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Constitutive IFNα protein production in bats
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Frontiers, 2021, 12, pp.735866. ⟨10.3389/fimmu.2021.735866⟩, Frontiers in Immunology, 2021, 12, pp.735866. ⟨10.3389/fimmu.2021.735866⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- Posté sur bioRxiv le 22 juin 2021.; International audience; Bats are the only mammals with self-powered flight and account for 20% of all extant mammalian diversity. In addition, they harbor many emerging and reemerging viruses, including multiple coronaviruses, several of which are highly pathogenic in other mammals, but cause no disease in bats. How this relationship between bats and viruses exists is not yet fully understood. Existing evidence supports a specific role for the innate immune system, in particular type I interferon (IFN) responses, a major component of antiviral immunity. Previous studies in bats have shown that components of the IFN pathway are constitutively activated at the transcriptional level. In this study, we tested the hypothesis that the type I IFN response in bats is also constitutively activated at the protein level. For this we utilized highly sensitive Single Molecule (Simoa) digital ELISA assays, previously developed for humans that we adapted to bat samples. We prospectively sampled four non-native chiroptera species from French zoos. We identified a constitutive expression of IFNα protein in the circulation of healthy bats, and concentrations that are physiologically active in humans. Expression levels differed according to the species examined, but was not associated with age, sex, or health status suggesting constitutive IFNα protein expression independent of disease. These results confirm a unique IFN response in bat species that may explain their ability to coexist with multiple viruses in the absence of pathology. These results may help to manage potential zoonotic viral reservoirs and potentially identify new anti-viral strategies.
- Subjects :
- Transcription, Genetic
[SDV]Life Sciences [q-bio]
Immunology
bats
Enzyme-Linked Immunosorbent Assay
Disease
Biology
Cell Line
03 medical and health sciences
Antiviral immunity
Extant taxon
Species Specificity
Interferon
Chiroptera
Protein biosynthesis
medicine
Immunology and Allergy
Animals
Simoa digital ELISA
Symbiosis
030304 developmental biology
Original Research
Genetics
0303 health sciences
Chiroptera species
Innate immune system
Ifn response
030306 microbiology
Interferon-alpha
Immunity, Innate
antiviral immunity
Gene Expression Regulation
protein levels
Host-Pathogen Interactions
Viruses
type I IFN
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology, Frontiers in Immunology, Frontiers, 2021, 12, pp.735866. ⟨10.3389/fimmu.2021.735866⟩, Frontiers in Immunology, 2021, 12, pp.735866. ⟨10.3389/fimmu.2021.735866⟩
- Accession number :
- edsair.doi.dedup.....bfb0bbedcc35932b251dcd981e488bc4