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There are few side-by-side comparisons on how Kentucky bluegrass (Poa pratensis L.) invasion has affected plant diversity and carbon (C) and nitrogen (N) dynamics. Between 2013 and 2015, four plots (4.9 × 4.9 m) were annually located on a long-term (1916−2016), moderately stocked pasture at the Northern Great Plains Research Laboratory (USDA-ARS) near Mandan, North Dakota. All plots were located on loamy ecological with minimal microsite differences. Each site had areas that were visually observed to have the largest vegetative component dominated by either Kentucky bluegrass (KBG) or dominated by other species (CONT). Two 1/8 m2 quadrats were clipped by species, including detached plant material (litter), within each subplot between mid to late July 2013, 2014, and 2015. This period represents peak biomass production and offers the best opportunity to capture cool- and warm-season perennial species. In 2013, soil samples were taken at depths of 0−5, 5−10, and 10−20 cm within each plot and thatch samples were hand excavated after clipping. Carbon and N were determined on biomass, litter, thatch, and soil samples. KBG plots produced 28% more biomass annually than the CONT plots. In 2014 and 2015, species richness was 0.7−2.1 × greater in the CONT plots. Although N and C concentrations were similar between treatments for aboveground biomass, litter, and thatch when expressed on a g m−2 basis, N was 34% greater for aboveground biomass and 600% greater for litter in KBG plots. Soil organic C and total soil N at 0−5 cm were 26% and 22% greater in KBG plots, respectively. Our findings indicate that KBG plots had less diversity and greater aboveground and belowground N and C than CONT. More follow-on research is needed to understand how C and N stocks and dynamics influence Kentucky bluegrass invasion.

Interest in cover crops is increasing but information is limited on integrating them into crop rotations especially in the relatively short growing season on the northern Great Plains. A 3-yr research project, initiated in 2009 near Mandan, North Dakota, USA, evaluated (1) what impact cover crops may have on subsequent cash crops yields and (2) whether cover crop mixtures are more productive and provide additional benefits compared to cover crop monocultures. The study evaluated 18 different cover crop monocultures and mixtures that were seeded in August following dry pea (Pisum sativum L.). The following year, spring wheat (Triticum aestivum L.), corn (Zea mays L.), soybean (Glycine max L.) and field pea were seeded into the different cover crop treatments and a non-treated control. A lack of timely precipitation in 2009 resulted in a low cover crop yield of 17 g m2 compared to 100 and 77 g m2 in 2008 and 2010, respectively. Subsequent cash crop yield was not affected by late-seeded cover crops. Cool-season cover crop monocultures were more productive than warm-season monocultures and some mixtures in 2008 and 2010. Relative yield total did not differ from one in any cover crop mixture suggesting that overyielding did not occur. Species selection rather than species diversity was the most important contributor to cover crop yield. Cover crops can be grown following short-season cash crops in the northern Great Plains, but precipitation timing and species selection are critical.

Coal surface mining in the northern Great Plains of the United States often produces mining spoils with physical and chemical barriers to successful revegetation, and this has resulted in experiments on reclamation with salvaged soil materials. There is a need to determine changes in soil properties and plant community and productivity decades after reclamation. Experiments were initiated in the mid-1970s by placing wedge-shaped masses of Haplustoll soils over leveled, saline-sodic mine spoils forming 2−5% hillslopes approximately 50 m long at two sites near Zap and Stanton, North Dakota. Seeding treatments at Zap included alfalfa (Medicago sativa L.), crested wheatgrass (CWG, Agropyron cristatum [L.] Gaertn.), smooth bromegrass (SBG, Bromus inermis Leyss.), and Russian wildrye (Psathyrostachy juncea [Fisch.] Nevski); at Stanton, native grasses (two Bouteloua spp.) replaced P. juncea. We examined soil, plant community, and productivity changes 3 decades after the start of the experiments. Leaching of soluble salts in subsoils and mine spoils improved soil quality (SQ). SQ improved more in lower slope areas because more rootzone was occupied by low SQ mine spoil. Initial forage yield patterns showed dependency on hillslope position, reflecting both soil depth effects in lower slope parts of hillslopes and apparent water redistribution effects in middle and upper slope areas. Evidence of SQ improvement over time was inferred by substantial decreases in yield dependency on hillslope position at both sites. The Zap site was grazed more heavily than Stanton, and species composition at Zap was 46% cool-season grasses (CWG greatest) and 40% broadleaves. Stanton had 87% cool-season grasses (SBG greatest) with 3% broadleaves. Leaching of salts was threefold greater at Zap and may have resulted from an abundance of deep-rooted broadleaves. Reclaiming mined land with salvaged soil and revegetation can improve SQ of mine spoil, which our results infer was driven by root growth and establishment of macroporosity.

In semiarid dryland farming regions, No-till and intensified crop rotations may improve water use. Our objective was to determine water use efficiency (WUE) and precipitation use efficiency (PUE) for spring wheat (Triticum aestivum L.; SW) grown in crop sequences representing different levels of intensification, under minimum (Min-till) or No-till. Root zone (0–122 cm) water at planting (SWP) was 2.76 cm higher for two- and three-phase sequences under Min-till than continuous spring wheat (CSW), and sequences with fallow averaged 2.65 cm higher than those without. Conversely, SWP was unaffected by sequence under No-till, with less effect of fallow. Root zone water at harvest was unaffected by the number of crop phases or fallow under Min-till. However, values for CSW exceeded the two- or three-phase sequences by 1.57 and 2.53 cm under No-till, and sequences without fallow averaged 1.52 cm more than those with fallow. The highest WUE (8.4 kg grain ha⁻¹ mm⁻¹ water) was observed when SW was grown in three-phase sequences compared with 7.7 kg grain ha⁻¹ mm⁻¹ water, for CSW and SW-fallow. Spring wheat under Min-till had higher WUE in sequences with fallow, but no effects of fallow occurred with No-till. The lowest PUE (near 3 kg grain ha⁻¹ mm⁻¹ precipitation) and no effects of tillage were found in sequences with fallow. However, SW in continuously cropped sequences had PUE between 5.5 and 6.4 kg grain ha⁻¹ mm⁻¹ precipitation and was higher under No-till. Both WUE and PUE are affected by intensification but may also be influenced by patterns of weather.

A global, unified dataset on Soil Organic Carbon (SOC) changes under perennial crops has not existed till now. We present a global, harmonised database on SOC change resulting from perennial crop cultivation. It contains information about 1605 paired-comparison empirical values (some of which are aggregated data) from 180 different peer-reviewed studies, 709 sites, on 58 different perennial crop types, from 32 countries in temperate, tropical and boreal areas; including species used for food, bioenergy and bio-products. The database also contains information on climate, soil characteristics, management and topography. This is the first such global compilation and will act as a baseline for SOC changes in perennial crops. It will be key to supporting global modelling of land use and carbon cycle feedbacks, and supporting agricultural policy development., Design Type(s)data integration objective • data collection and processing objective • factorial designMeasurement Type(s)amount of carbon atom in soilTechnology Type(s)digital curationFactor Type(s)climate • Species • experimental condition • ageSample Characteristic(s)Brazil • cultivated environment • Canada • United States of America • New Zealand • Italy • Germany • Cameroon • India • Sri Lanka • Venezuela • Kingdom of Denmark • Republic of Ireland • French Republic • United Kingdom • Kingdom of Spain • Costa Rica • Ethiopia • Ghana • Indonesia • Mexico • Nigeria • Republic of South Africa • Portuguese Republic • China • Malaysia • Turkey • Israel • Sweden • italy • Georgia • Puerto Rico • Tanzania • Australia Machine-accessible metadata file describing the reported data (ISA-Tab format)

Many integrated crop-livestock system (ICL) studies have been conducted, but few report cattle performance and forage nutrients. Here, we report one year of cattle weight gains for Angus yearling heifers and steers grazing ICL plots (ICL) or tame grass pastures (PAS) in four replicates. Five yearlings (avg. BW 390 ± 26 kg) were randomly assigned to graze each plot while provided ad libitum water and trace mineral salt. Cattle assigned to ICL grazed three crop rotations: 1) corn interseeded with soybean (C/S), 2) spring wheat interseeded with cover crop and 3) cover crop planted into spring wheat from the previous year. Yearlings in ICL were stocked first on green C/S, grazing an average of 60 days. Beginning C/S biomass was 1,827 ± 638 g m−2, yearling end BW was 465 ± 34 kg and ADG 1.28 ± 0.27 kg. Yearlings then grazed residual cover crop growth, averaging additional 42 d grazing while supplemented with a ration of baled cover crop forage, wheat straw, and corn. Ration intake averaged 15.35 ±0.6 kg animal-1 d-1. Final BW of ICL yearlings on cover crops averaged 499 ± 34 kg, ADG of 0.79 ± 0.29 kg. Yearlings assigned PAS were provided 1,911 ± 162 g m−2 at the beginning of grazing for 69 days, had final BW 443 ± 35 kg, ADG of 1.01 ± 0.22. Forage nutrient analyses are reported in Table 1, showing that individual crops are similar in ADF and TDN, but vary in crude protein content. Performance of ICL cattle exceeded that of PAS while on corn and soybean but decreased ICL performance while grazing cover crop was likely due to extreme cold weather at the time of grazing. Nutrient values reported here can be used to plan and predict cattle growth while grazing crop residues instead of pastures.

Recent interest in integrated crop-livestock (ICL) systems has prompted numerous investigations to quantify ecosystem service tradeoffs associated with management. However, few investigations have quantified ICL management effects on net global warming potential (GWP), particularly in semiarid regions. Therefore, we determined net GWP for grazed and ungrazed cropland in a long-term ICL study near Mandan, ND USA. Factors evaluated for their contribution to net GWP included carbon dioxide (CO2) emissions associated with production inputs and field operations, methane (CH4) emissions from enteric fermentation by beef cattle, change in soil carbon stocks, and soil-atmosphere CH4 and nitrous oxide (N2O) fluxes. Net GWP was significantly greater for grazed cropland (946 kg CO2equiv. ha-1 yr-1) compared to ungrazed cropland (200 kg CO2equiv. ha-1 yr-1) (P=0.0331). The difference in net GWP between treatments was largely driven by emissions from enteric fermentation (602 kg CO2equiv. ha-1 yr-1). Among other contributing factors, CO2 emissions associated with seed production and field operations were lower under ungrazed cropland (P = 0.0015 and 0.0135, respectively), while soil CH4 uptake was greater under grazed cropland (P = 0.0102). Soil-atmosphere N2O flux from each system negated nearly all the CO2equiv. sink capacity accrued from soil carbon stock change. As both production systems resulted in net greenhouse gas (GHG) emissions to the atmosphere, novel practices that constrain GHG sources and boost GHG sinks under semiarid conditions are recommended.

Funding Information: This modeling study was a joint effort of the Models4Pastures project within the framework of FACCE-JPI. Lutz Merbold and Kathrin Fuchs acknowledge funding received for the Swiss contribution to Models4Pastures (FACCE-JPI project, SNSF funded contract: 40FA40_154245/1) and for the Doc. Mobility fellowship (SNSF funded project: P1EZP2_172121). Lutz Merbold further acknowledges the support received for CGIAR Fund Council, Australia (ACIAR), Irish Aid, the European Union, the Netherlands, New Zealand, Switzerland, UK, USAID, and Thailand for funding to the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS) as well as for the CGIAR Research Program on Livestock. The NZ contributors acknowledge funding from the New Zealand Government Ministry of Primary Industries to support the aims of the Livestock Research Group of the Global Research Alliance on Agricultural Greenhouse Gases and from AgResearch's Strategic Science Investment Fund (the Forages for Reduced Nitrate Leaching (FRNL) research program). The UK partners acknowledge funding by DEFRA and the RCUK projects: N-Circle (BB/N013484/1), UGRASS (NE/M016900/1), and GREENHOUSE (NE/K002589/1). R.M. Rees and C.F.E. Topp also received funding from the Scottish Government Strategic Research Programme. Lorenzo Brilli, Camilla Dibari, and Marco Bindi received funding from the Italian Ministry of Agricultural Food and Forestry Policies (MiPAAF). The FR partners acknowledge funding from CN-MIP project funded by the French National Research Agency (ANR-13-JFAC-0001) and from ADEME (no. 12-60-C0023). Open access funding enabled and organized by Projekt DEAL Funding Information: This modeling study was a joint effort of the Models4Pastures project within the framework of FACCE‐JPI. Lutz Merbold and Kathrin Fuchs acknowledge funding received for the Swiss contribution to Models4Pastures (FACCE‐JPI project, SNSF funded contract: 40FA40_154245/1) and for the Doc. Mobility fellowship (SNSF funded project: P1EZP2_172121). Lutz Merbold further acknowledges the support received for CGIAR Fund Council, Australia (ACIAR), Irish Aid, the European Union, the Netherlands, New Zealand, Switzerland, UK, USAID, and Thailand for funding to the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS) as well as for the CGIAR Research Program on Livestock. The NZ contributors acknowledge funding from the New Zealand Government Ministry of Primary Industries to support the aims of the Livestock Research Group of the Global Research Alliance on Agricultural Greenhouse Gases and from AgResearch's Strategic Science Investment Fund (the Forages for Reduced Nitrate Leaching (FRNL) research program). The UK partners acknowledge funding by DEFRA and the RCUK projects: N‐Circle (BB/N013484/1), UGRASS (NE/M016900/1), and GREENHOUSE (NE/K002589/1). R.M. Rees and C.F.E. Topp also received funding from the Scottish Government Strategic Research Programme. Lorenzo Brilli, Camilla Dibari, and Marco Bindi received funding from the Italian Ministry of Agricultural Food and Forestry Policies (MiPAAF). The FR partners acknowledge funding from CN‐MIP project funded by the French National Research Agency (ANR‐13‐JFAC‐0001) and from ADEME (no. 12‐60‐C0023). Open access funding enabled and organized by Projekt DEAL Publisher Copyright: ©2020. The Authors. Open access funding enabled and organized by Projekt DEAL

This study evaluates the dynamics of soil organic carbon (SOC) underperennial crops across the globe. It quantifies the effect of change fromannual to perennial crops and the subsequent temporal changes in SOCstocks during the perennial crop cycle. It also presents an empiricalmodel to estimate changes in the SOC content under crops as a functionof time, land use, and site characteristics. We used a harmonised globaldataset containing paired-comparison empirical values of SOC andincluding different types of perennial crops (perennial grasses, palms,and woody plants) with different end-uses: bioenergy, food, other bioproducts, and short rotation coppice crops. Salient outcomes include: a20-year period encompassing a change from annual to perennial cropsled to an average 20% increase in SOC at 0-30 cm (6.0 ± 4.6 Mg ha-1gain) and a total of 10% increase over the 0-100 cm soil profile (5.7 ±10.9 Mg ha-1). A change from natural pasture to perennial cropdecreased SOC stocks by 1% over 0-30 cm (-2.5 ± 4.2 Mg ha-1) and10% over 0-100 cm (-13.6 ± 8.9 Mg ha-1). The effect of a land usechange from forest to perennial crops did not have significant impacts,probably due to the limited number of plots; but the data indicated thatwhile a 2% increase in SOC was observed at 0-30 cm (16.81 ± 55.1 Mgha-1), a decrease of 24% was observed at 30-100 cm (-40.1 ± 16.8 Mgha-1); perennial crops generally accumulate SOC through time,especially woody crops; and temperature was the main driver explainingdifferences in SOC dynamics, followed by crop age, soil bulk density, claycontent and depth. We present empirical evidence showing that the FAOperennialization strategy is reasonable, underscoring the role ofperennial crops as a useful component of climate change mitigationstrategies.

Impacts of integrated crop–livestock (ICL) systems on water quality have not been well studied. Water quality parameters were quantified for two 30‐min rainfall simulations in wheat (Triticum aestivum L.)–cover crop, cover crop, and rangeland grass control phases of a long‐term ICL study site in Mandan, ND, in 2017 and 2018. Both pre‐graze and post‐graze simulations were conducted. Nitrate‐N (NO3−–N), nitrite‐N (NO2−–N), ammonia‐N (NH4+–N), phosphate‐P (PO43−–P), and total suspended solid (TSS) concentrations and loads were evaluated in surface runoff water, along with concentrations of NO3−–N, NO2−–N, NH4+–N, and PO43−–P in infiltration water. Non‐parametric ranked two‐way analysis of variance was used to evaluate differences for vegetation type and grazing as fixed effects, with year as a random effect. Surface runoff concentrations of NO3−–N and NH4+–N were significantly different with cover crop and wheat phases being greater than grass. A grazing effect was also observed with pre‐graze significantly greater than post graze for NO2−–N, NH4+–N, PO43−–P, and TSS surface runoff concentrations. For infiltration water concentrations, similar significant effects of vegetation type were observed for NO3−–N and PO43−–P, while pre‐graze was also greater than post‐graze for NO2−–N and PO43−–P. Due to variable runoff volumes, no significant differences were observed for loads of any parameters. Minimal runoff volumes (0–6.7 L) and significant vegetation and grazing effects in nutrient infiltration water concentrations highlight the importance of infiltration in such studies, particularly at sites in the northern Great Plains.

Integrated crop−livestock (ICL) system effects on soil organic carbon (SOC) and total nitrogen (TN) are highly variable due to differences in ecoregion‐specific management practices, historical land use, and inherent climatic/edaphic attributes. Few studies have evaluated ICL system effects on SOC and TN in semiarid regions, where soil attributes change slowly. This study quantified ICL system effects on SOC and TN in a long‐term experiment near Mandan, ND. Effects of three residue management treatments (Grazed, Removed, Control) on SOC and TN were determined at five depths (0−0.08, 0.08−0.15, 0.15−0.31, 0.31−0.61, and 0.61−0.91 m) in 1999 and 2014. No‐till practices were employed throughout the study following conversion of perennial grass pastures. A significance criterion of P ≤ .10 was used to assess treatment effects as the experimental design was constrained to two replications. Residue management affected SOC and TN in near‐surface depths only, with greater concentration of SOC and TN at 0−0.08 m under Grazed compared to Removed in 2014 (P = .0844 and .0576). When expressed on an equivalent mass basis, SOC stocks were greater under Grazed and Control compared to Removed. Soil organic C and TN stocks increased over time in the Control by 11.2 Mg C ha−1 and 1.2 Mg N ha−1, respectively (P = .0728 and .0868). Soil organic C stocks also increased over time under the Grazed treatment by 10 Mg C ha−1 (P = .0630). Study results highlighted the importance of residue retention and livestock grazing for increasing SOC and TN in ICL systems under semiarid conditions.

Infield bale aggregation is essential for bale removal and for preparing the field for subsequent crops, which can be efficiently performed using the modern automatic bale picker (ABP) that supports multiple bales/trip (BPT) than commonly used tractors. But the energy involved in the bale aggregation logistics using ABP has not been thoroughly evaluated. Therefore, the energy involved in the bale aggregation, in terms of fuel consumption, was studied for different logistic scenarios using a tractor (control) and ABP with different variables such as field areas (8 to 259 ha), biomass yields (3-40 Mg ha-1), four outlet locations, and five equipment speeds (6.6-10.5 km h-1) using realistic equipment turning paths through a user-developed simulation program in `R.' Nebraska Tractor Test general method and fuel efficiency method were considered for the fuel consumption calculations. Fuel consumption for the ABP (8-259 ha) with 8 BPT on an average decreased by 72% and 53% compared to tractor with 1 and 2 BPT, respectively, based on logistics distance and equipment operation. Field area, biomass yield, and BPT were the most influential variables affecting logistics distance; while, field area, biomass yield, BPT, and equipment speed affecting the operation time and fuel quantity. Convenient prediction models (multi-variate nonlinear) for logistics distance, operation time, and fuel quantity, using the influential field variables, produced good fits (R2>=0.98). Overall, an ABP with a capacity of 8 BPT, which can also handle 11 BPT, is recommended considering the logistics energy.

Agriculture in the United States must respond to escalating demands for productivity and efficiency, as well as pressures to improve its stewardship of natural resources. Growing global population and changing diets, combined with a greater societal awareness of agriculture's role in delivering ecosystem services beyond food, feed, fiber, and energy production, require a comprehensive perspective on where and how US agriculture can be sustainably intensified, that is, made more productive without exacerbating local and off-site environmental concerns. The USDA's Long-Term Agroecosystem Research (LTAR) network is composed of 18 locations distributed across the contiguous United States working together to integrate national and local agricultural priorities and advance the sustainable intensification of US agriculture. We explore here the concept of sustainable intensification as a framework for defining strategies to enhance production, environmental, and rural prosperity outcomes from agricultural systems. We also elucidate the diversity of factors that have shaped the past and present conditions of cropland, rangeland, and pastureland agroecosystems represented by the LTAR network and identify priorities for research in the areas of production, resource conservation and environmental quality, and rural prosperity. Ultimately, integrated long-term research on sustainable intensification at the national scale is critical to developing practices and programs that can anticipate and address challenges before they become crises.

Sustainable ruminant livestock production could benefit from feedstuffs containing condensed tannin (CT) if they maintained or improved livestock health and productivity while reducing the excretion of urinary nitrogen (N), which can be readily lost to the environment. Such feedstuffs would be most useful if they were easy and inexpensive to grow and could be field dried and pelletized for ease of transport and storage. One feedstuff that may meet these criteria is pelleted sericea lespedeza (SL; Lespedeza cuneata). Therefore, a 4 × 4 Latin square trial was conducted with four mature male sheep fed four proportions of pelleted SL with alfalfa (Medicago sativa) hay cubes, to determine effects on digestion and N excretion, and the fate of the CT during digestion. Pelleted SL contained (g/kg dry matter; DM) 182 CT, 23.3N, 341 aNDF, 227 ADF, 87 ADL and 55 ash, while the cubed alfalfa hay contained (g/kg DM) 28.5N, 381 aNDF, 284 ADF, 78 ADL and 137 ash. The SL pellets were consumed readily, but increasing their proportion in the ration DM from 0 to 0.4 linearly decreased apparent digestibility of DM from 59.7 to 49.9% and N from 74.7 to 53.4%, with associated reductions in urinary N excretion. Evaluation with 1H-13C heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) spectroscopy detected trace amounts of intact CT in sheep feces and analysis of the feces from the 0.4 lespedeza pellet ration using the HCl-butanol-acetone-iron assay indicated that 96% of the CT had been modified into non-proanthocyanidin components. Fiber analysis conducted with and without sulfite indicated an accumulation of sulfite-resistant CT-protein complexes in feces which likely contributed to the reduction in digestibility of fiber and N. This study has shown that SL pellets reduced urinary N excretion and diverted feed N to feces, but their inclusion in the diet reduced the apparent digestibility of DM and N. Complexes of CT, protein and fiber were the main contributor to the poor digestibility of aNDF and ADF in diets containing SL pellets. Findings presented here suggest a potential negative effect of these SL pellets on long-term sheep productivity in contrast to short-term productivity improvements due to the ability of the SL pellets to reduce gastrointestinal parasite populations.

Increasing crop diversity has been proposed to increase the sustainability of cropping systems. If producers are to adopt these systems, they should also be economically viable. In this study conducted near Mandan, North Dakota, four no-till cropping systems with varying levels of crop diversity were evaluated over a 12-yr period to quantify system effect on crop productivity, input use, production costs, and economic risks and returns. Cropping system treatments included a small grain–fallow rotation (SG–Fallow) and a continuous spring wheat (Triticum aestivumL.) rotation (Cont SW) as baseline low-diversity rotations, a small grain–winter wheat (T. aestivumL.)–sunflower (Helianthus annuusL.) rotation (SG–WW–Sun), a 5-yr rotation (Five Year) and a dynamic rotation (Dynamic). The SG–Fallow rotation was significantly less productive and less profitable on average than the other rotations, as measured by gross returns and net returns, respectively. However, SG–Fallow also used significantly less inputs than the other rotations. Production costs for the Cont SW and SG–WW–Sun rotations showed a significant increasing trend over time, while production costs for the Five Year rotation showed a significantly lower and slight decreasing trend over the period, with cost trends for the SG–Fallow and Dynamic rotations intermediate to these. Net returns tended to increase and relative economic risk tended to decrease as crop diversity increased from SG–Fallow and Cont SW to SG–WW–Sun, Five Year and the Dynamic system. Results from this study suggest that more diverse rotations can maintain or increase crop productivity and enhance economic viability.

Understanding how agricultural management and climate change affect soil organic carbon (SOC) stocks is particularly important for dryland agriculture regions that have been losing SOC over time due to fallow and tillage practices, and it can lead to development of agricultural practice(s) that reduce the impact of climate change on crop production. The objectives of this study were: (i) to simulate SOC dynamics in the top 30 cm of soil during a 20-yr (1993-2012) field study using CQESTR, a process-based C model; (ii) to predict the impact of changes in management, crop production, and climate change from 2013 to 2032; and (iii) to identify the best dryland cropping systems to maintain or increase SOC stocks under projected climate change in central North Dakota. Intensifying crop rotations was predicted to have a greater impact on SOC stocks than tillage (minimum tillage [MT], no-till [NT]) during 2013 to 2032, as SOC was highly correlated to biomass input ( = 0.91, = 0.00053). Converting from a MT spring wheat (SW, L.)-fallow rotation to a NT continuous SW rotation increased annualized biomass additions by 2.77 Mg ha (82%) and SOC by 0.22 Mg C ha yr. Under the assumption that crop production will stay at the 1993 to 2012 average, climate change is predicted to have a minor impact on SOC (approximately -6.5%) relative to crop rotation management. The CQESTR model predicted that the addition of another SW or rye ( L.) crop would have a greater effect on SOC stocks (0- to 30-cm depth) than conversion from MT to NT or climate change from 2013 to 2032.

Graphical abstract, Measurement of greenhouse gas (GHG) flux using static chamber methods typically occurs immediately following sample collection. However, situations may arise requiring sample storage prior to analysis by gas chromatography. The objective of this study was to determine effects of storage time and temperature on carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) concentrations in vials containing “low” and “high” concentrations of certified standards. Samples were stored for 3, 7, 14, 28, and 84 days at four storage temperatures: room temperature, 25 °C, 4 °C, and −10 °C. Results indicated low and high concentration standards were not impacted by sample storage up to 28 days at any storage temperature. After 84 days, CO2 concentrations were 0.6–14.4% lower than expected while CH4 concentrations were up to 22% greater than expected. Results from future studies will allow for further refinement of scientifically supported guidance regarding appropriate storage temperature and time of GHG samples. • Few studies have examined impacts of storage time and temperature on GHG samples retained in traditional septa-capped vials. • Effects of storage time and temperature on GHG samples were examined. • Based on this study, GHG samples can be stored for up to 28 days at temperatures ranging from −10 °C to 25 °C.

Invasive nonnative grasses pose a significant threat to rangelands of the Northern Great Plains. Long-term data from a grazing experiment near Mandan, ND (46°46′11.43″N, 100°54′55.16″W) revealed the invasion of native prairie by Kentucky bluegrass, an exotic grass. We hypothesized that bluegrass invasion altered soil13C and15N levels, tracking the increased abundance of invasive cool-season grass aboveground. In 2014, soil samples were collected to depths of 0 to 7.6 cm and 7.6 to 15.2 cm in pastures grazed similarly since 1916. Samples were analyzed for total carbon (C) and nitrogen (N) and13C and15N isotopes and compared against archived samples from 1991. Vegetation change from native to exotic grasses changed the isotopic composition of soil C. The soil δ13C at the 0- to 7.6-cm depth became more negative between 1991 and 2014. Soil δ13C became less negative with increasing stocking rate at both soil depths. Soil δ15N values at the 0- to 7.6-cm depth decreased between 1991 and 2014. Soil δ15N increased with increasing stocking rate at the 0- to 7.6-cm depth in 2014. Soil C and N concentrations at 0 to 7.6 cm increased by 35% (12 g C kg−1) and 27% (0.9 g N kg−1), respectively, from 1991 to 2014; however, concentrations at the 7.6- to 15.2-cm depth did not change. The shift from native C4to invasive C3grass did not reduce soil C storage in the long-term prairie pastures. The more deleterious effect of invasion, however, may have been the buildup of dead biomass, which alters vegetation structure and may reduce native species’ diversity and abundance.

Perennial herbaceous biofeedstocks (PHB) have been proposed to confer multiple ecosystem services to agricultural lands. However, the role of PHBs to affect change in soil condition is not well documented, particularly for treatments with multiple species. The objective of this study was to quantify potential changes to soil properties resulting from PHB treatments in central and western North Dakota over a 5-yr period. Treatments with multiple perennial plant species were hypothesized to induce greater improvements in soil condition compared to monocultures. Soil properties were evaluated in seven PHB treatments (four monocultures, three mixtures) at five sites with sampling occurring immediately prior to treatment establishment in 2006 and again in 2011 across a 0 to 1.2 m depth. Perennial herbaceous biofeedstocks had minor and inconsistent effects on soil bulk density, electrical conductivity, and soil pH, and no effect on available P and soil organic C (SOC) in 2011. Contrasts between monoculture and mixtures in 2011 yielded no significant differences for any soil property at any site. However, PHB treatments did induce significant changes in soil properties between 2006 and 2011, with substantial declines in available P (> 10 kg P ha− 1 yr− 1) at sites with high initial P and modest increases in SOC (0.9–5.7 Mg C ha− 1 yr− 1) at sites with low initial SOC. Electrical conductivity decreased at two sites, though changes were minor (− 0.08 to − 0.18 dS m− 1). Soil pH did not change over the 5-yr study. Results from this study underscore the value of PHBs to remediate nutrient-laden and/or degraded soils, while concurrently resisting increased salinity and fertilizer-induced acidification.

Integrated crop-livestock systems can have subtle effects on soil quality over time, particularly in semiarid regions where soil responses to management occur slowly. We tested if analyzing temporal trajectories of soils could detect trends in soil quality data which were not detected using traditional statistical and index approaches. Principal component and cluster analyses were used to assess the evolution in ten soil properties at three sampling times within two production systems (annually cropped, perennial grass). Principal component 1 explained 33% of the total variance of the complete dataset and corresponded to gradients in extractable N, available P, and C : N ratio. Principal component 2 explained 25.4% of the variability and corresponded to gradients of soil pH, soil organic C, and total N. While previous analyses found no differences in Soil Quality Index (SQI) scores between production systems, annually cropped treatments and perennial grasslands were clearly distinguished by cluster analysis. Cluster analysis also identified greater dispersion between plots over time, suggesting an evolution in soil condition in response to management. Accordingly, multivariate statistical techniques serve as a valuable tool for analyzing data where responses to management are subtle or anticipated to occur slowly.

A major limitation to building credible soil carbon sequestration programs is the cost of measuring soil carbon change. Diffuse reflectance spectroscopy (DRS) is considered a viable low-cost alternative to traditional laboratory analysis of soil organic carbon (SOC). While numerous studies have shown that DRS can produce accurate and precise estimates of SOC across landscapes, whether DRS can detect subtle management induced changes in SOC at a given site has not been resolved. Here, we leverage archived soil samples from seven long-term research trials in the U.S. to test this question using mid infrared (MIR) spectroscopy coupled with the USDA-NRCS Kellogg Soil Survey Laboratory MIR spectral library. Overall, MIR-based estimates of SOC%, with samples scanned on a secondary instrument, were excellent with the root mean square error ranging from 0.10 to 0.33% across the seven sites. In all but two instances, the same statistically significant (p < 0.10) management effect was found using both the lab-based SOC% and MIR estimated SOC% data. Despite some additional uncertainty, primarily in the form of bias, these results suggest that large existing MIR spectral libraries can be operationalized in other laboratories for successful carbon monitoring.

Infield bale aggregation is essential for bale removal and preparing the field for subsequent crops, which can be more efficiently performed using the modern automatic bale picker (ABP) that supports multiple bales/trip (BPT) than commonly used tractors. But the energy involved in the bale aggregation logistics using ABP has not been thoroughly evaluated. Therefore, the energy involved in the bale aggregation, in terms of fuel consumption, was studied for different logistic scenarios using a tractor (control) and ABP through a user-developed simulation program in R. Different variables such as field areas (8 to 259 ha), biomass yields (3–40 Mg ha − 1 ), four outlet locations, and five equipment speeds (6.6–10.5 km h − 1 ) using realistic equipment turning paths were used in the simulation. The Nebraska Tractor Test general method and fuel efficiency method were considered for the fuel consumption calculations. Fuel consumption for the ABP (8–259 ha) with 8 BPT on an average decreased by 72 % and 53 % compared to a tractor with 1 and 2 BPT, respectively, based on logistics distance and equipment operation. Field area, biomass yield, and BPT were the most influential variables affecting logistics distance; while, field area, biomass yield, BPT, and equipment speed affecting the operation time and fuel quantity. Convenient prediction models (multi-variate nonlinear) for logistics distance, operation time, and fuel quantity, using the influential field variables, produced very good fits ( R 2 ≥ 0.98 ). Overall, an ABP with a capacity of 8 BPT, split which can also handle 11 BPT, is recommended considering the logistics energy.

Long-term use of nitrogen (N) fertilizers can lead to fertility-lowering soil chemical changes. To examine this in geologically young soils in the northern Great Plains of North America, we present near-surface (0–7.6 cm) soil chemistry data from 16 years of two crop rotations: continuous crop (CC; spring wheat [Triticum aestivum L.]—winter wheat [T. aestivum]—sunflower [Helianthus annuus L.]) and crop-fallow (C-F; spring wheat—fallow) that underwent factorial tillage (none, minimum, conventional) and N rate (low, medium, high) treatments. For CC, the N rate (but not tillage) had a significant effect on pH, with the high N rate leading to the largest pH decline (−0.76). The nitrogen rate also had a significant effect on cation exchange capacity (CEC) for CC, whereby CEC increased with the N rate. Managers utilizing high N rates should be aware of the potential for soil acidification, even in the northern Great Plains of North America.