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How to make and trap a pseudo-vesicle with a micropipette
- Publication Year :
- 2022
-
Abstract
- We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of {\alpha}-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
Details
- Database :
- arXiv
- Publication Type :
- Report
- Accession number :
- edsarx.2207.06402
- Document Type :
- Working Paper