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Abstract Background This Study investigated the role of WWTR1-AS1 in cervical squamous cell carcinoma (CSCC). Results WWTR1-AS1 expression was upregulated in CSCC tissues. WWTR1-AS1 was predicted to interact with miR-136, whereas correlation analysis revealed that there was no close correlation between WWTR1-AS1 and miR-136 across CSCC samples. Moreover, WWTR1-AS1 and miR-136 did not regulate the expression of each other. In addition, overexpression of WWTR1-AS1 increased the expression levels of Notch3, which could be targeted by miR-136. Cell stemness analysis indicated that the overexpression of WWTR1-AS1 and Notch3 increased CSCC cell stemness and the capacity of CSCC cell to grow as spheroids. Overexpression of miR-136 decreased CSCC cell stemness and reversed the effects of overexpression of WWTR1-AS1 on Notch3 in CSCC cells. Conclusion Therefore, WWTR1-AS1 may upregulate Notch3 through miR-136 to increase cancer cell stemness in CSCC.

BackgroundAbnormal cerebrospinal fluid (CSF)/serum albumin ratio (Qalb) levels have been observed in patients with cognitive impairment. Few studies have specifically focused on Lewy Body Disease (LBD), and the results were controversial. Thus, we conducted this systematic review and meta-analysis to investigate Qalb levels in patients with LBD by including data from different studies.MethodWe systematically searched PubMed, Embase, Cochrane Library, and Web of Science databases for a collection of studies containing studies comparing Qalb levels in patients with LBD and healthy controls (including healthy controls and other dementia subtypes). In the initial search, 86 relevant papers were retrieved. Standardized mean differences (SMD) in Qalb levels were calculated using a random effects model.ResultsA total of 13 eligible studies were included. Mean Qalb levels were significantly higher in patients with LBD compared to healthy older adults [standardized mean difference (SMD): 2.95, 95% confidence interval (CI): 0.89–5.00, Z = 2.81, p = 0.005]; and were significantly higher in patients with LBD than in patients with Alzheimer’s disease (AD) (SMD: 1.13, 95% CI: 0.42–1.83, Z = 3.15, p = 0.002);whereas mean Qalb levels were significantly higher in patients with frontotemporal lobar degeneration (FTLD) compared to those with AD (SMD: 1.13, 95% CI,0.14–2.13, Z = 2.24, p = 0.03).ConclusionQalb levels were significantly elevated in LBD patients compared with normal older adults and were higher than those in AD patients and FTLD patients, which helped in the differential diagnosis of LBD from other neurodegenerative diseases.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024496616.

Display rules specify socially appropriate facial expressions in a given situation. However, managing emotions for such a social adaption sometimes leads to deleterious psychological outcomes. Given that people nowadays rely on emojis to express emotions online, the present study investigated (1) whether display rules exist in emoji communications and (2) how emotion management using emojis is associated with psychological well-being. Prior studies have demonstrated the effects of context on the frequency of emoji use. However, the intensity and type of expression may differ, even if emojis are used at the same frequency. The current study thus investigated whether emotional expressions and the types of emojis used are adjusted to contexts similar to facial displays. As many as 1,289 Japanese participants typed emojis in response to Internet chats and reported the intensity of their emotional expressions. The contexts of the chats varied depending on the target of use, the emotional value of contexts, and private or public settings. The results showed that, similar to facial displays, individuals expressed emotions through emojis more with those closely related, more in positive contexts than in negative contexts, and more in private than in public contexts. When the expressions were intense, individuals used emojis consistent with the emotional value of the context. Upon attenuating the expressions, this study found that individuals tended to use euphemistic emojis and sent smiling emojis in negative contexts to manage the expressions. Moreover, expressing emotions with emojis was associated with subjective well-being, whereas managing emotions with emojis was weakly associated with depressive symptoms. Together, this study indicates the existence of display rules for emojis, calling for future research on the psychological impact of online emotion norms.