemiology, Water analysis, Water Pollutants analysis, Hygiene, Rural Population, Water standards, Water Supply standards
- Published
- 2005
22. [The clinical-morphological estimation of the results of laser osteoperforation in treatment of chronic osteomyelitis].
- Author
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Krochek IV, Privalov VA, Lappa AV, and Nikitin SV
- Subjects
- Child, Chronic Disease, Densitometry, Enterobacteriaceae Infections microbiology, Female, Humans, Osteomyelitis microbiology, Remission Induction, Staphylococcal Infections microbiology, Streptococcal Infections microbiology, Time Factors, Treatment Outcome, Laser Therapy methods, Osteomyelitis pathology, Osteomyelitis surgery
- Abstract
The method of laser osteoperforation was approved in 135 patients with chronic osteomyelitis. The diode laser with wave length 970 mm and peak power up to 30 Wt was used. Energy was delivered by the percutaneous contact method with a quartz light guide of 0.4 mm diameter. All the patients had a distinct positive reaction in the nearest days after operation. The fistulas were closed within 4-10 days. Stable remission was noted during 2-5 years by objective indices (X-ray, fluometry, densitometry, morphological data). Our experiences with clinical use of laser osteoperforation show its high efficiency in treatment of chronic osteomyelitis. The method is minimally invasive and allows to considerably shorten the terms of treatment and rehabilitation.
- Published
- 2004
23. [Hygienic evaluation of health of children in an agricultural area].
- Author
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Nikitin SV, Ogleznev GA, and Basov IuN
- Subjects
- Agriculture, Child, Health Status Indicators, Humans, Russia epidemiology, Hygiene, Rural Population
- Published
- 2003
24. [Age variation of the phenotypic expression and the inheritance of the IgG system of immunoglobulin allotypes in the pig].
- Author
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Nikitin SV, Savina MA, Kniazev SP, Gorelov IG, Bekenev VA, Iudina OP, Novikov AA, and Ermolaev VI
- Subjects
- Age Factors, Animals, Crosses, Genetic, Female, Genetic Variation, Models, Genetic, Phenotype, Species Specificity, Immunoglobulin G genetics, Swine genetics, Swine immunology
- Abstract
The specificity of phenotypic expression and inheritance of immunoglobulin allotypes IgG1a and IgG2b in pigs are discussed. It was shown that a negative state by both these allotypes is repeatedly found among newborn pigs but is extremely rare in pigs older than one month. A model, which simultaneously describes the genetic determination of allogroups formed by allotypes IgG1a and IgG2b and the dynamics of ontogenetic expression of individual genotype by system IgG with a result of a visually registered phenotype, was developed. Allele frequencies by system IgG were assessed in populations of domestic pigs of productive breeds, laboratory miniature pigs, and wild Eurasian pigs.
- Published
- 2002
25. [A2G--a new system of alpha-2-globulin allotypes in pig blood serum].
- Author
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Savina MA, Kniazev SP, Ermolaev VI, Nikitin SV, Mitichashvili RS, Khizhniak AA, Gorelov IG, and Bekenev VA
- Subjects
- Animals, Immunodiffusion, Isoantibodies immunology, Isoantigens genetics, Isoantigens immunology, Swine blood, alpha-Macroglobulins immunology, Alleles, Swine genetics, alpha-Macroglobulins genetics
- Abstract
Combinations of four alpha-2-globulin allotypes were studied for their distribution in pigs of nine different breeds and hybrid groups. Based on this analysis, a new, previously unpublished polyallelic genetic system designated A2G was postulated. The complex alleles of this system control alpha-2-globulin allotypes and are suggested to be encoded by genes of two subloci. One of these subloci is virtually monomorphic, whereas the other has at least four allelic variants.
- Published
- 2001
26. [Interaction of polymorphic systems in a population of Semirechenskaya swine breed].
- Author
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Gorelov IG, Nikitin SV, Orlova GV, Khizhniak AA, Savina MA, and Ermolaev VI
- Subjects
- Animals, Genetic Markers, Polymorphism, Genetic, Swine genetics
- Abstract
Associations between the alleles of nonlinked polymorphic genetic systems were studied in the population of the Semirechenskaya breed of pigs. The study of marker pairs of 21 genetic systems revealed that 7% of associations are statistically significant. Most of the associations detected are assumed to arise from breeding by commercial productivity traits and from the formation of an integral genotype adapted to conditions of artificial selection in the population.
- Published
- 2000
27. [The association of genetic markers in two related swine breeds].
- Author
-
Kniazev SP, Nikitin SV, Gorelov IG, Ermolaev VI, Orlova GV, Savina MA, Zhuchaev KV, Papshev SP, and Kabanenko EV
- Subjects
- Animals, Phylogeny, Quantitative Trait, Heritable, Selection, Genetic, Siberia, Species Specificity, Blood Group Antigens genetics, Genetic Linkage, Genetic Markers, Immunoglobulin Allotypes genetics, Polymorphism, Genetic, Swine genetics
- Abstract
The combinations of genetic markers (allotypes, blood-group systems, and polymorphic proteins) were analyzed in pigs of two phylogenetically related Siberian populations: the newly selected Siberian specialized meat breed SM-1 (daughter breed) and the reproductive core of the Kemerovo breed (maternal breed). Statistically significant interlocus associations were found between the genetic markers that are known not to be linked, being assigned to different linkage groups, and the main selected meat-productivity traits. Some of these associations were shared by both breeds; others were found to be specific to either maternal or daughter populations. Possible genetic mechanisms underlying these associations and their significance are discussed.
- Published
- 1999
28. [Associations between the coat color and the blood-group system D antigens in horses].
- Author
-
Kniazev SP and Nikitin SV
- Subjects
- Animals, Breeding, Hair Color immunology, Horses immunology, Selection, Genetic, Genetic Markers, Genetic Variation, Hair Color genetics, Horses genetics, Rh-Hr Blood-Group System genetics
- Abstract
The study of the association between the coat-color variants and the blood-group system D antigens in the populations of two related trotter breeds (Orlov Trotter and Russian Trotter) showed the presence of three associations between these characters in the Orlov Trotter breed. In the populations of Russian trotters, these associations were not detected. Possible reasons for the formation and maintenance of these associations and the role of the selection for coat color in the differentiation of breeds by the frequencies of some system D antigens are discussed.
- Published
- 1999
29. [Linkage of a porcine esterase D locus with a lethal factor].
- Author
-
Orlova GV, Nikitin SV, Bekenev VA, and Lobkov IuI
- Subjects
- Alleles, Animals, Heterozygote, Homozygote, Carboxylesterase, Carboxylic Ester Hydrolases genetics, Genes, Lethal, Genetic Linkage, Swine genetics
- Abstract
This work is devoted to a linkage study of the gene controlling esterase D and a lethal factor in domestic pig. Segregation for two alleles EsDB, which originated from populations of miniature swine and pigs of a large white breed, and the effect of these variants on the number of offspring in a litter were compared. The results showed a linkage of locus EsD with a lethal factor. This lethal factor causes the mortality of homozygotes and half of the heterozygotes in a litter, which depends on the litter size. The lethal factor was found to be absent in miniature pigs, and in the large white breed it is predominantly linked with allele EsDB. The effect of the lethal factor on population structure and fecundity is discussed.
- Published
- 1999
30. [Lethal allele of the Spotted (gray spots) gene in swine].
- Author
-
Nikitin SV and Lobkov IuI
- Subjects
- Alleles, Animals, Heterozygote, Swine, Genes, Lethal, Skin Pigmentation genetics, Swine, Miniature genetics
- Abstract
Disturbances in segregation of offspring with white and colored coats obtained from crossing pigs heterozygous for gene White (a dominant white coat color) with heterozygotes and homozygotes for recessive alleles are studied. The data obtained, along with available data published earlier, suggest the existence of three alleles of gene Spotted, which is linked to gene White. These are a dominant allele Sp (lack of spots), a recessive lethal allele Splet (lack of spots; prenatal death of the homozygotes) and a recessive allele sp (spots in the pigs heterozygous for gene White).
- Published
- 1996
31. [The effect of hypochlorite on electrical surface properties of human blood lipoproteins].
- Author
-
Panasenko OM, Evgina SA, Nikitin SV, and Sergienko VI
- Subjects
- Electricity, Electron Spin Resonance Spectroscopy, Electrophoresis, Polyacrylamide Gel, Humans, Lipoproteins, LDL blood, Lipoproteins, VLDL blood, Manganese chemistry, Spin Labels, Lipoproteins, LDL chemistry, Lipoproteins, VLDL chemistry, Sodium Hypochlorite pharmacology
- Abstract
Surface potential of human blood lipoproteins was studied by the use of Mn(2+)-ions as a positively charged spin probe. The calculated values of surface potential of low density lipoproteins (LDL) appeared to be -17.7 +/- 1.2 mV. It was shown that hypochlorite (OCl-) induced an increase of negative surface potential of LDL. Agarose gel electrophoresis was used for the comparative analysis of electrophoretic mobility of human blood lipoproteins of different classes. It was shown that the interaction of hypochlorite with lipoproteins caused the partial degradation of lipoproteins and the increase in surface charge. High density lipoproteins appeared to be more resistant to the action of OCl- than LDL and especially very low density lipoproteins.
- Published
- 1995
32. [Comparison of neurotropic and stress-protective properties of piracetam and the derivative pyrido]1,2-a]pyrimidine].
- Author
-
Kozlovskaia MM, Inozemtsev AN, Nikitin SV, Gochmuradov AG, Iakushev RA, and Chabak-Gorbach R
- Subjects
- Animals, Male, Rats, Nootropic Agents therapeutic use, Piracetam therapeutic use, Pyrimidines therapeutic use, Stress, Psychological prevention & control
- Published
- 1995
33. [Serum lipoprotein (A) in patients with myocardial infarction and aortoarteritis].
- Author
-
Tvorogova MG, Niiazova LE, Nikitin SV, Sugraliev AB, Kotkina TI, and Titov VN
- Subjects
- Acute-Phase Proteins analysis, Adolescent, Adult, Aged, Aortitis complications, Apolipoproteins A analysis, Arteritis complications, Chronic Disease, Female, Humans, Male, Middle Aged, Myocardial Infarction complications, Aortitis blood, Arteritis blood, Lipoprotein(a) blood, Myocardial Infarction blood
- Abstract
To elucidate the physiological role of lipoprotein (a), a new independent atherosclerosis risk factor, the levels of lipoprotein (a) and 5 proteins of an acute phase were measured in acute (myocardial infarction, n = 21) and chronic (non-specific aortoarteritis, n = 15) tissue lesions. Blood was taken for analysis from patients with myocardial infarction when they were admitted to hospital for anginal attacks and then 4 times during a month and from patients with aortoarteritis at the stage of exacerbation 1 month before and after therapy. The findings indicate that in acute and chronic tissue lesions, there is a change in blood lipoprotein (a) levels, which is largely similar to that in the levels of acute phase proteins. One cause of such changes may be an increase in the synthesis of apolipoprotein (a) that is identical to that of other glycoproteins--acute phase proteins--in the reaction of the acute phase and then in the regeneration of damaged tissues.
- Published
- 1992
34. [Lipoprotein(a)--a risk factor for coronary atherosclerosis].
- Author
-
Titov VN, Tvorogova MG, and Nikitin SV
- Subjects
- Aging blood, Coronary Artery Disease epidemiology, Humans, Hypercholesterolemia blood, Hyperlipoproteinemia Type II blood, Reference Values, Risk Factors, Coronary Artery Disease blood, Lipoprotein(a) blood
- Published
- 1992
35. [Apolipoprotein E as a risk factor of coronary arteriosclerosis].
- Author
-
Titov VN, Nikitin SV, and Tvorogova MG
- Subjects
- Apolipoproteins E genetics, Coronary Artery Disease genetics, Humans, Lipids genetics, Phenotype, Risk Factors, Apolipoproteins E metabolism, Coronary Artery Disease etiology, Lipid Metabolism
- Published
- 1991
36. [Mapping of the locus for the H blood-group system on the 15th chromosome of domestic swine].
- Author
-
Tikhonov VN, Gorelov IG, Nikitin SV, Bobovich VE, and Astakhova NM
- Subjects
- Animals, Crosses, Genetic, Female, Genotype, Heterozygote, Male, Recombination, Genetic, Blood Group Antigens genetics, Chromosome Mapping, Genetic Markers, Swine blood
- Published
- 1983
37. [Central and peripheral hemodynamics in newborn infants in the first few days of life].
- Author
-
Nikitin SV, Efimov MS, Burakova VB, Aksel'rod VG, and Andreĭko TA
- Subjects
- Age Factors, Blood Volume, Humans, Infant, Premature, Hemodynamics, Infant, Newborn
- Published
- 1981
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