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A 21-year-old patient experienced asthmatic attacks when cleaning the cage of his budgerigar. Skin tests and radioallergosorbent test were positive to grass pollen and negative for budgerigar feathers and feces. When the food of the bird, a mixture of grain, was tested, a positive reaction to millet was found. Nasal provocation test with millet was positive. Specific immunoglobulin E antibodies against millet were detected in the radioallergosorbent test and in immunoblot analysis. The immunoblot showed specific immunoglobulin E antibodies against a 60-kd protein in millet of birdseed and against a 60-and 36-kd protein in common millet. Immediate-type allergy to millet is rare and occurs mostly as anaphylactic reaction after ingestion of millet but may also occur as asthmatic attack after inhalation of millet. Keywords: bronchial asthma, type I allergy, millet, bird food

Guidance on efficient methods is needed for the practical application of modeling the sound field from broadband sources such as vessels, seismic surveys, and construction activities. These sound field models are employed for estimating how changes in the soundscape will affect marine life. For efficiency, acoustic propagation modeling is often performed in bands (decidecade or 13-octave), where propagation loss modeled for central frequency is assumed to represent an average propagation loss in the band. This shortcut comes at the expense of accuracy, which can be rectified by averaging the propagation loss across many frequencies in the band. Alternately, the equivalence of range and frequency averaging was shown by Harrison and Harrison [J. Acoust. Soc. Am. 97, 1314-1317 (1995)]. However, when and how to apply range averaging required further investigations. A simple environment with a flat sandy bottom and an isovelocity water-column sound speed profile was considered to test the agreement between the range and frequency averages for decidecade bands typically considered in soundscape modelling (10-1000 Hz). The optimal range smoothing window is a Gaussian window with a width of 10%-16% of the range from the source that switches to a width fixed beyond 20 km distance from the source., (© 2024 Acoustical Society of America.)

Models of the underwater acoustic soundscape are important for evaluating the effects of human generated sounds on marine life. The performance of models can be validated against measurements or verified against each other for consistency. A verification workshop was held to compare models that predict the soundscape from wind and vessels and estimate detection ranges for a submerged target. Eight modeling groups participated in the workshop which predicted sound levels with observation windows of 1 min and 1 km2. Substantial differences were found in how modelers computed the propagation losses for decidecade bands and estimated the source level of wind. Further investigations resulted in recommendations on best practices. Choices of temporal and spatial modeling resolution affected the estimates of metrics proportional to total sound energy more than distributions of sound pressure level. Deeper receivers were less sensitive to these parameters than shallow ones. A temporal resolution of 1 min and spatial resolution of 100 m is recommended. Models that follow the recommendations will yield similar results. The detection range of underwater targets is highly variable when the ambient noise depends on moving noise sources. Future work to verify models against data and understand model uncertainty is recommended., (© 2024 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).)

Competing Interests: Declaration of interests The authors declare no competing interests.

Purpose: This study examined the metazoan ectoparasites of the Critically Endangered giant shovelnose ray, Glaucostegus typus, in the eastern Indian Ocean., Methods: We screened 186 G. typus for ectoparasites in four coastal regions of Western Australia between 2020 and 2022: the Pilbara Region, Exmouth Gulf, Ningaloo Coast and Shark Bay., Results: Five parasite taxa were encountered on 186 G. typus: Caligus furcisetifer (Copepoda: Caligidae), Dermopristis cairae (Monopisthocotyla: Microbothriidae), Branchellion plicobranchus and Stibarobdella macrothela (Hirudinida: Piscicolidae), and praniza larvae of unidentified gnathiid isopod/s (Isopoda: Gnathiidae). Two of these species, B. plicobranchus and S. macrothela, are reported for the first time on G. typus. Only C. furcisetifer and S. macrothela were relatively common, encountered on 31% and 40% of G. typus, respectively. Gnathiids were observed infrequently, encountered on 13% of G. typus, and D. cairae and B. plicobranchus were scarce, encountered on 1% and 2% of G. typus, respectively. Intensity of infection for C. furcisetifer and gnathiids increased with host length. Likelihood of infection varied seasonally for C. furcisetifer, being considerably lower in summer, and regionally for gnathiids, being greatest at Shark Bay. Intensity and likelihood of infection for S. macrothela increased with host length and varied regionally, being greatest at Shark Bay., Conclusion: These findings improve our understanding of the downstream impacts for dependent parasites that might arise should populations of G. typus continue to decline., (© 2024. The Author(s).)

Stem cells are the fundamental drivers of growth during development and adult organ homeostasis. The properties that define stem cells - self-renewal and differentiation - are highly biosynthetically demanding. In order to fuel this demand, stem and progenitor cells engage in hypertranscription, a global amplification of the transcriptome. While standard normalization methods in transcriptomics typically mask hypertranscription, new approaches are beginning to reveal a remarkable range in global transcriptional output in stem and progenitor cells. We discuss technological advancements to probe global transcriptional shifts, review recent findings that contribute to defining hallmarks of stem cell hypertranscription, and propose future directions in this field., Competing Interests: Declaration of interests No interests are declared., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background/aims: Since the onset of the coronavirus disease 2019 (COVID-19) pandemic, 103.4 million cases and 1.1 million deaths have occurred nationally as of November 2023. Despite the benefit of mitigating measures, the pandemic's effect on participant safety is rarely documented., Methods: This study assessed noncompliance occurring from July 2019 to August 2021 that were stratified by the date of noncompliance (before or after restrictions). Events were described by size, site, noncompliance type, primary category, subcategory, and cause. In addition, noncompliance associated with COVID-19 was analyzed to determine characteristics., Results: In total, 323 noncompliance events occurred across 21,146 participants at risk in 35 protocols. The overall rate of noncompliance increased from 0.008 events per participant to 0.022 events per participant after the COVID-19 restrictions ( p  < 0.001). For onsite protocols, the median within protocol change in rates was 0.001 (interquartile range = 0.141) after the onset of COVID-19 restrictions ( p  = 0.54). For large-sized protocols ( n  ≥ 100), the median within protocol change in rates was also 0.001 (interquartile range = 0.017) after COVID-19 restrictions ( p  = 0.15). For events related to COVID-19 restrictions, 160/162 (99%) were minor deviations, 161/162 (99%) were procedural noncompliance, and 124/162 (77%) were an incomplete study visit., Conclusion: These noncompliance events have implications for clinical trial methodology because nonadherence to trial design can lead to participant safety concerns and loss of trial data validity. Protocols should be written to better facilitate the capture of all safety and efficacy data. This recommendation should be considered when changes occur to the protocol environment that are outside of the study team's control., Competing Interests: Declaration of conflicting interestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: L.R. is the incoming Vice Chair of the American Academy of Neurology’s Section on Experimental Neurotherapeutics and is the Intramural NINDS Program Director for the NINDS-FDA Fellowship in Clinical Trial Methodology and Regulatory Science.

This study reports the metazoan ectoparasite fauna of juvenile Critically Endangered green sawfish, Pristis zijsron, and sympatric elasmobranchs in Western Australia. Five parasite taxa were found on 76 screened P. zijsron: Caligus furcisetifer (Copepoda: Caligidae), Dermopristis pterophila (Monogenea: Microbothriidae), Branchellion plicobranchus and Stibarobdella macrothela (Hirudinea: Piscicolidae), and praniza larvae of an unidentified gnathiid isopod. Only C. furcisetifer and D. pterophila were common, exhibiting discrepant site-specificity, with C. furcisetifer occurring mostly on the head and rostrum, and D. pterophila around the pectoral and pelvic fins. Intensity of infection for C. furcisetifer and D. pterophila increased with host total length and was influenced by host sex, but in opposite directions; intensity of C. furcisetifer was greater on female P. zijsron, whereas intensity of D. pterophila was greater on males. In the Ashburton River, likelihood of infection for C. furcisetifer and D. pterophila on P. zijsron increased with time since substantial freshwater discharge events, suggesting decreased salinity impacts both taxa. In addition to P. zijsron, five other sympatric elasmobranch species were opportunistically screened for ectoparasites in the study area: the giant shovelnose ray, Glaucostegus typus, the eyebrow wedgefish, Rhynchobatus palpebratus, the nervous shark, Carcharhinus cautus, the lemon shark, Negaprion acutidens, and the graceful shark, Carcharhinus amblyrhynchoides. Caligus furcisetifer was found on R. palpebratus; no other parasites of P. zijsron were found on other sympatric elasmobranch species. Conversely, Perissopus dentatus (Copepoda: Pandaridae) was found on all three carcharhinids but not on batoid rays (P. zijsron, G. typus or R. palpebratus)., Competing Interests: Declaration of competing interest The authors declare there are no competing interests associated with this research., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Feeding habits of herbivorous fishes play an important role in shaping the form and function of coastal marine ecosystems. Rabbitfishes (Siganidae) are important consumers of macroalgae on Indo-West Pacific coral reefs. However, it is unclear how their diet varies among and within species at biogeographical scales, casting doubt on their precise functional roles across different regions. The present study assessed the inter- and intra-specific diet variation of four rabbitfishes ( Siganus trispilos, Siganus corallinus, Siganus virgatus and Siganus doliatus ) factored by morphological relatedness among populations from Ningaloo Reef (western Australia), the Great Barrier Reef (GBR, eastern Australia) and the Yaeyama Islands (Okinawa Prefecture, Japan). Results showed that the region had a strong effect on diet, effectively reducing the expected effect of morphologic similitude. While intra-specific differences were only significant when populations inhabited different regions; interspecific differences were not as predicted, with different morphotypes having similar diets when populations inhabited the same regions. Rabbitfishes consumed more corticated and filamentous macroalgae on the GBR, more foliose and membranous macroalgae at the Yaeyama Islands, and more leathery macroalgae at Ningaloo Reef. The findings indicate that rabbitfishes have high diet plasticity, and hence their functional role as mediators of competition between macroalgae and corals can change across biogeographic regions. Local context is therefore important when assessing the diet and functional role of herbivorous fishes. As climate change unfolds, shifts in the distribution, trophic behaviour and function of species are expected, making the study of trophic plasticity more important., Competing Interests: No conflicts of interest are declared by the authors., (© 2024 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)

Forficuloecus pezopori Martin, Keatley & Ash n. sp. from the western ground parrot Pezoporus flaviventris North, 1911 (Psittaculidae) is proposed based on combined evidence from morphology and COI mitochondrial DNA. Phylogenetically, the new species is closest to its two known congeners from Western Australia: F . josephi Price, Johnson & Palma, 2008 from Bourke's parrot Neopsephotus bourkii (Gould, 1841) and the scarlet-chested parrot Neophema splendida (Gould, 1841), and F . palmai Guimarães, 1985 from the Australian ringneck parrot Barnardius zonarius (Shaw, 1805). Morphologically it is distinguishable by abdominal chaetotaxy and characters of the male genitalia, and is most similar to F . josephi and F . greeni Guimarães, 1985; the latter has no representative sequence data. Forficuloecus pezopori is the eleventh species of its genus and the only metazoan parasite known from P . flaviventris , which is among Australia's most endangered vertebrates. The new louse is apparently restricted to P . flaviventris and is therefore co-endangered, facing at least the same likelihood of extinction as its host. We recommend ongoing translocation and field monitoring efforts for P . flaviventris include monitoring but not treatment for lice infestations in otherwise healthy individuals, and that the care management plan for captive P . flaviventris considers that F . pezopori is similarly imperilled., Competing Interests: The authors declare no conflict of interest., (© 2024 The Authors.)

Among children with multiple congenital melanocytic nevi, 25% have no established genetic cause, of whom many develop a hyperproliferative and severely pruritic phenotype resistant to treatment. Gene fusions have been reported in individual cases of congenital melanocytic nevi. We studied 169 patients with congenital melanocytic nevi in this study, 38 of whom were double wild type for pathogenic NRAS/BRAF variants. Nineteen of these 38 patients had sufficient tissue to undergo RNA sequencing, which revealed mosaic BRAF fusions in 11 of 19 patients and mosaic RAF1 fusions in 1 of 19. Recurrently, fusions involved the loss of the 5´ regulatory domain of BRAF or RAF1 but preserved the kinase domain. We validated all cases and detected the fusions in two separate nevi in 5 of 12 patients, confirming clonality. The absence of the fusion in blood in 8 of 12 patients indicated mosaicism. Primary culture of BRAF-fusion nevus cells from 3 of 12 patients demonstrated highly increased MAPK activation, despite only mildly increased BRAF expression, suggesting additional mechanisms of kinase activation. Trametinib quenched MAPK hyperactivation in vitro, and treatment of two patients caused rapid improvement in bulk tissue, improving bodily movement and reducing inflammation and severe pruritus. These findings offer a genetic diagnosis to an additional group of patients and trametinib as a treatment option for the severe associated phenotypes., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

Parasitological assessment of marine fishes at Sodwana Bay in the iSimangaliso Marine Protected Area on the east coast of South Africa revealed a new species of cryptogonimid trematode infecting the pyloric caeca of the Dory Snapper, Lutjanus fulviflamma (Forsskål) (Lutjanidae). The new species is morphologically consistent with the concept of the large genus Siphoderina Manter, 1934; its phylogenetic position within this genus was validated through molecular sequencing of the ITS2 and partial 28S ribosomal DNA sub-regions. We name this species Siphoderina nana n. sp. and comment on the current state of understanding for this genus of cryptogonimids., (© 2023. The Author(s).)

With one exception, the only known hemiurid trematodes that do not use teleost fishes as definitive hosts instead occur in marine elapid snakes. These comprise six species across four genera and three subfamilies, and so presumably indicate at least three independent invasions of marine snakes from teleost fishes. Here, one of these taxa, Tubulovesicula laticaudi Parukhin, 1969 (= T. orientalis Chattopadhyaya, 1970 n. syn.) is reported from Sri Lanka, collected from Shaw's sea snake Hydrophis curtus (Shaw) (Elapidae: Hydrophiinae: Hydrophinii), the annulated sea snake H. cyanocinctus Daudin and the yellow sea snake H. spiralis (Shaw) off Nayaru in the Bay of Bengal, and from H. spiralis in Portugal Bay, Gulf of Mannar. Novel molecular data, for COI mtDNA and ITS2 and 28S rDNA, are the first for a species of Tubulovesicula Yamaguti, 1934. Nominally, Tubulovesicula belongs in the Dinurinae Looss, 1907, but in phylogenetic analyses based on 28S rDNA, our sequences for T. laticaudi resolved relatively distant from that for representatives of Dinurus Looss, 1907, the type-genus, rendering the subfamily polyphyletic. Tubulovesicula laticaudi resolved closest to data for the type-species of the Plerurinae Gibson & Bray, 1979, but that subfamily is also polyphyletic. These findings lead us to re-evaluate an alternative classification considered by Gibson & Bray (1979). We propose restricting the Dinurinae for forms with a permanent sinus-organ (Dinurus, Ectenurus Looss, 1907; Erilepturus Woolcock, 1935; Paradinurus Vigueras, 1958; Qadriana Bilqees, 1971) and resurrect the Mecoderinae Skrjabin & Guschanskaja, 1954 for forms with a temporary sinus-organ (Mecoderus Manter, 1940, Allostomachicola Yamaguti, 1958, Stomachicola Yamaguti, 1934 and Tubulovesicula)., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

The soundscape of a given habitat is a product of its physical environment, human activity, and presence of soniferous marine life, which can be used to understand ecosystem processes, habitat quality, and biodiversity. Shallow coral habitats are hotspots of biodiversity and marine life. Deep-sea coral environments, in comparison, are generally poorly understood. Four soundscapes along the U.S. Outer Continental Shelf (OCS) and one soundscape from the Great Barrier Reef were quantified to explore how differences in habitat, depth, and substrate manifest acoustically. Comparisons were made between (1) deep, cold-water and shallow, warm-water coral reefs and (2) deep-sea coral and sandy bottom habitats. Application of the soundscape code to recordings in each location seeded cluster analyses of soundscape metrics and an assessment of daily trends to quantitatively compare the soundscapes. The shallow, tropical reef soundscape differed from the deep-sea soundscapes in amplitude and impulsiveness. Differences in soundscape properties among the deep-sea soundscapes suggested cold-water coral sites produce different soundscapes than the deep sites without live hard bottom. This initial assessment of deep-sea soundscapes along the U.S. OCS provides baseline acoustic properties in a region likely to experience changes due to climate and human use., (© 2023 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).)

Embryos across metazoan lineages can enter reversible states of developmental pausing, or diapause, in response to adverse environmental conditions. The molecular mechanisms that underlie this remarkable dormant state remain largely unknown. Here we show that N 6 -methyladenosine (m 6 A) RNA methylation by Mettl3 is required for developmental pausing in mouse blastocysts and embryonic stem (ES) cells. Mettl3 enforces transcriptional dormancy through two interconnected mechanisms: (1) it promotes global mRNA destabilization and (2) it suppresses global nascent transcription by destabilizing the mRNA of the transcriptional amplifier and oncogene N-Myc, which we identify as a crucial anti-pausing factor. Knockdown of N-Myc rescues pausing in Mettl3 -/- ES cells, and forced demethylation and stabilization of Mycn mRNA in paused wild-type ES cells largely recapitulates the transcriptional defects of Mettl3 -/- ES cells. These findings uncover Mettl3 as a key orchestrator of the crosstalk between transcriptomic and epitranscriptomic regulation during developmental pausing, with implications for dormancy in adult stem cells and cancer., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)

Phakomatosis pigmentovascularis is a diagnosis that denotes the coexistence of pigmentary and vascular birthmarks of specific types, accompanied by variable multisystem involvement, including CNS disease, asymmetrical growth, and a predisposition to malignancy. Using a tight phenotypic group and high-depth next-generation sequencing of affected tissues, we discover here clonal mosaic variants in gene PTPN11 encoding SHP2 phosphatase as a cause of phakomatosis pigmentovascularis type III or spilorosea. Within an individual, the same variant is found in distinct pigmentary and vascular birthmarks and is undetectable in blood. We go on to show that the same variants can cause either the pigmentary or vascular phenotypes alone, and drive melanoma development within pigmentary lesions. Protein structure modeling highlights that although variants lead to loss of function at the level of the phosphatase domain, resultant conformational changes promote longer ligand binding. In vitro modeling of the missense variants confirms downstream MAPK pathway overactivation and widespread disruption of human endothelial cell angiogenesis. Importantly, patients with PTPN11 mosaicism theoretically risk passing on the variant to their children as the germline RASopathy Noonan syndrome with lentigines. These findings improve our understanding of the pathogenesis and biology of nevus spilus and capillary malformation syndromes, paving the way for better clinical management., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Improving ventilation has been one of several COVID-19 prevention strategies implemented by kindergarten through grade 12 (K-12) schools to stay open for safe in-person learning. Because transmission of SARS-CoV-2 occurs through inhalation of infectious viral particles, it is important to reduce the concentration of and exposure time to infectious aerosols (1-3). CDC examined reported ventilation improvement strategies among U.S. K-12 public school districts using telephone survey data collected during August-December 2022. Maintaining continuous airflow through school buildings during active hours was the most frequently reported strategy by school districts (50.7%); 33.9% of school districts reported replacement or upgrade of heating, ventilation, and air conditioning (HVAC) systems; 28.0% reported installation or use of in-room air cleaners with high-efficiency particulate air (HEPA) filters; and 8.2% reported installation of ultraviolet (UV) germicidal irradiation (UVGI) devices, which use UV light to kill airborne pathogens, including bacteria and viruses. School districts in National Center for Education Statistics (NCES) city locales, the West U.S. Census Bureau region, and those designated by U.S. Census Bureau Small Area Income Poverty Estimates (SAIPE) as high-poverty districts reported the highest percentages of HVAC system upgrades and HEPA-filtered in-room air cleaner use, although 28%-60% of all responses were unknown or missing. Federal funding remains available to school districts to support ventilation improvements. Public health departments can encourage K-12 school officials to use available funding to improve ventilation and help reduce transmission of respiratory diseases in K-12 settings., Competing Interests: All authors have completed and submitted the International Committee of Medical Journal Editors form for disclosure of potential conflicts of interest. No potential conflicts of interest were disclosed.

An undescribed species of KannaphallusUnnithan, 1957 (Monogenoidea: Heteraxinidae) was collected from the gills of the golden trevally Gnathanodon speciosus (Forsskål) (Carangidae) from Moreton Bay, Queensland, during January 2016 and from Ningaloo Reef, Western Australia, during December 2021 and June 2022. The diagnosis for Kannaphallus was emended and the new species, Kannaphallus raphidium, was described. Kannaphallus virilis of Young, nec Unnithan was placed in synonymy with K. raphidium. The distal components of the male reproductive system and the arrangement of the clamp rows of the haptor occurred as mirror images among specimens of K. raphidium, suggesting that the respective antipodes of K. raphidium may have reproductive implications and function in the site selection of the parasite on the host's gills. A specimen of K. raphidium from Western Australia was sequenced for the cytochrome c oxidase subunit 1 (COI) mtDNA and ITS2 rDNA barcoding markers, and the phylogenetically informative 28S rDNA marker. Maximum likelihood and Bayesian inference analyses based on a partial 28S rDNA alignment, including all comparable heteraxinid sequence data available, resolved the Heteraxininae and Cemocotylinae as reciprocatively paraphyletic and provided evidence that Kannaphallus may be paraphyletic. No taxonomic changes concerning the subfamilies and genera of the Heteraxinidae were proposed. Finally, Kannaphallus univaginalisRamalingam, 1960 and Cemocotylelloides univaginalis (Ramalingam, 1960) Nitta, Kondo, Ohtsuka, Kamarudin, and Ismail, 2022 are considered nomen nuda sensu the International Code of Zoological Nomenclature., (© American Society of Parasitologists 2023.)

Performing reproducible vessel source level (SL) measurements is complicated by seabed reflections in shallow water. In deep water, with a hydrophone far from the seabed, it is straightforward to estimate propagation loss (PL) and convert sound pressure level (SPL) into SL using the method codified in the international standard ISO 17208-2 [International Organization for Standardization (ISO), Geneva, Switzerland (2019)]. Estimating PL is more difficult in shallow water because of the way that sound reflects from the seabed such that multiple propagation paths contribute to SPL. Obtaining reproducible SL measurements in shallow water requires straightforward and robust methods to estimate PL. From May to July 2021, a field experiment evaluated different methods of measuring vessel SL in shallow water. The same vessels were measured many times in water depths of 30, 70, and 180 m. In total, 12 079 SL measurements were obtained from 1880 vessel transits and 16 hydrophones, distributed across 3 moored vertical line arrays and 2 moored horizontal line arrays. The experiment confirmed that it is possible to obtain reproducible vessel SL estimates in shallow water comparable to within ±2.5 dB of ISO-compliant measurements in deep water and repeatable to within ±1.5 dB.

Many respiratory diseases, including COVID-19, can be spread by aerosols expelled by infected people when they cough, talk, sing, or exhale. Exposure to these aerosols indoors can be reduced by portable air filtration units (air cleaners). Homemade or Do-It-Yourself (DIY) air filtration units are a popular alternative to commercially produced devices, but performance data is limited. Our study used a speaker-audience model to examine the efficacy of two popular types of DIY air filtration units, the Corsi-Rosenthal cube and a modified Ford air filtration unit, in reducing exposure to simulated respiratory aerosols within a mock classroom. Experiments were conducted using four breathing simulators at different locations in the room, one acting as the respiratory aerosol source and three as recipients. Optical particle spectrometers monitored simulated respiratory aerosol particles (0.3-3 μm) as they dispersed throughout the room. Using two DIY cubes (in the front and back of the room) increased the air change rate as much as 12.4 over room ventilation, depending on filter thickness and fan airflow. Using multiple linear regression, each unit increase of air change reduced exposure by 10%. Increasing the number of filters, filter thickness, and fan airflow significantly enhanced the air change rate, which resulted in exposure reductions of up to 73%. Our results show DIY air filtration units can be an effective means of reducing aerosol exposure. However, they also show performance of DIY units can vary considerably depending upon their design, construction, and positioning, and users should be mindful of these limitations., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Objective: To describe parent-reported barriers and facilitators to reading to preterm-born infants, both in the neonatal intensive care unit (NICU) and at home. We hypothesized that families of preterm infants face unique barriers previously not described in the literature, and that understanding barriers from the parent perspective will uncover strategies for improving the NICU environment and cognitive home environment and allow refinement of current reading programs., Methods: We performed a qualitative study of parents of 0 to 12 month corrected age infants presenting to neonatal follow-up clinic. Infants were born <33 weeks gestational age or with birth weight ≤1500 grams. Parents completed a semistructured interview about reading practices. Answers to open-ended questions were coded to highlight key themes., Results: We reached thematic saturation after interviewing 23 parents of 28 infants. Parent-generated items about barriers and facilitators to reading in the NICU and at home were each coded into 4 themes. Competing responsibilities and believing that reading was developmentally inappropriate were 2 shared themes for barriers to reading in both the NICU and home environment. Support people and understanding developmental impact were 2 shared themes for facilitators to reading in both the NICU and home environment., Conclusions: This qualitative work elucidates new targets for novel programs intended to support cognitive development of high-risk preterm infants. Addressing the unique, parent-reported barriers that we have identified and supporting adoption of the facilitators could increase word exposure for preterm infants, starting in the NICU and continuing at home., (Copyright © 2022 Academic Pediatric Association. Published by Elsevier Inc. All rights reserved.)

A new cryptogonimid trematode, Siphoderina hustoni n. sp., is reported, collected off Lizard Island, Queensland, Australia, from the Maori snapper Lutjanus rivulatus (Cuvier). The new species is moderately distinctive within the genus. It is larger and more elongate than most other species of Siphoderina Manter, 1934, has the shortest forebody of any, a relatively large ventral sucker, a long post-testicular zone, and is perhaps most recognisable for the substantial space in the midbody between the ventral sucker and ovary devoid of uterine coils and vitelline follicles, the former being restricted to largely posterior to the ovary and the latter distributed from the level of the anterior testis to the level of the ovary. In phylogenetic analyses of 28S ribosomal DNA, the new species resolved with the other nine species of Siphoderina for which sequence data are available, all of which are from Queensland waters and from lutjanid and haemulid fishes. Molecular barcode data were also generated, for the ITS2 ribosomal DNA and cox1 mitochondrial DNA markers. The new species is the first cryptogonimid known from L. rivulatus and the first metazoan parasite reported from that fish in Australian waters., (© 2022. The Author(s).)

Vat photopolymerization (VP), a type of additive manufacturing process that cures resin to build objects, can emit potentially hazardous particles and gases. We evaluated two VP technologies, stereolithography (SLA) and digital light processing (DLP), in three separate environmental chambers to understand task-based impacts on indoor air quality. Airborne particles, total volatile organic compounds (TVOCs), and/or specific volatile organic compounds (VOCs) were monitored during each task to evaluate their exposure potential. Regardless of duration, all tasks released particles and organic gases, though concentrations varied between SLA and DLP processes and among tasks. Maximum particle concentrations reached 1200 #/cm 3 and some aerosols contained potentially hazardous elements such as barium, chromium, and manganese. TVOC concentrations were highest for the isopropyl alcohol (IPA) rinsing, soaking, and drying post-processing tasks (up to 36.8 mg/m 3 ), lowest for the resin pouring pre-printing, printing, and resin recovery post-printing tasks (up to 0.1 mg/m 3 ), and intermediate for the curing post-processing task (up to 3 mg/m 3 ). Individual VOCs included, among others, the potential occupational carcinogen acetaldehyde and the immune sensitizer 2-hydroxypropyl methacrylate (pouring, printing, recovery, and curing tasks). Careful consideration of all tasks is important for the development of strategies to minimize indoor air pollution and exposure potential from VP processes., Competing Interests: Conflicts of Interest: The authors declare no conflict of interest.

Effective COVID-19 prevention in kindergarten through grade 12 (K-12) schools requires multicomponent prevention strategies in school buildings and school-based transportation, including improving ventilation (1). Improved ventilation can reduce the concentration of infectious aerosols and duration of potential exposures (2,3), is linked to lower COVID-19 incidence (4), and can offer other health-related benefits (e.g., better measures of respiratory health, such as reduced allergy symptoms) (5). Whereas ambient wind currents effectively dissipate SARS-CoV-2 (the virus that causes COVID-19) outdoors,* ventilation systems provide protective airflow and filtration indoors (6). CDC examined reported ventilation improvement strategies among a nationally representative sample of K-12 public schools in the United States using wave 4 (February 14-March 27, 2022) data from the National School COVID-19 Prevention Study (NSCPS) (420 schools), a web-based survey administered to school-level administrators beginning in summer 2021. † The most frequently reported ventilation improvement strategies were lower-cost strategies, including relocating activities outdoors (73.6%), inspecting and validating existing heating, ventilation and air conditioning (HVAC) systems (70.5%), and opening doors (67.3%) or windows (67.2%) when safe to do so. A smaller proportion of schools reported more resource-intensive strategies such as replacing or upgrading HVAC systems (38.5%) or using high-efficiency particulate air (HEPA) filtration systems in classrooms (28.2%) or eating areas (29.8%). Rural and mid-poverty-level schools were less likely to report several resource-intensive strategies. For example, rural schools were less likely to use portable HEPA filtration systems in classrooms (15.6%) than were city (37.7%) and suburban schools (32.9%), and mid-poverty-level schools were less likely than were high-poverty-level schools to have replaced or upgraded HVAC systems (32.4% versus 48.8%). Substantial federal resources to improve ventilation in schools are available. § Ensuring their use might reduce SARS-CoV-2 transmission in schools. Focusing support on schools least likely to have resource-intensive ventilation strategies might facilitate equitable implementation of ventilation improvements., Competing Interests: All authors have completed and submitted the International Committee of Medical Journal Editors form for disclosure of potential conflicts of interest. Kenneth R. Mead and Stephen B. Martin, Jr. report multiple roles on technical and standard committees within ASHRAE. No other potential conflicts of interest were disclosed.

Exposure to elevated levels of diacetyl in flavoring and microwave popcorn production has been associated with respiratory impairment among workers including from a severe lung disease known as obliterative bronchiolitis. Laboratory studies demonstrate damage to the respiratory tract in rodents exposed to either diacetyl or the related alpha-diketone 2,3-pentanedione. Respiratory tract damage includes the development of obliterative bronchiolitis-like changes in the lungs of rats repeatedly inhaling either diacetyl or 2,3-pentanedione. In one flavored coffee processing facility, current workers who spent time in higher diacetyl and 2,3-pentanedione areas had lower lung function values, while five former flavoring room workers were diagnosed with obliterative bronchiolitis. In that and other coffee roasting and packaging facilities, grinding roasted coffee beans has been identified as contributing to elevated levels of diacetyl and 2,3-pentanedione. To reduce worker exposures, employers can take various actions to control exposures according to the hierarchy of controls. Because elimination or substitution is not applicable to coffee production facilities not using flavorings, use of engineering controls to control exposures at their source is especially important. This work demonstrates the use of temporary ventilated enclosures around grinding equipment in a single coffee roasting and packaging facility to mitigate diacetyl and 2,3-pentanedione emissions from grinding equipment to the main production space. Concentrations of diacetyl and 2,3-pentanedione were measured in various locations throughout the main production space as well as inside and outside of ventilated enclosures to evaluate the effect of the enclosures on exposures. Diacetyl and 2,3-pentanedione concentrations outside one grinder enclosure decreased by 95 and 92%, respectively, despite ground coffee production increasing by 12%, after the enclosure was installed. Outside a second enclosure, diacetyl and 2,3-pentanedione concentrations both decreased 84%, greater than the 33% decrease in ground coffee production after installation. Temporary ventilated enclosures used as engineering control measures in this study effectively reduced emissions of diacetyl and 2,3-pentanedione at the source in this facility. These findings motivated management to explore options with a grinding equipment manufacturer to permanently ventilate their grinders to reduce emissions of diacetyl and 2,3-pentanedione., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Stanton, McClelland, Beaty, Ranpara and Martin.)

Experimental research has shown that beaked whales exhibit strong avoidance reactions to naval active sonars used during antisubmarine warfare training exercises, including cessation of echolocation and foraging activity. Behavioural responses to sonar have also been linked to strandings and mortality. Much of the research on the responses of beaked whales and other cetaceans to naval active sonar has occurred on or near U.S. naval training ranges, and the impacts of sonar in other regions remain poorly understood, particularly as these impacts, including mortality, are likely to go unobserved in offshore areas. In September 2016 the multinational naval exercise 'CUTLASS FURY 2016' (CF16) was conducted off eastern Canada. We used passive acoustic recordings collected in the region to quantify the occurrence and characteristics of sonar signals, measure ambient noise levels, and assess changes in the acoustic activity of beaked and sperm whales. The number of hours per day with echolocation clicks from Cuvier's beaked whales and sperm whales were significantly reduced during CF16, compared to the pre-exercise period in 2016 (sperm whales) and to control data from 2015 (both species). Clicks from an unidentified Mesoplodont beaked whale species, sporadically detected prior to CF16, were absent during the exercise and for 7 days afterward. These results suggest that beaked and sperm whales ceased foraging in the vicinity of CF16 and likely avoided the affected area. Such disturbance may have energetic, health, and fitness consequences., (© 2022. The Author(s).)

A new microbothriid monogenean Dermopristis pterophilus n. sp. is described from the skin of the Critically Endangered green sawfish Pristis zijsron Bleeker, 1851 in the Ashburton River delta, northern Western Australia. Analyses of the 28S ribosomal DNA marker and the molecular barcoding markers Histone 3 and Elongation Factor 1 α confirmed position among the Microbothriidae, with close affinity to the only other sequenced representative of Dermopristis Kearn, Whittington and Evans-Groing, 2010. The new species is morphologically consistent with the concept of Dermopristis ; it has two testes, lacks a male copulatory organ and has a simple haptor. It is smaller than its two congeners D . paradoxus Kearn, Whittington and Evans-Gowing, 2010 and D . cairae Whittington and Kearn, 2011 and is most similar to the former, distinguished only in that it lacks the strong, transverse, parallel ridges on the ventral body surface that characterise that species. It is more easily distinguished from D . cairae , differing in body shape, possession of a seminal receptacle, and relative position and size of the haptor. It may further differ from both species by fine details of the gut diverticula, although these details are difficult to ascertain. Spermatophores were observed in the new species, similar to those previously reported for D . cairae . The new species exhibits site attachment preference: infections were greatest on and immediately adjacent to the host pelvic fins (including male reproductive organs, i.e. claspers), moderate in proximity to the dorsal and pectoral fins, few on the caudal fin and peduncle, and infrequently, isolated worms occurred elsewhere on the dorsal and ventral surfaces of the body. There was no incidence of infection on the head (including rostrum). We presume D . pterophilus is restricted to P . zijsron and thus likely faces the same threat of extinction., Competing Interests: The authors declare no conflict of interest associated with this research., (© 2022 The Authors.)

Fused filament fabrication three-dimensional (FFF 3-D) printing is thought to be environmentally sustainable; however, significant amounts of waste can be generated from this technology. One way to improve its sustainability is via distributed recycling of plastics in homes, schools, and libraries to create feedstock filament for printing. Risks from exposures incurred during recycling and reuse of plastics has not been incorporated into life cycle assessments. This study characterized contaminant releases from virgin (unextruded) and recycled plastics from filament production through FFF 3-D printing. Waste polylactic acid (PLA) and acrylonitrile butadiene styrene (ABS) plastics were recycled to create filament; virgin PLA, ABS, high and low density polyethylenes, high impact polystyrene, and polypropylene pellets were also extruded into filament. The release of particles and chemicals into school classrooms was evaluated using standard industrial hygiene methodologies. All tasks released particles that contained hazardous metals (e.g., manganese) and with size capable of depositing in the gas exchange region of the lung, i.e., granulation of waste PLA and ABS (667 to 714 nm) and filament making (608 to 711 nm) and FFF 3-D printing (616 to 731 nm) with waste and virgin plastics. All tasks released vapors, including respiratory irritants and potential carcinogens (benzene and formaldehyde), mucus membrane irritants (acetone, xylenes, ethylbenzene, and methyl methacrylate), and asthmagens (styrene, multiple carbonyl compounds). These data are useful for incorporating risks of exposure to hazardous contaminants in future life cycle evaluations to demonstrate the sustainability and circular economy potential of FFF 3-D printing in distributed spaces., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

The habitat of the endangered southern resident killer whale (SRKW) overlaps major international shipping lanes near the Port of Vancouver, British Columbia. Shipping is a dominant source of underwater noise, which can hinder SRKW key life functions. To reduce environmental pressure on the SRKWs, Vancouver Fraser Port Authority offers incentives for quieter ships. However, the absence of a widely accepted underwater radiated noise (URN) measurement procedure hinders the determination of relative quietness. We review URN measurement procedures, summarizing results to date from two Canadian-led projects aimed at improving harmonization of shallow-water URN measurement procedures: One supports the International Organization for Standardization (ISO) in the development of a URN measurement standard; the other supports the alignment of URN measurement procedures developed by ship classification societies. Weaknesses in conventional shallow-water URN metrics are identified, and two alternative metrics proposed. Optimal shallow-water measurement geometry is identified., (Copyright © 2021. Published by Elsevier Ltd.)

There is strong evidence associating the indoor environment with transmission of SARS-CoV-2, the virus that causes COVID-19. SARS-CoV-2 can spread by exposure to droplets and very fine aerosol particles from respiratory fluids that are released by infected persons. Layered mitigation strategies, including but not limited to maintaining physical distancing, adequate ventilation, universal masking, avoiding overcrowding, and vaccination, have shown to be effective in reducing the spread of SARS-CoV-2 within the indoor environment. Here, we examine the effect of mitigation strategies on reducing the risk of exposure to simulated respiratory aerosol particles within a classroom-style meeting room. To quantify exposure of uninfected individuals (Recipients), surrogate respiratory aerosol particles were generated by a breathing simulator with a headform (Source) that mimicked breath exhalations. Recipients, represented by three breathing simulators with manikin headforms, were placed in a meeting room and affixed with optical particle counters to measure 0.3-3 µm aerosol particles. Universal masking of all breathing simulators with a 3-ply cotton mask reduced aerosol exposure by 50% or more compared to scenarios with simulators unmasked. While evaluating the effect of Source placement, Recipients had the highest exposure at 0.9 m in a face-to-face orientation. Ventilation reduced exposure by approximately 5% per unit increase in air change per hour (ACH), irrespective of whether increases in ACH were by the HVAC system or portable HEPA air cleaners. The results demonstrate that mitigation strategies, such as universal masking and increasing ventilation, reduce personal exposure to respiratory aerosols within a meeting room. While universal masking remains a key component of a layered mitigation strategy of exposure reduction, increasing ventilation via system HVAC or portable HEPA air cleaners further reduces exposure.

We report eight species of Lintonium from tetraodontiform fishes from Australian waters and describe six of them as new. Two species are described from tetraodontids from the Great Barrier Reef (GBR): Lintonium kostadinovae n. sp. from Arothron nigropunctatus (Bloch & Schneider) and Arothron hispidus (Linnaeus); and Lintonium droneni n. sp. from A. nigropunctatus. Two species are described from temperate monacanthids: Lintonium crowcrofti n. sp. from Meuschenia hippocrepis (Quoy & Gaimard) and Meuschenia freycineti (Quoy & Gaimard) off Tasmania and from M. hippocrepis off Glenelg, South Australia and off Fremantle, Western Australia; and Lintonium blendi n. sp. from M. hippocrepis off Stanley, Tasmania. The final two new species are described from tropical monacanthids: Lintonium currani n. sp. from Cantherhines pardalis (Rüppell) from Ningaloo Reef, Western Australia; and Lintonium madhaviae n. sp. from Amanses scopas (Cuvier) from the southern GBR. Two previously described species are reported from tetraodontids: Lintonium pulchrum (Johnston, 1913) Yamaguti, 1954 from Arothron stellatus (Anonymous), A. hispidus, A. manilensis (Marion de Procé) and Lagocephalus lunaris (Bloch & Schneider) from the GBR and southern Queensland; and Lintonium consors (Lühe, 1906) Crowcroft, 1950 from A. nigropunctatus from the southern GBR. Sequence data for three markers (ITS2 and 28S rDNA and cox1 mtDNA) for six of the eight species (L. crowcrofti n. sp., L. currani n. sp., L. droneni n. sp., L. kostadinovae n. sp., L. madhaviae n. sp. and L. pulchrum) are the first for the genus and distinguish each species unambiguously. Many records of species of Lintonium, especially widespread records of the type species, L. vibex (Linton, 1900) Stunkard & Nigrelli, 1930, remain to be clarified. A key finding of the present study is that three fish species (A. hispidus, A. nigropunctatus and M. hippocrepis) are identified as harbouring either two or three species of Lintonium at individual localities., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)

Extrusion of high-melt-temperature polymers on large-format additive manufacturing (LFAM) machines releases particles and gases, though there is no data describing their physical and chemical characteristics. Emissions from two LFAM machines were monitored during extrusion of acrylonitrile butadiene styrene (ABS) and polycarbonate (PC) polymers as well as high-melt-temperature Ultem (poly(ether imide)), polysulfone (PSU), poly(ether sulfone) (PESU), and polyphenylene sulfide (PPS) polymers. Filter samples of particles were collected for quantification of elements and bisphenol A and S (BPA, BPS) and visualization of morphology. Individual gases were quantified on substance-specific media. Aerosol sampling demonstrated that concentrations of elements were generally low for all polymers, with a maximum of 1.6 mg/m 3 for iron during extrusion of Ultem. BPA, an endocrine disruptor, was released into air during extrusion of PC (range: 0.4 ± 0.1 to 21.3 ± 5.3 μ g/m 3 ). BPA and BPS (also an endocrine disruptor) were released into air during extrusion of PESU (BPA, 2.0-8.7 μ g/m 3 ; BPS, 0.03-0.07 μ g/m 3 ). Work surfaces and printed parts were contaminated with BPA (<8-587 ng/100 cm 2 ) and BPS (<0.22-2.5 ng/100 cm 2 ). Gas-phase sampling quantified low levels of respiratory irritants (phenol, SO 2 , toluene, xylenes), possible or known asthmagens (caprolactam, methyl methacrylate, 4-oxopentanal, styrene), and possible occupational carcinogens (benzene, formaldehyde, acetaldehyde) in air. Characteristics of particles and gases released by high-melt-temperature polymers during LFAM varied, which indicated the need for polymer-specific exposure and risk assessments. The presence of BPA and BPS on surfaces revealed a previously unrecognized source of dermal exposure for additive manufacturing workers using PC and PESU polymers., Competing Interests: The authors declare no competing financial interest.

SARS-CoV-2, the virus that causes COVID-19, can be spread by exposure to droplets and aerosols of respiratory fluids that are released by infected persons when they cough, sing, talk, or exhale. To reduce indoor transmission of SARS-CoV-2 between persons, CDC recommends measures including physical distancing, universal masking (the use of face masks in public places by everyone who is not fully vaccinated), and increased room ventilation (1). Ventilation systems can be supplemented with portable high efficiency particulate air (HEPA) cleaners* to reduce the number of infectious particles in the air and provide enhanced protection from transmission between persons (2); two recent reports found that HEPA air cleaners in classrooms could reduce overall aerosol particle concentrations by ≥80% within 30 minutes (3,4). To investigate the effectiveness of portable HEPA air cleaners and universal masking at reducing exposure to exhaled aerosol particles, the investigation team used respiratory simulators to mimic a person with COVID-19 and other, uninfected persons in a conference room. The addition of two HEPA air cleaners that met the Environmental Protection Agency (EPA)-recommended clean air delivery rate (CADR) (5) reduced overall exposure to simulated exhaled aerosol particles by up to 65% without universal masking. Without the HEPA air cleaners, universal masking reduced the combined mean aerosol concentration by 72%. The combination of the two HEPA air cleaners and universal masking reduced overall exposure by up to 90%. The HEPA air cleaners were most effective when they were close to the aerosol source. These findings suggest that portable HEPA air cleaners can reduce exposure to SARS-CoV-2 aerosols in indoor environments, with greater reductions in exposure occurring when used in combination with universal masking., Competing Interests: All authors have completed and submitted the International Committee of Medical Journal Editors form for disclosure of potential conflicts of interest. No potential conflicts of interest were disclosed.

Ultraviolet germicidal irradiation uses ultraviolet C (UV-C) energy to disinfect surfaces in clinical settings. Verifying that the doses of UV-C energy received by surfaces are adequate for proper disinfection levels can be difficult and expensive. Our study aimed to test commercially available colorimetric labels, sensitive to UV-C energy, and compare their precision with an accepted radiometric technique. The color-changing labels were found to predictably change color in a dose-dependent manner that would allow them to act as a qualitative alternative to radiometry when determining the minimum UV-C energy dosage received at surfaces. If deployed using careful protective techniques to avoid unintentional exposure to sunlight or other light sources, the use of colorimetric labels could provide inexpensive, easy, and accurate verification of effective UV-C dosing in clinical spaces., (Published by Oxford University Press on behalf of The British Occupational Hygiene Society 2021.)

Passive acoustic monitoring (PAM) can inform wildlife management by providing information on the distribution of cetaceans. This paper presents an automatic data selection for validation (ADSV) method to effectively identify all species acoustically present in large PAM data sets. The ADSV method involves the application of automated detectors, the automated selection of a portion of data for manual review, and the evaluation/optimization of automated detectors. Using an exemplar data set, results from the ADSV method were compared to a more intensive systematic manual review method. The two methods were found to have similar species occurrence results (hourly occurrence matching 73%-100%).

Background: Weight control behaviors (WCBs) typically involve appearance- or health-driven behaviors that may be influenced by physiological, psychological, or social factors. Sleep disturbances like insomnia are an important area of research for adolescent populations, as early intervention may result in improvements in other physical and mental health domains. The purpose of this study was to investigate the relationship of insomnia, psychosocial well-being, and current WCBs in healthy-weight female adolescents., Method: Female adolescents (N = 323; M age  = 12.33 ± .04) who had healthy body mass index (BMI) levels completed self-report items on insomnia, depression, self-esteem, and physical self-concept. Multivariate analysis of covariance (MANCOVA), controlling for age, was conducted to further examine differences in insomnia and psychosocial variables among the WCB groups., Results: Compared to those who were trying to stay the same weight or not trying to do anything about their weight, the girls who were trying to lose weight had significantly greater insomnia and depression symptoms, and lower self-esteem, with small to medium effect sizes., Conclusions: Clinicians working with adolescent girls should include assessments of WCBs in addition to measures of insomnia symptoms, even for adolescent girls within a normal BMI range, as these are common and frequently co-occurring phenomena. Additional research is needed to further disentangle these complicated relationships.

In an effort to minimize protocol noncompliance in neurological research studies that can potentially compromise patient safety, delay completion of the study, and result in premature termination and added costs, we determined the effect of investigator trainings and site initiation visits (SIVs) on the occurrence of noncompliance events. Results of protocol audits conducted at the National Institute of Neurological Disorders and Stroke from 2003 to 2019 on 97 research protocols were retrospectively analyzed. Based on the depth of auditing and provision of investigator research training, audit data were separated into four arms: 1) Early Period, 2003 to 2012; 2) Middle Period, 2013 to 2016; and Late Period, 2017 to 2019, further divided into 3) Late Period without SIVs; and 4) Late Period with SIVs. Events of noncompliance were classified by the type of protocol deviation, the category, and the cause. In total, 952 events occurred across 1080 participants. Protocols audited during the Middle Period, compared to the Early Period, showed a decrease in the percentage of protocols with at least 1 noncompliance event. Protocols with SIVs had a further decrease in major, minor, procedural, eligibility, and policy events. Additionally, protocols audited during the Early Period had on average 0.46 major deviations per participant, compared to 0.26 events in protocols audited during the Middle Period, and 0.08 events in protocols audited during the Late Period with SIVs. Protocol deviations and noncompliance events in neurological clinical trials can be reduced by targeted investigator trainings and SIVs. These measures have major impacts on the integrity, safety, and effectiveness of human subjects research in neurology., (© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.)

The literature on emissions during material extrusion additive manufacturing with 3-D printers is expanding; however, there is a paucity of data for large-format additive manufacturing (LFAM) machines that can extrude high-melt-temperature polymers. Emissions from two LFAM machines were monitored during extrusion of six polymers: acrylonitrile butadiene styrene (ABS), polycarbonate (PC), high-melt-temperature polysulfone (PSU), poly(ether sulfone) (PESU), polyphenylene sulfide (PPS), and Ultem (poly(ether imide)). Particle number, total volatile organic compound (TVOC), carbon monoxide (CO), and carbon dioxide (CO 2 ) concentrations were monitored in real-time. Particle emission rate values (no./min) were as follows: ABS (1.7 × 10 11 to 7.7 × 10 13 ), PC (5.2 × 10 11 to 3.6 × 10 13 ), Ultem (5.7 × 10 12 to 3.1 × 10 13 ), PPS (4.6 × 10 11 to 6.2 × 10 12 ), PSU (1.5 × 10 12 to 3.4 × 10 13 ), and PESU (2.0 to 5.0 × 10 13 ). For print jobs where the mass of extruded polymer was known, particle yield values (g -1 extruded) were as follows: ABS (4.5 × 10 8 to 2.9 × 10 11 ), PC (1.0 × 10 9 to 1.7 × 10 11 ), PSU (5.1 × 10 9 to 1.2 × 10 11 ), and PESU (0.8 × 10 11 to 1.7 × 10 11 ). TVOC emission yields ranged from 0.005 mg/g extruded (PESU) to 0.7 mg/g extruded (ABS). The use of wall-mounted exhaust ventilation fans was insufficient to completely remove airborne particulate and TVOC from the print room. Real-time CO monitoring was not a useful marker of particulate and TVOC emission profiles for Ultem, PPS, or PSU. Average CO 2 and particle concentrations were moderately correlated ( r s = 0.76) for PC polymer. Extrusion of ABS, PC, and four high-melt-temperature polymers by LFAM machines released particulate and TVOC at levels that could warrant consideration of engineering controls. LFAM particle emission yields for some polymers were similar to those of common desktop-scale 3-D printers., Competing Interests: The authors declare no competing financial interest.

When evaluating pediatric patients with hyperpigmented lesions on the plantar aspect of the feet, the differential diagnosis should include talon noir, acral melanoma, and verruca with thrombosis. Talon noir are asymptomatic brown-black pigmented macules formed by intraepidermal hemorrhages of the feet caused by shear-force injury. Spontaneous resolution of talon noir typically occurs within 2-3 weeks. Clinicians should ensure that their patients who participate in sports have appropriately fitting shoes, which may prevent these lesions from occurring. This case report highlights a unique case of talon noir occurring in a young baseball player with an atypical distribution of plantar macules., (Copyright © 2020 National Association of Pediatric Nurse Practitioners. Published by Elsevier Inc. All rights reserved.)