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3C 84 (NGC 1275) is the radio source at the centre of the Perseus cluster and exhibits a bright radio jet. We observed the source with the Global Millimeter VLBI Array (GMVA) between 2008 and 2015, with a typical angular resolution of ∼50 μas. The observations revealed a consistent double nuclear structure separated by ∼770 gravitational radii assuming a black hole mass of 3.2 × 108 M⊙. The region is likely too broad and bright to be the true jet base anchored in the accretion disc or black hole ergosphere. A cone and parabola were fit to the stacked (time averaged) image of the nuclear region. The data did not strongly prefer either fit, but combined with a jet/counter-jet ratio analysis, an upper limit on the viewing angle to the inner jet region of ≤35° was found. This provides evidence for a variation of the viewing angle along the jet (and therefore a bent jet) within ∼0.5 pc of the jet launching region. In the case of a conical jet, the apex is located ∼2400 gravitational radii upstream of the bright nuclear region and up to ∼600 gravitational radii upstream in the parabolic case. We found a possible correlation between the brightness temperature and relative position angle of the double nuclear components, which may indicate rotation within the jet. © 2021 The Author(s)., This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2021R1A6A3A01086420). JAH was supported by Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT, South Korea (2018H1D3A1A02032824) and the research grant (2021R1C1C1009973). S-SL was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MIST) (2020R1A2C2009003). J-YK is supported for this research by the International Max-Planck Research School (IMPRS) for Astronomy and Astrophysics at the University of Bonn and Cologne. This research was supported by an appointment to the NASA Postdoctoral Program at the Goddard Space Flight Center, administered by Universities Space Research Association through a contract with NASA. RL acknowledges the support of the Spanish Ministerio de Economía y Competitividad (grant PID2019-108995GB-C21), the Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (grant P18-FR-1769), and the Consejo Superior de Investigaciones Científicas (grant 2019AEP112). GFP is supported for this research by the International Max-Planck Research School (IMPRS) for Astronomy and Astrophysics at the University of Bonn and Cologne. ST and MK acknowledge support via NRF grant 2019R1F1A1059721. This research has made use of data obtained with the Global Millimeter VLBI Array (GMVA), which consists of telescopes operated by the MPIfR, IRAM, Onsala, Metsahovi, Yebes, the Korean VLBI Network, the Green Bank Observatory, and the Very Long Baseline Array (VLBA). The VLBA is an instrument of the NRAO, which is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc. The data were correlated at the correlator of the Max Planck Institute for Radioastronomy (MPIfR) in Bonn, Germany. This work made use of the Swinburne University of Technology software correlator, developed as part of the Australian Major National Research Facilities Programme and operated under licence., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709.

Atomically sharp heterojunctions in lateral two-dimensional heterostructures can provide the narrowest one-dimensional functionalities driven by unusual interfacial electronic states. For instance, the highly controlled growth of patchworks of graphene and hexagonal boron nitride (h-BN) would be a potential platform to explore unknown electronic, thermal, spin or optoelectronic property. However, to date, the possible emergence of physical properties and functionalities monitored by the interfaces between metallic graphene and insulating h-BN remains largely unexplored. Here, we demonstrate a blue emitting atomic-resolved heterojunction between graphene and h-BN. Such emission is tentatively attributed to localized energy states formed at the disordered boundaries of h-BN and graphene. The weak blue emission at the heterojunctions in simple in-plane heterostructures of h-BN and graphene can be enhanced by increasing the density of the interface in graphene quantum dots array embedded in the h-BN monolayer. This work suggests that the narrowest, atomically resolved heterojunctions of in-plane two-dimensional heterostructures provides a future playground for optoelectronics., This work was supported by the research funds (NRF-2017R1E1A1A01074493 and NRF-2019R1A4A1027934) and the grant (CASE-2013M3A6A5073173) from the centre for Advanced Soft Electronics under the Global Frontier Research Program through the National Research Foundation by the Ministry of Science and ICT, Korea. H.S.S. and K.S. thank the A3 foresight program for their collaboration. Y.S., Y.-C.L. and K.S. acknowledge JSPS KAKENHI Grant Numbers JP19K05223, JP18K14119 and JP16H06333, respectively. S.R. acknowledges the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No. 881603 (Graphene Flagship). ICN2 is funded by the CERCA Programme/Generalitat de Catalunya, and is supported by the Severo Ochoa program from Spanish MINECO (Grant No. SEV-2017-0706). J.E.B.-V. acknowledges funding from PAIP Facultad de Química, UNAM (Grant No. 5000-9173).

Atmospheric blockings and their impacts on wintertime surface air temperature in East Asia are investigated by using three blocking indices. Blockings in two specifc regions that indirectly or directly modulate East Asian climate variability are analyzed: Ural (UR) blocking over East Asia’s northwest and Okhotsk (OK) blocking over East Asia’s northeast. Both lead to cold anomalies in East Asia but through slightly diferent mechanisms. In the typical case of UR blocking, cold air associated with a mid-level trough develops to the east of the quasi-stationary blocking and slowly moves southeastward as the Siberian high intensifes. Four days after the blocking onset, East Asia experiences signifcant cooling due to the temperature advection associated with anomalous temperature gradient and adiabatic cooling. During the typical OK blocking event, a preexisting cold anomaly over northern Eurasia is pushed southeastward by the westward expansion of the blocking. The anomalous meridional fow due to blocking-induced dipolar circulation over the North Pacifc further reinforces the cold advection over East Asia, with a minor contribution of vertical processes. In both types of blocking, the cold anomaly persists even in the decaying phase, due to an ofset between the temperature advection and the diabatic heating., The 28th IUGG General Assembly (IUGG2023) (Berlin 2023)

Type 4 sphingosine-1-phosphate receptor, which mediates the activation of the Nod-like receptor (NLR) family pyrin domain containing 3 inflammasome, emerges as a new therapeutic target for nonalcoholic steatohepatitis. We developed a selective functional antagonist for type 4 sphingosine-1-phosphate receptor (SLB736), which protected mice against nonalcoholic steatohepatitis and fibrosis, Supported by the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology, Korea, grants 2017R1E1A1A01073206 (K.-U.L.), 2020R1A2B5B02098524 (E.H.K.), 2019R1C1C1003567 (J.-Y.Y.), and 2021R1A2C1005520 (J.W.C.), the Korea Drug Development Fund funded by the Ministry of Science and ICT, Ministry of Trade, Industry, and Energy, and Ministry of Health and Welfare grant KDDF-201406-03 (K.-U.L.), and Asan Institute for Life Sciences, Korea, grant 2020IP0016-1 (E.H.K.). This work also was supported by the Mid-Career Researcher Programs grant NRF-2019R1A2C2009905 (S.K.) of the National Research Foundation of Korea, funded by the Government of Korea (MSIP); and by grants PID2019-111669RB-I00 and PID2020-115055RB-I00 from Plan Nacional de Investigación y Desarrollo, Spain, by the Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Spain; by grant P50AA011999 from the Southern California Research Center for Alcoholic Liver and Pancreatic Diseases and Cirrhosis funded by National Institute of Alcohol Abuse and Alcoholism; by Agency for Administration of University and Research Grants of the Generalitat de Cataluña SGR-2017-1112 and the “ER stress-mitochondrial cholesterol axis in obesity-associated insulin resistance and comorbidities” Ayudas Fundación Banco Bilbao Vizcaya Argentaria a Equipos de Investigación Científica 2017, the Red Nacional 2018-102799-T de Enfermedades Metabólicas y Cáncer, and by project 201916/31 Contribution of mitochondrial oxysterol and bile acid metabolism to liver carcinogenesis 2019 by Fundació Marato Televisión 3. None of the funders had a role in the study design, collection, analysis, or interpretation of data.

To date, little is known about the past evolutionary trajectories of rare and endangered orchids native to mainland China, Japan, and Korea (the CJK region). In this study, we focus on two endangered orchids, Cypripedium japonicum (present in the three countries) and C. formosanum (endemic to Taiwan), to understand the divergence/speciation models that would have been operating in this group, including genetic diversity, geographic structure, and colonization pathways across the region. Using a combination of five cpDNA regions, we reconstructed phylogenetic trees and investigated the genetic diversity/structure of 20 populations. Ecological niche modeling was used to gain insight into the paleodistribution and dispersal corridors at the Last Glacial Maximum and to survey climatic niche differences. Populations from mainland China + Korea, Japan, and Taiwan formed three distinct monophyletic lineages and were placed into separate genetic clusters, agreeing with geographic barriers and species boundaries. Populations of C. japonicum in mainland China harbored the highest diversity, suggesting the presence of multiple glacial refugia. The Korean populations would have originated from either western/central or eastern China, probably using a dispersal corridor across the East China Sea shelf. The divergence of C. formosanum is proposed under an allopatric speciation model, also highly influenced by a climate niche shift. In the context of previous studies, a deep divergence in cpDNA sequences between Chinese + Korean and Japanese populations of C. japonicum may be taken as an example of the speciation events of the CJK flora since the late Neogene that have led to its current species richness., This study was supported by the Biodiversity Survey, Observation and Assessment Program of the Ministry of Ecology and Environment of China to HZT and by Basic Science Program through the National Research Foundation of Korea (NRF-2017R1A2B4012215) to MGC, and funded by the Ministry of Science and ICT of the Republic of Korea (NRF-2020R1I1A3074635) to MYC., INTRODUCTION MATERIALS AND METHODS Study species Population sampling DNA extraction cpDNA-PCR optimum primer selection cpDNA sequence alignment and assembly Haplotype distribution, phylogenetic analyses, and genetic diversity Genetic differentiation and structure Mismatch distribution analysis, neutrality detection, and demographic history ENM and population connectivity Niche comparisons in E-space RESULTS Haplotype distribution and phylogeny Genetic diversity Genetic differentiation and structure Mismatch distribution analysis, neutrality detection, and demographic history ENM and population connectivity Niche comparisons in E-space DISCUSSION Deep genetic and climatic divergence of Cypripedium sect. Flabellinervia in the CJK region: taxonomic considerations Haplotype and nucleotide diversity in Cypripedium sect. Flabellinervia: inference of glacial refugia and demographic history Origin of Korean populations of Cypripedium japonicum Origin of Cypripedium formosanum CONCLUSIONS AUTHOR CONTRIBUTIONS ACKNOWLEDGEMENTS Appendix 1: The cpDNA sequence information of Cypripedium sect. Flabellinervia deposited in the GenBank database

Several papers deal with a conservation genetics gap in which plant conservation and restoration managers or practitioners do not soundly integrate population genetics information into conservation management. Authors concerned about this issue point out that practitioners perceive genetic research results to be impractical or unnecessary in the short term due to time and financial constraints. In addition, researchers often fail to translate research findings into comprehensive, jargon-free recommendations effectively. If possible, conservation-related or conservation-oriented articles should be easily written to bridge the research¿implementation gap. Finally, based on a previously published prioritization framework for conservation genetics scenarios, we introduce four simple genetic categories by exemplifying each case. We hope that conservation practitioners could employ these suggested guidelines for the prioritization of population- and species-level management., This research was supported by the Basic Science Research Program through the National Research Foundation (NRF), funded by the Ministry of Science and ICT of the Republic of Korea (NRF- 2020R1I1A3074635 to M.Y.C. and NRF-2017R1A2B4012215 to M.G.C.), and was partly carried out as part of the study on population characteristics and conservation management strategies of rare plants in the Korea National Arboretum (KNA1-2-37, 20-4), who supported M.G.C. in 2021. K.M. was supported by the National Natural Science Foundation of China (grant no. U20A2080, 31622015) while J.L.-P. is funded by the Spanish Ministerio de Ciencia e Innovación (PID2020-119163GB-I00), 1. Introduction 2. Bridging the Gap between Conservation Geneticists and Conservation Practitioners: Some Examples 3. Expected Scenarios According to Levels of Neutral Genetic Parameters: A Simplified Proposal 4. A Prioritization Framework for Conservation Strategies Based on Neutral Genetic Diversity Scenarios 5. Conclusions Author Contributions Funding Acknowledgments Conflicts of Interest References

This work was supported by Samsung Electronics (Samsung-SKKU Graphene/2D Center) and the research fund (NRF-2017R1E1A1A01074493 and NRF-2019R1A4A1027934) through the National Research Foundation by the Ministry of Science and ICT, Korea. M.C. acknowledges support from Leverhulme Trust Research grant RPG-2019-227.

Proper carbon flux distribution between cell growth and production of a target compound is important for biochemical production because improper flux reallocation inhibits cell growth, thus adversely affecting production yield. Here, using a synthetic biosensor to couple production of a specific metabolite with cell growth, we spontaneously evolve cells under the selective condition toward the acquisition of genotypes that optimally reallocate cellular resources. Using 3-hydroxypropionic acid (3-HP) production from glycerol in Escherichia coli as a model system, we determine that mutations in the conserved regions of proteins involved in global transcriptional regulation alter the expression of several genes associated with central carbon metabolism. These changes rewire central carbon flux toward the 3-HP production pathway, increasing 3-HP yield and reducing acetate accumulation by alleviating overflow metabolism. Our study provides a perspective on adaptive laboratory evolution (ALE) using synthetic biosensors, thereby supporting future efforts in metabolic pathway optimization., This research was supported by the C1 Gas Refinery Program (NRF-2018M3D3A1A01055754 and NRF-2015M3D3A1A01064882), the Global Research Laboratory Program (NRF-2016K1A1A2912829), and the Bio & Medical Technology Development Program (NRF-2018M3A9H3020459) through the National Research Foundation of Korea (NRF of Korea), funded by the Ministry of Science and ICT (MSIT). S.W.S. is partially supported by the Creative-Pioneering Researchers Program through Seoul National University (SNU).

[EN] Reviews that summarize the genetic diversity of plant species in relation to their life history and ecological traits show that forest trees have more genetic diversity at population and species levels than annuals or herbaceous perennials. In addition, among-population genetic differentiation is significantly lower in trees than in most herbaceous perennials and annuals. Possible reasons for these differences between trees and herbaceous perennials and annuals have not been discussed critically. Several traits, such as high rates of outcrossing, long-distance pollen and seed dispersal, large effective population sizes (N), arborescent stature, low population density, longevity, overlapping generations, and occurrence in late successional communities, may make trees less sensitive to genetic bottlenecks and more resistant to habitat fragmentation or climate change. We recommend that guidelines for genetic conservation strategies be designed differently for tree species versus other types of plant species. Because most tree species fit an LH scenario (low [L] genetic differentiation and high [H] genetic diversity), tree seeds could be sourced from a few populations distributed across the species’ range. For the in situ conservation of trees, translocation is a viable option to increase N. In contrast, rare herbaceous understory species are frequently HL (high differentiation and low diversity) species. Under the HL scenario, seeds should be taken from many populations with high genetic diversity. In situ conservation efforts for herbaceous plants should focus on protecting habitats because the typically small populations of these species are vulnerable to the loss of genetic diversity. The robust allozyme genetic diversity databases could be used to develop conservation strategies for species lacking genetic information. As a case study of reforestation with several tree species in denuded areas on the Korean Peninsula, we recommend the selection of local genotypes as suitable sources to prevent adverse effects and to insure the successful restoration in the long term., [ES] Las revisiones que resumen la diversidad genética de las plantas en relación con sus características ecológicas y biológicas muestran que los árboles forestales tienen más diversidad genética a nivel de población y de especie que las plantas anuales o las perennes herbáceas. Sumado a esto, la diferenciación genética entre poblaciones es significativamente más baja en los árboles que en la mayoría de las perennes herbáceas y las anuales. Hasta la fecha no se han discutido críticamente las posibles explicaciones de estas diferencias entre los árboles y las perennes herbáceas y las plantas anuales. Varias características, como las tasas altas de alogamia, la dispersión a gran distancia de polen y semillas, el gran tamaño de la población efectiva (Ne), la estatura arbórea, la baja densidad poblacional, la longevidad, el solapamiento de generaciones y la presencia dentro de comunidades sucesionales tardías, pueden generar en los árboles una menor sensibilidad a los cuellos de botella genéticos y una mayor resistencia a la fragmentación del hábitat o al cambio climático. Recomendamos que las directrices para las estrategias de conservación genética estén diseñadas de manera diferente para las especies arbóreas que para otro tipo de plantas. Ya que la mayoría de las especies arbóreas encajan dentro de un escenario LH (baja [L] diferenciación genética y alta [H] diversidad genética), las semillas de los árboles podrían tomarse de unas cuantas poblaciones dispersas a lo largo del área de distribución de la especie. Por lo anterior, para la conservación in situ de los árboles, la translocación es una opción viable para incrementar la Ne. Al contrario de esta situación, las especies herbáceas raras del sotobosque con frecuencia son especies HL (alta diferenciación y baja diversidad). En el escenario HL, las semillas deberían ser recolectadas de muchas poblaciones con diversidad genética alta y los esfuerzos de conservación in situ para las plantas herbáceas deberían enfocarse en la protección del hábitat ya que las poblaciones típicamente pequeñas de estas especies son vulnerables a la pérdida de la diversidad genética. Las robustas bases de datos de diversidad genética aloenzimática podrían usarse para desarrollar estrategias de conservación para las especies que carecen de información genética. Como caso de estudio de reforestación con varias especies arbóreas en áreas deforestadas de la Península de Corea, recomendamos la selección de genotipos locales como fuente adecuada para prevenir los efectos adversos y para asegurar la restauración exitosa a largo plazo., This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT of the Republic of Korea (NRF-2013R1A1A2063524 to M.Y.C. and NRF-2017R1A2B4012215 to M.G.C.), Introduction Higher Genetic Diversity of Trees than Herbaceous Perennials or Annuals Strategies for Conserving Genetic Diversity in Trees and Herbaceous Species Case Study of Restoration of a Denuded Area Conclusions Acknowledgments

Background/Aims: Myelodysplastic syndrome (MDS) is caused by genetic and epigenetic alteration of hematopoietic precursors and immune dysregulation. Approximately 20% of patients with MDS develop an autoimmune disease (AID). Here, we investigated whether particular genetic mutations are associated with AID in patients with MDS. Methods: Eighty-eight genetic mutations associated with myeloid malignancy were sequenced in 73 MDS patients. The association between these mutations and AID was then analyzed. Results: The median age of the 73 MDS patients was 70 years (interquartile range, 56 to 75), and 49 (67.1%) were male. AID was observed in 16 of 73 patients (21.9%). Mutations were detected in 57 (78.1%) patients. The percentage (68.8% vs. 80.7%, p = 0.32) and the mean number of mutations (1.8 ± 1.6 vs. 2.2 ± 1.8, p = 0.34) in MDS patients with or without AID were similar. However, the ten-eleven translocation-2 (TET2) mutation rate was significantly higher in patients with AID than in those without (31.3% vs. 5.3%, respectively; p = 0.001). All TET2 mutations were variants of strong clinical significance. Conclusions: Mutation of TET2 in patients with MDS may be associated with increased risk of developing AID., We thank Professor Eun Bong Lee for critical review of the manuscript. This research was supported by Basic Science Research Program through the Nation-al Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (NRF-2017R1A2A1A17069780).

The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article., Funding is acknowledged from the European Research Council (ERC Advanced Grant No. 787510-4DBIOSERS to L.M.L.-M., ERC Advanced Grant No. 789104-eNANO to F.J.G.A., ERC Starting Grant No. 259432-MULTIBIOPHOT to J.K., ERC Consolidator Grant No. 772108-DarkSERS); the Department of Education of the Basque Government (Grant No. IT1164-19 to J.A.); the Spanish MINECO (CTQ2017-88648-R to R.A.-P., MAT2016-77809-R to I.P.-S. and J.P.-J.); the EPSRC (EP/P034063/1 to S.B., EP/L027151/1 to J.B., EP/L014165/1 to D.G. and K.F.); IDUN-Danish National Research Foundation (DNRF122) and Villum Fonden (Grant No. 9301) to A.B.; the National Research Foundation of Korea (Grant No. 2019R1A2C3004375 to J.B.); the German Science Foundation, DFG (SFB 1278 Polytarget (Project B4) to V.D., Grant No. SCHL 594/13-1 to S.S., Germany’s Excellence Strategy (EXC 2089/1-390776260) to S.M.); the Federal Ministry of Education and Research, Germany (BMBF) (Grant InfectoGnostics 13GW0096F to D.C.-M. and J.P.); DARPA-16-35-INTERCEPT-FP-018 to L.F.; the UK BBSRC (Grant No. BB/L014823/1 to R.G.); the Department of Science and Technology (DST Nanomission Project SR/NM/NS-23/2016 to K.G.T.); the U.S. National Science Foundation (Grant No. CHE-1707859 to A.J.H., Center for Sustainable Nanotechnology CHE-1503408 (Centers for Chemical Innovation Program) to C.L.H., Center for Chemical Innovation Chemistry at the Space-Time Limit (CaSTL) CHE-1414466 to G.C.S. and R.P.V.D., Grant No. CHE-1807269 to K.A.W.); the Knut and Alice Wallenberg Foundation to M.K.; the Office of Naval Research (Grant No. N00014-18-1-2876 to N.A.K.); Royal Society of New Zealand Te Apa̅rangi to E.L.R. and B.A.; Singapore Ministry of Education, Tier 1 (RG11/18) to X.Y.L.; the Photoexcitonix Project in Hokkaido Univ., Japan, to K.M.; BioNano Health-Guard Research Center funded by the Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project (Grant No. H-GUARD_2013M3A6B2078947) to J.-M.N.; NSFC of P. R. China (Grant No. 21705015 to Y.O., Grant No. 21633005 to B.R.); National Key R&D Program (2017YFA0206902) to C.X. This work was coordinated under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency—Grant No. MDM-2017-0720.

Autophagy is a highly conserved catabolic process in eukaryotic cells by which waste cellular components are recycled to maintain growth in both favorable and stress conditions. Autophagy has been linked to lipid metabolism in microalgae; however, the mechanism underlying this interaction remains unclear. In this study, transgenic Chlamydomonas reinhardtii cells that stably express the red fluorescent protein (mCherry) tagged-ATG8 as an autophagy marker were established. By using this tool, we were able to follow the autophagy process in live microalgal cells under various conditions. Live-cell and transmission electron microscopy (TEM) imaging revealed physical contacts between lipid droplets and autophagic structures during the early stage of nitrogen starvation, while fusion of these two organelles was observed in prolonged nutritional deficiency, suggesting that an autophagy-related pathway might be involved in lipid droplet turnover in this alga. Our results thus shed light on the interplay between autophagy and lipid metabolism in C. reinhardtii, and this autophagy marker would be a valuable asset for further investigations on autophagic processes in microalgae., This research was funded by a grant from Marine Biotechnology Program (20150184) funded by Ministry of Oceans and Fisheries, Korea; by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Science and ICT(ABC-2015M3A6A2065697); and by grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (www.kribb.re.kr).

Ribozymes are functional RNA molecules that can catalyze biochemical reactions. Since the discovery of the first catalytic RNA, various functional ribozymes (e.g., self-cleaving ribozymes, splicing ribozymes, RNase P, etc.) have been uncovered, and their structures and mechanisms have been identified. Ribozymes have the advantage of possessing features of “RNA” molecules; hence, they are highly applicable for manipulating various biological systems. To fully employ ribozymes in a broad range of biological applications in synthetic biology, a variety of ribozymes have been developed and engineered. Here, we summarize the main features of ribozymes and the methods used for engineering their functions. We also describe the past and recent efforts towards exploiting ribozymes for effective and novel applications in synthetic biology. Based on studies on their significance in biological applications till date, ribozymes are expected to advance technologies in artificial biological systems., This work was supported by the National Research Foundation of Korea (NRF) grant (NRF-2017R1C1B3012050) and the Global Research Laboratory Program (NRF-2016K1A1A2912829) funded by the Korea government (Ministry of Science and ICT). Also, this research was supported by Basic Science Research Program funded by the Ministry of Education (2018R1A6A3A11045727).

Many immune diseases occur at different rates among people with schizophrenia compared to the general population. Here, we evaluated whether this phenomenon might be explained by shared genetic risk factors. We used data from large genome-wide association studies to compare the genetic architecture of schizophrenia to 19 immune diseases. First, we evaluated the association with schizophrenia of 581 variants previously reported to be associated with immune diseases at genome-wide significance. We identified five variants with potentially pleiotropic effects. While colocalization analyses were inconclusive, functional characterization of these variants provided the strongest evidence for a model in which genetic variation at rs1734907 modulates risk of schizophrenia and Crohn’s disease via altered methylation and expression of EPHB4—a gene whose protein product guides the migration of neuronal axons in the brain and the migration of lymphocytes towards infected cells in the immune system. Next, we investigated genome-wide sharing of common variants between schizophrenia and immune diseases using cross-trait LD score regression. Of the 11 immune diseases with available genome-wide summary statistics, we observed genetic correlation between six immune diseases and schizophrenia: inflammatory bowel disease (rg = 0.12 ± 0.03, P = 2.49 × 10−4), Crohn’s disease (rg = 0.097 ± 0.06, P = 3.27 × 10−3), ulcerative colitis (rg = 0.11 ± 0.04, P = 4.05 × 10–3), primary biliary cirrhosis (rg = 0.13 ± 0.05, P = 3.98 × 10−3), psoriasis (rg = 0.18 ± 0.07, P = 7.78 × 10–3) and systemic lupus erythematosus (rg = 0.13 ± 0.05, P = 3.76 × 10–3). With the exception of ulcerative colitis, the degree and direction of these genetic correlations were consistent with the expected phenotypic correlation based on epidemiological data. Our findings suggest shared genetic risk factors contribute to the epidemiological association of certain immune diseases and schizophrenia., This research was supported in part by a number of funding sources. This research uses resources provided by the Genetic Association Information Network (GAIN), obtained from the database of Genotypes and Phenotypes (dbGaP) found at http://www.ncbi.nlm.nih.gov/gap through dbGaP accession number phs000021.v3.p2; samples and associated phenotype data for this study were provided by the Molecular Genetics of Schizophrenia Collaboration (PI: Pablo V. Gejman, Evanston Northwestern Healthcare and Northwestern University, Evanston, IL, USA). Fulbright Canada, the Weston Foundation, and Brain Canada through the Canada Brain Research Fund—a public-private partnership established by the Government of Canada (to J.G.P.); the National Research Foundation of Korea (NRF) [grant 2016R1C1B2013126 to B.H.] and the Bio & Medical Technology Development Program of the NRF [grant 2017M3A9B6061852 to B.H.] funded by the Korean government, Ministry of Science and ICT; the Finnish Cultural Foundation and Academy of Finland [grant 309643 to H.M.O.]; the Spanish Ministry of Economy and Competitiveness and P12-BIO-1395 from Consejería de Innovación, Ciencia y Tecnología, Junta de Andalucía (Spain) [grant SAF2015-66761-P to J.M.]; the US National Institutes of Health (NIH) [grants R01AR045584, R01AR056292, X01HG007484 and P30AR057212 to Y.J., S.A.S. and R.S.]; the US NIH [grants N01AR02251 and R01AR05528 to M.D.M.]; the US NIH [grants 1R01AR063759, 1R01AR062886, 1UH2AR067677-01 and U19AI111224-01 to S.R.] and Doris Duke Charitable Foundation [grant 2013097 to S.R.]. Funding for the GAIN schizophrenia sample was provided by the US NIH [grants R01 MH67257, R01 MH59588, R01 MH59571, R01 MH59565, R01 MH59587, R01 MH60870, R01 MH59566, R01 MH59586, R01 MH61675, R01 MH60879, R01 MH81800, U01 MH46276, U01 MH46289, U01 MH46318, U01 MH79469 and U01 MH79470] and the genotyping of samples was provided through GAIN. The funding sources did not influence the study design, data analysis or writing of this manuscript.

Algae undergo a complete metabolic transformation under stress by arresting cell growth, inducing autophagy and hyperaccumulating biofuel precursors such as triacylglycerols and starch. However, the regulatory mechanisms behind this stress-induced transformation are still unclear. Here, we use biochemical, mutational, and “omics” approaches to demonstrate that PI3K signaling mediates the homeostasis of energy molecules and influences carbon metabolism in algae. In Chlamydomonas reinhardtii, the inhibition and knockdown (KD) of algal class III PI3K led to significantly decreased cell growth, altered cell morphology, and higher lipid and starch contents. Lipid profiling of wild-type and PI3K KD lines showed significantly reduced membrane lipid breakdown under nitrogen starvation (-N) in the KD. RNA-seq and network analyses showed that under -N conditions, the KD line carried out lipogenesis rather than lipid hydrolysis by initiating de novo fatty acid biosynthesis, which was supported by tricarboxylic acid cycle down-regulation and via acetyl-CoA synthesis from glycolysis. Remarkably, autophagic responses did not have primacy over inositide signaling in algae, unlike in mammals and vascular plants. The mutant displayed a fundamental shift in intracellular energy flux, analogous to that in tumor cells. The high free fatty acid levels and reduced mitochondrial ATP generation led to decreased cell viability. These results indicate that the PI3K signal transduction pathway is the metabolic gatekeeper restraining biofuel yields, thus maintaining fitness and viability under stress in algae. This study demonstrates the existence of homeostasis between starch and lipid synthesis controlled by lipid signaling in algae and expands our understanding of such processes, with biotechnological and evolutionary implications., This work was supported by a grant from the host institution through the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (www.kribb.re.kr), the Advanced Biomass R&D Center of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning (2015M3A6A2065697), and a grant from the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries (20150184).

[EN] Heuristically known at least since the first half of XIX century, the self-healing capacity of cement-based materials has been receiving keen attention from the civil engineering community worldwide in the last decade. As a matter of fact, stimulating and/or engineering the aforementioned functionality via tailored addition and technologies, in order to make it more reliable in an engineering perspective, has been regarded as a viable pathway to enhance the durability of reinforced concrete structures and contribute to increase their service life. Research activities have provided enlightening contributions to understanding the mechanisms of crack self-sealing and healing and have led to the blooming of a number of self-healing stimulating and engineering technologies, whose effectiveness has been soundly proved in the laboratory and, in a few cases, also scaled up to field applications, with ongoing performance monitoring. Nonetheless, the large variety of methodologies employed to assess the effectiveness of the developed self-healing technologies makes it necessary to provide a unified, if not standardized, framework for the validation and comparative evaluation of the same self-healing technologies as above. This is also instrumental to pave the way towards a consistent incorporation of self-healing concepts into structural design and life cycles analysis codified approaches, which can only promote the diffusion of feasible and reliable self-healing technologies into the construction market. In this framework the Working Group 2 of the COST Action CA 15202 "Self-healing as preventive repair of concrete structures - SARCOS" has undertaken the ambitious task reported in this paper. As a matter of fact this state of the art provides a comprehensive and critical review of the experimental methods and techniques, which have been employed to characterize and quantify the self-sealing and/or self-healing capacity of cement-based materials, as well as the effectiveness of the different self-sealing and/or self healing engineering techniques, together with the methods for the analysis of the chemical composition and intrinsic nature of the self-healing products. The review will also address the correlation, which can be established between crack closure and the recovery of physical/mechanical properties, as measured by means of the different reviewed tests. (C) 2018 Elsevier Ltd. All rights reserved., The authors acknowledge the support from EU COST Action CA 15202 "SARCOS" (http://www.sarcos.eng.cam.ac.uk). E. Cuenca and L. Ferrara acknowledge the financial support of the PoliMi International Fellowships 2015 (PIF). T. Van Mullem and N. De Belie acknowledge the grant (17SCIP-B103706-03) from the Construction Technology Research Program funded by Ministry of Land, Infrastructure and Transport of Korean Government.

Empirical evidence reveals that contagion processes often occur with competition of simple and complex contagion, meaning that while some agents follow simple contagion, others follow complex contagion. Simple contagion refers to spreading processes induced by a single exposure to a contagious entity while complex contagion demands multiple exposures for transmission. Inspired by this observation, we propose a model of contagion dynamics with a transmission probability that initiates a process of complex contagion. With this probability nodes subject to simple contagion get adopted and trigger a process of complex contagion. We obtain a phase diagram in the parameter space of the transmission probability and the fraction of nodes subject to complex contagion. Our contagion model exhibits a rich variety of phase transitions such as continuous, discontinuous, and hybrid phase transitions, criticality, tricriticality, and double transitions. In particular, we find a double phase transition showing a continuous transition and a following discontinuous transition in the density of adopted nodes with respect to the transmission probability. We show that the double transition occurs with an intermediate phase in which nodes following simple contagion become adopted but nodes with complex contagion remain susceptible., MSM and BM acknowledge financial support from the Agencia Estatal de Investigaci on (AEI, Spain) and Fondo Europeo de Desarrollo Regional under project ESOTECOS FIS2015-63628-C2-2-R (MINECO/AEI/FEDER, UE). This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2018R1C1B5044202).

A series of isostructural cobalt hydroxychloride salts with the formula Co(OH)Cl were fabricated by using a precipitation method from aqueous solutions of CoCl and NaOH with different mixing ratios. The crystal structure was resolved from neutron powder diffraction data, including the location of H atoms of the hydroxide groups. The structure (space group R3m) consists of an open network of edge-sharing CoO and CoOCl octahedra, forming intercrossed 1D channels where the hydrogen atoms reside. Unusually high values of the magnetic entropy change were observed for all samples, probably due to the geometrically frustrated magnetic structure; for example, the magnetic entropy change was 8.3 J/kg K for the sample with Co/Cl > 2.5 at 10 K in a magnetic field change of 20 kOe., This work was supported by the Converging Research Center Program through the Ministry of Science, ICT and Future Planning, Korea (201505411), and the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education (2015063125). J. A. A. acknowledges the Spanish Ministry of Economy and Competitivity by funding the Project MAT2013‐41099‐R.

8 pags., 6 figs., Positioning probe molecules at electromagnetic hot spots with nanometer precision is required to achieve highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS) analysis. In this article, molecular positioning at plasmonic nanogaps is reported using a high aspect ratio (HAR) plasmonic nanopillar array with a controlled surface energy. A large-area HAR plasmonic nanopillar array is generated using a nanolithography-free simple process involving Ar plasma treatment applied to a smooth polymer surface and the subsequent evaporation of metal onto the polymer nanopillars. The surface energy can be precisely controlled through the selective removal of an adsorbed self-assembled monolayer of low surface-energy molecules prepared on the plasmonic nanopillars. This process can be used to tune the surface energy and provide a superhydrophobic surface with a water contact angle of 165.8° on the one hand or a hydrophilic surface with a water contact angle of 40.0° on the other. The highly tunable surface wettability is employed to systematically investigate the effects of the surface energy on the capillary-force-induced clustering among the HAR plasmonic nanopillars as well as on molecular concentration at the collapsed nanogaps present at the tops of the clustered nanopillars., This study was supported financially by the Fundamental Research Program (PNK 5060) of the Korean Institute of Materials Science (KIMS). Dr. S.-G. Park is grateful for support from the Basic Science Research Program of the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (Grant No. NRF-2015R1C1A01053884). X. Xiao is grateful for support from the Lee Family Scholars. A.B. and S.A.M. acknowledge the EPSRC and ONR Global

5 pags., 5 figs., 1 tab., The neutron-rich nuclei Nd158,160 have been studied via delayed γ-ray spectroscopy of μs isomeric states at the RIBF facility, RIKEN. These nuclei were produced following the projectile fission of a 345 AMeVU238 beam and delayed γ rays were detected by the EURICA cluster Ge array. The isomeric states have measured half-lives of 339(20) ns and 1.63(21) μs for Nd158 and Nd160, respectively. From the observed γ decays and the systematics of levels in the neighboring Nd isotopes first level schemes were constructed for these nuclei. The isomeric states of Nd158,160 have been assigned spins of (6-) and (4-), with proposed ν5/2[523] - ν7/2[633] and ν1/2[521] - ν7/2[633] configurations, respectively. ©2016 American Physical Society, We acknowledge financial support from KAKENHI (25247045). This work was carried out at the RIBF operated by RIKEN Nishina Center, RIKEN and CNS, University of Tokyo.We acknowledge the EUROBALL Owners Committee for the loan of germanium detectors and the PreSpec Collaboration for the readout electronics of the cluster detectors. Part of the WAS3ABi was supported by the Rare Isotope Science Project which is funded by the Ministry of Science, ICT and Future Planning (MSIP) and National Research Foundation (NRF) of Korea. This work was supported by the Spanish Ministerio de Ciencia e Innovación under Contracts No. FPA2009-13377-C02 and FPA2011-29854-C04 and the Hungarian Research Fund (OTKA), Contract No. K100835.

Galceran, Regina et al., Antiferromagnetic spintronics is an emerging field; antiferromagnets can improve the functionalities of ferromagnets with higher response times, and having the information shielded against external magnetic field. Moreover, a large list of aniferromagnetic semiconductors and metals with Néel temperatures above room temperature exists. In the present manuscript, we persevere in the quest for the limits of how large can anisotropic magnetoresistance be in antiferromagnetic materials with very large spin-orbit coupling. We selected IrMn as a prime example of first-class moment (Mn) and spin-orbit (Ir) combination. Isothermal magnetotransport measurements in an antiferromagnetic-metal(IrMn)/ferromagnetic-insulator thin film bilayer have been performed. The metal/insulator structure with magnetic coupling between both layers allows the measurement of the modulation of the transport properties exclusively in the antiferromagnetic layer. Anisotropic magnetoresistance as large as 0.15% has been found, which is much larger than that for a bare IrMn layer. Interestingly, it has been observed that anisotropic magnetoresistance is strongly influenced by the field cooling conditions, signaling the dependence of the found response on the formation of domains at the magnetic ordering temperature., We acknowledge financial support from the Spanish MINECO (MAT-2015-71664-R, MAT2015-73839-JIN) and FEDER program. We also acknowledge support from the ERC Advanced grant no. 268066, from the Ministry of Education of the Czech Republic Grant No. LM2015087, from the Grant Agency of the Czech Republic Grant no. 14–37427. ICMAB-CSIC authors acknowledge financial support from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV- 2015-0496). R.G. thanks the Spanish MINECO for the financial support through the FPI program and funding from Marie-Curie-ITN 607904-SPINOGRAPH. I.F. acknowledges the Beatriu de Pinós postdoctoral scholarship (2011 BP-A_2 00014) from AGAUR-Generalitat de Catalunya. B.-G.P. acknowledges support from NRF of Korea funded by the Ministry of Science, ICT & Future Planning (NRF-2014R1A2A1A11051344 and 2015M3D1A1070465). We finally thank Helmholtz-Zentrum Berlin for the allocation of synchrotron radiation beamtime, and Dr. Daniel M. Többens for his kind assistance during data collection.