1. RNA Sequencing-Associated Study Identifies GmDRR1 as Positively Regulating the Establishment of Symbiosis in Soybean
- Author
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Lu Han, Qingying Li, Qingshan Chen, Xueying Liu, Wang Nannan, Jianan Zou, Yongxu Zhu, Zhaoming Qi, Guolong Yu, Rongsheng Zhu, Chunyan Liu, Dawei Xin, Miaoxin Guo, Yan Shi, Lin Chen, Weiyao Zhang, Shiyu Huang, Jianyi Li, Jinhui Wang, Zhenbang Hu, Chao Ma, Jingyi Zhu, Zhengong Yin, Candong Li, Yingnan Wen, Xiaoxia Wu, Zhanguo Zhang, Zhijun Sun, Hanxi Liu, Jieqi Wang, and Hongwei Jiang
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Candidate gene ,Physiology ,Effector ,food and beverages ,RNA ,RNA-Seq ,General Medicine ,Biology ,Quantitative trait locus ,01 natural sciences ,Type three secretion system ,03 medical and health sciences ,030104 developmental biology ,RNA interference ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany - Abstract
In soybean (Glycine max)-rhizobium interactions, the type III secretion system (T3SS) of rhizobium plays a key role in regulating host specificity. However, the lack of information on the role of T3SS in signaling networks limits our understanding of symbiosis. Here, we conducted an RNA sequencing analysis of three soybean chromosome segment substituted lines, one female parent and two derived lines with different chromosome-substituted segments of wild soybean and opposite nodulation patterns. By analyzing chromosome-linked differentially expressed genes in the substituted segments and quantitative trait loci (QTL)-assisted selection in the substituted-segment region, genes that may respond to type III effectors to mediate plant immunity–related signaling were identified. To narrow down the number of candidate genes, QTL assistant was used to identify the candidate region consistent with the substituted segments. Furthermore, one candidate gene, GmDRR1, was identified in the substituted segment. To investigate the role of GmDRR1 in symbiosis establishment, GmDRR1-overexpression and RNA interference soybean lines were constructed. The nodule number increased in the former compared with wild-type soybean. Additionally, the T3SS-regulated effectors appeared to interact with the GmDDR1 signaling pathway. This finding will allow the detection of T3SS-regulated effectors involved in legume-rhizobium interactions.
- Published
- 2020