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Evaluation of the Korean isolate-1 tachyzoite antigen for serodiagnosis of toxoplasmosis
- Source :
- The Korean journal of parasitology. 46(1)
- Publication Year :
- 2008
-
Abstract
- To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
- Subjects :
- Adult
Male
Adolescent
Antigens, Protozoan
Enzyme-Linked Immunosorbent Assay
Rh strain
Brief Communication
Serology
Mice
Antigen
medicine
Animals
Humans
Serologic Tests
Korea
biology
Toxoplasma gondii
Middle Aged
biology.organism_classification
medicine.disease
Serum samples
Virology
Toxoplasmosis
Latex fixation test
Titer
Infectious Diseases
Parasitology
Female
Rabbits
Latex Fixation Tests
Subjects
Details
- ISSN :
- 00234001
- Volume :
- 46
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Korean journal of parasitology
- Accession number :
- edsair.doi.dedup.....45177155c6d20f52617fdd4c3733d1e8