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Similarity of copper and technetium binding sites in human IgG
- Source :
- International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology. 19:797-802
- Publication Year :
- 1992
- Publisher :
- Elsevier BV, 1992.
-
Abstract
- Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG.
- Subjects :
- Binding Sites
Chromatography
biology
Chemistry
Technetium
chemistry.chemical_element
Negative control
Gamma globulin
General Medicine
Binding, Competitive
Copper
Molecular biology
Immunoglobulin G
Immunoradiotherapy
In vivo
biology.protein
Humans
gamma-Globulins
Binding site
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 08832897
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology
- Accession number :
- edsair.doi.dedup.....9926824bb88206465e8ef362f54cf826