116 results on '"María Cabrerizo"'
Search Results
2. Datos analíticos como predicción de la duración del ingreso hospitalario en infecciones por parechovirus humanos
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Silvia Gómez-Anca, Paula Fresno Jorge, María Cabrerizo, María de Ceano-Vivas La Calle, and Cristina Calvo
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Pediatrics ,RJ1-570 - Published
- 2024
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3. Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
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Fabiana Gámbaro, Ana Belén Pérez, Eduardo Agüera, Matthieu Prot, Luis Martínez-Martínez, María Cabrerizo, Etienne Simon-Loriere, and Maria Dolores Fernandez-Garcia
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Medicine ,Science - Abstract
Abstract New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs.
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- 2021
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4. Neurodevelopmental Outcomes of Infants Younger Than 90 Days Old Following Enterovirus and Parechovirus Infections of the Central Nervous System
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María de Ceano-Vivas, M. Luz García, Ana Velázquez, Fernando Martín del Valle, Ana Menasalvas, Amaia Cilla, Cristina Epalza, M. Pilar Romero, María Cabrerizo, and Cristina Calvo
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enterovirus ,parechovirus ,central nervous system infection ,young infant ,developmental outcome ,ASQ-3 test ,Pediatrics ,RJ1-570 - Abstract
Enteroviruses (EVs) and human parechoviruses (HPeVs) are a major cause of central nervous system (CNS) infection in young infants. They have been implicated in neurodevelopmental delay, however limited data are available. The aim of this study is to describe the clinical outcome of young infants and to assess and compare the medium-term neurodevelopment following CNS infections caused by EV and HPeV. A multicentre observational ambispective study was conducted between May 2013 and March 2018. Children under 3 months of age with EV or HPeV CNS infection excluding encephalitis were included. Infants were contacted 1 year after the acute infection and their neurological development was evaluated using the Ages and Stages Questionnaire-3 (ASQ-3). If any area assessed was abnormal during the first round of tests, a second round was completed 6 to 12 months later. Forty-eight young infants with EV and HPeV CNS infection were identified: 33 (68.8%) were positive for EV and 15 (31.3%) for HPeV. At first assessment 14 out of 29 EV (48.3%) and 3 out of 15 HPeV (20%) positive cases presented some developmental concern in the ASQ-3 test. EV-positive infants showed mild and moderate alteration in all domains analyzed and HPeV-positive infants showed mild alterations only in gross and fine motor domains. Significant alterations in communication were observed in EV-positive but not in HPeV-positive infants (31 vs. 0%, p = 0.016). At second assessment 4 out of 13 EV-positive patients (30.8%) showed mild to moderate concerns in communication and gross motor function domains and 3 out of 13 (23.1%) showed significant concern in fine motor function. Although CNS infections without associated encephalitis are generally assumed to be benign our study shows that at a median age of 18 months almost half of the EV-infected infants (48.3%) and 20% of HPeV-positive infants presented some developmental concern in the ASQ-3 test. We recommend monitor the neurological development of infants during the first years of life after HPeV CNS infection and especially after EV CNS infection, even in mild cases, for an early intervention and stimulation of psychomotor development if necessary.
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- 2021
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5. Enterovirus D68-associated respiratory and neurological illness in Spain, 2014–2018
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Rubén González-Sanz, Irene Taravillo, Jordi Reina, Ana Navascués, Antonio Moreno-Docón, Maitane Aranzamendi, María Pilar Romero, Margarita del Cuerpo, Carmen Pérez-González, Sonia Pérez-Castro, Almudena Otero, and María Cabrerizo
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Enterovirus ,enterovirus D68 ,respiratory infections ,acute flaccid paralysis ,phylogenetic analysis ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
ABSTRACTDuring 2014, enterovirus D68 (EV-D68) outbreaks were described globally, causing severe respiratory diseases in children and, in some cases, subsequent paralysis. In this study, the type characterization of enterovirus (EV) detected in respiratory illnesses and the epidemiology and clinical association of EV-D68 infections in Spain over a five-year period were described. A total of 546 EV-positive samples from hospitalized patients with respiratory infections were included. EV-D68 was the most frequently detected type (46.6%, 191/410 typed EV). Other EV from species A (25.1%), B (27.8%) and C (0.5%) were also identified. EV-D68 infections were more associated with bronchitis while EV-A/B types were more frequent in upper respiratory illness (p < 0.01). EV-D68 was also detected in patients with neurological symptoms (nine meningitis/meningoencephalitis and eight acute flaccid paralysis cases). Phylogenetic analysis of 3′-VP1 region showed most Spanish EV-D68 sequences from 2014 to 2016 belonged to subclades B2/B3, as other American and European strains circulating during the same period. However, those detected in 2017 and 2018 clustered to the emerged subclade D1. In summary, different EV can cause respiratory infections but EV-D68 was the most prevalent, with several strains circulating in Spain at least since 2014. Association between EV-D68 infection and neurological disease was also described.
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- 2019
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6. Epidemiology of Echovirus 30 Infections Detected in a University Hospital in Catalonia, Spain, in 1995–2020
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Margarita del Cuerpo, Jon Gonzalez de Audicana, Maria Dolores Fernandez-Garcia, Pilar Marín, Montserrat Esteban, Montserrat Español, María Cabrerizo, and Núria Rabella
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enteroviruses ,echovirus 30 ,central nervous system ,cerebrospinal fluid ,phylogenetic analysis ,epidemiology ,Biology (General) ,QH301-705.5 - Abstract
There is a growing interest in echovirus 30 (E30), an enterovirus responsible for neurological disease and hospitalization. There are multiple studies of outbreaks, but few that study the epidemiology over long periods of time. Our study aims to describe the clinical, epidemiological and microbiological characteristics of a series of E30 infections detected over 26 years. Data were retrospectively collected from a database of all enterovirus infections identified in our laboratory. They were detected by viral isolation or nucleic acid detection in patients presenting with respiratory or neurological infections, rash, sepsis-like syndrome, or gastroenteritis. Enterovirus genotyping was performed by amplification of the VP1 gene using RT-nested PCR, followed by sequencing and BLAST analysis. Of the 2402 enterovirus infections detected, 1619 were linked to at least one genotype and 173 were caused by E30. Clinical information was available for 158 (91.3%) patients. E30 was associated with neurological infection in 107 (67.8%) cases and it was detected almost every year. Phylogenetic analysis was performed with 67 sequences. We observed that E30 strains circulating in Catalonia from 1996 to 2016 belong to two lineages (E and F), although the majority cluster was in F. In 2018, lineage I emerged as the dominant lineage.
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- 2022
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7. Características epidemiológicas y clínicas de los lactantes hospitalizados por infecciones por parechovirus humanos. Estudio prospectivo en España
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Fernando Martín del Valle, Cristina Calvo, Inés Martinez-Rienda, Amaia Cilla, María P. Romero, Ana Isabel Menasalvas, Leticia Reis-Iglesias, Diana Roda, María J. Pena, Nuria Rabella, María del Mar Portugués de la Red, Gregoria Megías, Antonio Moreno-Docón, Almudena Otero, and María Cabrerizo
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Human parechovirus ,Infants ,Fever of unknown origin ,Clinical sepsis ,Pediatrics ,RJ1-570 - Abstract
Resumen: Introducción: Los parechovirus humanos (HPeV) son virus de la familia Picornaviridae, recientemente descritos, a los que se atribuyen cuadros de fiebre sin foco (FSF), sepsis clínica, gastroenteritis, meningitis o encefalitis fundamentalmente en lactantes pequeños. Nuestro objetivo fue describir la epidemiología y las características clínicas de las infecciones por HPeV en nuestro medio. Pacientes y métodos: Estudio multicéntrico prospectivo, llevado a cabo en 12 hospitales a nivel nacional, entre 2013-2015, en niños 3 meses. Todos los pacientes presentaron fiebre, el 45% irritabilidad, el 18,6% exantema y el 14% diarrea. No se observa ninguna alteración específica en las pruebas bioquímicas. El diagnóstico final más frecuente fue FSF (61%) seguido de sepsis clínica (29%). Aunque un 29% de los niños precisaron ingreso en cuidados intensivos, solo un paciente presentó secuelas. Conclusiones: Los HPeV circulan en nuestro país, afectando fundamentalmente a lactantes < 2 meses y se asocian a FSF y sepsis clínica, con un predominio en primavera y verano. Sería de interés implementar las técnicas moleculares de diagnóstico en todos los hospitales para reconocer y manejar adecuadamente estas infecciones. Abstract: Introduction: Human parechovirus (HPeV) is one of the recently described picornaviridae viruses that have been associated with fever of unknown origin (FUO), clinical sepsis, gastroenteritis, meningitis, or encephalitis in very young infants. The aim of this study is to describe the epidemiology and clinical features of these viruses. Patients and methods: A prospective multicentre 3-year study was conducted in 12 hospitals in Spain. Out of 850 specimens examined, 47 were positive (5.52%), with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FUO (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae. Results: Out of 850 specimens examined, 47 were positive (5.52%) for HPeV, with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FUO (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae Conclusions: HPeV circulates in our country, mainly during spring and summer, and affects young infants with a FUO and clinical sepsis. Molecular diagnostic techniques in all hospitals could help in improving the management of patients with these infections.
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- 2018
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8. Epidemiological and clinical characteristics of infants admitted to hospital due to human parechovirus infections: A prospective study in Spain
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Fernando Martín del Valle, Cristina Calvo, Inés Martinez-Rienda, Amaia Cilla, María P. Romero, Ana Isabel Menasalvas, Leticia Reis-Iglesias, Diana Roda, María J. Pena, Nuria Rabella, María del Mar Portugués de la Red, Gregoria Megías, Antonio Moreno-Docón, Almudena Otero, and María Cabrerizo
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Parechovirus humano ,Lactante ,Fiebre sin foco ,Sepsis ,Pediatrics ,RJ1-570 - Abstract
Introduction: Human parechovirus (HPeV) is one of the recently described picornaviridae viruses that have been associated with fever without source (FWS), clinical sepsis, gastroenteritis, meningitis, or encephalitis in very young infants. The aim of this study is to describe the epidemiology and clinical features of these viruses. Patients and methods: A prospective multicentre 3-year study was conducted in 12 hospitals in Spain. Out of 850 specimens examined, 47 were positive (5.52%), with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FWS (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae. Results: Out of 850 specimens examined, 47 were positive (5.52%) for HPeV, with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FWS (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae. Conclusions: HPeV circulates in our country, mainly during spring and summer, and affects young infants with a FWS and clinical sepsis. Molecular diagnostic techniques in all hospitals could help in improving the management of patients with these infections. Resumen: Introducción: Los parechovirus humanos (HPeV) son virus de la familia Picornaviridae, recientemente descritos, a los que se atribuyen cuadros de fiebre sin foco (FSF), sepsis clínica, gastroenteritis, meningitis o encefalitis fundamentalmente en lactantes pequeños. Nuestro objetivo fue describir la epidemiología y las características clínicas de las infecciones por HPeV en nuestro medio. Pacientes y métodos: Estudio multicéntrico prospectivo, llevado a cabo en 12 hospitales a nivel nacional, entre 2013-2015, en niños 3 meses. Todos los pacientes presentaron fiebre, el 45% irritabilidad, el 18,6% exantema y el 14% diarrea. No se observa ninguna alteración específica en las pruebas bioquímicas. El diagnóstico final más frecuente fue FSF (61%) seguido de sepsis clínica (29%). Aunque un 29% de los niños precisaron ingreso en cuidados intensivos, solo un paciente presentó secuelas. Conclusiones: Los HPeV circulan en nuestro país, afectando fundamentalmente a lactantes < 2 meses y se asocian a FSF y sepsis clínica, con un predominio en primavera y verano. Sería de interés implementar las técnicas moleculares de diagnóstico en todos los hospitales para reconocer y manejar adecuadamente estas infecciones.
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- 2018
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9. Author Correction: Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
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Fabiana Gámbaro, Ana Belén Pérez, Eduardo Agüera, Matthieu Prot, Luis Martínez-Martínez, María Cabrerizo, Etienne Simon-Loriere, and Maria Dolores Fernandez-Garcia
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Medicine ,Science - Published
- 2021
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10. Lower Respiratory Tract Infection and Genus Enterovirus in Children Requiring Intensive Care: Clinical Manifestations and Impact of Viral Co-Infections
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Daniel Penela-Sánchez, Jon González-de-Audicana, Georgina Armero, Desiree Henares, Cristina Esteva, Mariona-Fernández de-Sevilla, Silvia Ricart, Iolanda Jordan, Pedro Brotons, María Cabrerizo, Carmen Muñoz-Almagro, and Cristian Launes
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rhinovirus ,enterovirus ,co-infection ,intensive care units ,child ,lower respiratory tract infection ,Microbiology ,QR1-502 - Abstract
Infection by rhinovirus (RV) and enterovirus (EV) in children ranges from asymptomatic infection to severe lower respiratory tract infection (LRTI). This cohort study evaluates the clinical impact of RV/EV species, alone or in codetection with other viruses, in young children with severe LRTI. Seventy-one patients aged less than 5 years and admitted to the Paediatric Intensive Care Unit (PICU) of a reference children’s hospital with RV or EV (RV/EV) LRTI were prospectively included from 1/2018 to 3/2020. A commercial PCR assay for multiple respiratory pathogens was performed in respiratory specimens. In 22/71, RV/EV + respiratory syncytial virus (RSV) was found, and 18/71 had RV/EV + multiple viral detections. Patients with single RV/EV detection required invasive mechanical ventilation (IMV) as frequently as those with RSV codetection, whereas none of those with multiple viral codetections required IMV. Species were determined in 60 samples, 58 being RV. No EV-A, EV-C, or EV-D68 were detected. RV-B and EV-B were only found in patients with other respiratory virus codetections. There were not any associations between RV/EV species and severity outcomes. To conclude, RV/EV detection alone was observed in young children with severe disease, while multiple viral codetections may result in reduced clinical severity. Differences in pathogenicity between RV and EV species could not be drawn.
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- 2021
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11. Surveillance for Enteroviruses Associated with Hand, Foot, and Mouth Disease, and Other Mucocutaneous Symptoms in Spain, 2006–2020
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Nieves Martínez-López, Carmen Muñoz-Almagro, Cristian Launes, Ana Navascués, Manuel Imaz-Pérez, Jordi Reina, María Pilar Romero, Cristina Calvo, Montserrat Ruiz-García, Gregoria Megias, Juan Valencia-Ramos, Almudena Otero, and María Cabrerizo
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exanthema ,genotyping ,coxsackievirus A6 ,enterovirus A71 ,phylogenetic analysis ,Microbiology ,QR1-502 - Abstract
Hand, foot, and mouth disease (HFMD) is a mild illness caused by enteroviruses (EV), although in some Asian countries, large outbreaks have been reported in the last 25 years, with a considerable incidence of neurological complications. This study describes epidemiological and clinical characteristics of EV infections involved in HFMD and other mucocutaneous symptoms from 2006 to 2020 in Spain. EV-positive samples from 368 patients were included. EV species A were identified in 85.1% of those typed EV. Coxsackievirus (CV) A6 was the prevalent serotype (60.9%), followed by EV-A71 (9.9%) and CVA16 (7.7%). Infections affected children (1–6 years old) mainly, and show seasonality with peaks in spring–summer and autumn. Clinical data indicated few cases of atypical HFMD as well as those with neurological complications (associated with the 2016 EV-A71 outbreak). Phylogenetic analysis of CVA6 VP1 sequences showed different sub-clusters circulating from 2010 to present. In conclusion, HFMD or exanthemas case reporting has increased in Spain in recent years, probably associated with an increase in circulation of CVA6, although they did not seem to show greater severity. However, EV surveillance in mucocutaneous manifestations should be improved to identify the emergence of new types or variants causing outbreaks and more severe pathologies.
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- 2021
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12. BROTES EPIDÉMICOS Y SITUACIONES DE ALERTA SANITARIA DE PROBABLE ETIOLOGÍA VÍRICA ESTUDIADOS EN EL CENTRO NACIONAL DE MICROBIOLOGÍA DURANTE EL PERÍODO 2012-2013
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José Manuel Echevarría Mayo, Ana Avellón Calvo, María Cabrerizo Sanz, Inmaculada Casas Flecha, Juan Emilio Echevarría Mayo, Fernando de Ory Manchón, Anabel Negredo Antón, Francisco Pozo Sánchez, María Paz Sánchez-Seco Fariñas, David Tarragó Asensio, and Gloria Trallero Masó
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Medicine ,Public aspects of medicine ,RA1-1270 - Published
- 2016
13. La importancia de la vigilancia epidemiológica de los enterovirus emergentes
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Alfonso Amado Puentes and María Cabrerizo Sanz
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Pediatrics ,RJ1-570 - Published
- 2019
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14. PAPEL DEL LABORATORIO NACIONAL DE POLIOVIRUS EN EL PROGRAMA DE ERRADICACIÖN Y VIGILANCIA DE LA POLIOMIELITIS
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Gloria Trallero, María Cabrerizo, Ana Avellón, and Red de Laboratorios de Vigilancia de Parálisis Flácida Aguda
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Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
El Laboratorio Nacional de Poliovirus (LNP) coordina la Red Española de Vigilancia de Parálisis Flácida Aguda desde 1998 y caracteriza los poliovirus (PV) y otros enterovirus detectados, utilizando métodos de cultivo celular y moleculares. Durante 1998-2012 se estudiaron por la Red un total de 110.725 (70.046+40.679) muestras clínicas, resultando positivas para enterovirus 8.804 (8%), entre las que 241 se caracterizaron como PV. La caracterización intratípica demostró que todos los PV eran vacunales excep- to las muestras correspondientes a un caso importado de poliomielitis postvacunal y sus contactos, que fueron caracterizados como PV2 derivado de vacuna. En el LNP se ha realizado el serotipado y la caracterización intratípica de todos los PV aislados en España de cualquier síndrome. Con ello se ha demostrado que el PV salvaje no ha circulado en nuestro país durante los 15 años que recoge este trabajo y eso condujo a la firma del Acta de la “Erradicación de la Poliomielitis en España” por parte de la OMS en 2001 y a la “Certificación de la Erradicación Europea como libre de circulación de PV salvaje”el 21 de junio de 2002 . En la actualidad sólo 3 países presentan transmisión endémica de PV salvaje (Pakistán, Afganistán y Nigeria) y hasta que no se haya conseguido la erradicación a nivel mundial, España debe mantener la infraestructura creada en el Plan de Erradicación de la Poliomielitis y continuar con la vigilancia e inmunización. También el Programa de Contención de los PV salvajes en los laboratorios debe seguir en activo para evitar reintroducciones accidentales.
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- 2013
15. Invasive bacterial infection in children with fever and petechial rash in the emergency department: a national prospective observational study
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Pilar Storch-De-Gracia, Jose Luis Fernández, Roberto Velasco, Itsaso Saez, Rocío Rodrigo, Sandra Yañez, Ester Castellarnau, Elena Gil, Patricia del Rio, Estíbaliz Garrido, Antón Castaño, Álvaro Perez, María Cabrerizo, María Hernández, Juan Jesús Pérez, María José de la Torre, Gemma Nadal, Javier Martínez, and Victoria Sánchez-Tatay
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Pediatrics, Perinatology and Child Health - Abstract
ObjectiveTo determine the incidence and clinical predictors of invasive bacterial infection (IBI) in well-appearing children who present to the emergency department (ED) with fever and petechiae.DesignA prospective, observational, multicentre study was conducted in 18 hospitals between November 2017 and October 2019.PatientsA total of 688 patients were recruited.Main outcome measuresThe primary outcome was the presence of IBI. Clinical features and laboratory test results were described and related to the presence of IBI.ResultsTen IBIs were found (1.5%), comprising eight cases of meningococcal disease and two of occult pneumococcal bacteraemia. Median age was 26.2 months (IQR 15.3–51.2). Blood samples were obtained from 575 patients (83.3%). Patients with an IBI had a shorter time from fever to ED visit (13.5 hours vs 24 hours) and between fever and rash onset (3.5 hours vs 24 hours). Values for absolute leucocyte count, total neutrophil count, C reactive protein and procalcitonin were significantly higher in patients with an IBI. Significantly fewer patients with a favourable clinical status while in the observation unit were found to have an IBI (2/408 patients, 0.5%) than when clinical status was unfavourable (3/18, 16.7%).ConclusionsThe incidence of IBI among children with fever and petechial rash is lower than previously reported (1.5%). The time from fever to ED visit and to rash onset was shorter in patients with an IBI. Patients with a favourable clinical course during observation in the ED are at lower risk of IBI.
- Published
- 2023
16. Impact of the bacterial nasopharyngeal microbiota on the severity of genus enterovirus lower respiratory tract infection in children: a case–control study
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Daniel Penela-Sánchez, Muntsa Rocafort, Desiree Henares, Iolanda Jordan, Pedro Brotons, María Cabrerizo, Cristian Launes, Carmen Muñoz-Almagro, Instituto de Salud Carlos III, and Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
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Pulmonary and Respiratory Medicine ,Unidades de cuidados intensivos ,Intensive care units ,Bacteria ,Rhinovirus ,Infecció del tracte respiratori inferior ,Microbiota ,Infant ,Infección del tracto respiratorio inferior ,Lower respiratory tract infection ,Cross-Sectional Studies ,Case-Control Studies ,RNA, Ribosomal, 16S ,Pediatrics, Perinatology and Child Health ,Viruses ,Enterovirus Infections ,Humans ,Child ,Respiratory Tract Infections ,Unitats de cures intensives ,Rinovirus ,Enterovirus - Abstract
Introduction: Rhinoviruses (RV) and enteroviruses (EV) are among the main causative etiologies of lower respiratory tract infection (LRTI) in children. The clinical spectrum of RV/EV infection is wide, which could be explained by diverse environmental, pathogen-, and host-related factors. Little is known about the nasopharyngeal microbiota as a risk factor or disease modifier for RV/EV infection in pediatric patients. This study describes distinct nasopharyngeal microbiota profiles according to RV/EV LRTI status in children. Methods: Cross-sectional case-control study, conducted at Hospital Sant de Déu (Barcelona, Spain) from 2017 to 2020. Three groups of children
- Published
- 2023
17. Asociación entre penfigoide ampolloso y los inhibidores de la dipeptidil peptidasa-4, e impacto de su retirada
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David Martín-Enguix, Ricardo Ruiz-Villaverde, Manuel Galán-Gutiérrez, and Ana María Cabrerizo-Carvajal
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General Medicine ,Family Practice - Published
- 2023
18. Acute flaccid paralysis and enterovirus in Spain. Results from 2019 surveillance
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S. Sanbonmatsu, V. Gallardo-García, A. Nieto Juliá, Josefa Masa-Calles, I. Huerta, Antonio Moreno-Docón, D Castrillejo, S. Pérez-Castro, Nuria Rabella, María Pilar Romero, A. Blasco, V. García-Ortúzar, A. Blanco, Nuria Torner, S. García-Hernández, M. del Cuerpo, J.M. Navarro, Isabel Losada, Carmen Muñoz-Almagro, Carmen Pérez-González, María Cabrerizo, F. González Carril, Carmen Malo, C. Marín, M. García Cenoz, Conchita Izquierdo, Henar Marcos, A. Rivas, M. Portell, M.L. Rojo, Maitane Aranzamendi, Ana Navascués, Noemí López-Perea, A. Martínez-Sapiña, and José Ramos
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Acute flaccid paralysis ,Pediatrics ,medicine.medical_specialty ,business.industry ,Poliovirus ,General Medicine ,medicine.disease ,medicine.disease_cause ,World health ,Poliomyelitis ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,medicine ,Enterovirus ,030212 general & internal medicine ,business ,Notification rate - Abstract
Objective The Acute Flaccid Paralysis (AFP) and Enterovirus (EV) Surveillance systems are key to achieving the eradication of poliomyelitis worldwide. Among others, they allow detecting the reintroduction of poliovirus (PV) from endemic areas. Material and methods Analysis of the results of the protocol for the surveillance of AFPs and EVs in 2019 and the standard quality index established by the World Health Organization. Results 38 cases of AFP were reported, -notification rate of 0.55 / 100,000 Conlusions As long as there is circulation of poliovirus in the world, it is necessary to keep the surveillance systems of EV -polio and nonpolio- active, reinforcing the collection and processing of samples to detect the unexpected circulation of these viruses in time.
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- 2021
19. Parálisis flácida aguda y enterovirus en España. Resultados de la vigilancia en 2019
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Isabel Losada, Maitane Aranzamendi, V. García-Ortúzar, A. Martínez-Sapiña, Josefa Masa-Calles, M. del Cuerpo, José Ramos, A. Nieto Juliá, S. Sanbonmatsu, S. Pérez-Castro, María Pilar Romero, A. Blasco, Nuria Torner, Conchita Izquierdo, F. González Carril, C. Marín, A. Blanco, Ana Navascués, Antonio Moreno-Docón, M. Portell, Nuria Rabella, M.L. Rojo, Henar Marcos, D Castrillejo, Carmen Malo, Noemí López-Perea, A. Rivas, M. García Cenoz, J.M. Navarro, V. Gallardo-García, Carmen Pérez-González, I. Huerta, S. García-Hernández, Carmen Muñoz-Almagro, and María Cabrerizo
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03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,030225 pediatrics ,Immunology ,030212 general & internal medicine - Abstract
Resumen Objetivo Los sistemas de Vigilancia de Paralisis Flacida Aguda (PFA) y Enterovirus (EV) son clave para lograr la erradicacion de la poliomielitis en todo el mundo. Entre otras, permiten detectar la reintroduccion de poliovirus (PV) desde zonas endemicas. Material y metodos Analisis de los resultados del protocolo para la vigilancia de las PFA y EV en 2019 y el indice de calidad estandar establecido por la Organizacion Mundial de la Salud (OMS). Resultados En Espana en 2019 se notificaron 38 casos de PFA —tasa de notificacion de 0,55/100.000 Conclusiones Mientras haya circulacion de poliovirus en el mundo, es necesario mantener activos los sistemas de vigilancia de EV —polio y no polio— reforzando la recogida y el procesamiento de muestras para detectar a tiempo la circulacion inesperada de dichos virus.
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- 2021
20. Author Correction: Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
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Ana Belén Pérez, Fabiana Gámbaro, Luis Martínez-Martínez, María Cabrerizo, Etienne Simon-Loriere, Maria Dolores Fernandez-Garcia, Eduardo Agüera, and Matthieu Prot
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Pediatrics ,medicine.medical_specialty ,Multidisciplinary ,business.industry ,Science ,MEDLINE ,Aseptic meningitis ,medicine.disease_cause ,medicine.disease ,Medicine ,Enterovirus ,business - Published
- 2021
21. Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015-2018
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Luis Martínez-Martínez, Matthieu Prot, María Cabrerizo, Ana Belén Pérez, Eduardo Agüera, Fabiana Gámbaro, Etienne Simon-Loriere, Maria Dolores Fernandez-Garcia, Regional Government of Andalusia (España), Instituto de Salud Carlos III, Nvidia Corporation, Institut Pasteur [Paris] (IP), Hospital Universitario Reina Sofia [Cordoue, Espagne], Instituto Maimonides de Investigación Biomédica de Cordoba (IMIBIC), Universidad de Córdoba = University of Córdoba [Córdoba]-Hospital Universitario Reina Sofía, Universidad de Córdoba = University of Córdoba [Córdoba], Instituto de Salud Carlos III [Madrid] (ISC), CIBER de Epidemiología y Salud Pública (CIBERESP), IdiPAZ - Instituto de Investigación La Paz [Madrid, Spain], This work was funded by the Junta de Andalucía, Consejería de Salud y Familias (Project number PI-0216-2019) and by the Acción Estratégica en Salud Intramural (Project number PI20CIII/00005). MD Fernandez-Garcia received a Miguel Servet Research Contract (CP18/00067) from the Strategic Action in Health 2018 and funded by National Institute of Health Carlos III (ISCIII). ESL acknowledges funding from the INCEPTION programme (Investissements d’Avenir grant ANR-16-CONV-0005)., ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), [Gámbaro,F, Prot,M, Simon-Loriere,E] Institut Pasteur, Paris, France. [Pérez,AB, Agüera,E, Martínez-Martínez,L] Hospital Universitario Reina Sofía, Córdoba, Spain. [Pérez,AB, Martínez-Martínez,L, and Fernandez-Garcia,MD] Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain. [Martínez-Martínez,L] Universidad de Córdoba, Córdoba, Spain. [Cabrerizo,M] National Centre for Microbiology, Instituto de Salud Carlos III, Madrid, Spain. [Cabrerizo,M] CIBER de epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [Cabrerizo,M] Red de Investigación Translacional en Infectología Pediátrica (RITIP), IdiPaz, Madrid, Spain.
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Male ,Echovirus ,España ,medicine.disease_cause ,Genome ,law.invention ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Human disease ,law ,Meningitis, Aseptic ,Phylogeny ,Enterovirus ,Multidisciplinary ,Aseptic meningitis ,Genomics ,Enterovirus B, Human ,ARN ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Recombinant DNA ,Medicine ,RNA, Viral ,Female ,Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings] ,Persons::Persons::Age Groups::Adult::Young Adult [Medical Subject Headings] ,Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome, Microbial::Genome, Viral [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA [Medical Subject Headings] ,Adult ,Adolescent ,Genotype ,Genómica ,Science ,Check Tags::Male [Medical Subject Headings] ,Genome, Viral ,Biology ,Microbiology ,Article ,Persons::Persons::Age Groups::Adolescent [Medical Subject Headings] ,Young Adult ,Virology ,medicine ,Enterovirus Infections ,Humans ,Meningitis ,Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Viral [Medical Subject Headings] ,Persons::Persons::Age Groups::Adult [Medical Subject Headings] ,Author Correction ,Geographical Locations::Geographic Locations::Europe::Spain [Medical Subject Headings] ,Outbreak ,Sequence Analysis, DNA ,medicine.disease ,Diseases::Virus Diseases::RNA Virus Infections::Picornaviridae Infections::Enterovirus Infections [Medical Subject Headings] ,Check Tags::Female [Medical Subject Headings] ,Metagenomics ,Spain ,Diseases::Nervous System Diseases::Central Nervous System Diseases::Central Nervous System Infections::Meningitis::Meningitis, Aseptic [Medical Subject Headings] ,RNA ,Organisms::Viruses::RNA Viruses::Picornaviridae::Enterovirus::Enterovirus B, Human [Medical Subject Headings] ,Phenomena and Processes::Genetic Phenomena::Phylogeny [Medical Subject Headings] ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
Author Correction: Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015-2018. Sci Rep. 2021 Nov 18;11(1):22899. doi: 10.1038/s41598-021-02194-2. New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs. This work was funded by the Junta de Andalucía, Consejería de Salud y Familias (Project number PI-0216-2019) and by the Acción Estratégica en Salud Intramural (Project number PI20CIII/00005). MD Fernandez-Garcia received a Miguel Servet Research Contract (CP18/00067) from the Strategic Action in Health 2018 and funded by National Institute of Health Carlos III (ISCIII). ESL acknowledges funding from the INCEPTION programme (Investissements d’Avenir grant ANR-16-CONV-0005). Sí
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- 2021
22. Circulation of non-polio enteroviruses in 24 EU and EEA countries between 2015 and 2017: a retrospective surveillance study
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Natasa Berginc, Peter Simmonds, Heli Harvala, Trung Nguyen, Aftab Jasir, Ursula Morley, Rubén González-Sanz, Guôrún Erna Baldvinsdóttir, Jevgenia Epstein, Laura Pellegrinelli, Katherina Zakikhany, Kate Templeton, Oksana Martinuka, Petra Rainetova, Elenor Hauzenberger, Susan Feeney, Sofie Midgley, Simon Cottrell, Sanela Numanovic, Shubhada Bopegamage, María Cabrerizo, Antonio Piralla, Jake Dunning, Susanne Gjeruldsen Dudman, Kimberley S. M. Benschop, Sindy Böttcher, Agnes Farkas, Laura Bubba, Tecu Cristina, Thea Kølsen Fischer, Monika Redlberger-Fritz, Catherine Moore, Soile Blomqvist, Eeva Broberg, Martina Havlíčková, Kathrin Keeren, Cillian De Gascun, Natālija Zamjatina, Algirdas Griskevicius, Cristina Celma, Raquel Guiomar, James McKenna, Magdalena Wieczorek, Inês Costa, Lubomira Nikolaeva-Glomb, Katarina Pastuchova, Erika Bujaki, and Erwin Duizer
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0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,Myocarditis ,Echovirus ,Genotype ,viruses ,030106 microbiology ,Disease ,Coxsackievirus ,medicine.disease_cause ,03 medical and health sciences ,Epidemiology ,medicine ,Enterovirus Infections ,Humans ,Typing ,Demography ,Enterovirus ,Retrospective Studies ,Molecular Epidemiology ,biology ,business.industry ,virus diseases ,Sequence Analysis, DNA ,medicine.disease ,biology.organism_classification ,Survival Analysis ,3. Good health ,Poliomyelitis ,Europe ,030104 developmental biology ,Infectious Diseases ,Epidemiological Monitoring ,Capsid Proteins ,business - Abstract
Summary Background Enteroviruses can cause severe infections, especially in young children. Non-polio enterovirus infections are not notifiable in most countries in the EU and European Economic Area (EEA) region, and surveillance varies substantially between countries. We collected and analysed available enterovirus data across EU and EEA countries to assess the current epidemiological situation and need for standardising surveillance. Methods Aggregated data on any enterovirus detected between Jan 1, 2015, and Dec 31, 2017, through national enterovirus reference laboratories were requested from representatives in all 31 EU and EEA countries. Information collected included enterovirus types detected by month, patient age group, symptom, and specimen type. We also collected sequence data on viral capsid sequences for the three most clinically relevant enterovirus types, as identified from the data. Findings Aggregated data were provided by representatives from 24 (77%) of 31 countries. 9914 (66%) of 14 999 enterovirus infections with information about age were in children younger than 5 years, and 3197 (45%) of 7139 individuals for whom symptoms were reported had neurological symptoms. Other symptoms were non-specific fever (in 1607 [23%] patients), respiratory symptoms (in 1197 [17%] patients), hand, foot, and mouth disease (in 528 [7% patients), and myocarditis (in 39 [1%] patients). 68 deaths were temporally associated with enterovirus infection. Typing for 11 559 (67%) of 17 136 specimens revealed 66 enterovirus types. Coxsackievirus A6 was the most frequently detected enterovirus type (in 1556 [13%] of 11 559 typed enteroviruses), and 292 (65%) of 448 patients with coxsackievirus A6 infection with available clinical data presented with hand, foot, and mouth disease. Echovirus 30 was the second most frequently detected enterovirus type, representing 1412 (12%) of 11 559 typed enteroviruses, and 384 (82%) of 467 individuals with echovirus 30 infection with available clinical data had neurological symptoms. Sequences available from 18 countries showed circulation of newly emerging strains of enterovirus A71 and enterovirus D68. Interpretation To our knowledge, this study is the largest investigation of enterovirus circulation in EU and EEA countries and confirms the availability of non-polio enterovirus data in the region. Our study highlights the wide circulation of non-polio enteroviruses in Europe, mostly affecting young children and leading to neurological symptoms. Collecting data on morbidity and mortality related to enterovirus infections, as well as harmonising case definition for surveillance, should be encouraged. Funding None.
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- 2021
23. Cerebrospinal Fluid Neopterin in Children With Enterovirus-Related Brainstem Encephalitis
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Claudia Fortuny, María Cabrerizo, Ana Valero-Rello, Iolanda Jordan, Thaís Armangue, Marta Molero-Luis, Dídac Casas-Alba, Cristian Launes, Jordi Muchart, Carmen Muñoz-Almagro, and Rafael Artuch
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Male ,medicine.medical_specialty ,Encephalomyelitis ,Myelitis ,medicine.disease_cause ,Neopterin ,Gastroenterology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cerebrospinal fluid ,Developmental Neuroscience ,Interquartile range ,030225 pediatrics ,Internal medicine ,Enterovirus Infections ,medicine ,Humans ,Encephalitis, Viral ,Prospective Studies ,Prospective cohort study ,business.industry ,Infant ,medicine.disease ,Magnetic Resonance Imaging ,Enterovirus A, Human ,Neurology ,chemistry ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Enterovirus ,Biomarker (medicine) ,Female ,Neurology (clinical) ,business ,030217 neurology & neurosurgery ,Brain Stem - Abstract
Background Enterovirus-A71 causes outbreaks of brainstem encephalitis, ranging from self-limited disease to acute flaccid paralysis. The aim of this study was to assess the role of cerebrospinal fluid (CSF) neopterin as a biomarker of disease severity in children with enterovirus-related brainstem encephalitis. Methods A descriptive, prospective cohort study was conducted from April 2016 to March 2017 in a tertiary hospital. Pediatric patients with a diagnosis of brainstem encephalitis with or without myelitis due to enterovirus infection were enrolled. The final study group comprised a convenience sample including all patients with sufficient CSF volume for neopterin determination. The major variables considered in estimating the severity were the diagnosis of encephalomyelitis, the presence of lesions and extensive lesions on brain and spinal magnetic resonance imaging (MRI), hospital stay length greater than seven days, and sequelae at day 30. Results Of 60 patients, CSF neopterin could be measured in 36. Median age was 26 months (interquartile range: 19 to 32). Thirty-three were diagnosed with brainstem encephalitis and three with encephalomyelitis. Enterovirus-A71 was the only identified genotype (25 of 25). CSF neopterin levels were elevated (>61 nmol/L) in 33 of 36 (92%), with a median of 347 nmol/L (interquartile range: 204 to 525). CSF neopterin was useful to distinguish patients with lesions on MRI (area under the receiver operating characteristic curve = 0.76; P = 0.02) and extensive lesions (area under the receiver operating characteristic curve = 0.76; P = 0.04). Conclusions This study suggests an association between CSF neopterin levels and the presence of inflammatory lesions on MRI.
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- 2019
24. Neurodevelopment medium-term outcome after parechovirus infection
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Amaia Cilla, Cristina Calvo, Fernando Martín del Valle, María Cabrerizo Sanz, Ana Velázquez González, Ana Menasalvas Ruiz, and María de Ceano Vivas
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Male ,Pediatrics ,medicine.medical_specialty ,Developmental Disabilities ,Gross motor skill ,Motor function ,Medium term ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,medicine ,Humans ,Picornaviridae Infections ,biology ,business.industry ,Human parechovirus ,Infant ,Obstetrics and Gynecology ,Prognosis ,medicine.disease ,biology.organism_classification ,Hypotonia ,Paresis ,Hemiparesis ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Parechovirus ,Muscle Hypotonia ,Female ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Encephalitis - Abstract
Aim Human parechoviruses (HPeV) are responsible for fever without a source (FWS), sepsis-like illness and encephalitis in neonates and children under 3 months of age. Short-term outcome is generally good, but there is great concern about medium and long- term outcome of infants after HPeV infection. The aim of this study is to assess the medium-term outcome in infants following HPeV infection without encephalitis. Methods Patients who suffered HPeV infection involving cerebrospinal fluid were evaluated twice using Ages and Stages Questionnaire-3 (ASQ-3). The first evaluation was conducted at least one year after the infection and the second one year later. Results Sixteen patients were evaluated in the first assessment, and three of them presented mild alterations in motor function domains. Moreover, hypotonia was observed in the neurologic exam in one case, and hemiparesis in another case. In the second assessment fifteen patients were included, and only the patient with hemiparesis continued presenting gross motor disfunction, with complete recovery of the remaining patients. Interpretation We have observed a good medium-term prognosis in infants after HPeV infections, with improvement of mild motor alterations after at-home intervention. Infants who suffer HPeV infection without encephalitis seem to have a better prognosis than those with encephalitis.
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- 2019
25. Surveillance for Enteroviruses Associated with Hand, Foot, and Mouth Disease, and Other Mucocutaneous Symptoms in Spain, 2006-2020
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Cristian Launes, Cristina Calvo, Juan Valencia-Ramos, Nieves Martínez-López, Gregoria Megias, Manuel Imaz-Pérez, María Pilar Romero, Montserrat Ruiz-García, Jordi Reina, María Cabrerizo, Carmen Muñoz-Almagro, Ana Navascués, and Almudena Otero
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0301 basic medicine ,Serotype ,Male ,Enterovirus A71 ,Disease ,Disease Outbreaks ,0302 clinical medicine ,Epidemiology ,030212 general & internal medicine ,Child ,Phylogeny ,Enterovirus ,Phylogenetic analysis ,biology ,Incidence (epidemiology) ,QR1-502 ,Infectious Diseases ,enterovirus A71 ,Child, Preschool ,Female ,exanthema ,Coxsackievirus A6 ,Seasons ,Foot (unit) ,medicine.medical_specialty ,Genotyping ,Adolescent ,Genotype ,030106 microbiology ,Mucocutaneous zone ,Coxsackievirus ,Serogroup ,Microbiology ,Article ,03 medical and health sciences ,Virology ,medicine ,Enterovirus Infections ,Humans ,coxsackievirus A6 ,Mucous Membrane ,business.industry ,phylogenetic analysis ,Infant, Newborn ,Outbreak ,Infant ,Exanthema ,biology.organism_classification ,Dermatology ,genotyping ,Spain ,business ,Hand, Foot and Mouth Disease - Abstract
Hand, foot, and mouth disease (HFMD) is a mild illness caused by enteroviruses (EV), although in some Asian countries, large outbreaks have been reported in the last 25 years, with a considerable incidence of neurological complications. This study describes epidemiological and clinical characteristics of EV infections involved in HFMD and other mucocutaneous symptoms from 2006 to 2020 in Spain. EV-positive samples from 368 patients were included. EV species A were identified in 85.1% of those typed EV. Coxsackievirus (CV) A6 was the prevalent serotype (60.9%), followed by EV-A71 (9.9%) and CVA16 (7.7%). Infections affected children (1–6 years old) mainly, and show seasonality with peaks in spring–summer and autumn. Clinical data indicated few cases of atypical HFMD as well as those with neurological complications (associated with the 2016 EV-A71 outbreak). Phylogenetic analysis of CVA6 VP1 sequences showed different sub-clusters circulating from 2010 to present. In conclusion, HFMD or exanthemas case reporting has increased in Spain in recent years, probably associated with an increase in circulation of CVA6, although they did not seem to show greater severity. However, EV surveillance in mucocutaneous manifestations should be improved to identify the emergence of new types or variants causing outbreaks and more severe pathologies.
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- 2021
26. Lower Respiratory Tract Infection and Genus Enterovirus in Children Requiring Intensive Care: Clinical Manifestations and Impact of Viral Co-Infections
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Desiree Henares, Mariona-Fernández de-Sevilla, Pedro Brotons, Carmen Muñoz-Almagro, Iolanda Jordan, María Cabrerizo, Jon González-de-Audicana, Cristina Esteva, Silvia Ricart, Cristian Launes, Georgina Armero, Daniel Penela-Sánchez, Instituto de Salud Carlos III, and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
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Male ,Critical Care ,Rhinovirus ,intensive care units ,medicine.disease_cause ,Microbiology ,Asymptomatic ,Article ,Virus ,Lower respiratory tract infection ,co-infection ,Virology ,Intensive care ,Enterovirus Infections ,medicine ,Humans ,Respiratory system ,Child ,Respiratory Tract Infections ,Enterovirus ,Enterovirus D, Human ,child ,Intensive care units ,Coinfection ,enterovirus ,business.industry ,Infant, Newborn ,Infant ,medicine.disease ,QR1-502 ,Co-infection ,Hospitalization ,rhinovirus ,Infectious Diseases ,Child, Preschool ,Respiratory Syncytial Virus, Human ,lower respiratory tract infection ,Respiratory virus ,Female ,medicine.symptom ,business - Abstract
Infection by rhinovirus (RV) and enterovirus (EV) in children ranges from asymptomatic infection to severe lower respiratory tract infection (LRTI). This cohort study evaluates the clinical impact of RV/EV species, alone or in codetection with other viruses, in young children with severe LRTI. Seventy-one patients aged less than 5 years and admitted to the Paediatric Intensive Care Unit (PICU) of a reference children's hospital with RV or EV (RV/EV) LRTI were prospectively included from 1/2018 to 3/2020. A commercial PCR assay for multiple respiratory pathogens was performed in respiratory specimens. In 22/71, RV/EV + respiratory syncytial virus (RSV) was found, and 18/71 had RV/EV + multiple viral detections. Patients with single RV/EV detection required invasive mechanical ventilation (IMV) as frequently as those with RSV codetection, whereas none of those with multiple viral codetections required IMV. Species were determined in 60 samples, 58 being RV. No EV-A, EV-C, or EV-D68 were detected. RV-B and EV-B were only found in patients with other respiratory virus codetections. There were not any associations between RV/EV species and severity outcomes. To conclude, RV/EV detection alone was observed in young children with severe disease, while multiple viral codetections may result in reduced clinical severity. Differences in pathogenicity between RV and EV species could not be drawn. This project is supported by the Spanish National Health Institute Carlos III (Grant id. PI17/349). DH received a grant for predoctoral training in research into Health by the Spanish National Health Institute Carlos III (project number: FI17/00248). DH also received a grant from Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC) for a research stay. The funders have not influenced the design or analysis, nor have they had any role in preparing the manuscript. Sí
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- 2021
27. An imported case of vaccine-derived poliovirus type 2, Spain in the context of the ongoing polio Public Health Emergency of International Concern, September 2021
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María Dolores Chirlaque López, María Cabrerizo, Bernardo R. Guzmán Herrador, Josefa Masa-Calles, María Ester Alarcón-Linares, Ana Allende, Esteban Aznar Cano, María Isabel Barranco Boada, Elena Cantero Gudino, Sonia Fernández-Balbuena, Ana Fernández Dueñas, María Dolores Fernández-García, Laura García Hernández, Visitación García Ortúzar, Noemí López-Perea, Eduardo Martínez-Salcedo, Antonio Moreno-Docón, María Ordobás Gavín, Inmaculada Rodero Garduño, Maria José Sierra Moros, Fernando Simón Soria, Aurora Limia Sánchez, and Berta Suárez Rodríguez
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Acute Flaccid Paralysis ,Epidemiology ,Public health emergency of international concern ,Vaccine-derived poliovirus ,digestive, oral, and skin physiology ,Public Health, Environmental and Occupational Health ,public health emergency of international concern ,Poliovirus ,Spain ,Poliovirus Vaccine, Oral ,Population Surveillance ,Virology ,eradication ,Humans ,Paralysis ,Public Health ,vaccine-derived poliovirus ,Child ,Rapid Communication ,Poliomyelitis ,Retrospective Studies ,Eradication - Abstract
The monthly retrospective search for unreported acute flaccid paralysis (AFP) cases conducted as a complementary component of the Spanish AFP surveillance system identified a case of AFP in a child admitted in Spain from Senegal during August 2021. Vaccine-derived poliovirus 2 was identified in the stool in September 2021. We present public health implications and response undertaken within the framework of the National Action Plan for Polio Eradication and the Public Health Emergency of International Concern.
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- 2021
28. Genomic and serologic characterization of enterovirus A71 brainstem encephalitis
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Emily D. Crawford, Carmen Muñoz-Almagro, Charles Langelier, Carly K. Cheung, Hannah A. Sample, Michael R. Wilson, Ryan D. Schubert, Dídac Casas-Alba, Kristoffer E. Leon, Joseph L. DeRisi, María Cabrerizo, John E. Pak, Lillian M. Khan, Ana Valero-Rello, Cristian Launes, Wesley Wu, Isobel A. Hawes, Kelsey C. Zorn, Prashanth S. Ramachandran, Akshaya Ramesh, National Multiple Sclerosis Society (Estados Unidos), American Academy of Neurology, William K Bowes Jr Foundation, Rachleff Family Foundation, NIH - National Institute of Neurological Disorders and Stroke (NINDS), United Cancer Support Foundation (Estados Unidos), CZ Biohub, Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Instituto de Salud Carlos III, and European Regional Development Fund
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0301 basic medicine ,Male ,Children -- Health and hygiene ,Serology ,Cohort Studies ,Medicine ,Encephalitis, Viral ,Viral ,Child ,Phylogeny ,Pediatric ,Reverse Transcriptase Polymerase Chain Reaction ,High-Throughput Nucleotide Sequencing ,Meningitis, Viral ,Reverse transcription polymerase chain reaction ,Serología ,Neurology ,Serologia ,Child, Preschool ,RNA, Viral ,Encephalitis ,Female ,Infection ,Meningitis ,Sequence Analysis ,Niños -- Salud e higiene ,Brainstem ,Human ,Cerebro ,030106 microbiology ,Enzyme-Linked Immunosorbent Assay ,Article ,Virus ,03 medical and health sciences ,Immune system ,Antigen ,Enterovirus Infections ,Humans ,Infants -- Salud i Higiene ,Genomes ,Preschool ,Genomas ,Tronc de l'encèfal ,Sequence Analysis, RNA ,business.industry ,Prevention ,Neurosciences ,Outbreak ,Infant ,Encefalitis ,medicine.disease ,Virology ,Enterovirus A, Human ,030104 developmental biology ,RNA ,Neurology (clinical) ,Enterovirus A ,business ,Brain Stem - Abstract
OBJECTIVE: In 2016, Catalonia experienced a pediatric brainstem encephalitis outbreak caused by enterovirus A71 (EV-A71). Conventional testing identified EV in the periphery but rarely in CSF. Metagenomic next-generation sequencing (mNGS) and CSF pan-viral serology (VirScan) were deployed to enhance viral detection and characterization. METHODS: RNA was extracted from the CSF (n = 20), plasma (n = 9), stool (n = 15), and nasopharyngeal samples (n = 16) from 10 children with brainstem encephalitis and 10 children with meningitis or encephalitis. Pathogens were identified using mNGS. Available CSF from cases (n = 12) and pediatric other neurologic disease controls (n = 54) were analyzed with VirScan with a subset (n = 9 and n = 50) validated by ELISA. RESULTS: mNGS detected EV in all samples positive by quantitative reverse transcription polymerase chain reaction (qRT-PCR) (n = 25). In qRT-PCR-negative samples (n = 35), mNGS found virus in 23% (n = 8, 3 CSF samples). Overall, mNGS enhanced EV detection from 42% (25/60) to 57% (33/60) (p-value = 0.013). VirScan and ELISA increased detection to 92% (11/12) compared with 46% (4/12) for CSF mNGS and qRT-PCR (p-value = 0.023). Phylogenetic analysis confirmed the EV-A71 strain clustered with a neurovirulent German EV-A71. A single amino acid substitution (S241P) in the EVA71 VP1 protein was exclusive to the CNS in one subject. CONCLUSION: mNGS with VirScan significantly increased the CNS detection of EVs relative to qRT-PCR, and the latter generated an antigenic profile of the acute EV-A71 immune response. Genomic analysis confirmed the close relation of the outbreak EV-A71 and neuroinvasive German EV-A71. A S241P substitution in VP1 was found exclusively in the CSF. Grants supporting this project include the National Multiple Sclerosis Society and the American Academy of Neurology award FAN-1608-25607 (R.D.S.), Clinical Research Training Scholarship P0534134 (P.S.R.), Sandler and William K. Bowes Jr Foundations (M.R.W., J.L.D., L.M.K., H.A.S., K.C.Z.), Rachleff Family Foundation (M.R.W.), and NINDS of the NIH under award K08NS096117 (M.R.W.) and F31NS113432 (K.E.L.). This study was partially supported by a grant from the Spanish National Health Institute [grant number PI15CIII-00020] and the European Regional Development Fund (FEDER funds). UCSF Biomedical Sciences Program (I.A.H., K.E.L.), UCSF Medical Scientist Training Program (K.E.L.), and the Chan Zuckerberg Biohub (J.E.P., W.W., C.K.C., J.L.D., E.D.C.) also supported this project. Sí
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- 2020
29. Myocardial fibrosis in arrhythmogenic cardiomyopathy: a genotype-phenotype correlation study
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Juan Jiménez-Jáimez, José Manuel Oyonarte Ramírez, Laura Fernández-Navarro, Elisa María Cabrerizo, Silvia López-Fernández, María Dolores García-Roa, Diego Segura-Rodríguez, Luis Tercedor, Antonio Campos, Francisco Bermúdez-Jiménez, Miguel A. Alvarez, Mercedes González-Molina, Miguel Alaminos, Daniel Durand-Herrera, Rosa del Carmen Flores Macías, and Víctor Carriel
- Subjects
Adult ,Male ,medicine.medical_specialty ,Cardiomyopathy ,desmin ,Contrast Media ,Magnetic Resonance Imaging, Cine ,Gadolinium ,030204 cardiovascular system & hematology ,Gene mutation ,Right ventricular cardiomyopathy ,cardiac magnetic resonance ,histology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Linear gingival erythema ,Fibrosis ,Internal medicine ,0502 economics and business ,Medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Systole ,Arrhythmogenic Right Ventricular Dysplasia ,Genetic Association Studies ,business.industry ,Myocardium ,05 social sciences ,General Medicine ,arrhythmogenic cardiomyopathy ,Middle Aged ,medicine.disease ,Arrhythmogenic right ventricular dysplasia ,late gadolinium enhancement ,Mutation ,Cardiology ,050211 marketing ,Myocardial fibrosis ,myocardial fibrosis ,Female ,Cardiology and Cardiovascular Medicine ,business ,Cardiomyopathies - Abstract
Aims Arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) is a life-threatening entity with a highly heterogeneous genetic background. Cardiac magnetic resonance (CMR) imaging can identify fibrofatty scar by late gadolinium enhancement (LGE). Our aim is to investigate genotype–phenotype correlation in ARVC/D mutation carriers, focusing on CMR-LGE and myocardial fibrosis patterns. Methods and results A cohort of 44 genotyped patients, 33 with definite and 11 with borderline ARVC/D diagnosis, was characterized using CMR and divided into groups according to their genetic condition (desmosomal, non-desmosomal mutation, or negative). We collected information on cardiac volumes and function, as well as LGE pattern and extension. In addition, available ventricular myocardium samples from patients with pathogenic gene mutations were histopathologically analysed. Half of the patients were women, with a mean age of 41.6 ± 17.5 years. Next-generation sequencing identified a potential pathogenic mutation in 71.4% of the probands. The phenotype varied according to genetic status, with non-desmosomal male patients showing lower left ventricular (LV) systolic function. LV fibrosis was similar between groups, but distribution in non-desmosomal patients was frequently located at the posterolateral LV wall; a characteristic LV subepicardial circumferential LGE pattern was significantly associated with ARVC/D caused by desmin mutation. Histological analysis showed increased fibrillar connective tissue and intercellular space in all the samples. Conclusion Desmosomal and non-desmosomal mutation carriers showed different morphofunctional features but similar LV LGE presence. DES mutation carriers can be identified by a specific and extensive LV subepicardial circumferential LGE pattern. Further studies should investigate the specificity of LGE in ARVC/D.
- Published
- 2020
30. Epidemiological and clinical characteristics of infants admitted to hospital due to human parechovirus infections: A prospective study in Spain
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María José Mellado Peña, María Cabrerizo, Nuria Rabella, Amaia Cilla, Gregoria Megias, Inés Martinez-Rienda, Leticia Reis-Iglesias, Diana Rodà, Cristina Calvo, María Pilar Romero, Antonio Moreno-Docón, Almudena Otero, María del Mar Portugués-de la Red, Ana Isabel Menasalvas, and Fernando Martín del Valle
- Subjects
Male ,0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,030106 microbiology ,Parechovirus ,RJ1-570 ,law.invention ,Sepsis ,03 medical and health sciences ,0302 clinical medicine ,law ,030225 pediatrics ,Management of Technology and Innovation ,Epidemiology ,Fiebre sin foco ,medicine ,Humans ,Prospective Studies ,Fever of unknown origin ,Picornaviridae Infections ,business.industry ,Human parechovirus ,Infant, Newborn ,Infant ,Lactante ,medicine.disease ,Parechovirus humano ,Rash ,Intensive care unit ,Hospitalization ,Spain ,Female ,medicine.symptom ,business ,Meningitis ,Encephalitis - Abstract
Introduction: Human parechovirus (HPeV) is one of the recently described picornaviridae viruses that have been associated with fever without source (FWS), clinical sepsis, gastroenteritis, meningitis, or encephalitis in very young infants. The aim of this study is to describe the epidemiology and clinical features of these viruses. Patients and methods: A prospective multicentre 3-year study was conducted in 12 hospitals in Spain. Out of 850 specimens examined, 47 were positive (5.52%), with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FWS (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae. Results: Out of 850 specimens examined, 47 were positive (5.52%) for HPeV, with HPeV-3 being the most frequent (29 cases). Infections occurred throughout the year, but mainly in May and July, and a biennial distribution was observed. More than half (57%) were neonates, and only 2 children were older than 3 months. Fever was present in all children, with irritability in 45%, rash in 18.6%, and diarrhoea in 14%. The results of biochemical tests were all in normal range. The most common final diagnosis was FWS (61%), followed by clinical sepsis (29%). Up to 29% of infants were admitted to the intensive care unit, but only one patient had sequelae. Conclusions: HPeV circulates in our country, mainly during spring and summer, and affects young infants with a FWS and clinical sepsis. Molecular diagnostic techniques in all hospitals could help in improving the management of patients with these infections. Resumen: Introducción: Los parechovirus humanos (HPeV) son virus de la familia Picornaviridae, recientemente descritos, a los que se atribuyen cuadros de fiebre sin foco (FSF), sepsis clínica, gastroenteritis, meningitis o encefalitis fundamentalmente en lactantes pequeños. Nuestro objetivo fue describir la epidemiología y las características clínicas de las infecciones por HPeV en nuestro medio. Pacientes y métodos: Estudio multicéntrico prospectivo, llevado a cabo en 12 hospitales a nivel nacional, entre 2013-2015, en niños 3 meses. Todos los pacientes presentaron fiebre, el 45% irritabilidad, el 18,6% exantema y el 14% diarrea. No se observa ninguna alteración específica en las pruebas bioquímicas. El diagnóstico final más frecuente fue FSF (61%) seguido de sepsis clínica (29%). Aunque un 29% de los niños precisaron ingreso en cuidados intensivos, solo un paciente presentó secuelas. Conclusiones: Los HPeV circulan en nuestro país, afectando fundamentalmente a lactantes < 2 meses y se asocian a FSF y sepsis clínica, con un predominio en primavera y verano. Sería de interés implementar las técnicas moleculares de diagnóstico en todos los hospitales para reconocer y manejar adecuadamente estas infecciones.
- Published
- 2018
31. Clinical and Genetic Diagnosis of Nonischemic Sudden Cardiac Death
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Inmaculada de la Torre, Vicente Alcalde Martinez, Beatriz Álvarez Abril, Miriam Jiménez Fernández, Miguel A. Alvarez, Luis Tercedor, Rosa Macías Ruiz, José Manuel Oyonarte Ramírez, Francesca Perin, Elisa María Cabrerizo, Concepción Correa, Silvia Fernández, María del Mar Rodríguez Vázquez del Rey, Rocío García Orta, Juan Jiménez-Jáimez, Mercedes González Molina, and Francisco Bermúdez Jiménez
- Subjects
Male ,Proband ,Pediatrics ,Cardiomyopathy ,Autopsy ,030204 cardiovascular system & hematology ,Sudden cardiac death ,Electrocardiography ,0302 clinical medicine ,030212 general & internal medicine ,Child ,Index case ,Arrhythmogenic Right Ventricular Dysplasia ,Brugada Syndrome ,medicine.diagnostic_test ,High-Throughput Nucleotide Sequencing ,General Medicine ,Middle Aged ,Long QT Syndrome ,Phenotype ,Cardiology ,Female ,Cardiomyopathies ,Adult ,Cardiomyopathy, Dilated ,Heart Defects, Congenital ,medicine.medical_specialty ,Adolescent ,Young Adult ,03 medical and health sciences ,Channelopathy ,Internal medicine ,medicine ,Humans ,Family ,Genetic Predisposition to Disease ,Genetic Testing ,Retrospective Studies ,Genetic testing ,business.industry ,Arrhythmias, Cardiac ,Sequence Analysis, DNA ,Cardiomyopathy, Hypertrophic ,medicine.disease ,Death, Sudden, Cardiac ,Ventricular fibrillation ,Exercise Test ,Tachycardia, Ventricular ,Channelopathies ,business - Abstract
Introduction and objectives Nonischemic sudden cardiac death (SCD) is predominantly caused by cardiomyopathies and channelopathies. There are many diagnostic tests, including some complex techniques. Our aim was to analyze the diagnostic yield of a systematic diagnostic protocol in a specialized unit. Methods The study included 56 families with at least 1 index case of SCD (resuscitated or not). Survivors were studied with electrocardiogram, advanced cardiac imaging, exercise testing, familial study, genetic testing and, in some cases, pharmacological testing. Families with deceased probands were studied using the postmortem findings, familial evaluation, and molecular autopsy with next-generation sequencing (NGS). Results A positive diagnosis was obtained in 80.4% of the cases, with no differences between survivors and nonsurvivors ( P = .53). Cardiac channelopathies were more prevalent among survivors than nonsurvivors (66.6% vs 40%, P = .03). Among the 30 deceased probands, the definitive diagnosis was given by autopsy in 7. A diagnosis of cardiomyopathy tended to be associated with a higher event rate in the family. Genetic testing with NGS was performed in 42 index cases, with a positive result in 28 (66.6%), with no differences between survivors and nonsurvivors ( P = .21). Conclusions There is a strong likelihood of reaching a diagnosis in SCD after a rigorous protocol, with a more prevalent diagnosis of channelopathy among survivors and a worse familial prognosis in cardiomyopathies. Genetic testing with NGS is useful and its value is increasing with respect to the Sanger method.
- Published
- 2017
32. Diagnóstico clínico y genético de la muerte súbita cardiaca de origen no isquémico
- Author
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Silvia Fernández, Rocío García Orta, Juan Jiménez-Jáimez, Miguel A. Alvarez, Rosa Macías Ruiz, José Manuel Oyonarte Ramírez, Beatriz Álvarez Abril, Elisa María Cabrerizo, Francesca Perin, Francisco Bermúdez Jiménez, María del Mar Rodríguez Vázquez del Rey, Luis Tercedor, Vicente Alcalde Martinez, Concepción Correa, Miriam Jiménez Fernández, Inmaculada de la Torre, and Mercedes González Molina
- Subjects
0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,business.industry ,Medicine ,030204 cardiovascular system & hematology ,Cardiology and Cardiovascular Medicine ,business ,Humanities - Abstract
Resumen Introduccion y objetivos La muerte subita cardiaca (MSC) de origen no isquemico esta causada predominantemente por miocardiopatias y canalopatias. La bateria de test diagnosticos es amplia e incluye pruebas complejas. El objetivo de nuestro estudio es analizar la rentabilidad diagnostica del estudio etiologico sistematizado de la MSC. Metodos Se estudio a 56 familias con al menos 1 caso indice con MSC (reanimada o no). En los supervivientes se exploro con electrocardiograma, imagen cardiaca avanzada, ergometria, estudio familiar, estudio genetico y, puntualmente, test farmacologicos. En los fallecidos se examino la necropsia, asi como la autopsia molecular con next generation sequencing (NGS), junto con estudio clinico familiar. Resultados El diagnostico se alcanzo en el 80,4% de los casos, sin diferencias entre supervivientes y fallecidos (p = 0,53). Entre los supervivientes, el diagnostico de canalopatia fue mas frecuente que entre los fallecidos (el 66,6 frente al 40%; p = 0,03). De los 30 sujetos fallecidos, en 7 la autopsia aporto un hallazgo concluyente. El diagnostico de miocardiopatia tendia a asociarse con mayor tasa de eventos en la familia. El test genetico con NGS se realizo en 42 de los casos; se obtuvo resultado positivo en 28 (66,6%), sin diferencias entre supervivientes y fallecidos (p = 0,21). Conclusiones La probabilidad de alcanzar el diagnostico en la MSC tras un protocolo exhaustivo es alta, con mayor prevalencia de canalopatias en los supervivientes y un aparente peor pronostico en las miocardiopatias. El test genetico mediante NGS muestra utilidad en casos de MSC e incrementa la rentabilidad respecto al estudio con Sanger.
- Published
- 2017
33. Anemia neonatal grave secundaria a transfusión feto-materna: a propósito de un caso
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Rocío Pérez Crespo, Cristina Menéndez Hernando, Mercedes Fariñas Salto, Rocío Chacón Aguilar, María Cabrerizo Ortiz, Rocío Moreno Novillo, and Raquel Martin Molina
- Subjects
Pediatrics ,medicine.medical_specialty ,Pregnancy ,Fetus ,business.industry ,medicine.medical_treatment ,Incidence (epidemiology) ,Volume overload ,Exchange transfusion ,medicine.disease ,Pediatrics, Perinatology and Child Health ,Etiology ,Medicine ,business ,Complication ,Chronic anemia - Abstract
Fetomaternal transfusion (FMT) is defined by the transfer of fetal blood into the maternal circulation. The incidence of massive FMT is estimated to be approximately 0.2-0.9 % of births. Although a number of etiologies have been associated with FMT, most causes remain unidentified and the pregnancy is usually asymptomatic. The most frequent symptom is the decrease in fetal movements (26 %) in relation to severe anemia. Several diagnostic modalities for FMT are described (Kleihauer stain, flow cytometry). We describe a case of a newborn with chronic anemia secondary to FMT who, after treatment with transfusions of red blood cells, presented volume overload and clinical worsening as a complication. In this case, our patient needed exchange transfusion for definitive improvement without disability.
- Published
- 2019
34. Enterovirus D68-associated respiratory and neurological illness in Spain, 2014?2018
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Carmen Pérez-González, Rubén González-Sanz, Antonio Moreno-Docón, María Pilar Romero, Sonia Pérez-Castro, Maitane Aranzamendi, Irene Taravillo, María Cabrerizo, Almudena Otero, Jordi Reina, Margarita Del Cuerpo, Ana Navascués, and Instituto de Salud Carlos III
- Subjects
0301 basic medicine ,filogenia ,Epidemiology ,humanos ,medicine.disease_cause ,infecciones del tracto respiratorio ,Drug Discovery ,Paralysis ,Respiratory system ,Respiratory Tract Infections ,Phylogeny ,mediana edad ,infecciones por Enterovirus ,Enterovirus ,Aged, 80 and over ,Enterovirus D, Human ,anciano ,Phylogenetic analysis ,parálisis ,Meningoencephalitis ,General Medicine ,Middle Aged ,adulto ,Acute flaccid paralysis ,Hospitalization ,Infectious Diseases ,Child, Preschool ,Bronchitis ,medicine.symptom ,Meningitis ,Adult ,medicine.medical_specialty ,030106 microbiology ,Immunology ,Microbiology ,Article ,03 medical and health sciences ,respiratory infections ,Internal medicine ,Virology ,medicine ,Enterovirus Infections ,Humans ,hospitalización ,enfermedades del sistema nervioso ,Enterovirus D68 ,Aged ,lactante ,business.industry ,phylogenetic analysis ,Respiratory infections ,Outbreak ,Infant ,bronquitis ,enterovirus D68 ,medicine.disease ,030104 developmental biology ,Spain ,Parasitology ,Nervous System Diseases ,business ,acute flaccid paralysis - Abstract
During 2014, enterovirus D68 (EV-D68) outbreaks were described globally, causing severe respiratory diseases in children and, in some cases, subsequent paralysis. In this study, the type characterization of enterovirus (EV) detected in respiratory illnesses and the epidemiology and clinical association of EV-D68 infections in Spain over a five-year period were described. A total of 546 EV-positive samples from hospitalized patients with respiratory infections were included. EV-D68 was the most frequently detected type (46.6%, 191/410 typed EV). Other EV from species A (25.1%), B (27.8%) and C (0.5%) were also identified. EV-D68 infections were more associated with bronchitis while EV-A/B types were more frequent in upper respiratory illness (p < 0.01). EV-D68 was also detected in patients with neurological symptoms (nine meningitis/meningoencephalitis and eight acute flaccid paralysis cases). Phylogenetic analysis of 3?-VP1 region showed most Spanish EV-D68 sequences from 2014 to 2016 belonged to subclades B2/B3, as other American and European strains circulating during the same period. However, those detected in 2017 and 2018 clustered to the emerged subclade D1. In summary, different EV can cause respiratory infections but EV-D68 was the most prevalent, with several strains circulating in Spain at least since 2014. Association between EV-D68 infection and neurological disease was also described., Dr. R. Gonzalez-Sanz is supported by a grant from the Instituto de Salud Carlos III [grant number PI15CIII-00020]. This study was partially supported by the same grant.
- Published
- 2019
35. First Cases of Severe Flaccid Paralysis Associated With Enterovirus D68 Infection in Spain, 2015–2016
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María Cabrerizo, Paula Madurga-Revilla, Víctor del Campo, Andrés Antón, Gerardo Suárez, Carlos Rodrigo, Almudena Otero, A. Martínez-Sapiña, Francina Munell, Nuria Rabella, J.P. García-Íñiguez, Josefa Masa-Calles, Sonia Pérez, and Alfonso Amado
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,Acute flaccid paralysis ,Pediatrics ,medicine.medical_specialty ,Flaccid paralysis ,Myelitis ,Enterovirus D ,03 medical and health sciences ,0302 clinical medicine ,Enterovirus Infections ,Paralysis ,medicine ,Humans ,Enterovirus d68 infection ,030212 general & internal medicine ,enterovirus infection ,Enterovirus D, Human ,severe neurologic diseases ,business.industry ,Brain ,Infant ,virus diseases ,medicine.disease ,Magnetic Resonance Imaging ,Virology ,pediatric ,030104 developmental biology ,Infectious Diseases ,Spinal Cord ,Spain ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,surveillance ,medicine.symptom ,business ,Enterovirus D68 ,Spinal cord pathology - Abstract
Enterovirus D68 was known to be the cause of mild to severe respiratory infections, but in the last few years, it has also been associated with myelitis and paralysis. This report describes the first Enterovirus D68 detections in acute flaccid paralysis cases occurring between December 2015 and March 2016 in Spain.
- Published
- 2017
36. Acute flaccid paralysis (AFP) surveillance: challenges and opportunities from 18 years’ experience, Spain, 1998 to 2015
- Author
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Celia Marín, Sara García-Hernández, Carmen Malo, Henar Marcos, Juan García Gutiérrez, Visitación García Ortúzar, Noemí López-Perea, María Cabrerizo, Manuel García Cenoz, Josefa Masa-Calles, Beatriz Fernández-Martínez, Ana Rivas, A. Martinez, Isabel Losada, José M. Arteagoitia, María de Viarce Torres de Mier, Amelia Fuentes, Natividad Abadía, Margarita Portell, Virtudes Gallardo-García, Daniel Castrillejo, Alba Nieto, Mario Margolles, Nuria Torner, Nuria Rabella, Aniceto Blasco, and Universitat de Barcelona
- Subjects
Male ,0301 basic medicine ,Pediatrics ,Epidemiology ,Communicable diseases ,medicine.disease_cause ,Surveillance and Outbreak Report ,Disease Outbreaks ,0302 clinical medicine ,030212 general & internal medicine ,Child ,Enterovirus ,Vaccine preventable disease ,Public health ,education.field_of_study ,Surveillance ,enterovirus ,Poliovirus ,Poliomyelitis ,Poliovirus Vaccines ,Child, Preschool ,Population Surveillance ,surveillance ,Acute flaccid paralysis surveillance ,Female ,Acute flaccid paralysis ,medicine.medical_specialty ,Adolescent ,poliomyelitis eradication ,030106 microbiology ,Population ,Poliomielitis ,Laboratory testing ,03 medical and health sciences ,Virology ,Poliomyelitis eradication ,medicine ,Humans ,Paralysis ,Disease Eradication ,education ,Disease Notification ,business.industry ,vaccine preventable disease ,Public Health, Environmental and Occupational Health ,Infant ,Malalties infeccioses ,medicine.disease ,Salut pública ,Spain ,Poliovirus Vaccine, Oral ,business ,Notification rate ,acute flaccid paralysis surveillance - Abstract
Acute flaccid paralysis (AFP) surveillance is key for global polio eradication. It allows detecting poliovirus (PV) reintroductions from endemic countries. This study describes AFP surveillance in Spain from 1998 to 2015. During this time, 678 AFP cases were reported to the Spanish National Surveillance Network. The mean notification rate was 0.58 AFP cases/100,000 population under 15 years old (range: 0.45/100,000-0.78/100,000). Two periods (P) are described: P1 (1998-2006) with the AFP notification rate ranging from 0.66/100,000 to 0.78/100,000, peaking in 2001 (0.84/100,000); and P2 (2007-2015) when the AFP rate ranged from 0.43/100,000 to 0.57/100,000, with the lowest rate in 2009 (0.31/100,000). No poliomyelitis cases were caused by wild PV infections, although two Sabin-like PVs and one imported vaccine-derived PV-2 were detected. Overall, 23 (3.4%) cases met the hot case definition. Most cases were clinically diagnosed with Guillain-Barré syndrome (76.9%; 504/655). The adequate stool collection rate ranged from 33.3% (7/21) to 72.5% (29/40). The annual proportion of AFP cases with non-polio enterovirus findings varied widely across the study period. AFP surveillance with laboratory testing for non-polio enteroviruses must be maintained and enhanced both to monitor polio eradication and to establish sensitive surveillance for prompt detection of other enteroviruses causing serious symptoms. We sincerely thank Odorina Tello, María Victoria Martínez de Aragón and Gloria Trallero for their valuable contributions to the implementation and development of the National Action Plan Aimed at the Achievement of the Certificate of Polio Eradication, and especially for coordinating the Acute Flaccid Paralysis Surveillance System and the Enterovirus Laboratory Networks in Spain. Sí
- Published
- 2018
37. Molecular epidemiology of enterovirus and parechovirus infections according to patient age over a 4-year period in Spain
- Author
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María Pilar Romero, María Cabrerizo, María José Mellado Peña, Antonio Moreno-Docón, Gloria Trallero, Almudena Otero, Sonia Rey-Cao, María Díaz-Cerio, David Tarragó, Inés Martinez-Rienda, Carmen Muñoz-Almagro, Nuria Rabella, and Cristina Calvo
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Myocarditis ,Molecular epidemiology ,030106 microbiology ,Biology ,medicine.disease_cause ,medicine.disease ,biology.organism_classification ,Virology ,03 medical and health sciences ,Infectious Diseases ,Patient age ,Epidemiology ,Parechovirus ,medicine ,Enterovirus ,Meningitis ,Encephalitis - Abstract
The epidemiology and clinical association of enterovirus (EV) and parechovirus (HPeV) infections, as well as the type-distribution-according-to-age, were determined during a 4-year study period in Spain. During 2010–2013, a total of 21,832 clinical samples were screened for EV and the detection frequency was 6.5% (1,430). Of the total EV-negative samples, only 1,873 samples from 2011 to 2013 were available for HPeV testing. HPeV was detected in 42 (2%) of them. Positive samples were genotyped using PCR and sequencing. EV infections occurred in all age groups of patients: neonates (17%), children 28 days to 2 years (29%), children 2–14 years (40%), and adults (14%). Thirty-four different EV types were identified. HPeV infections were detected exclusively in infants 2 years and adults (P
- Published
- 2016
38. Infecciones respiratorias agudas comunitarias causadas por enterovirus en la población pediátrica
- Author
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Jordi Reina, María Cabrerizo, and Francisco Aliaga
- Subjects
0301 basic medicine ,Microbiology (medical) ,Pediatrics ,medicine.medical_specialty ,Respiratory tract infections ,Viral Epidemiology ,business.industry ,030106 microbiology ,Coxsackievirus Infections ,medicine.disease ,medicine.disease_cause ,03 medical and health sciences ,Pneumonia ,0302 clinical medicine ,Bronchiolitis ,030225 pediatrics ,medicine ,Enterovirus ,Respiratory system ,business ,Paediatric population - Published
- 2017
39. Utility of FilmArray Meningitis/Encephalitis Panel during Outbreak of Brainstem Encephalitis Caused by Enterovirus in Catalonia in 2016
- Author
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Ana Valero-Rello, Dídac Casas-Alba, Cristian Launes, Claudia Fortuny, María Cabrerizo, and Carmen Muñoz-Almagro
- Subjects
0301 basic medicine ,Microbiology (medical) ,Enterovirus Infections ,030106 microbiology ,medicine.disease_cause ,Polymerase Chain Reaction ,Disease Outbreaks ,03 medical and health sciences ,Meningitis/encephalitis ,Multiplex polymerase chain reaction ,Humans ,Medicine ,Child ,Letter to the Editor ,Cerebrospinal Fluid ,business.industry ,Outbreak ,medicine.disease ,Virology ,Brainstem encephalitis ,Spain ,Child, Preschool ,Encephalitis ,Enterovirus ,business ,Meningitis ,Brain Stem - Abstract
We read with interest the article by Leber et al. ([1][1]). The authors reported the performance of the FilmArray meningitis/encephalitis (FA-M/E) panel in a multicenter evaluation that included a large number of infants. Enterovirus (EV) was the main detection, and they described very high positive
- Published
- 2017
40. Viral gastroenteritis in hospitalized patients: Evaluation of immunochromatographic methods for rapid detection in stool samples
- Author
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María Francisca Portero-Azorín, María Muñoz-Algarra, María Cabrerizo, Rubén González-Sanz, Purificación Higueras, Maria José Amaro, and Lydia González-Serrano
- Subjects
Rotavirus ,medicine.medical_specialty ,Adolescent ,viruses ,medicine.disease_cause ,Sensitivity and Specificity ,Rapid detection ,Chromatography, Affinity ,Adenoviridae ,Astrovirus ,Adenovirus Infections, Human ,Feces ,fluids and secretions ,Virology ,Epidemiology ,Enterovirus Infections ,Adenovirus ,Humans ,Medicine ,Child ,Pathogen ,Caliciviridae Infections ,biology ,business.industry ,Norovirus ,Infant ,virus diseases ,biology.organism_classification ,Gastroenteritis ,Hospitalization ,PCR ,Infectious Diseases ,Virus Diseases ,Child, Preschool ,Etiology ,Enterovirus ,Reagent Kits, Diagnostic ,Immunochromatographic test ,business - Abstract
Background . Viral infections are recognized as the most common cause of acute gastroenteritis (AGE). Virus detection by immune analytical methods is recommended for diagnosis because of its simplicity and low cost. Objectives . Two commercial immunochromatographic (ICG) techniques (Materlab) for rapid detection of rotavirus/adenovirus and norovirus respectively, were evaluated by comparison to the results obtained using PCR methods. In addition, clinical and epidemiologic characteristics of AGE infections have been described. Study design . A total of 100 faecal samples collected from patients with AGE (84% children) admitted into a Spanish Hospital between February and July 2018, were studied for rotavirus-A, adenovirus and norovirus GI/GII by the ICG tests as well as by PCR and sequencing. Other enteric viruses (enterovirus and astrovirus) were investigated by PCR methods. Gastrointestinal bacteria and parasites were also tested. Results . Evaluated ICG tests yielded high specificity (>97%). Sensitivity values were high for rotavirus/adenovirus (>80%) but lower for norovirus (57%). Overall, and taking into account coinfections, viruses (32%), bacteria (14%) and parasites (1%) could be detected. Rotavirus-A were the most frequently identified viruses (16%), followed by enterovirus (12%), norovirus (4%), adenovirus 41 (4%) and astrovirus (1%). In five vaccinated children, a rotavirus was detected. Conclusions . ICG technique is a useful tool for the routine diagnosis of AGE infections at hospital, but for surveillance and epidemiological studies, it is needed the use of amplification and sequencing methods, which also allow monitoring of new strains or variants emergence. In this study, an etiological pathogen was determined only in 44% of samples.
- Published
- 2020
41. Enterovirus neurological disease and bacterial coinfection in very young infants with fever
- Author
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Paula Gallardo, María Pilar Romero, Cristina Calvo, Fernando Baquero-Artigao, Teresa Del Rosal, María Cabrerizo, Ana Méndez-Echeverría, Talía Sainz, Patricia Torija, and Sara Bellon
- Subjects
Male ,Fever ,medicine.disease_cause ,Virus ,Sepsis ,03 medical and health sciences ,0302 clinical medicine ,Meningoencephalitis ,030225 pediatrics ,Virology ,Enterovirus Infections ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Bacterial disease ,biology ,Coinfection ,Incidence ,C-reactive protein ,Infant, Newborn ,Infant ,Bacterial Infections ,medicine.disease ,Infectious Diseases ,Spain ,Immunology ,biology.protein ,Enterovirus ,Female ,Meningitis - Abstract
Background Very little information exists on simultaneous infections by viruses and bacteria in infants with fever without source (FWS). Objectives To investigate the incidence of bacterial coinfection in infants up to 3 months of age with neurological viral infection. Study design Prospective study performed in infants below 90 days of age attending the emergency room of two public hospitals in Spain for FWS. Those who had viral screening performed in CSF, together with blood, CSF and urine cultures were included. Herpes virus, EV and HPeV detection in CSF was performed by PCR. Coinfections between viruses in CSF and serious bacterial infections were described. Results 119 Infants less than 90 days of age were recruited. Forty-five (38%) had viral infection of the central nervous system, and in 8 of them (17.7%) we found a concurrent bacterial infection: 7 urinary tract infections (UTI) and 1 sepsis. In all cases, the virus identified in CSF was EV. Conclusions Bacterial infections were frequent in young infants with viral neurological infections associated to EV. Urinary tract infection was the most common bacterial disease.
- Published
- 2016
42. [The importance of epidemiological surveillance of emerging enteroviruses]
- Author
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Alfonso, Amado Puentes and María, Cabrerizo Sanz
- Subjects
Tertiary Care Centers ,Spain ,Enterovirus Infections ,Humans ,Disease Outbreaks ,Enterovirus - Published
- 2018
43. Epidemiological analysis of acute respiratory infections caused by enterovirus D68 clade A, subclade A1 in the adult population
- Author
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Jordi Reina, María Cabrerizo, and Ester del Barrio
- Subjects
Adult ,Enterovirus D, Human ,Male ,Adolescent ,Virulence ,Age Factors ,Middle Aged ,Disease Outbreaks ,Young Adult ,Spain ,Enterovirus Infections ,Humans ,Female ,Respiratory Tract Infections ,Aged - Published
- 2018
44. [Severe neonatal anemia due to fetomaternal hemorrhage: an ilustrative case]
- Author
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Cristina, Menéndez Hernando, Rocío, Chacón Aguilar, Mercedes, Fariñas Salto, Rocío, Pérez Crespo, Raquel, Martin Molina, Rocío, Moreno Novillo, and María, Cabrerizo Ortiz
- Subjects
Male ,Adolescent ,Anemia, Neonatal ,Pregnancy ,Exchange Transfusion, Whole Blood ,Infant, Newborn ,Humans ,Female ,Severity of Illness Index ,Fetomaternal Transfusion - Abstract
Fetomaternal transfusion (FMT) is defined by the transfer of fetal blood into the maternal circulation. The incidence of massive FMT is estimated to be approximately 0.2-0.9 % of births. Although a number of etiologies have been associated with FMT, most causes remain unidentified and the pregnancy is usually asymptomatic. The most frequent symptom is the decrease in fetal movements (26 %) in relation to severe anemia. Several diagnostic modalities for FMT are described (Kleihauer stain, flow cytometry). We describe a case of a newborn with chronic anemia secondary to FMT who, after treatment with transfusions of red blood cells, presented volume overload and clinical worsening as a complication. In this case, our patient needed exchange transfusion for definitive improvement without disability.La transfusión feto-materna es el paso de eritrocitos fetales a la circulación materna. Cuando es masiva, tiene una incidencia aproximada del 0,2-0,9 %. Generalmente, se desconoce el agente desencadenante, pero, en ocasiones, se pueden identificar factores de riesgo. En el embarazo, suele ser asintomática; el síntoma más frecuente es la disminución de los movimientos fetales (el 26 %) en relación con la anemia grave. Se diagnostica mediante la detección de hemoglobina fetal en la sangre materna (test de Kleihauer o citometría de flujo). Se presenta a un recién nacido con anemia crónica secundaria a la transfusión fetomaterna, que, después del tratamiento con transfusión de concentrado de hematíes, tuvo como complicación síntomas de sobrecarga de volumen y empeoramiento clínico. Tras la realización de una exanguinotransfusión, evolucionó favorablemente, sin secuelas.
- Published
- 2018
45. Molecular surveillance of norovirus, 2005–16 : an epidemiological analysis of data collected from the NoroNet network
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Vito Martella, Mateja Poljšak-Prijatelj, Mia Brytting, Martin C.W. Chan, Alexander T. Podkolzin, Janko van Beek, Katia Ambert-Balay, Leena Maunula, Miao Jin, Georgina McAllister, Janet Mans, Reimar Johne, Joanne Hewitt, Nadine Botteldoorn, Gábor Reuter, Haider Al-Hello, Fiona Cloak, Harry Vennema, Lasse Dam Rasmussen, Gráinne Tuite, Miranda de Graaf, María Cabrerizo, Susana Guix, Hubert G. M. Niesters, Javier Buesa, Marion Koopmans, Nobuhiro Iritani, Sandra Niendorf, Ilaria Di Bartolo, Ingeborg Lederer, David J. Allen, Annelies Kroneman, Department of Viroscience [Rotterdam, The Netherlands], Erasmus University Medical Center [Rotterdam] (Erasmus MC), National Institute of Public Health and the Environment (NIPHE), Department of Integrative Physiology, University of Colorado [Boulder], Laboratoire de sérologie-virologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Scientific Service of Foodborne Pathogens [Brussels], Institute of Public Health [Brussels], Microbial Typing Unit [Stockholm], The Public Health Agency of Sweden, Departamento de Microbiología, Facultad de Medicina, Universitat de València (UV), Enterovirus and Viral Gastroenteritis [Madrid], Instituto de Salud Carlos III [Madrid] (ISC), Department of Microbiology, the University of Hong Kong, The University of Hong Kong (HKU), Health Protection Surveillance Centre, Health Protection Surveillance Centre (HPSC), Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita [Rome], Enteric Virus Laboratory [Barcelona], University of Barcelona, Norovirus Reference Laboratory [Porirua], Institute of Environmental Science and Research (ESR), Department of Microbiology [Osaka], Osaka Institute of Public Health, National Institute for Viral Disease Control and Prevention, Department of Biological Safety, Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Austrian Agency for Health and Food Safety (AGES), Department of Medical Virology, University of Pretoria [South Africa], Sanità e Benessere degli Animali, Università di Bari, Facoltà di Medicina Veterinaria, Department of Food Hygiene and Environmental Health [Helsinki], Faculty of Veterinary Medicine [Helsinki], University of Helsinki-University of Helsinki, Royal Infirmary of Edinburgh, National Institute for Health and Welfare [Helsinki], Centre National de Référence des virus entériques [CHU de Dijon] (CNR virus entériques), Department of Pathogen Molecular Biology [London, UK], London School of Hygiene and Tropical Medicine (LSHTM), National Institute for Health Research (NIHR), European Union's Horizon 2020 grant COMPARE, ZonMw TOP grant, the Virgo Consortium funded by the Dutch Government, and the Hungarian Scientific Research Fund., Erasmus University Medical Center [Rotterdam], National Institute of Public Health and the Environment ( NIPHE ), University of Colorado Boulder [Boulder], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Procédés Alimentaires et Microbiologiques [Dijon] ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Universitat de València ( UV ), Instituto de Salud Carlos III, The University of Hong Kong ( HKU ), Institute of Environmental Science and Research, Federal Institute for Risk Assessment, AGES, Austrian Agency for Health and Food Safety - Austrian Agency for Health and Food Safety , Autriche., Austrian Agency for Health and Food Safety, Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, University of Edinburgh, Centre National de Référence des virus entériques [CHU de Dijon] ( CNR virus entériques ), Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine ( LSHTM ), Erasmus University Medical Centre Rotterdam [Rotterdam], Virology, Leena Maunula / Principal Investigator, Food Hygiene and Environmental Health, and Food and Environmental Virology Research Group
- Subjects
0301 basic medicine ,Databases, Factual ,viruses ,VARIANTS ,medicine.disease_cause ,Disease Outbreaks ,EMERGENCE ,fluids and secretions ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Epidemiology ,Genotype ,TOOL ,media_common ,Caliciviridae Infections ,Molecular Epidemiology ,virus diseases ,respiratory system ,3. Good health ,Gastroenteritis ,[ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Infectious Diseases ,Geography ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,RNA, Viral ,[ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,OUTBREAKS ,medicine.medical_specialty ,EUROPE ,TRANSMISSION ,VIRUSES ,[ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Genetic drift ,Environmental health ,medicine ,media_common.cataloged_instance ,Humans ,European union ,Retrospective Studies ,Genetic diversity ,Molecular epidemiology ,Norovirus ,Outbreak ,Genetic Variation ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,ADULTS ,digestive system diseases ,EVOLUTION ,030104 developmental biology ,3121 General medicine, internal medicine and other clinical medicine ,human activities - Abstract
BACKGROUND: The development of a vaccine for norovirus requires a detailed understanding of global genetic diversity of noroviruses. We analysed their epidemiology and diversity using surveillance data from the NoroNet network.METHODS: We included genetic sequences of norovirus specimens obtained from outbreak investigations and sporadic gastroenteritis cases between 2005 and 2016 in Europe, Asia, Oceania, and Africa. We genotyped norovirus sequences and analysed sequences that overlapped at open reading frame (ORF) 1 and ORF2. Additionally, we assessed the sampling date and country of origin of the first reported sequence to assess when and where novel drift variants originated.FINDINGS: We analysed 16 635 norovirus sequences submitted between Jan 1, 2005, to Nov 17, 2016, of which 1372 (8·2%) sequences belonged to genotype GI, 15 256 (91·7%) to GII, and seven (INTERPRETATION: Continuous changes in the global norovirus genetic diversity highlight the need for sustained global norovirus surveillance, including assessment of possible immune escape and evolution by recombination, to provide a full overview of norovirus epidemiology for future vaccine policy decisions.FUNDING: European Union's Horizon 2020 grant COMPARE, ZonMw TOP grant, the Virgo Consortium funded by the Dutch Government, and the Hungarian Scientific Research Fund.
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- 2018
46. La importancia de la vigilancia epidemiológica de los enterovirus emergentes
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María Cabrerizo Sanz and Alfonso Amado Puentes
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Enterovirus Infections ,business.industry ,medicine.disease_cause ,Virology ,Pediatrics ,RJ1-570 ,Disease Outbreaks ,Tertiary Care Centers ,Spain ,Pediatrics, Perinatology and Child Health ,medicine ,Enterovirus ,Humans ,business - Abstract
Sí
- Published
- 2019
47. Prospection of Hepatitis E Virus in Human, Swine and Sewage Samples in Spain
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Tajada P, Echevarria Jm, M. Fogeda, Leonardo Calle García, Rosa M. Sánchez, Martin M, Sampedro A, Gloria Trallero, María Cabrerizo, Ana Avellón, Schuller de Santos C, Serrano E, C.G. Cilla, and Garcia Arevalo C
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Hepatitis ,medicine.medical_specialty ,viruses ,animal diseases ,virus diseases ,Biology ,medicine.disease_cause ,medicine.disease ,Pharmaceutical microbiology ,Virology ,digestive system diseases ,Virus ,Microbiology ,Medical microbiology ,Hepatitis E virus ,Parasitology ,Molecular microbiology ,medicine ,Microbial genetics - Abstract
Hepatitis E virus HEV has been detected in Spain among patients with acute hepatitis swine livestock wild fauna and urban sewage but prospective studies have been scarce The incidence of the infection among humans and the mechanisms for acquisition of local HEV strains are unknown Serum samples from prospectively selected patients displaying liver alterations were tested for IgG and IgM antibody to HEV anti HEV and for HEV RNA HEV RNA was investigated in stool samples from piglets and in sewage samples from a single region and anti HEV was tested in serum samples from piglets from the same farms Acute HEV infection was identified in six patients and evidence of contact with HEV in the past but unrelated to the ongoing liver alterations was obtained in eight Two imported and four autochthonous cases of acute infection were recorded Most autochthonous cases were found in the Basque Country where swine livestock is not of economic importance HEV RNA was detected in stool samples from three piglets aged weeks from a single farm and anti HEV was found among piglets from all farms at rates ranging overall from to but from to among the oldest ones weeks old All sewage samples tested negative for HEV RNA The results show that locally acquired human HEV infection might be significantly more frequent in the Northern regions of Spain and that swine livestock might not be in the origin of most of these infections
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- 2017
48. Outbreak of brainstem encephalitis associated with enterovirus-A71 in Catalonia, Spain (2016): a clinical observational study in a children's reference centre in Catalonia
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Dídac Casas-Alba, Carles Luaces, Thaís Armangue, Claudia Fortuny, Carlos Ortez, M.F. de Sevilla, Juan José García-García, María Cabrerizo, Jordi Muchart, Iolanda Jordan, Cristian Launes, Rubén González-Sanz, Ana Valero-Rello, Irene Barrabeig, and Carmen Muñoz-Almagro
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Male ,0301 basic medicine ,Microbiology (medical) ,Pathology ,medicine.medical_specialty ,Pediatrics ,Encephalomyelitis ,030106 microbiology ,Anti-Inflammatory Agents ,medicine.disease_cause ,Procalcitonin ,Disease Outbreaks ,law.invention ,03 medical and health sciences ,Lethargy ,0302 clinical medicine ,law ,Enterovirus Infections ,medicine ,Humans ,Encephalitis, Viral ,030212 general & internal medicine ,Enterovirus ,Molecular Epidemiology ,business.industry ,Infant ,Outbreak ,Aseptic meningitis ,General Medicine ,medicine.disease ,Intensive care unit ,Enterovirus A, Human ,EV-A71 ,Infectious Diseases ,Spain ,Child, Preschool ,Molecular epidemiology ,Types ,Encephalitis ,Female ,business ,Brainstem ,Brain Stem - Abstract
OBJECTIVES: To describe the characteristics of an outbreak of brainstem encephalitis and encephalomyelitis related to enterovirus (EV) infection in Catalonia (Spain), a setting in which these manifestations were uncommon. METHODS: Clinical and microbiological data were analysed from patients with neurological symptoms associated with EV detection admitted to a reference paediatric hospital between April and June 2016. RESULTS: Fifty-seven patients were included. Median age was 27.7 months (p25-p75 17.1-37.6). Forty-one (72%) were diagnosed with brainstem encephalitis, seven (12%) with aseptic meningitis, six (11%) with encephalitis, and three (5%) with encephalomyelitis (two out of three with cardiopulmonary failure). Fever, lethargy, and myoclonic jerks were the most common symptoms. Age younger than 12 months, higher white-blood-cell count, and higher procalcitonin levels were associated with cardiopulmonary failure. Using a PAN-EV real-time PCR, EV was detected in faeces and/or nasopharyngeal aspirate in all the patients, but it was found in cerebrospinal fluid only in patients with aseptic meningitis. EV was genotyped in 47 out of 57 and EV-A71 was identified in 40 out of 47, being the only EV type found in patients with brainstem symptoms. Most of the detected EV-A71 strains were subgenogroup C1. Intravenous immunoglobulins were used in 34 patients. Eight cases (14%) were admitted to the intensive care unit. All the patients but three, those with encephalomyelitis, showed a good clinical course and had no significant sequelae. No deaths occurred. CONCLUSIONS: The 2016 outbreak of brainstem encephalitis in Catalonia was associated with EV-A71 subgenogroup C1. Despite the clinical manifestations of serious disease, a favourable outcome was observed in the majority of patients.
- Published
- 2017
49. Vigilancia de la Parálisis Flácida Aguda y Vigilancia de Enterovirus, 2016 PLAN DE ACCIÓN EN ESPAÑA PARA LA ERRADICACIÓN DE LA POLIOMIELITIS
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López-Perea, Noemí, Masa-Calles, Josefa, Mier, María De Viarce Torres De, Martínez, Beatriz Fernández, and Sanz, María Cabrerizo
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- 2017
- Full Text
- View/download PDF
50. Molecular epidemiology of enterovirus 71, coxsackievirus A16 and A6 associated with hand, foot and mouth disease in Spain
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I. López-Miragaya, Carmen Muñoz-Almagro, M. Domínguez-Gil, C. Pérez, María Cabrerizo, Irene González, J. Reina, Gloria Trallero, José María Eiros, Juan Emilio Echevarría, E. del Amo, David Tarragó, and Almudena Otero
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Male ,medicine.disease_cause ,molecular epidemiology ,Disease Outbreaks ,Genotype ,Enterovirus 71 ,Prevalence ,Coxsackievirus ,phylogenetic analyses ,Child ,Phylogenetic analyses ,Phylogeny ,biology ,Foot-and-mouth disease ,Incidence (epidemiology) ,Epidemiología molecular ,General Medicine ,Middle Aged ,Infectious Diseases ,Molecular epidemiology ,Child, Preschool ,Female ,Adult ,Microbiology (medical) ,Genotyping ,Adolescent ,Molecular Sequence Data ,Young Adult ,stomatognathic system ,32 Ciencias Médicas ,medicine ,Humans ,Infant, Newborn ,Outbreak ,Infant ,Fiebre aftosa ,biology.organism_classification ,medicine.disease ,Virology ,HFMD ,Enterovirus A, Human ,Enterovirus C, Human ,genotyping ,Spain ,Enterovirus ,Capsid Proteins ,Hand, Foot and Mouth Disease - Abstract
Producción Científica, Hand, foot and mouth disease (HFMD) is a childhood illness frequently caused by genotypes belonging to the enterovirus A species, including coxsackievirus (CV)-A16 and enterovirus (EV)-71. Between 2010 and 2012, several outbreaks and sporadic cases of HFMD occurred in different regions of Spain. The objective of the present study was to describe the enterovirus epidemiology associated with HFMD in the country. A total of 80 patients with HFMD or atypical rash were included. Detection and typing of the enteroviruses were performed directly in clinical samples using molecular methods. Enteroviruses were detected in 53 of the patients (66%). CV-A6 was the most frequent genotype, followed by CV-A16 and EV-71, but other minority types were also identified. Interestingly, during almost all of 2010, CV-A16 was the only causative agent of HFMD but by the end of the year and during 2011, CV-A6 became predominant, while CV-A16 was not detected. In 2012, however, both CV-A6 and CV-A16 circulated. EV-71 was associated with HFMD symptoms only in three cases during 2012. All Spanish CV-A6 sequences segregated into one major genetic cluster together with other European and Asian strains isolated between 2008 and 2011, most forming a particular clade. Spanish EV-71 strains belonged to subgenogroup C2, as did most of the European sequences circulated. In conclusion, the recent increase of HFMD cases in Spain and other European countries has been due to a larger incidence of circulating species A enteroviruses, mainly CV-A6 and CV-A16, and the emergence of new genetic variants of these viruses.
- Published
- 2014
- Full Text
- View/download PDF
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