1. Measurement of Biochemical Methane Potential of Heterogeneous Solid Substrates: Results of a Two-Phase French Inter-Laboratory Study
- Author
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Thierry Ribeiro, Romain Cresson, Sébastien Pommier, Sébastien Preys, Laura André, Fabrice Béline, Théodore Bouchez, Claire Bougrier, Pierre Buffière, Jesús Cacho, Patricia Camacho, Laurent Mazéas, André Pauss, Philippe Pouech, Maxime Rouez, and Michel Torrijos
- Subjects
BMP ,methanogenic potential ,inter-laboratory study ,solid substrates ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
Biochemical methane potential (BMP) is essential to determine the production of methane for various substrates; literature shows important discrepancies for the same substrates. In this paper, a harmonized BMP protocol was developed and tested with two phases of BMP tests carried out by eleven French laboratories. Surprisingly, for the three same solid tested substrates (straw; raw mix and dried-shredded mix of potatoes, maize, beef meat and straw; and mayonnaise), the standard deviations of the repeatability and reproducibility inter-laboratory were not enhanced by the harmonized protocol (average of about 25% depending on the substrate), as compared to a previous step where all laboratories used their own protocols. Moreover, statistical analyses of all the results, after removal of the outliers (about 15% of all observations), did not highlight significant effect of the operational effect on BMP (stirring, automatic or manual gas quantification, use of trace metal, uses a bicarbonate buffer, inoculum to substrate ratio) at least for the tested ranges. On the other hand, the average intra-laboratory repeatability was low, about 7%, whatever the protocol, the substrate and the laboratory. It also appears that drying the SA substrate, which contained proteins, carbohydrates, lipids and fibers, does not impact its BMP.
- Published
- 2020
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