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1. Supercritical fluid extraction and reversed-phase liquid chromatography methods for vitamin A and β-carotene heterogeneous distribution of vitamin A in the liver

Author

Monica R. Wong, Terry R. Neidlinger, Annie O. Lo, Cynthia Kwan, and Betty J. Burri

Subjects
Vitamin, medicine.medical_treatment, Biochemistry, High-performance liquid chromatography, Antioxidants, Analytical Chemistry, chemistry.chemical_compound, medicine, Animals, Vitamin A, Chromatography, High Pressure Liquid, Chromatography, Organic Chemistry, Carotene, Supercritical fluid extraction, Retinol, Reproducibility of Results, General Medicine, Reversed-phase chromatography, beta Carotene, Supercritical fluid, Liver, chemistry, Cattle, Spectrophotometry, Ultraviolet, Quantitative analysis (chemistry)
Abstract

We developed supercritical fluid procedures for extracting vitamin A and beta-carotene from vitamin supplements and calf liver tissue. The SF extracts could be injected onto an HPLC column without further pretreatment. Samples were analysed by RP-HPLC using diode array detection or by spectrophotometry. Recoveries were very good. SF extracts from a vitamin preparation of uniform composition had an R.S.D. of 4%. Extracts from calf liver supplements were predictably more heterogeneous. The SF extraction method is less labor intensive then traditional liquid-liquid procedures for extracting vitamin A and carotenoids from tissues.

Published
1997

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