Back to Search Start Over

Label-Free Mass Spectrometry-Based Proteomic Analysis in Lamb Tissues after Fish Oil, Carnosic Acid, and Inorganic Selenium Supplementation

Authors :
Andrzej Gawor
Anna Ruszczyńska
Anna Konopka
Grzegorz Wryk
Marian Czauderna
Ewa Bulska
Source :
Animals, Vol 12, Iss 11, p 1428 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Selenium is an essential nutrient, building twenty five identified selenoproteins in humans known to perform several important biological functions. The small amount of selenium in the earth’s crust in certain regions along with the risk of deficiency in organisms have resulted in increasingly popular dietary supplementation in animals, implemented via, e.g., inorganic selenium compounds. Even though selenium is included in selenoproteins in the form of selenocysteine, the dietary effect of selenium may result in the expression of other proteins or genes. Very little is known about the expression effects modulated by selenium. The present study aimed to examine the significance of protein expression in lamb tissues obtained after dietary supplementation with selenium (sodium selenate) and two other feed additives, fish oil and carnosic acid. Label-free mass spectrometry-based proteomic analysis was successfully applied to examine the animal tissues. Protein-protein interaction network analysis of forty differently-expressed proteins following inorganic selenium supplementation indicated two significant clusters which are involved in cell adhesion, heart development, actin filament-based movement, plasma membrane repair, and establishment of organelle localization.

Details

Language :
English
ISSN :
20762615
Volume :
12
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.23332cca8254c7d8b2b846b6206cb2b
Document Type :
article
Full Text :
https://doi.org/10.3390/ani12111428