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Supercritical fluid extraction and reversed-phase liquid chromatography methods for vitamin A and β-carotene heterogeneous distribution of vitamin A in the liver
- Source :
- Journal of Chromatography A. 762:201-206
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- We developed supercritical fluid procedures for extracting vitamin A and beta-carotene from vitamin supplements and calf liver tissue. The SF extracts could be injected onto an HPLC column without further pretreatment. Samples were analysed by RP-HPLC using diode array detection or by spectrophotometry. Recoveries were very good. SF extracts from a vitamin preparation of uniform composition had an R.S.D. of 4%. Extracts from calf liver supplements were predictably more heterogeneous. The SF extraction method is less labor intensive then traditional liquid-liquid procedures for extracting vitamin A and carotenoids from tissues.
- Subjects :
- Vitamin
medicine.medical_treatment
Biochemistry
High-performance liquid chromatography
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
medicine
Animals
Vitamin A
Chromatography, High Pressure Liquid
Chromatography
Organic Chemistry
Carotene
Supercritical fluid extraction
Retinol
Reproducibility of Results
General Medicine
Reversed-phase chromatography
beta Carotene
Supercritical fluid
Liver
chemistry
Cattle
Spectrophotometry, Ultraviolet
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 762
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....01260a934b386c447c212b8574c1fb4b