109 results on '"Mohsen Arbabi"'
Search Results
2. In vitro antileishmanial activity of ozone against Leishmania major
- Author
-
Ali Salmani, Mahdi Delavari, and Mohsen Arbabi
- Subjects
ozone ,leishmania major ,apoptosis ,anti-leishmanial ,Medicine (General) ,R5-920 - Abstract
Objectives: This research aimed to investigate the impact of ozone on Leishmania major.Methods: Leishmania major promastigotes were exposed to ozone flow at varying concentrations (5, 10, 20, 30, and 40 ppm) for different durations (5, 10, 20, 30, and 40 minutes). The DNA of the treated promastigotes was extracted to evaluate DNA fragmentation. Apoptosis induction in the treated L. major promastigotes was analyzed using a specific kit. Additionally, scanning electron microscopy was employed to observe ultrastructural changes in the treated promastigotes.Results: The highest fatality rate (100%) occurred after 30 minutes of exposure to 40 ppm ozone gas, while the lowest fatality rate was 16.14% after 5 minutes of exposure to 5 ppm ozone gas. The DNA of the treated parasites was degraded, and significant morphological changes were observed in the body and flagella of the promastigotes. Induction of apoptosis was also noted.Conclusion: Ozone exhibits anti-Leishmania activity and induces apoptosis in Leishmania major. Furthermore, clear ultrastructural changes were observed in Leishmania major following ozone exposure. These findings suggest that ozone warrants further investigation as an anti-leishmanial agent. more...
- Published
- 2024
- Full Text
- View/download PDF
Catalog
3. Morphological Characterization and Genotyping of Acanthamoeba Isolates From Oral and Nasal Samples of Cancer Patients in Kashan, Iran
- Author
-
Sima Rasti, Tayebeh Taghipour, Mahdi Delavari, Hossein Hooshyar, Gholam Abbas Moosavi, and Mohsen Arbabi
- Subjects
Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Conclusion: The prevalence of Acanthamoeba infection in oral and nasal cancer patients was higher in Kashan, Iran, compared to other countries. Due to the high rate of oral Acanthamoeba contamination, oral sampling is recommended for better detection of this protozoan. Since T4 is the predominant genotype that can cause serious diseases in high-risk groups, increasing physicians’ awareness of infections associated with Acanthamoeba and preventive and control measures are strongly suggested. more...
- Published
- 2024
- Full Text
- View/download PDF
4. Antileishmanial activity of auranofin against Leishmania major in vitro
- Author
-
Mohammad Ezati Mehmandust Olya, Mahdi Delavari, Mohsen Arbabi, Sima Rasti, Hossein Hooshyar, and Morteza Salimian
- Subjects
apoptosis ,auranofin ,fragmentation ,leishmania major ,ultrastructural ,Medicine (General) ,R5-920 - Abstract
Aim: Despite the various studies done in the field of cutaneous leishmaniasis treatment, there is still no perfect and safe drug for definite treatment of this disease. Therefore, the quest to find an appropriate drug continues. Previous studies have shown that auranofin has anti-leishmanial activity; therefore, in this study; the effect of auranofin on Leishmania major was studied. Materials and Methods: Effectiveness of four concentrations (1, 2, 4, and 8 μg/ml) of auranofin on L. major was studied. After culturing amastigotes and promastigotes of the parasite, IC50 was calculated. The ability of auranofin to induce apoptosis in promastigotes was evaluated, and the degree of fragmentation of promastigotes DNA after treatment with auranofin was studied. Subsequently, the ultrastructural changes induced by treatment with auranofin in promastigotes were studied. Using the obtained results, IC50 of auranofin against amastigotes and promastigotes was calculated as 1.007 and 2.38 μg/ml, respectively. Findings: Showed that auranofin induce apoptosis in L. major. The highest rate of apoptosis (%80.1) occurred at the concentration of 8 μg/ml and also auranofin-induced fragmentation of DNA. Considerable changes occurred in the shape of body and free flagellum of the L. major promastigotes after treatment with auranofin. Conclusion: Based on the results, it can be concluded that auranofin has a considerable anti-leishmanial activity and additional studies in this field will be based on the results. more...
- Published
- 2022
- Full Text
- View/download PDF
5. Molecular identification and frequency of cyst-forming coccidia (Sarcocystis, Toxoplasma gondii, and Neospora caninum) in native slaughtered cattle in Kashan, Central Iran
- Author
-
Hossein Hooshyar, Feresthte Chehrazi, and Mohsen Arbabi
- Subjects
cattle ,iran ,kashan ,neospora caninum ,sarcocystis ,toxoplasma gondii ,Medicine (General) ,R5-920 - Abstract
Aim: Cattle is one of the main sources of food supply chain for humans in most countries. The present study aimed to identify the infection rates for Toxoplasma gondii, Neospora caninum, and Sarcocystis spp. by polymerase chain reaction (PCR) method in native slaughtered cattle in Kashan, central Iran. Materials and Methods: Totally, 159 diaphragm, esophagus, and muscle samples (53 samples of each) were collected from native beef cattle from Kashan slaughterhouse, central Iran. The genomic DNA was extracted, and PCR method was used separately for detection of N. caninum, Sarcocystis, and T. gondii species using specific primers. Finding: Sarcocystis was found in 84.9% of muscles, 83% of esophagus, and 84.9% of diaphragm samples. Mixed infection (Sarcocystis cruzi–Sarcocystis hominis) was the most common infection, followed by S. cruzi and S. hominis. Sarcocystis hirsuta was not detected in any samples. T. gondii was detected only in three (5.7%) out of the 53 muscle tissues samples of cattle. N. caninum was found in 18.9% of muscles, 24.5% of esophagus, and 28.3% of diaphragm samples. One of the cattle had coinfection to Neospora, Toxoplasma, and Sarcocystis in muscles simultaneously. There was no statistically significant difference between infection rates and age as well as sex in each organ. Conclusion: This study revealed a low prevalence rate of T. gondii, but a high prevalence of infection to N. caninum and S. cruzi or mixed infection of S. cruzi with S. hominis among slaughtered cattle. Prevention measures such as keeping away dogs from cattle grazing are recommended. more...
- Published
- 2021
- Full Text
- View/download PDF
6. Evaluation of anti-helmintic activity of Zingiber officinale roscoe extract on Fasciola hepatica miracidia In vitro
- Author
-
Atefe Ghafar, Mohsen Arbabi, Mahdi Mosayebi, Hossein Hooshyar, and Ali Mohammad Nickfarjam
- Subjects
anti-miracidia activity ,fasciola hepatica ,hatch ability ,zingiber officinale roscoe ,Medicine (General) ,R5-920 - Abstract
Aims: The control of fascioliasis has depended on the utilization of a predetermined number of anthelmintic drugs. Nonetheless, the resistance of antifasciolid is presently boundless, and there is a need for potential drug properties of medicinal plants as new medications against Fasciola spp. to interrupt the parasite transmission. Materials and Methods: This investigation is meant to assess the potential anti-fasciolicide impacts of Zingiber officinale roscoe hydroalcoholic extract against the Fasciola miracidia. The eggs of Fasciola hepatica were gathered from the livers contaminated goats and sheep and washed 20 times with chlorine-free water, and afterward incubated at different pH, temperature, and light density for embryonic development. Concentrated miracidia of Fasciola spp. were incubated at different concentrations (2, 5, and 10 μg/mL) of hydroalcoholic extract of Z. officinale at different time intervals. The data were analyzed using the ANOVA statistical test. Results: The extract at different concentrations showed antifasciolid effects in comparison to triclabendazole. This braking action was dose-proportional and further related firmly to the disclosure time ( P < 0.001). In concentration of 10ug/ml extract killed the parasites at 105 ± 3 s ( P < 0.001). Conclusion: In vitro antimiracidia activities of the hydro alcoholic extract of Z. officinale was satisfactory and potent fasciolicide effective, however, in vivo impact of this extract, remains for extra assessment. In this manner, these therapeutic plant extracts might be seen as confident origins of bioactive composites that could be matured against miracidia. This is the main work to evaluate the potential enemy of fasciolicide impact of certain plants against Fasciola miracidia. more...
- Published
- 2021
- Full Text
- View/download PDF
7. Genotypes Identification of echinococcus granulosus isolated from iranian dogs and camels using three polymerase Chain reaction-based methods of cox1 gene
- Author
-
Mohsen Arbabi, Hossein Hooshyar, Mahdi Delavari, and Nader Pestechian
- Subjects
camel ,cox1 gene ,dog ,echinococcus granulosus ,high resolution melting ,polymerase chain reaction-restriction fragment length polymorphism ,sequencing ,Medicine (General) ,R5-920 - Abstract
Aims: Hydatidosis is an important zoonotic disease that is caused by a tiny tapeworm, namely Echinococcus granulosus. In this study, three polymerase chain reaction (PCR)-based methods, including, high resolution melting (HRM) analysis, DNA sequencing, and PCR-restriction fragment length polymorphism (RFLP) have been used for genotype the identification of E. granulosus isolates from dogs and camels in Zarinshahr and Najafabad, Isfahan province, Iran. Materials and Methods: A total of 200 adult worms of 40 dogs and 51 samples of camel hydatid cysts were examined. Molecular characterization of isolates was performed using HRM assay, sequencing of DNA, and digestion Rsa1 pattern coding for the mitochondrial cox1 gene. For analysis of the HRM melting curve, we used the Tm within the range of 77.50°C–79.23°C. Results: HRM analysis revealed that 72.5%, 15%, and 12.5% dog's genotypes and 41.17%, 21.56%, and 35.29% camel genotypes were G1, G3, and G6, respectively. PCR-RFLP analysis, spare parts 310 bp and 138 bp of cox1 that shows the G1 genotype in all of the isolates. Sequence analysis as well as HRM assay was confirmed genotypes of G1, G3, and G6 in camels and dogs. Based on three methods of the cox1 gene the dominant genotype was G1. Conclusion: The PCR-RFLP only identified the G1 genotype, whereas the HRM analysis, as well as DNA sequencing, were detected three genotypes G1, G3, G6, therefore, these two methods have enough accuracy for the determination of genotypes of E. granulosus. This information leads to a better understanding of the biological characteristics of E. granulosus genotypes in Iran and shows the camel as a source of human hydatidosis. more...
- Published
- 2021
- Full Text
- View/download PDF
8. Subtypes identification and frequency of Blastocystis isolated from patients in Kashan, Central Iran
- Author
-
Shirin Khodabakhsh Arbat, Hossein Hooshyar, Mohsen Arbabi, Mahdi Delavari, Sima Rasti, and Gholamabbas Mousavi
- Subjects
blastocystis ,human ,iran ,kashan ,polymerase chain reaction ,subtype ,Medicine (General) ,R5-920 - Abstract
Aims: Blastocystis species are one of the most common enteric protist infections in humans and some animals worldwide. Molecular studies have shown that there is a high level of genetic variation among Blastocystis isolates. The aim of this study was to identify the subtypes and frequency of Blastocystis isolates in patients who referred to the medical diagnostic laboratories in Kashan, Central Iran. Materials and Methods: This cross-sectional study was conducted on 1118 patients, from December 2017 to June 2018. Fecal specimens were evaluated by the microscopic examination. Positive samples were cultivated in Robinson media. After massive growth and DNA extraction, a 550 bp from the small subunit ribosomal RNA gene was amplified by the polymerase chain reaction (PCR) for subtype identification. The PCR products have been sequenced, identified, and compared at the NCBI site. The results were analyzed using the SPSS software version 16. Results: The frequency of Blastocystis sp. was 8.58%, (confidence interval = 6.94%–10.22%) from which 76% were men and 24% were women. Of the 51 PCR positive samples, ST3 (41.2%), ST1 (39.2%), ST2 (11.8%), and 7.8% isolates were identified as mixed. ST3 and ST1 have been more common. The highest levels of infection were observed in the food-handlers, the age group of 31–40, and people with high school education. Conclusion: The results showed that the frequency of Blastocystis was lower than other studies and the most common Blastocystis subtype was subtype 3, followed by subtype 1, and subtype 2. more...
- Published
- 2021
- Full Text
- View/download PDF
9. Maintenance of liver fluke, Dicrocoelium dendriticum, outside the body of its native host
- Author
-
Mohsen Arbabi, Atefeh Hadad, Hossein Hooshyar, Hossein Akbari, and Seyed Mostafa Hosseinpour Mashkani
- Subjects
culture media ,dicrocoelium dendriticum ,in vitro ,roswell park memorial institute medium ,survival rate ,Medicine (General) ,R5-920 - Abstract
Aims: In vitro cultivation of digenea would help the development of effective treatments and studies of the biology of the parasites. The goal of the present study was to optimize culture conditions for the maintenance liver fluke, Dicrocoelium dendriticum. Materials and Methods: Forty fresh D. dendriticum were collected from the sheep liver and washed three times with warm Roswell Park Memorial Institute (RPMI) 1640 Medium. The collected worms were transferred to 24-well Nunc-Immuno plates containing RPMI media supplemented with 50% of fetal bovine serum (FBS), 2% of sheep red blood cells (RBCs), 50 IU/ml of penicillin, and 50 mg/ml streptomycin. The mobility of the live/dead worms was observed by inverted microscope. The mean and median survival time was calculated by Kaplan–Meier model, and survival and hazard function graphs were also analyzed. Results: D. dendriticum was lived in vitro only for long periods of about 25 dyes. The 1st day of maintaining in culture media, one worm was dead and the number of dead worms was raised to 40 after 25 days of incubation. On the one hand, the mean survival time was 392 h with a confidence interval (CI) of 95% (384.8–400.03). On the other hand, the median survival time was 420 h with a CI of 95% (406.9–433.09). D. dendriticum was able to be alive in RPMI 1640 media for at least 25 days. Conclusion: RPMI 1640 supplemented with FBS, and RBCs can be used as short-term maintenance for the in vitro culture of D. dendriticum. The outcomes of the current study could be useful for many aspects of parasitological analysis. more...
- Published
- 2021
- Full Text
- View/download PDF
10. Bioremediation of Polycyclic Aromatic Hydrocarbons in Contaminated Soils Using Vermicompost
- Author
-
Fazel Mohammadi-Moghadam, Ramazan Khodadadi, Morteza Sedehi, and Mohsen Arbabi
- Subjects
Chemical engineering ,TP155-156 - Abstract
Bioremediation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soils are reported in many literatures. Composting, in addition to bioremediation, can simultaneously increase soil organic matter content and soil fertility and is thus regarded as one of the most cost-effective methods of soil remediation. In this study, biodegradation of phenanthrene (PHE) and pyrene (PYR) is reported by microbial consortia enriched by vermicompost. After soil samples preparation and grinding, the samples were contaminated with 100, 200, and 300 mg/kg of PHE and PYR concentrations and inoculated with three concentrations (2, 4, and 6 wt.%) of vermicompost. PHE and PYR concentrations were analyzed by HPLC during bioremediation. After 70 days, two highly capable microbial consortia were used to remove the pollutants in bioaugmentation conditions. Analysis of their microbial composition revealed that the consortia contain several Proteobacteria phylum bacterial species, and the most common genera were Pseudomonas and Citrobacter. Decontamination rates for PHE and PYR were estimated to be 89% and 83% over 45 days, respectively. Biodegradation kinetics revealed that microbial degradation followed a first-order kinetics. This study provides clear evidence on the biodegradation of PHE and PYR, paving the way for the development of bioremediation technologies for the recovery of polluted ecosystems. more...
- Published
- 2022
- Full Text
- View/download PDF
11. Epidemiology and economic loss of fasciolosis and dicrocoeliosis in Arak, Iran
- Author
-
Mohsen Arbabi, Elnaz Nezami, Hossein Hooshyar, and Mahdi Delavari
- Subjects
dicrocoeliosis ,economic loss ,epidemiology ,fasciolosis ,Iran ,slaughtered animal ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Aim: Fasciolosis and dicrocoeliosis are important parasitic diseases worldwide, causing significant financial losses due to decrease in production and viscera condemnation in animals. We performed the current research to assess the epidemiology of these infections and determine their significance from an economic perspective in Arak, Iran. Materials and Methods: In total, we evaluated 118,463 sheep, 207,652 goats, and 43,675 cattle through necropsic analysis at the slaughterhouses. The average weight of sheep, goat, and cattle liver was 1000, 900, and 5000 g, respectively. The average price of liver in the market was 8 USD/kg. Moreover, the elimination of fundamental nutrients and vitamins was evaluated in infected livers. The prevalence of fasciolosis and dicrocoeliosis was determined. Analysis of variance test was applied for the statistical analysis, and the significance level was more...
- Published
- 2018
- Full Text
- View/download PDF
12. Evaluation of anti-Neospora caninum antibody presence in cow's milk in Kashan
- Author
-
Mohammadreza Hadadi, Reza Sherafati, Mahdi Delavari, Mohsen Arbabi, Hamidreza Gilasi, and Alireza Abed
- Subjects
Neospora caninum ,Cow's milk ,Iran ,Medicine (General) ,R5-920 - Abstract
Background: Neosuporosis is caused by a parasitic protozoan called Neospora caninum. This parasite can infect different species of domestic and wild animals. Some studies have reported the possibility of human infection with this parasite. The aim of this study was to evaluate the anti-Neospora caninum antibody in raw milk of cattle in Kashan city. Materials and Methods: In this study, 187 milk samples were collected from two industrial farms in Kashan. The breed of all cows was Holstein. Before sampling, the livestock information such as age, race, history of abortion and stillbirth, the daily milk yield was recorded in the prepared forms. Fat of the samples was separated by centrifuge and lactoserums were collected. To determine anti-Neospora caninum antibody in milk samples, an indirect ELISA kit was used. Results: Results showed that 18.8% (CI=18.18±5.53) of the samples were infected with Neospora caninum. No significant relationship was found between the infection with this protozoa and milk production and age of the cows (P=0.84). Also, the results showed that there was no significant relationship between abortion and infection with Neospora. Conclusion: Considering the contamination rate of cow's milk in Kashan, the implementation of control programs in livestock has great importance. Also, considering the possibility of human infection with Neospora, the need for attention to these control programs is more and more evident. more...
- Published
- 2018
13. Source Identification and Ecological Risk Assessment of Polycyclic Aromatic Hydrocarbons in Surface Sediments )Case Study: Pars Special Economic Energy Zone
- Author
-
Parisa Hoseyn Khezri, Masoud Hatami Manesh, Arash Haghshenas, Mohsen Mirzaei, Mohsen Arbabi, and Behzad Mohammadi Bardkashki
- Subjects
polycyclic aromatic hydrocarbons compounds ,ecological risk assessment ,surface sediment ,toxicity ,pars special economic energy zone ,Medicine ,Medicine (General) ,R5-920 - Abstract
Background and purpose: Polycyclic aromatic hydrocarbons (PAH) compounds are amongst the most hazardous environmental pollutants due to some features such as chemical stability, low degradation, bioaccumulation, and high toxicity for living organisms. Their resources are found in Persian Gulf beaches, so, this study aimed at investigating the concentration, source identification, and ecological risk of PAH compounds in coastal sediments in Pars Special Economic Energy Zone (PSEEZ), Iran. Materials and methods: In order to assess the concentration of PAHs, 16 stations along the coast were selected and in each station two samples were collected. After preparation and extraction of samples using Soxhlet, the concentrations of these compounds were determined by HPLC. The toxicity evaluation indicators and diagnostic ratios were used to assess the ecological risk and determining their origin. Results: Mean concentration of PAHs in sediment was 292.72±54.44 ng/g dry weight. The mean effects range-median quotient of the PAHs (M-ERM-Q, M-PEL-Q), toxic equivalence quotient (TEQ), and mutagenic equivalence quotient (MEQ) showed that current values were less than the permissible limit. Comparison of PAHs concentration with sediment quality standards (NOAA, SQGs, and EPA) showed that the values for all compounds were lower than the standard limits (PEC, TEC, PEL, TEL, ERM). However, among these compounds, the average concentration of Acl was higher than the ERL standard and the concentrations of Acl, Ace, and Phe were higher than the EPA toxicity threshold. Conclusion: In this study, moderate PHAs pollution was seen in the region. Both petrogenic and fuel sources were found to be involved in introduction of these compounds into coastal sediments depending on the location of the station and its sources of contamination. Also, in terms of ecological risk, these compounds have low risks for living organisms. more...
- Published
- 2018
14. Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran
- Author
-
Simin Bari, Mohsen Arbabi, Pooria Gill, Mehdi Sharif, Hajar Ziaei Hezarjaribi, Samira Dodangeh, Abbas Alizadeh, Zeinab Hedayati, and Shahabeddin Sarvi
- Subjects
dicrocoelium ,morphology ,28s rdna ,pcr-rflp ,Medicine ,Medicine (General) ,R5-920 - Abstract
Background and purpose: Dicrocoeliasis is a prevalent parasite in ruminants caused by different species of Dicrocoelium spp. This parasite lives in bile duct and gallbladder of ruminants and accidentally in human. The economic significance of dicroceliasis is due to direct damage to the liver and, consequently, to the loss of significant amounts of protein. The present study aimed to investigate the morphometric and molecular (PCR and PCR-RFLP) parameters of Dicrocoelium species isolates from sheep, goat, and cattle in Mazandaran province, Iran during 2013-2015. Materials and methods: In this descriptive study, 50 trematodes isolated from livers of sheep (n= 20), goat (n= 20), and cattle (n= 10) were collected form abattoirs in Mazandaran province. Adult worms were studied by morphometric and molecular methods on 28s rDNA. The RFLP technique was used to identify the genus. Some samples were also sequenced. Results: All isolates of three hosts (sheep, goat, and cattle) showed significant differences in many morphological parameters. According to characterization of testicles that is a remarkable characteristic for differential diagnosis of Dicrocoelium species, they were in tandem position in all isolates. Moreover, no significant difference was observed in their size in different hosts. Four bands, including 116, 145, 393, and 409 bp were produced from PCR method after treatment by enzyme. In molecular technique, there were no any significant differences but in morphometric analysis, some differences were found. Conclusion: The molecular and morphometric results indicated that D. dendriticum is the only species infecting sheep, goat, and cattle in Mazandaran province. more...
- Published
- 2018
15. Electrocoagulation process using aluminum electrodes for treatment of baker's yeast industry wastewater
- Author
-
Mohsen Arbabi, Samaneh Shafiei, Sadeghi Mehraban, Abbas Khodabakhshi, Ashkan Abdoli, and Arman Arbabi
- Subjects
aluminum electrode ,baker's yeast wastewater ,electrocoagulation ,treatment ,Environmental technology. Sanitary engineering ,TD1-1066 ,Environmental engineering ,TA170-171 ,Environmental sciences ,GE1-350 - Abstract
Background and Aims: Severe contamination with organic compounds and very high color is characteristic of yeast industry wastewater. Discharging this wastewater into the environment has adverse effects on the environment. The present study was conducted to determine the efficiency of the electrocoagulation (EC) using aluminum electrodes for the removal of color, turbidity, and chemical oxygen demand (COD) from the baker's yeast industry wastewater. Materials and Methods: In this experimental study, the effect of current densities (60, 80, 100, and 120 A/m2) and reaction times (15, 30, 45, and 60 min) using aluminum electrode was investigated on removal efficiencies of COD, color, and turbidity. The pilot consisted of a reactor with a useful volume of 2.5 l of epoxy glass, a direct current power supply, and aluminum electrodes of 8 cm × 8 cm in diameter. Results: The highest removal efficiencies were obtained to be 83% for COD, 93% for color, and 96% for turbidity at density of 80 A/m2 and 45-min contact time (pH = 7). Under these conditions, the power and electrode consumption was 16.89 kWh and 94.3 g/m3, respectively, and the treatment cost of wastewater was estimated to be 1.5 $ per each cubic meter. Conclusion: The results showed that EC process using aluminum electrode is an appropriate and effective method for removing color, turbidity, and COD from baking industry wastewater. more...
- Published
- 2022
- Full Text
- View/download PDF
16. The effect of alcoholic extracts of Arctium lappa L. and Satureja hortensis L. against Trichomonas vaginalis in vitro
- Author
-
Mohsen Arbabi, Zohre Fakhrieh-Kashan, Mahdi Delavari, Mohsen Taghizadeh, and Hossein Hooshyar
- Subjects
Trichomonas vaginalis ,Alcoholic extract ,Satureja hortensis L. ,Arctium lappa L. ,Medicine (General) ,R5-920 - Abstract
Background: Trichomonas vaginalis infection is one of the most prevalent type of vaginitis in women. Considering the side effects of metronidazole and therapeutic properties of Arctium lappa L. and Satureja hortensis L. in traditional medicine, this study aimed to examine the anti-Trichomonas effects of Arctium lappa and Satureja hortensis alcoholic extracts in vitro. Materials and Methods: This experimental study was conducted on T. vaginalis isolated from 1203 persons referred to five health centers in Kashan city. Five T. vaginalis isolates were cultured in a TYI-S-33 medium and were used to study the effect of Arctium lappa and Satureja hortensis extracts. The effects of different concentrations (12.5, 25, 50, 100, 200, 400, 800 and 1000 µg/mL) of plant extracts on the growth of T. vaginalis trophozoeites were studied 12, 24, and 48 h after the culture. Also, the culture media and metronidazole (0.025, 0.05, 0.1, 0.2, 0.4 µg/mL) were considered as the negative and positive controls, respectively. The effects of the extracts and drug were examined by counting the number of live and dead parasites using the trypan blue staining method. Results: Results showed that the alcoholic extracts of Satureja hortensis and Arctium lappa had an inhibitory effect on the growth of T. vaginalis. The IC50 values of the alcoholic extracts of Satureja hortensis, Arctium lappa and metronidazole after 24 h were 190.8, 996.7 and 0.0326 µg/mL, respectively. Conclusion: The present study shows the in vitro anti-Trichomonas effect of Arctium lappa and Satureja hortensis extracts. The anti-Trichomonas effect of Satureja hortensis extract was higher than that of the Arctium lappa extract. Further studies are recommended to investigate the anti-Trichomonas effect of major components of these plants, especially the Satureja hortensis extract. more...
- Published
- 2017
17. Survey of hydatid cyst surgeries in hospitals affiliated to Ahvaz Jundishapur University of Medical Sciences during 2004 to 2014
- Author
-
Mohamad Jaafar Yad Yad, Sanaz Nasiri, Mahdi Delavari, and Mohsen Arbabi
- Subjects
Hydatid cyst ,Echinococcus granulusos ,Medicine (General) ,R5-920 - Abstract
Background: Hydatidosis is one of the most important zoonotic diseases with global distribution. Due to the prevalence of the disease in country, the study aimed to determine the clinical and laboratory findings in patients with hydatidosis admitted to surgical departments of educational hospitals, Imam Khomeini and Golestan, in Ahvaz city during 2004-2014. Materials and Methods: Data of the patients including demographic characteristics, and clinical and laboratory findings were recorded in a prepared form and analyzed using descriptive statistics and Chi-square tests by the SPSS software. Results: From a total of all patients, 49 (55.7%) and 39 patients (44.3%) were females and males respectively; 25 patients were resident in rural areas and 62 in urban areas. The mean age of the patients was 30.6±2 years. Also, the maximum and minimum percentages of the disease were observed in the age group 20-29 and 80-89, respectively. Recurrence was observed in 9 patients. The highest prevalence of the hydatid cyst was in the right lobe of the liver (26.1%). Among the methods of imaging, sonography and MRI had the highest and lowest use in the diagnosis of the disease, respectively. Conclusion: Findings of this study show that the most common organs affected are the liver and the younger age group and suburbs are more prone to hydatidosis. Due to the chronicity of the disease and the late diagnosis during the visit, screening for endemic areas and high-risk persons is recommended. more...
- Published
- 2017
18. Molecular and genotyping identification of Echinococcus granulosus from camel and dog isolates in Isfahan, Iran (2015-2016)
- Author
-
Mohsen Arbabi, Nader Pestechian, Hadi Tavasol Khamseh, Hosein Hooshyar, and Mehdi Delavari
- Subjects
Echinococcus granulosus ,Camel ,Dog ,PCR ,Sequencing ,Medicine (General) ,R5-920 - Abstract
Background: Hydatidosis is one of the most important zoonotic parasitic disease worldwide caused by Echinococcus granulosus. Given the importance of parasite genotyping in disease control, this study was aimed to evaluate the genetic variation of the camel and dog isolates in Isfahan. Material and Methods: This cross-sectional study was done on infected livers and lungs of camels with hydatid cyst (n=51) during 2015-2016, collected from Zarinshahr and Najafabad industrial abattoir and 200 adult E. granulosus worms collected from stray dogs in different geographical regions of Isfahan for the molecular identification of mitochondrial genes (CO1, ND1). The DNA of protoscolices and adult worms were extracted, the PCR and sequencing were done using specific primers. Pairwise sequence alignment was used to identify the genetic similarity between the sequences and the registered ones in Gen bank using Clustalw2 software. Results: The PCR and sequencing of CO1 and ND1 genes on dog and camel samples showed different E. granulosus genotypes, including the common sheep strain (G1), bovid strain(G3) and camel strain (G6) in Isfahan region. The most frequent strains were the common sheep strain (G1). The genetic similarity between both ND1 and CO1 isolates taken from camel, dog and the isolates registered in Gene bank were 30.25-100% and 27.47-100% for camel and dog, respectively. Conclusion: Mitochondrial ND1 and CO1 genes are proper molecular markers for identifying the genetic variation of the camel and dog E. granulosus. The dominant strain of the worm in Isfahan, is the same as the other parts of the country, i.e., the G1 genotype (the most common sheep strain) belonged to the G1- G1 E. granulosus complex. more...
- Published
- 2017
19. Vitamin D Deficiency and Toxoplasma Infection
- Author
-
Zohre FAKHRIEH KASHAN, Saeede SHOJAEE, Hossein KESHAVARZ, Mohsen ARBABI, Mahdi DELAVARI, and Mahbobeh SALIMI
- Subjects
No Keywords### ,Public aspects of medicine ,RA1-1270 - Abstract
Vitamin D Deficiency and Toxoplasma Infection
- Published
- 2019
- Full Text
- View/download PDF
20. Prevalence of free living Amoeba in oral cavity of cancer patient, Kashan 2019 -2020
- Author
-
Sima Rasti, Tayebeh Taghipour, Mahdi Delavari, Hossein Hooshyar, Gholam Abbas Mousavi, and Mohsen Arbabi
- Subjects
free- living amoeba ,cancer patients ,prevalence ,oral cavity ,Medicine - Abstract
Introduction: Free –living Amoeba (FLA) are microorganisms that are abundant in the nature. Some genus of these protozoa are opportunistic and cause granulomatous encephalitis and lung infections in immunocompromised people such as cancer patients. Due to the little information about the status of infection of these parasites in the above patients, the present study was performed to determine the prevalence of FLA in cancer patients in Kashan hospitals. Materials and Methods: This cross-sectional study was performed on hundred oral cavity mucosal samples of cancer patients referred to Shahid Beheshti and Yathribi hospitals in Kashan during 2019 to 2020. The samples were cultured onto 1.5% Non-nutrient agar enriched with killed Escherichia coli and examined for the presence of FLA. The rate of contamination with free living amoebae and its relationship with demographic information of patients were analyzed by SPss. 16 Results: Overall, the prevalence of free-living amoeba in oral cavity of cancer patients was 89%. Also, 92.6% of breast cancer and 90.9% of bowel cancer patients were positive for FLA, respectively. The highest rate of FLA contamination was observed in women over the age of 66 years. In addition, the highest rate was observed in 90.9% of illiterate people and the lowest in 76.9% of academics, but the difference was not significant. Conclusion: The rate of free-living amoeba in oral cavity of cancer patients in Kashan were more than the study results of Iran and world. Therefore, health education about transmission and sanitation is recommended for prevention of infection. more...
- Published
- 2021
21. Urban Heat Island Monitoring and Impacts on Citizen’s General Health Status in Isfahan Metropolis: A Remote Sensing and Field Survey Approach
- Author
-
Mohsen Mirzaei, Jochem Verrelst, Mohsen Arbabi, Zohreh Shaklabadi, and Masoud Lotfizadeh
- Subjects
urban heat island ,land surface temperature ,split window algorithm ,general health questionnaire-28 ,Isfahan metropolis ,Science - Abstract
Urban heat islands (UHIs) are one of the urban management challenges, especially in metropolises, which can affect citizens’ health and well-being. This study used a combination of remote sensing techniques with field survey to investigate systematically the effects of UHI on citizens’ health in Isfahan metropolis, Iran. For this purpose, the land surface temperature (LST) over a three-year period was monitored by Landsat-8 satellite imagery based on the split window algorithm. Then, the areas where UHI and urban cold island (UCI) phenomena occurred were identified and a general health questionnaire-28 (GHQ-28) was applied to evaluate the health status of 800 citizens in terms of physical health, anxiety and sleep, social function, and depression in UHI and UCI treatments. The average LST during the study period was 45.5 ± 2.3 °C and results showed that the Zayandeh-Rood river and the surrounding greenery had an important role in regulating the ambient temperature and promoting the citizens’ health. Citizens living in the suburban areas were more exposed to the UHIs phenomena, and statistical analysis of the GHQ-28 results indicated that they showed severe significant (P < 0.05) responses in terms of non-physical health sub-scales (i.e., anxiety and sleep, social functioning, and depression). Therefore, it can be concluded that not all citizens in the Isfahan metropolis are in the same environmental conditions and city managers and planners should pay more attention to the citizens living in the UHIs. The most important proceedings in this area would be the creation and development of parks and green belts, as well as the allocation of health-medical facilities and citizen education. more...
- Published
- 2020
- Full Text
- View/download PDF
22. Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
- Author
-
Anita MOHAMMADIHA, Abdolhossein DALIMI, Mehdi MOHEBALI, Iraj SHARIFI, Mohammadreza MAHMOUDI, Asad MIRZAEI, Adel SPOTIN, Mahmoodreza BEHRAVAN, Mehdi KARIMI, Mohsen ARBABI, Shahram NEKOEIAN, Reza KALANTARI, and Behzad GHORBANZADEH more...
- Subjects
Phylogeny ,L. major ,L. tropica ,PCR-RFLP ,Human ,Iran ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been reported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. Methods: The smears were collected from lesions samples of 654 patients with CL, who attended local health centers in 12 provinces of Iran during 2013-2015. The smears were checked for the presence of amastigotes by light microscopy. DNA of 648 Leishmania isolates, amplified by targeting a partial sequence of ITS (18S rRNA–ITS1–5.8S rRNA–ITS2) gene. Twenty-five of all the amplicons were sequenced and analyzed with restriction fragment length polymorphism (RFLP) using the Taq1 enzyme. Results: All the smears were positive microscopically. The PCR-RFLP analysis revealed that 176 (27%) CL patients were infected with L. tropica and, 478 (73%) with L. major. The dominant species in all over Iran is L. major. The sequencing results of all CL patients and RFLP analysis confirmed each other. Based on our phylogenetic tree, 25 ITS DNA sequences were grouped into two clusters representing L. major and L. tropica species. Phylogenetic tree derived from the ITS sequences supports a clear divergence between L. major from the other species. Conclusion: Discrimination of Iranian Leishmania isolates using ITS gene gives us this opportunity to detect, identify, and construct the phylogenetic relationship of Iranian isolates. more...
- Published
- 2018
23. Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis
- Author
-
Zohreh FAKHRIEH-KASHAN, Mohsen ARBABI, Mahdi DELAVARI, Mahdi MOHEBALI, and Hossein HOOSHYAR
- Subjects
Trichomonas vaginalis ,Alcoholic extract ,Verbascum thapsus ,Ginger officinale ,In vitro ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: The protozoan Trichomonas vaginalis is a sexually transmitted disease (STD). Metronidazole is a chosen drug for the treatment. This study evaluated the anti trichomonal activity of alcoholic extracts of combination Verbascum thapsus and Ginger officinale. Methods: This experimental study was conducted in the Parasitology Laboratory, Kashan University of Medical Sciences, Kashan, Iran in 2015, on 23 women with suspected trichomoniasis referring to Kashan clinical centers. Medium TYI-S-33 was used for culture of three T. vaginalis isolates. Different concentrations (25, 50, 100, 200, 400, 800 µg/ml) of V. thapsus and G. officinale ethanol extract added to Trichomonas trophozoites in 48-well plates and metronidazole considered as positive control and the negative control was TYI-S33 containing Trichomonas trophozoites without any drug. In all of mentioned groups, trophozoites number counted 12, 24, 48 h after culture. Results were analyzed using ANOVA statistical test, to evaluate the toxicity of extract, measured by MTT assay. Induced apoptosis of T. vaginalis after treatment with different concentrations of extract was determined by Flow Cytometry. Results: IC50 of alcoholic extract of combination V. thapsus and G. officinale and metronidazole after 24h was 73.80 µg/ml and 0.0326 µg/ml, respectively. The toxicity percentage of 25-800 μg/ml concentrations of this combination were between 0.2-1.98. In different concentrations of extract (25,50,100,200 and 400 µg/ml) apoptosis percent after 48h was 18.97 to 77.19 and necrosis percent was calculated 1.35, 3.18, 3.10, 1.16 and 4.09, respectively. Conclusion: Alcoholic extract of combination V. thapsus and G. officinale induces programmed death in T. vaginalis. Due to no toxicity on macrophages, it can be examined in vivo studies. more...
- Published
- 2018
24. Developing a climate-based risk map of fascioliasis outbreaks in Iran
- Author
-
Mansour Halimi, Manuchehr Farajzadeh, Mahdi Delavari, and Mohsen Arbabi
- Subjects
Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Summary: The strong relationship between climate and fascioliasis outbreaks enables the development of climate-based models to estimate the potential risk of fascioliasis outbreaks. This work aims to develop a climate-based risk map of fascioliasis outbreaks in Iran using Ollerenshaw's fascioliasis risk index incorporating geographical information system (GIS). Using this index, a risk map of fascioliasis outbreaks for the entire country was developed. We determined that the country can be divided into 4 fascioliasis outbreak risk categories. Class 1, in which the Mt value is less than 100, includes more than 0.91 of the country's area. The climate in this class is not conducive to fascioliasis outbreaks in any month. Dryness and low temperature in the wet season (December to April) are the key barriers against fascioliasis outbreaks in this class. The risk map developed based on climatic factors indicated that only 0.03 of the country's area, including Gilan province in the northern region of Iran, is highly suitable to fascioliasis outbreaks during September to January. The Mt value is greater than 500 in this class. Heavy rainfall in the summer and fall, especially in Rasht, Astara and Bandar Anzaly (≥1000 mm/year), creates more suitable breeding places for snail intermediate hosts. Keywords: Ollerenshaw fascioliasis risk index, Climate, Gilan province, Iran more...
- Published
- 2015
- Full Text
- View/download PDF
25. An Examination of the Relationship between Visual Fatigue Symptoms with Flicker Value Variations in Video Display Terminal Users
- Author
-
Ehsanollah Habibi, Hassan Rajabi, and Mohsen Arbabi
- Subjects
Visual Fatigue, Flicker Value, VDT Operators ,Public aspects of medicine ,RA1-1270 - Abstract
In most sensitive occupations such as nuclear, military and chemical industries closed circuit systems and visual display terminals (VDTs) are used to carefully control and assess sensitive processes. Visual fatigue is one of the factors decreasing accuracy and concentration in operators causing faulty perception. This study aimed to find out a relationship between visual fatigue symptoms (VFS) of Flicker value variations in video display terminal (VDT) operators. This cross-sectional study, conducted in 2011, aimed to examine visual fatigue and determine the relationship between its symptoms and visual flicker value changes in 248 operators of VDTs in several occupations. The materials used in this study were a visual fatigue questionnaire of VDTs and a VFM-90.1 device. Visual fatigue was measured in two stages (prior to beginning to work and 60 min later). The data were analyzed by SPSS11.5, using descriptive statistics, paired t-test, simple and multiple linear regressions, correlation and recognition coefficients. Then regression equations of changes in flicker value depending on the changes in the main domains and the changes in final score before the questionnaire were obtained. Paired t-test indicated significant differences in the mean score of visual fatigue symptoms and the mean score of flicker value between the two stages, respectively (P ≤ 0.001). Simple and multiple regressions of flicker value variations, for the last visual fatigue changes in questionnaire score and the four main domains of the questionnaire were obtained R2 = 0.851 and R2 = 0.853, respectively. Correlation coefficient in the above tests indicated reverse and significant relationships among flicker value changes with changes in questionnaire score and visual fatigue symptoms. Diagnosing the first symptoms of visual fatigue could be an appropriate warning for VDTs operators in sensitive occupations to react suitably, in behavior and management, to control or treat visual fatigue and prevent errors efficiently. more...
- Published
- 2015
26. تأثیر عصارهی الکلی پیاز (cepa Allium)، لعل کوهستان (Oliveria decumbens Vent) و سنبلک (Muscari neglectum) بر رشد تریکوموناس واژینالیس در شرایط In vitro
- Author
-
Zohreh Fakhrieh-Kashan, Mohsen Arbabi, Mahdi Delavari, Hossein Hooshyar, and Mohsen Taghizadeh
- Subjects
Trichomonas vaginalis ,Allium cepa ,Oliveria decumbens Vent ,Muscari neglectum ,alcoholic extract ,Medicine ,Medicine (General) ,R5-920 - Abstract
مقدمه: تریکوموناس واژینالیس تك یاختهی تاژكدار بیماریزای دستگاه ادراری- تناسلی انسان است. هر ساله بیش از 170 میلیون نفر در دنیا به این بیماری انگلی مبتلا میگردند. با توجه به عوارض جانبی مترونیدازول به عنوان داروی رایج این بیماری، در این مطالعه اثر عصارهی الکلی پیاز (cepa Allium)، لعل کوهستان (Oliveria decumbens Vent) و کلاغک یا سنبلک (neglectum Muscari) بر رشد انگل تریکوموناس واژینالیس در شرایط آزمایشگاهی در سال 1392 بررسی گردید. روشها: این تحقیق با طراحی تجربی به صورت برونتنی روی ایزولههای تریکوموناس واژینالیس جدا شده از بیماران مراجعه کننده به 5 مرکز بهداشتی- درمانی کاشان در سال 1392 صورت گرفت. تأثیر غلظتهای مختلف از عصارههای الکلی پیاز، لعل کوهستان، کلاغک یا سنبلک با غلظتهای 5/12، 25، 50، 100، 200، 400، 800 و 1000 میکروگرم بر میلیلیتر بر رشد تروفوزوئیتهای تریکوموناس واژینالیس در 12، 24 و 48 ساعت پس از کشت سنجیده شد. همچنین تأثیر مترونیدازول به عنوان شاهد مثبت در غلظتهای 025/0، 050/0، 100/0، 200/0 و 400/0 میکروگرم بر میلیلیتر بر انگل مورد بررسی قرار گرفت. در همهی موارد، تأثیر عصارهها و دارو با شمارش و مقایسهی تعداد انگل زنده و مرده با استفاده از رنگآمیزی تریپان بلو محاسبه گردید. دادهها با استفاده از آزمون آنالیز آماری ANOVA (Analysis of variance) تجزیه و تحلیل شد. یافتهها: عصارههای الکلی پیاز، لعل کوهستان، کلاغک یا سنبلک بر رشد تروفوزوئیتهای تریکوموناس واژینالیس اثر ممانعت کنندگی دارد. 50IC (Half maximal inhibitory concentration) عصارههای الکلی گیاهان مورد بررسی و مترونیدازول 24 ساعت پس از کشت به ترتیب برای عصارهی الکلی لعل کوهستان 8/101، عصارهی الکلی پیاز 3/572، عصارهی الکلی سنبلک 4/329 و برای مترونیدازول 0326/0 میکروگرم بر میلیلیتر محاسبه گردید. نتیجهگیری: عصارههای الکلی پیاز، لعل کوهستان و کلاغک یا سنبلک اثر ضد تریکوموناسی دارند. خصوصیات ضد تریکوموناسی عصارهی الکلی لعل کوهستان، به مراتب بیشتر از عصارهی الکلی پیاز و سنبلک میباشد. تحقیقات بیشتر بر روی اجزای تشکیل دهندهی هر یک از عصارهها جهت مطالعات بعدی لازم میباشد. more...
- Published
- 2015
27. Molecular and Morphological Characterizations of Echinococcus granulosus from Human and Animal Isolates in Kashan, Markazi Province, Iran
- Author
-
Mohsen ARBABI, Majid PIRESTANI, Mahdi DELAVARI, Hossein HOOSHYAR, Amir ABDOLI, and Shahab SARVI
- Subjects
Echinococcus granulosus ,Molecular phylogenetics ,Morphological characteriza-tion ,PCR-RFLP ,Iran ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: One of the most important zoonotic helminths in the world is known as Echinococcus granulosus. Different strains of the E. granulosus have been described based on morphological and molecular characterizations, however, there is limited information regarding the characteristics of the phenotypes and genotypes of E. granulosus in Iran. Methods: The present study was prepared to evaluate the phenotypic and genotypic diversity of E. granulosus isolates collected from human, goat, sheep, and cattle based on 19 standard morphometric parameters and mitochondrial and nuclear genes (CO1, ND1, and ITS1) in Kashan, Markazi Province, Iran during 2013-2014. Results: The biometric analysis for the 19 characters revealed that the 19 morphometric values of cattle isolates were exceptionally higher than human, goat, and sheep isolates (P more...
- Published
- 2017
28. Genetic Variation of Giardia lamblia Isolates from Food-handlers in Kashan, Central Iran
- Author
-
Hossein HOOSHYAR, Shahrbanou GHAFARINASAB, Mohsen ARBABI, Mahdi DELAVARI, and Sima RASTI
- Subjects
Giardia ,Genetic variation ,Food-handlers ,PCR-RFLP ,Iran ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: Based on genotyping study of human isolates of Giardia lamblia; humans are mainly infected by two assemblages A and B. The present study was carried out to determine the sub-assemblages of G. lamblia isolated from food handlers referred to Kashan health centers, central Iran, 2015. Methods: In this cross-sectional study, 3653 stool samples collected from food-handlers that annually refer to health center for getting a health certification and examined microscopically for G. lamblia cyst. Totally, 44 isolates were selected from 47 Giardia positive samples. Cysts were partially purified by the sucrose density gradient method. After freeze-thaw cycles, genomic DNA was extracted using QIAamp Stool Mini kit. A single step PCR-RFLP method was used to amplify a 458bp fragment at the glutamate dehydrogenase (gdh) locus, restriction enzymes BspLI and RsaI were used for distinguish between genotypes A and B and their subgroups. Results: Of 44 isolates, 24(54.5%) were sub-assemblage AII, 9(20.5%) group B including 7(15.9%) BIII and 2(4.6%) BIV sub-assemblage and 11(25%) isolates showed a mixed pattern of AII and B. Sub-assemblage AI was not detected in this study. Conclusion: The higher rate of sub-assemblage AII demonstrated an anthroponotic origin of the infection so infected food-handlers could directly transmit this protozoan to consumers via contaminated food and water. For finding of pattern of transmission and distribution of Giardia assemblages and sub-assemblage, more studies in human and animal population in different regions are necessary. more...
- Published
- 2017
29. Prevalence of ruminants fascioliasis and their economic effects in Kashan, center of Iran
- Author
-
Hassan Khoramian, Mohsen Arbabi, Mahmood Mahami Osqoi, Mahdi Delavari, Hossein Hooshyar, and Mohammarreza Asgari
- Subjects
Economic losses ,Cattle ,Sheep ,Goat ,Prevalence ,Fascioliasis ,Arctic medicine. Tropical medicine ,RC955-962 ,Biology (General) ,QH301-705.5 - Abstract
Objective: To determine the prevalence and economic losses of Fasciola spp. isolates and costs incurred due to liver condemnation from ruminants in Kashan region, center of Iran from 2012 to 2013. Methods: In this cross-sectional study, a total number of 251 325 slaughtered sheep, goats and cattle (88 939 sheep, 151 924 goats and 10 462 cattle) were examined via necropsy for fasciolasis in industrial Kashan abattoirs located in center of Iran. The average weight was calculated as 1 000 g for sheep, 900 g for goat and 5 000 g for cattle in this study region. Based on market value, the average price of a kilo gram of normal liver at Kashan city during the study period was 8 USD. The amount of main nutrients and vitamins elimination in liver contaminated to fascioliasis was determined. Results: Overall 3.28% of the livers were found to be infected. For total number of sheep, goats and cattle slaughtered annually in region study, it was estimated that 7 505 livers were infected and total annual economic losses of fascioliasis of studied animals was 41 784 USD (based on market prices in study period). Of this, 23 360 USD, 30 240 USD and 15 400 USD were associated with the fascioliasis of sheep, goats and cattle, respectively. Conclusions: This study indicated that the Fasciola sp. clearly remains prevalent and causes considerable economic losses in study region in sheep, goats and cattle and presumably, other areas and hosts of Iran, providing baseline data for the future monitoring of this potentially important parasitic infection in the country. more...
- Published
- 2014
- Full Text
- View/download PDF
30. Identification of latent neosporosis in sheep in Tehran, Iran by polymerase chain reaction using primers specific for the Nc-5 gene
- Author
-
Mohsen Arbabi, Amir Abdoli, Abdolhossein Dalimi, and Majid Pirestani
- Subjects
Neospora caninum ,neosporosis ,sheep ,molecular characterization ,Iran ,Veterinary medicine ,SF600-1100 - Abstract
Little is known about latent infection and molecular characterisation of Neospora caninum in sheep (Ovis aries). In this study, 330 sheep samples (180 hearts and 150 brains) were analysed for N. caninum DNA by nested polymerase chain reaction (PCR) targeting the Nc-5 gene. Neospora caninum DNA was detected in 3.9% (13/330) of sheep samples. The parasite’s DNA was detected in 6.7% of heart samples (12/180) and 0.7% (1/150) of brain samples. No clinical signs were recorded from infected or uninfected animals. Sequencing of the genomic DNA revealed 96% – 99% similarity with each other and 95.15% – 100% similarity with N. caninum sequences deposited in GenBank. To our knowledge, this is the first report on the use of PCR to identify latent neosporosis in sheep in Iran. The results of this study have the potential to contribute to our understanding of the role of N. caninum-infected sheep in the epidemiology of neosporosis. more...
- Published
- 2016
- Full Text
- View/download PDF
31. Detection of Bendiocarb and Carbaryl Resistance Mechanisms among German Cockroach Blattella germanica (Blattaria: Blattellidae) Collected from Tabriz Hospitals, East Azerbaijan Province, Iran in 2013
- Author
-
Afshin Salehi, Hassan Vatandoost, Teimour Hazratian, Alireza Sanei-Dehkordi, Hossein Hooshyar, Mohsen Arbabi, Yaser Salim-Abadi, Reza Sharafati-Chaleshtori, Mohammad Amin Gorouhi, and Azim Paksa
- Subjects
German cockroach ,Resistance mechanism ,Carbaryl ,Bendiocarb ,Pathology ,RB1-214 - Abstract
Background: Insecticide resistance is one of the serious problems for German cockroach control program. This study was conducted to determine the bendiocarb and Carbaryl resistance mechanisms in German cockroaches using the piperonyl butoxide (PBO). Methods: Bioassay tests were conducted with 4 to 6 different concentrations of both insecticides with four replicates of 10 susceptible strain cockroaches per concentration to determine of discriminative concentration. After determining discriminative concentration, the result was compared to wild strain. The levels of susceptibility and resistance ratio (RR) and synergism ratio (SR) were calculated for each five wild strains. Moreover resistance mechanisms in wild strains were determined using PBO synergist in vivo. Results: Hospital strains showed different levels of resistance to bendiocarb and carbaryl compared to susceptible strain. The bendiocarb and carbaryl resistance ratios ranged from 2.11 to 7.97 and 1.67 to 2 at LD50 levels, respectively. The synergist PBO significantly enhanced the toxicity of bendiocarb and carbaryl to all strains with different degrees of synergist ratio, 1.31, 1.39, 3.61, 1.78, 1.62 and 2.1 fold for bendiocarb, 1.19, 1.18, 1.12. 1.29, 1.45 and 1.11- fold for carbaryl, suggesting monooxygenase involvement in bendiocarb and carbaryl resistance. Conclusion: The synergetic effect of PBO had the highest effect on bendiocarb and resistance level was significantly reduced, which indicates the important role of monoxidase enzyme in creating resistance to Bendiocarb. Piperonyl butoxide did not have a significant synergistic effect on carbaryl and did not significantly break the resistance. more...
- Published
- 2016
32. Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) in Petroleum Contaminated Soils
- Author
-
Mohsen Arbabi, Simin Nasseri, and Anyakora Chimezie
- Subjects
polycyclic aromatic hydrocarbons (pahs) ,bioremediation ,pah-degrading microorganisms ,pah contaminated soils ,Chemical engineering ,TP155-156 ,Chemistry ,QD1-999 - Abstract
Polycyclic aromatic hydrocarbons are a class of potentially hazardous chemicals of environmental and health concern. PAHs are one of the most prevalent groups of contaminants found in soil. Biodegradation of complex hydrocarbon usually requires the cooperation of more than single specie. In this research biotreatment of PAH (phenanthrene) was studied in a solid-phase reactor using indigenous bacteria isolated from two petroleum contaminated sites in Iran, (i.e., Tehran refinery site with clayey-sand soil composition and Bushehr oil zone with silty-sand soil composotion). Phenanthrene (C14H10) was made in three rates (100, 500, and 1000 mg/kg of soil) synthetically and was conducted with two bacterial mixed cultures for a period of 20 weeks. Highest removal (more than 85 %) of phenanthrene with rates of 100, 500 and 1000 mg/kg in clayey-sand soil with BMTRS (Bacterial Mix of Tehran Refinery Site) consortium was achieved within 3, 5 and 14 weeks, respectively as for silty-sand soil composition with BMBOZ (Bacterial Mix of Bushehr Oil Zone) consortium was achieved within 10, 17, and 19 weeks, respectively. Results for phenanthrene biotreatment in solid phase reactor revealed a significance relationship between concentration and type of microbial consortium with the removal efficiency of phenanthrene over the time (P value more...
- Published
- 2009
33. Optimization of SBR system for enhanced biological phosphorus and nitrogen removal
- Author
-
Mohsen Arbabi, Abbas Akbarzadeh, and Abbas Khodabakhshi
- Subjects
Denitrification ,enhanced biological phosphorus removal (EBPR) ,nitrification ,sequencing batch reactor (SBR) ,wastewater ,Environmental technology. Sanitary engineering ,TD1-1066 ,Environmental engineering ,TA170-171 ,Environmental sciences ,GE1-350 - Abstract
Aims: The aim of this study was the optimization of the SBR system for enhanced biological phosphorus and nitrogen removal. Materials And Methods: A lab-scale SBR consisting filling, pre-anoxic, anerobic, anoxic, aerobic, settling, decanting, and idle phases was proposed for simultaneous enhanced biological phosphorus and nitrogen removal (SEBPNR) from wastewater. Synthetic wastewater was used in this research. Glucose was used as a carbon source. The SBR was seeded with sludge from a local municipal wastewater treatment plant. Results: The results indicates that the lab-scale SBR was capable to remove soluble phosphorus (SP), SCOD, TCOD, and ammonia, with efficiencies of around 92%, 95%, 80%, and 85%, respectively. Optimized lab-scale SBR operational condition for SEBPNR consists of a fill (15 min), pre-anoxic (30 min), anerobic (90 min), 1st aerobic (210 min), 2nd anoxic (55 min), 2nd aerobic (10 min), settling (90 min), decant (10 min), and idle (10 min) phases. Conclusion: This study concludes that effective biological removal of phosphorus and nitrogen from wastewater using SBR occurs in sufficient HRT in the anaerobic and aerobic stages, adequate COD/TP ratios (up to 35). This system is suitable for high removal of P and N in both municipal and industrial wastewater. more...
- Published
- 2012
- Full Text
- View/download PDF
34. Development of an electrochemical fentanyl nanosensor based on MWCNT-HA/ Cu-H3BTC nanocomposite
- Author
-
Maryam Akbari, Maryam Saleh Mohammadnia, Masoumeh Ghalkhani, Mohammad Aghaei, Esmail Sohouli, Mehdi Rahimi-Nasrabadi, Mohsen Arbabi, Hamid Reza Banafshe, and Ali Sobhani-Nasab
- Subjects
General Chemical Engineering - Published
- 2022
- Full Text
- View/download PDF
35. In Vitro Anthelmintic Effect of Ferula assa-foetida Hydroalcoholic Extract Against Flukes of Fasciola hepatica and Dicrocoelium dendriticum
- Author
-
Mohsen Arbabi, Atefeh Haddad, Monireh Esmaeli, Hossein Hooshyar, and Mojtaba Sehat
- Subjects
General Pharmacology, Toxicology and Pharmaceutics - Abstract
Background: Dicrocoeliasis and fascioliasis are foodborne parasitic diseases of the biliary tract, resulting from Dicrocoelium dendriticum and Fasciola hepatica causing extensive financial losses and serious health problems in ruminants. Due to low-performance medications, drug delivery is a tremendous project to improve interventions available for these diseases. Objectives: This study aimed to determine the anthelmintic properties of Ferula assa-foetida extract against F. hepatica and D. dendriticum using in vitro assay. Methods: The effects of diverse concentrations of F. assa-foetida extract (400 - 1000 µg/mL) for 12-24 hours were examined for the treatment of D. dendriticum and F. hepatica. The anthelmintic efficacies were evaluated using scanning electron microscopy (SEM). The MTT assay was carried out to evaluate the cell viability of all cells in culture media. Results: The SEM images of treated worms by F. assa-foetida extract (200 µg/mL) confirmed excessive damage, which included an entire lack of sensory papillae and destruction of distinguished network structures and tegument vesicles. Variables of duration and concentration presented a considerable effect on both the mortality rate and the anthelmintic properties of F. assa-foetida; accordingly, as the time and concentration increased, the mortality rate became higher. Based on the MTT assay, the toxicity of F. assa-foetida at 800 µg/mL concentration was 8.7%. Therefore, it can be argued that F. assa-foetida had anthelmintic properties. Conclusions: This is the first study that evaluated the impact of F. assa-foetida on liver flukes of D. dendriticum and F. hepatica. Therefore, it paved the way for further studies on the control of those trematodes. It is recommended to document and look into the indigenous understanding of viable medicinal plants to provide evidence for their use. more...
- Published
- 2023
- Full Text
- View/download PDF
36. Combination of Nonionic Surfactants with Thermal-activated Persulfate for Simultaneous Removal of Phenanthrene and Pyrene from Alluvial Soil: A Kinetic Study
- Author
-
Gashtasb Mardani, Mehraban Sadeghi, Maryam Heidari, Mohsen Arbabi, Morteza Sedehi, and Zahra Emadi
- Subjects
Environmental remediation ,Health, Toxicology and Mutagenesis ,Soil Science ,Phenanthrene ,Persulfate ,Pollution ,Soil contamination ,Sodium persulfate ,chemistry.chemical_compound ,Soil structure ,chemistry ,Environmental Chemistry ,Pyrene ,Alluvium ,Nuclear chemistry - Abstract
Polycyclic aromatic hydrocarbons have adverse effects on the organisms, plants, and soil structure. Therefore, they should be removed by an appropriate and effective method. In this study, simultaneous removal of 500 mg/kg(soil) of phenanthrene and pyrene was investigated for the individual and combined application of Tween 80, Triton X-100, and sodium persulfate from the alluvial soil. The results revealed that the maximum removal efficiencies of phenanthrene (94.06 +/- 0.30%) and pyrene (90.07 +/- 0.19%) were observed in the presence of Triton X-100 + sodium persulfate (0.5 M) within 1 hour, respectively. Also, individual application of surfactants and sodium persulfate had efficiencies of less than 80%. It should be noted that the removal of phenanthrene and pyrene followed the first-order kinetic model. Economic evaluations of combined treatment demonstrated that the application of more proportion of Triton X-100 (10000 mg/L) + less proportion of sodium persulfate (0.3 M) is more cost-effective for the remediation of soil in the field study. Based on the results, the combined application of the washing process by Triton X-100 and persulfate oxidant can significantly improve the removal efficiencies and is a promising technique in the remediation of polycyclic aromatic hydrocarbons contaminated soil. more...
- Published
- 2021
- Full Text
- View/download PDF
37. Urban Heat Island Monitoring and Impacts on Citizen's General Health Status in Isfahan Metropolis: A Remote Sensing and Field Survey Approach
- Author
-
Mohsen Mirzaei, Zohreh Shaklabadi, Mohsen Arbabi, Jochem Verrelst, and Masoud Lotfizadeh
- Subjects
split window algorithm ,010504 meteorology & atmospheric sciences ,Land surface temperature ,Science ,general health questionnaire-28 ,land surface temperature ,urban heat island ,010501 environmental sciences ,Field survey ,01 natural sciences ,Geography ,13. Climate action ,Remote sensing (archaeology) ,Social function ,11. Sustainability ,General Earth and Planetary Sciences ,Isfahan metropolis ,General health ,Urban heat island ,Urban management ,0105 earth and related environmental sciences ,Social functioning ,Remote sensing - Abstract
Urban heat islands (UHIs) are one of the urban management challenges, especially in metropolises, which can affect citizens’ health and well-being. This study used a combination of remote sensing techniques with field survey to investigate systematically the effects of UHI on citizens’ health in Isfahan metropolis, Iran. For this purpose, the land surface temperature (LST) over a three-year period was monitored by Landsat-8 satellite imagery based on the split window algorithm. Then, the areas where UHI and urban cold island (UCI) phenomena occurred were identified and a general health questionnaire-28 (GHQ-28) was applied to evaluate the health status of 800 citizens in terms of physical health, anxiety and sleep, social function, and depression in UHI and UCI treatments. The average LST during the study period was 45.5 ± 2.3 °C and results showed that the Zayandeh-Rood river and the surrounding greenery had an important role in regulating the ambient temperature and promoting the citizens’ health. Citizens living in the suburban areas were more exposed to the UHIs phenomena, and statistical analysis of the GHQ-28 results indicated that they showed severe significant (P < 0.05) responses in terms of non-physical health sub-scales (i.e., anxiety and sleep, social functioning, and depression). Therefore, it can be concluded that not all citizens in the Isfahan metropolis are in the same environmental conditions and city managers and planners should pay more attention to the citizens living in the UHIs. The most important proceedings in this area would be the creation and development of parks and green belts, as well as the allocation of health-medical facilities and citizen education. more...
- Published
- 2022
38. Maintenance of liver fluke, Dicrocoelium dendriticum, outside the body of its native host
- Author
-
Atefeh Hadad, Hossein Hooshyar, Seyed Mostafa Hosseinpour Mashkani, Hossein Akbari, and Mohsen Arbabi
- Subjects
culture media ,survival rate ,Medicine (General) ,Host (biology) ,Dicrocoelium dendriticum ,in vitro ,Biology ,Liver fluke ,biology.organism_classification ,dicrocoelium dendriticum ,Andrology ,R5-920 ,Streptomycin ,roswell park memorial institute medium ,medicine ,Incubation ,Survival rate ,Median survival ,Fetal bovine serum ,medicine.drug - Abstract
Aims: In vitro cultivation of digenea would help the development of effective treatments and studies of the biology of the parasites. The goal of the present study was to optimize culture conditions for the maintenance liver fluke, Dicrocoelium dendriticum. Materials and Methods: Forty fresh D. dendriticum were collected from the sheep liver and washed three times with warm Roswell Park Memorial Institute (RPMI) 1640 Medium. The collected worms were transferred to 24-well Nunc-Immuno plates containing RPMI media supplemented with 50% of fetal bovine serum (FBS), 2% of sheep red blood cells (RBCs), 50 IU/ml of penicillin, and 50 mg/ml streptomycin. The mobility of the live/dead worms was observed by inverted microscope. The mean and median survival time was calculated by Kaplan–Meier model, and survival and hazard function graphs were also analyzed. Results: D. dendriticum was lived in vitro only for long periods of about 25 dyes. The 1st day of maintaining in culture media, one worm was dead and the number of dead worms was raised to 40 after 25 days of incubation. On the one hand, the mean survival time was 392 h with a confidence interval (CI) of 95% (384.8–400.03). On the other hand, the median survival time was 420 h with a CI of 95% (406.9–433.09). D. dendriticum was able to be alive in RPMI 1640 media for at least 25 days. Conclusion: RPMI 1640 supplemented with FBS, and RBCs can be used as short-term maintenance for the in vitro culture of D. dendriticum. The outcomes of the current study could be useful for many aspects of parasitological analysis. more...
- Published
- 2021
39. Genotypes Identification of echinococcus granulosus isolated from iranian dogs and camels using three polymerase Chain reaction-based methods of cox1 gene
- Author
-
Hossein Hooshyar, Nader Pestechian, Mohsen Arbabi, and Mahdi Delavari
- Subjects
Veterinary medicine ,Medicine (General) ,camel ,biology ,Sequence analysis ,sequencing ,biology.organism_classification ,Melting curve analysis ,DNA sequencing ,High Resolution Melt ,cox1 gene ,echinococcus granulosus ,high resolution melting ,law.invention ,polymerase chain reaction-restriction fragment length polymorphism ,R5-920 ,law ,Genotype ,parasitic diseases ,dog ,Restriction fragment length polymorphism ,Echinococcus granulosus ,Polymerase chain reaction - Abstract
Aims: Hydatidosis is an important zoonotic disease that is caused by a tiny tapeworm, namely Echinococcus granulosus. In this study, three polymerase chain reaction (PCR)-based methods, including, high resolution melting (HRM) analysis, DNA sequencing, and PCR-restriction fragment length polymorphism (RFLP) have been used for genotype the identification of E. granulosus isolates from dogs and camels in Zarinshahr and Najafabad, Isfahan province, Iran. Materials and Methods: A total of 200 adult worms of 40 dogs and 51 samples of camel hydatid cysts were examined. Molecular characterization of isolates was performed using HRM assay, sequencing of DNA, and digestion Rsa1 pattern coding for the mitochondrial cox1 gene. For analysis of the HRM melting curve, we used the Tm within the range of 77.50°C–79.23°C. Results: HRM analysis revealed that 72.5%, 15%, and 12.5% dog's genotypes and 41.17%, 21.56%, and 35.29% camel genotypes were G1, G3, and G6, respectively. PCR-RFLP analysis, spare parts 310 bp and 138 bp of cox1 that shows the G1 genotype in all of the isolates. Sequence analysis as well as HRM assay was confirmed genotypes of G1, G3, and G6 in camels and dogs. Based on three methods of the cox1 gene the dominant genotype was G1. Conclusion: The PCR-RFLP only identified the G1 genotype, whereas the HRM analysis, as well as DNA sequencing, were detected three genotypes G1, G3, G6, therefore, these two methods have enough accuracy for the determination of genotypes of E. granulosus. This information leads to a better understanding of the biological characteristics of E. granulosus genotypes in Iran and shows the camel as a source of human hydatidosis. more...
- Published
- 2021
40. Ambient air particulate matter (PM10) satellite monitoring and respiratory health effects assessment
- Author
-
Ali Ahmadi, Mohsen Arbabi, Mahssa Mohebbichamkhorami, Mohsen Mirzaei, Mohammad Sadegh Hassanvand, and Hamid Rouhi
- Subjects
Spirometry ,Acute exacerbation of chronic obstructive pulmonary disease ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Air pollution ,02 engineering and technology ,010501 environmental sciences ,medicine.disease_cause ,complex mixtures ,01 natural sciences ,Applied Microbiology and Biotechnology ,FEV1/FVC ratio ,020401 chemical engineering ,Environmental health ,Medicine ,0204 chemical engineering ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology ,medicine.diagnostic_test ,business.industry ,Incidence (epidemiology) ,Public Health, Environmental and Occupational Health ,Regression analysis ,respiratory system ,Particulates ,medicine.disease ,Pollution ,respiratory tract diseases ,business ,Body mass index - Abstract
Air particulate matter with an aerodynamic diameter of 10 µm or less (PM10) is one of the main causes of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). This study explored the relationship between PM10 by remote sensing and AECOPD in Chaharmahal-o-Bakhtiari province from 2014 to2018. PM10 concentrations were predicted and validated based on aerosol optical depth (AOD) from 161 images processed by MODIS sensor and ground air quality monitoring station data. Demographic information and spirometric indices of 2038 patients with AECOPD were collected and analyzed from the hospital during the studied periods. SPSS software was used to analyze the relationships between these two categories of information. There was a significant negative relationship between PM10 and FVC, FVC%, FEV1, FEV1%, FEF25-75, FEV1/FVC, PEF, and FEF25FVC indices (p more...
- Published
- 2020
- Full Text
- View/download PDF
41. Changes in immune profile affect disease progression in hepatocellular carcinoma
- Author
-
Farshid Fathi, Reza F Saidi, Hamid Reza Banafshe, Mohsen Arbabi, Majid Lotfinia, and Hossein Motedayyen
- Subjects
Pharmacology ,Carcinoma, Hepatocellular ,Th2 Cells ,Immunology ,Liver Neoplasms ,Disease Progression ,Leukocytes, Mononuclear ,Immunology and Allergy ,Humans ,Th17 Cells ,Th1 Cells ,T-Lymphocytes, Regulatory ,digestive system diseases - Abstract
Objective: Hepatocellular carcinoma (HCC) as a chronic liver condition is largely associated with immune responses. Previous studies have revealed that different subsets of lymphocytes play fundamental roles in controlling or improving the development and outcome of solid tumors like HCC. Hence, this study aimed to investigate whether immune system changes were related to disease development in HCC patients. Methods: Peripheral blood mononuclear cells were isolated from 30 HCC patients and 30 healthy volunteers using Ficoll density centrifugation. The isolated cells were stained with different primary antibodies and percentages of different immune cells were determined by flow cytometry. Results: HCC patients indicated significant reductions in the numbers of CD4+ cells, Tbet+IFNγ+cells, and GATA+IL-4+cells in peripheral blood in comparison with healthy individuals ( p < 0.05). There was no significant change in IL-17+RORγt+cells between patient and healthy groups. In contrast, Foxp3+CD127lowcell frequency was significantly higher in patients than healthy subjects ( p < 0.0001). The numbers of Th1, Th2, and Th17 cells were significantly lower in HCC patients than healthy control ( p < 0.0001), although the reduction in Th2 cell numbers was not statistically significant. On the contrary, Treg percentage showed a significant increase in patients compared to healthy subjects ( p < 0.0001). Other data revealed that Th1, Th2, and Th17 cell frequencies were significantly higher in healthy individuals than patients with different TNM stages of HCC, with the exception of Th2 in patients with stage II HCC ( p < 0.01–0.05). Treg percentage was significantly increased in patients with different TNM stages ( p < 0.0001). Among all CD4+ T cells, the frequency of Th2 cell was significantly associated with TNM stages of HCC ( p < 0.05). Conclusion: Our data provide further evidence to show that immune changes may participate in determining HCC progression and disease outcome. However, it should be mentioned that more investigations are needed to clarify our results and explain possible impacts of other immune cells on the pathogenesis of HCC. more...
- Published
- 2022
42. In Vitro Assessment of Anthelmintic Activities of AgO Nanoparticle in Comparison to Closantel Against Liver Fluke Dicrocoelium Dendriticum
- Author
-
Mohsen Arbabi, Atefeh Haddad, Seyed Mostafa HosseipourMashkani, and Hossein Hooshyar
- Abstract
Background: Dicrocoeliasis is a rare Food-Born parasitic disease of the grazing herbivores as well humans, caused by Dicrocoelium dendriticum making severe pathological changes of the liver and bile systems, and therapeutic options for treatment are limited. With the appearance of drug resistance in liver flukes, there is a need to focus on alternative approaches to control helminth parasites of veterinary importance. Because of low-performance medications; drug delivery poses a great challenge for better treatment of Dicrocoeliasis. The current study aims to determine the anthelmintic properties of silver oxide nanoparticles (AgO) as a new method in dicrocoeliasis treatment, in vitro assay.Methods: The impacts of various concentrations of AgO nanoparticles (50-200 µg/ml) for 12-24 hours were compared with the Closantel, as the chemical drug. The anthelmintic efficacy was measured by scanning electron microscopy (SEM) technique.Results: SEM images of treated worms by AgO (200 µg/ml) showed severe damage, including complete loss of sensory papillae and destruction of prominent network structures and tegument vesicles. The mortality rates how the anthelmintic properties of AgO were highly relied on time and concentration, as far as increasing the time and concentration cause increasing the mortality rate. According to the MTT assay, the toxicity of AgO, at concentrations, 800 µg/ml is 8.7 %. Conclusions: Hence, it could be concluded that AgO NPs performed anthelmintic properties effects. To the best of our knowledge, no previous reports have assessed the effect of AgO NP on liver fluke D.dendriticum. Therefore, the present study provides a basis for future research on the control of this common trematode. more...
- Published
- 2021
- Full Text
- View/download PDF
43. Quality of patient information in emergency department
- Author
-
Mohsen Arbabi, Mahtab Karami, Mehrdad Farzandipour, and Sakine Abbasi Moghadam
- Subjects
Hospital information system ,02 engineering and technology ,Iran ,Shahid ,Access to Information ,03 medical and health sciences ,0302 clinical medicine ,Patient satisfaction ,Health care ,0202 electrical engineering, electronic engineering, information engineering ,Electronic Health Records ,Humans ,Medicine ,030212 general & internal medicine ,Descriptive statistics ,business.industry ,Health Policy ,Emergency department ,medicine.disease ,General Business, Management and Accounting ,Checklist ,Data Accuracy ,Cross-Sectional Studies ,Data quality ,Hospital Information Systems ,020201 artificial intelligence & image processing ,Medical emergency ,Emergency Service, Hospital ,business - Abstract
Purpose Data comprise one of the key resources currently used in organizations. High-quality data are those that are appropriate for use by the customer. The quality of data is a key factor in determining the level of healthcare in hospitals, and its improvement leads to an improved quality of health and treatment and ultimately increases patient satisfaction. The purpose of this paper is to assess the quality of emergency patients’ information in a hospital information system. Design/methodology/approach This cross-sectional study was conducted on 385 randomly selected records of patients admitted to the emergency department of Shahid Beheshti Hospital in Kashan, Iran, in 2016. Data on five dimensions of quality, including accuracy, accessibility, timeliness, completeness and definition, were collected using a researcher-made checklist and were then analyzed in SPSS. The results are presented using descriptive statistics, such as frequency distribution and percentage. Findings The overall quality of emergency patients’ information in the hospital information system was 86 percent, and the dimensions of quality scored 87.7 percent for accuracy, 86.8 percent for completeness, 83.9 percent for timeliness, 79 percent for definition and 62.1 percent for accessibility. Originality/value Increasing the quality of patient information at emergency departments can lead to improvements in the timely diagnosis and management of diseases and patient and personnel satisfaction, and reduce hospital costs. more...
- Published
- 2019
- Full Text
- View/download PDF
44. Evaluation of Chemical Oxygen Demand and Color Removal from Leachate Using Coagulation/Flocculation Combined with Advanced Oxidation Process
- Author
-
Mohsen Arbabi, Sepideh Tousizadeh, Elaheh Tondro, Morteza Sedehi, and Arman Arbabi
- Subjects
General Medicine - Abstract
One of the basic practices in the field of waste management is the collection and treatment of leachate. Leachate from municipal waste due to high chemical oxygen demand (COD) and dark color is a potential pollutant of the environment, which causes a lot of problems in the absence of treatment and direct discharge to the environment. This study aimed to determine the efficiency of ultrasonic process in combination with coagulation and flocculation process using sodium ferrate in COD and color reduction.In this experimental study, all experiments were performed in batch conditions and with changing process variables such as pH and sonication time, and the effect of three parameters, including ultrasonic reaction time (15, 30, and 45 min), pH (2, 4, 5/5, and 7), and coagulant dosage (from 1 to 150 g/l) on the COD reduction and color removal, was evaluated. Coagulant concentration and then the removal efficiency of COD and color were analyzed by ANOVA using SPSS 18.The COD reduction and color removal were 87.05% and 88.6% in optimal condition (using 120 g/L of sodium ferrate at pH 5.5), with coagulation/flocculation, after ultrasound (15 min). Ultrasound (15 min) + sodium ferrate (without coagulation/flocculation) achieved 46.25% of COD reduction and 90.35% of color elimination, whereas the ultrasonic process alone allowed removing the COD and color in the leachate by less than 50%.The results indicate that C-F followed by ultrasonic can be used to efficiently reduce the organic matter and color from municipal waste leachate, and it would be an ideal option for leachate treatment. more...
- Published
- 2021
45. Ambient air particulate matter (PM
- Author
-
Mahssa, Mohebbichamkhorami, Mohsen, Arbabi, Mohsen, Mirzaei, Ali, Ahmadi, Mohammad Sadegh, Hassanvand, and Hamid, Rouhi
- Subjects
Research Article - Abstract
PURPOSE: Air particulate matter with an aerodynamic diameter of 10 µm or less (PM(10)) is one of the main causes of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). This study explored the relationship between PM(10) by remote sensing and AECOPD in Chaharmahal-o-Bakhtiari province from 2014 to2018. METHOD: PM(10) concentrations were predicted and validated based on aerosol optical depth (AOD) from 161 images processed by MODIS sensor and ground air quality monitoring station data. Demographic information and spirometric indices of 2038 patients with AECOPD were collected and analyzed from the hospital during the studied periods. SPSS software was used to analyze the relationships between these two categories of information. RESULTS: There was a significant negative relationship between PM10 and FVC, FVC%, FEV1, FEV1%, FEF25-75, FEV1/FVC, PEF, and FEF25FVC indices (p more...
- Published
- 2020
46. Epidemiology and economic loss of fasciolosis and dicrocoeliosis in Arak, Iran
- Author
-
Hossein Hooshyar, Elnaz Nezami, Mahdi Delavari, and Mohsen Arbabi
- Subjects
0301 basic medicine ,Veterinary medicine ,medicine.medical_specialty ,Dicrocoeliosis ,Iran ,SF1-1100 ,03 medical and health sciences ,Average price ,Epidemiology ,SF600-1100 ,medicine ,Fasciola hepatica ,Statistical analysis ,Fasciolosis ,General Veterinary ,biology ,Dicrocoelium dendriticum ,030108 mycology & parasitology ,Liver fluke ,biology.organism_classification ,medicine.disease ,Animal culture ,slaughtered animal ,dicrocoeliosis ,epidemiology ,fasciolosis ,economic loss ,Research Article - Abstract
Aim: Fasciolosis and dicrocoeliosis are important parasitic diseases worldwide, causing significant financial losses due to decrease in production and viscera condemnation in animals. We performed the current research to assess the epidemiology of these infections and determine their significance from an economic perspective in Arak, Iran. Materials and Methods: In total, we evaluated 118,463 sheep, 207,652 goats, and 43,675 cattle through necropsic analysis at the slaughterhouses. The average weight of sheep, goat, and cattle liver was 1000, 900, and 5000 g, respectively. The average price of liver in the market was 8 USD/kg. Moreover, the elimination of fundamental nutrients and vitamins was evaluated in infected livers. The prevalence of fasciolosis and dicrocoeliosis was determined. Analysis of variance test was applied for the statistical analysis, and the significance level was more...
- Published
- 2018
47. Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study
- Author
-
Sima Rasti, Mohsen Arbabi, Sara Napooni, Mahdi Delavari, and Hossein Hooshyar
- Subjects
0301 basic medicine ,Eosin ,biology ,030231 tropical medicine ,Viral tegument ,030108 mycology & parasitology ,biology.organism_classification ,medicine.disease ,Pathology and Forensic Medicine ,Staining ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,Colloidal gold ,parasitic diseases ,Ultrastructure ,medicine ,DNA fragmentation ,Cyst ,Anatomy ,Echinococcus granulosus - Abstract
Recurrence of hydatidosis may results from cyst fluid spillage during surgery. To inactivate the cyst content, different scolicidal agents have been introduced. Nevertheless, novel and more effective treatments are needed due to the associated complications. In the current study, we examined the scolicidal effects of gold nanoparticles on Echinococcus granulosus protoscoleces. Hydatid cysts of sheep liver were collected in this study. The cyst fluid containing protoscoleces was aspirated aseptically. The protoscoleces were exposed to gold nanoparticles at different concentrations (250, 500, 1000, 2000, and 4000 μg/mL). Eosin staining method was applied to determine the viability of protoscoleces at 5, 10, 20, 30, and 60 min. Moreover, cytotoxicity analysis, scanning electron microscopy (SEM) of ultrastructural changes, DNA fragmentation, were performed, and protoscoleces were studied following treatment. The significant scolicidal effects of gold nanoparticles were observed at all concentrations compared to the control group. A total of 4000 μg/mL of gold nanoparticles could destroy 76% of protoscoleces in 60 min. Cytotoxic effects of gold nanoparticle on J774 macrophage cell line, in minimum and maximum concentration (500 and 4000 μg/mL), were 1 and 11%, respectively. After treatment of protoscoleces with gold nanoparticles in different times and concentrations, considerable alteration in size, ultrastructure changes in tegument and shape of sucker, as well as DNA fragmentation of parasite were seen. Based on the results, gold nanoparticles could exert scolicidal effects on hydatid cyst protoscoleces; therefore, they can be applied in hydatid cyst treatment. more...
- Published
- 2018
- Full Text
- View/download PDF
48. Optimization of UV/H2O2/Fe3O4 process to remove aniline from aqueous solutions using central composite methodology
- Author
-
Sara Hemati, Mohsen Arbabi, and Babak Mayahi
- Subjects
Aqueous solution ,Design–Expert ,Central composite design ,Nanoparticle ,02 engineering and technology ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,01 natural sciences ,chemistry.chemical_compound ,Aniline ,chemistry ,Wastewater ,0210 nano-technology ,Benzene ,Hydrogen peroxide ,0105 earth and related environmental sciences ,Nuclear chemistry - Abstract
Aniline with a benzene ring in its structure is a toxic, carcinogenic and mutagenic compound that causes many diseases in humans. Various methods have been developed to remove this pollutant from the environment among which the advanced oxidation has been successful in obtaining higher aniline removal efficiency. In this study, a UV light and Fe3O4 nano catalyst were used to remove aniline. In this study, the efficiency of aniline removal was studied as a dependent variable and aniline, hydrogen peroxide and iron nano catalyst concentrations, time and pH were investigated as independent variables. The concentration of aniline was measured by spectrophotometer. The optimization of the process was determined using the response surface method design and the central composite design model. Design Expert software was used to analyze the data. The results showed that the aniline removal efficiency decreased with increase in nano-catalyst concentration, hydrogen peroxide concentration and time and decreased with increasing pH and aniline concentration. To achieve maximum efficiency (78.1%), the optimal values for pH; initial concentration; time; nanoparticle content and H2O2 content were 3.2; 101 mg L-1; 50 min; 0.45 g L-1 and 31.08 mmol L-1. The results showed that the photo-Fenton process has a desirable ability to remove aniline from aqueous solution at pilot scale. Therefore, it was suggested to study the efficiency of this process as one of the clean and environmentally friendly methods at full scale on real wastewater. more...
- Published
- 2018
- Full Text
- View/download PDF
49. Preparation of controlled porosity activated carbon from walnut shell for phenol adsorption
- Author
-
Mohsen Arbabi, Abbas Akbarzadeh, and Sara Hemati
- Subjects
Powdered activated carbon treatment ,Langmuir ,Sorbent ,Aqueous solution ,Chemistry ,Langmuir adsorption model ,symbols.namesake ,chemistry.chemical_compound ,Adsorption ,medicine ,symbols ,Phenol ,Activated carbon ,medicine.drug ,Nuclear chemistry - Abstract
Phenol and its derivatives constitute widespread water pollutants. They have been found to accelerate tumor formation, cancer, and mutation. In this paper, walnut shell residue has been used as a raw material in preparation of powder activated carbon by the method of chemical activation with zinc chloride for the adsorption of phenol from dilute aqueous solutions. The influence of the mass impregnation ratio (R = ZnCl2 /walnut shell) and physical activation by the CO2 stream on the physical and chemical properties of the prepared carbons was examined. The effects of main parameters such as contact time (t), initial phenol concentration (Co), and solution’s pH were studied on phenol adsorption. The maximum uptake of phenol at 25°C was 214 mg g–1 at pH near phenol pKa and Co = 1,000 mg L–1. All data were fitted well with Langmuir isotherm, but after CO2 modification, deviation from Langmuir shows that both physical and chemical adsorption occurred during adsorption. The kinetic data were fitted to different models such as pseudo-first-order, pseudo-second-order, and diffusion model. Pseudo-second-order model has been chosen as the best model. In overall, walnut shell shows excellent adaptive characteristics for the removal of phenol and appears as a very promising sorbent due to its high uptake capacity and to its low cost. © 2018 Desalination Publications more...
- Published
- 2018
- Full Text
- View/download PDF
50. Copper adsorption from aqueous solution by activated carbon of wax beans waste activated by magnetite nanoparticles
- Author
-
Sara Hemati, Mohsen Arbabi, Nazila Golshani, and Arman Arbabi
- Subjects
Wax ,Aqueous solution ,Langmuir adsorption model ,chemistry.chemical_element ,Environmental pollution ,Copper ,symbols.namesake ,Adsorption ,chemistry ,Wastewater ,visual_art ,visual_art.visual_art_medium ,medicine ,symbols ,Activated carbon ,medicine.drug ,Nuclear chemistry - Abstract
Copper, a heavy metal, causes environmental pollution through a variety of industrial processes in many countries. Adsorption is known as an effective and efficient way of removing heavy metals. The present study was conducted mainly to magnetize the carbon in wax bean waste with magnetite nanoparticles and use it as adsorbent to remove copper from aqueous solution. Carbon was obtained from wax beans waste and activated with magnetite nanoparticles. The characteristics of obtaining adsorbent were studied and analyzed by FE-SEM, BET, and FT-IR. In this study, the effect of pH, adsorbent dose, contact time and copper concentration on the efficiency of copper removal was investigated per full factorial design by the Design Expert Software. The concentration of copper was measured by atomic absorption spectrophotometer (Varian AA240). For statistical analysis of the experiment’s data, ANOVA and P-value were used. Copper initial concentration 100 mg/L, adsorbent dose 1 g/L, pH 7 and contact time 40 min were obtained as optimal conditions for copper removal. Investigation of the isotherms indicated that the experimental data of the process were correlated with Langmuir Model. The maximum capacity of copper adsorption of Langmuir Model was 49.75 mg/g. Findings indicated that at optimal conditions, the amount of copper adsorbed from synthetic wastewater and real wastewater was 99.73% and 63%, respectively. Therefore, this method is capable of removing copper effectively and could be used to remove this metal from industrial wastewaters. © 2018 Desalination Publications. All rights reserved. more...
- Published
- 2018
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.