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8. Molecular epidemiology of KPC-producing Klebsiella pneumoniae from invasive infections in Italy: Increasing diversity with predominance of the ST512 clade II sublineage

Author

Conte, Viola, Monaco, Monica, Giani, Tommaso, D'Ancona, Fortunato, Moro, Maria Luisa, Arena, Fabio, D'Andrea, Marco Maria, Rossolini, Gian Maria, Pantosti, Annalisa, Bianchi, E., Catania, M. R., Cavalcanti, P., De Nittis, R., Dusi, P. A., Grandesso, S., Gualdi, P., Imbriani, A., Pini, B., Vincenzi, C., Meledandri, M., Agrappi, C., Parisi, G., Pollini, S., Restelli, A., Rocchetti, A., Vailati, F., Aschbacher, R., Barbaro, A., Bona, R., Chirillo, M., Corradini, S., Cuccurullo, S., De Bernochi, A., Dodi, C., Giammanco, A., Mencacci, Antonella, Milano, F., Miragliotta, G., Mungiguerra, M., Pedna, M. F., Piana, Francesca, Porcheddu, G. M., Rossi, M. R., Santino, I., Sartor, A., Sartore, P., Conte V., Monaco M., Giani T., D'Ancona F., Moro M.L., Arena F., D'Andrea M.M., Rossolini G.M., Pantosti A., Bianchi E., Catania M.R., Cavalcanti P., De Nittis R., Dusi P.A., Grandesso S., Gualdi P., Imbriani A., Pini B., Vincenzi C., Meledandri M., Agrappi C., Parisi G., Pollini S., Restelli A., Rocchetti A., Vailati F., Aschbacher R., Barbaro A., Bona R., Chirillo M., Corradini S., Cuccurullo S., De Bernochi A., Dodi C., Giammanco A., Mencacci A., Milano F., Miragliotta G., Mungiguerra M., Pedna M.F., Piana F., Porcheddu G.M., Rossi M.R., Santino I., Sartor A., Sartore P., Conte, V, Monaco, M, Giani, T, D'Ancona, F, Moro, Ml, Arena, F, D'Andrea, Mm, Rossolini, Gm, Pantosti, A, AR-ISS Study Group on Carbapenemase-Producing K., pneumoniae: Bianchi E, Catania, Mr, Cavalcanti, P, De Nittis, R, Dusi, Pa, Grandesso, S, Gualdi, P, Imbriani, A, Pini, B, Vincenzi, C, Meledandri, Alessio, Agrappi, C, Parisi, G, Pollini, S, Restelli, Ugo, Rocchetti, Romualdo, Vailati, F, Aschbacher, R, Barbaro, A, Bona, R, Chirillo, M, Corradini, S, Cuccurullo, S, De Bernochi, A, Dodi, C, Giammanco, Anna, Mencacci, A, Milano, F, Miragliotta, Giuseppe, Mungiguerra, M, Pedna, Mf, Piana, Daniela, Porcheddu, Gm, Rossi, Mr, Santino, I, Sartor, A, and Sartore, P.

Subjects
0301 basic medicine, Microbiology (medical), gel, Klebsiella pneumoniae, genotype, 030106 microbiology, Population, cross-sectional studies, pulsed-field, multilocus sequence typing, electrophoresis, gel, pulsed-field, humans, italy, klebsiella infections, klebsiella pneumoniae, molecular epidemiology, serogroup, beta-lactamases, genetic variation, Settore BIO/19 - Microbiologia Generale, Cross-Sectional Studies, Electrophoresis, Gel, Pulsed-Field, Humans, Italy, Klebsiella Infections, Molecular Epidemiology, Multilocus Sequence Typing, Serogroup, beta-Lactamases, Genetic Variation, Genotype, Pharmacology, Pharmacology (medical), Infectious Diseases, law.invention, Microbiology, beta-Lactamase, 03 medical and health sciences, law, Pulsed-field gel electrophoresis, Typing, education, Polymerase chain reaction, Cross-Sectional Studie, education.field_of_study, biology, Molecular epidemiology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Settore MED/07 - Microbiologia e Microbiologia Clinica, bacterial infections and mycoses, Virology, electrophoresis, Multilocus sequence typing, Human, Klebsiella Infection
Abstract

Objectives The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanita surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP). Methods A total of 17 laboratories distributed across Italy collected all consecutive non-replicate CR-KP isolated from invasive infections during two different study periods (2011-12 and 2013). Carbapenemase genes were searched for by filter hybridization and confirmed by PCR and sequencing. KPC-producing K. pneumoniae (KPC-KP) were typed by PFGE and MLST. Capsular types were identified by wzi gene typing. Results Of the collected K. pneumoniae isolates (n = 461), the overall proportion of CR-KP was 36.2% (n = 167). The majority (97%) of the CR-KP were positive for the blaKPC gene. Among the KPC-KP population, nine different STs were detected with the majority of isolates (94%) belonging to the clonal group (CG) 258. A subpopulation that belonged to ST512 and showed an identical PFGE profile represented the majority (57%) of KPC-KP strains, with a countrywide distribution. Capsular characterization showed the predominance of the wzi154, cps-2 capsular type (88.8% of all CG258 strains). ST258 strains were associated with both cps-1 and cps-2 capsular types, while ST512 was associated with cps-2 only. Conclusions Although a trend to a polyclonal evolution of the Italian KPC-KP was noted, this study showed that the KPC-KP population remained largely oligoclonal with the wide diffusion of an ST512 lineage carrying cps-2 capsular type and producing the KPC-3 enzyme.

Published
2016

9. Incidence, Risk Factors and Outcome of Pre-engraftment Gram-Negative Bacteremia After Allogeneic and Autologous Hematopoietic Stem Cell Transplantation: An Italian Prospective Multicenter Survey

Author

Girmenia, C, Bertaina, A, Piciocchi, A, Perruccio, K, Algarotti, A, Busca, A, Cattaneo, C, Raiola, Am, Guidi, S, Iori, Ap, Candoni, A, Irrera, G, Milone, G, Marcacci, G, Scimè, R, Musso, M, Cudillo, L, Sica, S, Castagna, L, Corradini, P, Marchesi, F, Pastore, D, Alessandrino, Ep, Annaloro, C, Ciceri, F, Santarone, S, Nassi, L, Farina, C, Viscoli, C, Rossolini, Gm, Bonifazi, F, Rambaldi, A, Capria, S, Mastronuzzi, A, Pagliara, D, Bernaschi, P, Amico, L, Carotti, A, Mencacci, A, Bruno, B, Costa, C, Passi, A, Ravizzola, G, Angelucci, E, Marchese, A, Pecile, P, Ventura, G, Fanin, R, Scarparo, C, Barbaro, A, Leotta, S, Marchese, Ae, Becchimanzi, C, Donnarumma, D, Tringali, S, Baldi, Mt, Scalone, R, Picardi, A, Arcese, W, Fontana, C, Giammarco, S, Spanu, T, Crocchiolo, R, Casari, E, Mussetti, A, Conte, E, Ensoli, F, Miragliotta, G, Marone, P, Arghittu, M, Greco, R, Forcina, A, Chichero, P, Di Bartolomeo, P, Fazii, P, Kroumova, V, Decembrino, N, Zecca, M, Pisapia, G, Palazzo, G, Lanino, E, Faraci, M, Castagnola, E, Bandettini, R, Pastano, R, Sammassimo, S, Passerini, R, Stefani, Pm, Gherlinzoni, F, Rigoli, R, Prezioso, L, Cambò, B, Calderaro, A, Carella, Am, Cascavilla, N, Labonia, Mt, Celeghini, I, Mordini, N, Piana, F, Vacca, A, Sanna, M, Podda, G, Corsetti, Mt, Rocchetti, A, Cilloni, D, De Gobbi, M, Bianco, O, Fagioli, F, Carraro, F, De Intinis, G, Severino, A, Proia, A, Parisi, G, Vallisa, D, Confalonieri, M, Russo, D, Malagola, M, Galieni, P, Falcioni, S, Travaglini, V, Raimondi, R, Borghero, C, Pavan, G, Prete, A, Belotti, T, Ambretti, S, Imola, M, Mianulli, Am, Pedna, Mf, Cesaro, S, Lo Cascio, G, Ferrari, A, Piedimonte, M, Santino, I, Calandrelli, M, Olivieri, A, Orecchioni, F, Mirabile, M, Centurioni, R, Gironacci, L, Caravelli, D, Gallo, S, De Filippi, M, Cupelli, L, Dentamaro, T, Falco, S, Eugenio, Os, Marotta, S, Risitano, A, Lula, D, Musto, P, Pietrantuono, G, Traficante, A, Cerchiara, E, Tirindelli, Mc, Dicuonzo, G, Chierichini, A, Anaclerico, B, and Placanica, P.

Published
2017

10. Exploiting information sharing, stock management and capacity oversizing in the management of lumpy demand.

Author

Miragliotta, G. and Staudacher, A. Portioli

Subjects
SUPPLY & demand, INVENTORY control, INDUSTRIAL capacity, PRODUCTION management (Manufacturing), MARKETING, WORKING capital
Abstract

The aim of this paper is to present a new approach to the management of uncertain lumpy demand. After providing a comprehensive review of the related literature, a new model is introduced, with the objective of improving the firm's ability to cope with lumpy orders. Its innovative principle relies on the exploitation of the information collected by the Sales Department during the negotiation process; such information helps to minimize the impact of the lumpy orders, thus reducing the need for oversized production capacity and/or working capital. An analytical model is presented to solve the trade-off between anticipating the production as soon as the order negotiation starts (thus risking production with no final order) and waiting until negotiation ends (thus risking a low service level due to the shorter time available to respond to the lumpy order): the right balance is found by keeping the right amount of stocks and of overcapacity. The model described turns out to be simple and easy to implement, since its informative needs consist of data usually available within the company, although often not exploited. [ABSTRACT FROM AUTHOR]

Published
2004
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11. Adopting the Internet of Things technologies in agriculture: an analysis of the benefits in the organic wine industry

Author

Mangiaracina, R., Miragliotta, G., Perego, A., Salvadori, G., and ANGELA TUMINO

Subjects
Risk, Internet of things, Organizational Behavior and Human Resource Management, Organic, Strategy and Management1409 Tourism, Leisure and Hospitality Management, Smart agriculture, Management Science and Operations Research, Benefits, Industrial and Manufacturing Engineering, Wine industry, Management of Technology and Innovation, Reliability and Quality, Business and International Management, Strategy and Management1409 Tourism, Leisure and Hospitality Management, Safety, Risk, Reliability and Quality, Waste Management and Disposal, Safety
Published
2016

13. Clostridium difficile. A review on an emerging infection.

Author

Del Prete, R., Ronga, L., Addati, G., Magrone, R., Abbasciano, A., Decimo, M., and Miragliotta, G.

Subjects
CLOSTRIDIOIDES difficile, PSEUDOMEMBRANOUS enterocolitis, DIARRHEA, MEDICAL care, TOXICITY testing
Abstract

Clostridium difficile causes antibiotic-associated diarrhoea and pseudomembranous colitis. The main virulence factors of C. difficile are the toxins A (TcdA) and B (TcdB). A third toxin, binary toxin (CDT), which pathogenetic role had been remained largely overlooked until few years ago, nowadays have been detected in 5%-23% of strains. C. difficile has spread around world. Clostridium difficile infection (CDI) is one of the most common health-care associated infections and a significant cause of morbidity and mortality among older adult hospitalized patients. Diagnosis of CDI is often difficult and has a substantial impact on the management of patients with disease. It is usually based on a clinical history of recent antimicrobial usage and diarrhoea in combination with laboratory tests. Although the conventional methods are crucial for the diagnosis and the subsequent treatment of CDI, a timely laboratory diagnosis is essential for the detection of toxigenic strains providing either to an effective and immediately treatment or to the prevention of further disease transmission. In this review we provide general recommendations for testing of samples collected from patients with suspected CDI. [ABSTRACT FROM AUTHOR]

Published
2019
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17. Microbiologia medica. 7. Edizione

Author

DI FRANCESCO, P, Angiolella, L, Azzi, A, Bonina, L, Caputo, A, Cavallo, R, Cenci, E, D'Ameliio, S, De Giuli Morghen, C, DI CAVE, D, Di Luca, D, Di Nocera, P, Donnarumma, G, Gismondo, M, Miragliotta, G, Palamara, A, Perito, S, Radaelli, A, and Zanotto, C

Subjects
Settore MED/07 - Microbiologia e Microbiologia Clinica
Published
2013

18. Microbiologia Medica

Author

Di Francesco, P, Angiolella, L, Azzi, A, Bonina, L, Caputo, A, Cavallo, R, Cenci, E, D'Amelio, S, De Giuli Morghen, C, Di Cave, D, Di Luca, D, Di Nocera, P, Donnarumma, G, Gismondo, M, Miragliotta, G, Palamara, A, Perito, S, Radaelli, A, Zanotto, C, Casalinuovo, I, Costa, C, Donadel, G, Gabrielli, E, Pericolini, E, and Rotola, A

Subjects
Settore MED/04 - Patologia Generale, Settore MED/07 - Microbiologia e Microbiologia Clinica
Published
2013

19. Sherris - Microbiologia Medica

Author

Beninati, C., Bistoni, F., Bonaventura, G., Ceccherini Nellu, L., Cenci, E., Cocuzza, C., Cuffini, A. M., D'Agostini, C., Di Cave, D., Di Cuonzo, G., DI LUCA, Dario, Favalli, C., Galdiero, M., Gariglio, M., Giammanco, A., Gralli, S., Landolfo, S., Massida, O., Mastino, A., Mastromarino, P., Mattina, R., Miragliotta, G., Oliva, B., Pagani, L., Palù, G., Parolin, C., Perito, S., Piperno, M., Santino, I., Scalia, G., Sessa, R., Sinibaldi, P., Talenti, D., and Teti, G.

Subjects
Microbiologia Medica
Published
2013

22. Microbiologia Medica

Author

Beninati, Concetta, Bistoni, F, Bonaventura, G, Checcherini Nelli, L, Cenci, E, Cocuzza, C, Cuffini, Am, D'Agostini, C, Di Cave, D, Di Cuonzo, G, Di LUCa, D, Favalli, C, Galdiero, M, Gariglio, M, Giammanco, A, Grelli, S, Landolfo, S, Massidda, O, Mastino, Antonio, Mastromarino, P, Mattina, R, Miragliotta, G, Oliva, B, Pagani, L, Palù, G, Parolin, C, Perito, S, Piperino, M, Santino, I, Scalia, G, Sessa, R, Sinibaldi Vallebona, P, Valenti, D, and Teti, Giuseppe

Subjects
microbiologia
Published
2012

25. Medical Microbiology

Author

Bennati, C., Bistoni, F., Bonaventura, G., CECCHERINI-NELLI, Luca, Cenci, E., Cocuzza, C., Cuffini, A. M., DI Cave, D., Di Cuonzo, G., Di Luca, D., Favalli, C., Galdiero, M., Gariglio, M., Giammanco, A., Grelli, S., Landolfo, S., Massidda, O., Mastino, A., Mastromarino, P., Mattina, R., Miragliotta, G., Oliva, B., Pagani, L., Palù, G., Parolin, C., Perito, S., Santino, J., Scalia, G., Sessa, R., Sinimbaldi Vallebona, P., Valenti, D., and Teti, G.

Subjects
Microbiologia, Microbiologia Medica
Published
2011

26. Medical Microbiology

Author

Cavallo, R., CECCHERINI-NELLI, Luca, D’Agostini, C., Debbia, Ea, Dicuonzo, G., Ferrante, P., Galdiero, M., Mancini, C., Miragliotta, G., Modiano, D., Pagani, L., and Sessa, R.

Subjects
Medical, Microbiology
Published
2011

27. Membro Comitato scientifico XXIII Congresso Nazionale di Agopuntura dell'AIAM e Membro della Commissione interuniversitaria di ricerca in agopuntura (XVIII incontro), 6 Marzo, Roma (relazione S. Ricci: Introduzione scientifica ai lavori)Registro del Ministero della Salute cod. identif. 1763 – 10005933 (medici) e 1763 – 10005934 (fisioterapisti)

Author

Osborn, J., Balacco Gabrieli, C., Camaioni, D., Ippoliti, F., Arbarello, P., Nicoletti, W. E., Ricci, Serafino, Miragliotta, G., Pontieri, G. M., Spitali, R., Petti, F., and Liguori, A.

Published
2010

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