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4 results on '"Mungiguerra, M"'

1. The changing epidemiology of carbapenemase-producing Klebsiella pneumoniae in Italy: Toward polyclonal evolution with emergence of high-risk lineages

Author

Di Pilato V., Errico G., Monaco M., Giani T., Del Grosso M., Antonelli A., David S., Lindh E., Camilli R., Aanensen D. M., Rossolini G. M., Pantosti A., Manso E., Pedna M. F., Mungiguerra M., Mosca A., Vailati F., Aschbacher R., Imbriani A., Sartore P., Giraldi C., Piana F., Pecile P., de Nittis R., Pini B., Mirri P., Bianchi E., Restelli A., Morelli D., Catania M. R., Barbaro A., Bernaschi P, Parisi G, Gualdi P, Dusi PA, Bona R, D'Andrea M M, Cavallo R, Lanzafame P, Sartor A, Grandesso S, Milano F, Di Pilato, V., Errico, G., Monaco, M., Giani, T., Del Grosso, M., Antonelli, A., David, S., Lindh, E., Camilli, R., Aanensen, D. M., Rossolini, G. M., Pantosti, A., Manso, E., Pedna, M. F., Mungiguerra, M., Mosca, A., Vailati, F., Aschbacher, R., Imbriani, A., Sartore, P., Giraldi, C., Piana, F., Pecile, P., de Nittis, R., Pini, B., Mirri, P., Bianchi, E., Restelli, A., Morelli, D., Catania, M. R., Barbaro, A., Bernaschi, P, Parisi, G, Gualdi, P, Dusi, Pa, Bona, R, D'Andrea, M M, Cavallo, R, Lanzafame, P, Sartor, A, Grandesso, S, and Milano, F

Subjects
Microbiology (medical), Imipenem, Klebsiella pneumoniae, Population, Microbial Sensitivity Tests, Meropenem, beta-Lactamases, Settore MED/07, Antibiotic resistance, Bacterial Proteins, Genotype, medicine, Humans, Pharmacology (medical), education, Pharmacology, Genetics, education.field_of_study, biology, Settore BIO/19, biology.organism_classification, Anti-Bacterial Agents, Klebsiella Infections, Resistome, Infectious Diseases, Italy, Multilocus sequence typing, Multilocus Sequence Typing, medicine.drug
Abstract

BackgroundPrevious studies showed that the epidemic of carbapenem-resistant Klebsiella pneumoniae (CR-KP) observed in Italy since 2010 was sustained mostly by strains of clonal group (CG) 258 producing KPC-type carbapenemases. In the framework of the National Antibiotic-Resistance Surveillance (AR-ISS), a countrywide survey was conducted in 2016 to explore the evolution of the phenotypic and genotypic characteristics of CR-KP isolates.MethodsFrom March to July 2016, hospital laboratories participating in AR-ISS were requested to provide consecutive, non-duplicated CR-KP (meropenem and/or imipenem MIC >1 mg/L) from invasive infections. Antibiotic susceptibility was determined according to EUCAST recommendations. A WGS approach was adopted to characterize the isolates by investigating phylogeny, resistome and virulome.ResultsTwenty-four laboratories provided 157 CR-KP isolates, of which 156 were confirmed as K. pneumoniae sensu stricto by WGS and found to carry at least one carbapenemase-encoding gene, corresponding in most cases (96.1%) to blaKPC. MLST- and SNP-based phylogeny revealed that 87.8% of the isolates clustered in four major lineages: CG258 (47.4%), with ST512 as the most common clone, CG307 (19.9%), ST101 (15.4%) and ST395 (5.1%). A close association was identified between lineages and antibiotic resistance phenotypes and genotypes, virulence traits and capsular types. Colistin resistance, mainly associated with mgrB mutations, was common in all major lineages except ST395.ConclusionsThis WGS-based survey showed that, although CG258 remained the most common CR-KP lineage in Italy, a polyclonal population has emerged with the spread of the new high-risk lineages CG307, ST101 and ST395, while KPC remained the most common carbapenemase.

Published
2021

2. Group A rotavirus surveillance before vaccine introduction in Italy, September 2014 to August 2017

Author

Ianiro G, Micolano R, Di Bartolo I, Scavia G, Monini M, Pagani E, Moroder L, Aschbacher R, Binda S, Pellegrinelli L, Farina C, Mignacca A, Bruno R, Vuolo A, Peyronel, Contarini MN, Zanella F, Bordignon G, Zoppelletto M, Affanni P, Lazzarotto T, Chiereghin, Recanatini C, D’Errico M, Camilloni, Concato C, Onori M, Valentini D, Campagnuolo, Mungiguerra M, Chironna M, Morea A, Castiglia P., Ianiro G, Micolano R, Di Bartolo I, Scavia G, Monini M, and Pagani E, Moroder L, Aschbacher R, Binda S, Pellegrinelli L, Farina C, Mignacca A, Bruno R, Vuolo A, Peyronel, Contarini MN, Zanella F, Bordignon G, Zoppelletto M, Affanni P, Lazzarotto T, Chiereghin, Recanatini C, D’Errico M, Camilloni, Concato C, Onori M, Valentini D, Campagnuolo, Mungiguerra M, Chironna M, Morea A, Castiglia P.

Subjects
0301 basic medicine, group A rotavirus, genotype, Settore MED/42 - Igiene Generale e Applicata, Reassortment, molecular methods, medicine.disease_cause, Group A, group A rotaviru, 0302 clinical medicine, Rotavirus, Genotype, Epidemiology, molecular method, 030212 general & internal medicine, acute gastroenteritis, Child, Antigens, Viral, infection control, Gastroenteritis, epidemiology, human, Italy, rotavirus, rotavirus infection, statistics, surveillance, viral infections, Child, Preschool, RNA, Viral, acute gastroenteriti, medicine.medical_specialty, statistic, Biology, Rotavirus Infections, 03 medical and health sciences, Virology, medicine, Humans, Routine screening, rotaviru, Public Health, Environmental and Occupational Health, Infant, Sequence Analysis, DNA, Acute gastroenteritis, Vaccine introduction, 030104 developmental biology, Sentinel Surveillance
Abstract

Introduction Group A rotaviruses (RVA) are the leading cause of acute gastroenteritis (AGE) in young children, causing ca 250,000 deaths worldwide, mainly in low-income countries. Two proteins, VP7 (glycoprotein, G genotype) and VP4 (protease-sensitive protein, P genotype), are the basis for the binary RVA nomenclature. Although 36 G types and 51 P types are presently known, most RVA infections in humans worldwide are related to five G/P combinations: G1P[8], G2P[4], G3P[8], G4P[8], G9P[8]. Aim This study aimed to characterise the RVA strains circulating in Italy in the pre-vaccination era, to define the trends of circulation of genotypes in the Italian paediatric population. Methods Between September 2014 and August 2017, after routine screening in hospital by commercial antigen detection kit, 2,202 rotavirus-positive samples were collected in Italy from children hospitalised with AGE; the viruses were genotyped following standard European protocols. Results This 3-year study revealed an overall predominance of the G12P[8] genotype (544 of 2,202 cases; 24.70%), followed by G9P[8] (535/2,202; 24.30%), G1P[8] (459/2,202; 20.84%) and G4P[8] (371/2,202; 16.85%). G2P[4] and G3P[8] genotypes were detected at low rates (3.32% and 3.09%, respectively). Mixed infections accounted for 6.49% of cases (143/2,202), uncommon RVA strains for 0.41% of cases (9/2,202). Conclusions The emergence of G12P[8] rotavirus in Italy, as in other countries, marks this genotype as the sixth most common human genotype. Continuous surveillance of RVA strains and monitoring of circulating genotypes are important for a better understanding of rotavirus evolution and genotype distribution, particularly regarding strains that may emerge from reassortment events.

Published
2019

3. Molecular epidemiology of KPC-producing Klebsiella pneumoniae from invasive infections in Italy: Increasing diversity with predominance of the ST512 clade II sublineage

Author

Conte, Viola, Monaco, Monica, Giani, Tommaso, D'Ancona, Fortunato, Moro, Maria Luisa, Arena, Fabio, D'Andrea, Marco Maria, Rossolini, Gian Maria, Pantosti, Annalisa, Bianchi, E., Catania, M. R., Cavalcanti, P., De Nittis, R., Dusi, P. A., Grandesso, S., Gualdi, P., Imbriani, A., Pini, B., Vincenzi, C., Meledandri, M., Agrappi, C., Parisi, G., Pollini, S., Restelli, A., Rocchetti, A., Vailati, F., Aschbacher, R., Barbaro, A., Bona, R., Chirillo, M., Corradini, S., Cuccurullo, S., De Bernochi, A., Dodi, C., Giammanco, A., Mencacci, Antonella, Milano, F., Miragliotta, G., Mungiguerra, M., Pedna, M. F., Piana, Francesca, Porcheddu, G. M., Rossi, M. R., Santino, I., Sartor, A., Sartore, P., Conte V., Monaco M., Giani T., D'Ancona F., Moro M.L., Arena F., D'Andrea M.M., Rossolini G.M., Pantosti A., Bianchi E., Catania M.R., Cavalcanti P., De Nittis R., Dusi P.A., Grandesso S., Gualdi P., Imbriani A., Pini B., Vincenzi C., Meledandri M., Agrappi C., Parisi G., Pollini S., Restelli A., Rocchetti A., Vailati F., Aschbacher R., Barbaro A., Bona R., Chirillo M., Corradini S., Cuccurullo S., De Bernochi A., Dodi C., Giammanco A., Mencacci A., Milano F., Miragliotta G., Mungiguerra M., Pedna M.F., Piana F., Porcheddu G.M., Rossi M.R., Santino I., Sartor A., Sartore P., Conte, V, Monaco, M, Giani, T, D'Ancona, F, Moro, Ml, Arena, F, D'Andrea, Mm, Rossolini, Gm, Pantosti, A, AR-ISS Study Group on Carbapenemase-Producing K., pneumoniae: Bianchi E, Catania, Mr, Cavalcanti, P, De Nittis, R, Dusi, Pa, Grandesso, S, Gualdi, P, Imbriani, A, Pini, B, Vincenzi, C, Meledandri, Alessio, Agrappi, C, Parisi, G, Pollini, S, Restelli, Ugo, Rocchetti, Romualdo, Vailati, F, Aschbacher, R, Barbaro, A, Bona, R, Chirillo, M, Corradini, S, Cuccurullo, S, De Bernochi, A, Dodi, C, Giammanco, Anna, Mencacci, A, Milano, F, Miragliotta, Giuseppe, Mungiguerra, M, Pedna, Mf, Piana, Daniela, Porcheddu, Gm, Rossi, Mr, Santino, I, Sartor, A, and Sartore, P.

Subjects
0301 basic medicine, Microbiology (medical), gel, Klebsiella pneumoniae, genotype, 030106 microbiology, Population, cross-sectional studies, pulsed-field, multilocus sequence typing, electrophoresis, gel, pulsed-field, humans, italy, klebsiella infections, klebsiella pneumoniae, molecular epidemiology, serogroup, beta-lactamases, genetic variation, Settore BIO/19 - Microbiologia Generale, Cross-Sectional Studies, Electrophoresis, Gel, Pulsed-Field, Humans, Italy, Klebsiella Infections, Molecular Epidemiology, Multilocus Sequence Typing, Serogroup, beta-Lactamases, Genetic Variation, Genotype, Pharmacology, Pharmacology (medical), Infectious Diseases, law.invention, Microbiology, beta-Lactamase, 03 medical and health sciences, law, Pulsed-field gel electrophoresis, Typing, education, Polymerase chain reaction, Cross-Sectional Studie, education.field_of_study, biology, Molecular epidemiology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Settore MED/07 - Microbiologia e Microbiologia Clinica, bacterial infections and mycoses, Virology, electrophoresis, Multilocus sequence typing, Human, Klebsiella Infection
Abstract

Objectives The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanita surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP). Methods A total of 17 laboratories distributed across Italy collected all consecutive non-replicate CR-KP isolated from invasive infections during two different study periods (2011-12 and 2013). Carbapenemase genes were searched for by filter hybridization and confirmed by PCR and sequencing. KPC-producing K. pneumoniae (KPC-KP) were typed by PFGE and MLST. Capsular types were identified by wzi gene typing. Results Of the collected K. pneumoniae isolates (n = 461), the overall proportion of CR-KP was 36.2% (n = 167). The majority (97%) of the CR-KP were positive for the blaKPC gene. Among the KPC-KP population, nine different STs were detected with the majority of isolates (94%) belonging to the clonal group (CG) 258. A subpopulation that belonged to ST512 and showed an identical PFGE profile represented the majority (57%) of KPC-KP strains, with a countrywide distribution. Capsular characterization showed the predominance of the wzi154, cps-2 capsular type (88.8% of all CG258 strains). ST258 strains were associated with both cps-1 and cps-2 capsular types, while ST512 was associated with cps-2 only. Conclusions Although a trend to a polyclonal evolution of the Italian KPC-KP was noted, this study showed that the KPC-KP population remained largely oligoclonal with the wide diffusion of an ST512 lineage carrying cps-2 capsular type and producing the KPC-3 enzyme.

Published
2016

4. [In vitro activity of voriconazole and other antifungal agents against clinical isolates of 138 Candida spp].

Author

Ricciardi AM, Ricciardi R, Danzi M, Mungiguerra M, Pisano L, and Marino A

Subjects
Amphotericin B pharmacology, Candida isolation & purification, Candidiasis microbiology, Drug Resistance, Fungal, Drug Resistance, Multiple, Fungal, Fluconazole pharmacology, Flucytosine pharmacology, Humans, Itraconazole pharmacology, Microbial Sensitivity Tests, Species Specificity, Voriconazole, Antifungal Agents pharmacology, Candida drug effects, Pyrimidines pharmacology, Triazoles pharmacology
Abstract

Voriconazole is used for treating invasive Aspergillosis, Fusarium and Scedosporium infections as well as resistant candidiasis. It is referred to as a second generation triazole. The purpose of this study was to evaluate the concordance of the results of antifungal voriconazole susceptibility tests for yeast isolates, comparing the Sensititre YeastOne method, Atb Fungus 3 and Etest. In all, 138 yeast isolates (42 C. tropicalis, 36 C. glabrata, 14 C. albicans, 8 C. famata, 6 C. parapsilosis, 4 C. dubliniensis, 3 C. krusei, 3 C. lusitaniae, 2 C. zeylanoides, 20 Candida spp.) were tested for susceptibility to amphotericin B, flucytosine, fluconazole , itraconazole and voriconazole with Atb Fungus 3 method. The concordance between the Sensititre YeastOne method, Atm Fungus 3 and Etest for voriconazole was high (90%).

Published
2009

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