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RNA Sequencing-Associated Study Identifies GmDRR1 as Positively Regulating the Establishment of Symbiosis in Soybean
- Source :
- Molecular Plant-Microbe Interactions®. 33:798-807
- Publication Year :
- 2020
- Publisher :
- Scientific Societies, 2020.
-
Abstract
- In soybean (Glycine max)-rhizobium interactions, the type III secretion system (T3SS) of rhizobium plays a key role in regulating host specificity. However, the lack of information on the role of T3SS in signaling networks limits our understanding of symbiosis. Here, we conducted an RNA sequencing analysis of three soybean chromosome segment substituted lines, one female parent and two derived lines with different chromosome-substituted segments of wild soybean and opposite nodulation patterns. By analyzing chromosome-linked differentially expressed genes in the substituted segments and quantitative trait loci (QTL)-assisted selection in the substituted-segment region, genes that may respond to type III effectors to mediate plant immunity–related signaling were identified. To narrow down the number of candidate genes, QTL assistant was used to identify the candidate region consistent with the substituted segments. Furthermore, one candidate gene, GmDRR1, was identified in the substituted segment. To investigate the role of GmDRR1 in symbiosis establishment, GmDRR1-overexpression and RNA interference soybean lines were constructed. The nodule number increased in the former compared with wild-type soybean. Additionally, the T3SS-regulated effectors appeared to interact with the GmDDR1 signaling pathway. This finding will allow the detection of T3SS-regulated effectors involved in legume-rhizobium interactions.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Candidate gene
Physiology
Effector
food and beverages
RNA
RNA-Seq
General Medicine
Biology
Quantitative trait locus
01 natural sciences
Type three secretion system
03 medical and health sciences
030104 developmental biology
RNA interference
Agronomy and Crop Science
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19437706 and 08940282
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Molecular Plant-Microbe Interactions®
- Accession number :
- edsair.doi...........c4bbbb53507922dc9e0de4a3f8bd7364