1. Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: A novel tailored treatment strategy
- Author
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Laura Bianchi, Debora Maruca, Vito Trinchieri, Eugenio Nelson Cavallari, Massimiliano De Angelis, Paolo Vassalini, Fabrizia Toscanella, S. Venosi, Ombretta Martinelli, Gabriella d'Ettorre, Alessandra Oliva, Giancarlo Ceccarelli, Luca Laghi, Venosi S., Ceccarelli G., De Angelis M., Laghi L., Bianchi L., Martinelli O., Maruca D., Cavallari E.N., Toscanella F., Vassalini P., Trinchieri V., Oliva A., and D'Ettorre G.
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Administration, Topical ,030106 microbiology ,Bacteriotherapy ,topical probiotic ,metabolomic ,wound ,wound care ,wound healing ,antimicrobial resistance ,lcsh:Medicine ,Wound healing ,Metabolomic ,Antimicrobial resistance ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,Lesion ,Pathogenesis ,Translational Research, Biomedical ,03 medical and health sciences ,Probiotic ,Wound care ,law ,Ischemia ,Internal medicine ,Skin Ulcer ,Topical probiotic ,Wound ,medicine ,Humans ,Skin ,Aged, 80 and over ,Leg ,business.industry ,Probiotics ,Research ,lcsh:R ,General Medicine ,Critical limb ischemia ,medicine.disease ,030104 developmental biology ,Chronic Disease ,Metabolome ,Wound Infection ,Female ,medicine.symptom ,business ,Dysbiosis - Abstract
Background A wide debate is ongoing regarding the role of cutaneous dysbiosis in the pathogenesis and evolution of difficult-to-treat chronic wounds. Nowadays, probiotic treatment considered as an useful tool to counteract dysbiosis but the evidence in regard to their therapeutic use in the setting of difficult-to-treat cutaneous ulcers is still poor. Aim: clinical report An 83-year-old woman suffering a critical limb ischemia and an infected difficult-to-treat ulcerated cutaneous lesion of the right leg, was complementary treated with local application of a mixture of probiotic bacteria. Methods Microbiological and metabolomic analysis were conducted on wound swabs obtained before and after bacteriotherapy. Results During the treatment course, a progressive healing of the lesion was observed with microbiological resolution of the polymicrobial infection of the wound. Metabolomic analysis showed a significant difference in the local concentration of propionate, 2-hydroxyisovalerate, 2-oxoisocaproate, 2,3-butanediol, putrescine, thymine, and trimethylamine before and after bacteriotherapy. Conclusion The microbiological and metabolomic results seem to confirm the usefulness of complementary probiotic treatment in difficult-to-treat infected wounds. Further investigations are needed to confirm these preliminary findings.
- Published
- 2019