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Identification of differentially expressed genes profiles in a combined mouse model of Parkinsonism and colitis
- Source :
- Scientific Reports, Scientific Reports, 2020, 10 (1), pp.13147. ⟨10.1038/s41598-020-69695-4⟩, Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.13147. ⟨10.1038/s41598-020-69695-4⟩, Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Different cellular mechanisms have been described as being potentially involved in the progression of neurodegeneration in Parkinson’s disease, although their role is still unclear. The present study aimed to identify in detail, through differentially expressed genes analysis by bioinformatics approaches, the molecular mechanisms triggered after a systemic insult in parkinsonian mice. To address this objective, we combined a dextran sodium sulfate (DSS)-induced ulcerative colitis experimental mice model with an acute 1-methyl-4-phenyl-1,2,3,6-tetradropyridine (MPTP) intoxication. The animals were divided into four experimental groups based on the different treatments: (i) control, (ii) DSS, (iii) MPTP and (iv) MPTP + DSS. The data obtained by microarray and functional enrichment analysis point out the implication of different molecular mechanisms depending on the experimental condition. We see, in the striatum of animals intoxicated only with DSS, dysfunction processes related to the blood. On the other hand, oxidative stress processes are more prominent at the MPTP intoxicated mice. Finally, differentially expressed genes within the MPTP + DSS show functional enrichment in inflammation and programmed cell death. Interestingly, we identify a significant synergistic negative effect of both toxins since the expression of differentially expressed genes (DEGs) related to balanced cellular homeostasis was not enough to prevent processes associated with cell death. This work provides detailed insights into the involvement of systemic inflammation, triggered after an insult in the colon, in the progression of the degeneration in Parkinsonism. In this way, we will be able to identify promising therapeutic targets that prevent the contribution of inflammatory processes in the progression of Parkinson’s disease.
- Subjects :
- Male
0301 basic medicine
Parkinson's disease
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
lcsh:Medicine
Cellular homeostasis
Inflammation
Biology
Systemic inflammation
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Article
Transcriptome
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Colitis
lcsh:Science
Multidisciplinary
Parkinsonism
MPTP
lcsh:R
Dextran Sulfate
Neurodegeneration
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MPTP Poisoning
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
medicine.disease
Cellular neuroscience
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
3. Good health
Disease Models, Animal
030104 developmental biology
Gene Expression Regulation
chemistry
Cancer research
lcsh:Q
medicine.symptom
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, 2020, 10 (1), pp.13147. ⟨10.1038/s41598-020-69695-4⟩, Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.13147. ⟨10.1038/s41598-020-69695-4⟩, Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
- Accession number :
- edsair.doi.dedup.....526cdd500297c3d03b79c487cd766de5