1. Long-chain fatty acids differentially alter lipogenesis in bovine and caprine mammary slices
- Author
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Mohamad B. Montazer Torbati, Laurence Bernard, Christine Leroux, Benoît Graulet, Yves Chilliard, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche génomique et physiologie de la lactation (GPL), and Institut National de la Recherche Agronomique (INRA)
- Subjects
activité lipogénique ,[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,medicine.medical_specialty ,CD36 ,Biology ,Tissue Culture Techniques ,03 medical and health sciences ,Rumen ,Mammary Glands, Animal ,Species Specificity ,In vivo ,Lactation ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Incubation ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,tranche mammaire ,Goats ,Lipogenesis ,Fatty Acids ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,longue chaîne d'acides gras ,040201 dairy & animal science ,Enzyme assay ,Milk ,Endocrinology ,medicine.anatomical_structure ,chemistry ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Cattle ,Female ,Animal Science and Zoology ,Food Science ,Polyunsaturated fatty acid - Abstract
Indirect comparisons from studies in vivo have suggested that caprine mammary tissue is less sensitive than bovine mammary tissue to the anti-lipogenic effect of long-chain fatty acids (LCFA), including specific rumen biohydrogenation (RBH) intermediates of polyunsaturated fatty acids (PUFA). Our objective was to investigate the effects on lipogenesis of 18-carbon LCFA differing in the degree of unsaturation and/or double bond conformation using cultured slices of bovine and caprine mammary tissues. Mammary tissues were collected from five multiparous Holstein × Normande cows and six multiparous Alpine goats in mid lactation. The expression of genes involved in milk component synthesis was measured in tissues collected at slaughter and after slice preparation:FASN, SCD1, CD36, SREBF1andPPARG1mRNA levels were higher in bovine than caprine samples, whereas the opposite was observed forCSN2mRNA levels. Bovine and caprine mammary slices were incubated for 20 h in a medium with BSA (control), cis-9-18 : 1, 18 : 2n-6, 18 : 3n-3, cis-9, trans-11-CLA, or trans-10, cis-12-CLA (the latter at 3 increasing concentrations: C1 (0·11 mm), C2 (0·16 mm), C3 (0·37 mm)). Lipogenesis was estimated by measuring the incorporation of14C-acetate into total lipid. Significant differences of individual LCFA (P
- Published
- 2012