1. Guidelines for May-Grünwald-Giemsa staining in haematology and non-gynaecological cytopathology: recommendations of the French Society of Clinical Cytology (SFCC) and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFA
- Author
-
Vincent S, Monique Fabre, Bellocq Jp, Michiels Jf, Françoise Thivolet, Marie-Françoise Bretz-Grenier, Philippe Vielh, Debaque H, Belleannée G, Boutonnat J, Fleury-Feith J, E. Piaton, Monique Courtade-Saïdi, Béatrix Cochand-Priollet, Egelé C, and Goubin-Versini I
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Histology ,Quality Assurance, Health Care ,May-Grunwald giemsa ,Cytodiagnosis ,MEDLINE ,Medical laboratory ,Guidelines as Topic ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Cytology ,External quality assessment ,medicine ,Humans ,Staining and Labeling ,business.industry ,Hematology ,General Medicine ,United Kingdom ,Staining ,Methylene Blue ,030104 developmental biology ,Cytopathology ,030220 oncology & carcinogenesis ,Eosine Yellowish-(YS) ,France ,business ,Quality assurance - Abstract
Objective Since the guidelines of the International Committee for Standardisation in Haematology (ICSH) in 1984 and those of the European Committee for External Quality Assessment Programmes in Laboratory Medicine (EQALM) in 2004, no leading organisation has published technical recommendations for the preparation of air-dried cytological specimens using May-Grunwald–Giemsa (MGG) staining. Data sources Literature data were retrieved using reference books, baseline-published studies, articles extracted from PubMed/Medline and Google Scholar, and online-available industry datasheets. Rationale The present review addresses all pre-analytical issues concerning the use of Romanowsky's stains (including MGG) in haematology and non-gynaecological cytopathology. It aims at serving as actualised, best practice recommendations for the proper handling of air-dried cytological specimens. It, therefore, appears complementary to the staining criteria of the non-gynaecological diagnostic cytology handbook edited by the United Kingdom National External Quality Assessment Service (UK-NEQAS) in February 2015.
- Published
- 2016