1. Transient Non-Native Helix Formation during the Folding of b-Lactoglobulin
- Author
-
Masamichi Ikeguchi
- Subjects
a-helix ,b-sheet ,stopped-flow ,circular dichroism ,hydrogen-deuterium exchange ,mutant protein ,Microbiology ,QR1-502 - Abstract
In ideal proteins, only native interactions are stabilized step-by-step in a smooth funnel-like energy landscape. In real proteins, however, the transient formation of non-native structures is frequently observed. In this review, the transient formation of non-native structures is described using the non-native helix formation during the folding of b-lactoglobulin as a prominent example. Although b-lactoglobulin is a predominantly b-sheet protein, it has been shown to form non-native helices during the early stage of folding. The location of non-native helices, their stabilization mechanism, and their role in the folding reaction are discussed.
- Published
- 2014
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