1. Preparation of 188Re-RC-160 somatostatin analog: a peptide for local/regional radiotherapy
- Author
-
Paul O. Zamora, Michael J. Marek, and Furn F. Knapp
- Subjects
Radioisotopes ,chemistry.chemical_classification ,Radiation ,Chromatography ,Radiotherapy ,Tin Compounds ,Antineoplastic Agents ,Peptide ,Stannous Tartrate ,Ascorbic acid ,High-performance liquid chromatography ,Rhenium ,Somatostatin ,chemistry ,Isotope Labeling ,Radiolysis ,Reagent Kits, Diagnostic ,Somatostatin analog ,Oxidation-Reduction ,Chromatography, High Pressure Liquid - Abstract
Conditions are described for the preparation of 188Re-RC-160, a radiolabeled synthetic peptide derived from an analogue of somatostatin, using a lyophilized labeling kit containing RC-160 (200 μg) and stannous tartrate to which is added carrier-free 188 Re (β, E max , 2,12 MeV ; γ, 155 KeV , 10%, T 1 2 = 16.7 h ) . The 3its have been radiolabeled with > 2960 Mbq (80 mCi) with a high labeling efficiency and no need for subsequent purification. Radiolabeling results in one major peak when analyzed by reverse-phase (RP) HPLC. Presumptive radiolysis was detected at 2.5 h post-labeling; however, a convenient method is described for stabilizing the kits against radiolysis by the post-labeling addition of ascorbic acid.
- Published
- 1997