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Molecular epidemiology of KPC-producing Klebsiella pneumoniae from invasive infections in Italy: Increasing diversity with predominance of the ST512 clade II sublineage
- Publication Year :
- 2016
-
Abstract
- Objectives The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanita surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP). Methods A total of 17 laboratories distributed across Italy collected all consecutive non-replicate CR-KP isolated from invasive infections during two different study periods (2011-12 and 2013). Carbapenemase genes were searched for by filter hybridization and confirmed by PCR and sequencing. KPC-producing K. pneumoniae (KPC-KP) were typed by PFGE and MLST. Capsular types were identified by wzi gene typing. Results Of the collected K. pneumoniae isolates (n = 461), the overall proportion of CR-KP was 36.2% (n = 167). The majority (97%) of the CR-KP were positive for the blaKPC gene. Among the KPC-KP population, nine different STs were detected with the majority of isolates (94%) belonging to the clonal group (CG) 258. A subpopulation that belonged to ST512 and showed an identical PFGE profile represented the majority (57%) of KPC-KP strains, with a countrywide distribution. Capsular characterization showed the predominance of the wzi154, cps-2 capsular type (88.8% of all CG258 strains). ST258 strains were associated with both cps-1 and cps-2 capsular types, while ST512 was associated with cps-2 only. Conclusions Although a trend to a polyclonal evolution of the Italian KPC-KP was noted, this study showed that the KPC-KP population remained largely oligoclonal with the wide diffusion of an ST512 lineage carrying cps-2 capsular type and producing the KPC-3 enzyme.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
gel
Klebsiella pneumoniae
genotype
030106 microbiology
Population
cross-sectional studies
pulsed-field
multilocus sequence typing
electrophoresis, gel, pulsed-field
humans
italy
klebsiella infections
klebsiella pneumoniae
molecular epidemiology
serogroup
beta-lactamases
genetic variation
Settore BIO/19 - Microbiologia Generale
Cross-Sectional Studies
Electrophoresis, Gel, Pulsed-Field
Humans
Italy
Klebsiella Infections
Molecular Epidemiology
Multilocus Sequence Typing
Serogroup
beta-Lactamases
Genetic Variation
Genotype
Pharmacology
Pharmacology (medical)
Infectious Diseases
law.invention
Microbiology
beta-Lactamase
03 medical and health sciences
law
Pulsed-field gel electrophoresis
Typing
education
Polymerase chain reaction
Cross-Sectional Studie
education.field_of_study
biology
Molecular epidemiology
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Settore MED/07 - Microbiologia e Microbiologia Clinica
bacterial infections and mycoses
Virology
electrophoresis
Multilocus sequence typing
Human
Klebsiella Infection
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....b0c8bed360e0ea2fa666a2bb5e37ce2e