1. Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate
- Author
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Soon-Jung Park, Myoung-Hee Ahn, Jung-Hyun Kim, Jae-Sook Ryu, Ik-Hwan Han, and Eun-Ah Park
- Subjects
musculoskeletal diseases ,medicine.medical_treatment ,Blotting, Western ,030231 tropical medicine ,Toxoplasma gondii ,Enzyme-Linked Immunosorbent Assay ,Inflammation ,Complex Mixtures ,Nitric Oxide ,Real-Time Polymerase Chain Reaction ,Brief Communication ,Immunoglobulin E ,030308 mycology & parasitology ,Microbiology ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,parasitic diseases ,cytokine ,medicine ,Humans ,Mast Cells ,Cells, Cultured ,0303 health sciences ,biology ,Chemistry ,biology.organism_classification ,Mast cell ,Interleukin 10 ,Infectious Diseases ,medicine.anatomical_structure ,Cytokine ,biology.protein ,Interleukin 12 ,Cytokines ,Parasitology ,medicine.symptom ,mast cell ,Toxoplasma - Abstract
The roles of mast cells in allergic diseases and helminth infections are well known. However, the roles of mast cells in T. gondii infection is poorly understood. This study was focused on the production of pro-inflammatory cytokines (TNF-α, IL-4), chemokines (CXCL8, MCP-1) and nitric oxide (NO) by mast cells in response to soluble lysate of T. gondii tachyzoites. Production of CXCL8 (IL-8), MCP-1, TNF-α and IL-4 were measured by RT-PCR and ELISA. Western blot were used for detection of CXCR-1 and CXCR2. Our results showed that T. gondii lysates triggered mast cells to release CXCL8, MCP-1, TNF-α, IL-4 and to produce NO. This suggests that mast cells play an important role in inflammatory responses to T. gondii.
- Published
- 2019