401 results on '"Meyerdierks A"'
Search Results
2. A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
- Author
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Yao, Zizhen, van Velthoven, Cindy T. J., Kunst, Michael, Zhang, Meng, McMillen, Delissa, Lee, Changkyu, Jung, Won, Goldy, Jeff, Abdelhak, Aliya, Aitken, Matthew, Baker, Katherine, Baker, Pamela, Barkan, Eliza, Bertagnolli, Darren, Bhandiwad, Ashwin, Bielstein, Cameron, Bishwakarma, Prajal, Campos, Jazmin, Carey, Daniel, Casper, Tamara, Chakka, Anish Bhaswanth, Chakrabarty, Rushil, Chavan, Sakshi, Chen, Min, Clark, Michael, Close, Jennie, Crichton, Kirsten, Daniel, Scott, DiValentin, Peter, Dolbeare, Tim, Ellingwood, Lauren, Fiabane, Elysha, Fliss, Timothy, Gee, James, Gerstenberger, James, Glandon, Alexandra, Gloe, Jessica, Gould, Joshua, Gray, James, Guilford, Nathan, Guzman, Junitta, Hirschstein, Daniel, Ho, Windy, Hooper, Marcus, Huang, Mike, Hupp, Madie, Jin, Kelly, Kroll, Matthew, Lathia, Kanan, Leon, Arielle, Li, Su, Long, Brian, Madigan, Zach, Malloy, Jessica, Malone, Jocelin, Maltzer, Zoe, Martin, Naomi, McCue, Rachel, McGinty, Ryan, Mei, Nicholas, Melchor, Jose, Meyerdierks, Emma, Mollenkopf, Tyler, Moonsman, Skyler, Nguyen, Thuc Nghi, Otto, Sven, Pham, Trangthanh, Rimorin, Christine, Ruiz, Augustin, Sanchez, Raymond, Sawyer, Lane, Shapovalova, Nadiya, Shepard, Noah, Slaughterbeck, Cliff, Sulc, Josef, Tieu, Michael, Torkelson, Amy, Tung, Herman, Valera Cuevas, Nasmil, Vance, Shane, Wadhwani, Katherine, Ward, Katelyn, Levi, Boaz, Farrell, Colin, Young, Rob, Staats, Brian, Wang, Ming-Qiang Michael, Thompson, Carol L., Mufti, Shoaib, Pagan, Chelsea M., Kruse, Lauren, Dee, Nick, Sunkin, Susan M., Esposito, Luke, Hawrylycz, Michael J., Waters, Jack, Ng, Lydia, Smith, Kimberly, Tasic, Bosiljka, Zhuang, Xiaowei, and Zeng, Hongkui
- Published
- 2023
- Full Text
- View/download PDF
3. Spontaneous coronary artery dissection in a young patient with antiphospholipid syndrome
- Author
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Ai Phi Thuy Ho, Eirik Tjønnfjord, Oliver Meyerdierks, and Ellen Elisabeth Brodin
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Antiphospholipid syndrome ,Percutaneous coronary intervention ,Spontaneous coronary artery dissection ,Chest pain ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract A 28-year-old man diagnosed with triple positive antiphospholipid syndrome (APS) and undergoing warfarin experienced three separate admissions to the cardiac ward within a one-month period due to escalating chest pain. While the initial two admissions revealed normal results in cardiological investigations, such as blood tests, electrocardiogram, and echocardiography, the third admission unveiled signs of ST-elevation myocardial infarction (STEMI), despite the patient maintaining an INR (International Normalized Ratio) of 4. Subsequent percutaneous coronary intervention (PCI) exposed spontaneous coronary artery dissection (SCAD) of type 3. Faced with hemodynamic instability and worsening symptoms, the patient underwent stenting and was prescribed dual antiplatelet therapy in addition to warfarin. A follow-up evaluation one month later indicated a normalization of his condition.
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- 2024
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4. High abundance of hydrocarbon-degrading Alcanivorax in plumes of hydrothermally active volcanoes in the South Pacific Ocean
- Author
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Dede, Bledina, Priest, Taylor, Bach, Wolfgang, Walter, Maren, Amann, Rudolf, and Meyerdierks, Anke
- Published
- 2023
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5. Niche differentiation of sulfur-oxidizing bacteria (SUP05) in submarine hydrothermal plumes
- Author
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Dede, Bledina, Hansen, Christian T., Neuholz, Rene, Schnetger, Bernhard, Kleint, Charlotte, Walker, Sharon, Bach, Wolfgang, Amann, Rudolf, and Meyerdierks, Anke
- Published
- 2022
- Full Text
- View/download PDF
6. Determination of LME sensitivity of zinc-coated steels based on the programmable deformation cracking test
- Author
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Meyerdierks, M., Zinke, M., Jüttner, S., and Biro, E.
- Published
- 2021
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- View/download PDF
7. Molecular hallmarks of Alzheimer’s disease with a multimodal single cell atlas of the cortex
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Travaglini, Kyle J, primary, Gabitto, Mariano I, additional, Ariza, Jeanelle, additional, Ding, Yi, additional, Mahoney, Joseph T, additional, Casper, Tamara, additional, Chakrabarty, Rushil, additional, Clark, Michael, additional, Crane, Paul K, additional, Dee, Nick, additional, Ferrer, Rebecca, additional, Gatto, Nicole M, additional, Gloe, Jessica, additional, Goldy, Jeff, additional, Grabowski, Thomas J, additional, Guilford, Nathan, additional, Guzman, Junitta, additional, Hawrylycz, Michael J, additional, Hodge, Rebecca D, additional, Jayadev, Suman, additional, Kaplan, Eitan S, additional, Keene, C Dirk, additional, Larson, Eric B, additional, Latimer, Caitlin S, additional, Levi, Boaz P, additional, Melief, Erica J, additional, Meyerdierks, Emma, additional, Mukherjee, Shubhabrata, additional, Pham, Thanh, additional, Rachleff, Victoria M, additional, Smith, Kimberly, additional, Torkelson, Amy, additional, Lein, Ed S, additional, and Miller, Jeremy A, additional
- Published
- 2023
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8. Bacterial communities in temperate and polar coastal sands are seasonally stable
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Miksch, Sebastian, Meiners, Mirja, Meyerdierks, Anke, Probandt, David, Wegener, Gunter, Titschack, Jürgen, Jensen, Maria A., Ellrott, Andreas, Amann, Rudolf, and Knittel, Katrin
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- 2021
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9. Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria)
- Author
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Assié, Adrien, Leisch, Nikolaus, Meier, Dimitri V., Gruber-Vodicka, Harald, Tegetmeyer, Halina E., Meyerdierks, Anke, Kleiner, Manuel, Hinzke, Tjorven, Joye, Samantha, Saxton, Matthew, Dubilier, Nicole, and Petersen, Jillian M.
- Published
- 2020
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10. Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
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Wiegand, Sandra, Jogler, Mareike, Boedeker, Christian, Pinto, Daniela, Vollmers, John, Rivas-Marín, Elena, Kohn, Timo, Peeters, Stijn H., Heuer, Anja, Rast, Patrick, Oberbeckmann, Sonja, Bunk, Boyke, Jeske, Olga, Meyerdierks, Anke, Storesund, Julia E., Kallscheuer, Nicolai, Lücker, Sebastian, Lage, Olga M., Pohl, Thomas, Merkel, Broder J., Hornburger, Peter, Müller, Ralph-Walter, Brümmer, Franz, Labrenz, Matthias, Spormann, Alfred M., Op den Camp, Huub J. M., Overmann, Jörg, Amann, Rudolf, Jetten, Mike S. M., Mascher, Thorsten, Medema, Marnix H., Devos, Damien P., Kaster, Anne-Kristin, Øvreås, Lise, Rohde, Manfred, Galperin, Michael Y., and Jogler, Christian
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- 2020
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11. High abundance of hydrocarbon-degrading Alcanivorax in plumes of hydrothermally active volcanoes in the South Pacific Ocean
- Author
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Bledina Dede, Taylor Priest, Wolfgang Bach, Maren Walter, Rudolf Amann, and Anke Meyerdierks
- Subjects
Microbiology ,Ecology, Evolution, Behavior and Systematics - Abstract
Species within the genus Alcanivorax are well known hydrocarbon-degraders that propagate quickly in oil spills and natural oil seepage. They are also inhabitants of the deep-sea and have been found in several hydrothermal plumes. However, an in-depth analysis of deep-sea Alcanivorax is currently lacking. In this study, we used multiple culture-independent techniques to analyze the microbial community composition of hydrothermal plumes in the Northern Tonga arc and Northeastern Lau Basin focusing on the autecology of Alcanivorax. The hydrothermal vents feeding the plumes are hosted in an arc volcano (Niua), a rear-arc caldera (Niuatahi) and the Northeast Lau Spreading Centre (Maka). Fluorescence in situ hybridization revealed that Alcanivorax dominated the community at two sites (1210–1565 mbsl), reaching up to 48% relative abundance (3.5 × 104 cells/ml). Through 16S rRNA gene and metagenome analyses, we identified that this pattern was driven by two Alcanivorax species in the plumes of Niuatahi and Maka. Despite no indication for hydrocarbon presence in the plumes of these areas, a high expression of genes involved in hydrocarbon-degradation was observed. We hypothesize that the high abundance and gene expression of Alcanivorax is likely due to yet undiscovered hydrocarbon seepage from the seafloor, potentially resulting from recent volcanic activity in the area. Chain-length and complexity of hydrocarbons, and water depth could be driving niche partitioning in Alcanivorax.
- Published
- 2023
12. Integrated multimodal cell atlas of Alzheimer’s disease
- Author
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Gabitto, Mariano, primary, Travaglini, Kyle, additional, Ariza, Jeannelle, additional, Kaplan, Eitan, additional, Long, Brian, additional, Rachleff, Victoria, additional, Ding, Yi, additional, Mahoney, Joseph, additional, Dee, Nick, additional, Goldy, Jeff, additional, Melief, Erica, additional, Brouner, Krissy, additional, Compos, Jazmin, additional, Campos, John, additional, Carr, Ambrose, additional, Casper, Tamara, additional, Chakrabarty, Rushil, additional, Clark, Michael, additional, Cool, Jonah, additional, Dalley, Rachel, additional, Darvas, Martin, additional, Dolbeare, Tim, additional, Ding, Song-Lin, additional, Egdorf, Tom, additional, Esposito, Luke, additional, Ferrer, Rebecca, additional, Gala, Rohan, additional, Gary, Amanda, additional, Gloe, Jessica, additional, Guilford, Nathan, additional, Guzman, Junitta, additional, Ho, Windy, additional, Jarsky, Tim, additional, Johansen, Nelson, additional, Kalmbach, Brian, additional, Keene, Lisa, additional, Khawand, Sarah, additional, Kilgore, Mitchell, additional, Kirkland, Amanda, additional, Kunst, Michael, additional, Lee, Brian, additional, Donald, Christine Mac, additional, Malone, Jocelin, additional, Maltzer, Zoe, additional, Martin, Naomi, additional, McCue, Rachel, additional, McMillen, Delissa, additional, Meyerdierks, Emma, additional, Meyers, Kelly, additional, Mollenkopf, Tyler, additional, Montine, Mark, additional, Nolan, Amber, additional, Nyhus, Julie, additional, Olsen, Paul, additional, Pacleb, Maiya, additional, Pham, Trangthanh, additional, Pom, Christina, additional, Postupna, Nadia, additional, Ruiz, Augustin, additional, Schantz, Aimee, additional, Sorensen, Staci, additional, Staats, Brian, additional, Sullivan, Matt, additional, Sunkin, Susan, additional, Thompson, Carol, additional, Tieu, Michael, additional, Ting, Jonathan, additional, Torkelson, Amy, additional, Tran, Tracy, additional, Cuevas, Nasmil Valera, additional, Wang, Ming-Qiang, additional, Waters, Jack, additional, Wilson, Angela, additional, Haynor, David, additional, Gatto, Nicole, additional, Jayadev, Suman, additional, Mufti, Shoaib, additional, Ng, Lydia, additional, Mukherjee, Shubhabrata, additional, Crane, Paul, additional, Latimer, Caitlin, additional, Levi, Boaz, additional, Smith, Kimberly, additional, Close, Jennie, additional, Miller, Jeremy, additional, Hodge, Rebecca, additional, Larson, Eric, additional, Grabowski, Thomas, additional, Hawrylycz, Michael, additional, Keene, C., additional, and Lein, Ed, additional
- Published
- 2023
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13. Integrated multimodal cell atlas of Alzheimer’s disease
- Author
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Gabitto, Mariano I., primary, Travaglini, Kyle J., additional, Rachleff, Victoria M., additional, Kaplan, Eitan S., additional, Long, Brian, additional, Ariza, Jeanelle, additional, Ding, Yi, additional, Mahoney, Joseph T., additional, Dee, Nick, additional, Goldy, Jeff, additional, Melief, Erica J., additional, Brouner, Krissy, additional, Campos, Jazmin, additional, Campos, John, additional, Carr, Ambrose J., additional, Casper, Tamara, additional, Chakrabarty, Rushil, additional, Clark, Michael, additional, Cool, Jonah, additional, Valera Cuevas, Nasmil J., additional, Dalley, Rachel, additional, Darvas, Martin, additional, Ding, Song-Lin, additional, Dolbeare, Tim, additional, Mac Donald, Christine L., additional, Egdorf, Tom, additional, Esposito, Luke, additional, Ferrer, Rebecca, additional, Gala, Rohan, additional, Gary, Amanda, additional, Gloe, Jessica, additional, Guilford, Nathan, additional, Guzman, Junitta, additional, Hirschstein, Daniel, additional, Ho, Windy, additional, Jarksy, Tim, additional, Johansen, Nelson, additional, Kalmbach, Brian E., additional, Keene, Lisa M., additional, Khawand, Sarah, additional, Kilgore, Mitch, additional, Kirkland, Amanda, additional, Kunst, Michael, additional, Lee, Brian R., additional, Malone, Jocelin, additional, Maltzer, Zoe, additional, Martin, Naomi, additional, McCue, Rachel, additional, McMillen, Delissa, additional, Meyerdierks, Emma, additional, Meyers, Kelly P., additional, Mollenkopf, Tyler, additional, Montine, Mark, additional, Nolan, Amber L., additional, Nyhus, Julie, additional, Olsen, Paul A., additional, Pacleb, Maiya, additional, Peña, Nicholas, additional, Pham, Thanh, additional, Pom, Christina Alice, additional, Postupna, Nadia, additional, Ruiz, Augustin, additional, Schantz, Aimee M., additional, Shapovalova, Nadiya V., additional, Sorensen, Staci A., additional, Staats, Brian, additional, Sullivan, Matt, additional, Sunkin, Susan M., additional, Thompson, Carol, additional, Tieu, Michael, additional, Ting, Jonathan, additional, Torkelson, Amy, additional, Tran, Tracy, additional, Wang, Ming-Qiang, additional, Waters, Jack, additional, Wilson, Angela M., additional, Haynor, David, additional, Gatto, Nicole, additional, Jayadev, Suman, additional, Mufti, Shoaib, additional, Ng, Lydia, additional, Mukherjee, Shubhabrata, additional, Crane, Paul K., additional, Latimer, Caitlin S., additional, Levi, Boaz P., additional, Smith, Kimberly, additional, Close, Jennie L., additional, Miller, Jeremy A., additional, Hodge, Rebecca D., additional, Larson, Eric B., additional, Grabowski, Thomas J., additional, Hawrylycz, Michael, additional, Dirk Keene, C., additional, and Lein, Ed S., additional
- Published
- 2023
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14. Integrated multimodal cell atlas of Alzheimer’s disease
- Author
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Mariano Gabitto, Kyle Travaglini, Jeannelle Ariza, Eitan Kaplan, Brian Long, Victoria Rachleff, Yi Ding, Joseph Mahoney, Nick Dee, Jeff Goldy, Erica Melief, Krissy Brouner, Jazmin Compos, John Campos, Ambrose Carr, Tamara Casper, Rushil Chakrabarty, Michael Clark, Jonah Cool, Rachel Dalley, Martin Darvas, Tim Dolbeare, Song-Lin Ding, Tom Egdorf, Luke Esposito, Rebecca Ferrer, Rohan Gala, Amanda Gary, Jessica Gloe, Nathan Guilford, Junitta Guzman, Windy Ho, Tim Jarsky, Nelson Johansen, Brian Kalmbach, Lisa Keene, Sarah Khawand, Mitchell Kilgore, Amanda Kirkland, Michael Kunst, Brian Lee, Christine Mac Donald, Jocelin Malone, Zoe Maltzer, Naomi Martin, Rachel McCue, Delissa McMillen, Emma Meyerdierks, Kelly Meyers, Tyler Mollenkopf, Mark Montine, Amber Nolan, Julie Nyhus, Paul Olsen, Maiya Pacleb, Trangthanh Pham, Christina Pom, Nadia Postupna, Augustin Ruiz, Aimee Schantz, Staci Sorensen, Brian Staats, Matt Sullivan, Susan Sunkin, Carol Thompson, Michael Tieu, Jonathan Ting, Amy Torkelson, Tracy Tran, Nasmil Valera Cuevas, Ming-Qiang Wang, Jack Waters, Angela Wilson, David Haynor, Nicole Gatto, Suman Jayadev, Shoaib Mufti, Lydia Ng, Shubhabrata Mukherjee, Paul Crane, Caitlin Latimer, Boaz Levi, Kimberly Smith, Jennie Close, Jeremy Miller, Rebecca Hodge, Eric Larson, Thomas Grabowski, Michael Hawrylycz, C. Keene, and Ed Lein
- Abstract
Alzheimer’s disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive stereotyped accumulation of protein aggregates, but the underlying molecular and cellular mechanisms driving AD progression and vulnerable cell populations affected by disease remain coarsely understood. The current study harnesses the BRAIN Initiative Cell Census Network experimental practices, combining quantitative neuropathology with single cell genomics and spatial transcriptomics, to understand the impact of disease progression on middle temporal gyrus cell types. We used quantitative neuropathology to place 84 cases spanning the spectrum of AD pathology along a continuous disease pseudoprogression score. We used multiomic technologies to profile single nuclei from each donor, mapping their identity to a common cell type reference with unprecedented resolution. Temporal analysis of cell-type proportions indicated an early reduction of Somatostatin-expressing neuronal subtypes and a late decrease of supragranular intratelencephalic-projecting excitatory and Parvalbumin-expressing neurons, with increases in disease-associated microglial and astrocytic states. We found complex gene expression differences, ranging from global to cell type-specific effects. These effects showed different temporal patterns indicating diverse cellular perturbations as a function of disease progression. A subset of donors showed a particularly severe cellular and molecular phenotype, which correlated with steeper cognitive decline. We have created a freely available public resource to explore these data and to accelerate progress in AD research at SEA-AD.org.
- Published
- 2023
15. Integrated multimodal cell atlas of Alzheimer’s disease
- Author
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Mariano I. Gabitto, Kyle J. Travaglini, Victoria M. Rachleff, Eitan S. Kaplan, Brian Long, Jeanelle Ariza, Yi Ding, Joseph T. Mahoney, Nick Dee, Jeff Goldy, Erica J. Melief, Krissy Brouner, Jazmin Campos, John Campos, Ambrose J. Carr, Tamara Casper, Rushil Chakrabarty, Michael Clark, Jonah Cool, Nasmil J. Valera Cuevas, Rachel Dalley, Martin Darvas, Song-Lin Ding, Tim Dolbeare, Christine L. Mac Donald, Tom Egdorf, Luke Esposito, Rebecca Ferrer, Rohan Gala, Amanda Gary, Jessica Gloe, Nathan Guilford, Junitta Guzman, Daniel Hirschstein, Windy Ho, Tim Jarksy, Nelson Johansen, Brian E. Kalmbach, Lisa M. Keene, Sarah Khawand, Mitch Kilgore, Amanda Kirkland, Michael Kunst, Brian R. Lee, Jocelin Malone, Zoe Maltzer, Naomi Martin, Rachel McCue, Delissa McMillen, Emma Meyerdierks, Kelly P. Meyers, Tyler Mollenkopf, Mark Montine, Amber L. Nolan, Julie Nyhus, Paul A. Olsen, Maiya Pacleb, Nicholas Peña, Thanh Pham, Christina Alice Pom, Nadia Postupna, Augustin Ruiz, Aimee M. Schantz, Nadiya V. Shapovalova, Staci A. Sorensen, Brian Staats, Matt Sullivan, Susan M. Sunkin, Carol Thompson, Michael Tieu, Jonathan Ting, Amy Torkelson, Tracy Tran, Ming-Qiang Wang, Jack Waters, Angela M. Wilson, David Haynor, Nicole Gatto, Suman Jayadev, Shoaib Mufti, Lydia Ng, Shubhabrata Mukherjee, Paul K. Crane, Caitlin S. Latimer, Boaz P. Levi, Kimberly Smith, Jennie L. Close, Jeremy A. Miller, Rebecca D. Hodge, Eric B. Larson, Thomas J. Grabowski, Michael Hawrylycz, C. Dirk Keene, and Ed S. Lein
- Abstract
Alzheimer’s disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive and stereotyped accumulation of protein aggregates, but the underlying molecular and cellular mechanisms driving AD progression and vulnerable cell populations affected by disease remain coarsely understood. The current study harnesses single cell and spatial genomics tools and knowledge from the BRAIN Initiative Cell Census Network to understand the impact of disease progression on middle temporal gyrus cell types. We used image-based quantitative neuropathology to place 84 donors spanning the spectrum of AD pathology along a continuous disease pseudoprogression score and multiomic technologies to profile single nuclei from each donor, mapping their transcriptomes, epigenomes, and spatial coordinates to a common cell type reference with unprecedented resolution. Temporal analysis of cell-type proportions indicated an early reduction of Somatostatin-expressing neuronal subtypes and a late decrease of supragranular intratelencephalic-projecting excitatory and Parvalbumin-expressing neurons, with increases in disease-associated microglial and astrocytic states. We found complex gene expression differences, ranging from global to cell type-specific effects. These effects showed different temporal patterns indicating diverse cellular perturbations as a function of disease progression. A subset of donors showed a particularly severe cellular and molecular phenotype, which correlated with steeper cognitive decline. We have created a freely available public resource to explore these data and to accelerate progress in AD research atSEA-AD.org.
- Published
- 2023
16. A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
- Author
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Yao, Zizhen, primary, van Velthoven, Cindy T. J., additional, Kunst, Michael, additional, Zhang, Meng, additional, McMillen, Delissa, additional, Lee, Changkyu, additional, Jung, Won, additional, Goldy, Jeff, additional, Abdelhak, Aliya, additional, Baker, Pamela, additional, Barkan, Eliza, additional, Bertagnolli, Darren, additional, Campos, Jazmin, additional, Carey, Daniel, additional, Casper, Tamara, additional, Chakka, Anish Bhaswanth, additional, Chakrabarty, Rushil, additional, Chavan, Sakshi, additional, Chen, Min, additional, Clark, Michael, additional, Close, Jennie, additional, Crichton, Kirsten, additional, Daniel, Scott, additional, Dolbeare, Tim, additional, Ellingwood, Lauren, additional, Gee, James, additional, Glandon, Alexandra, additional, Gloe, Jessica, additional, Gould, Joshua, additional, Gray, James, additional, Guilford, Nathan, additional, Guzman, Junitta, additional, Hirschstein, Daniel, additional, Ho, Windy, additional, Jin, Kelly, additional, Kroll, Matthew, additional, Lathia, Kanan, additional, Leon, Arielle, additional, Long, Brian, additional, Maltzer, Zoe, additional, Martin, Naomi, additional, McCue, Rachel, additional, Meyerdierks, Emma, additional, Nguyen, Thuc Nghi, additional, Pham, Trangthanh, additional, Rimorin, Christine, additional, Ruiz, Augustin, additional, Shapovalova, Nadiya, additional, Slaughterbeck, Cliff, additional, Sulc, Josef, additional, Tieu, Michael, additional, Torkelson, Amy, additional, Tung, Herman, additional, Cuevas, Nasmil Valera, additional, Wadhwani, Katherine, additional, Ward, Katelyn, additional, Levi, Boaz, additional, Farrell, Colin, additional, Thompson, Carol L., additional, Mufti, Shoaib, additional, Pagan, Chelsea M., additional, Kruse, Lauren, additional, Dee, Nick, additional, Sunkin, Susan M., additional, Esposito, Luke, additional, Hawrylycz, Michael J., additional, Waters, Jack, additional, Ng, Lydia, additional, Smith, Kimberly A., additional, Tasic, Bosiljka, additional, Zhuang, Xiaowei, additional, and Zeng, Hongkui, additional
- Published
- 2023
- Full Text
- View/download PDF
17. A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
- Author
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Zizhen Yao, Cindy T. J. van Velthoven, Michael Kunst, Meng Zhang, Delissa McMillen, Changkyu Lee, Won Jung, Jeff Goldy, Aliya Abdelhak, Pamela Baker, Eliza Barkan, Darren Bertagnolli, Jazmin Campos, Daniel Carey, Tamara Casper, Anish Bhaswanth Chakka, Rushil Chakrabarty, Sakshi Chavan, Min Chen, Michael Clark, Jennie Close, Kirsten Crichton, Scott Daniel, Tim Dolbeare, Lauren Ellingwood, James Gee, Alexandra Glandon, Jessica Gloe, Joshua Gould, James Gray, Nathan Guilford, Junitta Guzman, Daniel Hirschstein, Windy Ho, Kelly Jin, Matthew Kroll, Kanan Lathia, Arielle Leon, Brian Long, Zoe Maltzer, Naomi Martin, Rachel McCue, Emma Meyerdierks, Thuc Nghi Nguyen, Trangthanh Pham, Christine Rimorin, Augustin Ruiz, Nadiya Shapovalova, Cliff Slaughterbeck, Josef Sulc, Michael Tieu, Amy Torkelson, Herman Tung, Nasmil Valera Cuevas, Katherine Wadhwani, Katelyn Ward, Boaz Levi, Colin Farrell, Carol L. Thompson, Shoaib Mufti, Chelsea M. Pagan, Lauren Kruse, Nick Dee, Susan M. Sunkin, Luke Esposito, Michael J. Hawrylycz, Jack Waters, Lydia Ng, Kimberly A. Smith, Bosiljka Tasic, Xiaowei Zhuang, and Hongkui Zeng
- Subjects
Article - Abstract
The mammalian brain is composed of millions to billions of cells that are organized into numerous cell types with specific spatial distribution patterns and structural and functional properties. An essential step towards understanding brain function is to obtain a parts list, i.e., a catalog of cell types, of the brain. Here, we report a comprehensive and high-resolution transcriptomic and spatial cell type atlas for the whole adult mouse brain. The cell type atlas was created based on the combination of two single-cell-level, whole-brain-scale datasets: a single- cell RNA-sequencing (scRNA-seq) dataset of ∼7 million cells profiled, and a spatially resolved transcriptomic dataset of ∼4.3 million cells using MERFISH. The atlas is hierarchically organized into five nested levels of classification: 7 divisions, 32 classes, 306 subclasses, 1,045 supertypes and 5,200 clusters. We systematically analyzed the neuronal, non-neuronal, and immature neuronal cell types across the brain and identified a high degree of correspondence between transcriptomic identity and spatial specificity for each cell type. The results reveal unique features of cell type organization in different brain regions, in particular, a dichotomy between the dorsal and ventral parts of the brain: the dorsal part contains relatively fewer yet highly divergent neuronal types, whereas the ventral part contains more numerous neuronal types that are more closely related to each other. We also systematically characterized cell-type specific expression of neurotransmitters, neuropeptides, and transcription factors. The study uncovered extraordinary diversity and heterogeneity in neurotransmitter and neuropeptide expression and co-expression patterns in different cell types across the brain, suggesting they mediate a myriad of modes of intercellular communications. Finally, we found that transcription factors are major determinants of cell type classification in the adult mouse brain and identified a combinatorial transcription factor code that defines cell types across all parts of the brain. The whole-mouse-brain transcriptomic and spatial cell type atlas establishes a benchmark reference atlas and a foundational resource for deep and integrative investigations of cell type and circuit function, development, and evolution of the mammalian brain.
- Published
- 2023
18. Niche differentiation of sulfur-oxidizing bacteria (SUP05) in submarine hydrothermal plumes
- Author
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Bledina Dede, Christian T. Hansen, Rene Neuholz, Bernhard Schnetger, Charlotte Kleint, Sharon Walker, Wolfgang Bach, Rudolf Amann, Anke Meyerdierks, and Publica
- Subjects
Hydrothermal Vents ,Bacteria ,RNA, Ribosomal, 16S ,Seawater ,Oxidation-Reduction ,Microbiology ,In Situ Hybridization, Fluorescence ,Phylogeny ,Sulfur ,Ecology, Evolution, Behavior and Systematics - Abstract
Hydrothermal plumes transport reduced chemical species and metals into the open ocean. Despite their considerable spatial scale and impact on biogeochemical cycles, niche differentiation of abundant microbial clades is poorly understood. Here, we analyzed the microbial ecology of two bathy- (Brothers volcano; BrV-cone and northwest caldera; NWC) and a mesopelagic (Macauley volcano; McV) plumes on the Kermadec intra-oceanic arc in the South Pacific Ocean. The microbial community structure, determined by a combination of 16S rRNA gene, fluorescence in situ hybridization and metagenome analysis, was similar to the communities observed in other sulfur-rich plumes. This includes a dominance of the vent characteristic SUP05 clade (up to 22% in McV and 51% in BrV). In each of the three plumes analyzed, the community was dominated by a different yet uncultivated chemoautotrophic SUP05 species, here, provisionally named, Candidatus Thioglobus vadi (McV), Candidatus Thioglobus vulcanius (BrV-cone) and Candidatus Thioglobus plumae (BrV-NWC). Statistical analyses, genomic potential and mRNA expression profiles suggested a SUP05 niche partitioning based on sulfide and iron concentration as well as water depth. A fourth SUP05 species was present at low frequency throughout investigated plume samples and may be capable of heterotrophic or mixotrophic growth. Taken together, we propose that small variations in environmental parameters and depth drive SUP05 niche partitioning in hydrothermal plumes.
- Published
- 2022
19. Metaproteogenomic Profiling of Microbial Communities Colonizing Actively Venting Hydrothermal Chimneys
- Author
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Petra Pjevac, Dimitri V. Meier, Stephanie Markert, Christian Hentschker, Thomas Schweder, Dörte Becher, Harald R. Gruber-Vodicka, Michael Richter, Wolfgang Bach, Rudolf Amann, and Anke Meyerdierks
- Subjects
hydrothermal vent ,metagenome ,metaproteome ,sulfur cycling ,Epsilonproteobacteria ,Microbiology ,QR1-502 - Abstract
At hydrothermal vent sites, chimneys consisting of sulfides, sulfates, and oxides are formed upon contact of reduced hydrothermal fluids with oxygenated seawater. The walls and surfaces of these chimneys are an important habitat for vent-associated microorganisms. We used community proteogenomics to investigate and compare the composition, metabolic potential and relative in situ protein abundance of microbial communities colonizing two actively venting hydrothermal chimneys from the Manus Basin back-arc spreading center (Papua New Guinea). We identified overlaps in the in situ functional profiles of both chimneys, despite differences in microbial community composition and venting regime. Carbon fixation on both chimneys seems to have been primarily mediated through the reverse tricarboxylic acid cycle and fueled by sulfur-oxidation, while the abundant metabolic potential for hydrogen oxidation and carbon fixation via the Calvin–Benson–Bassham cycle was hardly utilized. Notably, the highly diverse microbial community colonizing the analyzed black smoker chimney had a highly redundant metabolic potential. In contrast, the considerably less diverse community colonizing the diffusely venting chimney displayed a higher metabolic versatility. An increased diversity on the phylogenetic level is thus not directly linked to an increased metabolic diversity in microbial communities that colonize hydrothermal chimneys.
- Published
- 2018
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20. Determination of LME sensitivity of zinc-coated steels based on the programmable deformation cracking test
- Author
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Elliot Biro, Sven Jüttner, Manuela Zinke, and M. Meyerdierks
- Subjects
Materials science ,Mechanical Engineering ,Metals and Alloys ,Test method ,Welding ,Deformation (meteorology) ,Plasticity ,law.invention ,Cracking ,Mechanics of Materials ,law ,Liquid metal embrittlement ,Arc welding ,Composite material ,Tensile testing - Abstract
The current work presents a new test method to evaluate liquid metal embrittlement (LME) susceptibility of zinc-coated steels in arc processes under application-oriented conditions. The procedure is based on the programmable deformation cracking test (PVR test). The PVR test is a variation of a controlled tensile test for hot cracking investigations in arc welding processes. Two dual-phase steels (DP600, DP980) and five transformation-induced plasticity steels (TRIP690, TRIP700, TRIP700, TRIP1100, TRIP1200) were used. The investigations showed that comparable thermo-mechanical loading conditions can be guaranteed for materials of different sheet thicknesses in the PVR test through a targeted adjustment of the heat input per unit length of weld. Furthermore, it was shown that the critical deformation rate $${v}_{cr}$$ v cr (used for assessing hot cracking susceptibility) may also be used to assess the LME susceptibility of a particular steel. Furthermore, another LME susceptibility parameter, the relative reduction in load-bearing ability $$\Delta\Sigma$$ Δ Σ could be derived, which may be used to understand how LME cracking affects materials’ mechanical and fracture properties.
- Published
- 2021
21. Metagenome Analysis
- Author
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Meyerdierks, Anke, Glöckner, Frank Oliver, Cock, J. Mark, editor, Tessmar-Raible, Kristin, editor, Boyen, Catherine, editor, and Viard, Frédérique, editor
- Published
- 2010
- Full Text
- View/download PDF
22. Metagenome Analyses
- Author
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Glöckner, Frank Oliver, Meyerdierks, Anke, and Stackebrandt, Erko, editor
- Published
- 2006
- Full Text
- View/download PDF
23. Long-Term Results After Simple Versus Complex Stenting of Coronary Artery Bifurcation Lesions: Nordic Bifurcation Study 5-Year Follow-Up Results
- Author
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Maeng, Michael, Holm, Niels R., Erglis, Andrejs, Kumsars, Indulis, Niemelä, Matti, Kervinen, Kari, Jensen, Jan S., Galløe, Anders, Steigen, Terje K., Wiseth, Rune, Narbute, Inga, Gunnes, Pål, Mannsverk, Jan, Meyerdierks, Oliver, Rotevatn, Svein, Nikus, Kjell, Vikman, Saila, Ravkilde, Jan, James, Stefan, Aarøe, Jens, Ylitalo, Antti, Helqvist, Steffen, Sjögren, Iwar, Thayssen, Per, Virtanen, Kari, Puhakka, Mikko, Airaksinen, Juhani, Christiansen, Evald H., Lassen, Jens F., and Thuesen, Leif
- Published
- 2013
- Full Text
- View/download PDF
24. Ciclosporin – eine effektive Alternative zur Behandlung von Hornhautvaskularisationen
- Author
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Meyerdierks, AL, Schuart, C, and Thieme, H
- Subjects
ddc: 610 ,Medicine and health - Abstract
Hintergrund: Verschiedene systemische oder lokal ophthalmologische Erkrankungen können zum Teil massive Hornhautvaskularisationen und dadurch bedingte Hornhauttrübungen und Visusverlust bedingen. Häufig bedeuten diese eine langfristige Therapie und einen nur sehr langsamen Heilungsverlauf. [zum vollständigen Text gelangen Sie über die oben angegebene URL]
- Published
- 2021
- Full Text
- View/download PDF
25. High abundance of hydrocarbon-degrading Alcanivoraxin plumes of hydrothermally active volcanoes in the South Pacific Ocean
- Author
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Dede, Bledina, Priest, Taylor, Bach, Wolfgang, Walter, Maren, Amann, Rudolf, and Meyerdierks, Anke
- Abstract
Species within the genus Alcanivoraxare well known hydrocarbon-degraders that propagate quickly in oil spills and natural oil seepage. They are also inhabitants of the deep-sea and have been found in several hydrothermal plumes. However, an in-depth analysis of deep-sea Alcanivoraxis currently lacking. In this study, we used multiple culture-independent techniques to analyze the microbial community composition of hydrothermal plumes in the Northern Tonga arc and Northeastern Lau Basin focusing on the autecology of Alcanivorax. The hydrothermal vents feeding the plumes are hosted in an arc volcano (Niua), a rear-arc caldera (Niuatahi) and the Northeast Lau Spreading Centre (Maka). Fluorescence in situ hybridization revealed that Alcanivoraxdominated the community at two sites (1210–1565 mbsl), reaching up to 48% relative abundance (3.5?×?104cells/ml). Through 16S rRNA gene and metagenome analyses, we identified that this pattern was driven by two Alcanivoraxspecies in the plumes of Niuatahi and Maka. Despite no indication for hydrocarbon presence in the plumes of these areas, a high expression of genes involved in hydrocarbon-degradation was observed. We hypothesize that the high abundance and gene expression of Alcanivoraxis likely due to yet undiscovered hydrocarbon seepage from the seafloor, potentially resulting from recent volcanic activity in the area. Chain-length and complexity of hydrocarbons, and water depth could be driving niche partitioning in Alcanivorax.
- Published
- 2023
- Full Text
- View/download PDF
26. Microbial metal-sulfide oxidation in inactive hydrothermal vent chimneys suggested by metagenomic and metaproteomic analyses
- Author
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Dimitri V. Meier, Anke Meyerdierks, Rudolf Amann, Sven Petersen, Stephanie Markert, Petra Pjevac, Wolfgang Bach, John Jamieson, and Thomas Schweder
- Subjects
chemistry.chemical_classification ,0303 health sciences ,biology ,Sulfide ,030306 microbiology ,fungi ,biology.organism_classification ,Microbiology ,Nitrospirae ,03 medical and health sciences ,chemistry ,13. Climate action ,Benthic zone ,Environmental chemistry ,Gammaproteobacteria ,Chimney ,14. Life underwater ,Autotroph ,Energy source ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Hydrothermal vent - Abstract
Metal-sulfides are wide-spread in marine benthic habitats. At deep-sea hydrothermal vents, they occur as massive sulfide chimneys formed by mineral precipitation upon mixing of reduced vent fluids with cold oxygenated sea water. Although microorganisms inhabiting actively venting chimneys and utilizing compounds supplied by the venting fluids are well studied, only little is known about microorganisms inhabiting inactive chimneys. In this study, we combined 16S rRNA gene-based community profiling of sulfide chimneys from the Manus Basin (SW Pacific) with radiometric dating, metagenome (n = 4) and metaproteome (n = 1) analyses. Our results shed light on potential lifestyles of yet poorly characterized bacterial clades colonizing inactive chimneys. These include sulfate-reducing Nitrospirae and sulfide-oxidizing Gammaproteobacteria dominating most of the inactive chimney communities. Our phylogenetic analysis attributed the gammaproteobacterial clades to the recently described Woeseiaceae family and the SSr-clade found in marine sediments around the world. Metaproteomic data identified these Gammaproteobacteria as autotrophic sulfide-oxidizers potentially facilitating metal-sulfide dissolution via extracellular electron transfer. Considering the wide distribution of these gammaproteobacterial clades in marine environments such as hydrothermal vents and sediments, microbially accelerated neutrophilic mineral oxidation might be a globally relevant process in benthic element cycling and a considerable energy source for carbon fixation in marine benthic habitats.
- Published
- 2019
27. Cytometric measurement of the DNA cell cycle in the presence of chlorophyll autofluorescence in marine eukaryotic phytoplankton by the blue-light excited dye YOYO-1
- Author
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Jochem, Frank J. and Meyerdierks, Doris
- Published
- 1999
28. Identification and activity of acetate-assimilating bacteria in diffuse fluids venting from two deep-sea hydrothermal systems
- Author
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Winkel, Matthias, Pjevac, Petra, Kleiner, Manuel, Littmann, Sten, Meyerdierks, Anke, Amann, Rudolf, and Mumann, Marc
- Published
- 2014
- Full Text
- View/download PDF
29. Microbial lipids reveal carbon assimilation patterns on hydrothermal sulfide chimneys
- Author
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Reeves, Eoghan P., Yoshinaga, Marcos Y., Pjevac, Petra, Goldenstein, Nadine I., Peplies, Jörg, Meyerdierks, Anke, Amann, Rudolf, Bach, Wolfgang, and Hinrichs, Kai-Uwe
- Published
- 2014
- Full Text
- View/download PDF
30. Bacterial communities in temperate and polar coastal sands are seasonally stable
- Author
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Sebastian Miksch, Mirja Meiners, Jürgen Titschack, Gunter Wegener, Rudolf Amann, Andreas Ellrott, Katrin Knittel, Maria Jensen, Anke Meyerdierks, and David Probandt
- Subjects
chemistry.chemical_classification ,0303 health sciences ,030306 microbiology ,Ecology ,fungi ,Community structure ,Pelagic zone ,General Medicine ,Ecological succession ,03 medical and health sciences ,Productivity (ecology) ,chemistry ,Benthic zone ,Phytoplankton ,Temperate climate ,Environmental science ,Organic matter ,14. Life underwater ,030304 developmental biology - Abstract
Coastal sands are biocatalytic filters for dissolved and particulate organic matter of marine and terrestrial origin, thus, acting as centers of organic matter transformation. At high temporal resolution, we accessed the variability of benthic bacterial communities over two annual cycles at Helgoland (North Sea), and compared it with seasonality of communities in Isfjorden (Svalbard, 78°N) sediments, where primary production does not occur during winter. Benthic community structure remained stable in both, temperate and polar sediments on the level of cell counts and 16S rRNA-based taxonomy. Actinobacteriota of uncultured Actinomarinales and Microtrichales were a major group, with 8 ± 1% of total reads (Helgoland) and 31 ± 6% (Svalbard). Their high activity (frequency of dividing cells 28%) and in situ cell numbers of >10% of total microbes in Svalbard sediments, suggest Actinomarinales and Microtrichales as key heterotrophs for carbon mineralization. Even though Helgoland and Svalbard sampling sites showed no phytodetritus-driven changes of the benthic bacterial community structure, they harbored significantly different communities (p r = 0.963). The temporal stability of benthic bacterial communities is in stark contrast to the dynamic succession typical of coastal waters, suggesting that pelagic and benthic bacterial communities respond to phytoplankton productivity very differently.
- Published
- 2021
31. Validierung von Methoden zur Vermeidung von Liquid Metal Embrittlement an realitätsnahen Prinzipbauteilen (IGF 21483 BG / P 1488)
- Author
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Meyerdierks, Martin, Schreiber, Vincent, Böhne, Christoph, Seitz, Georg, Jüttner, Sven, Meschut, Gerson, and Rethmeier, Michael
- Published
- 2021
- Full Text
- View/download PDF
32. Is radial artery occlusion and local vascular complications following transradial coronary procedures affected by the type of haemostasis device used? A non-inferiority Randomized Controlled Trial (RadCom trial)
- Author
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Due-Tønnessen, Nicole, primary, Egeland, Cecile H, additional, Meyerdierks, Oliver J, additional, and Opdahl, Anders, additional
- Published
- 2021
- Full Text
- View/download PDF
33. Is radial artery occlusion and local vascular complications following transradial coronary procedures affected by the type of haemostasis device used? A non-inferiority Randomized Controlled Trial (RadCom trial)
- Author
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Cecile H Egeland, Oliver Meyerdierks, Anders Opdahl, and Nicole Due-Tønnessen
- Subjects
medicine.medical_specialty ,Arterial Occlusive Diseases ,Hemorrhage ,030204 cardiovascular system & hematology ,Coronary Angiography ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Hematoma ,Randomized controlled trial ,law ,medicine.artery ,Occlusion ,medicine ,Clinical endpoint ,Humans ,030212 general & internal medicine ,Prospective Studies ,Radial artery ,Hemostatic function ,Adverse effect ,Advanced and Specialized Nursing ,Hemostasis ,business.industry ,medicine.disease ,Surgery ,Medical–Surgical Nursing ,Treatment Outcome ,Radial Artery ,Cardiology and Cardiovascular Medicine ,business ,Complication - Abstract
Aims Vascular access site complications following transradial coronary procedures are less common and severe compared to femoral approach. Radial artery occlusion is considered the main adverse effect. As radial access is gaining more acknowledgement, complication awareness, and understanding is important. The aim was to assess complication rates following transradial coronary procedures and to compare two radial compression devices in a non-inferiority randomized controlled trial. Methods and results Four hundred and ninety-nine patients were randomized to radial compression with a new device (RY Stop, n = 248) or the reference device (TR Band, n = 251) following transradial coronary procedures. Radial artery occlusion persistent at 90 days was the primary endpoint. Discomfort and accounts of vascular complications at access site were secondary endpoints. Radial artery occlusion was observed in 5% (n = 26) for the entire cohort with no difference between groups (RY Stop 6% vs. TR Band 5%; P = 0.69). Patients overall reported low levels of discomfort and the median scores were similar in both groups; RY Stop: 7 vs. TR Band: 10 (P = 0.90). There were few incidents of bleeding (7%), however, they were significantly more frequent with the RY Stop (12%) than with the TR Band (3%; P = 0.001). Few patients (4%) developed access site haematomas, and the incidence was similar in the two groups (P = 0.98). Conclusion We observed a radial artery occlusion rate of 5% at 90 days post-procedure. Access site discomfort and vascular complication rates were low. Overall, the RY Stop compression device was not inferior to the TR Band except occurrences of bleeding.
- Published
- 2020
34. Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
- Author
-
Alfred M. Spormann, Olga Jeske, Marnix H. Medema, Thorsten Mascher, Stijn H Peeters, Franz Brümmer, Mike S. M. Jetten, Peter Hornburger, Manfred Rohde, Christian Jogler, Michael Y. Galperin, Jörg Overmann, Mareike Jogler, Ralph Walter Müller, Christian Boedeker, Rudolf Amann, Anja Heuer, Daniela Pinto, Matthias Labrenz, Elena Rivas-Marín, Patrick Rast, Damien P. Devos, Sonja Oberbeckmann, Olga Maria Lage, Broder J. Merkel, Thomas Pohl, Sandra Wiegand, Timo Kohn, Anke Meyerdierks, Lise Øvreås, John Vollmers, Julia E. Storesund, Anne-Kristin Kaster, Sebastian Lücker, Boyke Bunk, Nicolai Kallscheuer, Huub J. M. Op den Camp, German Research Foundation, Volkswagen Foundation, National Institutes of Health (US), Medical Library Association, Federal Ministry of Science, Research and Economy (Germany), Max Planck Society, and Centro Interdisciplinar de Investigação Marinha e Ambiental
- Subjects
cell division ,ved/biology.organism_classification_rank.species ,Secondary Metabolism ,genetic analysis ,phylogeny ,Applied Microbiology and Biotechnology ,taxonomy ,Laboratory of Geo-information Science and Remote Sensing ,bacterial genome ,RNA, Ribosomal, 16S ,genetics ,Clade ,Phylogeny ,cladistics ,0303 health sciences ,biology ,bacterial structures ,species habitat ,Planctomycetes ,Bacterial Physiological Phenomena ,bacterium ,3. Good health ,priority journal ,classification ,bacterium identification ,aquatic environment ,Cell Division ,signal transduction ,Signal Transduction ,Microbiology (medical) ,amino acid analysis ,RNA 16S ,Bioinformatics ,Immunology ,bacterium culture ,Bacterial genome size ,gene sequence ,Microbiology ,DNA sequencing ,Article ,03 medical and health sciences ,Phylogenetics ,Bioinformatica ,Genetics ,Life Science ,Laboratorium voor Geo-informatiekunde en Remote Sensing ,14. Life underwater ,Model organism ,bacterial phenomena and functions ,Ecosystem ,030304 developmental biology ,Ecological niche ,growth, development and aging ,nonhuman ,electron microscopy ,Bacteria ,030306 microbiology ,ved/biology ,Genetic Variation ,Cell Biology ,biology.organism_classification ,bacterial strain ,bacterial cell ,Evolutionary biology ,Ecological Microbiology ,microbial diversity ,cytology ,EPS ,Genome, Bacterial ,cell structure - Abstract
When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection., This work was funded by the Deutsche Forschungsgemeinschaft (grant no. JO 893/4-1) and the Volkswagen foundation (experiment no. 89256). M.Y.G. was funded by the NIH IRP at the US National Library of Medicine. Work in the Mascher lab was supported by the Deutsche Forschungsgemeinschaft (grant no. MA2837/2-2) and the Bundeministerium für Bildung und Forschung in the framework of the ERAnet Synthetic Biology (project: ERASynBio2-ECFexpress). R.A. and A.M. were funded by the Max Planck Society.
- Published
- 2020
35. Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
- Author
-
Wiegand, S., Jogler, Mareike, Boedeker, Christian, Pinto, Daniela, Vollmers, John, Rivas-Marín, Elena, Kohn, T., Peeters, S.H., Heuer, Anja, Rast, P., Oberbeckmann, Sonja, Bunk, Boyke, Jeske, Olga, Meyerdierks, Anke, Storesund, Julia E., Kallscheuer, N., Lücker, S., Lage, Olga M., Pohl, Thomas, Merkel, Broder J., Hornburger, Peter, Müller, Ralph-Walter, Brümmer, Franz, Labrenz, Matthias, Spormann, Alfred M., Camp, H.J.M. op den, Overmann, Jörg, Amann, Rudolf, Jetten, M.S.M., Mascher, Thorsten, Medema, Marnix H., Devos, Damien P., Kaster, Anne-Kristin, Øvreås, Lise, Rohde, Manfred, Galperin, Michael Y., Jogler, C., Wiegand, S., Jogler, Mareike, Boedeker, Christian, Pinto, Daniela, Vollmers, John, Rivas-Marín, Elena, Kohn, T., Peeters, S.H., Heuer, Anja, Rast, P., Oberbeckmann, Sonja, Bunk, Boyke, Jeske, Olga, Meyerdierks, Anke, Storesund, Julia E., Kallscheuer, N., Lücker, S., Lage, Olga M., Pohl, Thomas, Merkel, Broder J., Hornburger, Peter, Müller, Ralph-Walter, Brümmer, Franz, Labrenz, Matthias, Spormann, Alfred M., Camp, H.J.M. op den, Overmann, Jörg, Amann, Rudolf, Jetten, M.S.M., Mascher, Thorsten, Medema, Marnix H., Devos, Damien P., Kaster, Anne-Kristin, Øvreås, Lise, Rohde, Manfred, Galperin, Michael Y., and Jogler, C.
- Abstract
Contains fulltext : 215881.pdf (Publisher’s version ) (Open Access)
- Published
- 2020
36. Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
- Author
-
German Research Foundation, Volkswagen Foundation, National Institutes of Health (US), Medical Library Association, Federal Ministry of Science, Research and Economy (Germany), Max Planck Society, Wiegand, Sandra, Jogler, Mareike, Boedeker, Christian, Pinto, Daniela, Vollmers, John, Rivas-Marín, Elena, Kohn, Timo, Peeters, Stijn H., Heuer, Anja, Rast, Patrick, Oberbeckmann, Sonja, Bunk, Boyke, Jeske, Olga, Meyerdierks, Anke, Storesund, Julia E., Kallscheuer, Nicolai, Lücker, Sebastian, Lage, Olga M., Pohl, Thomas, Merkel, Broder J., Hornburger, Peter, Müller, Ralph-Walter, Brümmer, Franz, Labrenz, Matthias, Spormann, Alfred M., Op den Camp, Huub J. M., Overmann, Jörg, Amann, Rudolf, Jetten, Mike S. M., Mascher, Thorsten, Medema, Marnix H., Devos, Damien P., Kaster, Anne-Kristin, Øvreås, Lise, Rohde, Manfred, Galperin, Michael Y., Jogler, Christian, German Research Foundation, Volkswagen Foundation, National Institutes of Health (US), Medical Library Association, Federal Ministry of Science, Research and Economy (Germany), Max Planck Society, Wiegand, Sandra, Jogler, Mareike, Boedeker, Christian, Pinto, Daniela, Vollmers, John, Rivas-Marín, Elena, Kohn, Timo, Peeters, Stijn H., Heuer, Anja, Rast, Patrick, Oberbeckmann, Sonja, Bunk, Boyke, Jeske, Olga, Meyerdierks, Anke, Storesund, Julia E., Kallscheuer, Nicolai, Lücker, Sebastian, Lage, Olga M., Pohl, Thomas, Merkel, Broder J., Hornburger, Peter, Müller, Ralph-Walter, Brümmer, Franz, Labrenz, Matthias, Spormann, Alfred M., Op den Camp, Huub J. M., Overmann, Jörg, Amann, Rudolf, Jetten, Mike S. M., Mascher, Thorsten, Medema, Marnix H., Devos, Damien P., Kaster, Anne-Kristin, Øvreås, Lise, Rohde, Manfred, Galperin, Michael Y., and Jogler, Christian
- Abstract
When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection.
- Published
- 2020
37. Clustered genes related to sulfate respiration in uncultured prokaryotes support the theory of their concomitant horizontal transfer
- Author
-
Mussmann, Marc, Richter, Michael, Lombardot, Thierry, Meyerdierks, Anke, Kuever, Jan, Kube, Michael, Glockner, Frank Oliver, and Amann, Rudolf
- Subjects
Prokaryotes -- Genetic aspects ,Genetic transformation -- Research ,Genetic research ,Biological sciences - Abstract
The dissimilatory reduction of sulfate is an ancient metabolic process central to today's biogeochemical cycling of sulfur and carbon in marine sediments. Until now its polyphyletic distribution was most parsimoniously explained by multiple horizontal transfers of single genes rather than by a not-yet-identified 'metabolic island.' Here we provide evidence that the horizontal transfer of a gene cluster may indeed be responsible for the patchy distribution of sulfate-reducing prokaryotes (SRP) in the phylogenetic tree. We isolated three DNA fragments (32 to 41 kb) from uncultured, closely related SRP from DNA directly extracted from two distinct marine sediments. Fosmid ws39f7, and partially also fosmids ws7f8 and hr42c9, harbored a core set of essential genes for the dissimilatory reduction of sulfate, including enzymes for the reduction of sulfur intermediates and synthesis of the prosthetic group of the dissimilatory sulfite reductase. Genome comparisons suggest that encoded membrane proteins universally present among SRP are critical for electron transfer to cytoplasmic enzymes. In addition, novel, conserved hypothetical proteins that are likely involved in dissimilatory sulfate reduction were identified. Based on comparative genomics and previously published experimental evidence, a more comprehensive model of dissimilatory sulfate reduction is presented. The observed clustering of genes involved in dissimilatory sulfate reduction has not been previously found. These findings strongly support the hypothesis that genes responsible for dissimilatory sulfate reduction were concomitantly transferred in a single event among prokaryotes. The acquisition of an optimized gene set would enormously facilitate a successful implementation of a novel pathway.
- Published
- 2005
38. A conspicuous nickel protein in microbial mats that oxidize methane anaerobically
- Author
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Kruger, Martin, Meyerdierks, Anke, Glockner, Frank Oliver, Amann, Rudolf, Widdel, Friedrich, Kube, Michael, Reinhardt, Richard, Kahnt, Jorg, Bocher, Reinhard, Thauer, Rudolf K., and Shima, Seigo
- Subjects
Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Martin Krüger [1]; Anke Meyerdierks [1]; Frank Oliver Glöckner [1]; Rudolf Amann [1]; Friedrich Widdel (corresponding author) [1]; Michael Kube [2]; Richard Reinhardt [2]; Jörg Kahnt [3]; Reinhard Böcher [...]
- Published
- 2003
- Full Text
- View/download PDF
39. Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island
- Author
-
Schubbe, Sabrina, Kube, Michael, Scheffel, Andre, Wawer, Cathrin, Heyen, Udo, Meyerdierks, Anke, Madkour, Mohamed H., Mayer, Frank, Reinhardt, Richard, and Schuler, Dirk
- Subjects
Chromosome deletion -- Analysis ,Mutation (Biology) -- Analysis ,Bacterial genetics -- Research ,Biological sciences - Abstract
Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria.
- Published
- 2003
40. Bacterial sulfur cycling shapes microbial communities in surface sediments of an ultramafic hydrothermal vent field
- Author
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Schauer, Regina, Ry, Hans, Augustin, Nico, Gennerich, Hans-Hermann, Peters, Marc, Wenzhoefer, Frank, Amann, Rudolf, and Meyerdierks, Anke
- Published
- 2011
- Full Text
- View/download PDF
41. Bacterial enzymes for dissimilatory sulfate reduction in a marine microbial mat (Black Sea) mediating anaerobic oxidation of methane
- Author
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Basen, Mirko, Krüger, Martin, Milucka, Jana, Kuever, Jan, Kahnt, Jörg, Grundmann, Olav, Meyerdierks, Anke, Widdel, Friedrich, and Shima, Seigo
- Published
- 2011
- Full Text
- View/download PDF
42. Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade
- Author
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Schreiber, Lars, Holler, Thomas, Knittel, Katrin, Meyerdierks, Anke, and Amann, Rudolf
- Published
- 2010
- Full Text
- View/download PDF
43. Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
- Author
-
Wiegand, Sandra, primary, Jogler, Mareike, additional, Boedeker, Christian, additional, Pinto, Daniela, additional, Vollmers, John, additional, Rivas-Marín, Elena, additional, Kohn, Timo, additional, Peeters, Stijn H., additional, Heuer, Anja, additional, Rast, Patrick, additional, Oberbeckmann, Sonja, additional, Bunk, Boyke, additional, Jeske, Olga, additional, Meyerdierks, Anke, additional, Storesund, Julia E., additional, Kallscheuer, Nicolai, additional, Lücker, Sebastian, additional, Lage, Olga M., additional, Pohl, Thomas, additional, Merkel, Broder J., additional, Hornburger, Peter, additional, Müller, Ralph-Walter, additional, Brümmer, Franz, additional, Labrenz, Matthias, additional, Spormann, Alfred M., additional, Op den Camp, Huub J. M., additional, Overmann, Jörg, additional, Amann, Rudolf, additional, Jetten, Mike S. M., additional, Mascher, Thorsten, additional, Medema, Marnix H., additional, Devos, Damien P., additional, Kaster, Anne-Kristin, additional, Øvreås, Lise, additional, Rohde, Manfred, additional, Galperin, Michael Y., additional, and Jogler, Christian, additional
- Published
- 2019
- Full Text
- View/download PDF
44. Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group
- Author
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Meyerdierks, Anke, Kube, Michael, Kostadinov, Ivaylo, Teeling, Hanno, Glöckner, Frank Oliver, Reinhardt, Richard, and Amann, Rudolf
- Published
- 2010
- Full Text
- View/download PDF
45. Niche partitioning of diverse sulfur-oxidizing bacteria at hydrothermal vents
- Author
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Stéphane Hourdez, Dimitri V. Meier, Wolfgang Bach, Rudolf Amann, Charles Vidoudez, Peter R. Girguis, Petra Pjevac, and Anke Meyerdierks
- Subjects
0301 basic medicine ,Biology ,Microbiology ,Hydrothermal circulation ,Carbon Cycle ,03 medical and health sciences ,Hydrothermal Vents ,Microbial ecology ,Sulfurimonas ,RNA, Ribosomal, 16S ,Environmental Microbiology ,Seawater ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Epsilonproteobacteria ,Sulfur Compounds ,Ecology ,Geomicrobiology ,Oxides ,Bacterioplankton ,biology.organism_classification ,030104 developmental biology ,Environmental chemistry ,Metagenome ,Original Article ,Oxidation-Reduction ,Genome, Bacterial ,Sulfur ,geographic locations ,Hydrothermal vent - Abstract
At deep-sea hydrothermal vents, primary production is carried out by chemolithoautotrophic microorganisms, with the oxidation of reduced sulfur compounds being a major driver for microbial carbon fixation. Dense and highly diverse assemblies of sulfur-oxidizing bacteria (SOB) are observed, yet the principles of niche differentiation between the different SOB across geochemical gradients remain poorly understood. In this study niche differentiation of the key SOB was addressed by extensive sampling of active sulfidic vents at six different hydrothermal venting sites in the Manus Basin, off Papua New Guinea. We subjected 33 diffuse fluid and water column samples and 23 samples from surfaces of chimneys, rocks and fauna to a combined analysis of 16S rRNA gene sequences, metagenomes and real-time in situ measured geochemical parameters. We found Sulfurovum Epsilonproteobacteria mainly attached to surfaces exposed to diffuse venting, while the SUP05-clade dominated the bacterioplankton in highly diluted mixtures of vent fluids and seawater. We propose that the high diversity within Sulfurimonas- and Sulfurovum-related Epsilonproteobacteria observed in this study derives from the high variation of environmental parameters such as oxygen and sulfide concentrations across small spatial and temporal scales.
- Published
- 2017
46. Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide
- Author
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Strittmatter, Axel W., Liesegang, Heiko, Rabus, Ralf, Decker, Iwona, Amann, Judith, Andres, Sönke, Henne, Anke, Fricke, Wolfgang Florian, Martinez-Arias, Rosa, Bartels, Daniela, Goesmann, Alexander, Krause, Lutz, Pühler, Alfred, Klenk, Hans-Peter, Richter, Michael, Schüler, Margarete, Glöckner, Frank Oliver, Meyerdierks, Anke, Gottschalk, Gerhard, and Amann, Rudolf
- Published
- 2009
- Full Text
- View/download PDF
47. Glaukomdiagnostische Herausforderungen bei Fundus heterotopicus – Ein Fall aus der Klinik
- Author
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Meyerdierks, AL, Wecke, T, and Thieme, H
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
Hintergrund: Beim Fundus heterotopicus besteht eine ein- oder beidseitige Bulbusektasie, ein schräger Sehnerveneintritt und ein Conus inferior. Die sektorförmige Bulbusektasie kann in der Sonografie nachgewiesen werden, liegt meist nasal unten und führt konsekutiv zu meist temporal oben[zum vollständigen Text gelangen Sie über die oben angegebene URL], 27. Jahrestagung der Regionalgesellschaft der Augenärzte Sachsen-Anhalts und Thüringens e. V.
- Published
- 2019
- Full Text
- View/download PDF
48. Microbial metal-sulfide oxidation in inactive hydrothermal vent chimneys suggested by metagenomic and metaproteomic analyses
- Author
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Meier, Dimitri V., Pjevac, Petra, Bach, Wolfgang, Markert, Stephanie, Schweder, Thomas, Jamieson, John, Petersen, Sven, Amann, Rudolf, and Meyerdierks, Anke
- Subjects
fungi - Abstract
Metal-sulfides are wide-spread in marine benthic habitats. At deep-sea hydrothermal vents, they occur as massive sulfide chimneys formed by mineral precipitation upon mixing of reduced vent fluids with cold oxygenated sea water. Although microorganisms inhabiting actively venting chimneys and utilizing compounds supplied by the venting fluids are well studied, only little is known about microorganisms inhabiting inactive chimneys. In this study, we combined 16S rRNA gene-based community profiling of sulfide chimneys from the Manus Basin (SW Pacific) with radiometric dating, metagenome (n = 4) and metaproteome (n = 1) analyses. Our results shed light on potential lifestyles of yet poorly characterized bacterial clades colonizing inactive chimneys. These include sulfate-reducing Nitrospirae and sulfide-oxidizing Gammaproteobacteria dominating most of the inactive chimney communities. Our phylogenetic analysis attributed the gammaproteobacterial clades to the recently described Woeseiaceae family and the SSr-clade found in marine sediments around the world. Metaproteomic data identified these Gammaproteobacteria as autotrophic sulfide-oxidizers potentially facilitating metal-sulfide dissolution via extracellular electron transfer. Considering the wide distribution of these gammaproteobacterial clades in marine environments such as hydrothermal vents and sediments, microbially accelerated neutrophilic mineral oxidation might be a globally relevant process in benthic element cycling and a considerable energy source for carbon fixation in marine benthic habitats.
- Published
- 2019
49. Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria)
- Author
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Harald R. Gruber-Vodicka, Adrien Assié, Anke Meyerdierks, Manuel Kleiner, Dimitri V. Meier, Nicole Dubilier, Jillian M. Petersen, Samantha B. Joye, Halina E. Tegetmeyer, Matthew A. Saxton, Nikolaus Leisch, and Tjorven Hinzke
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Water microbiology ,Citric Acid Cycle ,Reductive tricarboxylic acid cycle ,Microbiology ,Article ,Carbon Cycle ,Carbon cycle ,Microbial ecology ,03 medical and health sciences ,Bacterial genetics ,Gammaproteobacteria ,Animals ,Autotroph ,Photosynthesis ,Symbiosis ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Betaproteobacteria ,030304 developmental biology ,0303 health sciences ,Epsilonproteobacteria ,biology ,Phylum ,030306 microbiology ,Carbon fixation ,biology.organism_classification ,Bivalvia ,Evolutionary biology ,Metaproteomics ,Molecular evolution ,Metagenomics - Abstract
Most autotrophs use the Calvin–Benson–Bassham (CBB) cycle for carbon fixation. In contrast, all currently described autotrophs from the Campylobacterota (previously Epsilonproteobacteria) use the reductive tricarboxylic acid cycle (rTCA) instead. We discovered campylobacterotal epibionts (“CandidatusThiobarba”) of deep-sea mussels that have acquired a complete CBB cycle and may have lost most key genes of the rTCA cycle. Intriguingly, the phylogenies of campylobacterotal CBB cycle genes suggest they were acquired in multiple transfers from Gammaproteobacteria closely related to sulfur-oxidizing endosymbionts associated with the mussels, as well as from Betaproteobacteria. We hypothesize that “Ca. Thiobarba” switched from the rTCA cycle to a fully functional CBB cycle during its evolution, by acquiring genes from multiple sources, including co-occurring symbionts. We also found key CBB cycle genes in free-living Campylobacterota, suggesting that the CBB cycle may be more widespread in this phylum than previously known. Metatranscriptomics and metaproteomics confirmed high expression of CBB cycle genes in mussel-associated “Ca. Thiobarba”. Direct stable isotope fingerprinting showed that “Ca. Thiobarba” has typical CBB signatures, suggesting that it uses this cycle for carbon fixation. Our discovery calls into question current assumptions about the distribution of carbon fixation pathways in microbial lineages, and the interpretation of stable isotope measurements in the environment.
- Published
- 2019
50. Metagenomic approach to the study of halophages: the environmental halophage 1
- Author
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Santos, Fernando, Meyerdierks, Anke, Peña, Arantxa, Rosselló-Mora, Ramon, Amann, Rudolf, and Antón, Josefa
- Published
- 2007
- Full Text
- View/download PDF
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