311 results on '"Maria Herrero"'
Search Results
2. P69 Methyl-prednisolone pulses and prolonged remission in systemic lupus erythematosus: a propensity score analysis of the longitudinal Lupus-Cruces-Bordeaux inception cohort
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Guillermo Ruiz-Irastorza, Christophe Richez, Pierre Duffau, Patrick Blanco, Estibaliz Lazaro, Ioana Ruiz-Arruza, Halbert Hernández-Negrín, Victor Moreno-Torres, Diana Paredes, Maria Herrero-Galvan, and Cedric Leonard
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Immunologic diseases. Allergy ,RC581-607 - Published
- 2024
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3. Using machine learning to model older adult inpatient trajectories from electronic health records data
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Maria Herrero-Zazo, Tomas Fitzgerald, Vince Taylor, Helen Street, Afzal N. Chaudhry, John R. Bradley, Ewan Birney, and Victoria L. Keevil
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Health technology ,Diagnostic technique in health technology ,Applied computing in medical science ,Machine learning ,Science - Abstract
Summary: Electronic Health Records (EHR) data can provide novel insights into inpatient trajectories. Blood tests and vital signs from de-identified patients’ hospital admission episodes (AE) were represented as multivariate time-series (MVTS) to train unsupervised Hidden Markov Models (HMM) and represent each AE day as one of 17 states. All HMM states were clinically interpreted based on their patterns of MVTS variables and relationships with clinical information. Visualization differentiated patients progressing toward stable ‘discharge-like’ states versus those remaining at risk of inpatient mortality (IM). Chi-square tests confirmed these relationships (two states associated with IM; 12 states with ≥1 diagnosis). Logistic Regression and Random Forest (RF) models trained with MVTS data rather than states had higher prediction performances of IM, but results were comparable (best RF model AUC-ROC: MVTS data = 0.85; HMM states = 0.79). ML models extracted clinically interpretable signals from hospital data. The potential of ML to develop decision-support tools for EHR systems warrants investigation.
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- 2023
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4. Los objetos elaborados con fibras vegetales del Neolítico Antiguo de Coves del Fem, Ulldemolins (Tarragona)
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Susagna Romero-Brugués, Maria Herrero-Otal, Raquel Piqué, Rafel Rosillo, Xavier Terradas, Oriol López-Bultó, Anna Berrocal-Barberà, and Antoni Palomo
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fibras vegetales ,cestería cosida ,cordelería ,neolítico antiguo ,vegetal fibres ,coiled basketry ,cordage ,early neolithic ,Auxiliary sciences of history ,Archaeology ,CC1-960 - Abstract
Los objetos manufacturados con materias orgánicas raramente se conservan excepto bajo condiciones ambientales específicas, ya sea en medios de sequedad constante, en contextos anaeróbicos sumergidos, bajo congelación o por carbonización. Por ello, las técnicas de cestería y cordelería, así como las materias primas utilizadas están escasamente documentadas en el registro arqueológico del Neolítico peninsular. Se aporta aquí nueva información acerca de las técnicas cesteras y de cordelería, así como de las materias primas utilizadas en Coves del Fem (Ulldemolins, Tarragona). Estos restos fueron recuperados en una fosa adscribible al Neolítico antiguo. La cestería estudiada se encuentra manufacturada a través de la técnica de espiral cosida, mientras que en el caso de la cordelería se trata de un único fragmento torsionado. Se discute su funcionalidad en un contexto de hábitat en cueva, así como su tecnología y materias primas usadas, contextualizándolos con otros ejemplos de cestería de espiral cosida de la península ibérica. ABSTRACT: Plant-based artefacts are considered one of the first technologies used by human populations playing an important role in the daily life of all societies. Even though, they are made of perishable material which usually disappears in archaeological contexts except for some specific environmental conditions that permit their conservation as arid, anaerobic, waterlogged atmospheres or carbonization. Some examples of the first implements produced by organic materials are baskets and cords which are documented since the very beginning of human populations. Nevertheless, these fibre-based materials have been excluded from archaeological studies. The lack of knowledge in this vegetal technology is a consequence of the difficulty of organic material preservation and the non-suitable analysis and identification methodologies. Even they represent a living material culture as history and ethnography demonstrate, vegetal fibres were probably used for producing indispensable everyday objects and artefacts in the past. The aim of this paper is to provide new information about vegetal technology as basketry and cordage techniques, as well as the raw materials used in Neolithic chronologies in the North-East of the Iberian Peninsula. The materials analyzed in this study were recovered at the site of Coves del Fem (Ulldemolins, Tarragona) which is a rock shelter preserved thanks to the fallen rock blocks. Basketry and cordage fragments came from the archaeological fieldwork of 2019 and were recovered inside a pit in levels of the final Early Neolithic. They were preserved thanks to the carbonization and dehydration they were submitted to. Both basketry and cordage examples from Coves del Fem, were deeply described considering their morphology and technology, as well as the raw materials they were made of. The methodology consisted of describing the technique used and taking measures of the different fragments aiming to obtain information about the way the basket and the cord were made. The identification of raw materials was performed by observing cross, peridermal and longitudinal sections of samples using a transmitted optical microscope. Descriptions were based on microanatomical observation by comparing with reference modern material and specialized technical literature. The results showed the basketry assemblage was produced using the coiling technique and all the fragments belong to a single object. In the case of the cordage fragment, it was made by twisting the fibres which had been previously mechanically processed. The identification of raw materials revealed at least the use of sedges for basketry and non-identified herbaceous plants for making cordage. The functionality of the objects is discussed, and they are also contextualized with other Neolithic fibre productions examples from the Iberian Peninsula and the Middle East, both technologically and the chosen raw materials. In summary, fibre-based productions from Coves del Fem, along with the assemblage from the lake dwelling site of La Draga, fill the gap of this archaeological record from the Iberian Peninsula to Europe, demonstrating the long history of the technical skills related to plant exploitation.
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- 2021
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5. Correction: Differential and longitudinal immune gene patterns associated with reprogrammed microenvironment and viral mimicry in response to neoadjuvant radiotherapy in rectal cancer
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Marco Ruella, Valentina Bertaina, Franco Locatelli, Antonio Camera, Biagio De Angelis, Francesca Del Bufalo, Concetta Quintarelli, Pietro Merli, Marika Guercio, Simona Manni, Iolanda Boffa, Matilde Sinibaldi, Stefano Di Cecca, Simona Caruso, Zeinab Abbaszadeh, Biancamaria Cembrola, Roselia Ciccone, Alberto Orfao, Lourdes Martin-Martin, Sara Gutierrez-Herrero, Maria Herrero-Garcia, Gianni Cazzaniga, Vittorio Nunes, Simona Songia, Paolo Marcatili, Frederikke I Marin, Luciana Vinti, and Mattia Algeri
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2021
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6. Strategy to prevent epitope masking in CAR.CD19+ B-cell leukemia blasts
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Marco Ruella, Valentina Bertaina, Franco Locatelli, Antonio Camera, Biagio De Angelis, Francesca Del Bufalo, Concetta Quintarelli, Pietro Merli, Marika Guercio, Simona Manni, Iolanda Boffa, Matilde Sinibaldi, Stefano Di Cecca, Simona Caruso, Zeinab Abbaszadeh, Biancamaria Cembrola, Roselia Ciccone, Alberto Orfao, Lourdes Martin-Martin, Sara Gutierrez-Herrero, Maria Herrero-Garcia, Gianni Cazzaniga, Vittorio Nunes, Simona Songia, Paolo Marcatili, Frederikke I Marin, Luciana Vinti, and Mattia Algeri
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background Methods We evaluated the impact of a high percentage of leukemia blast contamination in patient-derived starting material (SM) on CAR T-cell drug product (DP) manufacturing. In vitro as well as in vivo models were employed to identify characteristics of the construct associated with better profile of safety in case of inadvertent B-cell leukemia transduction during CAR T-cell manufacturing.Results The presence of large amounts of CD19+ cells in SM did not affect the transduction level of DPs, as well as the CAR T-cell rate of expansion at the end of standard production of 14 days. DPs were deeply characterized by flow cytometry and molecular biology for Ig-rearrangements, showing that the level of B-cell contamination in DPs did not correlate with the percentage of CD19+ cells in SM, in the studied patient cohort. Moreover, we investigated whether CAR design may affect the control of CAR+ leukemia cells. We provided evidences that CAR.CD19 short linker (SL) prevents complete epitope masking in CD19+CAR+ leukemia cells and we demonstrated in vitro and in vivo that CD19 +CAR(SL)+leukemic cells are killed by CAR.CD19 T-cells.Conclusions Taken together, these data suggest that a VL-VH SL may result in a safe CAR-T product, even when manufacturing starts from biological materials characterized by heavy contamination of leukemia blasts.
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- 2021
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7. Anticholinergic burden in older adult inpatients: patterns from admission to discharge and associations with hospital outcomes
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Maria Herrero-Zazo, Rachel Berry, Emma Bines, Debi Bhattacharya, Phyo K. Myint, and Victoria L. Keevil
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Anticholinergic medications are associated with adverse outcomes in older adults and should be prescribed cautiously. We describe the Anticholinergic Risk Scale (ARS) scores of older inpatients and associations with outcomes. Methods: We included all emergency, first admissions of adults ⩾65 years old admitted to one hospital over 4 years. Demographics, discharge specialty, dementia/history of cognitive concern, illness acuity and medications were retrieved from electronic records. ARS scores were calculated as the sum of anticholinergic potential for each medication (0 = limited/none; 1 = moderate; 2 = strong and 3 = very strong). We categorised patients based on admission ARS score [ARS = 0 (reference); ARS = 1; ARS = 2; ARS ⩾ 3] and change in ARS score from admission to discharge [admission and discharge ARS = 0 (reference); same; decreased; increased]. We described anticholinergic prescribing patterns by discharge specialty and explored multivariable associations between ARS score categories and mortality using logistic regression [odds ratios (ORs), 95% confidence intervals (CIs)]. Results: From 33,360 patients, 10,183 (31%) were prescribed an anticholinergic medication on admission. Mean admission ARS scores were: Cardiology and Stroke = 0.56; General Medicine = 0.78; Geriatric Medicine = 0.83; Other medicine = 0.81; Trauma and Orthopaedics = 0.66; Other Surgery = 0.65. Mean ARS did not increase from admission to discharge in any specialty but reductions varied significantly, from 4.6% (Other Surgery) to 27.7% (Geriatric Medicine) ( p
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- 2021
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8. A Minimal Information Model for Potential Drug-Drug Interactions
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Harry Hochheiser, Xia Jing, Elizabeth A. Garcia, Serkan Ayvaz, Ratnesh Sahay, Michel Dumontier, Juan M. Banda, Oya Beyan, Mathias Brochhausen, Evan Draper, Sam Habiel, Oktie Hassanzadeh, Maria Herrero-Zazo, Brian Hocum, John Horn, Brian LeBaron, Daniel C. Malone, Øystein Nytrø, Thomas Reese, Katrina Romagnoli, Jodi Schneider, Louisa (Yu) Zhang, and Richard D. Boyce
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drug-drug interaction ,adverse drug events ,minimal information model ,clinical informatics ,knowledge representation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Despite the significant health impacts of adverse events associated with drug-drug interactions, no standard models exist for managing and sharing evidence describing potential interactions between medications. Minimal information models have been used in other communities to establish community consensus around simple models capable of communicating useful information. This paper reports on a new minimal information model for describing potential drug-drug interactions. A task force of the Semantic Web in Health Care and Life Sciences Community Group of the World-Wide Web consortium engaged informaticians and drug-drug interaction experts in in-depth examination of recent literature and specific potential interactions. A consensus set of information items was identified, along with example descriptions of selected potential drug-drug interactions (PDDIs). User profiles and use cases were developed to demonstrate the applicability of the model. Ten core information items were identified: drugs involved, clinical consequences, seriousness, operational classification statement, recommended action, mechanism of interaction, contextual information/modifying factors, evidence about a suspected drug-drug interaction, frequency of exposure, and frequency of harm to exposed persons. Eight best practice recommendations suggest how PDDI knowledge artifact creators can best use the 10 information items when synthesizing drug interaction evidence into artifacts intended to aid clinicians. This model has been included in a proposed implementation guide developed by the HL7 Clinical Decision Support Workgroup and in PDDIs published in the CDS Connect repository. The complete description of the model can be found at https://w3id.org/hclscg/pddi.
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- 2021
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9. Ovary Signals for Pollen Tube Guidance in Chalazogamous Mangifera indica L.
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Jorge Lora, Veronica Perez, Maria Herrero, and Jose I. Hormaza
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Mangifera indica ,chalazogamy ,pollen tube guidance ,GABA ,γ-aminobutyric acid ,ovary signals ,Plant culture ,SB1-1110 - Abstract
Most flowering plants show porogamy in which the pollen tubes reach the egg apparatus through the micropyle. However, several species show chalazogamy, an unusual pollen tube growth, in which the pollen tubes reach the embryo sac through the chalaza. While ovary signals for pollen tube growth and guidance have been extensively studied in porogamous species, few studies have addressed the process in chalazogamous species such as mango (Mangifera indica L.), one of the five most important fruit crops worldwide in terms of production. In this study, we characterize pollen–pistil interaction in mango, paying special attention to three key players known to be involved in the directional pollen tube growth of porogamous species such as starch, arabinogalactan proteins (AGPs), and γ-aminobutyric acid (GABA). Starch grains were observed in the style and in the ponticulus at anthesis, but their number decreased 1 day after anthesis. AGPs, revealed by JIM8 and JIM13 antibodies, were homogenously observed in the style and ovary, but were more conspicuous in the nucellus around the egg apparatus. GABA, revealed by anti-GABA antibodies, was specifically observed in the transmitting tissue, including the ponticulus. Moreover, GABA was shown to stimulate in vitro mango pollen tube elongation. The results support the heterotrophic growth of mango pollen tubes in the style at the expense of starch, similarly to the observations in porogamous species. However, unlike porogamous species, the micropyle of mango does not show high levels of GABA and starch, although they were observed in the ponticulus and could play a role in supporting the unusual pollen tube growth in chalazogamous species.
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- 2021
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10. Mid-Holocene Palaeoenvironment, Plant Resources and Human Interaction in Northeast Iberia: An Archaeobotanical Approach
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Raquel Piqué, Marta Alcolea, Ferran Antolín, Marian Berihuete-Azorín, Anna Berrocal, David Rodríguez-Antón, Maria Herrero-Otal, Oriol López-Bultó, Laura Obea, and Jordi Revelles
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mid-Holocene ,archaeobotany ,Iberia ,Neolithic ,palaeoenvironment ,plant resources ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The role of the adoption of farming economies in the transformation of mid-Holocene landscapes in Northeast Iberia is under discussion given that the Neolithization coincides with the cold climatic phase dated ca. 7500–7000 cal BP. The main aim of this paper is to assess whether human activities or climate were the main driver of vegetation changes during the Middle Holocene through the study of the archaeobotanical data from three case studies: Cova del Sardo, La Draga, and Coves del Fem. The application of diverse archaeobotanical techniques to the different plant remains provides a complete picture of the vegetation composition and plant uses. During the early Neolithic, settlement surroundings were intensively exploited for firewood, wood raw material, timber, and plant fibers. The resources were obtained mainly from deciduous and pine forests, depending on the site localization, but also from riparian zones. The diversity of plants exploited was high, not only trees but shrubs and herbs. Evidence of deforestation has been identified in the settlement surroundings in La Draga and Cova del Sardo. The combination of plant exploitation with other agropastoral activities favored the expansion of colonizing species and enhanced biodiversity at a local scale.
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- 2021
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11. Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele Identification, and Incompatibility Group Assignment
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Sara Herrera, Jorge Lora, José I. Hormaza, Maria Herrero, and Javier Rodrigo
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Prunus armeniaca ,self-incompatibility ,S-alleles ,S-genotype ,ovary ,pollen tube ,Plant culture ,SB1-1110 - Abstract
Apricot (Prunus armeniaca L.) is a species of the Rosaceae that was originated in Central Asia, from where it entered Europe through Armenia. The release of an increasing number of new cultivars from different breeding programs is resulting in an important renewal of plant material worldwide. Although most traditional apricot cultivars in Europe are self-compatible, the use of self-incompatible cultivars as parental genotypes for breeding purposes is leading to the introduction of a number of new cultivars that behave as self-incompatible. As a consequence, there is an increasing need to interplant those new cultivars with cross-compatible cultivars to ensure fruit set in commercial orchards. However, the pollination requirements of many of these new cultivars are unknown. In this work, we analyze the pollination requirements of a group of 92 apricot cultivars, including traditional and newly-released cultivars from different breeding programs and countries. Self-compatibility was established by the observation of pollen tube behavior in self-pollinated flowers under the microscope. Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-(in)compatibility of 68 cultivars and the S-RNase genotype of 74 cultivars are reported herein for the first time. Approximately half of the cultivars (47) behaved as self-compatible and the other 45 as self-incompatible. Identification of S-alleles in self-incompatible cultivars allowed allocating them in 11 incompatibility groups, six of them reported here for the first time. The determination of pollination requirements and the incompatibility relationships between cultivars is highly valuable for the appropriate selection of apricot cultivars in commercial orchards and of parental genotypes in breeding programs. The approach described can be transferred to other woody perennial crops with similar problems.
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- 2018
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12. Metabolic Acidosis Is Associated With an Accelerated Decline of Allograft Function in Pediatric Kidney Transplantation
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Ariceta, Gema, Awan, Atif, Bakkaloğlu, Sevcan, Bonthuis, Marjolein, Robroeks, Charlotte Bootsma, Bouts, Antonia, Christian, Martin, Cornelissen, Marlies, Duzova, Ali, Esfandiar, Nasrin, Ghio, Luciana, Grenda, Ryszard, Guzzo, Isabella, Goni, Maria Herrero, Hogan, Julien, Hongsawong, Nattaphorn, Kanzelmeyer, Nele, Bayazit, Aysun Karabay, Aksoy, Gülşah Kaya, Knops, Noel, Kamphuis, Linda Koster, Erez, Daniella Levy, Lopez-Baez, Victor, Madrid, Alvaro, Marks, Stephen, Melk, Anette, Murer, Luisa, Pape, Lars, Peruzzi, Licia, Petrosyan, Edita, Preka, Evgenia, Printza, Nikoleta, Rachisan, Andreea Liana, Raes, Ann, Shenoy, Mohan, Soylemezoglu, Oguz, Strologo, Luca Dello, Teixeira, Ana, Topaloglu, Rezan, Weitz, Markus, Zieg, Jakub, Zlatanova, Galia, Patry, Christian, Harambat, Jerome, Ağbaş, Ayşe, Askiti, Varvara, Avramescu, Marina, Bacchetta, Justine, Bakkaloglu, Sevcan, Bontuis, Marjolein, Booth, Caroline, Dehoux, Laurene, Dizazzo, Giacomo, Drozdz, Dorota, Dursun, Ismail, Gessner, Michaela, Groothoff, Jaap, Guido, Giuliana, Klaus, Guenter, Koster-Kamphuis, Linda, Lalayiannis, Alexander, Leifheit-Nestler, Maren, Manish, Sinha, Matteucci, Chiara, Oh, Jun, Ozkaya, Ozan, Pietrement, Christine, Prytula, Agnieszka, Reusz, George, Schaefer, Franz, Schmitt, Claus Peter, Schön, Anne, Sever, Fatma Lale, Stabouli, Stella, Döven, Serra Sürmeli, Tondel, Camilla, Verrina, Enrico, Vidal, Enrico, Wallace, Dean, Arslan, Zainab, Bald, M., Fehrenbach, H., Haffner, D., Hansen, M., Hempel, C., John, U., Klaus, G., König, J., Lange-Sperandio, B., Müller, D., Oh, J., Pape, L., Pohl, M., Sauerstein, K., Schalk, G., Staude, H., Strotmann, P., Weber, L.T., Weitz, M., Berta, L., Heindl-Rusai, K., Shroff, Rukshana, van Gremberghe, Ineke, Krupka, Kai, Benetti, Elisa, Büyükkaragöz, Bahar, Kranz, Birgitta, Nalçacıoğlu, Hülya, Sellier-Leclerc, Anne-Laure, and Tönshoff, Burkhard
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- 2024
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13. Fishing Nets and String at the Final Mesolithic and Early Neolithic Site of Zamostje 2, Sergiev Posad (Russia)
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Marian Berihuete-Azorín, Olga Lozovskaya, Maria Herrero-Otal, and Raquel Piqué i Huerta
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Archeology ,Conservation ,Education - Abstract
Altres ajuts: Funding information: O.L. was funded by the Program of FSR SAS no FMZF-2022-0012. M.B.-A. is currently funded by a MSCA-IF-2019 (GA No. 893735) and during part of this research was an Alexander von Humboldt post-doc fellow. R.P. was funded by ICREA Academia Program. The site Zamostje 2, located in Sergiev-Posad district of Moscow (Russia) on the west bank of the Dubna River, has provided two Mesolithic and one Early Neolithic occupations dated from 7000 to 5400 cal BC. Thanks to the waterlogged environment, the site has an exceptional preservation. The site has yielded fishing screens, fishing fences, wooden fishing traps, and several small cordage remains elaborated with plant fibres, pine bark floats, fragments of paddles, and other wooden objects. In this work, we present the study of the fragments of cordage and fishing nets with the objective of providing new insights into the production and use of implements made of plant fibres. We have characterized the production process by analysing the morphological and technical characteristics by carrying out experimentation with plant fibres in order to obtain reference material to recognize them at an archaeological level. The analysis of 82 knots and 23 fragments of strings has allowed to determine that they were elaborated with single threads from 0.5 to 1.5 mm thick, which is noticeably smaller than most examples from other sites. All of them were elaborated with woody bast fibres.
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- 2023
14. Double Higgs boson production at TeV e+e− colliders with effective field theories: Sensitivity to BSM Higgs couplings
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Daniel Domenech Moya, Maria Ramos, Maria Herrero, and Roberto A. Morales Valbuena
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- 2022
15. One-loop corrections for WW to HH in Higgs EFT with the electroweak chiral Lagrangian
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Maria Herrero and Roberto A. Morales Valbuena
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- 2022
16. Sex, rurality and socioeconomical status in Spanish centennial population (2017)
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Louise Pilote, Alexandra Kautzky-Willer, Maria Herrero, Pedro Simón Cayuela Fuentes, Lorena Cuenca-Bermejo, Sandra Amador, Colleen M. Norris, Valeria Raparelli, Ana Maria Lucas-Ochoa, Karolina Kublickiene, and Emiliano Fernández-Villalba
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Adult ,Male ,Rural Population ,Aging ,Adolescent ,Urban Population ,Gross Domestic Product ,centenarians ,GDP per capita ,longevity ,rurality ,sex ,media_common.quotation_subject ,Population ,Gross domestic product ,NO ,Young Adult ,Life Expectancy ,Rurality ,Centennial ,Per capita ,Humans ,Child ,education ,Socioeconomic status ,Aged ,media_common ,Aged, 80 and over ,education.field_of_study ,Descriptive statistics ,Longevity ,Infant ,Cell Biology ,Middle Aged ,Geography ,Social Class ,Spain ,Child, Preschool ,Female ,Research Paper ,Demography - Abstract
World's population is exponentially aging as people reaching 100 years old has increased. The number of areas with the highest centennial population rates (Blue Zones), are significantly higher. Are there any determinant factors that favor this situation in Spain? The goal of this study was to determine the possible influence of sex, rurality and socioeconomic factors (Gross Domestic Product (GDP)) on the prevalence of the centennial population of the Spanish society. The Spanish register of inhabitants was published in 2017 by the National Statistics Institute. The analysis was carried out both by Autonomous Communities and by provinces in phases: a first descriptive analysis, followed by an inferential analysis, based on statistical tests (independent T- Student test, Pearson correlation and ANOVA). There were significant interactions between: i) sex and longevity (in favor of the female population); ii) female and rural housing and iii) female, GDP and urban areas. Feminization was proven in the longevity revolution, but, in general, GDP per Capita was not a significant survival factor on its own. This study was the first step of further analysis related to extreme longevity in Spain, which will include other dependent variables such as state of health and well-being as well as social factors.
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- 2021
17. Role of GDF-15, YKL-40 and MMP 9 in patients with end-stage kidney disease: focus on sex-specific associations with vascular outcomes and all-cause mortality
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Claudia Tucci, Liam J. Ward, Leah Hernandez, Giulia Tosti, Peter Stenvinkel, Colleen M. Norris, Maria Herrero, Torkel B. Brismar, Magnus Söderberg, Louise Pilote, Valeria Raparelli, Karolina Kublickiene, Agne Laucyte-Cibulskiene, Jonaz Ripsweden, Thomas Ebert, and Alexandra Kautzky-Willer
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Male ,medicine.medical_specialty ,Growth Differentiation Factor 15 ,Physiology ,medicine.medical_treatment ,Biomarkers ,Calcification ,Cardiovascular disease ,Chronic kidney disease ,End stage kidney disease ,TMAO ,Uraemia ,Context (language use) ,030204 cardiovascular system & hematology ,Systemic inflammation ,Gastroenterology ,NO ,Gender Studies ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Diabetes mellitus ,Internal medicine ,Medicine ,Humans ,QP1-981 ,Renal replacement therapy ,Chitinase-3-Like Protein 1 ,Prospective Studies ,030304 developmental biology ,0303 health sciences ,business.industry ,Research ,Arteriosclerosis ,medicine.disease ,Comorbidity ,3. Good health ,Matrix Metalloproteinase 9 ,embryonic structures ,Biomarker (medicine) ,Kidney Failure, Chronic ,Female ,medicine.symptom ,business ,Kidney disease - Abstract
Background Sex differences are underappreciated in the current understanding of cardiovascular disease (CVD) in association with chronic kidney disease (CKD). A hallmark of CKD is vascular aging that is characterised, amongst others, by; systemic inflammation, microbiota disbalance, oxidative stress, and vascular calcification—features linked to atherosclerosis/arteriosclerosis development. Thus, it is the necessary to introduce novel biomarkers related to athero-/arteriosclerotic damage for better assessment of vascular ageing in patients CKD. However, little is known about the relationship between uraemia and novel CVD biomarkers, such as growth differentiation factor-15 (GDF-15), cartilage glycoprotein-39 (YKL-40) and matrix metalloproteinase-9 (MMP-9). Therefore, we hypothesise that there are sex-specific relationships between GDF-15, YKL-40, MMP-9 levels in end-stage kidney disease (ESKD) patients in relation to gut microbiota, vascular calcification, inflammation, comorbidities, and all-cause mortality. Methods ESKD patients, males (n = 151) and females (n = 79), not receiving renal replacement therapy were selected from two ongoing prospective ESKD cohorts. GDF-15, YKL-40 and MMP9 were analysed using enzyme-linked immunosorbent assay kits. Biomarker levels were analysed in the context of gut microbiota-derived trimethylamine N-oxide (TMAO), vascular calcification, inflammatory response, oxidative stress, comorbidities, and all-cause mortality. Results Increased GDF-15 correlated with higher TMAO in females only, and with higher coronary artery calcification and IL-6. In females, diabetes was associated with elevated GDF-15 and MMP-9, whilst males with diabetes only had elevated GDF-15. No associations were found between biomarkers and CVD comorbidity. Deceased males and females had higher GDF-15 concentrations (p = 0.01 and p p = 0.02). Conclusions In conclusion, in males GDF-15 and YKL-40 were related to vascular calcification, inflammation, and oxidative stress, whilst in females GDF-15 was related to TMAO. Increased levels of YKL-40 and GDF-15 in males, and only GDF-15 in females, were associated with all-cause mortality. Our findings suggest that sex-specific associations of novel CVD biomarkers have a potential to affect development of cardiovascular complications in patients with ESKD.
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- 2021
18. Diagnostic and therapeutic management of vesico-ureteral reflux in pediatric kidney transplantation-Results of an online survey on behalf of the European Society for Paediatric Nephrology
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Murer, Luisa, Knops, Noël, de Jong, Huib, Konrad, Martin, Levtchenko, Elena, Jankauskiene, Augustina, Madrid-Aris, Alvaro, Marks, Stephen D., Mattoo, Tej K., Maxted, Andrew, Jahnukainen, Timo, Hooman, Nakisa, Higueras, Walter, Goñi, Maria Herrero, Herthelius, Maria, Henne, Thomas, Grenda, Ryszard, Gessner, Michaela, Gander, Romy, Zirngibl, Matthias, Galiano, Matthias, Fila, Marc, Espinosa-Román, Laura, Esfandiar, Nasrin, Dinçel, Nida Temizkan, Kılıç, Beltinge Demircioğlu, Buder, Kathrin, Dehoux, Laurène, Cornelissen, Marlies, Clothier, Joanna, Cicek, Neslihan, Christian, Martin, Bulum, Burcu, Büscher, Anja, Melgosa-Hijosa, Marta, Luithle, Tobias, Tönshoff, Burkhard, Weitz, Marcus, Zieg, Jakub, Yüksel, Selçuk, Yıldız, Nurdan, Yap, Yok-Chin, Yalçınkaya, Fatma Fatoş, Weber, Lutz T., Mincham, Christine Marie, Alonso-Melgar, Ángel, Ariceta, Gema, Mitsioni, Andromachi, Montini, Giovanni, Awan, Atif, Morgan, Henry, Bakkaloglu, SEVCAN AZİME, Baskin, Esra, Vidal, Enrico, Bekassy, Zivile, Bhimma, Rajendra, Verrina, Enrico Eugenio, Bitzan, Martin, Aki, Fazil Tuncay, Tse, Yincent, Tschumi, Sibylle, Trnka, Peter, Bjerre, Anna Kristina, Torres, Diletta, Topaloğlu, Rezan, Bootsma-Robroeks, Charlotte M., Thumfart, Julia, Teixeira, Ana, Bouts, Antonia, Tasic, Velibor, Taşdemir, Mehmet, Stabouli, Stella, Sinha, Rajiv, Silva, Ana Cristina Simões E., Shenoy, Mohan, Seeman, Tomas, Rus, Rina, Rumyantsev, Alexander, Rubik, Jacek, Roussinov, Dimitar, Reynolds, Ben, Prytula, Agnieszka, Printza, Nikoleta, Parvex, Paloma, Pape, Lars, Özçakar, Z Birsin, Müller-Sacherer, Thomas, Pediatrics, Paediatric Nephrology, and ARD - Amsterdam Reproduction and Development
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Transplantation ,therapy ,vesico-ureteral reflux ,Pediatrics, Perinatology and Child Health ,online survey ,febrile urinary tract infection ,pediatric kidney transplantation ,ureteral implantation - Abstract
© 2022 The Authors. Pediatric Transplantation published by Wiley Periodicals LLC.Background: Vesico-ureteral reflux (VUR) is considered to be a risk factor for recurrent febrile urinary tract infections and impaired renal transplant survival. Methods: An online survey supported by the European Society for Paediatric Nephrology was designed to evaluate current management strategies of VUR in native and transplanted kidneys of recipients aged
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- 2022
19. A Causal Role for the Right Dorsolateral Prefrontal Cortex in Avoidance of Risky Choices and Making Advantageous Selections
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Ignacio Obeso, Romain Ligneul, John C. Rothwell, Maria Herrero, and Marjan Jahanshahi
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0301 basic medicine ,Forgetting ,General Neuroscience ,Decision Making ,CTBS ,Prefrontal Cortex ,Cognition ,Transcranial Magnetic Stimulation ,behavioral disciplines and activities ,Iowa gambling task ,Task (project management) ,Dorsolateral prefrontal cortex ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Neuroimaging ,Gambling ,medicine ,Humans ,Right dorsolateral prefrontal cortex ,Psychology ,psychological phenomena and processes ,030217 neurology & neurosurgery ,Cognitive psychology - Abstract
In everyday life, risky decision-making relies on multiple cognitive processes including sensitivity to reinforcers, exploration, learning, and forgetting. Neuroimaging evidence suggests that the dorsolateral prefrontal cortex (DLPFC) is involved in exploration and risky decision-making, but the nature of its computations and its causal role remain uncertain. We provide evidence for the role of the DLPFC in value-independent, directed exploration on the Iowa Gambling Task (IGT) and we describe a new computational model to account for the competition of directed exploration and exploitation in guiding decisions. Forty-two healthy human participants were included in a right DLPFC, left DLPFC or sham stimulation groups using continuous theta-burst stimulation (cTBS). Immediately after cTBS, the IGT was completed. Computational modelling was used to account for exploration and exploitation with different combinations with value-based and sensitivity to reinforcers for each group. Applying cTBS to the left and right DLPFC selectively decreased directed exploration on the IGT compared to sham stimulation. Model-based analyses further indicated that the right (but not the left) DLPFC stimulation increased sensitivity to reinforcers, leading to avoidance of risky choices and promoting advantageous choices during the task. Although these findings are based on small sample sizes per group, they nevertheless elucidate the causal role of the right DLPFC in governing the exploration-exploitation tradeoff during decision-making in uncertain and ambiguous contexts.
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- 2021
20. Plants used in basketry production during the Early Neolithic in the north-eastern Iberian Peninsula
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Susagna Romero-Brugués, Maria Herrero-Otal, and Raquel Piqué i Huerta
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010506 paleontology ,Archeology ,geography ,geography.geographical_feature_category ,060102 archaeology ,biology ,Dicotyledon ,Paleontology ,Waterlogging (archaeology) ,06 humanities and the arts ,Plant Science ,biology.organism_classification ,01 natural sciences ,Archaeology ,Arid ,Prehistory ,Peninsula ,0601 history and archaeology ,Cyperaceae ,Functional studies ,Cove ,0105 earth and related environmental sciences - Abstract
Plant-based artefacts are rarely preserved in archaeological contexts in the Iberian Peninsula. Very few sites with specific conditions such as waterlogging, aridity or carbonization have preserved organic material. The aim of this paper is to establish an identification criterion for identifying organic raw materials used for manufacturing plant-based artifacts by using optical microscopy. The coiled basketry remains found in La Draga (Banyoles, Girona) and Coves del Fem (Ulldemolins, Tarragona) have been studied using this method. The materials were exceptionally well-preserved by waterlogging in the case of La Draga, and carbonized or dehydrated in Coves del Fem. Samples of both coils and stitches from all the basket remains were analysed. The anatomy of their cross-sections was observed under a transmitted light bright-dark field (BF-DF) optical microscope and compared with modern reference collections. The results show the use of at least five species corresponding to four families: three monocotyledons (Poaceae, Cyperaceae and Typhaceae) and a dicotyledon (Malvaceae). Moreover, it has been possible to distinguish the part of the plant used. Variability in raw materials implied extensive environmental knowledge. Moreover, functional studies of wooden, faunal, lithic and malacological tools suggest a local production of baskets. This paper expands the current knowledge of raw materials used for basketry purposes during prehistory in the Mediterranean area as the examples from La Draga and the Coves del Fem are the oldest basketry remains that have been studied in depth in the Iberian Peninsula and Europe.
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- 2021
21. Heart Matters: Cardiac Dysfunction and Other Autonomic Changes in Parkinson’s Disease
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Sebastian Martin-Balbuena, Valeria C Gonçalves, Emiliano Fernández-Villalba, Maria Herrero, Carla A. Scorza, Lorena Cuenca-Bermejo, and Maria José da Silva Fernandes
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0301 basic medicine ,medicine.medical_specialty ,Parkinson's disease ,Heart Diseases ,Disease ,Cardiac dysfunction ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Intensive care medicine ,Cause of death ,business.industry ,General Neuroscience ,Neurodegeneration ,Dysautonomia ,Parkinson Disease ,medicine.disease ,030104 developmental biology ,Autonomic Nervous System Diseases ,Heart failure ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Medical literature - Abstract
It has been more than 200 years since James Parkinson made the first descriptions of the disease that bears his name. Since then, knowledge about Parkinson’s disease has been improved, and its pathophysiology, diagnosis, and treatments are well described in the scientific and medical literature. However, there is no way to prevent the disease from its progressive nature yet and only its symptoms can be minimized. It is known that the process of neurodegeneration begins before the onset of motor signs and symptoms of the disease, when diagnosis is usually made. Therefore, recognizing manifested non-motor symptoms can make an early diagnosis possible and lead to a better understanding of the disease. Autonomic dysfunctions are important non-motor manifestations of Parkinson’s disease and affect the majority of patients. Importantly, heart failure is the third leading cause of death in people suffering from Parkinson’s disease. Several evidences have shown the correlation between Parkinson’s disease and the preexistence of cardiovascular diseases. Therefore, cardiovascular monitoring and identification of its dysfunctions can have a prodromal role for Parkinson’s disease. This review presents studies of the literature that can lead to a better understanding of Parkinson’s disease with special attention to its relation to heart and cardiovascular parameters.
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- 2021
22. Role of Microgliosis and NLRP3 Inflammasome in Parkinson’s Disease Pathogenesis and Therapy
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Maria Herrero, Emiliano Fernández-Villalba, Fillipe M de Araújo, Lorena Cuenca-Bermejo, Silvia Lima Costa, and Victor Diogenes Amaral da Silva
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0301 basic medicine ,Parkinson's disease ,Pars compacta ,business.industry ,Substantia nigra ,Inflammasome ,Cell Biology ,General Medicine ,Microgliosis ,medicine.disease ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,030104 developmental biology ,0302 clinical medicine ,Dopamine ,Basal ganglia ,medicine ,business ,Neuroscience ,030217 neurology & neurosurgery ,Neuroinflammation ,medicine.drug - Abstract
Parkinson's disease (PD) is a neurodegenerative disorder marked primarily by motor symptoms such as rigidity, bradykinesia, postural instability and resting tremor associated with dopaminergic neuronal loss in the Substantia Nigra pars compacta (SNpc) and deficit of dopamine in the basal ganglia. These motor symptoms can be preceded by pre-motor symptoms whose recognition can be useful to apply different strategies to evaluate risk, early diagnosis and prevention of PD progression. Although clinical characteristics of PD are well defined, its pathogenesis is still not completely known, what makes discoveries of therapies capable of curing patients difficult to be reached. Several theories about the cause of idiopathic PD have been investigated and among them, the key role of inflammation, microglia and the inflammasome in the pathogenesis of PD has been considered. In this review, we describe the role and relation of both the inflammasome and microglial activation with the pathogenesis, symptoms, progression and the possibilities for new therapeutic strategies in PD.
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- 2021
23. Impact of the kinetics of circulating anti-CD19 CAR-T cells and their populations on the outcome of DLBCL patients
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Lourdes Martín-Martín, Sara Gutiérrez-Herrero, María Herrero-García, Alejandro Martín García-Sancho, Ana Yeguas, Ana-África Martín-López, Lucía López-Corral, Estefanía Pérez-López, Marta García-Blázquez, Fermín Sánchez-Guijo, María Belén Vidriales, Giuseppe Gaipa, INCAR consortium, EuroFlow consortium, and Alberto Orfao
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2024
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24. Cricket (Acheta domesticus) flour as meat replacer in frankfurters: Nutritional, technological, structural, and sensory characteristics
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Carlos Pasqualin Cavalheiro, Claudia Ruiz-Capillas, Ana Maria Herrero, Tatiana Pintado, Thiago da Matta Pires Cruz, and Mauricio Costa Alves da Silva
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General Chemistry ,Industrial and Manufacturing Engineering ,Food Science - Published
- 2023
25. Interaction of Intrinsic Kinetics, Catalyst Durability and Internal Mass Transfer in the Oxidation of Sugar Mixtures on Gold Nanoparticle Extrudates
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Dmitry Yu. Murzin, Markus Peurla, Kari Eränen, Adriana Freites Aguilera, Juan García Serna, Tapio Salmi, Johan Wärnå, Sebastian Franz, and Maria Herrero Manzano
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General Chemical Engineering ,fungi ,food and beverages ,Nanoparticle ,Biomass ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,7. Clean energy ,Industrial and Manufacturing Engineering ,Catalysis ,chemistry.chemical_compound ,Hydrolysis ,Monomer ,020401 chemical engineering ,chemistry ,Chemical engineering ,Mass transfer ,Molecule ,0204 chemical engineering ,0210 nano-technology ,Sugar - Abstract
Sugar monomers originating from well-controlled hydrolysis of hemicelluloses appearing in biomass are important platform molecules and they can be further valorized by catalytic hydrogenation, oxid...
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- 2020
26. Woody and non-woody forest raw material at the early Neolithic site of La Draga (Banyoles, Spain)
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Maria Herrero-Otal, Susagna Romero-Brugués, Oriol López-Bultó, Marian Berihuete-Azorín, Josep Girbal, Raquel Piqué, Xavier Terradas, Anna Franch, Jordi Revelles, Maxime Rageot, Patrick Gassmann, Antoni Palomo, Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, and European Research Council
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Geography ,Neolithic ,Forest raw material ,Raw material ,Archaeology ,La Draga - Abstract
The early Neolithic site of La Draga (Banyoles, Spain) has provided a singular sample of plant-crafts, wooden tools, and other plant remains preserved in waterlogged conditions. This exceptional context of plant remains preservation provides an incomparable frame to study past botanical knowledge and the emergence and devel- opment of plant transformation technologies. The goal of this paper is to provide an integrated synthesis of the plant resources used by the inhabitants of La Draga, paying special attention to the main taxa used, the environ- ments exploited and the plant acquisition strategies. Through the results of the analysis of plant-crafts, timber and wooden tools, charcoal remains, exudates, fungi, and microremains as pollen, we discuss types of forest and plants exploited to obtain raw materials and the human impact on vegetation. The plant raw materials identified at La Draga indicate the exploitation of local resources available in deciduous and riparian forests where an anthropic disturbance is documented contemporary with the occupation., This work was supported by Ministerio de Ciencia, Innovacion y Universidades, Spain, projects: WOOD- PDLAKE -Archaeological Wooden Pile-Dwelling in Mediterranean European lakes: strategies for their exploitation, monitoring and conservation, JPICH -Conservation, Protection and Use Joint Call (PCI2020-111992)-, PID2019-109254GB-C21 -Paleoambiente, modelización del paisaje y análisis del uso de plantas en la transición a la agricultura en el Noreste de La Península Ibérica-. The archaeological excavation at La Draga was funded by the Departament de Cultura (Generalitat de Catalunya) CLT009/18/00050. The authors are member of the research group supported by AGAUR- Generalitat de Catalunya: TEDAS 2017 SGR 243 and ASD 2017 SGR 995. RP also acknowledges the sup- port of Catalan Institution for Research and Advanced Studies (ICREA, Generalitat de Catalunya, Spain), through the ICREA Academia Program. M. Berihuete-Azorín (2018 BP 00272) received funding from the postdoctoral fellowships pro- gramme Beatriu de Pinós, funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Skfodowska-Curie grant agreement 801370.M.
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- 2022
27. Using Machine Learning to Model Older Adult Inpatient Trajectories From Electronic Health Records Data
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Maria Herrero-Zazo, Tomas Fitzgerald, Vince Taylor, Helen Street, Afzal N. Chaudhry, John R. Bradley, Ewan Birney, Victoria L. Keevil, Herrero-Zazo, Maria [0000-0001-7793-3296], Apollo - University of Cambridge Repository, Herrero-Zazo, M [0000-0001-7793-3296], and Keevil, VL [0000-0001-6148-0640]
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Diagnostic technique in health technology ,History ,Multidisciplinary ,Polymers and Plastics ,Machine learning ,Applied computing in medical science ,Health technology ,Business and International Management ,Industrial and Manufacturing Engineering - Abstract
Electronic Health Records (EHR) data can provide novel insights into inpatient trajectories. Blood tests and vital signs from de-identified patients' hospital admission episodes (AE) were represented as multivariate time-series (MVTS) to train unsupervised Hidden Markov Models (HMM) and represent each AE day as one of 17 states. All HMM states were clinically interpreted based on their patterns of MVTS variables and relationships with clinical information. Visualization differentiated patients progressing toward stable 'discharge-like' states versus those remaining at risk of inpatient mortality (IM). Chi-square tests confirmed these relationships (two states associated with IM; 12 states with ≥1 diagnosis). Logistic Regression and Random Forest (RF) models trained with MVTS data rather than states had higher prediction performances of IM, but results were comparable (best RF model AUC-ROC: MVTS data = 0.85; HMM states = 0.79). ML models extracted clinically interpretable signals from hospital data. The potential of ML to develop decision-support tools for EHR systems warrants investigation., This research was supported by the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014). VLK was funded by a MRC/NIHR Clinical Academic Research Partnership Grant (CARP; grant code: MR/T023902/1). VT is supported by Cancer Research UK. EB and TF were funded by the EMBL European Bioinformatics Institute (EMBL-EBI).
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- 2022
28. Efficacy and safety of bendamustine‐containing bridging therapy in R/R LBCL patients receiving CD19 CAR T‐cells
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Gloria Iacoboni, Mario A. Sánchez‐Salinas, Kai Rejeski, Ana Á. Martín‐López, Mi Kwon, Víctor Navarro, Katarzyna A. Jalowiec, Rafael Hernani, Juan L. Reguera‐Ortega, Laura Gallur, Viktoria Blumenberg, María Herrero‐García, Claire Roddie, Ana Benzaquén, Javier Delgado‐Serrano, Rebeca Bailén, Cecilia Carpio, Paula Amat, Lucia López‐Corral, Lourdes Martín‐Martín, Mariana Bastos, Marion Subklewe, Maeve O'Reilly, and Pere Barba
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract Bridging therapy (BT) after leukapheresis is required in most relapsed/refractory (R/R) large B‐cell lymphoma (LBCL) patients receiving chimeric antigen receptor (CAR) T cells. Bendamustine‐containing regimens are a potential BT option. We aimed to assess if this agent had a negative impact on CAR‐T outcomes when it was administered as BT. We included R/R LBCL patients from six centers who received systemic BT after leukapheresis from February 2019 to September 2022; patients who only received steroids or had pre‐apheresis bendamustine exposure were excluded. Patients were divided into two BT groups, with and without bendamustine. Separate safety and efficacy analyses were carried out for axi‐cel and tisa‐cel. Of 243 patients who received BT, bendamustine (benda) was included in 62 (26%). There was a higher rate of BT progressors in the non‐benda group (62% vs. 45%, p = 0.02). Concerning CAR‐T efficacy, complete responses were comparable for benda versus non‐benda BT cohorts with axi‐cel (70% vs. 53%, p = 0.12) and tisa‐cel (44% vs. 36%, p = 0.70). Also, 12‐month progression‐free and overall survival were not significantly different between BT groups with axi‐cel (56% vs. 43% and 71% vs. 63%) and tisa‐cel (25% vs. 26% and 52% vs. 48%); there were no differences when BT response was considered. CAR T‐cell expansion for each construct was similar between BT groups. Regarding safety, CRS G ≥3 (6% vs. 6%, p = 0.79), ICANS G ≥3 (15% vs. 17%, p = 0.68), severe infections, and neutropenia post‐infusion were comparable among BT regimens. BT with bendamustine‐containing regimens is safe for patients requiring disease control during CAR T‐cell manufacturing.
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- 2024
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29. Combined 1-Deoxynojirimycin and Ibuprofen Treatment Decreases Microglial Activation, Phagocytosis and Dopaminergic Degeneration in MPTP-Treated Mice
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F M Araújo, Vda Silva, A M Lucas-Ochoa, N.J. Menezes-Filho, Silvia Lima Costa, Cristina Estrada, Rejane Conceição Santana, Emiliano Fernández-Villalba, M. de Oliveira, Virginia Izura, Tcs Costa, and Maria Herrero
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0301 basic medicine ,Pharmacology ,Microglia ,Chemistry ,Pars compacta ,MPTP ,Immunology ,Neurodegeneration ,Dopaminergic ,Neuroscience (miscellaneous) ,Substantia nigra ,medicine.disease ,Neuroprotection ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,nervous system ,medicine ,Immunology and Allergy ,030217 neurology & neurosurgery ,Neuroinflammation - Abstract
Inflammation is a predominant aspect of neurodegenerative diseases and experimental studies performed in animal models of Parkinson's disease (PD) suggesting that a sustained neuroinflammation exacerbates the nigrostriatal degeneration pathway. The central role of microglia in neuroinflammation has been studied as a target for potential neuroprotective drugs for PD, for example nonsteroidal anti-inflammatory drugs (NSAIDs) and matrix metalloproteinases (MMP) inhibitors that regulates microglial activation and migration. The aim of this study was to investigate the neuroprotective response of the iminosugar 1-deoxynojirimycin (1-DNJ) and compare its effect with a combined treatment with ibuprofen. MPTP-treated mice were orally dosed with ibuprofen and/or 1-DNJ 1. Open-field test was used to evaluate behavioral changes. Immunohistochemistry for dopaminergic neurons marker (TH+) and microglia markers (Iba-1+; CD68+) were used to investigate neuronal integrity and microglial activation in the substantia nigra pars compacta (SNpc). The pro-inflammatory cytokines TNF-α and IL-6 were analysed by qPCR. Treatments with either 1-DNJ or Ibuprofen alone did not reduce the damage induced by MPTP intoxication. However, combined treatment with 1-DNJ and ibuprofen prevents loss of mesencephalic dopaminergic neurons, decreases the number of CD68+/ Iba-1+ cells, the microglia/neurons interactions, and the pro-inflammatory cytokines, and improves behavioral changes when compared with MPTP-treated animals. In conclusion, these data demonstrate that the combined treatment with a MMPs inhibitor (1-DNJ) plus an anti-inflammatory drug (ibuprofen) has neuroprotective effects open for future therapeutic interventions. Graphical Abstract MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) is a protoxicant that, after crossing the Blood Brain Barrier, is metabolized by astrocytic MAO-B to MPDP+, a pyridinium intermediate, which undergoes further two-electron oxidation to yield the toxic metabolite MPP+ (methyl-phenyltetrahydropyridinium) that is then selectively transported into nigral neurons via the mesencephalic dopamine transporter. In this study, we demonstrated that MPTP induced death of dopaminergic neurons, microgliosis, increase of gliapses, motor impairment and neuroinflammation in mice, which were inhibited by combined 1-deoxynojirimycin and ibuprofen treatment.
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- 2020
30. Strategy to prevent epitope masking in CAR.CD19+ B-cell leukemia blasts
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Pietro Merli, Gianni Cazzaniga, Franco Locatelli, Vittorio Nunes, Zeinab Abbaszadeh, Alberto Orfao, Matilde Sinibaldi, Mattia Algeri, Frederikke Isa Marin, Maria Herrero-Garcia, Stefano Di Cecca, Paolo Marcatili, Biagio De Angelis, Concetta Quintarelli, Sara Gutiérrez-Herrero, Luciana Vinti, Lourdes Martín-Martín, Roselia Ciccone, Biancamaria Cembrola, Simona Songia, Simona Caruso, Iolanda Boffa, Simona Manni, Valentina Bertaina, Marika Guercio, Antonio Camera, Francesca Del Bufalo, Marco Ruella, Quintarelli, C, Guercio, M, Manni, S, Boffa, I, Sinibaldi, M, DI Cecca, S, Caruso, S, Abbaszadeh, Z, Camera, A, Cembrola, B, Ciccone, R, Orfao, A, Martin-Martin, L, Gutierrez-Herrero, S, Herrero-Garcia, M, Cazzaniga, G, Nunes, V, Songia, S, Marcatili, P, Marin, F, Ruella, M, Bertaina, V, Vinti, L, Del Bufalo, F, Algeri, M, Merli, P, De Angelis, B, Locatelli, F, Cancer Research UK, Associazione Italiana per la Ricerca sul Cancro, Asociación Española Contra el Cáncer, and Ministero della Salute
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0301 basic medicine ,Cancer Research ,receptor ,receptors ,Epitope ,Epitopes ,Mice ,0302 clinical medicine ,hemic and lymphatic diseases ,Immunology and Allergy ,hematologic neoplasms ,RC254-282 ,Receptors, Chimeric Antigen ,biology ,medicine.diagnostic_test ,Chemistry ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Leukemia ,Oncology ,Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA ,030220 oncology & carcinogenesis ,translational medical research ,Molecular Medicine ,immunotherapy ,Immunology ,cell engineering ,adoptive ,CD19 ,Flow cytometry ,03 medical and health sciences ,In vivo ,Cell Line, Tumor ,Leukemia, B-Cell ,medicine ,Animals ,Humans ,Pharmacology ,Immune Cell Therapies and Immune Cell Engineering ,medicine.disease ,Chimeric antigen receptor ,In vitro ,Disease Models, Animal ,030104 developmental biology ,B-cell leukemia ,chimeric antigen ,Cancer research ,biology.protein ,human activities ,hematologic neoplasm - Abstract
[Abstract]: Chimeric antigen receptor T-cells (CAR T-cells) for the treatment of relapsing/refractory B-cell precursor acute lymphoblastic leukemia have led to exciting clinical results. However, CAR T-cell approaches revealed a potential risk of CD19-/CAR+ leukemic relapse due to inadvertent transduction of leukemia cells. [Methods]: We evaluated the impact of a high percentage of leukemia blast contamination in patient-derived starting material (SM) on CAR T-cell drug product (DP) manufacturing. In vitro as well as in vivo models were employed to identify characteristics of the construct associated with better profile of safety in case of inadvertent B-cell leukemia transduction during CAR T-cell manufacturing. [Results]: The presence of large amounts of CD19+ cells in SM did not affect the transduction level of DPs, as well as the CAR T-cell rate of expansion at the end of standard production of 14 days. DPs were deeply characterized by flow cytometry and molecular biology for Ig-rearrangements, showing that the level of B-cell contamination in DPs did not correlate with the percentage of CD19+ cells in SM, in the studied patient cohort. Moreover, we investigated whether CAR design may affect the control of CAR+ leukemia cells. We provided evidences that CAR.CD19 short linker (SL) prevents complete epitope masking in CD19+CAR+ leukemia cells and we demonstrated in vitro and in vivo that CD19 +CAR(SL)+leukemic cells are killed by CAR.CD19 T-cells. [Conclusions]: Taken together, these data suggest that a VL-VH SL may result in a safe CAR-T product, even when manufacturing starts from biological materials characterized by heavy contamination of leukemia blasts., The experimental work was supported by grants awarded by Ricerca Finalizzata GR-2013 02359212 (CQ), GR-2016-02364546 (BDA), RF-2016- 02364388 (FL), Accelerator Award-Cancer Research UK/AIRC/AECC-INCAR project (FL and AO), Associazione Italiana Ricerca per la Ricerca sul Cancro (AIRC)-Special Project 5×1000 no. 9962 (FL), AIRC IG 2018 id. 21724 (FL), Ricerca Corrente (FL, CQ, BDA), Ministero dell’Università e della Ricerca (Grant PRIN 2017 to FL); Italian Healthy Ministry project on CAR T RCR-2019-23669115 (coordinator FL), Independent Research grant AIFA (FL PI: 2016 call).
- Published
- 2021
31. A New Tool to Study Parkinsonism in the Context of Aging: MPTP Intoxication in a Natural Model of Multimorbidity
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Consuelo Sanchez-Rodrigo, María Guillén-Díaz, Elisa Pizzichini, Valeria C Gonçalves, Maria Herrero, Ana-María González-Cuello, Emiliano Fernández-Villalba, Lorena Cuenca-Bermejo, and Elena Aguilar-Moñino
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0301 basic medicine ,Male ,Parkinson's disease ,O. degus ,Nigrostriatal pathway ,Hippocampus ,Striatum ,neuroinflammation ,chemistry.chemical_compound ,0302 clinical medicine ,neurotoxicity ,Biology (General) ,Spectroscopy ,biology ,Behavior, Animal ,MPTP ,Parkinsonism ,Dopaminergic ,neurodegeneration ,General Medicine ,Computer Science Applications ,Chemistry ,medicine.anatomical_structure ,1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine ,QH301-705.5 ,Neurotoxins ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,Parkinsonian Disorders ,medicine ,biology.domesticated_animal ,Animals ,Physical and Theoretical Chemistry ,Molecular Biology ,QD1-999 ,Inflammation ,Dopaminergic Neurons ,Organic Chemistry ,aging ,MPTP Poisoning ,medicine.disease ,Octodon degus ,Octodon ,Neostriatum ,Disease Models, Animal ,030104 developmental biology ,chemistry ,nervous system ,Parkinson’s disease ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The diurnal rodent Octodon degus (O. degus) is considered an attractive natural model for Alzheimer’s disease and other human age-related features. However, it has not been explored so far if the O. degus could be used as a model to study Parkinson’s disease. To test this idea, 10 adult male O. degus were divided into control group and MPTP-intoxicated animals. Motor condition and cognition were examined. Dopaminergic degeneration was studied in the ventral mesencephalon and in the striatum. Neuroinflammation was also evaluated in the ventral mesencephalon, in the striatum and in the dorsal hippocampus. MPTP animals showed significant alterations in motor activity and in visuospatial memory. Postmortem analysis revealed a significant decrease in the number of dopaminergic neurons in the ventral mesencephalon of MPTP animals, although no differences were found in their striatal terminals. We observed a significant increase in neuroinflammatory responses in the mesencephalon, in the striatum and in the hippocampus of MPTP-intoxicated animals. Additionally, changes in the subcellular expression of the calcium-binding protein S100β were found in the astrocytes in the nigrostriatal pathway. These findings prove for the first time that O. degus are sensitive to MPTP intoxication and, therefore, is a suitable model for experimental Parkinsonism in the context of aging.
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- 2021
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32. 614 - PREDICTORES TERAPÉUTICOS DE REMISIÓN PROLONGADA EN UNA COHORTE DE PACIENTES CON LUPUS ERITEMATOSO SISTÉMICO
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Ruiz, Diana Paredes, Galván, María Herrero, Peleteiro, Adriana Soto, de Echavarri, Cristina González, Iglesias, Daniel Martín, Arruza, Ioana Ruiz, Lazaro, Estibaliz, and Irastorza, Guillermo Ruiz
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- 2023
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33. Immunohistochemical Study of 2 Cases of Coxsackie A6–Induced Atypical Hand-Foot-and-Mouth Disease
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Maria Herrero, Mar Llamas-Velasco, Heinz Kutzner, and Javier Fraga
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Male ,Pathology ,medicine.medical_specialty ,Dermatology ,Coxsackievirus ,medicine.disease_cause ,Asymptomatic ,Virus ,Pathology and Forensic Medicine ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Enterovirus ,biology ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,biology.organism_classification ,Immunohistochemistry ,Reverse transcription polymerase chain reaction ,biology.protein ,Female ,medicine.symptom ,Antibody ,Hand, Foot and Mouth Disease ,business ,Spongiosis - Abstract
An atypical clinical variant of hand-foot-and-mouth disease (HFMD) with more extensive lesions and affecting adults has emerged during the past years, usually associated to the Coxsackievirus serotype A6 (CV-A6). We present a 19-year-old woman with a 3-day evolution eruption of papulovesicular lesions, which first appeared around the mouth and frontal area and rapidly spread. In addition, we present a 61-year-old man with a 4-day evolution asymptomatic eruption of papulovesicular lesions in both the hands and feet after suffering a cold 1 week before. Skin biopsies of both patients showed intraepidermal vesicles with spongiosis and ballooning, leading to reticular degeneration, apoptotic keratinocytes, and epidermal necrosis of the upper layers with neutrophil sloughing. Immunohistochemical studies for Coxsackie, Enterovirus, herpes virus, adenovirus, and measles were all negative. Cultures of blister fluid, reverse transcription polymerase chain reaction of skin biopsies, blood tests and serologies for exanthematic virus, and serum viral arrays were also negative. Only reverse transcription polymerase chain reaction of blister fluid confirmed Cocksakie A6. In conclusion, immunohistochemical studies with the commercially available viral antibodies do not seem to be useful in atypical HFMD cases. In these cases, to determine the typical histopathological features in HE is the fastest diagnostic aid.
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- 2019
34. Arabinogalactan proteins mediate intercellular crosstalk in the ovule of apple flowers
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Maria Herrero, Juan M. Losada, Ministerio de Ciencia e Innovación (España), Gobierno de Aragón, Losada Rodríguez, Juan Manuel [0000-0002-7966-5018], and Losada Rodríguez, Juan Manuel
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0106 biological sciences ,Pollination ,Arabinogalactan proteins ,Flowers ,Pollen Tube ,Plant Science ,Biology ,01 natural sciences ,Mucoproteins ,Human fertilization ,Pollen tube ,Arabinogalactan ,Ovule ,Plant Proteins ,Malus × domestica ,Gametophyte ,Reproduction ,Embryo sac ,Apple ,food and beverages ,Embryo ,Cell Biology ,Filiform apparatus ,Cell biology ,Malus ,Seeds ,Pollen ,010606 plant biology & botany - Abstract
41 Pags.- 11 Figs. The definitive version is available at: https://link.springer.com/journal/497., Glycoproteins are significant players in the dialog that takes place between growing pollen tubes and the stigma and style in the angiosperms. Yet, information is scarce on their possible involvement in the ovule, a sporophytic organ that hosts the female gametophyte. Apple flowers have a prolonged lapse of time between pollination and fertilization, offering a great system to study the developmental basis of glycoprotein secretion and their putative role during the last stages of the progamic phase and early seed initiation. For this purpose, the sequential pollen tube elongation within the ovary was examined in relation to changes in arabinogalactan proteins (AGPs) in the tissues of the ovule before and after fertilization. To evaluate what of these changes are developmentally regulated, unpollinated and pollinated flowers were compared. AGPs paved the pollen tube pathway in the ovules along the micropylar canal, and the nucellus entrance toward the synergids, which also developmentally accumulated AGPs at the filiform apparatus. Glycoproteins vanished from all these tissues following pollen tube passage, strongly suggesting a role in pollen–ovule interaction. In addition, AGPs marked the primary cell walls of the haploid cells of the female gametophyte, and they further built up in the cell walls of the embryo sac and developing embryo, layering the interactive walls of the three generations hosted in the ovule, the maternal sporophytic tissues, the female gametophyte, and the developing embryo., This work was supported by Ministerio de Ciencia e Innovación (MICINN)-FEDER [AGL2006-13529-C02-01, AGL 12621-C02-01, AGL 2012–40239] and Gobierno de Aragón [group A43]. JIM antibodies distribution was partly supported by NSF grants [DBI-0421683, RCN 009281]. JML was supported by a FPI fellowship [BES-2007-16059] from MICINN.
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- 2019
35. Different factors involved in the low fruit set of mango (Mangifera indica)
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Maria Herrero, Verónica Pérez, José I. Hormaza, Ministerio de Economía y Competitividad (España), European Commission, Gobierno de Aragón, Cabildo Insular de La Palma, Hormaza Urroz, José Ignacio [0000-0001-5449-7444], and Hormaza Urroz, José Ignacio
- Subjects
Gynoecium ,Pollination ,fungi ,reproductive biology ,food and beverages ,Horticulture ,Biology ,medicine.disease_cause ,pollen tube ,pollen germination ,Inflorescence ,Hermaphrodite ,Pollen ,medicine ,Mangifera ,Pollen tube ,Cultivar ,paternity ,ovule - Abstract
5 Pags. The definitive version is available at: https://www.ishs.org/ishs-book/1231, Mango (Mangifera indica) is a tropical fruit tree originated in southeastern Asia, which is cultivated in regions with tropical and subtropical climates worldwide. Mango cultivation has significantly increased in the last decades, often spreading to areas where environmental conditions are not the most favorable for optimal fruit set. In this work, we describe the reproductive biology of mango in the coastal areas of southern Spain, the furthest region from the equator where mango is produced commercially at a significant scale. As in other regions, mango trees produced several inflorescences per tree with hundreds to thousands of male and hermaphrodite flowers, depending on the cultivar, but only a very small percentage of the flowers are able to set fruits due to a massive drop of flowers and fruitlets. Although about half of the flowers in the inflorescence are male flowers and, consequently, contribute to this massive drop, a high proportion of the hermaphrodite flowers were not receptive to pollen germination. In receptive flowers, the characterization of the pollen tube pathway within the pistil showed chalazogamy. A detailed study of the ovules indicates that a high proportion of them showed degenerated embryo sacs, both before and after fertilization. Paternity analysis with molecular markers showed a high proportion of embryos resulting from self-pollination in orchards planted with a single cultivar. However, without pollination restrictions, the offspring shows a higher proportion of genotypes resulting from cross-pollination, suggesting preferential cross-fertilization. The variable response of this parameter, together to the variable proportion of hermaphrodite/male flower ratio, also obtained in response to environmental conditions, suggests a reproductive plasticity in this species., This research was supported by the Ministerio de Economía y Competitividad – European Regional Development Fund, European Union (AGL2012-40239 and AGL2013-43732-R) and Gob. Aragón A-43. V. Pérez was supported by a Cabildo de La Palma-CSIC PhD scholarship.
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- 2019
36. Approach to plant craft techniques from the mat impressions on the bases of Early Bronze Age ceramic vessels: The case of Cova Fonda (Spain)
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Susagna Romero-Brugués, Evdoxia Tzerpou, Maria Herrero-Otal, Anna Homs, Oriol López Bultó, Igor Bodganovic, Javier Fanlo, Antoni Palomo, and Raquel Piqué
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Archeology - Published
- 2022
37. Cardiac Tyrosine Hydroxylase Activation and Mb-Comt in Dyskinetic Monkeys
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Lorena Cuenca-Bermejo, Victor Bautista-Hernandez, Pilar Almela, Pablo Gallo-Soljancic, María-Luisa Laorden, Emiliano Fernández-Villalba, José Enrique Yuste, Vicente de Pablos, and Maria Herrero
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Male ,Nervous system ,Cardiac function curve ,medicine.medical_specialty ,Tyrosine 3-Monooxygenase ,Science ,Dopamine ,animal diseases ,Population ,Cardiology ,Nigrostriatal pathway ,Catechol O-Methyltransferase ,Article ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Phosphorylation ,education ,education.field_of_study ,Multidisciplinary ,Tyrosine hydroxylase ,business.industry ,Dopaminergic Neurons ,MPTP ,Dopaminergic ,Membrane Proteins ,Parkinson Disease ,Immunohistochemistry ,Corpus Striatum ,nervous system diseases ,Enzyme Activation ,Macaca fascicularis ,Endocrinology ,medicine.anatomical_structure ,chemistry ,nervous system ,Ventricle ,cardiovascular system ,Medicine ,business ,Biomarkers ,Neuroscience - Abstract
The impact of age-associated disorders is increasing as the life expectancy of the population increments. Cardiovascular diseases and neurodegenerative disorders, such as Parkinson’s disease, have the highest social and economic burden and increasing evidence show interrelations between them. Particularly, dysfunction of the cardiovascular nervous system is part of the dysautonomic symptoms of Parkinson’s disease, although more studies are needed to elucidate the role of cardiac function on it. We analyzed the dopaminergic system in the nigrostriatal pathway of Parkinsonian and dyskinetic monkeys and the expression of some key proteins in the metabolism and synthesis of catecholamines in the heart: total and phosphorylated (phospho) tyrosine hydroxylase (TH), and membrane (MB) and soluble (S) isoforms of catechol-O-methyl transferase (COMT). The dopaminergic system was significantly depleted in all MPTP-intoxicated monkeys. MPTP- and MPTP+L-DOPA-treated animals also showed a decrease in total TH expression in both right (RV) and left ventricle (LV). We found a significant increase of phospho-TH in both groups (MPTP and MPTP+L-DOPA) in the LV, while this increase was only observed in MPTP-treated monkeys in the RV. MB-COMT analysis showed a very significant increase of this isoform in the LV of MPTP- and MPTP+L-DOPA-treated animals, with no significant differences in S-COMT levels. These data suggest that MB-COMT is the main isoform implicated in the cardiac noradrenergic changes observed after MPTP treatment, suggesting an increase in NA metabolism. Moreover, the increase of TH activity indicates that cardiac noradrenergic neurons still respond despite MPTP treatment.
- Published
- 2021
38. Mid-Holocene Palaeoenvironment, Plant Resources and Human Interaction in Northeast Iberia: An Archaeobotanical Approach
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Anna Berrocal, Oriol López-Bultó, L. Obea, Marta Alcolea, Marian Berihuete-Azorín, Ferran Antolín, Maria Herrero-Otal, Raquel Piqué, Jordi Revelles, and David Rodríguez-Antón
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Technology ,010506 paleontology ,QH301-705.5 ,QC1-999 ,Palaeoenvironment ,Biodiversity ,mid-Holocene ,Firewood ,01 natural sciences ,Plant resources ,plant resources ,Paleoethnobotany ,Deforestation ,Mid-Holocene ,0601 history and archaeology ,General Materials Science ,Biology (General) ,Neolithic ,QD1-999 ,Instrumentation ,Holocene ,0105 earth and related environmental sciences ,Riparian zone ,Fluid Flow and Transfer Processes ,geography ,geography.geographical_feature_category ,060102 archaeology ,palaeoenvironment ,Ecology ,Physics ,Process Chemistry and Technology ,General Engineering ,06 humanities and the arts ,Vegetation ,15. Life on land ,Engineering (General). Civil engineering (General) ,Computer Science Applications ,Chemistry ,Deciduous ,Archaeobotany ,Iberia ,TA1-2040 ,archaeobotany - Abstract
Altres ajuts: Institució Catalana de Recerca i Estudis Avançats FJC2018 The role of the adoption of farming economies in the transformation of mid-Holocene landscapes in Northeast Iberia is under discussion given that the Neolithization coincides with the cold climatic phase dated ca. 7500-7000 cal BP. The main aim of this paper is to assess whether human activities or climate were the main driver of vegetation changes during the Middle Holocene through the study of the archaeobotanical data from three case studies: Cova del Sardo, La Draga, and Coves del Fem. The application of diverse archaeobotanical techniques to the different plant remains provides a complete picture of the vegetation composition and plant uses. During the early Neolithic, settlement surroundings were intensively exploited for firewood, wood raw material, timber, and plant fibers. The resources were obtained mainly from deciduous and pine forests, de-pending on the site localization, but also from riparian zones. The diversity of plants exploited was high, not only trees but shrubs and herbs. Evidence of deforestation has been identified in the settlement surroundings in La Draga and Cova del Sardo. The combination of plant exploitation with other agropastoral activities favored the expansion of colonizing species and enhanced biodiversity at a local scale.
- Published
- 2021
39. Methods for prospectively incorporating gender into health sciences research
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Valeria Raparelli, Alexandra Kautzy-Willer, Karolina Kublickiene, Colleen M. Norris, Christina P. Tadiri, Maria Herrero, L. Pilote, and Michal Abrahamowicz
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Gender Equity ,Male ,medicine.medical_specialty ,Epidemiology ,Socio-culturale ,Sex and gender-based analysis ,Developmental psychology ,03 medical and health sciences ,0302 clinical medicine ,Sex Factors ,5. Gender equality ,medicine ,Humans ,Relevance (information retrieval) ,Statistical analysis ,030212 general & internal medicine ,Sex Distribution ,10. No inequality ,gender-related variables ,health equity ,population health ,sex and gender-based analysis ,Selection (genetic algorithm) ,Health equity ,Quality of Health Care ,Biological variable ,Population health ,Gender-related variables ,3. Good health ,Epidemiologic Studies ,Research Design ,Female ,Public Health ,Psychology ,Construct (philosophy) ,Psychosocial ,030217 neurology & neurosurgery ,Needs Assessment ,Biomedical sciences - Abstract
Numerous studies have demonstrated that sex (a biological variable) and gender (a psychosocial construct) impact health and have discussed the mechanisms that may explain these relationships. Funding agencies have called for all health researchers to incorporate sex and gender into their studies; however, the way forward has been unclear to many, particularly due to the varied definition of gender. We argue that just as there is no standardized definition of gender, there can be no standardized measurement thereof. However, numerous measurable gender-related variables may influence individual or population-level health through various pathways. The initial question should guide the selection of specific gender-related variables based on their relevance to the study, to prospectively incorporate gender into research. We outline various methods to provide clarification on how to incorporate gender into the design of prospective clinical and epidemiological studies as well as methods for statistical analysis.
- Published
- 2021
40. Determinants of perceived health and unmet healthcare needs in universal healthcare systems with high gender equality
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Louise Pilote, Karolina Kublickiene, Teresa Gisinger, Alexandra Kautzky-Willer, Maria Herrero, Colleen M. Norris, Christina P. Tadiri, and Valeria Raparelli
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Gender Equity ,Male ,medicine.medical_specialty ,Canada ,media_common.quotation_subject ,Health Status ,Immigration ,Socio-culturale ,Country/cultural determinants of health ,Health Services Accessibility ,03 medical and health sciences ,Social determinants of health ,0302 clinical medicine ,5. Gender equality ,Epidemiology ,Health care ,medicine ,Humans ,030212 general & internal medicine ,10. No inequality ,media_common ,Health Services Needs and Demand ,Public health ,030505 public health ,Patient-reported outcomes ,business.industry ,Public Health, Environmental and Occupational Health ,3. Good health ,Socioeconomic Factors ,Scale (social sciences) ,Austria ,Marital status ,Universal Health Care ,Female ,Biostatistics ,Public aspects of medicine ,RA1-1270 ,0305 other medical science ,business ,Delivery of Health Care ,Demography ,Research Article - Abstract
Background Patient attitudes about health and healthcare have emerged as important outcomes to assess in clinical studies. Gender is increasingly recognized as an intersectional social construct that may influence health. Our objective was to determine potential sex differences in self-reported overall health and access to healthcare and whether those differences are influenced by individual social factors in two relatively similar countries. Methods Two public health surveys from countries with high gender equality (measured by UN GII) and universal healthcare systems, Canada (CCHS2014, n = 57,041) and Austria (AT-HIS2014, n = 15,212), were analysed. Perceived health was assessed on a scale of 1 (very bad) to 4 (very good) and perceived unmet healthcare needs was reported as a dichotomous variable (yes/no). Interactions between sex and social determinants (i.e. employment, education level, immigration and marital status) on outcomes were analysed. Results Individuals in both countries reported high perceived health (Scoring > 2, 85.0% in Canada, 79.9% in Austria) and a low percentage reported unmet healthcare needs (4.6% in Canada, 10.7% in Austria). In both countries, sex and several social factors were associated with high perceived health, and a sex-by-marital status interaction was observed, with a greater negative impact of divorce for men. Female sex was positively associated with unmet care needs in both countries, and sex-by-social factors interactions were only detected in Canada. Conclusions The intersection of sex and social factors in influencing patient-relevant outcomes varies even among countries with similar healthcare and high gender equality.
- Published
- 2021
41. Ploidia levels in pyrenic and apyrenic 'pitangueira' accessions
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Kelli Pirola, Marcelo Dotto, Américo Wagner Júnior, Maria Herrero, Ana Maria Castillo, Castillo Alonso, Ana María, and Castillo Alonso, Ana María [0000-0002-6143-3227]
- Subjects
Myrtaceae ,Pharmaceutical Science ,poliploidia ,seedless fruit ,Biology ,Eugenia uniflora ,Polyploidy ,Fluorescence intensity ,Horticulture ,Complementary and alternative medicine ,Meiosis ,Uv laser ,Pharmacology (medical) ,fruto sem semente ,Ploidy - Abstract
4 Pags.- 1 Fig. Esta obra está licenciada com uma Licença Creative Commons Atribuição-NãoComercial-CompartilhaIgual 4.0 Internacional., [EN] Pitangueira (E. uniflora) is considered a diploid species with n = 11 and 2n = 22 chromosomes, in genotypes with the presence of seeds in the fruits. With the pitangueira production of apyrenic fruits existence, such behavior may be related to the ploidy level. The objective of this study was to determine the ploidy level of the pitangueira accessions producing fruit with and without seed, as well as to observe the meiotic behavior and possible chromosomal abnormalities. To check the ploidy level of the pitangueira, freshly expanded mature leaves were collected from the pyrenic pitangueira and from two pyrenic accessions, with suspensions of intact nuclei being prepared. Samples were analyzed in a flow cytometer equipped with multiple parameters data acquisition and UV laser. All analyses were performed using peak-height detection (>6000 fluorescent events, for example, nuclei, were analyzed per sample) and logarithmic amplification. The data were presented as histograms of the number of nuclei along the y-axis and the relative fluorescence intensity on the x-axis. The pyrenean pitangueira and other two pyrenic accessions were characterized as diploid., [PO] A pitangueira (E. uniflora) é considerada espécie diplóide com n=11 e 2n=22 cromossomos, em genótipos com presença de sementes nos frutos. Com a existência de pitangueira produtora de frutos apirênicos, tal comportamento pode estar relacionado ao nível de ploidia. O objetivo deste estudo foi determinar o nível de ploidia dos acessos de pitangueira produtora de frutos sem e com semente, bem como, observar o comportamento meiótico e possíveis anormalidades cromossômicas. Para verificar o nível de ploidia da pitangueira, folhas maduras recém-expandidas foram coletadas da pitangueira apirênica e de dois acessos pirênicos, sendo preparados suspensões de núcleos intactos. As amostras foram analisadas em citômetro de fluxo equipado com aquisição de dados de múltiplos parâmetros e de laser UV. Todas as análises foram realizadas utilizando detecção de pico-altura (>6000 eventos fluorescentes, por exemplo, núcleos, foram analisados por amostra) e amplificação logarítmica. Os dados foram apresentados como histogramas do número de núcleos ao longo do eixo-y e a intensidade da fluorescência relativa no eixo-x. A pitangueira apirênica e outros dois acessos pirênicos, foram caracterizadas como diplóides.
- Published
- 2021
42. Sex, Gender, and Cardiovascular Health in Canadian and Austrian Populations
- Author
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Karolina Kublickiene, Louise Pilote, Zahra Azizi, Teresa Gisinger, Valeria Raparelli, Carola Deischinger, Alexandra Kautzky-Willer, Khaled El Emam, Maria Herrero, Uri Bender, and Colleen M. Norris
- Subjects
Male ,Canada ,Heart disease ,Cardiovascular health ,Health Status ,Socio-culturale ,Overweight ,Interquartile range ,Diabetes mellitus ,Surveys and Questionnaires ,Vegetables ,Diabetes Mellitus ,Medicine ,Humans ,Sex Distribution ,LS4_7 ,business.industry ,Smoking ,Middle Aged ,medicine.disease ,Obesity ,Confidence interval ,Diet ,Cross-Sectional Studies ,Cardiovascular Diseases ,Austria ,Fruit ,Community health ,Hypertension ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Demography - Abstract
Background Evidence differentiating the effect of biological sex from psychosociocultural factors (gender) in different societies and its relation to cardiovascular diseases is scarce. We explored the association between sex, gender, and cardiovascular health (CVH) among Canadian (CAN) and Austrian (AT) populations. Methods The Canadian Community Health Survey (CCHS) (n = 63,522; 55% female) and Austrian Health Interview Survey (AT-HIS) (n = 15,771; 56% female) were analyzed in a cross-sectional survey design. The CANHEART/ATHEART index, a measure of ideal CVH composed of 6 cardiometabolic risk factors (smoking, physical activity, fruit and vegetable consumption, overweight/obesity, diabetes, and hypertension; range 0-6; higher scores reflecting better CVH) was calculated for both databases. A composite measure of psychosociocultural gender was computed for each country (range 0-1, higher score identifying characteristics traditionally ascribed to women). Results Median CANHEART 4 (interquartile range 3-5) and CAN gender scores 0.55 (0.49-0.60) were similar to median ATHEART 4 (3-5) and AT gender scores 0.55 (0.46-0.64). Although higher gender scores (CCHS: β = −1.33, 95% confidence interval [CI] −1.44 to −1.22; AT-HIS: β = −1.08, 95% CI −1.26 to −0.89)) were associated with worse CVH, female sex (CCHS: β = 0.35, 95% CI (0.33-0.37); AT-HIS: β = 0.60, 95% CI (0.55-0.64)) was associated with better CVH in both populations. In addition, higher gender scores were associated with increased prevalence of heart disease compared with female sex. The magnitude of this risk was higher in Austrians. Conclusions These results demonstrate that individuals with characteristics typically ascribed to women reported poorer cardiovascular health and higher risk of heart disease, independently from biological sex and baseline CV risk factors, in both countries. Female sex exhibited better CV health and a lower prevalence of heart disease than male in both populations. However, gender factors and magnitude of gender impact varied by country.
- Published
- 2021
43. Ovary Signals for Pollen Tube Guidance in Chalazogamous
- Author
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Jorge, Lora, Veronica, Perez, Maria, Herrero, and Jose I, Hormaza
- Subjects
ovary signals ,chalazogamy ,GABA ,food and beverages ,Mangifera indica ,ponticulus ,Plant Science ,pollen tube guidance ,Original Research ,γ-aminobutyric acid - Abstract
Most flowering plants show porogamy in which the pollen tubes reach the egg apparatus through the micropyle. However, several species show chalazogamy, an unusual pollen tube growth, in which the pollen tubes reach the embryo sac through the chalaza. While ovary signals for pollen tube growth and guidance have been extensively studied in porogamous species, few studies have addressed the process in chalazogamous species such as mango (Mangifera indica L.), one of the five most important fruit crops worldwide in terms of production. In this study, we characterize pollen–pistil interaction in mango, paying special attention to three key players known to be involved in the directional pollen tube growth of porogamous species such as starch, arabinogalactan proteins (AGPs), and γ-aminobutyric acid (GABA). Starch grains were observed in the style and in the ponticulus at anthesis, but their number decreased 1 day after anthesis. AGPs, revealed by JIM8 and JIM13 antibodies, were homogenously observed in the style and ovary, but were more conspicuous in the nucellus around the egg apparatus. GABA, revealed by anti-GABA antibodies, was specifically observed in the transmitting tissue, including the ponticulus. Moreover, GABA was shown to stimulate in vitro mango pollen tube elongation. The results support the heterotrophic growth of mango pollen tubes in the style at the expense of starch, similarly to the observations in porogamous species. However, unlike porogamous species, the micropyle of mango does not show high levels of GABA and starch, although they were observed in the ponticulus and could play a role in supporting the unusual pollen tube growth in chalazogamous species.
- Published
- 2020
44. Identification of differentially expressed genes profiles in a combined mouse model of Parkinsonism and colitis
- Author
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Emiliano Fernández-Villalba, A. L. Gil-Martinez, A. Parrado, Lorena Cuenca-Bermejo, Consuelo Sanchez-Rodrigo, Ana González-Cuello, Maria Herrero, S. Vyas, Universidad de Murcia, Expression des Gènes et comportement adaptatifs = Gene regulation and adaptative behaviors (NPS), Neuroscience Paris Seine (NPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Research work of the authors was supported by the Spanish Ministry of Science and Innovation (FIS PI13 01293), Fundación Séneca (19540/PI/14) and 'Prediction of cognitive properties of new drug candidates for neurodegenerative diseases in early clinical development' (European Community’s Seventh Framework Programme (FP7/2007-2013) for the Innovative Medicine Initiative under Grant Agreement No. 115009) to MTH., European Project: 115009,EC:FP7:SP1-JTI,IMI-JU-01-2008,PHARMA-COG(2010), Bodescot, Myriam, Prediction of cognitive properties of new drug candidates for neurodegenerative diseases in the early clinical development - PHARMA-COG - - EC:FP7:SP1-JTI2010-01-01 - 2015-12-31 - 115009 - VALID, Gene Regulation and Adaptive Behaviours Team [Paris], Neurosciences Paris Seine (NPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), and Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Male ,0301 basic medicine ,Parkinson's disease ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,lcsh:Medicine ,Cellular homeostasis ,Inflammation ,Biology ,Systemic inflammation ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Article ,Transcriptome ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Colitis ,lcsh:Science ,Multidisciplinary ,Parkinsonism ,MPTP ,lcsh:R ,Dextran Sulfate ,Neurodegeneration ,[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,MPTP Poisoning ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,medicine.disease ,Cellular neuroscience ,[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,3. Good health ,Disease Models, Animal ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Cancer research ,lcsh:Q ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Different cellular mechanisms have been described as being potentially involved in the progression of neurodegeneration in Parkinson’s disease, although their role is still unclear. The present study aimed to identify in detail, through differentially expressed genes analysis by bioinformatics approaches, the molecular mechanisms triggered after a systemic insult in parkinsonian mice. To address this objective, we combined a dextran sodium sulfate (DSS)-induced ulcerative colitis experimental mice model with an acute 1-methyl-4-phenyl-1,2,3,6-tetradropyridine (MPTP) intoxication. The animals were divided into four experimental groups based on the different treatments: (i) control, (ii) DSS, (iii) MPTP and (iv) MPTP + DSS. The data obtained by microarray and functional enrichment analysis point out the implication of different molecular mechanisms depending on the experimental condition. We see, in the striatum of animals intoxicated only with DSS, dysfunction processes related to the blood. On the other hand, oxidative stress processes are more prominent at the MPTP intoxicated mice. Finally, differentially expressed genes within the MPTP + DSS show functional enrichment in inflammation and programmed cell death. Interestingly, we identify a significant synergistic negative effect of both toxins since the expression of differentially expressed genes (DEGs) related to balanced cellular homeostasis was not enough to prevent processes associated with cell death. This work provides detailed insights into the involvement of systemic inflammation, triggered after an insult in the colon, in the progression of the degeneration in Parkinsonism. In this way, we will be able to identify promising therapeutic targets that prevent the contribution of inflammatory processes in the progression of Parkinson’s disease.
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- 2020
45. Octodon degus: a natural model of multimorbidity for ageing research
- Author
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Emiliano Fernández-Villalba, Elisa Pizzichini, Maria Herrero, Maria Egle De Stefano, Ana González-Cuello, and Lorena Cuenca-Bermejo
- Subjects
0301 basic medicine ,Aging ,biomedicine ,Biology ,Biochemistry ,neuroscience ,Type ii diabetes ,03 medical and health sciences ,0302 clinical medicine ,Genetic similarity ,Alzheimer Disease ,biology.domesticated_animal ,Multimorbidity ,Animals ,Octodon degus, ageing, biomedicine, multimorbidity, neuroscience ,Cognitive decline ,Molecular Biology ,Biomedicine ,business.industry ,Octodon degus ,Octodon ,Disease Models, Animal ,030104 developmental biology ,Neurology ,Diabetes Mellitus, Type 2 ,ageing ,Ageing ,business ,Neuroscience ,030217 neurology & neurosurgery ,Biotechnology - Abstract
Integrating the multifactorial processes co-occurring in both physiological and pathological human conditions still remains one of the main challenges in translational investigation. Moreover, the impact of age-associated disorders has increased, which underlines the urgent need to find a feasible model that could help in the development of successful therapies. In this sense, the Octodon degus has been indicated as a 'natural' model in many biomedical areas, especially in ageing. This rodent shows complex social interactions and high sensitiveness to early-stressful events, which have been used to investigate neurodevelopmental processes. Interestingly, a high genetic similarity with some key proteins implicated in human diseases, such as apolipoprotein-E, β-amyloid or insulin, has been demonstrated. On the other hand, the fact that this animal is diurnal has provided important contribution in the field of circadian biology. Concerning age-related diseases, this rodent could be a good model of multimorbidity since it naturally develops cognitive decline, neurodegenerative histopathological hallmarks, visual degeneration, type II diabetes, endocrinological and metabolic dysfunctions, neoplasias and kidneys alterations. In this review we have collected and summarized the studies performed on the Octodon degus through the years that support its use as a model for biomedical research, with a special focus on ageing.
- Published
- 2020
46. Bidirectional gut-to-brain and brain-to-gut propagation of synucleinopathy in non-human primates
- Author
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Alice Prigent, Niels Kruse, Miquel Vila, Cristina Estrada, Marie-Laure Thiolat, Nishant N. Vaikath, Nuria García-Carrillo, Gregory Porras, Maria Herrero, Omar M. A. El-Agnaf, Marie-Laure Arotcarena, Philippe Aubert, Erwan Bezard, Maddalena Tasselli, Jose A. Obeso, Sandrine Camus, Mathieu Bourdenx, Pascal Derkinderen, Ines Trigo Damas, Benjamin Dehay, Sandra Dovero, Brit Mollenhauer, Interdisciplinary Institute for Neuroscience (IINS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut des Maladies Neurodégénératives [Bordeaux] (IMN), The Enteric Nervous System in gut and brain disorders [U1235] (TENS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), University Medical Center Göttingen (UMG), Centro de Investigacion Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Instituto de Salud Carlos III [Madrid] (ISC), University of San Pablo, Universidad de Murcia, Instituto de Investigaciones Biomédicas Alberto Sols [Madrid, Spain] (IIBM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Universidad Autonoma de Madrid (UAM), Hamad Bin Khalifa University (HBKU), Vall d’Hebron Research Institute (VHIR), Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Universidad Autónoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), and Dehay, Benjamin
- Subjects
Male ,0301 basic medicine ,Parkinson's disease ,Neuroimmunomodulation ,[SDV]Life Sciences [q-bio] ,Enteric Nervous System ,03 medical and health sciences ,0302 clinical medicine ,α-synuclein ,biology.animal ,medicine ,Animals ,Humans ,Aged ,Synucleinopathies ,Lewy body ,biology ,business.industry ,Neurodegeneration ,neurodegeneration ,Brain ,Parkinson Disease ,Vagus Nerve ,medicine.disease ,3. Good health ,Vagus nerve ,nervous system diseases ,[SDV] Life Sciences [q-bio] ,030104 developmental biology ,Dorsal motor nucleus ,nervous system ,alpha-Synuclein ,Parkinson’s disease ,gut ,Female ,Lewy Bodies ,Enteric nervous system ,Neurology (clinical) ,monkey ,business ,Neuroscience ,030217 neurology & neurosurgery ,Papio ,Baboon - Abstract
In Parkinson’s disease, synucleinopathy is hypothesized to spread from the enteric nervous system, via the vagus nerve, to the CNS. Here, we compare, in baboon monkeys, the pathological consequences of either intrastriatal or enteric injection of α-synuclein-containing Lewy body extracts from patients with Parkinson’s disease. This study shows that patient-derived α-synuclein aggregates are able to induce nigrostriatal lesions and enteric nervous system pathology after either enteric or striatal injection in a non-human primate model. This finding suggests that the progression of α-synuclein pathology might be either caudo-rostral or rostro-caudal, varying between patients and disease subtypes. In addition, we report that α-synuclein pathological lesions were not found in the vagal nerve in our experimental setting. This study does not support the hypothesis of a transmission of α-synuclein pathology through the vagus nerve and the dorsal motor nucleus of the vagus. Instead, our results suggest a possible systemic mechanism in which the general circulation would act as a route for long-distance bidirectional transmission of endogenous α-synuclein between the enteric and the central nervous systems. Taken together, our study provides invaluable primate data exploring the role of the gut-brain axis in the initiation and propagation of Parkinson’s disease pathology and should open the door to the development and testing of new therapeutic approaches aimed at interfering with the development of sporadic Parkinson’s disease.
- Published
- 2020
47. Imported cysticercosis in Spain: A retrospective case series from the +REDIVI Collaborative Network
- Author
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Herrador, Zaida, Perez-Molina, Jose A., Henriquez Camacho, Cesar Augusto, Rodriguez-Guardado, Azucena, Bosch-Nicolau, Pau, Calabuig, Eva, Dominguez-Castellano, Angel, Asuncion Perez-Jacoiste, Maria, Ladron de Guevara, M. Concepcion, Mena, Ana, Manuel Ruiz-Giardin, Jose, Torrus, Diego, Wikman-Jorgensen, Philip, Benito, Agustin, Lopez-Velez, Rogelio, Aguilera, Paloma, Martinez Serrano, Maria, Garcia Rodriguez, Magdalena, Diaz Menendez, Marta, Meije, Yolanda, Martinez-Montauti, Joaquim, Sanz, Xavier, Pacheco Tenza, Isabel, Gonzalez Cuello, Inmaculada, Martinez Lopez, Belen, Llenas-Garcia, Jara, Masia, Mar, Padilla, Sergio, Romero, Monica, Ramos Rincon, Jose Manuel, Suarez, Ines, Perez-Ayala, Ana, Maria Herrero, Juan, Lizasoain, Manuel, Rojo, Pablo, Matarranz, Mariano, Zarco, Carlos, Fernandez Suarez, Jonathan, Boga Ribeiro, Jose Antonio, Goikoetxea Aguirre, Josune, Zubero Sulibarria, Miren Zurine, Sanmartin Lopez, Juan Victor, Velasco Arribas, Maria, Penaranda Vera, Maria, Molina, Israel, Sanchez Montalva, Adrian, Salvador, Fernando, Monge-Maillo, Begona, Norman, Francesca, Chamorro Tojeiro, Sandra, Trevino-Maruri, Begoria, Serre Delcor, Nuria, Soriano-Arandes, Antonio, Pou Ciruelo, Diana, Bocanegra, Cristina, REDIVI Study Grp, Instituto de Salud Carlos III, European Regional Development Fund, Instituto de Salud Carlos III - ISCIII, and European Regional Development Fund (ERDF/FEDER)
- Subjects
Adult ,Male ,medicine.medical_specialty ,Pediatrics ,030231 tropical medicine ,Neurocysticercosis ,Disease ,Albendazole ,Serology ,03 medical and health sciences ,Epilepsy ,0302 clinical medicine ,parasitic diseases ,Taenia solium ,medicine ,Travel medicine ,Humans ,030212 general & internal medicine ,Retrospective Studies ,business.industry ,Cysticercosis ,Public Health, Environmental and Occupational Health ,Neglected Diseases ,medicine.disease ,Europe ,medicine.drug_formulation_ingredient ,Neglected diseases ,Infectious Diseases ,Spain ,business ,Imported infectious diseases ,Travel Medicine ,medicine.drug - Abstract
Background: Neurocysticercosis (NCC) is the most common parasitic neurological disease worldwide and a major cause of epilepsy. Spain is the country reporting the highest number of NCC imported cases in Europe. Methodology: Retrospective case series of NCC patients registered in the +REDIVI Network from October 1, 2009 to July 2018. A specific questionnaire, including clinical and diagnostic characteristics, was created and sent to the collaborator centers. Results: 46 cases were included in the analysis. 55% were male, mean age of 40 years. 95.6% were migrants. The median duration since migration from an endemic area was 10 years. Predominant nationalities were Ecuadorians (50%) and Bolivians (30.4%). Frequent locations were parenchymal (87%), subarachnoid (26.1%) and intraventricular cysts (10.9%). Serological analysis was performed in 91.3%, being 54.8% positive. Most prevalent clinical manifestations were persistent headache (60.9%), epilepsy (43.5%) and visual changes (13%). Patients were mainly treated with albendazole (76.1%), corticosteroids (67.4%), and anticonvulsionants (52.2%). 82.5% had a favorable clinical outcome. Conclusions: Most NCC cases were long-standing migrants. Few clinical differences were observed depending on the cysticerci location. The treatment was often not according to current recommendations, and no uniform criteria were followed when it came to the therapeutic regimen. NCC case management in Spain (including clinician awareness and laboratory capacity improvements) needs to be strengthened., We would thanks Maria Jesus Perteguer from the National Center of Microbiology for the information and update on NCC lab techniques currently performed in Spain. The corresponding author's affiliation centre belongs to the ISCIII-Sub. Gral. Redes-Network Biomedical Research on Tropical Diseases (RICET in Spanish) grant RD16CIII/0003/0001, RD16/0027/0020, RD16CIII/0003/0001 and the European Regional Development Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Published
- 2020
48. Identification of distinct pathological signatures induced by patient-derived α-synuclein structures in non-human primates
- Author
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S. Bohic, I. Trigo Damas, Omar M. A. El-Agnaf, Nuria García-Carrillo, Erwan Bezard, Ariadna Recasens, Jose A. Obeso, Niels Kruse, Evelyne Doudnikoff, Philippe Aubert, T. Leste-Lasserre, Alice Prigent, C. Perier, Nishant N. Vaikath, Salvatore Novello, Marie Laure Thiolat, Sandra Dovero, Miquel Vila, Florent Laferrière, C. Sandt, Michele Morari, Mathieu Bourdenx, Brit Mollenhauer, Aurélien Nioche, Cristina Estrada, Michel Goillandeau, S. Camus, M. L. Arotcarena, Gregory Porras, Maria Herrero, Nicolas Rougier, Pascal Derkinderen, Benjamin Dehay, Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Mnemonic Synergy (Mnemosyne), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), European Synchrotron Radiation Facility (ESRF), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), University Medical Center Göttingen (UMG), Università degli Studi di Ferrara (UniFE), Institut National de la Santé et de la Recherche Médicale (INSERM), Instituto de Salud Carlos III [Madrid] (ISC), Universidad de Murcia, Hamad Bin Khalifa University (HBKU), Rougier, Nicolas P., Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, and Università degli Studi di Ferrara = University of Ferrara (UniFE)
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Primates ,Amyloid ,Programmed cell death ,animal diseases ,LS5_11 ,Disease ,Biology ,Protein aggregation ,NO ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Mediator ,medicine ,Animals ,Humans ,Research Articles ,030304 developmental biology ,0303 health sciences ,[SCCO.NEUR]Cognitive science/Neuroscience ,[SCCO.NEUR] Cognitive science/Neuroscience ,Dopaminergic ,Neurodegeneration ,food and beverages ,SciAdv r-articles ,Parkinson Disease ,medicine.disease ,nervous system diseases ,machine learning ,nervous system ,Cellular Neuroscience ,parkinson's disease ,alpha-synuclein ,non-human primates ,spreading ,alpha-Synuclein ,Lewy Bodies ,Neuroscience ,030217 neurology & neurosurgery ,Intracellular ,Research Article - Abstract
Machine learning–based approach unravels distinct pathological signatures induced by patient-derived α-synuclein seeds in monkeys., Dopaminergic neuronal cell death, associated with intracellular α-synuclein (α-syn)–rich protein aggregates [termed “Lewy bodies” (LBs)], is a well-established characteristic of Parkinson’s disease (PD). Much evidence, accumulated from multiple experimental models, has suggested that α-syn plays a role in PD pathogenesis, not only as a trigger of pathology but also as a mediator of disease progression through pathological spreading. Here, we have used a machine learning–based approach to identify unique signatures of neurodegeneration in monkeys induced by distinct α-syn pathogenic structures derived from patients with PD. Unexpectedly, our results show that, in nonhuman primates, a small amount of singular α-syn aggregates is as toxic as larger amyloid fibrils present in the LBs, thus reinforcing the need for preclinical research in this species. Furthermore, our results provide evidence supporting the true multifactorial nature of PD, as multiple causes can induce a similar outcome regarding dopaminergic neurodegeneration.
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- 2019
49. Electrical stimulation or MK-801 in the inferior colliculus improve motor deficits in MPTP-treated mice
- Author
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Cristina Estrada, Ana González-Cuello, Liana Melo-Thomas, A. L. Gil-Martinez, Maria Herrero, Rainer K.W. Schwarting, and Lorena Cuenca
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Male ,0301 basic medicine ,Inferior colliculus ,N-Methylaspartate ,Deep brain stimulation ,Microinjections ,Deep Brain Stimulation ,medicine.medical_treatment ,Motor Disorders ,Stimulation ,Motor Activity ,Toxicology ,Open field ,Midbrain ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Glutamatergic ,0302 clinical medicine ,medicine ,Animals ,business.industry ,General Neuroscience ,MPTP ,MPTP Poisoning ,Inferior Colliculi ,030104 developmental biology ,nervous system ,chemistry ,Rotarod Performance Test ,NMDA receptor ,Dizocilpine Maleate ,business ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The inferior colliculus (IC) is an important midbrain relay station for the integration of descending and ascending auditory information. Additionally, the IC has been implicated in processing sensorimotor responses. Glutamatergic and GABAergic manipulations in the IC can improve motor deficits as demonstrated by the animal model of haloperidol-induced catalepsy. However, how the IC influences motor function remains unclear. We investigated the effects of either intracollicular deep brain stimulation (DBS) or microinjection of the glutamatergic antagonist MK-801 or the agonist NMDA in C57BL/6J mice chronically treated with saline or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). After DBS or microinjections, the mice were submitted to rotarod and open field tests, respectively. DBS in the IC was effective to increase the time spent on the rotarod in MPTP-treated mice. After unilateral microinjection of MK-801, but not NMDA, MPTP-treated mice increased the distance travelled in the open field (p 0.05). In conclusion, intracollicular DBS or MK-801 microinjection can improve motor performance in parkinsonian mice suggesting the IC as a new and non-conventional therapeutic target in motor impairment.
- Published
- 2018
50. Activity and thermal stability of antioxidants by differential scanning calorimetry and electron spin resonance spectroscopy
- Author
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Giuffrida, Francesca, Destaillats, Frédéric, Egart, Maria Herrero, Hug, Bernadette, Golay, Pierre-Alain, Skibsted, Leif H., and Dionisi, Fabiola
- Published
- 2007
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