1. The role of a class III gibberellin 2-oxidase in tomato internode elongation
- Author
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, University of California, Davis, National Science Foundation, EEUU, National Institutes of Health, EEUU, National Institute of Food and Agriculture, EEUU, Ministerio de Economía y Competitividad, Lavelle, A., Gath, N., Devisetty, U., Carrera Bergua, Esther, Lopez Diaz, Isabel, Blazquez Rodriguez, Miguel Angel, Maloof, J., Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, University of California, Davis, National Science Foundation, EEUU, National Institutes of Health, EEUU, National Institute of Food and Agriculture, EEUU, Ministerio de Economía y Competitividad, Lavelle, A., Gath, N., Devisetty, U., Carrera Bergua, Esther, Lopez Diaz, Isabel, Blazquez Rodriguez, Miguel Angel, and Maloof, J.
- Abstract
[EN] A network of environmental inputs and internal signaling controls plant growth, development and organ elongation. In particular, the growth-promoting hormone gibberellin (GA) has been shown to play a significant role in organ elongation. The use of tomato as a model organism to study elongation presents an opportunity to study the genetic control of internode-specific elongation in a eudicot species with a sympodial growth habit and substantial internodes that can and do respond to external stimuli. To investigate internode elongation, a mutant with an elongated hypocotyl and internodes but wild-type petioles was identified through a forward genetic screen. In addition to stem-specific elongation, this mutant, named tomato internode elongated -1 (tie-1) is more sensitive to the GA biosynthetic inhibitor paclobutrazol and has altered levels of intermediate and bioactive GAs compared with wild-type plants. The mutation responsible for the internode elongation phenotype was mapped to GA2oxidase 7, a class III GA 2-oxidase in the GA biosynthetic pathway, through a bulked segregant analysis and bioinformatic pipeline, and confirmed by transgenic complementation. Furthermore, bacterially expressed recombinant TIE protein was shown to have bona fide GA 2-oxidase activity. These results define a critical role for this gene in internode elongation and are significant because they further the understanding of the role of GA biosynthetic genes in organ-specific elongation.
- Published
- 2018