1. Stability and antioxidant activity of gossypol derivative immobilized on N-polyvinylpyrrolidone
- Author
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Elmira Tokhtaeva, Khairulla Ziyaev, Maksim Ionov, N. Gordiyenko, Maria Zamaraeva, Kuralbay Rezhepov, Izabela Zukowska, Olga A. Mareninova, and N. I. Baram
- Subjects
Male ,Antioxidant ,DPPH ,Thiobarbituric acid ,medicine.medical_treatment ,macromolecular substances ,medicine.disease_cause ,Biochemistry ,Lipid peroxidation ,chemistry.chemical_compound ,Drug Stability ,Picrates ,Structural Biology ,medicine ,Animals ,Antipain ,Benzothiazoles ,Rats, Wistar ,Carbon Tetrachloride ,Molecular Biology ,ABTS ,Biphenyl Compounds ,Gossypol ,technology, industry, and agriculture ,Povidone ,Water ,Free Radical Scavengers ,General Medicine ,Mitochondria ,Rats ,Oxidative Stress ,Liver ,chemistry ,Trolox ,Sulfonic Acids ,Oxidation-Reduction ,Oxidative stress - Abstract
The objective of this study is analysis of stability and antioxidant and antiradical activities of the gossypol derivative - megosin conjugated with N-polyvinylpyrrolidone (PVP). The results of study have shown the greater stability of megosin+PVP than megosin in aqueous solution of wide range of pH. Here we also demonstrated that megosin+PVP, named rometin, possess high antioxidant activity in the same range as well known antioxidant trolox as determined by its ability to scavenge free ABTS(+) and DPPH radicals in vitro. In addition, megosin+PVP was able to prevent accumulation of products of lipid peroxidation (thiobarbituric acid reactive substances and diene conjugates) and lysophospholipids formation in mitochondria membranes caused by CCl(4)-induced oxidative stress in rat liver in vivo. Furthermore, megosin+PVP rescued mitochondrial functions, such as respiration and oxidative phosphorylation, which declined after CCl(4) administration. Thus we present that the conjugation of megosin to PVP increase its stability and remain antioxidant activity in vivo and in vitro.
- Published
- 2012