Search

This review aimed to analyze the scientific literature on pancreatic diseases (especially exocrine pancreatic insufficiency). This review also describes the correlation between the physiological fitness of the pancreas and obesity. The influence of the pancreatic exocrine function on the development of the organism of adults and adolescents was also described. The results of piglet studies available in the literature were cited as an established model used to optimize treatments for pancreatic diseases in humans. The pancreas has an exocrine and hormonal function. Consequently, it is one of the key internal organs in animals and humans. Pancreatic diseases are usually severe and particularly troublesome. A properly composed diet and taken dietary supplements significantly improve the patient’s well-being, as well as the course of the disease. Therefore, a diet and a healthy lifestyle positively affect maintaining the optimal physiological efficiency of the pancreas.

Ultra-fast liquid chromatography (UFLC) with a photodiode array detector (DAD) for simple and rapid determination of orotic acid (OAc) in milk of sheep and cows is described. Milk samples are treated with acetonitrile (1:1, v/v) and then centrifuged at 4 °C. To 1 mL of the obtained supernatant 9 mL of ultrapure water was added. Subsequently, 0.5–6 µL of the resulting solution was injected into the UFLC-DAD system. Separation and quantification of OAc in milk samples was achieved using two Kinetex C18 columns (1.7 µm, 150 mm × 2.1 mm, i.d., 100 Å, Phenomenex) fitted with a pre-column of 4 mm × 2 mm, i.d. (Phenomenex) containing C18 packing material. All separations were performed at a column temperature of 35 °C while the ambient temperature was 21–24 °C. Satisfactory separation of OAc from endogenous species of milk can be achieved using the binary gradient elution program and UV detection at wavelengths 278 nm. Our original procedure resulted in suitable separation and quantification of OAc in milk samples, OAc eluted at 6.44 ± 0.03 min. The total run time of OAc analysis (including re-equilibration) was 27 min. As expected, the OAc peak was absent from the blank when the proposed gradient elution program and UV detection at 278 nm was used. The average recoveries of OAc standards added to milk samples were satisfactory (96.7–105.3%). The low inter-and intra-assay coefficient of variation derived from the measurements of OAc in cow and ovine milk samples (i.e., 0.784%, 1.283% and 0.710%, 1.221%, respectively) and in O-Ac standards (i.e., 0.377% and 0.294%, respectively), as well as high recoveries of OAc added to ovine and cows’ milk (~100%) and the low detection (0.04 ng) and quantification (0.12 ng) limits point to satisfactory accuracy, precision and sensitivity of the reported method. OAc concentrations in ovine milk samples were within the range from 25 to 36 mg/L, while OAc levels in cows’ milk samples was found in the range of 32–36 mg/L. Our original procedure is suitable for routine quantification of OAc in milk of ewes and cows.

The main aim of our study was to investigate the impact of carnosic acid (CA), selenized yeast (SeY) and selenate (SeVI) added to the diet including rapeseed oil (RO) and fish oil (FO) on contents of volatile fatty acids (VFA), fatty acids (FA), CH4 and CO2 in the rumen and levels of FA, tocopherols, total cholesterol (TCl) and malondialdehyde (MDA; a marker of oxidative stress) in the rumen-surrounding fat (RSF) of lambs. The second objective of our study was to investigate the effects of CA and Se (as SeY or SeVI) added to the diets including RO and FO on concentrations of FA in the liver, heart, musculus longissimus dorsi (MLD) and musculus biceps femoris (MBF) of lambs. After the preliminary period, the 35-day experiment was conducted, during which animals were fed the basal diet (BD) with 20 g RO/kg BD and 10 g FO/kg BD (the control diet) or experimental diets supplemented with 20 g RO/kg BD, 10 g FO/kg BD and 1 g CA/kg BD without/with 0.35 mg Se/kg BD (Se as SeY or SeVI). The experimental diet with CA and SeY increased ruminal contents of acetic acid (AA), propionic acid (PA), butyric acid (BA), valeric acid (VA), iso-butyric acid (isoBA), iso-valeric acid (isoVA), CH4 and CO2 compared to the control and other experimental diets. The experimental diet with CA and SeVI decreased the ruminal contents of BA, VA, isoBA, the content sums of VFA and straight-chain VFA, CH4 and CO2 compared to the control diet and the experimental diet with CA and SeY. All experimental diets affected the concentration of FA in the RSF, ruminal fluids, the liver, heart, MLD and MBF. Content sums of long-chain polyunsaturated FA (LPUFA), n-3LPUFA and ratios of LPUFA/FA, n-3LPUFA/FA and Σn-3LPUFA/n-6PUFA in the RSF of lambs fed all of the experimental diets was lower than that of the control diet. SeY and CA added to the diet reduced the content of MDA in the RSF compared to the control and other experimental diets. The diet with CA and SeVI decreased the level of MDA in the RSF when compared to the control diet and the diet with CA. Compared to SeY added to the diet with CA, the diet containing CA and SeVI more efficiently improved animal performance by reducing lamb emissions of CH4 and CO2 (the greenhouse gases); this is valuable information for nutritionists carrying out research on farm ruminants to improve the nutritive value of food from the aspect of human health.

Rumen protozoa are known to contribute to fibre digestion, but the fibrolytic enzymes of the majority of ciliate species have been poorly recognised to date. The aims of the study were, first, to determine the influence of crystalline cellulose on the survival and population density of the ciliate Diploplastron affine when cultured in vitro, and second to identify and characterise the protozoal enzymes catalysing the hydrolysis of cellulose. It was found that crystalline cellulose, when added to a culture medium, increased the number of protozoa maintained in vitro. We observed that the bacteria-free ciliates fermented microcrystalline cellulose and produced 43.3 nmol volatile fatty acids/protozoon/h. A cell extract prepared from the bacteria-free ciliates degraded crystalline cellulose in the rate of 11.5 nmol released glucose/mg protein/min, whereas the degradation rates of carboxymethyl-cellulose (CMC), avicel and cellobiose were 343, 6.8 and 145 nmol released glucose/mg protein/min respectively. Two distinct peaks in the activity of relevant enzymes were identified following ion exchange chromatography of the protozoal cell extract and the presence of two different CMC-ases were confirmed by zymographic studies. CMC was mainly degraded to mono- and disaccharides but that some other oligosaccharides were also present. Cellobiose was the only product of avicel digestion.

Simple Summary The current study investigated the bone status of growing lambs fed diets supplemented with bioactive components (fish oil, carnosic acid, SeY, and Na2SeO3) improving bone parameters. The study provides new information with regards to the positive role of bioactive components supplemented to diets for growing lambs on their femur characteristics (bone content, bone mineral density, geometry, and strength). Abstract The aim of the present study was to assess the effects of partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants on the characteristics of lamb femur. Thirty male lambs were assigned to five dietary treatments and fed isoproteinous and isoenergetic diets for 35 days. The control diet was enriched with 3.0% RO, while the experimental diets were enriched either only with 2.0% RO and 1.0% FO or additionally with 0.1% carnosic acid, 0.1% carnosic acid and 0.35 ppm Se as selenized yeast, or 0.1% carnosic acid and 0.35 ppm Se as sodium selenite. After 35 days, the lambs were slaughtered, and the femur was dissected from the carcass of each animal and analyzed for morphometric, geometric, densitometric, and biomechanical properties. The dietary modifications, specifically the supplementation of FO and selenized yeast, significantly improved the geometric, densitometric, and biomechanical properties of lamb femur.

The aim of our study was to investigate the impact of carnosic acid (CA), selenate (VISe) or selenized yeast (YSe) on concentrations of fatty acids (FA), tocopherols, cholesterol and malondialdehyde in the periintestinal fat (PIF) and muscles of lambs. Male lambs were fed the control diet containing rapeseed (RO) and fish (FO) oils, the CA diet containing RO, FO and CA, the YSe-CA diet with RO, FO, CA and YSe, and the VISe-CA diet with RO, FO, CA and VISe. The experimental diets with CA, irrespective of the presence of YSe or VISe, decreased sums of saturated FA (SFA) and the thrombogenic SFA in the PIF compared to the control. The experimental diets increased the Δ9-desaturation capacity in the PIF compared to the control. The experimental diets with YSe or VISe reduced sums of long-chain polyunsaturated FA in the PIF compared to the control and CA diets. The PIF and muscles of lambs fed the VISe-CA diet were characterised by the highest hypocholesterolemic/hypercholesterolemic-FA ratio, and lower modified atherogenic index compared to the control.

Background: Lambs or sheep are considered an excellent animal model for humans owing to advantages related to bone anatomy, formation, biomechanical characteristics; bone strength; and absorption of minerals and vitamins. Moreover, bone healing in many animal species is faster than that in humans, whereas bone healing, turnover, and remodelling in sheep and humans are comparable. In this context, it would be interesting to examine the effects of bioactive components of diet (including Se and carnosic acid) on bone mineralisation and strength, since these are the most important indicators of bone status. This knowledge may be useful in the context of orthopaedic research, recovery after orthopaedic surgeries, and prevention of skeletal diseases in humans.Results: The aim of the present study was to assess the effects partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants on the characteristics of lamb femur. Thirty male lambs were assigned to five dietary treatments and fed isoproteinous and isoenergetic diets for 35 days. The control diet was enriched with 3.0% RO, while the experimental diets were enriched either only with 2.0% RO and 1.0% FO or additionally with 0.1% carnosic acid, 0.1% carnosic acid and 0.35 ppm Se as selenised yeast, or 0.1% carnosic acid and 0.35 ppm Se as sodium selenite. After 35 days, the lambs were slaughtered, and the femur was dissected from the carcass of each animal and analysed for morphometric properties.Conclusions: The present study indicated that dietary bioactive components may improve bone health by promoting bone mineralisation in lambs. Partial replacement of RO with FO combined with dietary supplementation of carnosic acid and organic Se improved the geometric, densitometric, and biomechanical properties of lamb femur.

A close link between cardiovascular diseases and cancer results from sharing the same modifiable risk factors (e.g. nutritional) and cardiotoxicity of anti-cancerous therapies. It justifies cardio-oncological preliminary studies on dietary factors, especially on those of possible anti-carcinogenic or cardioprotective properties. The main purpose was to evaluate the effect of pomegranate seed oil (PSO) and/or bitter melon extract (BME) supplementation of the diet of female rats suffering from mammary tumors on lipidomic profile (expressed as fatty acids, conjugated fatty acids (CFA), malondialdehyde (MDA), cholesterol and oxysterols content) of cardiac tissue. Total lipidomic profile and intensity of lipid peroxidation in hearts of DMBA-treated Sprague-Dawley rats and their healthy equivalents, both obtaining diet supplementation, were evaluated with different chromatographic techniques coupled with appropriate detection systems (GC-MS, GC-TOFMS, Ag+-HPLC-DAD, UF-HPLC-DAD). Dietary modifications neither diminished breast cancer incidence nor exerted explicit cardio-protective influence, however, they diminished cholesterol content, i.a. because of inhibition of the endogenous conversion of squalene to cholesterol in cardiac tissue. CFA were incorporated into cardiac tissue to a lesser extent in the cancerous process. PSO and BME anti-oxidant properties in pathological condition were only slightly reflected in MDA levels but not in oxysterols formation. Obtained results indicate considerable changes in dietary supplements' biological activity in pathological conditions and the need for clear distinction of drugs and dietary supplements, which is of utmost importance, especially for cancer survivors.

Obesity and high cholesterolaemia are major health problems in industrialized countries. The effects of the antiobesity drug orlistat at 0.3 g kg-1and amidated alginate at 40 g kg-1on serum and hepatic cholesterol and the faecal output of fat and sterols were compared in female rats. Rats were fed diets containing cholesterol and palm fat at 10 and 70 g kg-1, respectively. Palm fat was provided by coconut meal. Amidated alginate (the octadecylamide of alginic acid) is a sorbent of lipids, and orlistat (tetrahydrolipstatin) is an inhibitor of pancreatic lipase. Both agents significantly increased the faecal loss of fat, orlistat, however, did not significantly decrease serum total cholesterol and its effect on hepatic cholesterol was less pronounced. Amidated alginate at 40 g kg-1significantly decreased serum total cholesterol, LDL cholesterol, hepatic cholesterol, and hepatic lipids, and increased the faecal output of fat and coprostanol (a metabolite of cholesterol). Both orlistat and amidated alginate modified the fatty acid profile in excreted lipids. The concentration of saturated fatty acids decreased and the concentration of unsaturated fatty acids increased. Despite different modes of action, orlistat and amidated alginate were equally efficient in the removing dietary fat from the body. Amidated alginate, however, was more active in the control of serum and hepatic lipid metabolism.

Background The ‘developmental origin of health and disease’ (DOHaD) hypothesis assumes that due to the action of some stimuli during fetal life the long-term physiological changes occurs and may affects the risk of various diseases. The main aim of this study was to assess impact of supplementation of maternal’ and early postnatal diet with conjugated linoleic acids (CLA) isomers on selected elements content in hearts of their female offspring with chemically induced breast cancer. Methods Elemental composition was determined by quadrupole mass spectrometer with inductively coupled plasma ionization (ICP-MS). Results The effect of maternal’ diet on the elements content was more pronounced than the progeny diet modifications. Significant correlations among determined elements, especially macroelements, and lipidomic parameters, in the experimental factors dependent manner were observed. It can be concluded that supplementation of maternal and progeny diets with CLA isomers may significantly influence the contents of both macro- and microelements in cardiac tissue of newborns. Conclusion Our results also indicate, that dynamic and intricate balance among various elements in body may be affected by the lipid dietary supplements also in the pathological state. Utility of cardio-oncological approach in developmental programming study was confirmed.

The aim of the current study was to evaluate the impact of carnosic acid (CA), selenised yeast (YSe) and selenate (VISe) supplemented to diets, including fish oil (FO) and rapeseed oil (RO), on the content of fatty acids, total cholesterol (TCh), tocopherols and malondialdehyde in the fat located between the thigh muscles and the heart in lambs. Twenty-four male Corriedale lambs were divided into 4 groups of 6 animals. Animals were fed a diet with FO and RO (the control diet) or experimental diets containing RO, FO and CA with/without Se (as YSe or VISe). The experimental diets without/with YSe or VISe changed concentrations of fatty acids in the fat and heart compared to the control. All experimental diets increased the levels of c11c14C20:2, c5c8c11c14C20:4, c5c8c11c14c17C20:5 and the sums of long-chain polyunsaturated fatty acids (LPUFA) and conjugated linoleic acid isomers in the fat compared to the control. The experimental diet containing YSe or VISe increased the content of Se, TCh, c11c14C20:2, c8c11c14C20:3, c5c8c11c14C20:4, c5c8c11c14c17C20:5, c7c10c13c16c19C22:5, c4c7c10c13c16c19C22:6 and the concentration sum of n-3LPUFA, n-6LPUFA and tocopherols in the heart in comparison with the control diet and the diet containing only CA. Experimental diets reduced the concentration of malondialdehyde in the fat and heart in comparison with the control diet. Our dietary intervention has great potential for future practical and commercial implementations.

Identification and quantification of the selenium species in biological tissues is imperative, considering the need to properly understand its metabolism and its importance in various field of sciences, especially nutrition science. Although a number of studies deals with the speciation of selenium, speciation analysis is still far from being a routine task, and so far strongly depends on the type of the samples. We present a study aimed to examine speciation analysis of Se in tissues of livers, muscles, and hearts obtained from lambs, namely in liver, muscle, and heart. The studied lambs were fed with the diet enriched with an inorganic (as sodium selenate) and organic chemical form of Se (as Se-enriched yeast) compounds with simultaneous addition of fish oil (FO) and carnosic acid (CA). The first part of the work was focused on the optimization of the extraction procedure of selenium compounds from tissues. Next, hyphenated high performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC&ndash, ICP&ndash, MS) was used for the identification of five seleno-compounds&mdash, Se-methionine (SeMet), Se-cystine (SeCys2), Se-methyl-Se-cysteine (SeMetSeCys), and Se(IV) and Se(VI). Verification of the identified seleno-compounds was achieved using triple-quadrupole mass spectrometer coupled to high performance liquid chromatography (HPLC&ndash, ESI&ndash, MS/MS). The applied procedure allowed for quantitative analysis of SeMet, SeCys2, and SeMetSeCys, in biological tissues. The developed analytical protocol is feasible for speciation analysis of small molecular seleno-compounds in animals samples.

Linking the early life environment with later health status is known as &ldquo, developmental programming&rdquo, This study aimed to assess whether the introduction of conjugated linoleic acids (CLAs) into the maternal diet affects the content fatty acids (FAs), conjugated FAs (CFAs), cholesterol, oxysterols, malondialdehyde (MDA) and tocopherols in the hearts of their female offspring treated with 7,12-dimethylbenz[a]anthracene and if offspring supplementation enhanced the effect of maternal supplementation. FA, cholesterol and oxysterol contents were determined by gas chromatography-mass spectrometry, while contents of CFAs and MDA were determined by high-performance liquid chromatography (HPLC) with photodiode detection. The supplementation of mothers with CLAs significantly decreased the amount of atherogenic saturated FAs and enhanced the level of eicosapentaenoic FA in the hearts of offspring. Continuous progeny supplementation decreased the content of arachidonic acid in hearts. Supplementation of the maternal diet with CLAs and its continuation during the postnatal period increased the ratio of hypo to hypercholesterolemic FAs. Significantly fewer oxysterols were detected in the hearts of progeny of dams fed with CLAs as compared to the offspring of mothers receiving safflower oil. Both fetal and postnatal CLA intake significantly reduced 7&beta, hydroxycholesterol accumulation. It can be concluded that CLA supplementation during the fetal and postnatal period may be an effective method of maintaining the cardiac health status of newborns.

IntroductionPomegranate (Punica granatum, Punicaceae) seed oil (PSO) and bitter melon (Momordica charantia, Cucurbitaceae) water extract (BME), used for centuries in folk medicine, are now available as popular dietary supplements, used i.e. in prevention of diet-related metabolic diseases and cancer. It is suggested that their mechanism of action may involve modifications of oxidative processes. However, information regarding these effects is inadequate.AimThis study aimed to evaluate the influence of supplementation of rats’ diet with PSO and BME, administered separately or jointly, on cholesterol and its oxidative derivatives, malonyldialdehyde (MDA) and tocopherols content in livers, in terms of cancerous process. Sprague-Dawley female rats (n = 96) were fed control diet (CON and CONplus) or experimental diets supplemented with 0.15 ml PSO/day (G and Gplus), 1% BME ad libitum (M and Mplus) and PSO and BME jointly (GM and GMplus). Breast cancer was induced by administration of 7,12-dimethylbenz[a]anthracene (DMBA) in the amount of 80 mg/kg body weight in the 50th day of life. After 21 weeks of supplementation livers, as main organs responsible for lipids metabolism, were subjected to either GC-TOFMS (cholesterol and oxysterols) or UPLC-PDA (tocopherols and MDA) chromatographic analyzes.Results and discussionBoth dietary supplements and DMBA administration resulted in significant increase of total cholesterol content. In contrast, levels of two of four determined oxysterols: 7α - hydroxycholesterol and cholesterol β-epoxide were the lowest in groups supplemented with PSO and exposed to DMBA. Significant differences in tocopherol content were demonstrated for two of four detected compounds: g-tocopherol and α-tocopheryl acetate. The level of g-tocopherol in G group was significantly higher in relation to Mplus and Gplus groups. In case of α -tocopheryl acetate, differences were observed between two control groups (CON and CONplus), but its content in CONplus group was significantly higher. In physiological state applied supplements reduced MDA levels in livers, whereas in pathological conditions PSO significantly increased MDA content in hepatic tissue of Gplus and GMplus groups.ConclusionsThe obtained results indicate that applied dietary supplementation, as well as cancerous process, can modify the levels of cholesterol and intensity of oxidative processes. It seems, that oxidative stress, associated with cancer development, modifies the intensity of oxidative processes in livers to a greater extent than examined dietary supplements.* This work was partially supported by National Science Centre, Poland (grant number 2018/02/X/NZ9/01770)

The main goal of the study was to determine the influence of the addition for 6 weeks of 0.25 ppm Se as selenate (SeVI) or selenised yeast (SeY) to an isoenergetic and isoproteinous basal diet containing 2% fish oil, 2% sunflower oil and 12 ppm lycopene on the concentrations of fatty acids (FA), especially mono- (MUFA) and poly-unsaturated (PUFA) fatty acids in the liver, spleen and patch of broiler chickens. The effects of these supplements on the yield of PUFA peroxidation in said internal organs of the chickens were also investigated. SeVI or SeY added to the diet decreased the total concentration of saturated FA (SFA), especially atherogenic- (A-SFA) and thrombogenic (T-SFA) SFA in the liver compared to the control group. The diet with SeVI reduced the concentration sum of MUFA, the yield of PUFA peroxidation and the capacity of Δ9-desaturation of C16:0 and C18:0 in the liver of chickens compared to the control and SeY diets. The yield of PUFA peroxidation was higher in the liver and patch of chickens fed the diet with SeY than in the control and SeVI diet. SeVI or SeY added to the diet decreased the PUFAn-6 to PUFAn-3 ratio in the liver, spleen and patch of chickens. The diets with SeVI and SeY usually revealed a negligible influence on the content sum of A-SFA, T-SFA, SFA, MUFA and PUFA in the spleen and patch of chickens compared to the control diet. Further studies are necessary to determine if dietary fish and sunflower oils, as well as tocopherols and other Se-compounds (like selenite or seleno-cysteine), affect the concentrations of FA in the edible parts of carcasses of chickens that are beneficial for humans.

The purpose of this study was to assess if partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants (carnosic acid – CA, selenized yeast – SeY and selenate – Se(VI)) influences the profile of biohydrogenation products, intermediates and conjugated fatty acids (CFA) in the rumen content and selected tissues of lambs. Thirty Corriedale male lambs were divided into 5 groups and fed for 35 days isonitrogenous and isoenergetic diets: basal diet enriched with 30 g RO/kg (Group 0 – control), diet enriched with 20 g RO/kg and 10 g FO/kg (Group I) or diets with combined addition of 20 g RO/kg, 10 g FO/kg and antioxidants (Group II supplemented with 1 g CA/kg; Group III with 1 g CA/kg and 0.35 mg/kg Se as SeY; Group IV with 0.35 mg/kg Se as Se(VI)). Applied dietary modification significantly affected the lipid transformation in the rumen. Replacement of RO by FO to the greater extent affected the profile of conjugated than non-conjugated intermediates in all samples. Collateral supplementation of antioxidants acted synergistically with FO in case of CFA content in the rumen content whereas in tissues the impact of antioxidants was antagonistic to FO. Se-compounds influenced CFA amounts in the most potent way, although dependent on their chemical form. Inorganic form (Se(VI)) reduced conjugated biohydrogenation intermediates contents in lamb livers while its organic chemical form (SeY) stimulated deposition of CFA and CD in femoral muscles. Partial replacement of RO by FO resulted in preferential incorporation of CFA isomers into liver and dorsal muscle. A complex insight into PUFA metabolism in the rumen was possible due to consideration of both steps (isomerisation and biohydrogenation) as well as plethora of possible intermediates and arising products (especially conjugated). Our results indicate that isomerisation and biohydrogenation are strongly affected by the Se-compounds. The influenced depended on the chemical form of these compounds: SeY strongly influenced the isomerization of both LA and ALA, whereas inorganic Se modified the biohydrogenation of ALA. So, it can be stated that aside from phytochemicals, Se-compounds have also an ability to modify processes occurring in rumen and thus may lead to favorable modification of FA profile of edible part of ruminant carcass, which is of utmost importance from the consumer point of view.

The objective of our studies was to investigate effects of carnosic acid (CA), selenized yeast (SeY) and selenate (SeVI) added to the diet including rapeseed oil (RO) and fish oil (FO) on concentrations of elements, fatty acids (FAs), tocopherols, cholesterol, and malondialdehyde in the liver, heart, musculus longissimus dorsi (MLD), and musculus biceps femoris (MBF) of lambs. Lambs were fed diets: group I-the basal diet (BD) with RO; group II-BD with RO and FO; group III-BD with RO, FO, and CA; group IV-BD with RO, FO, CA, and SeY; group V-BD with RO, FO, CA, and SeVI. The diets with Se compounds increased Se concentrations in all tissues compared with other diets. The diet with SeVI increased Cd, Sb, and Pb concentrations in the liver compared to groups I, II, and IV. The diets containing Se compounds increased Sb and Pb concentrations in MBF compared to groups I and II. All diets with CA reduced As, Sb, and Pb concentrations in MLD compared to groups I and II. All diets with FO increased concentrations of FAs and malondialdehyde in the liver compared to group I. All diets with FO decreased FAs concentrations in MBF compared to group I. The diets containing CA with/without Se compounds increased malondialdehyde concentrations in MBF compared to groups I and II. The diet with Se compounds reduced malondialdehyde concentrations in MLD compared to group II. All diets with FO changed concentrations of tocopherols and cholesterol in all tissues compared to group I. Our study showed that the addition of SeY or SeVI to the experimental diet increased the concentration of Se in all assayed tissues of lambs without adversely influencing performance or causing physiological disorders in internal organs. Both, SeY or SeVI added to the experimental diet decreased the oxidative stress and the concentrations of As, Sb, and Pb in MLD compared with the diets containing RO, irrespective of the presence of FO (groups I and II). Our study provides useful knowledge for nutritionists carrying out further investigations aimed at improving farm animal health, performance, and the nutritional quality of animal products for humans.

The aim of the study was to verify in a cardio-oncological model experiment if conjugated linoleic acids (CLA) fed to rats with mammary tumors affect the content of selected macro- and microelements in their myocardium. The diet of Sprague–Dawley females was supplemented either with CLA isomers or with safflower oil. In hearts of rats suffering from breast cancer, selected elements were analyzed with a quadrupole mass spectrometer with inductively coupled plasma ionization (ICP-MS). In order to better understand the data trends, cluster analysis, principal component analysis and linear discriminant analysis were applied. Mammary tumors influenced macro- and microelements content in the myocardium to a greater extent than applied diet supplementation. Significant influences of diet (p = 0.0192), mammary tumors (p = 0.0200) and interactions of both factors (p = 0.0151) were documented in terms of Fe content. CLA significantly decreased the contents of Cu and Mn (p = 0.0158 and p = 0.0265, respectively). The level of Ni was significantly higher (p = 0.0073), which was more pronounced in groups supplemented with CLA. The obtained results confirmed antioxidant properties of CLA and the relationship with Se deposition. Chemometric techniques distinctly showed that the coexisting pathological process induced differences to the greater extent than diet supplementation in the elemental content in the myocardium, which may impinge on cardiac tissue’s susceptibility to injuries.

The aim of our investigation was to study the impact of lycopene (Lyco) and selenized yeast (Se-Y) supplemented to the diet containing fish oil (F-O) on the profile of fatty acids (FA) in the liver and musculus biceps femoris (MBF) of rabbits. Rabbits were divided into 4 groups of 10 individuals and were fed ad libitum with the control and experimental diets for 55 days. The control diet was the basal diet (B-D) with F-O, whereas three experimental diets were the Lyco diet (the B-D with F-O and Lyco), the Se diet (the B-D with F-O and Se-Y) and the Se-Lyco diet (the B-D with F-O, Lyco and Se-Y). The Se-Lyco diet increased the content sums of all assayed FA (∑FA), saturated FA (SFA), atherogenic SFA and thrombogenic SFA in the liver and MBF compared to the control, Lyco and Se diets. Contents of cis9C16:1 (c9C16:1), c9C18:1 and c12C18:1, content sums of monounsaturated FA (∑MUFA) and odd-chain MUFA (∑Odd-MUFA), Δ9-desaturas indices and content ratios of ∑MUFA to ∑FA, and ∑Odd-MUFA to ∑FA in the liver of rabbits fed all experimental diets were lower than that of the control diet. Contents of trans10cis12C18:2, c9c12C18:2, c9c12c15C18:3, c11c14C20:2, c8c11c14C20:3, c5c8c11c14C20:4, c5c8c11c14c17C20:5, c7c10c13c16c19C22:5, c4c7c10c13c16c19C22:6 and content sums of n-6 polyunsaturated FA (n-6PUFA), n-3PUFA, PUFA, long-chain n-6PUFA (n-6LPUFA), n-3LPUFA and LPUFA in the liver of rabbits fed the diet containing Lyco without or with of Se-Y compared to the control diet. Feed supplementation with Lyco and Se-Y significantly stimulated the accumulation of n-3LPUFA in the MBF and liver and decreased ratios of the content sum of n-6PUFA to n-3PUFA and n-6LPUFA to n-3LPUFA in the MBF of rabbits. Lyco and Se-Y should be added to diets with F-O to improve the nutritive value of rabbits’ meat as it relates to human health.

The objective of this study was to evaluate the physicochemical characteristics of the longissimus muscle of lambs fed a control diet containing 3 % rapeseed oil (RO) (group I); an experimental diet with 2 % RO and 1 % fish oil (FO) (group II); or experimental diets with coupled addition of 2 % RO, 1 % FO, and 0.1 % carnosic acid (CA) (group III) without/with 0.35 ppm Se as selenized yeast (SeY) (group IV) or selenate (SeVI as sodium selenate, Na2SeO4) (group V). The results showed that dietary FO or SeVI affected the ultimate pH and meat color in comparison to lambs fed the control diet. The differences noted between diets in the case of muscle tissue were as follows: myosin HC (heavy chain) and LC1 (light chain), 150 kDa, α-actinin, 60 kDa, and TnT (troponin T), 30 kDa protein. The addition of FO to lamb diets resulted in a lower content of 30 kDa proteins, which are indicators of proteolysis. Considering the protein profile of drip loss, results showed effects of dietary administration of FO, CA, and Se (as SeY and SeVI) on the abundance of the following sarcoplasmic proteins: AMPDA (AMP deaminase), PGM (phosphoglucomutase), PK/PGI (pyruvate kinase/phosphoglucose isomerase), CK/PGAK (creatine kinase/phosphoglycerate kinase), ALD (aldolase), LDH (lactate dehydrogenase), PGAM (phosphoglycerate mutase), and TPI (triosephosphate isomerase). The lower content of TPI (in group II with comparison to groups III–V) and PGAM (in group II with comparison to group I and III) could be due to a higher ultimate pH in the group with FO addition, whereas lower contents of CK/PGAK, ALD, and LDH were attributed to Se addition. In comparison to the control diet, all experimental diets without SeVI decreased the content of the sum of all assayed amino acids ( ∑ AAs), indispensable amino acids ( ∑ IAAs), and dispensable amino acids ( ∑ DAAs) in the muscle. The highest ratios of ∑ IAAs to ∑ DAAs and ∑ IAAs to ∑ AAs were found in the muscle of lambs fed the diet containing RO, FO, and CA. The FO-supplemented diet with CA and SeVI increased the abundance of methionine in the muscle in comparison to the control diet and the FO-supplemented diet without/with CA.

The consumption of chicken meat has steadily increased in recent decades, thereby making meat enriched with long-chain polyunsaturated fatty acids (LC-PUFA) an important delivery route of this nutrient to humans. This study aimed to evaluate how feeding duration of a diet with fish oil and flaxseed or rapeseed can enhance broiler meat with n-3 fatty acids (FA). One hundred and twenty-three 15-day-old female broilers Ross 308 were housed individually and randomly allocated to four groups: control (15 birds), and three experimental groups (36 birds each) that were each divided into three subgroups of 12 birds. Four diets differing in fat composition were prepared. Lard was the source of supplementary fat in diet L; in the experimental diets, fat was derived from fish oil (FO, 10 g kg−1 diet) and either lard (LFO), flaxseed (FFO), or rapeseed (RFO) in amounts appropriate to produce fat levels similar to those in the control diet. Diets were cold-pelleted and fed until slaughter at 36 days of age. The control group was fed the L diet throughout the study, whereas birds in the experimental groups were fed the L diet until it was substituted by the experimental diets for one, two, or three weeks preceding slaughter. The performance of all groups did not differ from the control, but within the experimental groups, LFO birds had a higher body weight gain because of higher feed intake. RFO birds had a higher thyroid weight (P

Consumption of chicken meat enriched with bioactive compounds such as n-3 polyunsaturated fatty acids (PUFAn-3), vitamin E (vE) and selenium (Se) can help prevent many diseases and can be used to deliver those substances to humans. This might be of importance as chicken meat consumption is increasing worldwide. The effects of enriching chicken meat with PUFAn-3, vE and Se through dietary interventions were studied in rats. Four groups of Ross 308 female broilers from day 22 to day 35 of age were fed control diet (L) that contained lard and 80 mg vE and 0.3 mg Se/kg, or diets that contained rape seeds and fish oil with the same level of Se and vE as in the control diet, the same level of Se as in the control and 150 mg vE/kg, or 150 mg of vE and 0.7 mg Se/kg. Broiler carcasses were boiled, deboned, lyophilized and pooled by group. Boiled edible components of chicken carcass (BECC) were included (240 g/kg) in the diets fed to four groups of ten 10-week-old Wistar male rats for 8 weeks. Inclusion of BECCs modulated dietary fatty acid profile in the rat diets. Feeding these diets did not influence parameters related to growth or relative weights of internal organs in the rats. Feeding BECCs with lower PUFAn-6/n-3 decreased the n-6/n-3 ratio in the rat brain and liver, and increased the proportion of docosahexaenoic acid in the brain lipids. Liver cholesterol level was similar among the experimental groups, whereas the concentration of vE in the liver of rats fed BECC with increased vE levels was higher than that in the rats fed BECC with the basal vE level. Haematological and biochemical parameters in blood were within the normal range for rats, but a few rats showed a tendency towards increased levels because of the higher vE and Se level. The health-promoting effect of feeding rats PUFAn-3 enriched BECC was more pronounced when an increased dietary level of vE was used, but the increased level of Se did not provide the rats with additional benefits. Thus, the findings indicate that BECC enriched with PUFAn-3 and vE by a dietary intervention is a functional food with great potential of implementation.

The objective of the present study was to determine the influence of dietary supplementation with pomegranate seed oil (PSO) and/or an aqueous extract of dried bitter melon fruits (BME) on breast cancer risk and fatty acid profile in serum of female rats with chemical carcinogen-inflicted mammary tumours. Sprague-Dawley rats (n = 96) were fed control diet or experimental diets supplemented with 0.15 ml PSO/day, BME or jointly PSO and BME. After 21 weeks mammary tumours were subjected to histopathological examination and in serum fatty acids, 8-isoprostaglandin F2α content and indices of desaturases activity were analysed. Supplementation of the diet with PSO and BME did not inhibit the breast cancer formation. Conjugated linolenic acids (CLnA), present in PSO, were converted into cis-9, trans-11 conjugated linoleic acid (CLA), however, its content was lower in groups treated with a carcinogen. A similar tendency was observed for the content of SFA, MUFA, PUFA, 8-iso PGF2α and the activity of Δ6-desaturase. Enhanced pro-carcinogenic effect of 7,12-dimethylbenz[a]anthracene (DMBA), caused by applied supplements, may be a result of their influence on DMBA metabolism.

Despite promising health effects of pomegranate seed oil (PSO) and bitter melon extract (BM) used for centuries as food and traditional medicine, neither mechanism of action nor safety has been fully recognized. This study aimed to evaluate the influence of diet supplementation with PSO and BM on fatty acid, conjugated fatty acid and cholesterol content in rat' livers, since liver is crucial for lipid metabolism. Oxidation indicators (malondialdehyde, oxysterols and tocopherols) were also determined. Lipid profiles did not reveal the presence of punicic acid, while other conjugated dienes and trienes, including rumenic acid, were determined. Both supplementation and exposition to carcinogen significantly increased cholesterol and reduced selected oxysterols levels, simultaneously increasing malondialdehyde content in animals suffering from cancer. Impact of PSO and BM on oxidative status varied depending on carcinogen exposure and coexisting neoplastic process, which is important, due to the growing interest in their use in prevention and therapy of various diseases, including cancer.

The aim of the study was to evaluate longissimus muscle quality in lambs fed diets including fish oil (FO), rapeseed oil (RO), carnosic acid (CA) and seleno-compounds. Lambs were fed one of diets: Group I - the basal diet (BD) with 3% RO; Group II - BD with 2% RO and 1% FO; Group III - BD with 2% RO, 1% FO and 0.1% CA; Group IV - BD with 2% RO, 1% FO, 0.1% CA and 0.35ppm Se as selenized-yeast; Group V - BD with 2% RO, 1% FO, 0.1% CA and 0.35ppm Se as selenate. The addition of FO and FO, CA and selenium in the inorganic form was characterized by lowest tenderness and juiciness (P

Lambs were divided into 3 groups of 6 animals each. For 35 days lambs were fed a diet including 2% rapeseed oil, 1% fish oil and 0.1% carnosic acid (the control group) or two experimental diets supplemented with 0.35 mg ∙ kg−1Se as selenized-yeast (SeY) (the SeY diet) or selenate (the selenate diet). Muscles (Musculus longissimus dorsi(MLD) andMusculus biceps femoris(MBF)), ruminal fluids and microbiota were collected from each lamb. SeY supplementation most effectively stimulated the accumulation of straight-chain volatile fatty acids (VFAs),iso-branched-chain VFAs, CO2and CH4in the ruminal fluid. The contents of CO2, CH4and VFAs including straight-chain VFAs with the exception ofiso-branched-chain VFAs were most effectively reduced by the selenite diet. The control diet most efficiently increased the concentration sums ofodd-saturated fatty acids (odd-SFAs) andiso-SFAs in microbiota. The SeY diet most efficiently reduced acetic acid to propionic acid ratio in the ruminal fluid. The selenate diet improved animal performance by reducing ruminal concentrations of CH4and CO2. The SeY diet and especially the selenate diet reduced the biohydrogenation to C18:0 when compared with the control diet. The selenate diet more efficiently reduced the concentration sums of all SFAs (ΣSFAs) and all fatty acids (ΣFAs) inMLDandMBFthan the SeY diet, which most effectively increased the concentrations of ΣSFAs and ΣFAs inMLDandMBF. The selenate diet most effectively increased the body mass gain of lambs.

The main aim of our study was to investigate the influence of selenite (SeIV) or selenate (SeVI) added to ovine ruminal fluid with cis9cis12cis15C18:3 (LNA) on contents of fatty acids (FA), especially products of microbial isomerisation of LNA and biohydrogenation (BH) of unsaturated FA in vitro incubated fluids. The second goal of our study was to investigate the effects of SeVI added to a diet containing linseed oil (LO, rich in LNA) on the accumulation of BH products in selected tissues of lambs. Ruminal fluids were incubated in vitro at 39°C under CO2 either alone (the control fluid; RF) or with LNA (1.67 mg/ml), a low (1.33 μg/ml), a high (3.33 μg/ml) dose of Se (as SeIV or SeVI), with a combination of LNA with either a low (1.33 μg/ml) or a high (3.33 μg/ml) dose of Se as SeIV or SeVI. The tubes with incubated fluids were removed after 0, 6, 12, 18 or 24 h of in vitro incubation and then analysed to determine contents of FA. Both the doses of SeIV or SeVI in fluids with LNA revealed no significant effect on the contents of products of LNA isomerisation compared to incubated fluids with LNA. The contents of isomerisation products of LNA in incubated fluids with LNA, irrespective of the presence of SeIV and SeVI, rapidly increased in the fluids incubated for 6 h, followed by a slower rate of disappearance until 24 h. Contents of trans11C18:1 and trans11cis15C18:2 in fluids with LNA, regardless of the presence of both the doses of SeIV or SeVI, increased throughout incubations. The lower dose of SeVI in fluids with LNA increased the capacity of BH to trans11C18:1 in incubated fluids. SeVI added to the lambs’ diet with LO decreased contents of saturated FA (like C18:0) in the liver and musculus longissimus dorsi. LO added to the diets with or without SeVI most effectively increased the level of polyunsaturated FA (PUFA) in the liver, while the higher content of monounsaturated FA was observed in musculus biceps fermoris (MBF) when compared to the control group. The diets with LO, irrespective of the presence of SeVI, reduced the capacity of linoleic acid elongation to cis11cis14C20:2 in the liver and MBF compared to the control lambs. Further studies are required to clarify the effects of SeVI and long-chain PUFA on contents of FA, especially conjugated PUFA, in the ruminal microbiota and tissues of lambs.

Research on pregnant mice and rats indicated that a neurosteroid allopregnanolone (AP) might have a stress-protective effect, decreasing the activity of the hypothalamic-pituitary-adrenal (HPA) axis. Using a sheep model in the present research, we tested a hypothesis that the cerebrospinal fluid (CSF) AP concentration changes during pregnancy and early lactation, keeping in check the secretory activity of the HPA axis. Four-hour-long CSF collections (20 μL/min, from 10:00 to 14:00) from the third brain ventricle were conducted in 8 sheep, every 4 weeks, starting from the 28th day of pregnancy until the 28th day after delivery, to determine AP and corticotropin-releasing hormone (CRH) concentrations. A maximum of 8 samples (every 30 min) were gathered from individual sheep. Each series of CSF collections was accompanied by blood sampling every 10 min, to examine the distribution of mean plasma adrenocorticotropin (ACTH) and cortisol concentrations. Six luteal-phase sheep were used as a control during the period corresponding to the first month of pregnancy. The highest AP concentration was noted in the 5th month of pregnancy compared to these in the 1st and 2nd months (P