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Detection of Bendiocarb and Carbaryl Resistance Mechanisms among German Cockroach Blattella germanica (Blattaria: Blattellidae) Collected from Tabriz Hospitals, East Azerbaijan Province, Iran in 2013

Authors :
Afshin Salehi
Hassan Vatandoost
Teimour Hazratian
Alireza Sanei-Dehkordi
Hossein Hooshyar
Mohsen Arbabi
Yaser Salim-Abadi
Reza Sharafati-Chaleshtori
Mohammad Amin Gorouhi
Azim Paksa
Source :
Journal of Arthropod-Borne Diseases, Vol 10, Iss 3 (2016)
Publication Year :
2016
Publisher :
Tehran University of Medical Sciences, 2016.

Abstract

Background: Insecticide resistance is one of the serious problems for German cockroach control program. This study was conducted to determine the bendiocarb and Carbaryl resistance mechanisms in German cockroaches using the piperonyl butoxide (PBO). Methods: Bioassay tests were conducted with 4 to 6 different concentrations of both insecticides with four replicates of 10 susceptible strain cockroaches per concentration to determine of discriminative concentration. After determining discriminative concentration, the result was compared to wild strain. The levels of susceptibility and resistance ratio (RR) and synergism ratio (SR) were calculated for each five wild strains. Moreover resistance mechanisms in wild strains were determined using PBO synergist in vivo. Results: Hospital strains showed different levels of resistance to bendiocarb and carbaryl compared to susceptible strain. The bendiocarb and carbaryl resistance ratios ranged from 2.11 to 7.97 and 1.67 to 2 at LD50 levels, respec­tively. The synergist PBO significantly enhanced the toxicity of bendiocarb and carbaryl to all strains with different degrees of synergist ratio, 1.31, 1.39, 3.61, 1.78, 1.62 and 2.1 fold for bendiocarb, 1.19, 1.18, 1.12. 1.29, 1.45 and 1.11- fold for carbaryl, suggesting monooxygenase involvement in bendiocarb and carbaryl resistance. Conclusion: The synergetic effect of PBO had the highest effect on bendiocarb and resistance level was significantly reduced, which indicates the important role of monoxidase enzyme in creating resistance to Bendiocarb. Piperonyl butoxide did not have a significant synergistic effect on carbaryl and did not significantly break the resistance.

Details

Language :
English
ISSN :
17357179 and 23222271
Volume :
10
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Journal of Arthropod-Borne Diseases
Publication Type :
Academic Journal
Accession number :
edsdoj.5135ce3153d471293efc2495093e86e
Document Type :
article