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1. STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment

Author

Lohard, Steven, Bourgeois, Nathalie, Maillet, Laurent, Gautier, Fabien, Fétiveau, Aurélie, Lasla, Hamza, Nguyen, Frédérique, Vuillier, Céline, Dumont, Alison, Moreau-Aubry, Agnès, Frapin, Morgane, David, Laurent, Loussouarn, Delphine, Kerdraon, Olivier, Campone, Mario, Jézéquel, Pascal, Juin, Philippe P., and Barillé-Nion, Sophie

Published
2020
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6. Mammary carcinoma: Comparative oncology between small animals and humans—New therapeutic tools.

Author

Frénel, Jean‐Sébastien and Nguyen, Frédérique

Subjects
*ONCOLOGY, *THERAPY dogs, *TUMOR classification, *HORMONE therapy, *COMBINED modality therapy, *DOGS, *CATS
Abstract

The poor outcomes associated with mammary carcinomas (MCs) in dogs and cats in terms of locoregional recurrence, distant metastasis and survival, highlight the need for better management of mammary cancers in small animals. By contrast, the outcomes of women with breast cancer (BC) have dramatically improved during the last 10 years, notably thanks to new therapeutic strategies. The aim of this article was to imagine what could be the future of therapy for dogs and cats with MCs if it became inspired from current practices in human BC. This article focuses on the importance of taking into account cancer stage and cancer subtypes in therapeutic plans, on locoregional treatments (surgery, radiation therapy), new developments in endocrine therapy, chemotherapy, PARP inhibitors and immunotherapy. Ideally, multimodal treatment regimens would be chosen according to cancer stage and cancer subtypes, and according to predictive factors that are still to be defined. [ABSTRACT FROM AUTHOR]

Published
2023
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13. Supplementary_methods_1 – Supplemental material for Identification of an immune-suppressed subtype of feline triple-negative basal-like invasive mammary carcinomas, spontaneous models of breast cancer

Author

Dagher, Elie, Simbault, Laura, Abadie, Jérôme, Loussouarn, Delphine, Campone, Mario, and Nguyen, Frédérique

Subjects
FOS: Clinical medicine, Cell Biology, 111299 Oncology and Carcinogenesis not elsewhere classified
Abstract

Supplemental material, Supplementary_methods_1 for Identification of an immune-suppressed subtype of feline triple-negative basal-like invasive mammary carcinomas, spontaneous models of breast cancer by Elie Dagher, Laura Simbault, Jérôme Abadie, Delphine Loussouarn, Mario Campone and Frédérique Nguyen in Tumor Biology

Published
2020
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14. SPECT-CT Imaging of Dog Spontaneous Diffuse Large B-Cell Lymphoma Targeting CD22 for the Implementation of a Relevant Preclinical Model for Human

Author

Etienne, Floriane, Berthaud, Maxime, Nguyen, Frédérique, Bernardeau, Karine, Maurel, Catherine, Bodet-Milin, Caroline, Diab, Maya, Abadie, Jérôme, Gouilleux-Gruart, Valérie, Vidal, Aurélien, Bourgeois, Mickael, Chouin, Nicolas, Ibisch, Catherine, Davodeau, François, Oncologie nucléaire (CRCINA - Département NOHMAD - Equipe 13), Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers (CRCINA), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Animaux Modèles pour la Recherche en Oncologie [Nantes] (AMaROC), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Stress Adaptation and Tumor Escape in breast cancer - SATE (CRCINA - Département ONCO - Equipe 8), Plateforme 'Production de protéines recombinantes' (P2R - INSERM UMS016/CNRS UMS3556/UN FED4203), Structure fédérative de recherche François Bonamy (SFR François Bonamy), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Santé de l'Université de Nantes (IRS-UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Santé de l'Université de Nantes (IRS-UN), Service de Médecine Nucléaire [Nantes], Hôpital Laennec, Groupe innovation et ciblage cellulaire (GICC), EA 7501 [2018-...] (GICC EA 7501), Université de Tours, Cyclotron ARRONAX [Saint-Herblain], ARRONAX - (GIP) Groupement d'Intérêt Public [Saint-Herblain] (Institut de Recherche Public), This work was supported by the Institut Thématique MultiOrganismes (ITMO) Cancer of the Alliance pour les sciences de la vie et de la santé (AVIESAN) jointly with the Institut National du Cancer (INCA) under one grant entitled: Spontaneous tumor models in animals for translational research in oncology no. A11196NS (CANIMAB). Financial support was also provided by a Physics, Mathematics and Engineering sciences applied to the Cancer Research grant (DogPPK project) awarded by INSERM and INCa., Nuclear Oncology (CRCINA-ÉQUIPE 13), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Animaux modèles pour la recherche en oncologie comparée (AMaROC), Stress Adaptation and Tumor Escape in Breast Cancer (CRCINA-ÉQUIPE 8), Université de Tours (UT), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), and Bernardo, Elizabeth

Subjects
comparative oncology, diffuse large B-cell lymphoma, [SDV.CAN]Life Sciences [q-bio]/Cancer, SPECT-CT imaging, internalization, [SDV.CAN] Life Sciences [q-bio]/Cancer, Oncology, immune system diseases, monoclonal antibody, hemic and lymphatic diseases, dog, internalization Etienne et al Radiolabeled Anti-canine CD22 Antibodies, CD22, Original Research
Abstract

International audience; Antibodies directed against CD22 have been used in radioimmunotherapy (RIT) clinical trials to treat patients with diffuse large B-cell lymphoma (DLBCL) with promising results. However, relevant preclinical models are needed to facilitate the evaluation and optimization of new protocols. Spontaneous DLBCL in dogs is a tumor model that may help accelerate the development of new methodologies and therapeutic strategies for RIT targeting CD22. Seven murine monoclonal antibodies specific for canine CD22 were produced by the hybridoma method and characterized. The antibodies' affinity and epitopic maps, their internalization capability and usefulness for diagnosis in immunohistochemistry were determined. Biodistribution and PET imaging on a mouse xenogeneic model of dog DLBCL was used to choose the most promising antibody for our purposes. PET-CT results confirmed biodistribution study observations and allowed tumor localization. The selected antibody, 10C6, was successfully used on a dog with spontaneous DLBCL for SPECT-CT imaging in the context of disease staging, validating its efficacy for diagnosis and the feasibility of future RIT assays. This first attempt at phenotypic imaging on dogs paves the way to implementing quantitative imaging methodologies that would be transposable to humans in a theranostic approach. Taking into account the feedback of existing human radioimmunotherapy clinical trials targeting CD22, animal trials are planned to investigate protocol improvements that are difficult to consider in humans due to ethical concerns.

Published
2020

21. One‐year conditional survival of dogs and cats with invasive mammary carcinomas: A concept inspired from human breast cancer.

Author

Chocteau, Florian, Mordelet, Valentin, Dagher, Elie, Loussouarn, Delphine, Abadie, Jérôme, and Nguyen, Frédérique

Subjects
BREAST cancer, CATS, PROGNOSIS, INVASIVE diagnosis, EARLY death, FELIDAE, DOGS
Abstract

Numerous studies have described the prognostic factors of canine and feline mammary carcinomas (MCs), that is, variables that predict patient survival after diagnosis. But how does survival estimation evolve in patients that escaped early death from their cancer? In human oncology, conditional survival (CS), the probability of surviving X further years when cancer patients have already survived Y years, is used to analyse cancer outcomes in a long‐term perspective. In this cohort of 344 dogs and 342 cats with surgically removed stage I to III invasive MCs, with a minimal follow‐up of 2 years, we calculated the 1‐year CS, that is, the probability for patients that have survived 1 year, to survive or to die from cancer during the subsequent year. The 1‐year conditional specific survival probabilities were 59% and 48% at diagnosis of invasive MC respectively in dogs and cats, and 80% and 52% in 1‐year surviving dogs and cats respectively, suggesting that 1‐year surviving dogs were relatively protected from cancer‐related death, whereas feline MCs remained life‐threatening cancers for longer periods of time. Among the most significant parameters associated with CS in surviving dogs and cats were the nodal stage and lymphovascular invasion, as well as patient age, cancer stage and margin status in surviving dogs. By comparison, tumour size and the histological grade did not significantly alter CS probabilities in surviving dogs and cats. Conditional survival may be considered a very interesting tool for veterinary practitioners to estimate the likely outcome of cancer survivors. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Production and characterization of monoclonal antibodies specific for canine CD138 (syndecan-1) for nuclear medicine preclinical trials on spontaneous tumours

Author

Diab, Maya, Nguyen, Frédérique, Berthaud, Maxime, Maurel, Catherine, Gaschet, J., Verger, Elise, Ibisch, Catherine, Chérel, Michel, Abadie, Jérôme, Davodeau, François, Rousseau, Caroline, Bernardo, Elizabeth, Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut de Cancérologie de l'Ouest [Angers/Nantes] (UNICANCER/ICO), UNICANCER, and Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
Mammary Neoplasms, Animal, [SDV.CAN]Life Sciences [q-bio]/Cancer, Mice, Dogs, breast cancer, [SDV.CAN] Life Sciences [q-bio]/Cancer, immune system diseases, hemic and lymphatic diseases, Animals, Humans, Dog Diseases, Mice, Inbred BALB C, Hybridomas, B-cell lymphoma, comparative, Antibodies, Monoclonal, syndecan-1, Radioimmunotherapy, Flow Cytometry, monoclonal antibody, dog, oncology, Female, Lymphoma, Large B-Cell, Diffuse, Epitope Mapping, diffuse large
Abstract

International audience; We isolated 11 antibodies specific for canine CD138 (cCD138) to validate the interest of CD138 antigen targeting in dogs with spontaneous mammary carcinoma. The affinity of the monoclonal antibodies in the nanomolar range is suitable for immunohistochemistry and nuclear medicine applications. Four distinct epitopes were recognized on cCD138 by this panel of antibodies. CD138 expression in canine healthy tissues is comparable to that reported in humans. CD138 is frequently expressed in canine mammary carcinomas corresponding to the human triple negative breast cancer subtype, with cytoplasmic and membranous expression. In canine diffuse large B-cell lymphoma, CD138 expression is associated with the 'non-germinal center' phenotype corresponding to the most aggressive subtype in humans. This homology of CD138 expression between dogs and humans confirms the relevance of tumour-bearing dogs as spontaneous models for nuclear medicine applications, especially for the evaluation of new tumour targeting strategies for diagnosis by phenotypic imaging and radio-immunotherapy.

Published
2016

26. Additional file 2: Table S2. of The dog as a naturally-occurring model for insulin-like growth factor type 1 receptor-overexpressing breast cancer: an observational cohort study

Author

Jaillardon, Laetitia, Jérome Abadie, Godard, Tiffanie, Campone, Mario, Loussouarn, Delphine, Siliart, Brigitte, and Nguyen, Frédérique

Subjects
body regions
Abstract

Significant associations between IGF1R expression and clinicopathological features of 47 luminal canine mammary carcinomas. IGF1R score 0–1+ is considered as the reference for each parameter. IGF1R Insulin-like Growth Factor type 1 Receptor. LVI: Lymphovascular Invasion. OR: Odd Ratio. 95 % CI: 95 % Confidence Interval. (DOC 30 kb)

Published
2015
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27. Additional file 1: Table S1. of The dog as a naturally-occurring model for insulin-like growth factor type 1 receptor-overexpressing breast cancer: an observational cohort study

Author

Jaillardon, Laetitia, Jérome Abadie, Godard, Tiffanie, Campone, Mario, Loussouarn, Delphine, Siliart, Brigitte, and Nguyen, Frédérique

Abstract

Primary antibodies and immunohistochemical protocols (Benchmark XT Ventana, Roche Diagnostics). All dilutions were performed using a commercially available diluent (Ventana Medical Systems). aUltraview and bOptiview Universal DAB detection kit: multimer-technology based detection system. cIView Universal DAB detection kit: biotin streptavidin system. ERα: Estrogen Receptor alpha, PR: Progesterone Receptor, HER2: Epidermal Growth Factor type 2 Receptor, CK5/6: Cytokeratin 5/6, EGFR: Epidermal Growth Factor type 1 Receptor, IGF1R: Insulin-like growth factor type 1 receptor. CC1: Cell Conditioning 1. (DOC 33 kb)

Published
2015
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29. Canine invasive mammary carcinomas as models of human breast cancer. Part 1: natural history and prognostic factors.

Author

Nguyen, Frédérique, Peña, Laura, Ibisch, Catherine, Loussouarn, Delphine, Gama, Adelina, Rieder, Natascha, Belousov, Anton, Campone, Mario, and Abadie, Jérôme

Abstract

Purpose: Dogs have been proposed as spontaneous animal models of human breast cancer, based on clinicopathologic similarities between canine and human mammary carcinomas. We hypothesized that a better knowledge of the natural history and prognostic factors of canine invasive mammary carcinomas would favor the design of preclinical trials using dogs as models of breast cancer.Methods: The 2-year outcome of 350 female dogs with spontaneous invasive mammary carcinoma was studied. The investigated prognostic factors included age at diagnosis, pathologic tumor size, pathologic nodal stage, lymphovascular invasion, histological grade, and expression of Estrogen Receptor alpha (ERα), Progesterone Receptor, Ki-67, Human Epidermal Growth Factor Receptor 2, basal cytokeratins 5/6, and Epidermal Growth Factor Receptor. Multivariate survival analyses were performed using the Cox proportional hazards model.Results: The overall survival after mastectomy was 11 months. Within 1 year post mastectomy, 41.5% of dogs (145/350) died from their mammary carcinoma. By multivariate analysis, the significant prognostic factors for overall survival included a pathologic tumor size larger than 20 mm [HR 1.47 (95% confidence interval 1.15–1.89)], a positive nodal stage [pN+, HR 1.89 (1.43–2.48)], a histological grade III [HR 1.32 (1.02–1.69)], ERα negativity [HR 1.39 (1.01–1.89)], a high Ki-67 proliferation index [HR 1.32 (1.04–1.67)], and EGFR absence [HR 1.33 (1.04–1.69)].Conclusion: The short natural history of spontaneous canine invasive mammary carcinomas and high rate of cancer-related death allow for rapid termination of preclinical investigations. The prognostic factors of invasive mammary carcinomas are remarkably similar in dogs and humans, highlighting the similarities in cancer biology between both species. [ABSTRACT FROM AUTHOR]

Published
2018
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30. Canine invasive mammary carcinomas as models of human breast cancer. Part 2: immunophenotypes and prognostic significance.

Author

Abadie, Jérôme, Nguyen, Frédérique, Loussouarn, Delphine, Peña, Laura, Gama, Adelina, Rieder, Natascha, Belousov, Anton, Bemelmans, Ingrid, Jaillardon, Laëtitia, Ibisch, Catherine, and Campone, Mario

Abstract

Purpose: Relevant animal models of human breast cancer are currently needed, especially for the aggressive triple-negative breast cancer subtype. Recent studies and our results (Part 1) indicate that spontaneous canine invasive mammary carcinomas (CMCs) resemble human breast cancer by clinics and pathology as well as behavior and prognostic indicators. We hypothesized that the current molecular classifications of human breast cancer, used for therapeutic decision, could be relevant to dogs.Methods: Three hundred and fifty female dogs with spontaneous CMC and a 2-year follow-up were retrospectively included. By immunohistochemistry, CMCs were classified according to Nielsen (Clin Cancer Res 10:5367–5374, 2004) and Blows (PlosOne doi: 10.1371/journal.pmed.1000279, 2010) into the subtypes of human breast cancer.Results: Four immunophenotypes were defined either according to Nielsen classification (luminal A 14.3%, luminal B 9.4%, triple-negative basal-like 58.6%, and triple-negative nonbasal-like 17.7% CMCs); or to Blows classification (luminal 1−: 11.4%, luminal 1+: 12.3%, Core basal phenotype: 58.6%, and five-negative phenotype: 17.7%). No HER2-overexpressing CMC as defined by a 3 + immunohistochemical score was observed in our cohort. By univariate and multivariate analyses, both immunophenotypical classifications applied to CMCs showed strong prognostic significance: luminal A or luminal 1+ CMCs showed a significantly longer disease-free interval (HR = 0.46), Overall (HR = 0.47), and Specific Survival (HR = 0.56) compared to triple-negative carcinomas, after adjustment for stage.Conclusions: In our cohort, triple-negative CMCs largely predominated (76%), were much more prevalent than in human beings, and showed an aggressive natural behavior after mastectomy. Dogs are thus potent valuable spontaneous models to test new therapeutic strategies for this particular subtype of breast cancer. [ABSTRACT FROM AUTHOR]

Published
2018
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32. Prognostic significance of microvascular density in canine cutaneous melanoma

Author

Wolfe-Taylor Boedec, K, Nguyen, Frédérique, Izembart, A, Guigand, Lydie, Wyers, Monique, Abadie, J, Inconnu, Développement et Pathologie du Tissu Musculaire (DPTM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2007

33. High frequency of spontaneous rhabdomyosarcomas in older mdx mice with X-linked muscular dystrophy

Author

Nguyen, Frédérique, Dorso, L, Belluco, S, Rouger, Karl, Fernandez, Bernard-Yves, Guéreaud, Catherine, Guigand, Lydie, Wyers, Monique, Cherel, Yan, Inconnu, Développement et Pathologie du Tissu Musculaire (DPTM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2007

34. Correlations between Magnetic Resonance Imaging and histopathology in the mdx (X-linked muscular dystrophy) murine model, of Duchenne Muscular Dystrophy

Author

Nguyen, Frédérique, Eliat, PA, Pinot, Marie-Sophie, Franconi, Florence, Lemaire, Laura, de Certaines, J, Cherel, Yan, ProdInra, Migration, Inconnu, Développement et Pathologie du Tissu Musculaire (DPTM), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2006

35. Microvasculature in skeletal muscles of Golden Retriever Muscular Dystrophy dogs

Author

Nguyen, Frédérique, Masson, Mt, Guigand, Lydie, Goubault-Leroux, I, Lavault, Mt, Primaut, R, Wyers, Monique, Cherel, Yan, Inconnu, Développement et Pathologie du Tissu Musculaire (DPTM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2005

36. Les lésions musculaires des modèles animaux de la dystrophie musculaire de Duchenne : évaluation comparée des lésions du modèle murin mdx (X-linked muscular dystrophy) par histopathologie et analyse de texture en IRM : étude de la vascularisation capillaire du muscle canin GRMD (Golden Retriever Muscular Dystrophy)

Author

Nguyen, Frédérique and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], IMAGERIE PAR RÉSONANCE MAGNÉTIQUE, these
Abstract

La dystrophie musculaire de Duchenne (DMD) est une dystrophie musculaire fatale de l'enfant, due à l'absence de dystrophine, protéine du cytosquelette des myofibres. Dans le modèle murin mdx de la DMD, les lésions musculaires d'anisocytose, conversion oxydative des fibres, nécrose et régénération peuvent être quantifiées par analyse de texture en IRM, méthode non invasive proposée pour le suivi d'essais thérapeutiques entrepris dans ce modèle. Dans le modèle canin GRMD, la forme néonatale fulminante est un modèle des lésions précoces de dystrophinopathie, et la forme classique observée à partir de l'âge de deux mois un excellent modèle lésionnel de la DMD. Le réseau capillaire du muscle GRMD est quantitativement peu modifié par rapport aux témoins. La structure des capillaires est modifiée par duplication de la membrane basale qui, avec la fibrose endomysiale précoce, représente un obstacle physique à la diffusion d'un produit thérapeutique génique ou cellulaire.

Published
2005

37. Correlations between Magnetic Resonance Imaging texture features at 7T and histopathology in mdx mice muscles

Author

Eliat, PA, Nguyen, Frédérique, Pinot, Marie-Sophie, Franconi, Florence, Lemaire, Laura, De Certaines, JD, Cherel, Yan, ProdInra, Migration, Inconnu, Développement et Pathologie du Tissu Musculaire (DPTM), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2005

41. Comparative analysis of BRAF, NRAS and c- KIT mutation status between tumor tissues and autologous tumor cell-lines of stage III/ IV melanoma.

Author

Knol, Anne ‐ Chantal, Pandolfino, Marie ‐ Christine, Vallée, Audrey, Nguyen, Frédérique, Lella, Virginie, Khammari, Amir, Denis, Marc, Puaux, Anne ‐ Laure, and Dréno, Brigitte

Subjects
MELANOMA, GENETIC mutation, MELANOMA treatment, TUMORS, IMMUNOHISTOCHEMISTRY, PROTEIN expression
Abstract

In the last decade, advances in molecular biology have provided evidence of the genotypic heterogeneity of melanoma. We analysed BRAF, NRAS and c- KIT alterations in tissue samples from 63 stage III/ IV melanoma patients and autologous cell-lines, using either allele-specific or quantitative PCR. The expression of BRAF V600E protein was also investigated using an anti- BRAF antibody in the same tissue samples. 81% of FFPE samples and tumor cell-lines harboured a genetic alteration in either BRAF (54%) or NRAS (27%) oncogenes. There was a strong concordance (100%) between tissue samples and tumor cell-lines. The BRAF V600E mutant-specific antibody showed high sensitivity (96%) and specificity (100%) for detecting the presence of a BRAF V600E mutation. The correlation was of 98% between PCR and immunohistochemistry results for BRAF mutation. These results suggest that BRAF and NRAS mutation status of tumor cells is not affected by culture conditions. [ABSTRACT FROM AUTHOR]

Published
2015
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44. Synthesis and characterization of supramolecular biovector (SMBV) specifically designed for the entrapment of ionic molecules

Author

De Miguel, Ignacio, primary, Ioualalena, Karim, additional, Bonnefous, Monique, additional, Peyrot, Marianne, additional, Nguyen, Frédérique, additional, Cervilla, Monique, additional, Soulet, Nadine, additional, Dirson, Roselyne, additional, Rieumajou, Valérie, additional, Imbertie, Laurent, additional, Solers, Corinne, additional, Cazes, Sylvie, additional, Favre, Gilles, additional, and Samain, Daniel, additional

Published
1995
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