14 results on '"Narmen Azazmeh"'
Search Results
2. Stimulation of epidermal hyperplasia and tumorigenesis by resident p16INK4a-expressing cells
- Author
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Narmen Azazmeh and Ittai Ben-Porath
- Subjects
p16ink4a ,cdkn2a ,senescence ,aging ,epidermis ,wnt ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
p16INK4a (CDKN2A) is a central tumor-suppressor and activator of senescence. We recently found that prolonged expression of p16INK4a in epidermal cells induces hyperplasia and dysplasia through Wnt-mediated stimulation of neighboring keratinocytes. The study suggests a pro-tumorigenic function of p16INK4a in early epidermal lesions, which could potentially be targeted by senolytic therapy.
- Published
- 2020
- Full Text
- View/download PDF
3. Suppression of adipose lipolysis by long-chain fatty acid analogs
- Author
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Bella Kalderon, Narmen Azazmeh, Nili Azulay, Noam Vissler, Michael Valitsky, and Jacob Bar-Tana
- Subjects
cAMP ,AMPK ,Raf1 ,diabetes ,Biochemistry ,QD415-436 - Abstract
Agonist-induced lipolysis of adipose fat is robustly inhibited by insulin or by feedback inhibition by the long-chain fatty acids (LCFA) produced during lipolysis. However, the mode of action of LCFA in suppressing adipose lipolysis is not clear. β,β’-Tetramethyl hexadecanedioic acid (Mββ/ EDICA16) is a synthetic LCFA that is neither esterified into lipids nor β-oxidized, and therefore, it was exploited for suppressing agonist-induced lipolysis in analogy to natural LCFA. Mββ is shown here to suppress isoproterenol-induced lipolysis in the rat in vivo as well as in 3T3-L1 adipocytes. Inhibition of isoproterenol-induced lipolysis is due to decrease in isoproterenol-induced cAMP with concomitant inhibition of the phosphorylation of hormone-sensitive lipase and perilipin by protein kinase A. Suppression of cellular cAMP levels is accounted for by inhibition of the adenylate cyclase due to suppression of Raf1 expression by Mββ-activated AMPK. Suppression of Raf1 is further complemented by induction of components of the unfolded-protein-response by Mββ. Our findings imply genuine inhibition of agonist-induced adipose lipolysis by LCFA, independent of their β-oxidation or reesterification. Mββ suppression of agonist-induced lipolysis and cellular cAMP levels independent of the insulin transduction pathway may indicate that synthetic LCFA could serve as insulin mimetics in the lipolysis context under conditions of insulin resistance.
- Published
- 2012
- Full Text
- View/download PDF
4. Supplementary Methods and Figure Legend from Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis
- Author
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Ittai Ben-Porath, Alexander Maly, Alia Hassan, Yan Stein, Aharon Helman, Narmen Azazmeh, and Ronit Tokarsky-Amiel
- Abstract
PDF file - 90K
- Published
- 2023
5. Data from Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis
- Author
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Ittai Ben-Porath, Alexander Maly, Alia Hassan, Yan Stein, Aharon Helman, Narmen Azazmeh, and Ronit Tokarsky-Amiel
- Abstract
Cellular senescence, a state of cell-cycle arrest accompanied by dramatic morphologic and metabolic changes, is a central means by which cells respond to physiologic stress and oncogene activity. Senescence is thought to play important roles in aging and in tumor suppression, yet the dynamics by which senescent cells are formed, their effects on tissue function and their eventual fate are poorly understood. To study cellular senescence within an adult tissue, we developed transgenic mice inducibly expressing p14ARF (human ortholog of murine p19ARF), a central activator of senescence. Induction of p14ARF in the epidermis rapidly led to widespread apoptosis and cell-cycle arrest, a stage that was transient, and was followed by p53-dependent cellular senescence. The endogenous Cdkn2a products p19ARF and p16Ink4a were activated by the transgenic p14ARF through p53, revealing a senescence-promoting feed-forward loop. Commitment of cells to senescence required continued p14ARF expression, indicating that entry into this state depends on a persistent signal. However, once formed, senescent cells were retained in the epidermis, often for weeks after transgene silencing, indicating an absence of an efficient rapidly acting mechanism for their removal. Stem cells in the hair follicle bulge were largely protected from apoptosis upon p14ARF induction, but irreversibly lost their ability to proliferate and initiate follicle growth. Interestingly, induction of epidermal hyperplasia prevented the appearance of senescent cells upon p14ARF induction. Our findings provide basic insights into the dynamics of cellular senescence, a central tumor- suppressive mechanism, and reveal the potential for prolonged retention of senescent cells within tissues. Cancer Res; 73(9); 2829–39. ©2013 AACR.
- Published
- 2023
6. Supplementary Figures 1 - 6 from Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis
- Author
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Ittai Ben-Porath, Alexander Maly, Alia Hassan, Yan Stein, Aharon Helman, Narmen Azazmeh, and Ronit Tokarsky-Amiel
- Abstract
PDF file - 118K, Supplementary Figure S1. Species specificity of p14ARF and p19ARF antibodies. Supplementary Figure S2. Increase in SAβGal-positive cell numbers in control mice during early aging. Supplementary Figure S3. Rb and p130 dephosphorylation upon p14ARF induction. Supplementary Figure S4. Effects of p14ARF induction on skin histology. Supplementary Figure S5. p14ARF induces hair-follicle stem cell dysfunction through p53. Supplementary Figure S6. Senescence in TPA-treated mice.
- Published
- 2023
7. Chronic expression of p16INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation
- Author
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Shmuel Ruppo, Eli Pikarsky, Agnieszka K. Witkiewicz, Alexander Maly, Sophia V. Rizou, Yuval Nevo, Karen Meir, Benjamin Assouline, Eitan Winter, Jonathan Cohen, Ittai Ben-Porath, Chen Luxenburg, Narmen Azazmeh, and Vassilis G. Gorgoulis
- Subjects
0301 basic medicine ,Transgene ,Science ,Cell ,General Physics and Astronomy ,Tumor initiation ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Paracrine signalling ,0302 clinical medicine ,CDKN2A ,medicine ,lcsh:Science ,Multidisciplinary ,integumentary system ,Epidermis (botany) ,Chemistry ,Wnt signaling pathway ,General Chemistry ,Hyperplasia ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,lcsh:Q - Abstract
p16INK4a (CDKN2A) is a central tumor suppressor, which induces cell-cycle arrest and senescence. Cells expressing p16INK4a accumulate in aging tissues and appear in premalignant lesions, yet their physiologic effects are poorly understood. We found that prolonged expression of transgenic p16INK4a in the mouse epidermis induces hyperplasia and dysplasia, involving high proliferation rates of keratinocytes not expressing the transgene. Continuous p16INK4a expression increases the number of epidermal papillomas formed after carcinogen treatment. Wnt-pathway ligands and targets are activated upon prolonged p16INK4a expression, and Wnt inhibition suppresses p16INK4a-induced hyperplasia. Senolytic treatment reduces p16INK4a-expressing cell numbers, and inhibits Wnt activation and hyperplasia. In human actinic keratosis, a precursor of squamous cell carcinoma, p16INK4a-expressing cells are found adjacent to dividing cells, consistent with paracrine interaction. These findings reveal that chronic p16INK4a expression is sufficient to induce hyperplasia through Wnt-mediated paracrine stimulation, and suggest that this tumor suppressor can promote early premalignant epidermal lesion formation.
- Published
- 2020
8. MODL-38. DEVELOPMENTAL EFFECTS OF MYBL1 ACTIVATION ON MURINE BRAIN AND GLIAL DEVELOPMENT
- Author
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Narmen Azazmeh, Cecile Rouleau, Kate Schoolcraft, Etai Jacob, Seth Malinowski, John P Busanovich, Lori Ramkissoon, Yun Jee Kang, Shakti Ramkissoon, Kristine Pelton, Alice Meng, Victor Jones, Rodderick Bronson, Federica Piccioni, Claudia Kleinman, Pratiti Bandopadhayay, Keith Ligon, and Rameen Beroukhim
- Subjects
Cancer Research ,Oncology ,Neurology (clinical) - Abstract
Low-grade gliomas (LGG) represent 30% of pediatric brain tumors. Their cellular origins are unknown but are presumed to arise from subtle alterations of progenitor cell cycle regulation during brain development. Rearrangements activating MYB and MYBL1 have been identified as drivers of LGG in angiocentric glioma and diffuse astrocytomas, respectively, but the roles of these genes in the normal brain and the development of LGG are poorly understood. We first performed a developmental analysis of human and mouse Mybl1 expression from bulk and single-cell RNA-sequencing and identified exclusive expression of MYBL1 in neural stem and progenitor cells in the ganglionic eminence. We also found that MYBL1high cell transcriptomes are enriched in genes functionally involved in centromere and mitotic processes, strongly suggesting an association between MYBL1 expression and cellular proliferation states. We next hypothesized that C-terminal truncation may drive tumorigenesis through a direct increase in MYBL1 expression and cell proliferation. We developed a novel Cre-dependent knock-in mouse-model for human truncated MYBL1 expression and tested effects in oligodendroglial(Olig2-cre), astrocytic hGFAP-cre), and somatic(Ubiquitin-cre) cell types. In Ubq-cre:R26-MYBL1-tr mice there was expression and dysplasia in the salivary gland but no significant effects on brain development. In Olig2-Cre+/tg:R26-MYBL1-tr+/fl mice we observed higher susceptibility to motor seizures, early postnatal death without gross or microscopic abnormalities of brain morphology. In contrast, expression in stem cells and astrocytes of hGFAP-Cre+/tg:R26-MYBL1-tr+/flmice drove dramatic abnormalities in brain development and altered proliferation of progenitor and stem cells, but not glioma formation. Single-cell RNA sequencing of MYBL1-tr cells from mNSCs and brains of mice were also used to determine patterns of altered expression driven by MYBL1-tr. These results indicate that aberrant MYBL1 activation affects neural stem/progenitor cell and brain development through altered cell proliferation. Future targeting of the pathways identified may be therapeutically beneficial for patients with LGG driven by MYB-family oncogenes.
- Published
- 2022
9. Senolytic elimination of Cox2-expressing senescent cells inhibits the growth of premalignant pancreatic lesions
- Author
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Areej Khatib, Lior Roitman, Sharona Elgavish, Narmen Azazmeh, Anna Hochner-Ger, Eli Pikarsky, Gideon Zamir, Rachel Kalifa, Benjamin Assouline, Jonathan Demma, Yonatan Khalatnik, Yehuda Schlesinger, Yuval Dor, Karen Meir, Hadar Benyamini, Valery Krizhanovsky, Oren Parnas, Ittai Ben-Porath, Shaul Horwitz, Ashraf Imam, Karine Atlan, Yossi Ovadya, Dror Kolodkin-Gal, and Eitan Winter
- Subjects
pancreatic tumours ,0301 basic medicine ,Pancreatic ductal adenocarcinoma ,endocrine system diseases ,cell cycle control ,Pancreatic Intraepithelial Neoplasia ,Cellular senescence ,Adenocarcinoma ,Biology ,Disease course ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,Paracrine signalling ,0302 clinical medicine ,Senotherapeutics ,cell biology ,Animals ,Senolytic ,Pancreas ,Cellular Senescence ,Gastroenterology ,3. Good health ,Pancreatic Neoplasms ,Disease Models, Animal ,030104 developmental biology ,Tumour development ,Cyclooxygenase 2 ,030220 oncology & carcinogenesis ,Cancer research ,Precancerous Conditions - Abstract
ObjectiveCellular senescence limits tumourigenesis by blocking the proliferation of premalignant cells. Additionally, however, senescent cells can exert paracrine effects influencing tumour growth. Senescent cells are present in premalignant pancreatic intraepithelial neoplasia (PanIN) lesions, yet their effects on the disease are poorly characterised. It is currently unknown whether senolytic drugs, aimed at eliminating senescent cells from lesions, could be beneficial in blocking tumour development.DesignTo uncover the functions of senescent cells and their potential contribution to early pancreatic tumourigenesis, we isolated and characterised senescent cells from PanINs formed in a Kras-driven mouse model, and tested the consequences of their targeted elimination through senolytic treatment.ResultsWe found that senescent PanIN cells exert a tumour-promoting effect through expression of a proinflammatory signature that includes high Cox2 levels. Senolytic treatment with the Bcl2-family inhibitor ABT-737 eliminated Cox2-expressing senescent cells, and an intermittent short-duration treatment course dramatically reduced PanIN development and progression to pancreatic ductal adenocarcinoma.ConclusionsThese findings reveal that senescent PanIN cells support tumour growth and progression, and provide a first indication that elimination of senescent cells may be effective as preventive therapy for the progression of precancerous lesions.
- Published
- 2021
- Full Text
- View/download PDF
10. Chronic expression of p16
- Author
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Narmen, Azazmeh, Benjamin, Assouline, Eitan, Winter, Shmuel, Ruppo, Yuval, Nevo, Alexander, Maly, Karen, Meir, Agnieszka K, Witkiewicz, Jonathan, Cohen, Sophia V, Rizou, Eli, Pikarsky, Chen, Luxenburg, Vassilis G, Gorgoulis, and Ittai, Ben-Porath
- Subjects
Keratinocytes ,Hyperplasia ,Mice, 129 Strain ,Papilloma ,Mice, Transgenic ,Keratosis ,Article ,Mice, Inbred C57BL ,Cell Transformation, Neoplastic ,Animals ,Humans ,Skin cancer ,Epidermis ,Cancer models ,Tumour-suppressor proteins ,neoplasms ,Wnt Signaling Pathway ,Cells, Cultured ,Cyclin-Dependent Kinase Inhibitor p16 ,Cell Proliferation - Abstract
p16INK4a (CDKN2A) is a central tumor suppressor, which induces cell-cycle arrest and senescence. Cells expressing p16INK4a accumulate in aging tissues and appear in premalignant lesions, yet their physiologic effects are poorly understood. We found that prolonged expression of transgenic p16INK4a in the mouse epidermis induces hyperplasia and dysplasia, involving high proliferation rates of keratinocytes not expressing the transgene. Continuous p16INK4a expression increases the number of epidermal papillomas formed after carcinogen treatment. Wnt-pathway ligands and targets are activated upon prolonged p16INK4a expression, and Wnt inhibition suppresses p16INK4a-induced hyperplasia. Senolytic treatment reduces p16INK4a-expressing cell numbers, and inhibits Wnt activation and hyperplasia. In human actinic keratosis, a precursor of squamous cell carcinoma, p16INK4a-expressing cells are found adjacent to dividing cells, consistent with paracrine interaction. These findings reveal that chronic p16INK4a expression is sufficient to induce hyperplasia through Wnt-mediated paracrine stimulation, and suggest that this tumor suppressor can promote early premalignant epidermal lesion formation., It is unclear how resident p16-expressing senescent cells affect the propensity of tissues to develop cancer. Here, the authors show that chronic p16 expression in the mouse epidermis causes hyperplasia and dysplasia through Wnt-mediated paracrine stimulation of proliferating keratinocytes, and can contribute to tumour formation.
- Published
- 2018
11. p16Ink4a-induced senescence of pancreatic beta cells enhances insulin secretion
- Author
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Amit Zamir, Ittai Ben-Porath, Sharona Tornovsky-Babeay, Yael Gabai, Reba Condiotti, Shira Anzi, Yaakov Fixler, A. M. James Shapiro, Agnes Klochendler, Elad Horwitz, Aharon Helman, Narmen Azazmeh, Alvin C. Powers, Chunhua Dai, Mark A. Magnuson, Roy Z. Granit, Benjamin Glaser, Avital Swisa, Dorin Shreibman, Yuval Dor, and Yuval Nevo
- Subjects
0301 basic medicine ,Senescence ,Aging ,medicine.medical_specialty ,medicine.medical_treatment ,Glucose uptake ,Peroxisome proliferator-activated receptor ,Mice, Transgenic ,Article ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,Insulin-Secreting Cells ,Internal medicine ,Insulin Secretion ,medicine ,Animals ,Humans ,Insulin ,Glucose homeostasis ,neoplasms ,Cellular Senescence ,Cyclin-Dependent Kinase Inhibitor p16 ,Cell Proliferation ,chemistry.chemical_classification ,TOR Serine-Threonine Kinases ,General Medicine ,Fibroblasts ,Insulin oscillation ,PPAR gamma ,Glucose ,030104 developmental biology ,Endocrinology ,Gene Expression Regulation ,chemistry ,Beta cell ,Cell aging - Abstract
Cellular senescence is thought to contribute to age-associated deterioration of tissue physiology. The senescence effector p16(Ink4a) is expressed in pancreatic beta cells during aging and limits their proliferative potential; however, its effects on beta cell function are poorly characterized. We found that beta cell-specific activation of p16(Ink4a) in transgenic mice enhances glucose-stimulated insulin secretion (GSIS). In mice with diabetes, this leads to improved glucose homeostasis, providing an unexpected functional benefit. Expression of p16(Ink4a) in beta cells induces hallmarks of senescence--including cell enlargement, and greater glucose uptake and mitochondrial activity--which promote increased insulin secretion. GSIS increases during the normal aging of mice and is driven by elevated p16(Ink4a) activity. We found that islets from human adults contain p16(Ink4a)-expressing senescent beta cells and that senescence induced by p16(Ink4a) in a human beta cell line increases insulin secretion in a manner dependent, in part, on the activity of the mechanistic target of rapamycin (mTOR) and the peroxisome proliferator-activated receptor (PPAR)-γ proteins. Our findings reveal a novel role for p16(Ink4a) and cellular senescence in promoting insulin secretion by beta cells and in regulating normal functional tissue maturation with age.
- Published
- 2016
12. Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis
- Author
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Yan Stein, Ittai Ben-Porath, Alexander Maly, Aharon Helman, Ronit Tokarsky-Amiel, Narmen Azazmeh, and Alia Hassan
- Subjects
Senescence ,Genetically modified mouse ,Cancer Research ,Transgene ,Apoptosis ,Mice, Transgenic ,Endogeny ,Biology ,Models, Biological ,Mice ,Tumor Suppressor Protein p14ARF ,Animals ,Humans ,Gene Silencing ,Transgenes ,Cellular Senescence ,Epidermis (botany) ,Cell Cycle ,Cell cycle ,beta-Galactosidase ,Cell biology ,Oncology ,RNA ,Epidermis ,Tumor Suppressor Protein p53 ,Stem cell ,Hair Follicle - Abstract
Cellular senescence, a state of cell-cycle arrest accompanied by dramatic morphologic and metabolic changes, is a central means by which cells respond to physiologic stress and oncogene activity. Senescence is thought to play important roles in aging and in tumor suppression, yet the dynamics by which senescent cells are formed, their effects on tissue function and their eventual fate are poorly understood. To study cellular senescence within an adult tissue, we developed transgenic mice inducibly expressing p14ARF (human ortholog of murine p19ARF), a central activator of senescence. Induction of p14ARF in the epidermis rapidly led to widespread apoptosis and cell-cycle arrest, a stage that was transient, and was followed by p53-dependent cellular senescence. The endogenous Cdkn2a products p19ARF and p16Ink4a were activated by the transgenic p14ARF through p53, revealing a senescence-promoting feed-forward loop. Commitment of cells to senescence required continued p14ARF expression, indicating that entry into this state depends on a persistent signal. However, once formed, senescent cells were retained in the epidermis, often for weeks after transgene silencing, indicating an absence of an efficient rapidly acting mechanism for their removal. Stem cells in the hair follicle bulge were largely protected from apoptosis upon p14ARF induction, but irreversibly lost their ability to proliferate and initiate follicle growth. Interestingly, induction of epidermal hyperplasia prevented the appearance of senescent cells upon p14ARF induction. Our findings provide basic insights into the dynamics of cellular senescence, a central tumor- suppressive mechanism, and reveal the potential for prolonged retention of senescent cells within tissues. Cancer Res; 73(9); 2829–39. ©2013 AACR.
- Published
- 2013
13. Suppression of adipose lipolysis by long-chain fatty acid analogs
- Author
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Noam Vissler, Narmen Azazmeh, Jacob Bar-Tana, Bella Kalderon, Nili Azulay, and Michael Valitsky
- Subjects
AMPK ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Lipolysis ,Adipose tissue ,QD415-436 ,AMP-Activated Protein Kinases ,Biochemistry ,Mice ,Endocrinology ,Insulin resistance ,AMP-activated protein kinase ,cAMP ,Internal medicine ,3T3-L1 Cells ,medicine ,Cyclic AMP ,Animals ,Raf1 ,Rats, Wistar ,Protein kinase A ,Research Articles ,diabetes ,biology ,Chemistry ,Insulin ,Fatty Acids ,Isoproterenol ,Cell Biology ,medicine.disease ,Aminoimidazole Carboxamide ,MAP Kinase Kinase Kinases ,Cyclic AMP-Dependent Protein Kinases ,Rats ,Proto-Oncogene Proteins c-raf ,Adipose Tissue ,biology.protein ,Perilipin ,Unfolded Protein Response ,lipids (amino acids, peptides, and proteins) ,Ribonucleosides ,Adenylyl Cyclases ,Signal Transduction - Abstract
Agonist-induced lipolysis of adipose fat is robustly inhibited by insulin or by feedback inhibition by the long-chain fatty acids (LCFA) produced during lipolysis. However, the mode of action of LCFA in suppressing adipose lipolysis is not clear. β,β’-Tetramethyl hexadecanedioic acid (Mββ/ EDICA16) is a synthetic LCFA that is neither esterified into lipids nor β-oxidized, and therefore, it was exploited for suppressing agonist-induced lipolysis in analogy to natural LCFA. Mββ is shown here to suppress isoproterenol-induced lipolysis in the rat in vivo as well as in 3T3-L1 adipocytes. Inhibition of isoproterenol-induced lipolysis is due to decrease in isoproterenol-induced cAMP with concomitant inhibition of the phosphorylation of hormone-sensitive lipase and perilipin by protein kinase A. Suppression of cellular cAMP levels is accounted for by inhibition of the adenylate cyclase due to suppression of Raf1 expression by Mββ-activated AMPK. Suppression of Raf1 is further complemented by induction of components of the unfolded-protein-response by Mββ. Our findings imply genuine inhibition of agonist-induced adipose lipolysis by LCFA, independent of their β-oxidation or reesterification. Mββ suppression of agonist-induced lipolysis and cellular cAMP levels independent of the insulin transduction pathway may indicate that synthetic LCFA could serve as insulin mimetics in the lipolysis context under conditions of insulin resistance.
- Published
- 2012
14. Gating of the mitochondrial permeability transition pore by thyroid hormone
- Author
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Einav Yehuda-Shnaidman, Bella Kalderon, Narmen Azazmeh, and Jacob Bar-Tana
- Subjects
Male ,Mitochondria, Liver ,Gating ,Oxidative phosphorylation ,In Vitro Techniques ,Biochemistry ,Mitochondrial Membrane Transport Proteins ,Mitochondrial membrane transport protein ,Jurkat Cells ,Bcl-2-associated X protein ,Genetics ,Animals ,Humans ,Inositol 1,4,5-Trisphosphate Receptors ,Calcium Signaling ,Molecular Biology ,Calcium signaling ,DNA Primers ,bcl-2-Associated X Protein ,Membrane Potential, Mitochondrial ,biology ,Base Sequence ,Chemistry ,Mitochondrial Permeability Transition Pore ,Endoplasmic reticulum ,Calcineurin ,Inositol trisphosphate receptor ,Cell biology ,Rats ,Mitochondrial permeability transition pore ,Proto-Oncogene Proteins c-bcl-2 ,biology.protein ,Triiodothyronine ,Energy Metabolism ,Ion Channel Gating ,Biotechnology ,Signal Transduction - Abstract
The calorigenic-thermogenic activity of thyroid hormone (T3) has long been ascribed to uncoupling of mitochondrial oxidative phosphorylation. However, the mode of action of T3 in promoting mitochondrial proton leak is still unresolved. Mitochondrial uncoupling by T3 is reported here to be transduced in vivo in rats and in cultured Jurkat cells by gating of the mitochondrial permeability transition pore (PTP). T3-induced PTP gating is shown here to be abrogated in inositol 1,4,5-trisphosphate (IP(3)) receptor 1 (IP(3)R1)(-/-) cells, indicating that the endoplasmic reticulum IP(3)R1 may serve as upstream target for the mitochondrial activity of T3. IP(3)R1 gating by T3 is due to its increased expression and truncation into channel-only peptides, resulting in IP(3)-independent Ca(2+) efflux. Increased cytosolic Ca(2+) results in activation of protein phosphatase 2B, dephosphorylation and depletion of mitochondrial Bcl2 (S70), and increase in mitochondrial free Bax leading to low-conductance PTP gating. The T3 transduction pathway integrates genomic and nongenomic activities of T3 in regulating mitochondrial energetics and may offer novel targets for thyromimetics designed to modulate energy expenditure.
- Published
- 2009
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