34 results on '"Naydan DK"'
Search Results
2. The expression of calretinin and cytokeratins in canine acanthomatous ameloblastoma and oral squamous cell carcinoma.
- Author
-
Fulton A, Arzi B, Murphy B, Naydan DK, and Verstraete FJ
- Subjects
- Ameloblastoma metabolism, Ameloblastoma pathology, Animals, Antibodies, Monoclonal, California, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Dog Diseases pathology, Dogs, Immunohistochemistry veterinary, Jaw Neoplasms metabolism, Jaw Neoplasms pathology, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, Tooth pathology, Universities, Ameloblastoma veterinary, Calbindin 2 metabolism, Carcinoma, Squamous Cell veterinary, Dog Diseases metabolism, Jaw Neoplasms veterinary, Keratins metabolism, Mouth Neoplasms veterinary
- Abstract
Oral squamous cell carcinoma (OSCC) and canine acanthomatous ameloblastoma (CAA) represent two epithelium-derived neoplasms that affect the oral cavity of dogs. The expression of cytokeratins (CKs) and calretinin has been previously established in the canine tooth bud and odontogenic tumours. The aim of this study was to characterize the CK and calretinin expression profile of OSCC in comparison to CAA and canine tooth bud tissues. Samples from 15 OSCC and 15 CAA cases, as well as 6 tooth buds and 2 normal gingival tissues were examined. OSCC CK expression was consistent with the CK expression profile of CAA and canine tooth bud tissue. Calretinin was positively expressed in 10 of 15 OSCC cases, with 5 cases demonstrating high staining intensity. Only 2 of 15 CAA cases demonstrated mild-moderate staining intensity. The statistically significant difference in staining pattern and intensity of calretinin in OSCC and CAA can help distinguish between these two tumour types., (© 2012 Blackwell Publishing Ltd.)
- Published
- 2014
- Full Text
- View/download PDF
3. Benign gastric neuroendocrine tumors in three snow leopards (Panthera uncia).
- Author
-
Dobson EC, Naydan DK, Raphael BL, and McAloose D
- Subjects
- Animals, Female, Male, Neuroendocrine Tumors pathology, Stomach Neoplasms pathology, Neuroendocrine Tumors veterinary, Panthera, Stomach Neoplasms veterinary
- Abstract
Neuroendocrine tumors are relatively rare neoplasms arising from neuroendocrine cells that are distributed throughout the body and are predominant in the gastrointestinal tract. This report describes benign, well-differentiated gastric neuroendocrine tumors in three captive snow leopards (Panthera uncia). All tumors were well circumscribed, were within the gastric mucosa or submucosa, and had histologic and immunohistochemical features of neuroendocrine tumors. Histologic features included packeted cuboidal to columnar epithelial cells that were arranged in palisades or pseudorosettes and contained finely granular cellular cytoplasm with centrally placed, round nuclei. Cytoplasmic granules of neoplastic cells strongly expressed chromogranin A, variably expressed neuron-specific enolase, and did not express synaptophysin or gastrin. Each leopard died or was euthanatized for reasons unrelated to its tumor.
- Published
- 2013
- Full Text
- View/download PDF
4. Immunohistochemical characteristics of normal canine eyes.
- Author
-
Labelle P, Reilly CM, Naydan DK, and Labelle AL
- Subjects
- Animals, Biomarkers analysis, Cornea cytology, Cornea metabolism, Cytoskeletal Proteins analysis, Cytoskeletal Proteins metabolism, Dog Diseases immunology, Dog Diseases pathology, Eye cytology, Eye Diseases diagnosis, Eye Diseases immunology, Immunophenotyping veterinary, Male, Reference Values, Biomarkers metabolism, Dog Diseases diagnosis, Dogs anatomy & histology, Eye metabolism, Eye Diseases veterinary, Immunohistochemistry veterinary
- Abstract
Immunohistochemistry is widely utilized in diagnostic laboratories to study neoplastic and nonneoplastic diseases. Knowledge of the immunohistochemical characteristics of normal tissue is essential for interpretation of immunoreactivity in pathologic conditions. In this study, immunohistochemistry was performed with a broad panel of diagnostically relevant antibodies on 4 normal canine globes--namely, vimentin, pan-cytokeratin (AE1/AE3), cytokeratin 7, cytokeratin 8/18, cytokeratin 20, α-smooth muscle actin, muscle specific actin, desmin, Melan-A, microphthalmia transcription factor, S-100, glial fibrillary acidic protein, triple neurofilaments, neuron-specific enolase, chromogranin A, synaptophysin, laminin and CD31. Results include cytokeratin immunoreactivity limited to the conjunctival epithelium, corneal epithelium, and retinal pigment epithelium; distinct patterns of immunopositivity of muscle markers; and widespread immunoreactivity for vimentin and most neural/neuroendocrine markers. These findings in normal eyes provide the basis for interpretation of ocular immunohistochemistry in dogs. Published immunophenotypes of primary ocular neoplasms are also reviewed.
- Published
- 2012
- Full Text
- View/download PDF
5. Expression of cytokeratins in the epithelium of canine odontogenic tumours.
- Author
-
Arzi B, Murphy B, Nemec A, Vapniarsky N, Naydan DK, and Verstraete FJ
- Subjects
- Ameloblastoma genetics, Ameloblastoma metabolism, Ameloblastoma pathology, Ameloblastoma veterinary, Animals, Cell Differentiation, Dog Diseases genetics, Dog Diseases pathology, Dogs, Fibroma genetics, Fibroma metabolism, Fibroma pathology, Fibroma veterinary, Gingiva metabolism, Gingival Neoplasms genetics, Gingival Neoplasms metabolism, Gingival Neoplasms pathology, Intermediate Filaments metabolism, Keratins genetics, Neoplasm Proteins genetics, Odontogenic Tumors genetics, Odontogenic Tumors metabolism, Odontogenic Tumors pathology, Odontoma genetics, Odontoma metabolism, Odontoma pathology, Odontoma veterinary, Tooth Germ metabolism, Dog Diseases metabolism, Epithelium metabolism, Gene Expression Regulation, Neoplastic, Gingival Neoplasms veterinary, Keratins biosynthesis, Neoplasm Proteins biosynthesis, Odontogenic Tumors veterinary
- Abstract
Odontogenic tumours are considered to be relatively rare; however, several histologically distinct types have been identified in dogs. The more common canine odontogenic tumours are peripheral odontogenic fibroma and canine acanthomatous ameloblastoma. The expression of cytokeratins (CKs) has been established for the human dental germ and odontogenic tumours. The aim of the present study was to describe the immunohistochemical expression of a panel of CKs in the epithelium of the canine dental germ, normal gingiva and odontogenic tumours arising in this species. Samples from 20 odontogenic tumours, 12 tooth germs and three normal gingival tissues were obtained. Each sample was stained with haematoxylin and eosin and subjected to immunohistochemistry for CK expression. The typical expression pattern of CKs in the odontogenic epithelium and gingiva of dogs was CK14 and CK5/6. CKs 7, 8, 18 and 20 were generally absent from the canine dental germ, gingiva and odontogenic tumours. Dogs and man therefore exhibit similar CK expression in the odontogenic epithelium., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
6. Expression of cyclooxygenase genes in the jejunum of horses during low-flow ischemia and reperfusion.
- Author
-
Hilton H, Nieto JE, Moore PF, Harmon FA, Naydan DK, and Snyder JR
- Subjects
- Animals, Female, Gene Expression Regulation, Enzymologic, Horse Diseases metabolism, Horses, Ischemia metabolism, Jejunal Diseases metabolism, Jejunal Diseases pathology, Jejunum metabolism, Jejunum pathology, Male, Reperfusion Injury metabolism, Up-Regulation, Cyclooxygenase 1 genetics, Cyclooxygenase 2 genetics, Horse Diseases pathology, Ischemia veterinary, Jejunal Diseases veterinary, Jejunum blood supply, Reperfusion Injury veterinary
- Abstract
Objective: To determine expression of cyclooxygenase (COX) genes 1 and 2 (also called prostaglandin-endoperoxide synthases 1 and 2) and stability of housekeeping gene expression during low-flow ischemia and reperfusion in the jejunum of horses., Animals: 5 healthy adult horses., Procedures: Horses were anesthetized, and two 30-cm segments of jejunum were surgically exteriorized. Blood flow was maintained at baseline (untreated) values in 1 (control) segment and was decreased to 20% of baseline (low-flow ischemia) for 75 minutes, followed by 75 minutes of reperfusion, in the other (experimental) segment. Biopsy samples were collected from experimental segments at baseline (T0), after 75 minutes of ischemia (T1), and after 75 minutes of reperfusion (T2); samples were collected from control segments at T0 and T2. Horses were euthanized 24 hours after induction of ischemia (T3), and additional samples were collected. Samples were evaluated histologically. Total RNA was extracted; expression of COX genes and stability of 8 housekeeping genes were determined via quantitative real-time PCR assays., Results: COX-1 and COX-2 genes were constitutively expressed in baseline samples. Low-flow ischemia resulted in significant upregulation of COX-2 gene expression at each subsequent time point, compared with baseline values. The most stably expressed reference genes were β-actin and hypoxanthine phosphoribosyltransferase, whereas glyceraldehyde 3-phosphate dehydrogenase and β-2 microglobulin were the least stably expressed., Conclusions and Clinical Relevance: Low-flow ischemia resulted in upregulation of COX-2 gene expression in the jejunum of horses. Housekeeping genes traditionally used as internal standards may not be stable in this tissue during arterial low-flow ischemia and reperfusion.
- Published
- 2011
- Full Text
- View/download PDF
7. Analysis of immune cells within the healthy oral mucosa of specific pathogen-free cats.
- Author
-
Arzi B, Murphy B, Baumgarth N, Vapniarsky N, Nemec A, Naydan DK, Cox DP, and Verstraete FJ
- Subjects
- Animals, Antigens, CD, B-Lymphocytes immunology, Female, Fluorescent Antibody Technique, Immunophenotyping, Male, Specific Pathogen-Free Organisms, Cats immunology, Langerhans Cells immunology, Lymphocyte Subsets immunology, Macrophages immunology, Mast Cells immunology, Mouth Mucosa immunology
- Abstract
The oral mucosa is an important interface for host-environment interactions. Based on previous studies, it is generally accepted that the cellular compartments of the oral immune system comprise organized mucosal-associated lymphoid tissues as well as diffusely and focally distributed T- and to lesser extent B-lymphocytes, oral mucosal Langerhans cells (OMLC), macrophages and mast cells. However, a comprehensive quantification of the cellular elements in the oral mucous membranes of the cat has not been reported. The aim of this study was to provide a comprehensive analysis of the immune cell compartments in the oral mucous membranes and anatomically related tissues of healthy cats. Multiple biopsies of the oral mucous membranes and related tissues were obtained from four specific pathogen-free cats for histological and immunohistochemical assessment of lymphocyte subsets, OMLC, macrophages and mast cells. T-lymphocyte subsets, OMLC, mast cells and macrophages were present in varying frequencies among the tissue compartments of the feline oral cavity. B-lymphocytes were not identified in any of the examined tissues except the tonsils and mandibular lymph nodes. Lymphocytic aggregates (follicles) were found in the palatoglossal folds and the gingiva. We describe the topographical distribution of various leucocyte subsets in the normal healthy feline oral mucosa, and demonstrate regional differences in the distribution of these cells.
- Published
- 2011
- Full Text
- View/download PDF
8. Normal morphology and hormone receptor expression in the male California sea lion (Zalophus californianus) genital tract.
- Author
-
Colegrove KM, Gulland FM, Naydan DK, and Lowenstine LJ
- Subjects
- Animals, Estrogens metabolism, Genitalia, Male metabolism, Immunohistochemistry, Male, Penis cytology, Penis metabolism, Progesterone metabolism, Sea Lions physiology, Sex Differentiation physiology, Genitalia, Male anatomy & histology, Gonadal Steroid Hormones metabolism, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Sea Lions anatomy & histology
- Abstract
Histomorphology and estrogen alpha (ER alpha), and progesterone receptor (PR) expression were evaluated in free-ranging stranded male California sea lions (Zalophus californianus). Hormone receptor expression was evaluated using an immunohistochemical technique with monoclonal antibodies. Estrogen and PRs were identified in the efferent ductules, prostate gland, corpus cavernosa, corpus spongiosium, penile urethra, and in the epithelium and stroma of both the penis and prepuce. In some tissues, ER alpha expression was more intense in the stroma, emphasizing the importance of the stroma in hormone-mediated growth and differentiation of reproductive organs. To our knowledge, this is the first study to localize ER alpha and PR to the epithelium of the glans penis. The results of this investigation add to the general knowledge of male California sea lion reproduction and suggest that estrogens could have a role in the function of the male reproductive tract.
- Published
- 2009
- Full Text
- View/download PDF
9. The normal genital tract of the female California sea lion (Zalophus californianus): cyclic changes in histomorphology and hormone receptor distribution.
- Author
-
Colegrove KM, Gulland FM, Naydan DK, and Lowenstine LJ
- Subjects
- Animals, Corpus Luteum cytology, Corpus Luteum metabolism, Endometrium cytology, Endometrium metabolism, Epithelial Cells cytology, Epithelial Cells metabolism, Female, Genitalia, Female metabolism, Ovarian Follicle cytology, Ovarian Follicle metabolism, Ovary cytology, Ovary metabolism, Sea Lions physiology, Seasons, Species Specificity, Stromal Cells cytology, Stromal Cells metabolism, Estrous Cycle physiology, Genitalia, Female cytology, Gonadal Steroid Hormones metabolism, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Sea Lions anatomy & histology
- Abstract
Changes in reproductive tract histomorphology, and estrogen (ERalpha) and progesterone receptor (PR) expression throughout the breeding cycle were evaluated in free-ranging stranded female California sea lions (Zalophus californianus). Hormone receptor expression in the ovaries, uterus, cervix, and vagina was evaluated using an immunohistochemical technique with monoclonal antibodies. During a large portion of the cycle, ovaries contained both a corpora lutea (CL) and follicles in varying stages of development. In the periods of pupping and estrus during June and July, and in the spring morphologic features of the endometrium suggested estrogen influence. There were areas of squamous differentiation in the pseudostratified columnar epithelium of the cervix and vagina in some animals during estrus and in the spring. Estrogen receptor immunohistochemical scores were highest during pupping and estrus and in the spring and lowest during embryonic diapause. Cyclic changes in uterine PR expression throughout the cycle were minimal. Both ERalpha and PR were expressed in epithelial and stromal cells throughout the cervix and vagina, however, receptor expression was typically higher in the stroma. Stromal cell hormone receptors may play an important role in epithelial responses to circulating sex hormones. The results of this investigation add to the general knowledge of California sea lion reproduction and establish baseline information on reproductive tract hormone receptors that will aid in determining the factors involved in urogenital cancer development in sea lions.
- Published
- 2009
- Full Text
- View/download PDF
10. Tumor morphology and immunohistochemical expression of estrogen receptor, progesterone receptor, p53, and Ki67 in urogenital carcinomas of California sea lions (Zalophus californianus).
- Author
-
Colegrove KM, Gulland FM, Naydan DK, and Lowenstine LJ
- Subjects
- Animals, California, Estrogen Receptor alpha metabolism, Immunohistochemistry veterinary, Ki-67 Antigen metabolism, Receptors, Progesterone metabolism, Statistics, Nonparametric, Tumor Suppressor Protein p53 metabolism, Carcinoma metabolism, Carcinoma pathology, Carcinoma veterinary, Sea Lions, Urogenital Neoplasms metabolism, Urogenital Neoplasms pathology, Urogenital Neoplasms veterinary
- Abstract
Metastatic carcinoma of urogenital origin is a common cause of mortality in free-ranging California sea lions (Zalophus californianus). The etiology of this cancer is likely multifactorial, with viral infection, genetic factors, and exposure to environmental organochlorine contaminants possible contributing factors. In this study, expression of estrogen receptor alpha (ER alpha), progesterone receptor (PR), p53, and Ki67 were evaluated by immunohistochemistry in 12 sea lions with metastatic carcinoma, genital epithelial dysplasia, and intraepithelial neoplasia; 4 with genital epithelial dysplasia and intraepithelial neoplasia without metastases; and 6 control animals. Dysplastic and neoplastic lesions were identified in multiple areas of the cervix, vagina, penis, prepuce, and urethra in affected animals, suggesting multicentric development. Lesions were graded according to degree of epithelial dysplasia and infiltration and lesions of different grades were evaluated separately. Estrogen receptor expression was lower in intraepithelial lesions compared with normal genital epithelium, and expression in metastatic lesions was completely absent. There was progesterone receptor expression in neoplastic cells in intraepithelial lesions of all grades and in metastases, with no significant difference between lesion grades or between control and affected epithelium. Ki67 index and p53 expression increased with lesion grade and were higher in lesions than normal epithelium. Metastatic tumors exhibited highly variable morphology; however, proliferation index, ER alpha, PR, and p53 expression were similar in tumors with different patterns of growth. These results suggest that endogenous hormones, environmental contaminants that interact with steroid hormone receptors, and alterations in p53 may play a role in urogenital carcinogenesis in California sea lions.
- Published
- 2009
- Full Text
- View/download PDF
11. Pathological features of amyloidosis in stranded California sea lions (Zalophus californianus).
- Author
-
Colegrove KM, Gulland FM, Harr K, Naydan DK, and Lowenstine LJ
- Subjects
- Amyloidosis metabolism, Animals, Immunohistochemistry, Kidney pathology, Serum Amyloid A Protein analysis, Serum Amyloid A Protein metabolism, Thyroid Gland pathology, Amyloidosis pathology, Amyloidosis veterinary, Sea Lions
- Abstract
Amyloidosis was diagnosed in 26 stranded adult California sea lions between 1983 and 2006 by retrospective case analysis. The kidneys (92.3% of animals), blood vessels (80.7%) and thyroid glands (65.4%) were most commonly affected. Macroscopically, affected kidneys were swollen, with pale tan cortices and loss of corticomedullary differentiation. Amyloid deposits in the kidney were located in the glomeruli, blood vessels, and peritubular interstitium, most prominently in the outer stripe of the medulla. The amyloid deposits were identified as type amyloid A (AA) by potassium permanganate staining and immunolabelling with antibodies against AA protein. Concurrent diseases, including inflammatory processes and genital carcinoma, were common in affected animals. Serum amyloid A concentrations were high (>1200 microg/ml) in six of seven affected sea lions, while the median value in clinically healthy animals was <10 microg/ml. These findings suggest that renal amyloidosis contributes to morbidity and mortality in stranded adult California sea lions.
- Published
- 2009
- Full Text
- View/download PDF
12. Histologic features of mammary carcinomas in zoo felids treated with melengestrol acetate (MGA) contraceptives.
- Author
-
McAloose D, Munson L, and Naydan DK
- Subjects
- Animals, Gene Expression Regulation, Neoplastic, Mammary Neoplasms, Animal pathology, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Retrospective Studies, Contraceptive Agents adverse effects, Mammary Neoplasms, Animal chemically induced, Melengestrol Acetate adverse effects, Panthera
- Abstract
Melengestrol acetate (MGA), a potent synthetic progestin, has been used as a contraceptive in zoo felids since 1975. Mammary gland carcinomas have been linked to MGA treatment in zoo felids, but the histologic features of these tumors and steroid receptor expression have not been described. Zoo felid mammary tumors were requested from participating zoos from 1986 through 1998, and 31 mammary carcinomas from 28 MGA-treated and 3 untreated felids were received. The carcinomas were evaluated on the basis of histologic pattern, tumor grade, and occurrence of metastasis; then features of the tumors were compared to determine if carcinomas in MGA-treated felids differed from those that occur spontaneously. Estrogen- and progesterone-receptor expression was evaluated in 17 of the 31 carcinomas. Of the 31 tumors, 22 (70.9%) had multiple histologic patterns, 29 (93.5%) were high grade, and 28 (90.3%) had metastasized. Within tumors, the tubulopapillary pattern was most common (87.1%, n = 27); solid (61.3%, n = 19), cribriform (38.7%, n = 12), and comedone (25.8%, n = 8) patterns were less common; and the mucinous (3.2%, n = 1) pattern was rare. Both MGA-treated and untreated zoo felids had similar patterns and grades of mammary gland cancer as well as prevalence of metastasis. These results indicate that mammary carcinomas in zoo felids are high grade with a predominant tubulopapillary pattern and aggressive behavior. Five of 17 carcinomas expressed progesterone receptors, and 1 of 17 expressed estrogen receptors. Although more zoo felids with cancer had been exposed to MGA in this study, mammary carcinomas were similar in appearance and behavior in untreated and MGA-treated zoo felids. The association of MGA with the development of malignant mammary gland tumors should be considered when using this contraceptive in zoo felids.
- Published
- 2007
- Full Text
- View/download PDF
13. Development of a new canine osteosarcoma cell line.
- Author
-
Séguin B, Zwerdling T, McCallan JL, DeCock HE, Dewe LL, Naydan DK, Young AE, Bannasch DL, Foreman O, and Kent MS
- Abstract
Establishing a canine osteosarcoma (OSA) cell line can be useful to develop in vivo and in vitro models of OSA. The goal of this study was to develop, characterize and authenticate a new canine OSA cell line and a clone. A cell line and a clone were developed with standard cell culture techniques from a naturally occurring OSA in a dog. The clonal cell line induced a tumour after injection in RAG 1-deficient mouse. Histology was consistent with OSA. The original tumour from the dog and the tumour induced in the mouse were both reactive with vimentin and osteonectin (ON). The parent cell line and clonal cell line were reactive with ON, osteocalcin and alkaline phosphatase. Loss of heterozygosity was found in the same three microsatellite markers in the parent and clonal cell lines, and the tumour tissue grown in the mouse.
- Published
- 2006
- Full Text
- View/download PDF
14. Interstitial lung disease in West Highland White Terriers.
- Author
-
Norris AJ, Naydan DK, and Wilson DW
- Subjects
- Animals, Collagen Type I metabolism, Collagen Type III metabolism, Dog Diseases metabolism, Dogs, Extracellular Matrix metabolism, Extracellular Matrix pathology, Extracellular Matrix ultrastructure, Female, Humans, Immunohistochemistry veterinary, Lung Diseases, Interstitial metabolism, Lung Diseases, Interstitial pathology, Male, Microscopy, Electron veterinary, Retrospective Studies, Dog Diseases pathology, Lung Diseases, Interstitial veterinary
- Abstract
Progressive respiratory failure and pulmonary fibrosis in West Highland White Terriers (WHWT) is an apparently genetic disorder of unknown pathogenesis. This study characterizes the light microscopic, ultrastructural, and immunohistochemical features of affected WHWT in comparison with lesions in usual interstitial pneumonia (UIP) of humans. Lesions in WHWT were confined to the expansion of the interstitial space of alveolar septa by extracellular matrix (ECM) determined to be mixtures of type-I and -III collagens. Features of UIP such as intra-alveolar fibroblastic foci, subpleural distribution, and honeycombing were not observed in six WHWT. Comparison with normal dogs showed no apparent increase in septal myofibroblasts. Ultrastructually, the ECM in alveolar septa consisted of large aggregates of periodic collagen filaments underlying alveolar capillaries that were surrounded by thick bands of amorphous to fine fibrillar matrix. This study suggests that chronic pulmonary disease of WHWT is a result of aberrant collagen regulation.
- Published
- 2005
- Full Text
- View/download PDF
15. Pleomorphic rhabdomyosarcoma with pulmonary metastasis in a stranded Steller (northern) sea lion (Eumetopias jubatus).
- Author
-
Zabka TS, Buckles EL, Gulland FM, Haulena M, Naydan DK, and Lowenstine LJ
- Subjects
- Animals, Immunohistochemistry, Liver parasitology, Liver pathology, Lung Neoplasms ultrastructure, Lung Neoplasms veterinary, Microscopy, Electron, Muscle Neoplasms ultrastructure, Muscle Neoplasms veterinary, Rhabdomyosarcoma ultrastructure, Rhabdomyosarcoma veterinary, Lung Neoplasms pathology, Muscle Neoplasms pathology, Parasitic Diseases, Animal pathology, Rhabdomyosarcoma secondary, Sea Lions
- Abstract
A Steller (northern) sea lion (SSL), stranded in northern California in July 2000 had an anaplastic pleomorphic rhabdomyosarcoma in the latissimus dorsi muscle, with pulmonary metastasis. Diagnosis was based on light and electron microscopy and immunohistochemistry. Death was attributed to multiple parasitic and bacterial lesions. The SSL is of special concern because, for unknown reasons, the global population has declined by 50% over the last decade. Published post-mortem data, however, are scarce. This case report highlights several disease conditions that affect this species and is the first report of a malignant neoplasm in a free-ranging SSL.
- Published
- 2004
- Full Text
- View/download PDF
16. Glioblastoma multiforme: clinical findings, magnetic resonance imaging, and pathology in five dogs.
- Author
-
Lipsitz D, Higgins RJ, Kortz GD, Dickinson PJ, Bollen AW, Naydan DK, and LeCouteur RA
- Subjects
- Animals, Biopsy veterinary, Brain Neoplasms metabolism, Brain Neoplasms pathology, Dog Diseases metabolism, Dogs, ErbB Receptors metabolism, Glial Fibrillary Acidic Protein metabolism, Glioblastoma metabolism, Glioblastoma pathology, Immunohistochemistry, Leukocyte Count, Magnetic Resonance Imaging veterinary, Receptors, Vascular Endothelial Growth Factor metabolism, Brain Neoplasms veterinary, Dog Diseases pathology, Glioblastoma veterinary
- Abstract
Although glioblastoma multiforme (GBM), a World Health Organization grade IV astrocytoma, is the most common primary brain tumor in humans, in dogs GBM is relatively rare, accounting for only about 5% of all astrocytomas. This study presents combined clinical, neuroimaging, and neuropathologic findings in five dogs with GBM. The five dogs, aged from 5 to 12 years, were presented with progressive neurologic deficits that subsequent clinical neurologic examination and neuroimaging studies by magnetic resonance imaging (MRI), localized to space occupying lesions in the brain. MRI features of the tumors included consistent peritumoral edema (n = 5), sharp borders (n = 4), ring enhancement (n = 3), heterogenous T2-weighted signal intensity (n = 3), iso- to hypointense T1-weighted images (n = 5), necrosis (n = 5), and cyst formation (n = 2). Two tumors were diagnosed clinically using a computed tomography-guided stereotactic biopsy procedure. At necropsy all the tumors resulted in, on transverse sections, a prominent midline shift and had a variegated appearance due to intratumoral necrosis and hemorrhage. Histologically, they had serpentine necrosis with glial cell pseudopalisading and microvascular proliferation, features which distinguish human GBM from grade III astrocytomas. Immunoreactivity of tumor cells for glial fibrillary acidic protein was strongly positive in all cases, whereas 60% and 40% of the tumors also expressed epidermal growth factor receptor and vascular endothelial growth factor, respectively. These canine GBMs shared many diagnostic neuroimaging, gross, microcopic, and immunoreactivity features similar to those of human GBMs.
- Published
- 2003
- Full Text
- View/download PDF
17. Rapid identification of tissue micro-organisms in skin biopsy specimens from domestic animals using polyclonal BCG antibody.
- Author
-
Bonenberger TE, Ihrke PJ, Naydan DK, and Affolter VK
- Subjects
- Animals, Bacteria isolation & purification, Biopsy veterinary, Camelids, New World, Cats, Dermatomycoses diagnosis, Dermatomycoses microbiology, Dermatomycoses pathology, Dogs, Fungi isolation & purification, Horses, Immunohistochemistry veterinary, Sensitivity and Specificity, Skin Diseases, Bacterial diagnosis, Skin Diseases, Bacterial microbiology, Skin Diseases, Bacterial pathology, Antibodies, Bacterial, Dermatomycoses veterinary, Mycobacterium bovis immunology, Skin microbiology, Skin Diseases, Bacterial veterinary
- Abstract
Immunostaining with polyclonal anti-Mycobacterium bovis (BCG) was evaluated as a single screening method for the histological identification of micro-organisms in skin biopsy specimens from various veterinary species. Confirmed archival cases infected with Mycobacteria, Nocardia, Actinobacillus, Actinomyces, Streptococcus/Staphylococcus, Dermatophilus, spirochetes, Blastomyces, Coccidioides, Cryptococcus, Histoplasma, dermatophytes, Malassezia, Sporothrix, Leishmania, Pythium, phaeohyphomycetes and Prototheca organisms were selected. A total of 70 skin biopsy specimens from the dog, cat, horse, ox and llama were evaluated. The anti-BCG immunostain labelled bacteria and fungi with high sensitivity and minimal background staining but did not label spirochetes and protozoa (Leishmania). Differences were not noted between veterinary species. The results indicate that immunostaining with polyclonal anti-BCG is a suitable screening technique for the rapid identification of most common bacterial and fungal organisms in paraffin-embedded specimens. Also, mycobacterial and nocardial organisms were identified more readily with the anti-BCG immunostain in comparison to the histochemical stains.
- Published
- 2001
- Full Text
- View/download PDF
18. Suprasellar germinoma in three lake whitefish (Coregonus clupeaformis).
- Author
-
Mikaelian I, Lapointe JM, de Lafontaine Y, Harshbarger JC, Côté RJ, Naydan DK, and Martineau D
- Subjects
- Animals, Germinoma pathology, Sella Turcica, Skull Base Neoplasms pathology, Animal Diseases pathology, Brain pathology, Fishes, Germinoma veterinary, Skull Base Neoplasms veterinary
- Abstract
Suprasellar germinomas were identified in three wild-caught lake whitefish (Coregonus clupeaformis) from the St. Lawrence River, Quebec, Canada. Histologically, the three tumors expanded the subarachnoid space of the ventral surface of the brain immediately adjacent to the pituitary gland and, in one case, infiltrated the adjacent neuropil. These tumors were characterized by nests and sheets of round cells with a high mitotic rate, separated by a scant amount of loose fibrovascular stroma. The stroma was infiltrated by a moderate number of small mononuclear cells, including rare CD3-immunoreactive lymphocytes. This is the first report of intracranial germinoma in a fish species.
- Published
- 2000
- Full Text
- View/download PDF
19. Apoptotic and proliferation indexes in canine lymphoma.
- Author
-
Phillips BS, Kass PH, Naydan DK, Winthrop MD, Griffey SM, and Madewell BR
- Subjects
- Animals, Biomarkers, Tumor analysis, Cell Division, Dog Diseases drug therapy, Dogs, Female, Immunohistochemistry, Lymphoma drug therapy, Lymphoma pathology, Male, Mitosis, Prognosis, Treatment Outcome, Apoptosis, Dog Diseases pathology, Ki-67 Antigen analysis, Lymphoma veterinary
- Abstract
Proliferative and apoptotic fractions of tumors were evaluated in 41 dogs with lymphoma for prediction of response to chemotherapy. All dogs had advanced clinical stage tumors, were untreated prior to study, and received identical induction-remission chemotherapy. Tumor cell proliferation was determined in all pretreatment biopsy specimens and in 18 specimens collected at the time of clinical relapse from remission. Quantitative measures included mitotic index and immunoreactivities for proliferating cell nuclear antigen (PCNA) and Ki-67. Apoptotic index was evaluated from 40 dogs pretreatment and from 16 dogs at the time of first relapse. Pretreatment tumor values for Ki-67, PCNA, and apoptosis were compared with posttreatment values. The median first relapse-free interval (RFI) and overall survival (OS) time were 174 days and 445 days, respectively. Of the proliferation markers, only the results of the Ki-67 analysis were predictive for duration of the first RFI but not OS. Pretreatment apoptotic index was also predictive of the duration of first RFI but not OS. No significant predictive value for comparison of the pretreatment and postrelapse values was demonstrated. Ki-67 labeling index and apoptotic indexes were combined to form both a proliferation/apoptotic ratio (PAR) and a sum, or turnover index. Only the PAR was predictive for duration of first RFI on multivariate analysis. Other variables that were evaluated for their influence on treatment outcome included patient age, weight, gender, clinical stage, clinical substage, and tumor immunophenotype. Of these variables, only immunophenotype was found to be of value for predicting duration of first RFI and OS.
- Published
- 2000
- Full Text
- View/download PDF
20. Lymphoma with cutaneous involvement in three domestic rabbits (Oryctolagus cuniculus).
- Author
-
White SD, Campbell T, Logan A, Meredith A, Schultheiss P, Van Winkle T, Moore PF, Naydan DK, and Mallon F
- Abstract
Three domestic rabbits (Oryctolagus cuniculus) with cutaneous lymphoma are described. Two rabbits were young (7 weeks and 1 years) and were euthanized within 1 week of showing clinical signs. Lymphoma was found in the skin and internal organs. The third rabbit was 9.5 years of age, and lived for more than 1 year after diagnosis. No response was seen to either 2 months of alpha-interferon administration or a 2.5-week course of isotretinoin treatment. After 1 year the rabbit died suddenly; the owner refused necropsy. Immunologic stains of the tumour in all three rabbits showed T cells to be the lymphoma cell type., (Blackwell Science Ltd.)
- Published
- 2000
- Full Text
- View/download PDF
21. Clinical and pathologic features of oligodendrogliomas in two cats.
- Author
-
Dickinson PJ, Keel MK, Higgins RJ, Koblik PD, LeCouteur RA, Naydan DK, Bollen AW, and Vernau W
- Subjects
- Animals, Brain pathology, Brain ultrastructure, Brain Neoplasms pathology, Cats, Cerebrospinal Fluid Proteins analysis, Contrast Media chemistry, Erythrocyte Count veterinary, Fatal Outcome, Gadolinium DTPA chemistry, Immunohistochemistry, Leukocyte Count veterinary, Magnetic Resonance Imaging veterinary, Male, Microscopy, Electron, Nervous System Diseases etiology, Oligodendroglioma pathology, Brain Neoplasms veterinary, Cat Diseases pathology, Nervous System Diseases veterinary, Oligodendroglioma veterinary
- Abstract
Two oligodendrogliomas in two domestic cats involved mainly the rostral brain stem, midbrain, fourth ventricle, and cerebellum. Both cats were aged neutered males presenting with clinical neurologic deficits suggestive of a brain stem lesion. Magnetic resonance imaging of both tumors demonstrated lesions with a pattern of heterogeneous contrast enhancement and multifocal lesions in one cat. Routine cerebrospinal fluid analysis was normal in one cat and suggestive of an inflammatory disease in the other. Oligodendroglioma cells were seen in cytospin preparations of cerebrospinal fluid from both cats. In each cat, the tumors occurred intraventricularly in the midbrain and fourth ventricle with aggressive intraparenchymal infiltration. There was extensive growth into the basilar subarachnoid space of the midbrain and brain stem in one cat. One tumor was well differentiated, and the other was an anaplastic subtype. Immunostaining for several myelin- and oligodendroglia-specific antigens was negative with formalin-fixed tumors and with unfixed frozen samples from one cat. In both tumors, component cells of the intratumoral vascular proliferations were positive for human von Willebrand factor VIII antigen or smooth muscle actin. Immunocytochemical reactivity for glial fibrillary acidic protein identified both reactive astrocytes and a subpopulation of minigemistocytes in both tumors. Ultrastructurally, the tumor cells were unremarkable except for their prominent desmosomal junctions and paucity of microtubules.
- Published
- 2000
- Full Text
- View/download PDF
22. Evidence of Pasteurella haemolytica linked immune complex disease in natural and experimental models.
- Author
-
McBride JW, Wozniak EJ, Brewer AW, Naydan DK, and Osburn BI
- Subjects
- Animals, Antigen-Antibody Complex analysis, Blotting, Western veterinary, Cattle, Disease Models, Animal, Electrophoresis, Polyacrylamide Gel veterinary, Enzyme-Linked Immunosorbent Assay veterinary, Immune Complex Diseases immunology, Immune Complex Diseases pathology, Immunoglobulin G analysis, Immunohistochemistry, Male, Mannheimia haemolytica immunology, Mice, Pasteurellosis, Pneumonic pathology, Pulmonary Alveoli immunology, Pulmonary Alveoli pathology, Random Allocation, Retrospective Studies, Immune Complex Diseases veterinary, Mannheimia haemolytica pathogenicity, Pasteurellosis, Pneumonic immunology
- Abstract
The pathogenesis of bovine pneumonic pasteurellosis is not completely understood, and studies have not established that Pasteurella haemolytica A1 (Ph1) virulence is exclusively responsible for the development of acute pulmonary lesions. The purpose of this investigation was to determine if immune complex disease is involved in the pathogenesis of bovine pneumonic pasteurellosis. A retrospective immunohistologic study of lung tissue from natural cases of bovine pneumonic pasteurellosis (44) as performed, and immune complexes were observed in alveloar spaces and walls in 88% of these cases. To study this pathologic mechanism experimentally, groups of mice were immunized with purified Ph1 outer membranes (OMs) or sham immunized on days 0 and 14. Mice were challenged intratracheally on day 24 with either live Ph1 or Ph1 OMs, and pulmonary lesions were assessed 24 h after challenge. Placebo immunized mice developed focal infiltrates of neutrophils and macrophages centered around large caliber bronchi. Mice immunized with Ph1 OMs and challenged with live Ph1 or OMs developed severe bronchointerstitial pneumonia with diffuse neutrophilic infiltration, focal necrosis, hemorrhage and edema, that is histologically similar to bovine pneumonic pasteurellosis. Immunohistology revealed flocculent aggregates of IgG and complement positive material within alveolar spaces and walls from mice challenged with live Ph1, and fine granular deposits of IgG and complement positive material were observed lining the alveolar walls from mice challenged with Ph1 OMs. Immunized mice exhibited high serum IgG antibody titers to Ph1 outer membrane proteins (OMPs). Results of this study suggest that immune complex disease plays a role in the pathogenesis of bovine pneumonic pasteurellosis., (Copyright 1999 Academic Press.)
- Published
- 1999
- Full Text
- View/download PDF
23. Meningoencephalitis due to a Sarcocystis neurona-like protozoan in Pacific harbor seals (Phoca vitulina richardsi).
- Author
-
Lapointe JM, Duignan PJ, Marsh AE, Gulland FM, Barr BC, Naydan DK, King DP, Farman CA, Huntingdon KA, and Lowenstine LJ
- Subjects
- Animals, Antibodies, Protozoan blood, Cerebellar Cortex parasitology, Cerebellar Cortex pathology, Female, Immunohistochemistry, Male, Meningoencephalitis parasitology, Meningoencephalitis pathology, Microscopy, Electron veterinary, Placenta pathology, Pregnancy, Pregnancy Complications, Parasitic parasitology, Pregnancy Complications, Parasitic pathology, Pregnancy Complications, Parasitic veterinary, Sarcocystis immunology, Sarcocystis ultrastructure, Sarcocystosis parasitology, Sarcocystosis pathology, Meningoencephalitis veterinary, Sarcocystis isolation & purification, Sarcocystosis veterinary, Seals, Earless parasitology
- Abstract
Seven Pacific harbor seals with meningoencephalitis associated with Sarcocystis neurona-like protozoa are described. Six of the 7 seals were free-ranging and were found stranded over an 80-km stretch of central California coastline; the other was captive. All had marked to severe nonsuppurative meningoencephalitis, most severe in the cerebellar cortex. Immunohistochemistry for S. neurona antigens was positive on brain tissue in all cases, revealing numerous merozoites as well as developing and mature schizonts, including rosette forms. Electron microscopy performed on 3 animals revealed merozoites and schizonts consistent with Sarcocystis sp., with the absence of rhoptries in merozoites, lack of a parasitophorous vacuole around schizonts, and division by endopolygeny. Serology using western blotting revealed the presence of anti-S. neurona immunoglobulins in the sera of 4 of 5 seals tested. Four animals also had a concurrent mild to moderate nonsuppurative myocarditis; in 1 seal, rare sarcocysts of undetermined species were present within cardiomyocytes.
- Published
- 1998
24. Leukocyte differentiation antigens in canine cutaneous and oral plasmacytomas.
- Author
-
Schrenzel MD, Naydan DK, and Moore PF
- Abstract
Seventeen cutaneous and oral tumours with light microscopic features of plasmacytomas from 16 dogs were studied. Clinically, most neoplasms were benign, although three recurred after excision and three were locally invasive. Tumours most often arose on the pinnae, digits, gingiva, and inguinal regions near areas of chronic inflammation and exhibited variable degrees of plasmacytic differentiation microscopically. Diagnosis of plasmacytoma was confirmed in paraffin-embedded tissues with a panel of leukocyte differentiation antigen markers that included cross-reactive antibodies for Mb-1 (CD79a), CD3, and vimentin and canine-specific antibodies for CD45RA and CD18. Immunoreactivity for Mb-1 and CD45RA, including staining of multinucleate cells and cells with karyomegaly, confirmed a B-cell origin of neoplasms, while staining for CD3 and CD18 revealed an extensive network of infiltrative T-cells and dendritic cells in tumours suggestive of a directed immune response. These findings (i) demonstrate the value of using a panel of antibodies for leukocyte antigens to differentiate plasmacytomas from other cutaneous and oral round cell tumours, and (ii) suggest that immune recognition and responsiveness within tumours may play a role in the behaviour of plasmacytomas in dogs by affecting tumour cell growth and differentiation., (Blackwell Science Ltd.)
- Published
- 1998
- Full Text
- View/download PDF
25. Characterization of the cutaneous inflammatory infiltrate in canine atopic dermatitis.
- Author
-
Olivry T, Naydan DK, and Moore PF
- Subjects
- Allergens immunology, Animals, Antibodies, Monoclonal, Antigen-Presenting Cells immunology, Antigen-Presenting Cells pathology, Antigens immunology, B-Lymphocytes immunology, B-Lymphocytes pathology, Dendritic Cells immunology, Dendritic Cells pathology, Dermatitis, Atopic immunology, Dermatitis, Atopic pathology, Dog Diseases immunology, Dogs, Environmental Exposure, Eosinophils immunology, Eosinophils pathology, Epidermis immunology, Epidermis pathology, Female, Histocompatibility Antigens analysis, Humans, Hyperplasia, Immunoenzyme Techniques, Immunologic Memory immunology, Langerhans Cells immunology, Langerhans Cells pathology, Male, Mast Cells immunology, Mast Cells pathology, Neutrophils immunology, Neutrophils pathology, Receptors, Antigen, T-Cell, gamma-delta analysis, Skin immunology, Skin pathology, Species Specificity, T-Lymphocytes immunology, T-Lymphocytes pathology, T-Lymphocytes, Helper-Inducer immunology, T-Lymphocytes, Helper-Inducer pathology, Dermatitis, Atopic veterinary, Dog Diseases pathology
- Abstract
Sections from lesional atopic, clinically normal atopic, and normal canine skin were investigated by light microscopy and an immunoperoxidase method using monoclonal antibodies specific for canine leukocyte antigens. We confirmed that skin-infiltrating cells of canine atopic dermatitis are constituted of mast cells, dendritic antigen-presenting cells, memory helper T-lymphocytes, low numbers of eosinophils and neutrophils, and rare B-lymphocytes. The presence of epidermal eosinophil microaggregates and clustered Langerhans' cells supports the hypothesis of epidermal allergen contact. The hyperplasia of epidermal T-cells expressing the gamma/delta T-cell receptor appears specific to canine atopic dermatitis compared with its human counterpart. This finding could be explained by an interspecies difference in skin immune systems or, alternatively, by an active participation of these epitheliotropic gamma/delta T-cells in the cutaneous allergic immune response in dogs. The paucity of dermal neutrophils in spontaneous lesions of canine atopic dermatitis is notably different from the neutrophil-rich late-phase reactions provoked by intradermal allergen injections in allergic dogs. This difference in the cellular infiltrate probably results from variations in the immune reaction between single and repeated allergen exposure as well as epidermal versus dermal antigen contact.
- Published
- 1997
- Full Text
- View/download PDF
26. Cytokeratin-filament expression in epithelial and non-epithelial tissues of the common carp (Cyprinus carpio).
- Author
-
Groff JM, Naydan DK, Higgins RJ, and Zinkl JG
- Abstract
Cytokeratin expression in mammals is generally restricted to epithelial cells and has been utilized to differentiate epithelial from nonepithelial tissues in these species. Since cytokeratins have been shown to be highly conserved during vertebrate evolution, the objective of the present study has been to ascertain the expression pattern of cytokeratins in tissues of the common carp (Cyprinus carpio). A panel of 10 anti-human cytokeratin antibodies was evaluated using a streptavidin-biotin-peroxidase complex detection system. Tissues were fixed in 10% neutral-buffered formalin, 100% ethanol or methacarn. Only formalin-fixed tissues were pre-digested with trypsin prior to immunostaining. Formalin-fixed tissues generally resulted in a less intense, more diffuse staining pattern with considerable background compared with ethanol and methacarn and was therefore the least desirable fixative. The diverse staining pattern observed with the various antibodies used in this study was consistent with previous findings in other teleosts. The results confirm that cytokeratin expression in teleosts is fundamentally different from that in mammals and therefore should be used as a method to differentiate epithelial cell types in these species only with discretion.
- Published
- 1997
- Full Text
- View/download PDF
27. Langerhans cell hyperplasia and IgE expression in canine atopic dermatitis.
- Author
-
Olivry T, Moore PF, Affolter VK, and Naydan DK
- Subjects
- Animals, Biopsy, Cell Count, Dermatitis, Atopic immunology, Dermatitis, Atopic pathology, Dogs, Epidermis immunology, Female, Hyperplasia immunology, Hyperplasia pathology, Hyperplasia veterinary, Immunoenzyme Techniques, Langerhans Cells immunology, Male, Reference Values, Skin immunology, Dermatitis, Atopic veterinary, Epidermis pathology, Immunoglobulin E analysis, Langerhans Cells pathology, Skin pathology
- Abstract
Langerhans cells appear to be critical for IgE-mediated allergen capture and presentation in human atopic dermatitis. The present study sought to determine whether epidermal (i.e Langerhans cells) and dermal dendritic cells in the skin of dogs with atopic dermatitis are hyperplastic and expressed surface IgE. Frozen sections of lesional or non-lesional atopic and normal control canine skin were immunostained with CD1a-, CD1c-, and IgE-specific monoclonal antibodies. The enumeration of cells was performed by morphometry in both the epidermis and the dermis. Cell counts were compared with each individual's total serum IgE levels. Higher numbers of epidermal and dermal dendritic cells were present in atopic dogs than in normal control animals. Epidermal Langerhans cell counts were significantly higher in lesional than in non-lesional atopic specimens. IgE+ dendritic cells were observed in lesional atopic epidermis and dermis, and non-lesional atopic dermis, but not in normal control skin specimens. The percentages of IgE+ dendritic cells were correlated with each patient's total serum IgE levels. These results demonstrate dendritic cell hyperplasia and IgE expression in canine atopic dermatitis. Increased epidermal Langerhans cell counts in lesional specimens suggest an epidermal allergen contact in canine atopic dermatitis.
- Published
- 1996
- Full Text
- View/download PDF
28. Antifollicular cell-mediated and humoral immunity in canine alopecia areata.
- Author
-
Olivry T, Moore PF, Naydan DK, Puget BJ, Affolter VK, and Kline AE
- Abstract
Abstract A combination of cellular and humoral immunological assaults directed against follicular matrical cells is suspected to cause alopecia areata (AA) in humans. The specific aims of this study were to determine whether cytotoxic T lymphocytes (CTL) were increased in the blood and skin lesions of canine AA. Additionally, we wished to determine if circulating antifollicular antibodies were present in the dog. Finally, we aimed to investigate whether bulbar inflammation was associated with decreased matrical cell proliferation and keratinocyte apoptosis. Canine AA lesions were infiltrated by intrabulbar CTL, perifollicular helper T cells and dendritic cells. A higher percentage of CTL was present in canine AA peripheral blood compared with that of normal dogs. Tissue-fixed and circulating antifollicular IgG antibodies were detected. Reduced matrical proliferation and increased keratinocyte apoptosis was observed in inflamed hair bulbs. This study demonstrates the existence of antifollicular cell-mediated and humoral immunological responses in canine AA. Résumé- L'association d'agressions immunologiques cellulaires et humorales dirigées contre les cellules matricielles folliculaires est suspectée d'être à l'origine de l'Alopécie Areata chez l'homme. Le but de cette étude était de montrer si le nombre de lymphocytes T cytotoxiques est augmenté dans le sang et dans les lésions cutanées lors d'Alopécie Areata chez le chien. En plus, nous souhaitions montrer la présence d'anticorps circulants antifolliculaire chez le chien. Enfin, nous voulions voir si l'inflammation du bulbe pileux était associée à une diminution de la prolifération des cellules matricielles et une apoptose des kératinocytes. Lors d'Alopécie Areata chez le chien, existe une infiltration lésionnelle par des lymphocytes T cytotoxiques intrabulbaires, des lymphocytes T auxiliaires périfolliculaires et des cellules dentritiques. Le pourcentage de lymphocytes T cytotoxiques était plus important dans le sang périphérique provenant de sujets à Alopécie Areata que dans le sang d'animaux sains. Par ailleurs, des anticorps antifolliculaire (IgG) circulants et fixés dans les tissus sont détectés. Une réduction de la prolifération des cellules matricielles et une augmentation de l'apoptose des kératinocytes sont observées dans les bulbes pileux atteints. Cette étude démontre l'existence de réponses immunologiques cellulaires et humorales contre le follicule pileux dans l'Alopécie Areata du chien. [Olivry, T., Moore, P.F., Naydan, D.K., Puget, B.J., Affolter, V.K., Kline, A.E. Antifollicular cell-mediated and humoral immunity in canine alopecia areata (Immunité contre le follicule pileux dans l'Alopécie Areata du chien). Veterinary Dermatology 1996; 7: 67-79.] Resumen Se cree que la alopecia areata (AA) en la especie humana resulta de la combinación de la acción inmunológica celular y humoral contra células de la matriz folicular. Los objetivos especificos de este estudio fueron determinar si los linfocitos T citotóxicos (CTL) se encontraban aumentados en sangre y en las lesiones cutáneas de animales con AA. Además, descamos investigar la posible presencia de anticuerpos antifoliculares circulantes en el perro. Finalmente, nos propusimos investigar si la inflamación bulbar se encontraba asociada a una disminución en la proliferación de células de la matriz y a la apoptosis de queratinocitos. Las lesiones de la AA canina mostraban infiltración por CTL intrabulbares, células T colaboradoras y células dendríticas. Se encontró un porcentaje mayor de CTL en la sangre periférica de perros con AA respecto a perros normales. Se detectaron anticuerpos IgG antifoliculares circulantes y en tejido fijado. Se observó una disminución en la proliferación matrical y un aumento en queratinocitos apoptóticos en los bulbos foliculares inflamados. Este estudio demuestra la existencia de respuestas inmunitarias antifoliculares de tipo celular y humoral en la AA canina. [Olivry, T., Moore, P.F., Naydan, D.K., Puget, B.J., Affolter, V.K., Kline, A.E. Antifollicular cell-mediated and humoral immunity in canine alopecia areata (Immunidad antifolicular en la alopecia areata canina). Veterinary Dermatology 1996; 7: 67-79.] Zusammenfassung- Eine Kombinaton von zellulären und humoralen immunologischen Angriffen, die gegen die follikulären Matrixzellen gerichtet sind, wird als Ursache bei der Alopecia areata (AA) des Menschen vermutet. Die besondere Absicht dieser Studie war, festzustellen, ob die zytotoxischen T-Lymphozyten (CTL) im Blut und in den Hautveränderungen bei kaniner AA erhöht sind. Zusätzlich wollten wir feststellen, ob beim Hund zirkulierende antifollikuläre Antikörper vorkommen. Schließlich beabsichtigten wir, zu untersuchen, ob die bulbäre Entzündung mit verminderter Matrixzellproliferation und Keratinozytenapoptose einhergeht. Die Veränderungen bei kaniner AA wurden von intrabulbären CTL, perifollikulären T-Helferzellen und dendritischen Zellen infiltriert. Verglichen mit dem peripheren Blut gesunder Hunde kam bei kaniner AA eine höherer Prozentsatz an CTL vor. Es wurden gewebsfixierte und zirkulierende antifollikuläre IgG-Antikörper festgestellt. In entzündeten Haarbulbi wurde eine reduzierte Matrixproliferation und eine erhöhte Keratinozytenapoptose beobachtet. Diese Studie zeigt die Existenz von antifollikulären zellvermittelten und humoralen immunologischen Reaktionen bei kaniner AA. [Olivry, T., Moore, P. F., Naydan, D. K., Puget, B. J., Affolter, V. K., Kline, A. E. Antifollicular immunity in canine alopecia areata (Antifollikuläre Immunität bei der Alopecia areata des Hundes). Veterinary Dermatology 1996; 7: 67-79.].
- Published
- 1996
- Full Text
- View/download PDF
29. Equine papillary ependymoma.
- Author
-
Carrigan MJ, Higgins RJ, Carlson GP, and Naydan DK
- Subjects
- Animals, Brain Chemistry, Brain Neoplasms diagnosis, Brain Neoplasms pathology, Ependymoma diagnosis, Ependymoma pathology, Glial Fibrillary Acidic Protein analysis, Glial Fibrillary Acidic Protein metabolism, Horse Diseases metabolism, Horse Diseases pathology, Horses, Immunohistochemistry, Intermediate Filaments ultrastructure, Male, Vimentin analysis, Vimentin metabolism, Brain Neoplasms veterinary, Ependymoma veterinary, Horse Diseases diagnosis
- Abstract
A 17-year-old Arabian gelding with progressive neurologic signs had a velvety, reddish brain tumor protruding from the ventral midline caudal to the optic chiasma. Histologically, the tumor had a papillary formation with a single layer of elongate cells radially oriented around a central fibrovascular core. Intracytoplasmic globular inclusions were positive for glial fibrillary acidic protein and weakly positive for vimentin. Ultrastructurally, these inclusions were comprised of whorling intermediate filaments. Neoplastic cells also had cytoplasmic interdigitations and numerous zona adherens and often rested on a basal lamina. The tumor was diagnosed as a papillary ependymoma.
- Published
- 1996
- Full Text
- View/download PDF
30. Investigation of epidermotropism in canine mycosis fungoides: expression of intercellular adhesion molecule-1 (ICAM-1) and beta-2 integrins.
- Author
-
Olivry T, Moore PF, Naydan DK, Danilenko DM, and Affolter VK
- Subjects
- Animals, Antibodies, Monoclonal, Biopsy, CD11 Antigens analysis, CD18 Antigens analysis, Dogs, Epitopes analysis, Histocompatibility Antigens Class II analysis, Humans, Immunoenzyme Techniques, Integrins analysis, Intercellular Adhesion Molecule-1 analysis, Lymphocyte Function-Associated Antigen-1 analysis, Lymphocytes pathology, Mycosis Fungoides immunology, Mycosis Fungoides pathology, Skin pathology, Dog Diseases, Integrins biosynthesis, Intercellular Adhesion Molecule-1 biosynthesis, Lymphocytes immunology, Mycosis Fungoides veterinary, Skin immunology
- Abstract
In human mycosis fungoides (MF), interactions between LFA-1 (CD11a/CD18) and ICAM-1 (CD54) are involved in lymphocyte adhesion to keratinocytes. The purpose of this study was to evaluate the expression of ICAM-1, beta-2 integrins and class II major histocompatibility complex molecules (MHC II) on keratinocytes and infiltrating lymphocytes in canine MF. Sections of frozen skin biopsy specimens from normal dogs (n = 3) and dogs with MF (n = 17) were evaluated by immunohistochemistry for expression of ICAM-1, beta-2 integrins, and class II MHC molecules. Our results demonstrated that in canine MF, ICAM-1 was expressed variably on epidermal and follicular keratinocytes. The extent of keratinocyte ICAM-1 expression did not correlate with the degree of lymphocyte epithelial infiltration, nor with lymphocyte LFA-1 expression. This was especially evident in cases of Pagetoid reticulosis-like disease in which prominent lymphocyte epidermotropism was not accompanied by keratinocyte ICAM-1 expression. Keratinocyte class II MHC molecule expression did not correlate with keratinocyte ICAM-1 expression. In conclusion, in canine MF, the lack of statistically significant correlations between epithelial lymphocyte infiltration and keratinocyte ICAM-1 expression, and between keratinocyte ICAM-1 and lymphocyte LFA-1 staining, suggests that the LFA-1/ICAM-1 pathway is not the major adhesion mechanism between lymphocytes and keratinocytes. It is suspected that different ligands of the LFA-1 integrin (e.g. ICAM-2) or other adhesion molecules (e.g. CD2/LFA-3, VLA-1) might be involved in the epitheliotropism phenomenon in canine MF. These hypothesis cannot be evaluated in the dog at this time owing to the lack of specific monoclonal antibodies.
- Published
- 1995
- Full Text
- View/download PDF
31. Immunohistochemical reactivity of basal and luminal epithelium-specific cytokeratin antibodies within normal and neoplastic canine mammary glands.
- Author
-
Griffey SM, Madewell BR, Dairkee SH, Hunt JE, Naydan DK, and Higgins RJ
- Subjects
- Actins analysis, Adenocarcinoma chemistry, Adenocarcinoma pathology, Adenoma chemistry, Adenoma pathology, Animals, Dogs, Female, Hyperplasia pathology, Hyperplasia veterinary, Immunohistochemistry, Keratins analysis, Mammary Glands, Animal chemistry, Mammary Neoplasms, Animal chemistry, Vimentin analysis, Adenocarcinoma veterinary, Adenoma veterinary, Dog Diseases pathology, Mammary Glands, Animal pathology, Mammary Neoplasms, Animal pathology
- Abstract
Human basal epithelium (myoepithelium)-specific (312C8-1) and luminal epithelium-specific (13H5) cytokeratin antibodies were applied to frozen sections of normal canine mammary tissues (seven), benign adenomas and hyperplasias (five), mixed tumors (12), and adenocarcinomas (18) to determine if epithelial subsets could be discriminated by the use of an avidin biotin peroxidase complex immunohistochemical procedure. The 312C8-1 and 13H5 antibodies were consistently reactive with basal and luminal epithelium, respectively, in the normal mammary gland (7/7) and in benign adenomas and hyperplasias (5/5). Mixed mammary tumors had similar basal and luminal epithelial reactivity and also had proliferating spindle-shaped stromal cells that were reactive with 312C8-1 (10/12) and 13H5 (4/12). The adenocarcinomas were subclassified into basal, luminal, and basal/luminal on the basis of 312C8-1 reactivity (4/18), 13H5 reactivity (2/18), and dual reactivity with mutually exclusive anatomic distribution (11/18), respectively. Those tumors with dual immunoreactivity were indicative of noninvasive carcinomas. Dogs with neoplasms that were reactive with 312C8-1 and nonreactive with 13H5 had local recurrence or distant metastasis within 2 weeks to 6 months after diagnosis. Other antibodies used for comparison were pan cytokeratin AE1/AE3, actin HHF35, and vimentin. 312C8-1 and 13H5 antibodies are specific for canine mammary basal and luminal epithelium, respectively, and by employing these antibodies, the origin and differentiation of canine mammary neoplasms can be determined more accurately than on the basis of hematoxylin and eosin-stained tissue alone.
- Published
- 1993
- Full Text
- View/download PDF
32. Evaluation of commercially available antibodies to cytokeratin intermediate filaments and laminin in normal cat pinna.
- Author
-
Peaston AE, Higgins RJ, Naydan DK, and Sokol KA
- Subjects
- Animals, Antibodies, Monoclonal immunology, Apocrine Glands chemistry, Basement Membrane chemistry, Ear, External ultrastructure, Epidermis chemistry, Epithelium chemistry, Evaluation Studies as Topic, Hair chemistry, Immunohistochemistry, Intermediate Filaments chemistry, Intermediate Filaments immunology, Keratins immunology, Laminin immunology, Sebaceous Glands chemistry, Skin chemistry, Skin ultrastructure, Autoantibodies immunology, Cats anatomy & histology, Ear, External chemistry, Keratins analysis, Laminin analysis
- Abstract
The pattern of distribution of cytokeratin (CK) intermediate filaments can be used to characterize subsets of epithelial tissues. The purpose of the study was to examine the CK expression of feline pinna skin. Six normal feline pinnae were routinely processed in formalin. An immunohistochemical method was used to stain the pinnae with 8 commercially available anti-human CK antibodies (Abs) (PKK1, CAM 5.2, UCD 10/11, 35BH11, 34BE12, AE1/AE3, MAK 6, A575) and an anti-human laminin Ab. All the CK Abs selectively localized to epithelium except 35BH11, which did not react with any part of the pinna. Some epithelial subsets were identified by their unique staining pattern with CK Abs. Basal cells but not suprabasal cells of the epidermis stained with PKK1; basal but not lumenal cells of apocrine glands stained with 34BE12. Apocrine glands stained with all CK Abs except 35BH11. All epithelial structures were stained with A575. Basal lamina of epithelial and mesenchymal tissues was clearly identified by the anti-laminin Ab. The results indicate that in cat pinna some commercially available anti-human CK Abs selectively stain subsets of epithelium and adnexa. PKK1, 34BE12, and A575 were the CK Abs with the most consistent staining patterns, the other Abs stained more variably from pinna to pinna. The pattern of epithelial and adnexal staining was similar but not identical to that reported for humans.
- Published
- 1992
- Full Text
- View/download PDF
33. An atypical neuroendocrine tumor in the lung of a beagle dog.
- Author
-
Harkema JR, Jones SE, Naydan DK, and Wilson DW
- Subjects
- Animals, Carcinoma, Bronchogenic chemistry, Carcinoma, Bronchogenic pathology, Carcinoma, Bronchogenic ultrastructure, Dogs, Female, Immunohistochemistry, Lung Neoplasms chemistry, Lung Neoplasms pathology, Lung Neoplasms ultrastructure, Microscopy, Electron, Phosphopyruvate Hydratase analysis, Carcinoma, Bronchogenic veterinary, Dog Diseases pathology, Lung Neoplasms veterinary
- Published
- 1992
- Full Text
- View/download PDF
34. A comparison of six monoclonal antibodies for detection of cytokeratins in normal and neoplastic canine tissues.
- Author
-
Cardona A, Madewell BR, Naydan DK, and Lund JK
- Subjects
- Animals, Dogs, Epithelium chemistry, Immunohistochemistry, Keratins immunology, Neoplasms chemistry, Neoplasms diagnosis, Antibodies, Monoclonal, Dog Diseases diagnosis, Keratins analysis, Neoplasms veterinary
- Abstract
Twenty normal canine tissue specimens, both fetal and adult; 19 epithelial neoplasms; and 18 nonepithelial neoplasms were examined using 6 commercially available monoclonal antibodies differing in their recognition of various molecular weight cytokeratins in human tissues. Fresh tissue samples were fixed in 100% ethanol and paraffin embedded prior to sectioning. The intermediate filament proteins were identified by an avidin-biotin-immunoperoxidase method. Primary antisera used included AE1/AE3, CAM-5.2, 35BH11, 34BE12, PKK1, MAK-6 cytokeratins, and vimentin. Monoclonal antibodies detected cytokeratins in a wide variety of canine epithelial tissues and neoplasms. Normal mesenchymal tissues and neoplasms, and stromal elements of epithelial tissues, showed no reactivity with anti-cytokeratins, but reacted positively with vimentin. Although PKK1, CAM-5.2, and MAK-6 were the most consistently reactive anti-cytokeratins, the full panel of monoclonals was required to detect cytokeratins in all of the epithelia evaluated.
- Published
- 1989
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.