18 results on '"Maria Lucia Iacovino"'
Search Results
2. Circulating vitamin D level before initiating chemotherapy impacts on the time-to-outcome in metastatic colorectal cancer patients: systematic review and meta-analysis
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Alessandro Ottaiano, Maria Lucia Iacovino, Mariachiara Santorsola, Sergio Facchini, Domenico Iervolino, Francesco Perri, Guglielmo Nasti, Vincenzo Quagliariello, Nicola Maurea, Andrea Ronchi, Bianca Arianna Facchini, Alessia Bignucolo, and Massimiliano Berretta
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Metastatic colorectal cancer ,Vitamin D ,Prognosis ,Meta-analysis ,Overall survival ,Progression-free survival ,Medicine - Abstract
Abstract Background Vitamin D (VD) is implicated in various health conditions, including colorectal cancer (CRC). To investigate potential relationships between pre-chemotherapy VD levels and the time-to-outcome in metastatic CRC patients, we conducted a systematic review and meta-analysis. Methods Following the PRISMA 2020 guidelines, we performed thorough searches in PubMed/MEDLINE and Scopus/ELSEVIER databases (covering the years 2002 to 2022). Inclusion criteria mandated studies to report on individuals aged 18 years and above with histologically confirmed stage IV CRC. Additionally, studies needed to provide data on VD levels before chemotherapy, along with hazard ratios (HR) and 95% confidence intervals (CIs) for overall survival (OS) and/or progression-free survival (PFS). Five articles were identified with the aim of establishing a combined risk estimate for death and progression based on pre-chemotherapy VD levels. Heterogeneity among studies and publication bias were evaluated using Tau2, I2 statistics, and a Funnel plot. Results Although no significant heterogeneity was observed in time-to-outcome among the selected studies, variations in technical assessments and serum VD concentration measurements were noted. The pooled analysis, involving 1712 patients for OS and 1264 patients for PFS, revealed a 47% increased risk of death (HR: 1.47, 95% CI: 1.21–1.79) and a 38% increased risk of progression (HR: 1.38, 95% CI: 1.13–1.70) for patients with lower VD levels, as indicated by fixed-effects models. Conclusions Our results emphasize the adverse effects of low VD concentration on the time-to-outcome in metastatic CRC patients. This underscores the importance of investigating VD supplementation as an innovative approach in this clinical setting to enhance patient outcomes.
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- 2024
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3. Asymptomatic azygos vein overflow in a young patient with primary mediastinal seminoma
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Raimondo Di Liello, Francesca Sparano, Maria Lucia Iacovino, Giuseppe Viscardi, Carminia Maria Della Corte, Andrea Ronchi, Rosa Laura Sabetta, Morena Fasano, Giovanni Vicidomini, Alfonso Reginelli, Fortunato Ciardiello, and Floriana Morgillo
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Primary mediastinal seminoma ,radiology ,superior vena cava syndrome ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The azygos system is the most important pathway for decompression of the superior vena cava (SVC) when a blood flow obstruction to the right atrium is present. Thoracic and mediastinal malignancies, mainly lung cancers, are responsible for 60%–85% of superior vena cava syndrome (SVCS) cases. An uncommon origin of SVCS is primary malignant mediastinal germ cell tumor (PMMGCT) which represent 1%–4% of all mediastinal tumors and can be divided into two broad groups: seminomas and nonseminomatous germ cell tumors (NSGCTs). Primary mediastinal seminomas clinical presentation is often nonspecific, even if the majority of patients present with superior vena cava involvement. Here, we present the radiologic features of asymptomatic azygos system overflow in a patient with primary mediastinal seminoma.
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- 2019
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4. Head and neck cancer: the role of anti-EGFR agents in the era of immunotherapy
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Morena Fasano, Carminia Maria Della Corte, Giuseppe Viscardi, Raimondo Di Liello, Fernando Paragliola, Francesca Sparano, Maria Lucia Iacovino, Anna Castrichino, Francesca Doria, Antonello Sica, Floriana Morgillo, Giuseppe Colella, Giampaolo Tartaro, Salvatore Cappabianca, Domenico Testa, Gaetano Motta, and Fortunato Ciardiello
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Head and neck cancers (HNC) represent the seventh most frequent cancer worldwide, with squamous cell carcinomas as the most frequent histologic subtype. Standard treatment for early stage diseases is represented by single modality surgery or radiotherapy, whereas in the locally advanced and recurrent or metastatic settings a more aggressive multi-modal approach is needed with locoregional intervention and/or systemic therapies. Epidermal Growth Factor Receptor (EGFR) plays an important role in HNC biology and has been studied extensively in preclinical and clinical settings. In this scenario, anti-EGFR targeted agent cetuximab, introduced in clinical practice a decade ago, represents the only approved targeted therapy to date, while the development of immune-checkpoint inhibitors has recently changed the available treatment options. In this review, we focus on the current role of anti-EGFR therapies in HNCs, underlying available clinical data and mechanisms of resistance, and highlight future perspectives regarding their role in the era of immunotherapy.
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- 2021
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5. Induction of natural killer antibody-dependent cell cytotoxicity and of clinical activity of cetuximab plus avelumab in non-small cell lung cancer
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Fortunato Ciardiello, Carminia Maria Della Corte, Floriana Morgillo, Vincenzo Sforza, Raimondo Di Liello, Vincenza Ciaramella, Giusi Barra, Francesca Sparano, Giuseppe Viscardi, Maria Lucia Iacovino, Morena Fasano, Vincenzo Famiglietti, Evaristo Maiello, Fernando Paragliola, Flora Cimmino, Mario Capasso, Achille Iolascon, and Alessandro Morabito
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background Antibody-dependent cell-mediated cytotoxicity (ADCC) may mediate antitumour activity of IgG1-isotype monoclonal antibody (mAb), suggesting as potential treatment combination of IgG1-mAbs, anti-epidermal growth factor receptor cetuximab and anti-programmed death-ligand-1 avelumab.Methods We evaluated ADCC induction in lung cancer cells by lactate dehydrogenase (LDH) release assay. Antitumour activity and safety of cetuximab plus avelumab were explored in a single-arm proof-of-concept study in pre-treated non-small cell lung cancer (NSCLC) patients (pt) (Cetuximab-AVElumab-lung, CAVE-Lung). Search for predictive biomarkers of response was done.Results Avelumab plus cetuximab induced ADCC in NSCLC cells in vitro in presence of natural killers (NK) from healthy donors (HD) or NSCLC pt, as effectors. Sixteen relapsed NSCLC pt were treated with avelumab plus cetuximab. Antitumour activity was observed in 6/16 pt, defined by progression free survival (PFS) ≥8 months, with 4 of them still on treatment at data lock time (range, 14–19 months). Of note, 3/6 responders had received as previous line anti-programmed death-1 therapy. In responders, clinical benefit was accompanied by significant increase in LDH release over baseline at the first radiological evaluation (8 weeks) (p=0.01) and by early skin toxicity; while in the 10 non-responders, that had PFS ≤5 months, LDH release tends to reduce. Baseline circulating DNA levels were higher in non-responders compared with responders and HD (p=0.026) and decrease in responders during therapy. Mutations in DNA damage responsive family genes were found in responders.Conclusion Cetuximab and avelumab activates NSCLC pt NK cells. Ex vivo evaluation of ADCC, circulating DNA levels and early skin toxicity may predict response to cetuximab plus avelumab in NSCLC.EUDRACT 2017-004195-58
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- 2020
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6. Novel Therapeutic Opportunities in Neoadjuvant Setting in Urothelial Cancers: A New Horizon Opened by Molecular Classification and Immune Checkpoint Inhibitors
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Maria Lucia Iacovino, Chiara Carmen Miceli, Marco De Felice, Biagio Barone, Luca Pompella, Francesco Chiancone, Erika Di Zazzo, Giuseppe Tirino, Carminia Maria Della Corte, Ciro Imbimbo, Ferdinando De Vita, and Felice Crocetto
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bladder cancer ,urothelial cancers ,neoadjuvant chemotherapy ,Immune Checkpoint Inhibitors ,molecular classification of muscle-invasive bladder cancer ,muscle-invasive bladder cancer ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Muscle invasive bladder cancer (MIBC) is a widespread malignancy with a worse prognosis often related to a late diagnosis. For early-stage MIBC pts, a multidisciplinary approach is mandatory to evaluate the timing of neoadjuvant chemotherapy (NAC) and surgery. The current standard therapy is platinum-based NAC (MVAC-methotrexate, vinblastine, doxorubicin, and cisplatin or Platinum–Gemcitabine regimens) followed by radical cystectomy (RC) with lymphadenectomy. However, preliminary data from Vesper trial highlighted that dose-dense NAC MVAC is endowed with a good pathological response but shows low tolerability. In the last few years, translational-based research approaches have identified several candidate biomarkers of NAC esponsiveness, such as ERCC2, ERBB2, or DNA damage response (DDR) gene alterations. Moreover, the recent consensus MIBC molecular classification identified six molecular subtypes, characterized by different sensitivity to chemo- or targeted or immunotherapy, that could open a novel procedure for patient selection and also for neoadjuvant therapies. The Italian PURE-01 phase II Trial extended data on efficacy and resistance to Immune Checkpoint Inhibitors (ICIs) in this setting. In this review, we summarize the most relevant literature data supporting NAC use in MIBC, focusing on novel therapeutic strategies such as immunotherapy, considering the better patient stratification and selection emerging from novel molecular classification.
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- 2022
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7. Ex vivo lung cancer spheroids resemble treatment response of a patient with NSCLC to chemotherapy and immunotherapy: case report and translational study
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Carminia Maria Della Corte, Federica Papaccio, Vincenza Ciaramella, Giusi Barra, Massimo Venditti, Francesca Sparano, Maria Lucia Iacovino, and Sergio Minucci
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Introduction In the era of precision medicine, research studies are aiming to design patient-tailored treatment strategies. In this work, we present a clinical case of a patient with non-small cell lung cancer (NSCLC) accompanied by a translational study with the intent to assess the correspondence of drug sensitivity in ex vivo spheroidal tumour cultures and peripheral blood biomarkers with clinical outcome.Methods Primary tumour tissue, patient-derived tumour spheroids, peripheral blood mononuclear cells and circulating DNA were analysed to assess drug sensitivity and immunological profiling, and all these data were correlated with clinical and radiological evaluations.Results Immunohistochemistry, immunofluorescence, next generation sequencing analysis and T-lymphocyte receptor repertoire assay results showed elevated concordance among primary tumour tissue, ex vivo three-dimensional tumour spheroid specimen and circulating DNA. Cisplatin-based chemotherapy and anti-programmed death 1 drug sensitivity assessed in spheroidal cultures were strictly consistent with patient clinical response to adjuvant chemotherapy and first-line immune therapy.Conclusion These results revealed that ex vivo drug sensitivity testing in three-dimensional spheroidal culture can reproduce clinical response to chemotherapy and immunotherapy, with the potential to use those culture models to predict patients‘ outcome from anticancer treatments and, therefore, the feasibility to select individualised therapy.
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- 2019
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8. Novel Therapeutic Opportunities in Neoadjuvant Setting in Urothelial Cancers: A New Horizon Opened by Molecular Classification and Immune Checkpoint Inhibitors
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Maria Lucia Iacovino, Chiara Carmen Miceli, Marco De Felice, Biagio Barone, Luca Pompella, Francesco Chiancone, Erika Di Zazzo, Giuseppe Tirino, Carminia Maria Della Corte, Ciro Imbimbo, Ferdinando De Vita, Felice Crocetto, Iacovino, M. L., Miceli, C. C., De Felice, M., Barone, B., Pompella, L., Chiancone, F., Di Zazzo, E., Tirino, G., Della Corte, C. M., Imbimbo, C., De Vita, F., and Crocetto, F.
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QH301-705.5 ,Immune Checkpoint Inhibitor ,Urothelial cancers ,Cystectomy ,Neoadjuvant chemotherapy ,Catalysis ,Inorganic Chemistry ,Molecular classification of muscle-invasive bladder cancer ,Antineoplastic Combined Chemotherapy Protocols ,Urothelial cancer ,Biomarkers, Tumor ,Humans ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Immune Checkpoint Inhibitors ,Spectroscopy ,Carcinoma, Transitional Cell ,Clinical Trials as Topic ,Antineoplastic Combined Chemotherapy Protocol ,Organic Chemistry ,Bladder cancer ,General Medicine ,Neoadjuvant Therapy ,Computer Science Applications ,Gene Expression Regulation, Neoplastic ,Chemistry ,Urinary Bladder Neoplasms ,Lymph Node Excision ,Human ,Muscle-invasive bladder cancer - Abstract
Muscle invasive bladder cancer (MIBC) is a widespread malignancy with a worse prognosis often related to a late diagnosis. For early-stage MIBC pts, a multidisciplinary approach is mandatory to evaluate the timing of neoadjuvant chemotherapy (NAC) and surgery. The current standard therapy is platinum-based NAC (MVAC-methotrexate, vinblastine, doxorubicin, and cisplatin or Platinum–Gemcitabine regimens) followed by radical cystectomy (RC) with lymphadenectomy. However, preliminary data from Vesper trial highlighted that dose-dense NAC MVAC is endowed with a good pathological response but shows low tolerability. In the last few years, translational-based research approaches have identified several candidate biomarkers of NAC esponsiveness, such as ERCC2, ERBB2, or DNA damage response (DDR) gene alterations. Moreover, the recent consensus MIBC molecular classification identified six molecular subtypes, characterized by different sensitivity to chemo- or targeted or immunotherapy, that could open a novel procedure for patient selection and also for neoadjuvant therapies. The Italian PURE-01 phase II Trial extended data on efficacy and resistance to Immune Checkpoint Inhibitors (ICIs) in this setting. In this review, we summarize the most relevant literature data supporting NAC use in MIBC, focusing on novel therapeutic strategies such as immunotherapy, considering the better patient stratification and selection emerging from novel molecular classification.
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- 2021
9. Asymptomatic azygos vein overflow in a young patient with primary mediastinal seminoma
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Andrea Ronchi, Morena Fasano, Giuseppe Viscardi, Maria Lucia Iacovino, Francesca Sparano, Rosa Laura Sabetta, Floriana Morgillo, Giovanni Vicidomini, Alfonso Reginelli, Fortunato Ciardiello, Carminia Maria Della Corte, Raimondo Di Liello, Di Liello, Raimondo, Sparano, Francesca, Iacovino, Maria Lucia, Viscardi, Giuseppe, Della Corte, Carminia Maria, Ronchi, Andrea, Sabetta, Rosa Laura, Fasano, Morena, Vicidomini, Giovanni, Reginelli, Alfonso, Ciardiello, Fortunato, and Morgillo, Floriana
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Mediastinal germ cell tumor ,Case Report ,Case Reports ,lcsh:RC254-282 ,Asymptomatic ,03 medical and health sciences ,0302 clinical medicine ,Superior vena cava ,medicine ,cardiovascular diseases ,Primary mediastinal seminoma ,Lung ,Superior vena cava syndrome ,Mediastinal Seminoma ,business.industry ,superior vena cava syndrome ,General Medicine ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,radiology ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,cardiovascular system ,Radiology ,Germ cell tumors ,medicine.symptom ,Azygos vein ,business - Abstract
The azygos system is the most important pathway for decompression of the superior vena cava (SVC) when a blood flow obstruction to the right atrium is present. Thoracic and mediastinal malignancies, mainly lung cancers, are responsible for 60%-85% of superior vena cava syndrome (SVCS) cases. An uncommon origin of SVCS is primary malignant mediastinal germ cell tumor (PMMGCT) which represent 1%-4% of all mediastinal tumors and can be divided into two broad groups: seminomas and nonseminomatous germ cell tumors (NSGCTs). Primary mediastinal seminomas clinical presentation is often nonspecific, even if the majority of patients present with superior vena cava involvement. Here, we present the radiologic features of asymptomatic azygos system overflow in a patient with primary mediastinal seminoma.
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- 2019
10. Head and neck cancer: the role of anti-EGFR agents in the era of immunotherapy
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Floriana Morgillo, Carminia Maria Della Corte, Salvatore Cappabianca, Raimondo Di Liello, Francesca Sparano, Domenico Testa, Francesca Doria, Giuseppe Viscardi, Anna Castrichino, Maria Lucia Iacovino, Fernando Paragliola, Fortunato Ciardiello, Giuseppe Colella, Antonello Sica, Morena Fasano, Gaetano Motta, Giampaolo Tartaro, Fasano, M., Della Corte, C. M., Viscardi, G., Di Liello, R., Paragliola, F., Sparano, F., Iacovino, M. L., Castrichino, A., Doria, F., Sica, A., Morgillo, F., Colella, G., Tartaro, G., Cappabianca, S., Testa, D., Motta, G., and Ciardiello, F.
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Oncology ,medicine.medical_specialty ,medicine.medical_treatment ,EGFR ,Review ,lcsh:RC254-282 ,HNSCC ,Targeted therapy ,Internal medicine ,cetuximab ,medicine ,Epidermal growth factor receptor ,Cetuximab ,biology ,business.industry ,Standard treatment ,Head and neck cancer ,Cancer ,Immunotherapy ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Radiation therapy ,HNC ,biology.protein ,immunotherapy ,business ,medicine.drug - Abstract
Head and neck cancers (HNC) represent the seventh most frequent cancer worldwide, with squamous cell carcinomas as the most frequent histologic subtype. Standard treatment for early stage diseases is represented by single modality surgery or radiotherapy, whereas in the locally advanced and recurrent or metastatic settings a more aggressive multi-modal approach is needed with locoregional intervention and/or systemic therapies. Epidermal Growth Factor Receptor (EGFR) plays an important role in HNC biology and has been studied extensively in preclinical and clinical settings. In this scenario, anti-EGFR targeted agent cetuximab, introduced in clinical practice a decade ago, represents the only approved targeted therapy to date, while the development of immune-checkpoint inhibitors has recently changed the available treatment options. In this review, we focus on the current role of anti-EGFR therapies in HNCs, underlying available clinical data and mechanisms of resistance, and highlight future perspectives regarding their role in the era of immunotherapy.
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- 2021
11. Immunotherapy in advanced Non-Small Cell Lung Cancer patients with poor performance status: The role of clinical-pathological variables and inflammatory biomarkers
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Diego Signorelli, A. Prelaj, Benedetta Trevisan, Giacomo Massa, Giulia Galli, Marina Chiara Garassino, Carminia Maria Della Corte, Claudia Proto, Floriana Morgillo, Francesca Sparano, Giuseppe Lo Russo, Filippo de Braud, Raimondo Di Liello, Fortunato Ciardiello, Giuseppe Viscardi, Marta Brambilla, Maria Lucia Iacovino, Alessandro De Toma, Monica Ganzinelli, Roberto Ferrara, Riccardo Lobefaro, Lobefaro, R., Viscardi, G., Di Liello, R., Massa, G., Iacovino, M. L., Sparano, F., Della Corte, C. M., Ferrara, R., Signorelli, D., Proto, C., Prelaj, A., Galli, G., De Toma, A., Brambilla, M., Ganzinelli, M., Trevisan, B., Ciardiello, F., De Braud, F., Morgillo, F., Garassino, M. C., and Lo Russo, G.
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,Oncology ,Unfit ,Male ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,medicine.medical_treatment ,ECOG Performance Status ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Randomized controlled trial ,law ,Retrospective Studie ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Humans ,Progression-free survival ,Lung cancer ,Pathological ,Poor performance statu ,Poor performance status ,Retrospective Studies ,business.industry ,Immunotherapy ,Biomarker ,medicine.disease ,Non-Small Cell Lung Cancer ,030104 developmental biology ,030220 oncology & carcinogenesis ,Patient survival ,Female ,Non small cell ,Safety ,business ,Biomarkers ,Human - Abstract
Background: The introduction of immunotherapy has improved the prognosis of patients with Non-Small Cell Lung Cancer (NSCLC). However, data in poor ECOG Performance Status (PS) patients remain scant due to their exclusion from randomized trials. Material and methods: We analyzed data of patients with advanced NSCLC treated with immunotherapy in two Italian Centers, to evaluate the impact of PS (0-1 vs 2) on disease control rate (DCR), progression free survival (PFS) and overall survival (OS). Chi-square test was used to compare clinical-pathological variables, their impact on survival was evaluated through Cox proportional hazard models. Results: Among 404 patients included, PS was 0 in 137 (33.9 %), 1 in 208 (51.5 %) and 2 in 59 (14.6 %) patients; 143 were female and 90 had squamous NSCLC. Clinical-pathological variables were uniformly distributed except for higher prevalence of liver metastases in patients with poor PS. We found that PS2 patients showed worse outcomes in terms of DCR (21.8 % vs 50.3 %, p = 0.001), PFS [2.0 (95 % CI 1.6–3.0) vs 3.0 (95 % CI 2.7–4.0) months, p < 0.0001] and OS [4.0 (95 % CI 2.8–5.7) vs 13.2 (95 % CI 11.0−15.8) months, p < 0.0001]. PS2 status, negative PDL1 expression and early corticosteroids exposure as well as higher Neutrophil to Lymphocyte Ratio and LDH at baseline were associated with worse outcomes at univariate and multivariable analysis. Subgroup analysis confirmed poor outcomes in PS2 patients with high LDH and concomitant corticosteroid therapies. The incidence of Grade 3/4 adverse events was 11.3 % in PS 0−1 and 10.2 % in PS 2 patients (p = 0.81). Conclusion: Our data confirm reduced efficacy of immunotherapy in patients with poor PS even though a good safety. Despite PS remains the most powerful independent prognostic factor for NSCLC, LDH levels and steroids exposure could support the decision making in PS2 patients.
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- 2020
12. Abstract PO-052: A pilot study of miRNA expression profile in surgically resected pancreatic ductal adenocarcinoma: Initial report from a bi-institutional cohort
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Giovanni Conzo, Francesca Cardella, Michele Caraglia, Annamaria Auricchio, Luca Pompella, Severo Campione, Gabriella Misso, Francesca Sparano, Anna Grimaldi, Chiara Carmen Miceli, Angela Lombardi, Renato Franco, Marco Montella, Gennaro Galizia, Ferdinando De Vita, Maria Lucia Iacovino, Vincenzo Napolitano, Carlo Molino, Fortunato Ciardiello, Michela Falco, Giuseppe Tirino, and Carlo Caputo
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Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,Microarray analysis techniques ,In silico ,Cancer ,Context (language use) ,Disease ,medicine.disease ,Pancreatic cancer ,Internal medicine ,microRNA ,Cohort ,Medicine ,business - Abstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human malignancies: novel therapeutic approaches beyond conventional chemotherapy are still lacking and prognosis remains poor, even for resectable patients (pts). Furthermore, there is an almost complete absence of validated predictive factors. Consequently, robust biomarkers for the early diagnosis and the prognostic stratification are urgently needed in clinical practice, especially in the context of neoadjuvant and adjuvant settings. In the last years, evidence revealed the crucial role of miRNAs in cancer initiation and progression, as well as in the chemo-resistance mechanisms, suggesting their use as clinical biomarkers. Material and methods: In this pilot study, we performed a microarray analysis to characterize global miRNA expression profile from surgical tissue samples collected from 20 resected PDAC pts pooled into 4 groups according to different clinico-pathological features: nodal metastases (N+/N-) and tumor grading (G2/G3). Results: According to expression patterns, we identified, among 384 miRNAs, a significant different modulation for 11 miRNAs associated to G2 vs G3 and for 7 miRNAs in N+ vs N- disease, suggesting a possible specific signature reflecting histological grade and nodal metastasis occurrence, respectively. We focused on 2 up-regulated (miR-138-5p and miR-518-3p) and 3 down-regulated (miR-215-5p, miR-519a-3p and miR-576-5p) miRNAs in N+ pts, and on 3 up-regulated (miR-1-3p, miR-31-5p and miR-205-5p) in G3 pts, in order to verify their possible implication in the molecular changes behind tumor differentiation and spread, as well as their potential use for prognostic and therapeutic purpose. A bio-informatic analysis was also performed, using different in silico tools, to study both high affinity miRNA targets and cross-regulated pathways among the upand down-regulated miRNAs. The results identified several associated targets involved in multiple signaling pathways commonly dysregulated in cancer. Finally, BRCA1/2 and RB1 miRNAs-mediated-modulation is actually ongoing, considering the pivotal role of these genes in some PDAC pts. Conclusion: These preliminary data provide a strong rationale to further investigate miRNAs expression in larger cohorts of PDAC pts, possibly integrating validated tissue miRNAs data with circulating miRNAs, in order to identify strong (and easily accessible) potential biomarker(s) with prognostic and/or predictive significance. Citation Format: Luca Pompella, Michela Falco, Carlo Caputo, Anna Grimaldi, Giuseppe Tirino, Severo Campione, Francesca Sparano, Maria Lucia Iacovino, Chiara Carmen Miceli, Carlo Molino, Marco Montella, Renato Franco, Gennaro Galizia, Giovanni Conzo, Vincenzo Napolitano, Annamaria Auricchio, Francesca Cardella, Fortunato Ciardiello, Michele Caraglia, Angela Lombardi, Gabriella Misso, Ferdinando De Vita. A pilot study of miRNA expression profile in surgically resected pancreatic ductal adenocarcinoma: Initial report from a bi-institutional cohort [abstract]. In: Proceedings of the AACR Virtual Special Conference on Pancreatic Cancer; 2021 Sep 29-30. Philadelphia (PA): AACR; Cancer Res 2021;81(22 Suppl):Abstract nr PO-052.
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- 2021
13. 1329P Immune checkpoint inhibitors in advanced NSCLC patients with poor performance status: The role of clinical-pathological variables and inflammatory biomarkers in a real world experience
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Roberto Ferrara, C.M. Della Corte, Floriana Morgillo, Giulia Galli, Francesca Sparano, R. Di Liello, Marina Chiara Garassino, Monica Ganzinelli, Arsela Prelaj, Giuseppe Viscardi, Marta Brambilla, G. Lo Russo, Riccardo Lobefaro, Claudia Proto, Benedetta Trevisan, Diego Signorelli, A. De Toma, Maria Lucia Iacovino, Giacomo Massa, and F. de Braud
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Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,Immune checkpoint inhibitors ,medicine ,Poor performance status ,Hematology ,business ,Pathological ,Inflammatory biomarkers - Published
- 2020
14. 1335P Anti-tumour efficacy of cetuximab plus avelumab in NSCLC through induction of ADCC: Final data from CAVE-lung trial
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Floriana Morgillo, Morena Fasano, C.M. Della Corte, Alessandro Morabito, Evaristo Maiello, Francesca Sparano, Maria Lucia Iacovino, Fortunato Ciardiello, Vincenza Ciaramella, Fernando Paragliola, Giuseppe Viscardi, Vincenzo Famiglietti, Giusi Barra, Vincenzo Sforza, Flora Cimmino, and R. Di Liello
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Antibody-dependent cell-mediated cytotoxicity ,geography ,Lung ,geography.geographical_feature_category ,Cetuximab ,business.industry ,Hematology ,Avelumab ,Anti tumour ,medicine.anatomical_structure ,Oncology ,Cave ,medicine ,Cancer research ,business ,medicine.drug - Published
- 2020
15. Efficacy and safety of immunotherapy in non-small cell lung cancer patients with poor performance status
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Marina Chiara Garassino, Giulia Galli, Claudia Proto, A. Bottiglieri, Diego Signorelli, Giacomo Massa, Benedetta Trevisan, Giuseppe Viscardi, Alessandro De Toma, Floriana Morgillo, Riccardo Lobefaro, Arsela Prelaj, Giuseppe Lo Russo, Maria Lucia Iacovino, Filippo de Braud, Marta Brambilla, Monica Ganzinelli, Francesca Sparano, Raimondo Di Liello, and Roberto Ferrara
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Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Immunotherapy ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Poor performance status ,Non small cell ,business ,Lung cancer ,030215 immunology - Abstract
e21601 Background: The introduction of Immunotherapy (IO) has dramatically improved the prognosis of patients (pts) with advanced Non-Small Cell Lung Cancer (NSCLC). However, data regarding the role of IO in ECOG Performance Status (PS) 2 pts are generally limited in randomized trials, and real-world evidences could support clinical decision-making. Methods: We retrospectively analyzed data about pts with stage IV NSCLC treated with IO between April 2013 and December 2019 in two Italian Centers. The aim of our study was to assess the impact of PS status (0-1 vs 2) on disease control rate (DCR), progression-free survival (PFS) and overall survival (OS). Response was classified according to RECIST v1.1 criteria. PFS and OS were estimated by the Kaplan-Meier method. Chi-square test was used to compare clinical-pathological variables: gender, age ( < 70, 70-79, ≥80 years-old), smoking status, histology (squamous, non-squamous), PDL1 expression ( < 1%, ≥1%), IO line (1°, ≥2°), number (N) of metastatic sites (1, ≥2), presence of liver and/or brain metastasis. Their impact on survival was evaluated through Cox proportional hazard models. Results: Four-hundred-one pts (35.7% female) with median age of 65.4 years (range 27-88) were studied. Baseline PS was 0 in 134 pts (33.4%), 1 in 209 pts (52.1%) and 2 in 58 pts (14.5%). 312 pts had non-squamous NSCLC, 89 squamous NSCLC. Clinical-pathological variables were uniformly distributed across PS groups except for a higher rate of liver metastasis in PS2 pts ( p= 0.046). Response evaluation was available for 386 pts. DCR was 49.7% in PS0-1 pts and 25.9% in PS2 pts ( p= 0.006). At a median follow-up of 29 months (mos), median PFS was 3.0 mos (95% CI 2.63-4.00) and 2.04 mos (95% CI 1.84-3.00) in pts with PS0-1 and 2 ( p< 0.0001). Median OS was 13.2 mos (95% CI 11.18-15.78) and 4.0 mos (95% CI 2.66-5.62) in pts with PS 0-1 and 2 respectively ( p< 0.0001). Univariate analysis showed significant correlation of PS2 status, negative PDL1, IO line ≥2, N of metastatic sites ≥2 and liver metastasis, for both PFS and OS. Multivariate analysis confirmed an independent association of PS ( p= 0.0013 for PFS, p< 0.0001 for OS), PDL1 ( p= 0.0002 for PFS, p= 0.02 for OS) and liver metastasis ( p= 0.017 for PFS, p= 0.02 for OS). The incidence of Grade 3/4 adverse events was 10.5% in PS 0-1 pts and 13.7% in PS 2 pts ( p= 0.41). Conclusions: Our data confirm reduced efficacy of IO in pts with poor PS, regardless of the N of prior therapy lines or PDL1 expression. Despite IO appears to be safe and tolerable its role remains uncertain in PS2 pts based on worse survival outcomes.
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- 2020
16. Induction of natural killer antibody-dependent cell cytotoxicity and of clinical activity of cetuximab plus avelumab in non-small cell lung cancer
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Maria Lucia Iacovino, Alessandro Morabito, Mario Capasso, Vincenzo Sforza, Vincenzo Famiglietti, Evaristo Maiello, Flora Cimmino, Giuseppe Viscardi, Morena Fasano, Achille Iolascon, Carminia Maria Della Corte, Giusi Barra, Francesca Sparano, Raimondo Di Liello, Vincenza Ciaramella, Fernando Paragliola, Fortunato Ciardiello, Floriana Morgillo, Fasano, M., Della Corte, C. M., Di Liello, R., Barra, G., Sparano, F., Viscardi, G., Iacovino, M. L., Paragliola, F., Famiglietti, V., Ciaramella, V., Cimmino, F., Capasso, M., Iolascon, A., Sforza, V., Morabito, A., Maiello, E., Ciardiello, F., and Morgillo, F.
- Subjects
Cancer Research ,Lung Neoplasms ,NK cells ,Antibodies, Monoclonal, Humanized ,NSCLC ,lcsh:RC254-282 ,Avelumab ,chemistry.chemical_compound ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Lactate dehydrogenase ,cetuximab ,Humans ,Medicine ,NK cell ,Progression-free survival ,Cytotoxicity ,Lung cancer ,neoplasms ,Original Research ,Antibody-dependent cell-mediated cytotoxicity ,Cetuximab ,business.industry ,Antibody-Dependent Cell Cytotoxicity ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Killer Cells, Natural ,Oncology ,chemistry ,Immunoglobulin G ,Cancer research ,avelumab ,ADCC ,business ,Ex vivo ,medicine.drug - Abstract
Background Antibody-dependent cell-mediated cytotoxicity (ADCC) may mediate antitumour activity of IgG1-isotype monoclonal antibody (mAb), suggesting as potential treatment combination of IgG1-mAbs, anti-epidermal growth factor receptor cetuximab and anti-programmed death-ligand-1 avelumab. Methods We evaluated ADCC induction in lung cancer cells by lactate dehydrogenase (LDH) release assay. Antitumour activity and safety of cetuximab plus avelumab were explored in a single-arm proof-of-concept study in pre-treated non-small cell lung cancer (NSCLC) patients (pt) (Cetuximab-AVElumab-lung, CAVE-Lung). Search for predictive biomarkers of response was done. Results Avelumab plus cetuximab induced ADCC in NSCLC cells in vitro in presence of natural killers (NK) from healthy donors (HD) or NSCLC pt, as effectors. Sixteen relapsed NSCLC pt were treated with avelumab plus cetuximab. Antitumour activity was observed in 6/16 pt, defined by progression free survival (PFS) ≥8 months, with 4 of them still on treatment at data lock time (range, 14–19 months). Of note, 3/6 responders had received as previous line anti-programmed death-1 therapy. In responders, clinical benefit was accompanied by significant increase in LDH release over baseline at the first radiological evaluation (8 weeks) (p=0.01) and by early skin toxicity; while in the 10 non-responders, that had PFS ≤5 months, LDH release tends to reduce. Baseline circulating DNA levels were higher in non-responders compared with responders and HD (p=0.026) and decrease in responders during therapy. Mutations in DNA damage responsive family genes were found in responders. Conclusion Cetuximab and avelumab activates NSCLC pt NK cells. Ex vivo evaluation of ADCC, circulating DNA levels and early skin toxicity may predict response to cetuximab plus avelumab in NSCLC. EUDRACT 2017-004195-58
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- 2020
17. A novel ImmunoScore, based on clinical and blood biomarkers, as prognostic model for immunotherapy in NSCLC
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Francesca Sparano, R. Borras Ferreres, N. Zanaletti, Floriana Morgillo, G. Alonso Casal, G. Bruixola, Fortunato Ciardiello, Federica Papaccio, Morena Fasano, Valentina Gambardella, A. Cervantes, Giuseppe Viscardi, R. Di Liello, M. AInsa Molla, C. Mlla De Corte, Maria Lucia Iacovino, and P. Martin Martorell
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Oncology ,medicine.medical_specialty ,Univariate analysis ,Chemotherapy ,Multivariate analysis ,Proportional hazards model ,business.industry ,medicine.medical_treatment ,Hematology ,Immunotherapy ,law.invention ,Log-rank test ,Randomized controlled trial ,law ,Internal medicine ,medicine ,Neutrophil to lymphocyte ratio ,business - Abstract
Background Immune checkpoint inhibitors (ICIs) in patients with pretreated advanced NSCLC (aNSCLC) showed an overall survival (OS) benefit over standard chemotherapy in phase III randomized clinical trials (RCTs). Nevertheless, a significant portion of patients do not benefit from ICIs. The identification of biomarkers to select patients most likely to respond to ICIs is greatly needed in clinical practice. The role of baseline clinical and blood biomarkers as prognostic of response to ICIs was investigated in patients with aNSCLC and a prognostic ImmunoScore is defined. Methods We retrospectively reviewed clinical data of aNSCLC patients consecutively treated with single agents anti PD-1 or anti PD-L1 as 2nd (81.8%) or ≥ 3rd (18.2%) line at University Hospitals of Valencia and Naples. ECOG PS, sites of metastases, neutrophil to lymphocyte ratio (NLR), LDH and albumin levels were recorded at baseline. The impact of these variables on PFS and OS was assessed through survival analyses (Kaplan Meier method), univariate (log rank test) and multivariate analyses (Cox proportional hazard model). Results The analysis included 132 pts. Median PFS and OS were 3 (95% CI 2.74-3.26) and 9 (95% CI 5.90-12.02) months respectively. The univariate analysis for PFS showed that baseline NLR>4 (p = 0.001), albumin 1 (HR 2.96, p 400 U/l (p = 0.089). The multivariate analysis for PFS confirmed as statistically significant independent negative prognostic factors PS > 1 (p = 0.001) and liver metastases (p = 0.011). Finally, according to PS, liver metastases, NLR and albumin three different prognostic groups at high (3-4 RF), intermediate (1-2 RF) and low (0 RF) risk for OS were defined. Median OS was respectively 5 (95% CI 3.45-6.55), 7 (95% CI 4.98-9.02) and 23 (95% CI 16.53-29.47) months (p Conclusion Baseline evaluation of clinical and blood biochemical parameters can be a tool to predict outcome in patients treated with ICIs for aNSCLC. Moreover, combining them in ImmunoScore may help to identify pts candidates to second or subsequent Iines of therapy who most likely will benefit from ICIs. Legal entity responsible for the study The authors. Funding Has not received any funding. Disclosure All authors have declared no conflicts of interest.
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- 2019
18. Genomic profiling of non-oncogene-addicted aNSCLC using liquid biopsy. A single institution Italian experience
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Floriana Morgillo, Francesca Sparano, C.M. Della Corte, Fernando Paragliola, R. Di Liello, Fortunato Ciardiello, Maria Lucia Iacovino, A. Di Liello, Giuseppe Viscardi, and Vincenzo Famiglietti
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medicine.medical_specialty ,Genomic profiling ,business.industry ,Hematology ,medicine.disease_cause ,Clinical trial ,Regimen ,Oncology ,Internal medicine ,Cohort ,medicine ,In patient ,KRAS ,Single institution ,Liquid biopsy ,business - Abstract
Background cfDNA analysis could represent an alternative to invasive biopsy in patients with NSCLC to better understand disease profile. We report a real-world experience of genomic assessment of an Italian patients’ cohort. Methods We evaluated 58 non-oncogene-addicted aNSCLC patients treated at our Institution from January 2018 to May 2019. All patients were characterized using cfDNA NGS Guardant360® platform (Guardant Health, Redwood City, CA). Association between a positive result (defined as cfDNA detected) and clinical features was assessed using logistic regression test. Using online library dataset (mycancergenome.org) and literature data, we divided patients in genomic clusters and performed a descriptive analysis. Results The median age of patients was 67 (range 38-85), 44 men and 14 women, 8.6% never smokers, 41.4% former and 50% current smokers. 14 patients (24.2%) had squamous cell carcinoma and 44 (75.8%) had non-squamous NSCLC, 50 of 58 at stage IV at the time of testing. Most patients were PS 0-1 per ECOG scale and has been treated with at least one systemic regimen before performing NGS. 47 of 58 patients (81%) has been considered positive at the analysis. No targetable genomic alterations per local authority has been found. More than 40 different mutated genes were detected, the most common alterations involved TP53 (60% of patients), KRAS (34%), PDGFRα (17%), EGFR (15% - not-TKI-sensitive), PI3KCA (15%), CDKN2A (15%). Chemotherapy (but not immunotherapy) and extra-thoracic radiotherapy before testing increased the probability to result positive at NGS of 4.5 and 2 folds respectively. Six mutational clusters have been identified. Most patients had genomic alterations in Replication Stress - DNA damage/repair, cell cycle - (72%) and Tyrosine Kinase Receptor/Growth Factor pathway (62%), less common mutations affected PI3K/AKT/mTOR and Hormone signaling (26% and 9% respectively). Conclusions Our experience demonstrated the feasibility of NGS-based NSCLC patients genomic profiling. The widespread of NGS platforms (and the subsequent reduction of costs) should encourage clinicians to use these methods with all patients to guarantee access to developing therapeutics and clinical trials. Legal entity responsible for the study The authors. Funding Has not received any funding. Disclosure F. Ciardiello: Advisory / Consultancy, Research grant / Funding (institution): Roche; Advisory / Consultancy, Research grant / Funding (institution): Amgen; Advisory / Consultancy, Research grant / Funding (institution): Merck; Research grant / Funding (institution): AstraZeneca; Research grant / Funding (institution): Ipsen; Advisory / Consultancy, Research grant / Funding (institution): Bayer; Advisory / Consultancy: BMS; Advisory / Consultancy: Sanofi; Advisory / Consultancy: Servier; Advisory / Consultancy: Celgene; Advisory / Consultancy: Lilly; Advisory / Consultancy: Pfizer. F. Morgillo: Advisory / Consultancy: MSD; Advisory / Consultancy: Lilly; Research grant / Funding (institution): AstraZeneca. All other authors have declared no conflicts of interest.
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- 2019
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