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Production and characterization of monoclonal antibodies specific for canine CD138 (syndecan-1) for nuclear medicine preclinical trials on spontaneous tumours
- Source :
- Veterinary and Comparative Oncology, Veterinary and Comparative Oncology, 2016, [Epub ahead of print]. ⟨10.1111/vco.12233⟩, Veterinary and Comparative Oncology, Wiley, 2016, [Epub ahead of print]. ⟨10.1111/vco.12233⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; We isolated 11 antibodies specific for canine CD138 (cCD138) to validate the interest of CD138 antigen targeting in dogs with spontaneous mammary carcinoma. The affinity of the monoclonal antibodies in the nanomolar range is suitable for immunohistochemistry and nuclear medicine applications. Four distinct epitopes were recognized on cCD138 by this panel of antibodies. CD138 expression in canine healthy tissues is comparable to that reported in humans. CD138 is frequently expressed in canine mammary carcinomas corresponding to the human triple negative breast cancer subtype, with cytoplasmic and membranous expression. In canine diffuse large B-cell lymphoma, CD138 expression is associated with the 'non-germinal center' phenotype corresponding to the most aggressive subtype in humans. This homology of CD138 expression between dogs and humans confirms the relevance of tumour-bearing dogs as spontaneous models for nuclear medicine applications, especially for the evaluation of new tumour targeting strategies for diagnosis by phenotypic imaging and radio-immunotherapy.
- Subjects :
- Mammary Neoplasms, Animal
[SDV.CAN]Life Sciences [q-bio]/Cancer
Mice
Dogs
breast cancer
[SDV.CAN] Life Sciences [q-bio]/Cancer
immune system diseases
hemic and lymphatic diseases
Animals
Humans
Dog Diseases
Mice, Inbred BALB C
Hybridomas
B-cell lymphoma
comparative
Antibodies, Monoclonal
syndecan-1
Radioimmunotherapy
Flow Cytometry
monoclonal antibody
dog
oncology
Female
Lymphoma, Large B-Cell, Diffuse
Epitope Mapping
diffuse large
Subjects
Details
- Language :
- English
- ISSN :
- 14765810 and 14765829
- Database :
- OpenAIRE
- Journal :
- Veterinary and Comparative Oncology, Veterinary and Comparative Oncology, 2016, [Epub ahead of print]. ⟨10.1111/vco.12233⟩, Veterinary and Comparative Oncology, Wiley, 2016, [Epub ahead of print]. ⟨10.1111/vco.12233⟩
- Accession number :
- edsair.pmid.dedup....d76869a439f41b7f2c2b59dcb9ddabb7