1. How to make and trap a pseudo-vesicle with a micropipette
- Author
-
Tapie, Pierre, Prevost, Alexis M., Montel, Lorraine, Pontani, Léa-Laetitia, and Wandersman, Elie
- Subjects
Condensed Matter - Soft Condensed Matter ,Physics - Biological Physics ,Physics - Instrumentation and Detectors - Abstract
We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of {\alpha}-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
- Published
- 2022