1. Constitutive IFNα protein production in bats
- Author
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S. Le Poder, R. Wedlarski, Darragh Duffy, Vincent Bondet, D. Le Roux, Thierry Petit, Alexis Lécu, M. Le Baut, Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur [Paris], BioPôle Alfort, École nationale vétérinaire d'Alfort (ENVA), Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Muséum national d'Histoire naturelle (MNHN), Parc Zoologique de La Palmyre, Bioparc de Doué-la-Fontaine, Partenaires INRAE, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was funded by the Agence Nationale pour la Recherche (ANR), grant number CE17001002, We thank the ProteoGenix company (Schiltigheim, France) for the production of the bIFNα ELISA assay calibrator, Marielle Cochet for technical help, Florence Va, Dr Vitomir Djokic and Isabelle Badreau for helping in RT-qPCR datas analysis, the technical staff of the zoos and the Association Française des Vétérinaires de Parcs Zoologiques. We thank the CBUTechS platform of the Institut Pasteur for access to the Simoa instrument., Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), École nationale vétérinaire - Alfort (ENVA), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur [Paris]-Université Paris Cité (UPC), and École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
- Subjects
Transcription, Genetic ,[SDV]Life Sciences [q-bio] ,Immunology ,bats ,Enzyme-Linked Immunosorbent Assay ,Disease ,Biology ,Cell Line ,03 medical and health sciences ,Antiviral immunity ,Extant taxon ,Species Specificity ,Interferon ,Chiroptera ,Protein biosynthesis ,medicine ,Immunology and Allergy ,Animals ,Simoa digital ELISA ,Symbiosis ,030304 developmental biology ,Original Research ,Genetics ,0303 health sciences ,Chiroptera species ,Innate immune system ,Ifn response ,030306 microbiology ,Interferon-alpha ,Immunity, Innate ,antiviral immunity ,Gene Expression Regulation ,protein levels ,Host-Pathogen Interactions ,Viruses ,type I IFN ,medicine.drug - Abstract
Posté sur bioRxiv le 22 juin 2021.; International audience; Bats are the only mammals with self-powered flight and account for 20% of all extant mammalian diversity. In addition, they harbor many emerging and reemerging viruses, including multiple coronaviruses, several of which are highly pathogenic in other mammals, but cause no disease in bats. How this relationship between bats and viruses exists is not yet fully understood. Existing evidence supports a specific role for the innate immune system, in particular type I interferon (IFN) responses, a major component of antiviral immunity. Previous studies in bats have shown that components of the IFN pathway are constitutively activated at the transcriptional level. In this study, we tested the hypothesis that the type I IFN response in bats is also constitutively activated at the protein level. For this we utilized highly sensitive Single Molecule (Simoa) digital ELISA assays, previously developed for humans that we adapted to bat samples. We prospectively sampled four non-native chiroptera species from French zoos. We identified a constitutive expression of IFNα protein in the circulation of healthy bats, and concentrations that are physiologically active in humans. Expression levels differed according to the species examined, but was not associated with age, sex, or health status suggesting constitutive IFNα protein expression independent of disease. These results confirm a unique IFN response in bat species that may explain their ability to coexist with multiple viruses in the absence of pathology. These results may help to manage potential zoonotic viral reservoirs and potentially identify new anti-viral strategies.
- Published
- 2021