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The present study aimed to tackle research gaps regarding how infrared heating affected macro- and micronutrients of lentil flours from seeds varying in size. Infrared treatments reduced resistant starch contents of lentil flours from 26.1-33.6% to 6.0-17.8%, increased protein digestibility from 73.6-75.0% to 78.2-82.2%, and enhanced soluble dietary fiber contents from 6.1-7.8% to 7.4-10.3%. Infrared treatments did not alter the primary limiting amino acid of Greenstar and Imvincible lentil flours (tryptophan) but changed that of Maxim to methionine + cysteine at 150 °C heating. Regarding micronutrients, the thermal modifications decreased the levels of heat-labile B vitamins, including B

Whole-grain wheat, in particular coloured varieties, may have health benefits in adults with chronic metabolic disease risk factors. Twenty-nine overweight and obese adults with chronic inflammation (high-sensitivity C-reactive protein) > 1·0 mg/l) replaced four daily servings of refined grain food products with bran-enriched purple or regular whole-wheat convenience bars (approximately 41–45 g fibre, daily) for 8 weeks in a randomised, single-blind parallel-arm study where body weight was maintained. Anthropometrics, blood markers of inflammation, oxidative stress, and lipaemia and metabolites of anthocyanins and phenolic acids were compared at days 1, 29 and 57 using repeated-measures ANOVA within groups and ANCOVA between groups at day 57, with day 1 as a covariate. A significant reduction in IL-6 and increase in adiponectin were observed within the purple wheat (PW) group. TNF-α was lowered in both groups and ferulic acid concentration increased in the regular wheat (RW) group. Comparing between wheats, only plasma TNF-α and glucose differed significantly (P < 0·05), that is, TNF-α and glucose decreased with RW and PW, respectively. Consumption of PW or RW products showed potential to improve plasma markers of inflammation and oxidative stress in participants with evidence of chronic inflammation, with modest differences observed based on type of wheat.

Lentils are an important member of the nutritious Leguminous crops, and the functional properties of lentil flours can be effectively improved by infrared heating, an efficient and short-time thermal processing method. This research primarily focused on the effects of tempering time (24–96 h) and seed size on the modification of lentils using infrared heating. Lentil seeds of three varieties, including CDC Greenstar (large green), CDC Imvincible (small green), and CDC Maxim (small red), were tempered at 25% moisture for 24, 48 and 96 h and then infrared heated to a surface temperature of 130 and 150 °C. Overall, under the same infrared heating treatment, a longer tempering period and a smaller seed size led to greater degrees of starch gelatinization and protein denaturation. In addition, a smaller seed size and a higher surface temperature tended to cause a higher level of photodegradation of amylose (possibly amylopectin too). Due to these physicochemical changes, the combined treatment of tempering and infrared heating noticeably reduced the average particle sizes, enhanced the water-holding capacity, diminished the peak and final viscosities, and decreased the gel hardness of the processed lentil flours. Generally, more obvious effects were found with higher levels of starch gelatinization, protein denaturation, and breakdown of amylose. The present study advanced our understanding of how extended tempering and seed size influenced the techno-functional properties of lentil flours modified using infrared heating. The new findings from the research are meaningful for the utilization of infrared heating to process lentil seeds for the development of novel food ingredients.

Summary Five legume seeds (kidney beans, green peas, black beans, lentil and pinto beans) were heated by infrared to a surface temperature of 140 °C. The changes in chemical composition, physical, mechanical and functional properties of the processed seeds were measured and compared to those of the raw seeds. Significant changes in the properties of the seeds in terms of increased volume, lower rupture point and toughness, higher water uptake and higher leaching losses (when the seeds were soaked in water) were obtained. The changes in the physical and mechanical properties were attributed to possible cracking of the seed. Even though trypsin inhibitor activity was reduced, infrared heating did not significantly affect the starch and protein components of the seeds. The functional characteristics of flour from the infrared-heated seeds were superior to those of flour from untreated seeds.

Review on FAU (Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed), with data on DNA, on the protein encoded, and where the gene is implicated.

The identification of the most suitable molecular targets for gene and drug therapy is the crucial first step in the development of new disease treatments. The rational identification of such targets depends on a detailed understanding of the pathological changes occuring at the molecular level. We have applied forward genetics approaches to the identification of the critical genes involved in the control of apoptosis in mammalian cells, since defective control of apoptosis underlies many diseases, including cancer and neurodegenerative diseases. We have identified two groups of genes by their effects on cell survival using retroviral cDNA functional expression cloning and retroviral insertional mutagenesis. The identification of these novel genes opens up new areas for apoptosis research and subsequently for the development of new gene and drug therapies.

Objective In view of recent media attention concerning the high level of alcohol and drug use reported in a group of newly qualified junior doctors, the aim of the present study was to assess the alcohol and drug habits of a group of current medical students. Methods Information about alcohol and illicit drug use was obtained from 136 second-year medical students (46 men, 90 women) at the University of Leeds by means of a personally administered questionnaire. Levels of anxiety and depression were also assessed. Results 86% of the students drank alcohol and among those who drank, a high proportion (52.6% of the men and 50.6% of the women), exceeded the recommended weekly limit of alcohol consumption of 21 units for men and 14 units women per week). Illicit drug use was reported by 33.1% of students (28.3% of men, 35.6% of women). The drug most commonly used was cannabis. According to the Hospital Anxiety and Depression (HAD) scale, 41.2% of the students (37% of men, 43.3% of women) had anxiety ratings within the clinically significant range (HAD scale> 8); 9.5% of students demonstrated clinically significant levels of depression (HAD scale> 8). However, these high levels of anxiety and depression did not correlate with high levels of alcohol consumption or drug use.

To date the response rates to biomodulated 5-fluorouracil in patients with metastatic or unresectable colorectal carcinoma have been varied. Potentially responsive patients are difficult to identify and treatment schedules are both expensive and toxic. Thus, any method that could be used to predict patient response would be both clinically and economically valuable. Increased p53 protein levels have previously been shown to correlate with disease progression in a series of colorectal carcinoma patients treated with 5-FU/folinic acid biomodulated chemotherapy. In addition to mutation of the p53 tumour suppressor gene, mutation of the K-ras gene at codon 12 has also been shown to be a frequent occurrence in the step-wise progression from normal colonic mucosa to adenocarcinoma. Oncogenic activity in the ras family has recently been shown to correlate with decreased levels of apoptosis and thus increased resistance to both radiation and certain chemotherapeutic agents. The aim of the present study was therefore to investigate if a correlation existed between mutation of the K-ras gene at codon 12 and disease progression in the series of colorectal carcinoma patients previously evaluated for levels of p53 protein expression. Response to 5-FU/folinic acid was assessed radiologically by CAT scan (WHO criteria) and clinically by Karnofsky performance scale (KPS) 3 months after the initial treatment. The presence of a K-ras gene mutation was assessed with radiolabelled oligonucleotide probes on amplified patient DNA, dot blotted on to a nylon membrane. Fifty-two patients were assessed and 25% were found to possess mutations at codon 12 of their K-ras gene. In contrast to increased levels of p53 protein, K-ras mutation at codon 12 did not correlate with disease progression when assessed either radiologically or clinically.

Biomodulated 5-flourouracil (5-FU) chemotherapy may limit disease progression in up to 50% of patients with metastatic or unresectable carcinoma of the colorectum. However, treatment is expensive and may be toxic. Thus any predictors of response may be clinically and economically valuable. The p53 gene is mutated in more than 50% of colorectal tumours, usually resulting in p53 overexpression. It may regulate cell cycle progression and cellular response to DNA damage. The principal anticancer activity of 5-FU is due to its ability to induce DNA damage. Fifty-nine patients received bolus intravenous 5-FU/folinic acid over 3 months. Response was assessed by CAT scan (WHO criteria). p53 protein overexpression was determined immunohistochemically from paraffin sections of the original primary tumour and resected metastases. Tumour over expression of p53 protein was associated with a lower rate of response and a higher rate of deterioration both radiologically (P0.03) and clinically (P0.05, chi 2 test for trend), but did not predict survival from start of treatment. Response was unrelated to age, sex, tumour grade, site of disease or chemotherapy schedule. Tumour p53 protein overexpression alone cannot be used to select advanced colorectal cancer patients for chemotherapy but may be useful in association with other markers of tumour biology.

Alan Woodall and Mark Pickard discuss education and career choices for medics who aren't just straight from the sixth form Almost 5% of British medical students are mature students, most of whom are graduates. These range from students entering immediately after a degree to those who have already pursued other careers. Compared with A level entrants, mature students in Britain have a lower dropout rate,1 better preclinical academic performance,2 and find it easier to communicate with patients in clinical years.3 According to Peter Richards, the former dean of St Mary's medical school, mature students “make a significant contribution to the stability and maturity of their immediate year and more widely in the medical school.”3 They often provide advice to younger students with problems who are reluctant to approach staff. In addition, some graduates with research backgrounds undertake research alongside their medical studies to the benefit of their school. It is difficult to enter medicine as a mature student in Britain, as the majority of places are for A level entrants. Few applicants over the age of 30 are admitted, on the grounds that they have less time to serve the NHS. Similar arguments were once used to limit the entry of women. Some medical schools interview all mature entrants to assess their commitment, while offering A level entrants places solely from their UCAS form, and yet mature students often have a greater awareness of what constitutes a career in medicine.4 Today many people pursue multiple careers and use previous life experience to enhance their current profession. The current recruitment policy on mature students appears outdated and based on subjective cost-benefit “analysis.” Few …

For a number of years, the consensus view amongst endocrinologists has been that thyroid hormones of maternal origin neither cross the placenta in significant amount1, nor are implicated in the development of the fetus in general, or of the fetal CNS in particular2. Nevertheless, for a number of reasons (some of which have been discussed in3), the validity of this view appeared to us to be open to doubt; we therefore initiated studies (ca 1980) specifically intended to verify or disprove some of the experimental evidence and theoretical postulates on which this view was based.