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Effect of non-native helix destabilization on the folding of equine β-lactoglobulin
- Source :
- Journal of biochemistry. 156(5)
- Publication Year :
- 2014
-
Abstract
- β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to that of the wild-type protein; its folding kinetics was investigated by stopped-flow circular dichroism (CD) and fluorescence. Although A123T showed a reduced burst-phase CD intensity, its folding rate was similar to that of the wild-type protein, which indicated that the formation of the non-native helix does not accelerate or decelerate the folding reaction.
Details
- ISSN :
- 17562651
- Volume :
- 156
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of biochemistry
- Accession number :
- edsair.doi.dedup.....5bb3611f228da44b3f81b0848fd753b6